一株新美鞭菌LGM-ZA13及其在制备降解香豆素的草木樨饲草添加剂中的应用
The degradation of coumarin in sweet clover by fermentation broth of *Syzygium buergerianum* LGM-ZA13 solved the problem of degradation of toxic substances in sweet clover, improved the forage application value of sweet clover, and alleviated the problem of insufficient high-quality forage resources.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- NANJING AGRICULTURAL UNIVERSITY
- Filing Date
- 2023-03-31
- Publication Date
- 2026-07-17
AI Technical Summary
The presence of coumarin in sweet clover can cause poisoning in ruminants, and current technologies are unable to effectively degrade it, affecting its application as a high-quality forage.
Osmanthus fragrans was pretreated with fermentation broth of *Eriocaulon buergerianum* LGM-ZA13 to degrade coumarin using its secreted acetylylanase. The fermentation conditions were anaerobic fermentation for 72-196 h at 39℃. The fermentation broth contained basal culture medium, trace element buffer, cell-free rumen fluid, and redox agents.
It significantly improved the degradation rate of coumarin in sweet clover, from 47.2% to 80.7%, providing a safety guarantee for the application of sweet clover as forage and alleviating the current shortage of high-quality forage resources.
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Figure CN116515638B_ABST