DNA检测传感器、系统、制造方法、检测方法及用途
By combining graphene transistors with DNA hydrogel functionalized gate electrodes based on the CRISPR-Cas12a system, the problems of long processing time and high cost in existing nucleic acid diagnostic methods have been solved, achieving label-free, rapid, and highly specific DNA detection suitable for portable devices.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- 杭州领挚科技有限公司
- Filing Date
- 2023-05-05
- Publication Date
- 2026-07-17
AI Technical Summary
Existing nucleic acid diagnostic methods are time-consuming, costly, and require large equipment and cumbersome sample pretreatment, which cannot meet the needs for portable, low-cost, and immediate ultra-trace detection. Furthermore, the multi-step nucleic acid amplification process may affect the accurate quantification of the target analyte.
A label-free, highly sensitive DNA detection platform was constructed by combining a graphene transistor-based biosensor with the CRISPR-Cas12a system. The platform utilizes a DNA hydrogel-functionalized gate electrode and graphene channels to achieve capacitive response and directly detect DNA sequences.
It achieves label-free, rapid, and highly specific DNA detection with fast response speed and large signal differences. It can directly detect single-stranded and double-stranded DNA, is suitable for portable devices, reduces detection costs and time, and improves detection sensitivity and stability.
Smart Images
Figure CN116519767B_ABST