一种酿酒酵母突变体及其在制备酒精中的应用
By mutating serine at position 485 of the Hsp82 protein in Saccharomyces cerevisiae to alanine, thus preventing its phosphorylation, the negative impact of gene knockout and ATPase overexpression on yeast growth rate in existing technologies was resolved, resulting in a high-efficiency increase in ethanol production.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- BEIJING NORMAL UNIVERSITY
- Filing Date
- 2022-01-28
- Publication Date
- 2026-07-17
AI Technical Summary
Existing methods for increasing ethanol production in Saccharomyces cerevisiae suffer from problems such as the cumbersome gene knockout process, which affects the growth rate of the strain. Furthermore, overexpression of ATPase accelerates intracellular ATP consumption, reducing the ethanol production rate per unit volume.
By mutating serine position 485 of the Hsp82 protein in the Saccharomyces cerevisiae genome to alanine, phosphorylation is prevented, thus forming a Saccharomyces cerevisiae mutant. This avoids the use of ethanol as a carbon source, reduces the oxidative phosphorylation process, and increases ethanol production.
It significantly increases ethanol yield without affecting strain growth and remains stable under both nutrient-sufficient and insufficient conditions, making it suitable for industrial alcohol production.
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