一种串联羊源大肠杆菌毒力基因融合蛋白的制备和应用
By screening and tandemly expressing five virulence genes of sheep-derived Escherichia coli using genetic engineering technology, the problem of insufficient antigenic epitope selection in existing technologies has been solved, enabling the efficient preparation of vaccines against sheep Escherichia coli disease with strong immune response and protective effect.
CN116554346BActive Publication Date: 2026-07-17SHANDONG AGRICULTURAL UNIVERSITY
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- SHANDONG AGRICULTURAL UNIVERSITY
- Filing Date
- 2023-03-28
- Publication Date
- 2026-07-17
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Figure CN116554346B_ABST
Abstract
本发明属于基因工程领域，提供了一种串联羊源大肠杆菌毒力基因融合蛋白的制备和应用，采用预测工具根据分析出的亲水性、疏水性、可塑性、表面活性等指标，成功预测了五种毒力基因的抗原表位，并通过基因工程技术对预测的这五种抗原基因融合并进行串联表达，制备了融合蛋白抗原；同时，将串联羊源大肠杆菌五种毒力基因的融合蛋白制作成亚单位疫苗，取得了良好的免疫保护效果。本发明成功构建了重组羊源大肠杆菌毒力基因原核表达质粒pET28a‑S12345，实现了羊源大肠杆菌在大肠杆菌原核系统上的串联表达，表达产物能够获得较好的免疫保护效果。
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