一株新美鞭菌LGM-ZA13及其在制备降解香豆素的草木樨饲草添加剂中的应用

The degradation of coumarin in sweet clover by fermentation broth of *Syzygium buergerianum* LGM-ZA13 solved the problem of degradation of toxic substances in sweet clover, improved the forage application value of sweet clover, and alleviated the problem of insufficient high-quality forage resources.

CN116515638BActive Publication Date: 2026-07-17NANJING AGRICULTURAL UNIVERSITY

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
NANJING AGRICULTURAL UNIVERSITY
Filing Date
2023-03-31
Publication Date
2026-07-17

AI Technical Summary

Technical Problem

The presence of coumarin in sweet clover can cause poisoning in ruminants, and current technologies are unable to effectively degrade it, affecting its application as a high-quality forage.

Method used

Osmanthus fragrans was pretreated with fermentation broth of *Eriocaulon buergerianum* LGM-ZA13 to degrade coumarin using its secreted acetylylanase. The fermentation conditions were anaerobic fermentation for 72-196 h at 39℃. The fermentation broth contained basal culture medium, trace element buffer, cell-free rumen fluid, and redox agents.

Benefits of technology

It significantly improved the degradation rate of coumarin in sweet clover, from 47.2% to 80.7%, providing a safety guarantee for the application of sweet clover as forage and alleviating the current shortage of high-quality forage resources.

✦ Generated by Eureka AI based on patent content.

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Abstract

本发明公开了一株新美鞭菌LGM‑ZA13及其在制备降解香豆素的草木樨饲草添加剂中的应用。所述的新美鞭菌LGM‑ZA13分类命名为新美鞭菌Neocallimastix sp.,保藏编号为CGMCC No.17593。所述新美鞭菌LGM‑ZA13的发酵菌液可以用于草木樨饲草中降解抗营养因子香豆素,且使用方法简单,只需将草木樨与发酵液混合后杀菌,再加入发酵菌液厌氧发酵即可获得低香豆素含量的草木樨,该制备方法工艺程序简单、可操作性强,可显著降低香豆素的含量,为草木樨作为优质饲草饲喂反刍动物提供新的方法。
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