Cosmetic compositions and uses thereof

Abstract

The present application discloses a cosmetic composition and its use. The hydroxytyrosol and the acetylated hyaluronic acid or its salt in the composition of the present application have a synergistic effect in promoting the proliferation of keratinocytes, which suggests that the composition can be used to promote the repair of the skin and enhance the barrier function of the skin, which is beneficial for resisting skin aging.

Description

Technical Field

[0001] This application relates to the field of skin care technology, and more particularly to a cosmetic composition and its use. Background Technology

[0002] As we age, our skin is exposed to external factors such as prolonged sun exposure or ultraviolet radiation, microbial invasion, chemical substances, and physical damage. This alters the proliferation and differentiation of keratinocytes, weakening the skin barrier function. With economic development and improved living standards, people are paying increasing attention to skincare, and skin repair is gradually becoming a hot topic in the skincare market. This necessitates the application of more and better active ingredients and compositions with superior repair effects in skin repair products to meet consumer demand.

[0003] While many studies have reported that hydroxytyrosol can increase cell proliferation and has anti-inflammatory effects, reducing the release of inflammatory factors in the skin and helping to repair and improve the skin barrier, more effectively promoting the proliferation of keratinocytes and improving skin repair and barrier function remains a goal pursued in this field. Summary of the Invention

[0004] After in-depth research, the applicant of this application discovered that a combination of acetylated hyaluronic acid or its salt, which has almost no effect on or even inhibits the proliferation of keratinocytes, and hydroxytyrosol produced a synergistic effect in promoting the proliferation of keratinocytes, thus completing this application.

[0005] The specific technical solution of this application is as follows:

[0006] 1. A cosmetic composition comprising acetylated hyaluronic acid or a salt thereof and hydroxytyrosol, wherein the mass ratio of said acetylated hyaluronic acid or a salt thereof to hydroxytyrosol is 300-25:1.

[0007] 2. The composition according to claim 1, wherein the mass ratio of the acetylated hyaluronic acid or its salt to hydroxytyrosol is 260-40:1.

[0008] 3. The composition according to any one of items 1-2, wherein the concentration of the acetylated hyaluronic acid or a salt thereof is 0.001-5 wt%.

[0009] 4. The composition according to any one of items 1-3, wherein the concentration of the hydroxytyrosol is 0.0001-1 wt%.

[0010] 5. Use of the composition of any one of items 1-4 in promoting keratinocyte proliferation.

[0011] 6. Use of the composition of any one of items 1-4 in cosmetics.

[0012] 7. Use of the composition of any one of items 1-4 in enhancing the skin barrier.

[0013] 8. Use of the composition of any one of items 1-4 in promoting skin repair.

[0014] 9. Use of the composition of any one of items 1-4 in combating skin aging.

[0015] The effects of the invention

[0016] The hydroxytyrosol and acetylated hyaluronic acid or their salts in the composition described in this application have a synergistic effect in promoting the proliferation of keratinocytes, suggesting that the composition can be used to promote skin repair and enhance the skin barrier function, which is beneficial for resisting skin aging. Detailed Implementation

[0017] The embodiments described below provide a detailed description of this application. While specific embodiments of this application are shown, it should be understood that this application can be implemented in various forms and should not be limited to the embodiments set forth herein. Rather, these embodiments are provided to enable a more thorough understanding of this application and to fully convey the scope of this application to those skilled in the art.

[0018] It should be noted that certain terms are used in the specification and claims to refer to specific components. Those skilled in the art will understand that different terms may be used to refer to the same component. This specification and claims do not distinguish components based on differences in terminology, but rather on differences in function. The terms "comprising" or "including" used throughout the specification and claims are open-ended and should be interpreted as "comprising but not limited to." The following descriptions in the specification are preferred embodiments for carrying out this application; however, these descriptions are for the purpose of understanding the general principles of the specification and are not intended to limit the scope of this application. The scope of protection of this application shall be determined by the appended claims.

[0019] Composition

[0020] One subject matter of this application relates to compositions, which are defined as long as they achieve the effects of this application. The "composition" includes, but is not limited to, the simultaneous or sequential use of the components. "Simultaneous use" includes using them together in the same formulation or separately in different formulations. "Sequential use" includes using them sequentially in different formulations, with no restriction on the order of sequential use.

[0021] This application provides a cosmetic composition comprising acetylated hyaluronic acid or a salt thereof and hydroxytyrosol, wherein the mass ratio of the acetylated hyaluronic acid or a salt thereof to hydroxytyrosol is 300-25:1. In some embodiments, the mass ratio of the acetylated hyaluronic acid or a salt thereof to hydroxytyrosol is 260-40:1, preferably 200-50:1.

[0022] The acetylated hyaluronic acid or its salt in the composition works synergistically with hydroxytyrosol to significantly increase keratinocyte proliferation, thereby promoting skin barrier repair.

[0023] The mass ratio of the acetylated hyaluronic acid or its salt to hydroxytyrosol (m) 乙酰化透明质酸或其盐 :m 羟基酪醇 The ratios can be 300:1, 290:1, 280:1, 270:1, 260:1, 250:1, 240:1, 230:1, 220:1, 210:1, 200:1, 190:1, 180:1, 170:1, 160:1, 150:1, 140:1, 130:1, 120:1, 110:1, 100:1, 90:1, 80:1, 70:1, 60:1, 50:1, 40:1, 30:1, 25:1, etc.

[0024] In some embodiments, the concentration of the acetylated hyaluronic acid or its salt is 0.001-5 wt%.

[0025] In this application, the concentration of acetylated hyaluronic acid or its salt refers to the amount added to the skin care product. For example, the concentration of acetylated hyaluronic acid or its salt can be 0.001wt%, 0.005wt%, 0.01wt%, 0.05wt%, 0.1wt%, 0.5wt%, 1wt%, 2wt%, 3wt%, 4wt%, 5wt%, etc.

[0026] In this application, the acetylated hyaluronic acid or its salt can be commercially available or prepared in-house. The acetylated hyaluronic acid or its salt is not particularly limited; for example, it can be acetylated hyaluronic acid or its salt with an intrinsic viscosity in the range of 0.1-10.0 dl / g. For instance, the intrinsic viscosity of acetylated hyaluronic acid or its salt can be 0.1 dl / g, 0.2 dl / g, 0.3 dl / g, 0.4 dl / g, 0.5 dl / g, 0.6 dl / g, 0.7 dl / g, 0.8 dl / g, 0.9 dl / g, 1.0 dl / g, 2.0 dl / g, 3.0 dl / g, 4.0 dl / g, 5.0 dl / g, 6.0 dl / g, 7.0 dl / g, 8.0 dl / g, 9.0 dl / g, 10.0 dl / g, etc.

[0027] In some embodiments, the concentration of the hydroxytyrosol is 0.0001-1 wt%.

[0028] In this application, the concentration of hydroxytyrosol refers to the amount of hydroxytyrosol added to the skin care product. For example, the concentration of hydroxytyrosol can be 0.0001wt%, 0.0005wt%, 0.001wt%, 0.005wt%, 0.01wt%, 0.05wt%, 0.1wt%, 0.5wt%, 1wt%, etc.

[0029] In some embodiments, the composition further comprises excipients and / or active ingredients acceptable for skin care products.

[0030] In this application, the excipients and / or active ingredients acceptable for the skin care products are conventional excipients and / or active ingredients in the art. This application does not impose any restrictions, and they can be conventionally selected as needed.

[0031] The composition described in this application may be, for example, one of a skin care composition, a skin repair composition, an anti-aging composition, a skin barrier strengthening composition, etc.

[0032] The composition described in this application can be applied to desired areas of the subject, such as the skin, to enhance the skin barrier or promote skin repair or combat skin aging by promoting the proliferation of keratinocytes.

[0033] The composition described in this application is applied to the desired site on the subject for non-therapeutic purposes, i.e., applied in vitro, thereby enhancing the skin barrier or promoting skin repair or resisting skin aging.

[0034] There are no restrictions on the dosage form of the composition, which can be conventionally selected as needed, such as preparing conventional dosage forms in the art, such as ointments, emulsions, etc.

[0035] In some embodiments, the composition comprises acetylated hyaluronic acid or a salt thereof and hydroxytyrosol, wherein the mass ratio of the acetylated hyaluronic acid or its salt to hydroxytyrosol is 300-25:1. In some embodiments, the mass ratio of the acetylated hyaluronic acid or its salt to hydroxytyrosol is 260-40:1. In some embodiments, the concentration of the acetylated hyaluronic acid or its salt is 0.001-5 wt%. In some embodiments, the concentration of the hydroxytyrosol is 0.0001-1 wt%.

[0036] This application provides the use of the above-described composition in promoting keratinocyte proliferation.

[0037] The experiments presented in this application demonstrate that by combining acetylated hyaluronic acid or its salt with hydroxytyrosol, the two work synergistically to significantly promote the proliferation of keratinocytes.

[0038] This application provides the use of the above-described compositions in cosmetics.

[0039] The compositions described in this application can be used in cosmetics because they can promote the proliferation of keratinocytes, thereby enhancing the skin barrier and / or promoting skin repair and / or resisting skin aging.

[0040] This application provides the use of the above-described composition in enhancing the skin barrier.

[0041] This application also provides the use of the above-described composition in promoting skin repair.

[0042] This application also provides the use of the above-described compositions in combating skin aging.

[0043] Example

[0044] This application provides a general and / or specific description of the materials and methods used in the experiments. In the following examples, unless otherwise specified, % represents wt%, i.e., weight percentage. All reagents and instruments used, unless otherwise specified, are commercially available conventional reagent products. Specifically, KC2500 culture medium was purchased from Guangdong Boxi Biotechnology Co., Ltd., PBS from Solarbio, MTT from Sigma, DMSO from Sigma, EGF from Gibco, acetylated sodium hyaluronate from Bloomage Biotechnology Co., Ltd. (batch number: 22062321), and hydroxytyrosol from Hangzhou Weibaolai Biotechnology Co., Ltd.

[0045] Examples 1-3

[0046] Acetylated sodium hyaluronate and hydroxytyrosol were weighed and dissolved in KC2500 culture medium to obtain the composition.

[0047] Comparative Example 1

[0048] Weigh out hydroxytyrosol and dissolve it in KC2500 culture medium to obtain hydroxytyrosol solution.

[0049] Comparative Examples 2-7

[0050] Weigh out acetylated sodium hyaluronate and dissolve it in KC2500 culture medium to obtain acetylated sodium hyaluronate solution.

[0051] Comparative Examples 8-10

[0052] Acetylated sodium hyaluronate and hydroxytyrosol were weighed and dissolved in KC2500 culture medium to obtain the composition.

[0053] The compositions of Examples 1-3 and Comparative Examples 1-10 are shown in Table 1:

[0054] Table 1

[0055] Control group 1 Blank control - - Comparative Example 1 Hydroxytyrosol 0.003 - Comparative Example 2 Acetylated sodium hyaluronate 0.3 - Comparative Example 3 Acetylated sodium hyaluronate 0.15 - Comparative Example 4 Acetylated sodium hyaluronate 0.06 - Comparative Example 5 Acetylated sodium hyaluronate 0.003 - Comparative Example 6 Acetylated sodium hyaluronate 1.2 - Comparative Example 7 Acetylated sodium hyaluronate 0.6 - Comparative Example 8 Hydroxytyrosol + Acetylated Sodium Hyaluronate 0.003+1.2 1：400 Comparative Example 9 Hydroxytyrosol + Acetylated Sodium Hyaluronate 0.003+0.06 1：20 Comparative Example 10 Hydroxytyrosol + Acetylated Sodium Hyaluronate 0.003+0.003 1：1 Example 1 Hydroxytyrosol + Acetylated Sodium Hyaluronate 0.003+0.3 1：100 Example 2 Hydroxytyrosol + Acetylated Sodium Hyaluronate 0.003+0.15 1：50 Example 3 Hydroxytyrosol + Acetylated Sodium Hyaluronate 0.003+0.6 1：200

[0056] Experimental example:

[0057] (1) Testing system: The cells used in this test are keratinocytes, which were provided by Guangdong Boxi Biotechnology Co., Ltd.

[0058] (2) Main equipment: CO2 incubator (Thermo, 150I), clean bench (Sujing Antai, SW-CJ-1F), microplate reader (BioTek, Epoch)

[0059] (3) Test method:

[0060] a) Cell seeding: at 7 × 10 3 Inoculate the plates at a density of 10 cells / well into 96-well plates and incubate overnight in an incubator (37°C, 5% CO2);

[0061] b) The samples prepared in the above embodiments and comparative examples are used as working solutions of the test substance;

[0062] c) Drug administration: When the cell deposition rate in the 96-well plate reaches 20% to 30%, the cells are administered in groups, with 200 μL added to each well. Each group has 3 replicates. After drug administration, the cells are placed in an incubator (37℃, 5% CO2) for 24 h.

[0063] d) ELISA detection: After the cells were incubated for 24 hours, the supernatant was discarded, MTT working solution was added, and the cells were incubated at 37°C in the dark for 4 hours. After the incubation, the supernatant was discarded, 150 μL of DMSO was added to each well, and the OD value was read at 490 nm.

[0064] e) The readings were based on the blank control group. The proliferation rate was calculated as (relative vigor of the experimental group - relative vigor of the blank control group) / relative vigor of the blank control group. The results are shown in Table 2.

[0065] Table 2

[0066] Control group 1 109.30％ - - Comparative Example 1 117.06％ 7.10％ - Comparative Example 2 112.33％ 2.77％ - Comparative Example 3 107.28％ -1.85％ - Comparative Example 4 108.59％ -0.65％ - Comparative Example 5 107.38％ -1.76％ - Comparative Example 6 107.39％ -1.75％ - Comparative Example 7 107.93％ -1.25％ - Comparative Example 8 107.31％ -1.82％ -0.34 Comparative Example 9 113.65％ 3.98％ 0.61 Comparative Example 10 114.36％ 4.63％ 0.85 Example 1 128.11％ 17.21％ 1.78 Example 2 116.58％ 6.66％ 1.24 Example 3 125.58％ 14.89％ 2.51

[0067] To illustrate the synergistic effect of the composition, the King's formula is used: Q = E(a + b) / (Ea + Eb - Ea × Eb) (where Q < 0.55 indicates significant antagonism, Q = 0.55–0.85 indicates antagonism, Q = 0.85–1.15 indicates additive effect, and Q > 1.15 indicates enhancement).

[0068] Taking Example 1 as an example, Ea is the cell proliferation rate of hydroxytyrosol, which is 7.1%; Eb is the cell proliferation rate of 0.3% acetylated sodium hyaluronate, which is 2.77%; E(a+b) is the cell proliferation rate of the combination of hydroxytyrosol and acetylated sodium hyaluronate, which is 17.21%. Substituting the data into the King's formula, we get the keratinocyte proliferation rate Q of Example 1 as 1.78, indicating that the composition described in Example 1 has a synergistic effect and can significantly increase keratinocyte proliferation, thereby promoting skin barrier repair.

[0069] In summary, the compositions of the embodiments of this application can significantly increase the proliferation of keratinocytes, thereby promoting skin repair, enhancing the skin barrier function, and resisting skin aging.

[0070] The above description is merely a preferred embodiment of this application and is not intended to limit the application in any other way. Any person skilled in the art may make changes or modifications to the disclosed technical content to create equivalent embodiments. However, any simple modifications, equivalent changes, and modifications made to the above embodiments based on the technical essence of this application without departing from the scope of the technical solution of this application shall still fall within the protection scope of this application.

Claims

1. A cosmetic composition comprising acetylated hyaluronic acid or a salt thereof and hydroxytyrosol, wherein, The mass ratio of the acetylated hyaluronic acid or its salt to hydroxytyrosol is 200-50:

1.

2. The composition according to claim 1, wherein, The concentration of the acetylated hyaluronic acid or its salt is 0.001-5 wt%.

3. The composition according to any one of claims 1-2, wherein, The concentration of the hydroxytyrosol is 0.0001-1 wt%.

4. Use of the composition according to any one of claims 1-3 in the preparation of cosmetics that promote keratinocyte proliferation.

5. Use of the composition according to any one of claims 1-3 in the preparation of cosmetics.

6. Use of the composition of any one of claims 1-3 in the preparation of a product that enhances the skin barrier.

7. Use of the composition according to any one of claims 1-3 in the preparation of a product that promotes skin repair.

8. Use of the composition according to any one of claims 1-3 in the preparation of a product for combating skin aging.