Apparatus, method and hydrogel microcapsules for preparing hydrogel microcapsules

Abstract

The application provides a device and method for preparing hydrogel microcapsules, and the hydrogel microcapsules, the device comprising: a propelling motor with a rotating flywheel, a glass slide with a super-biphobic coating, a concentric needle, a microsyringe pump, and a UV light source; under the double action of flywheel rapid rotation and pump propulsion, a multi-layer liquid column generated by the injection of the concentric needle undergoes plato-rayleigh instability when passing through the surface of the super-biphobic coating with extremely low surface energy, and microdroplets are generated; then under the irradiation of the UV light, the hydrogel prepolymer is rapidly crosslinked through a free radical reaction to form hydrogel microcapsules containing a solidified shell. The hydrogel microcapsules prepared by the device and method provided by the application have great application value in cell culture, organoid model construction, cell therapy, drug release, drug screening and other biomedical fields.

Description

Technical Field

[0001] This invention relates to the fields of materials chemistry and biomedicine, and particularly to an apparatus, method, and hydrogel microcapsules for preparing hydrogel microcapsules. Background Technology

[0002] Hydrogels are a class of macromolecular polymers composed of cross-linked polymer molecular chains. Due to their high water content, diverse properties, and similarity to the natural extracellular matrix, they are frequently used for cell culture, tissue regeneration, and delivery of bioactive substances such as drugs, showing broad application prospects in the biomedical field. Hydrogel microcapsules are spherical hydrogels with a core-shell structure, ranging in diameter from a few micrometers to hundreds of micrometers, offering many advantages: 1. Compared to bulk hydrogels, their smaller size allows for injection via small needles, facilitating minimally invasive delivery of cells and drugs; 2. Microspheres of different compositions and sizes can be mixed to construct different materials; 3. The capsule shell provides mechanical stability for the encapsulated cells, drugs, or bioactive substances. Therefore, the preparation methods of hydrogel microcapsules have received increasing attention in recent years.

[0003] Current methods for preparing hydrogel microcapsules often require organic solvents and emulsifiers, which are not green or environmentally friendly. Moreover, collecting the microcapsules requires repeated high-speed centrifugation and cyclic washing, which can lead to microcapsule rupture and low recovery efficiency. Therefore, it is necessary to find a simple, green and environmentally friendly method for preparing hydrogel capsules. Summary of the Invention

[0004] To address the problems in the prior art, this application proposes an apparatus for preparing hydrogel microcapsules. The apparatus includes: a propulsion motor with a rotating flywheel, a glass slide with a superhydrophobic coating, a concentric needle, a micro-injection pump, and an ultraviolet light source. Under the combined action of rapid flywheel rotation and pump propulsion, a multi-layered liquid column injected by the concentric needle undergoes Prato-Rayleigh instability upon passing over the surface of the superhydrophobic coating with extremely low surface energy, generating microdroplets. Then, under ultraviolet light irradiation, the hydrogel prepolymer undergoes rapid cross-linking via free radical reaction to form hydrogel microcapsules containing a solidified shell.

[0005] Preferably, the micro-injection pump includes two syringes with concentric needles having an inner and outer double-layer structure, capable of injecting the generated double-layer liquid column.

[0006] Preferably, the parameters of the inner and outer double-layer concentric axis needle are: inner diameter of the inner needle 170μm, outer diameter of the inner needle 350μm, inner diameter of the outer needle 510μm, outer diameter of the outer needle 810μm; and the wavelength of the ultraviolet light source is 405nm.

[0007] This application relates to a method for preparing hydrogel microcapsules, comprising the following steps:

[0008] Step S1: Synthesize methacrylamide gelatin. Dissolve the synthesized methacrylamide gelatin, polyethylene oxide, and photoinitiator LAP in water to obtain methacrylamide gelatin / polyethylene oxide / LAP mixtures of different concentrations, which are used as shell solutions for later use.

[0009] Step S2: After digestion, one or more cell types are added to a 1:1 volume ratio culture medium and Matrigel. TM The solution was thoroughly mixed to obtain a cell density of 10. 4 -10 9 A cell suspension of 1 cell / mL was prepared as a nuclear layer solution for later use.

[0010] Step S3: Connect the syringe containing the shell solution prepared in step S1 to the outer needle, and connect the syringe containing the core solution prepared in step S2 to the inner needle. Microfibers are formed on the surface of a glass slide with a superhydrophobic coating by an injection pump and a propulsion motor with a rotating flywheel. Subsequently, the microfibers spontaneously break to form droplets with a core-shell structure. The resulting droplets are solidified under an ultraviolet light source to obtain hydrogel microcapsules.

[0011] Preferably, the synthesis step of methacrylamide gelatin in step S1 includes: preparing a gelatin solution with PBS, adding methacrylic anhydride to the prepared gelatin solution, reacting fully, and then adding PBS to terminate the reaction; next, dialyzing with deionized water, filtering the dialysate, and then freeze-drying it in a freeze dryer to obtain methacrylamide gelatin; the gelatin solution concentration is 0.01-0.2 g / mL, the methacrylic anhydride solution concentration is 5%-10%, the mass ratio of gelatin to methacrylic anhydride is 5:4, the volume ratio of PBS added in the two steps is 1:4; the methacrylic anhydride addition rate is 0.5 mL / min; the dialysis time is 1-20 days, the filter pore size is 0.22-0.8 μm, and the freeze-drying time is 1-20 days.

[0012] Preferably, the preparation step of the methacrylamide gelatin / polyethylene oxide / LAP mixture in step S1 includes: dissolving methacrylamide gelatin in PBS containing LAP; filtering the methacrylamide gelatin solution through a 0.22 μm filter membrane before use; adding an appropriate weight of polyethylene oxide powder to the methacrylamide gelatin solution according to the required concentration; then stirring the mixture at 37°C for 3 hours to completely dissolve the polyethylene oxide and form a homogeneous methacrylamide gelatin / polyethylene oxide / LAP mixture; the concentration of methacrylamide gelatin is 5%-20%, the concentration of LAP is 0.1%-1%, and the concentration of polyethylene oxide is 1%-3%.

[0013] Preferably, in step S3, the feed flow rate of the syringe pump is: 90-300 μl / min for the inner needle and 150-500 μl / min for the outer needle; the propulsion speed and motor speed of the propulsion motor with rotating flywheel are 450-500 mm / min and 10-30 rad / s, respectively; and the ultraviolet light intensity is 25 mW / cm². 2 The curing time is 15-25 seconds.

[0014] Preferably, the parameters of the inner and outer double-layer concentric axis needle are: inner diameter of the inner needle 170μm, outer diameter of the inner needle 350μm, inner diameter of the outer needle 510μm, outer diameter of the outer needle 810μm; and the wavelength of the ultraviolet light source is 405nm.

[0015] Preferably, the hydrogel microcapsules are peeled and collected by rolling a 10 μL drop of cell culture medium onto the superhydrophobic surface, and the collected microcapsules can be directly placed into a culture dish for culture.

[0016] This application relates to a hydrogel microcapsule, which is prepared by the apparatus described above for preparing hydrogel microcapsules.

[0017] The above-mentioned technical features can be combined in various suitable ways or replaced by equivalent technical features, as long as the purpose of the present invention can be achieved.

[0018] The apparatus, method, and hydrogel microcapsules provided by this invention have at least the following advantages compared with the prior art:

[0019] 1. The process for preparing hydrogel microcapsules in this invention is simple, has high production efficiency, and is universally applicable. It does not require the use of any additional organic solvents, emulsifiers, etc., making it green and environmentally friendly.

[0020] 2. During preparation, fluid instability, a superhydrophobic coating, and a hydrogel liquid column were combined to achieve rapid and large-scale synthesis of hydrogel microcapsules on a surface with a superhydrophobic coating as the substrate. This avoids the need for repeated high-speed centrifugation and cyclic washing to collect the hydrogel microcapsules, better protects the prepared hydrogel microcapsules, simplifies the production steps, and improves the production efficiency of hydrogel microcapsules.

[0021] 3. By adjusting the feed flow rate of the injection pump, the propulsion speed of the motor in the propulsion motor with the rotating flywheel, and the motor speed, the size, structure, and performance of the hydrogel capsules can be controlled, exhibiting good controllability.

[0022] Therefore, the hydrogel microcapsules prepared using the equipment and methods provided by this invention have great application value in cell culture, organoid model construction, cell therapy, drug sustained release, drug screening and other biomedical fields. Attached Figure Description

[0023] The invention will now be described in more detail with reference to embodiments and the accompanying drawings.

[0024] Figure 1 A schematic diagram of the apparatus for preparing hydrogel microcapsules;

[0025] Figure 2 Scanning electron microscope image of the microstructure of the superhydrophobic coating surface;

[0026] Figure 3 This is a schematic diagram of the Prato-Rayleigh instability process of a solution column on a superhydrophobic coating surface.

[0027] Figure 4 This is a schematic diagram of hydrogel microcapsules;

[0028] Figure 5 Light microscopy image of a hydrogel microcapsule loaded with cells. Detailed Implementation

[0029] The invention will now be further described with reference to the accompanying drawings.

[0030] like Figure 1 As shown, this invention provides an apparatus for preparing hydrogel microcapsules. The apparatus includes: a propulsion motor with a rotating flywheel, a glass slide with a superhydrophobic coating, a concentric needle, a micro-injection pump, and an ultraviolet light source; under the combined action of rapid flywheel rotation and pump propulsion, a multi-layered liquid column is generated by the concentric needle injection, such as... Figure 3 As shown, when passing through a superaphtholytic coating surface with extremely low surface energy, Prato-Rayleigh instability occurs, generating microdroplets; then, under ultraviolet light irradiation, as... Figure 4-5 As shown, the hydrogel prepolymer undergoes rapid cross-linking via free radical reaction to form hydrogel microcapsules with a solidified shell.

[0031] In one embodiment, such as Figure 2 As shown, the specific steps for preparing a glass slide with a superhydrophobic coating in the apparatus for preparing hydrogel microcapsules include: holding and moving the glass slide substrate in the flame of a burning candle for 1-3 minutes to form a uniform black candle smoke layer; then transferring it to a desiccator containing open containers of tetraethoxysilane solution and ammonia solution, respectively; and performing chemical vapor deposition under vacuum for 24-72 hours to obtain silica particles. The obtained silica particles are then calcined at 500-600℃ for 2-4 hours, followed by perfluorination with a fluorinating agent via chemical vapor deposition for 1-3 hours to obtain the superhydrophobic coating.

[0032] In one embodiment, the specific steps for preparing a glass slide with a superhydrophobic coating in the apparatus for preparing hydrogel microcapsules include: holding and moving a glass slide substrate in the flame of a burning candle for 2 minutes to form a uniform black candle smoke layer; then transferring it to a desiccator containing open containers of 4 mL tetraethoxysilane solution and 4 mL ammonia solution, respectively; and performing chemical vapor deposition under vacuum for 24 hours to obtain silica particles. The obtained silica particles are then calcined at 550°C for 3 hours, followed by perfluorination with 200 μL of trichloro(1H,1H,2H,2H-perfluorooctyl)silane via chemical vapor deposition for 2 hours to obtain the superhydrophobic coating.

[0033] In one embodiment, the parameters of the inner and outer double-layer concentric axis needle are: inner needle 28G (inner diameter: 170μm, outer diameter: 350μm), outer needle 21G (inner diameter: 510μm, outer diameter: 810μm); the wavelength of the ultraviolet light source is 405nm.

[0034] This invention provides a method for preparing hydrogel microcapsules, using the above-mentioned apparatus, specifically including the following steps:

[0035] Step (1): Synthesize methacrylamide gelatin. Dissolve the synthesized methacrylamide gelatin, polyethylene oxide, and photoinitiator LAP in water to obtain methacrylamide gelatin / polyethylene oxide / LAP mixtures of different concentrations, which are used as shell solutions for later use.

[0036] Further, the synthesis steps of methacrylamide gelatin in step (1) include: preparing a gelatin solution with PBS, adding methacrylic anhydride to the prepared gelatin solution, and terminating the reaction by adding PBS after the reaction is complete. Next, the gelatin solution is dialyzed with deionized water, filtered, and then freeze-dried in a freeze dryer to obtain methacrylamide gelatin. The gelatin solution concentration is 0.01-0.2 g / mL, the methacrylic anhydride solution concentration is 5%-10%, the mass ratio of gelatin to methacrylic anhydride is 5:4, and the volume ratio of PBS added in the two steps is 1:4. The methacrylic anhydride addition rate is 0.5 mL / min. The dialysis time is 1-20 days, the filter pore size is 0.22-0.8 μm, and the freeze-drying time is 1-20 days.

[0037] Further, the preparation steps of the methacrylamide gelatin / polyethylene oxide / LAP mixture in step (1) include: dissolving methacrylamide gelatin in PBS containing LAP. The methacrylamide gelatin solution is filtered through a 0.22 μm filter membrane before use. Depending on the desired concentration, an appropriate weight of polyethylene oxide powder is added to the methacrylamide gelatin solution. Then, the mixture is stirred at 37°C for 3 hours to completely dissolve the polyethylene oxide, forming a homogeneous methacrylamide gelatin / polyethylene oxide / LAP mixture. The concentration of methacrylamide gelatin is 5%-20%, the concentration of LAP is 0.1%-1%, and the concentration of polyethylene oxide is 1%-3%.

[0038] Adding polyethylene oxide to methacrylamide gelatin can increase the viscosity of the mixture, which is beneficial for the mixture to form liquid columns on the superhydrophobic surface, thereby breaking them into droplets with good forming properties.

[0039] Step (2): After digestion, one or more cell types are added to a 1:1 volume ratio culture medium and mixed thoroughly with Matrigel™ solution to obtain a cell density of 102. 4 -10 9 A cell suspension of 1 cell / mL was prepared as a nuclear layer solution for later use.

[0040] Step (3): Connect the syringe containing the shell solution prepared in step (1) to the outer needle, and connect the syringe containing the core solution prepared in step (2) to the inner needle. Use an injection pump and a propulsion motor with a rotating flywheel to form microfibers on the surface of a glass slide with a superhydrophobic coating. Subsequently, the microfibers spontaneously break to form droplets with a core-shell structure. The resulting droplets are solidified under an ultraviolet light source to obtain hydrogel microcapsules.

[0041] Furthermore, in step (3), the feed flow rate of the injection pump is: 90-300 μl / min for the inner needle and 150-500 μl / min for the outer needle; the propulsion speed and motor speed of the propulsion motor with rotating flywheel are 450-500 mm / min and 10-30 rad / s, respectively; the ultraviolet light intensity is 25 mW / cm2 and the curing time is 15-25 s.

[0042] In one embodiment, a method for preparing hydrogel microcapsules, using the above-described apparatus, specifically includes the following steps:

[0043] Step (1): Synthesize methacrylamide gelatin. Dissolve the synthesized methacrylamide gelatin, polyethylene oxide, and photoinitiator LAP in water to obtain methacrylamide gelatin / polyethylene oxide / LAP mixtures of different concentrations, which are used as shell solutions for later use.

[0044] The synthesis steps of methacrylamide gelatin include: preparing a 0.2 g / mL gelatin solution with PBS; adding 5% methacrylic anhydride to the prepared gelatin solution at a rate of 0.5 mL / min, with a gelatin to methacrylic anhydride mass ratio of 5:4; and stopping the reaction by adding 4 times the volume of PBS after the reaction has been fully completed. Next, the solution is dialyzed with deionized water for 10 days, filtered through a 0.45 μm filter for 10 days, and then freeze-dried for 3 days to obtain methacrylamide gelatin.

[0045] The methacrylamide gelatin was then dissolved in PBS containing 0.5% LAP. The methacrylamide gelatin solution was filtered through a 0.22 μm filter before use. Polyethylene oxide powder was added to the methacrylamide gelatin solution. The mixture was then stirred at 37°C for 3 hours to completely dissolve the polyethylene oxide, forming a homogeneous methacrylamide gelatin / polyethylene oxide / LAP mixture. The concentration of methacrylamide gelatin was 5%, and the concentration of polyethylene oxide was 2%.

[0046] Step (2): After digestion, MC3T3-E1 cells were added to a 1:1 volume ratio of cell culture medium and mixed thoroughly with Matrigel™ solution to obtain a cell density of 102. 6 A cell suspension of 1 cell / mL was prepared as a nuclear layer solution for later use.

[0047] Step (3): The superhydrophobic glass slide is clamped on a flywheel controlled by a propulsion motor. A syringe containing the shell solution prepared in step (1) is connected to the outer needle, and a syringe containing the core solution prepared in step (2) is connected to the inner needle. The flow rate of the micro-injection pump connected to the inner needle is adjusted to 200 μl / min, and the flow rate of the micro-injection pump connected to the outer needle is adjusted to 400 μl / min. The bilayer liquid column (core layer: cell / Matrigel™, shell layer: methacrylamide gelatin / polyoxyethylene) generated by the concentric needle pushes through the superhydrophobic surface with extremely low surface energy, and the Prato-Rayleigh instability occurs to generate droplets with a core-shell structure. The propulsion speed and motor speed of the stepper motor are 500 mm / min and 20 rad / s, respectively. The resulting droplets are cured for 20 s under a UV light source with an intensity of 25 mW / cm2 to obtain hydrogel microcapsules.

[0048] Step (4): The hydrogel microcapsules were peeled and collected by rolling a 10 μL drop of cell culture medium onto the superhydrophobic surface. The collected microcapsules could then be directly placed into a culture dish for incubation. The average diameter of the obtained microcapsules was 186 μm.

[0049] In one embodiment, the present invention provides an apparatus for preparing hydrogel microcapsules, such as... Figure 1As shown, it mainly consists of a microfluidic rotating device, a glass slide with a superhydrophobic coating, an inner and outer double-layer concentric needle, a micro-injection pump, and an ultraviolet light source.

[0050] The specific steps for preparing the superhydrophobic coating on the glass slide in the apparatus for preparing hydrogel microcapsules include: holding and moving the glass slide substrate in the flame of a burning candle for 2 minutes to form a uniform black candle smoke layer; then transferring it to a desiccator containing open containers of 4 mL tetraethoxysilane solution and 4 mL ammonia solution, respectively; and performing chemical vapor deposition under vacuum for 24 hours to obtain silica particles. The obtained silica particles are then calcined at 550°C for 3 hours, followed by perfluorination with 200 μL of trichloro(1H,1H,2H,2H-perfluorooctyl)silane for 2 hours to obtain the superhydrophobic coating.

[0051] In the device for preparing hydrogel microcapsules, the parameters of the inner and outer double-layer concentric needles are: inner needle 28G (inner diameter: 170μm, outer diameter: 350μm), outer needle 21G (inner diameter: 510μm, outer diameter: 810μm); the wavelength of the ultraviolet light source is 405nm.

[0052] This invention provides a method for preparing hydrogel microcapsules, using the above-mentioned apparatus, specifically including the following steps:

[0053] Step (1): Synthesize methacrylamide gelatin. Dissolve the synthesized methacrylamide gelatin, polyethylene oxide, and photoinitiator LAP in water to obtain methacrylamide gelatin / polyethylene oxide / LAP mixtures of different concentrations, which are used as shell solutions for later use.

[0054] The synthesis steps of methacrylamide gelatin include: preparing a 0.2 g / mL gelatin solution with PBS; adding 5% methacrylic anhydride to the prepared gelatin solution at a rate of 0.5 mL / min, with a gelatin to methacrylic anhydride mass ratio of 5:4; and stopping the reaction by adding 4 times the volume of PBS after the reaction has been fully completed. Next, the solution is dialyzed with deionized water for 10 days, filtered through a 0.45 μm filter for 10 days, and then freeze-dried for 3 days to obtain methacrylamide gelatin.

[0055] The methacrylamide gelatin was then dissolved in PBS containing 0.5% LAP. The methacrylamide gelatin solution was filtered through a 0.22 μm filter before use. Polyethylene oxide powder was added to the methacrylamide gelatin solution. The mixture was then stirred at 37°C for 3 hours to completely dissolve the polyethylene oxide, forming a homogeneous methacrylamide gelatin / polyethylene oxide / LAP mixture. The concentration of methacrylamide gelatin was 5%, and the concentration of polyethylene oxide was 2%.

[0056] Step (2): After digestion, MC3T3-E1 and HUVEC cells were added to a 1:1 volume ratio of cell culture medium and mixed thoroughly with Matrigel™ solution to obtain a cell density of 102. 6 A cell suspension of 1 cell / mL was prepared as a nuclear layer solution for later use.

[0057] Step (3): The superhydrophobic glass slide is clamped on a flywheel controlled by a propulsion motor. The syringe containing the shell solution prepared in step (1) is connected to the outer needle, and the syringe containing the core solution prepared in step (2) is connected to the inner needle. The feed flow rate of the micro-injection pump connected to the inner needle is adjusted to 150 μl / min, and the feed flow rate of the micro-injection pump connected to the outer needle is adjusted to 300 μl / min. The double-layer liquid column (core layer: cell / Matrigel™, shell layer: methacrylamide gelatin / polyoxyethylene) generated by the concentric needle pushes the liquid. When it passes through the superhydrophobic surface with extremely low surface energy, the Prato-Rayleigh instability occurs, generating droplets with a core-shell structure. The propulsion speed and motor speed of the stepper motor are 500 mm / min and 10 rad / s, respectively. The resulting droplets have an intensity of 25 mW / cm. 2 Hydrogel microcapsules were obtained by curing under ultraviolet light for 20 seconds.

[0058] Step (4): The hydrogel microcapsules were peeled and collected by rolling a 10 μL drop of cell culture medium onto the superhydrophobic surface. The collected microcapsules could then be directly placed into a culture dish for incubation. The average diameter of the obtained microcapsules was 250 μm.

[0059] This invention provides a hydrogel microcapsule, which is prepared by the above-described apparatus and method and has a cell / Matrigel structure. TM The hydrogel microcapsules have a core layer and a methacrylamide gelatin shell layer, and the size of the hydrogel microcapsules is uniform and controllable, with an average diameter of 50-1000 μm.

[0060] While the invention has been described herein with reference to specific embodiments, it should be understood that these embodiments are merely examples of the principles and applications of the invention. Therefore, it should be understood that many modifications can be made to the exemplary embodiments, and other arrangements can be designed without departing from the spirit and scope of the invention as defined by the appended claims. It should be understood that different dependent claims and features described herein can be combined in ways different from those described in the original claims. It is also understood that features described in conjunction with individual embodiments can be used in other described embodiments.

Claims

1. A method for preparing hydrogel microcapsules, characterized in that, Includes the following steps: Step S1: Synthesize methacrylamide gelatin. Dissolve the synthesized methacrylamide gelatin, polyethylene oxide, and photoinitiator LAP in water to obtain methacrylamide gelatin / polyethylene oxide / LAP mixtures of different concentrations, which are used as shell solutions for later use. The concentration of methacrylamide gelatin is 5%-20%, the concentration of LAP is 0.1%-1%, and the concentration of polyethylene oxide is 1%-3%. Step S2: After digestion, one or more cell types are added to a 1:1 volume ratio culture medium and Matrigel. TM The solution was thoroughly mixed to obtain a cell density of 10. 4 -10 9 A cell suspension of 1 cell / mL was prepared as a nuclear layer solution for later use. Step S3: Connect the syringe containing the shell solution prepared in step S1 to the outer needle of the concentric needle, and connect the syringe containing the core solution prepared in step S2 to the inner needle of the concentric needle. Use a micro-injection pump and a propulsion motor with a rotating flywheel to form microfibers on the surface of a glass slide with a superhydrophobic coating. Subsequently, the microfibers spontaneously break to form droplets with a core-shell structure. The resulting droplets are solidified under an ultraviolet light source to obtain hydrogel microcapsules. In step S3, the feed flow rate of the syringe pump is: 90-300 μl / min for the inner needle and 150-500 μl / min for the outer needle; the propulsion speed and motor speed of the propulsion motor with rotating flywheel are 450-500 mm / min and 10-30 rad / s, respectively; the ultraviolet light intensity is 25 mW / cm². 2 The curing time is 15-25 seconds.

2. The method for preparing hydrogel microcapsules according to claim 1, characterized in that, The synthesis steps of methacrylamide gelatin in step S1 include: preparing a gelatin solution with PBS, adding methacrylic anhydride to the prepared gelatin solution, and terminating the reaction by adding PBS after the reaction is complete; then dialyzing with deionized water, filtering the dialysate, and lyophilizing it in a freeze dryer to obtain methacrylamide gelatin; the gelatin solution concentration is 0.01-0.2 g / mL, the methacrylic anhydride solution concentration is 5%-10%, the mass ratio of gelatin to methacrylic anhydride is 5:4, the volume ratio of PBS added in the two steps is 1:4, the methacrylic anhydride addition rate is 0.5 mL / min, the dialysis time is 1-20 days, the filter pore size is 0.22-0.8 μm, and the lyophilization time is 1-20 days.

3. The method for preparing hydrogel microcapsules according to claim 1, characterized in that, The preparation steps of the methacrylated gelatin / polyoxyethylene / LAP mixture in step S1 include: dissolving methacrylated gelatin in PBS containing LAP; filtering the methacrylated gelatin solution through a 0.22 μm filter membrane before use; adding the appropriate weight of polyethylene oxide powder to the methacrylated gelatin solution according to the required concentration; and then stirring the mixture at 37°C for 3 hours to completely dissolve the polyethylene oxide and form a homogeneous methacrylated gelatin / polyoxyethylene / LAP mixture.

4. The method for preparing hydrogel microcapsules according to claim 1, characterized in that, The parameters of the inner and outer double-layer concentric axis needle are as follows: inner needle inner diameter 170μm, inner needle outer diameter 350μm, outer needle inner diameter 510μm, outer needle outer diameter 810μm; the wavelength of the ultraviolet light source is 405nm.

5. The method for preparing hydrogel microcapsules according to claim 1, characterized in that, Hydrogel microcapsules were peeled off and collected by rolling a 10 μL drop of cell culture medium onto the superhydrophobic surface, and the collected microcapsules were directly placed into a culture dish for culture.

6. A hydrogel microcapsule, characterized in that, The hydrogel microcapsules are obtained by the method for preparing hydrogel microcapsules as described in claim 1.

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