一种外泌体的分离检测方法及应用
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- ZHONGSHAN HOSPITAL FUDAN UNIV
- Filing Date
- 2023-05-08
- Publication Date
- 2026-07-17
AI Technical Summary
Existing liver cancer detection technologies suffer from low sensitivity and poor accuracy. Traditional serological markers such as alpha-fetoprotein (AFP) show low accuracy, and early symptoms of hepatocellular carcinoma are not obvious. Traditional methods such as liver resection and transplantation are limited, necessitating more accurate markers for early diagnosis and monitoring.
Exosomes were separated using magnetic iron oxide nanospheres coated with titanium dioxide nanocoating, and in situ mass spectrometry was performed using MALDI-TOF MS. Significant metabolic biomarkers were screened by utilizing the strong interaction and rapid magnetic response between the titanium dioxide nanocoating and the exosomes.
It enables rapid, sensitive, and selective separation and detection of exosomes, and screens out 6 significantly different metabolic markers, achieving rapid in vitro diagnosis and prognostic monitoring of hepatocellular carcinoma with high sensitivity and accuracy.
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Figure CN116577403B_ABST