一种羟胺苯变位酶突变体及其应用
By mutating hydroxylamine phenyl mutase at specific sites, its catalytic activity was improved, solving the problem of low catalytic activity in existing technologies and achieving an increase in the biosynthesis efficiency of 3-amino-2-hydroxyacetophenone.
CN116622690BActive Publication Date: 2026-07-17ZHEJIANG UNIV OF TECH
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- ZHEJIANG UNIV OF TECH
- Filing Date
- 2023-05-10
- Publication Date
- 2026-07-17
AI Technical Summary
Technical Problem
The existing hydroxylamine phenyl mutase has low catalytic activity, resulting in low biosynthetic yield of 3-amino-2-hydroxyacetophenone.
Method used
By mutating the wild-type hydroxylamine phenylmutase from Pseudomonas oleovorans at positions 115, 93, and 81, mutant hydroxylamine phenylmutases were obtained and their catalytic activity was improved.
Benefits of technology
The mutant exhibits significantly enhanced catalytic activity, increasing from a maximum of 0.176 U to 1.40 U, thereby substantially improving the biosynthetic efficiency of 3-amino-2-hydroxyacetophenone.
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Abstract
本发明涉及生物技术领域，本发明公开了一种羟胺苯变位酶突变体及其应用。本发明通过将来源于Pseudomonas oleovorans的野生型羟胺苯变位酶序列进行第115位、93和81位的突变，筛选得到羟胺苯变位酶突变体，突变体在催化3‑羟基氨基乙酰苯转化为3‑氨基‑2‑羟基苯乙酮的过程中，相较于野生型酶活力大大提高，最大可从0.176U提高至1.40U。本发明提供羟胺苯变位酶突变体在3‑氨基‑2‑羟基苯乙酮的生物合成中具有较大工业前景。
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