Nucleic acid extraction consumable

Abstract

The application relates to the technical field of detecting consumables, and discloses a nucleic acid extraction consumable, which comprises a main body provided with a plurality of liquid cavities arranged side by side, and an upper shell and a lower shell arranged on the outer side of the main body; a top cover sealing gasket is arranged between the upper shell and the top end surface of the main body; and a piston assembly is further arranged on the main body; the nucleic acid extraction consumable further comprises a magnetic sleeve, the magnetic sleeve is movably arranged in the liquid cavity, a connecting portion is arranged on the magnetic sleeve, an inner cavity is arranged in the magnetic sleeve for inserting a magnetic rod of an extraction device, and a sealing structure is further arranged on the magnetic sleeve and in sealing cooperation with the top cover sealing gasket. When the magnetic beads in the extraction liquid are extracted, the magnetic rod is inserted into the inner cavity of the magnetic sleeve, the extraction device is connected with the connecting portion of the magnetic sleeve and drives the magnetic sleeve to move axially in the magnetic bead cavity to a communication state, the sealing structure of the magnetic sleeve is separated from the top cover sealing gasket, the liquid cavity is in communication with the atmospheric pressure outside, the magnetic rod of the extraction device can rotate in the magnetic sleeve, the liquid in the magnetic bead cavity is in a vortex state, and therefore the magnetic beads can be cleaned more effectively, and the cleaning efficiency of the magnetic beads is improved.

Description

Technical Field

[0001] This invention relates to the field of testing consumables technology, and in particular to a nucleic acid extraction consumable. Background Technology

[0002] Nucleic acids are biological macromolecules composed of many nucleotide monomers, and are one of the most basic substances of life. Nucleic acids combine with protein to form nucleosomal proteins. Different nucleic acids have different chemical compositions and nucleotide sequences. In medicine, detecting the sequence of nucleic acids allows for advanced biological detection at the molecular level, enabling the identification of the type of virus causing the infection. Nucleic acid extraction involves processes such as extraction and washing.

[0003] For example, Chinese patent CN214937394U discloses an automated nucleic acid extractor, including a housing and a rectangular block. A base plate is fixedly connected to the inner bottom wall of the housing. A limiting groove is formed on the top of the base plate. A cylinder is fixedly connected to the top of the base plate. A support spring is fixedly connected to the inner surface of the cylinder. A semi-ring block is fixedly connected to one end of the support spring. A pad is fixedly connected to the inner surface of the semi-ring block. A pull rod is fixedly connected to the outer surface of the semi-ring block. A cross bar is fixedly connected to the front of the pull rod. A partition is fixedly connected to the inner side wall of the rectangular block. A first servo motor is fixedly connected to the top of the partition. A ring block is rotatably connected to the inside of the partition via bearings. A toothed ring is fixedly connected to the top of the ring block. The toothed ring meshes with a drive gear. A stirring rod is fixedly connected to the bottom of the ring block. A permanent magnet is fixedly connected to the inner top wall of the rectangular block. This automated nucleic acid extractor uses a cylinder to fix test tubes and a permanent magnet to attract magnetic beads for nucleic acid detection.

[0004] However, the above-mentioned detection is applicable to samples containing nucleic acid obtained after a series of lysis and amplification processes on the collected nucleic acid samples, requiring the tester to place the test tube inside the cylinder. With the popularization of automated testing, integrated sample reaction consumables are now widely used. For example, patent application publication number CN105695303A discloses a sample reaction device, including a piston cylinder, comprising a cylinder body, a piston, a piston rod, and a base plate that seals the bottom of the cylinder body; the cross-sectional area of ​​the base plate is larger than the cross-sectional area of ​​the cylinder body; the base plate has a first through hole communicating with the cylinder body, and a second through hole and a third through hole located outside the connection between the cylinder body and the base plate, penetrating the upper and lower surfaces of the base plate, and whose centers are not on the same radius extension line; the annular zone where the longest and shortest radii of the second through hole are located from the center of the base plate is not... There is an intersection; a base, fixedly connected to the chassis of the piston cylinder, the base body is provided with a first flow channel connecting the first through hole and the second through hole, and a second flow channel connecting the first through hole, the third through hole and the test area; a container part that can rotate relative to the cylinder body includes multiple chambers circumferentially arranged around the piston cylinder, each chamber having a connecting hole at the bottom and a sealing device at the top; one of the multiple chambers, after the container part rotates to a set angle, its connecting hole at the bottom aligns with the third through hole and connects with the second flow channel and the cylinder body; the remaining chambers of the multiple chambers, after the container part rotates by a predetermined angle, their connecting holes at the bottom align with the second through hole and connect with the cylinder body through the first flow channel.

[0005] The cylinder and base of the above-mentioned sample reaction device can rotate relative to each other. When the base rotates, it drives the chassis on it to rotate. Multiple through holes on the chassis form a channel connecting each chamber. By pulling the piston rod, the liquid in the chamber is moved, realizing the automatic transfer of liquid samples.

[0006] However, the aforementioned sample reaction apparatus primarily performs sequential liquid reactions. During magnetic bead extraction, the magnetic sleeve of the extraction device extends into the corresponding chamber. Magnetic bead elution is the most challenging issue for nucleic acid consumables. In the elution process, to improve adsorption efficiency, operations such as liquid stirring are required. Most designs on the market rely on liquid flushing for cleaning, resulting in low elution efficiency. Summary of the Invention

[0007] The purpose of this invention is to provide a nucleic acid extraction consumable to solve the problem of low efficiency in the existing sample reaction device when cleaning with liquid pressure during magnetic bead elution.

[0008] To achieve the above objectives, the present invention provides a nucleic acid extraction consumable, comprising a main body having a plurality of liquid chambers arranged in parallel, wherein the liquid chambers include magnetic bead chambers for magnetic bead elution, an upper shell and a lower shell are fitted on the outer side of the main body, a top cover sealing gasket is arranged between the upper shell and the top surface of the main body, and a piston assembly for transferring liquid in each of the liquid chambers is also arranged on the main body.

[0009] It also includes a magnetic sleeve, which is movably arranged within the magnetic bead cavity. The magnetic sleeve is provided with a connecting part for transmission connection with the extraction device to drive the magnetic sleeve to move axially. The magnetic sleeve is provided with an inner cavity for the magnetic rod of the extraction device to be inserted and rotated. The magnetic sleeve is also provided with a sealing structure. During the axial movement stroke, the magnetic sleeve has a sealed state in which the sealing structure and the top cover sealing gasket seal to close the liquid cavity when it is inserted into the liquid cavity, and a connected state in which the sealing structure and the top cover sealing gasket separate to allow the liquid cavity to communicate with the outside.

[0010] Preferably, the sealing structure is a sealing groove arranged on the outside of the magnetic sleeve, and the top cover sealing gasket is interference-fitted with the sealing groove.

[0011] Preferably, the outer side of the magnetic sleeve is provided with a flange, which is axially fitted to the upper outer shell along the direction in which the magnetic sleeve is inserted into the liquid cavity, and the distance between the flange and the sealing groove is equal to the distance between the upper outer shell and the sealing gasket.

[0012] Preferably, the magnetic sleeve is further provided with a boss, the boss and the flange are arranged at intervals along the axial direction of the magnetic sleeve, the sealing groove is arranged on the boss, the upper outer shell is provided with an inwardly folded flange, and the boss and the flange are both unidirectionally stopped and assembled with the flange.

[0013] Preferably, a guide ramp is provided at the end of the boss away from the flange, and the outer diameter of the guide ramp gradually decreases in the direction away from the boss.

[0014] Preferably, there is a radial gap between the outer wall of the boss and the inner wall of the upper shell, the portion between the boss and the flange is defined as a transition section, and there is a radial gap between the inner wall of the flange and the transition section.

[0015] Preferably, the axial distance between the boss and the flange is 4 mm.

[0016] Preferably, the connecting portion includes connecting claws disposed at the tail of the magnetic sleeve, with each connecting claw arranged at intervals along the circumference of the magnetic sleeve, and an expansion groove for radial expansion and contraction formed between two adjacent connecting claws.

[0017] Preferably, the tail end of the connecting claw is further provided with a stop that blocks the extraction device axially.

[0018] Preferably, the piston assembly includes a sleeve, a piston rod axially movably mounted within the sleeve, and a base fixedly connected to the bottom end of the sleeve. The base is rotatably mounted on the main body, and the base has a pipetting orifice for communicating with one of the liquid chambers during the rotation stroke, and a pipetting channel connecting the sleeve and the pipetting orifice.

[0019] Compared with existing technologies, the nucleic acid extraction consumable of this invention has the following advantages: A magnetic sleeve is movably arranged within the magnetic bead cavity of the main body. When the external extraction device elutes the magnetic beads in the magnetic bead cavity, a magnetic rod is inserted into the inner cavity of the magnetic sleeve. The connection between the extraction device and the magnetic sleeve is established, driving the magnetic sleeve to move axially within the magnetic bead cavity to a connected state. The sealing structure of the magnetic sleeve separates from the top cover sealing gasket, and the liquid cavity is connected to the external atmospheric pressure. The magnetic rod of the extraction device can rotate within the magnetic sleeve, causing the liquid in the magnetic bead cavity to form a vortex state, thereby more effectively cleaning the magnetic beads and improving the cleaning efficiency. After cleaning, the extraction device drives the magnetic sleeve to move axially within the magnetic bead cavity to a sealed state. The sealing structure and the top cover sealing gasket seal together, forming a sealed space between the sealing structure of the magnetic sleeve, the top cover sealing gasket, and the liquid cavity, completing the subsequent detection work. Attached Figure Description

[0020] Figure 1 This is a schematic diagram of the structure of the nucleic acid extraction consumable of the present invention;

[0021] Figure 2 yes Figure 1 An exploded view of the assembly of nucleic acid extraction consumables and magnetic rods;

[0022] Figure 3 This is a cross-sectional view of the nucleic acid extraction consumables and magnetic rods of the present invention after assembly.

[0023] Figure 4 yes Figure 3 An enlarged view of point A;

[0024] Figure 5 This is a schematic diagram of the structure of the magnetic sleeve and the top cover sealing gasket of the nucleic acid extraction consumable of the present invention when they are sealed together.

[0025] Figure 6 This is a schematic diagram of the structure of the nucleic acid extraction consumable of the present invention when the liquid chamber is connected to the outside after the magnetic sleeve is removed;

[0026] Figure 7 This is a schematic diagram showing the state of the nucleic acid extraction consumables and extraction equipment of the present invention when connected.

[0027] In the diagram, 1. Main body, 11. Liquid cavity, 2. Upper outer shell, 21. Flanged edge, 3. Lower outer shell, 4. Top cover sealing gasket, 5. Piston assembly, 51. Sleeve, 52. Piston rod, 53. Base, 6. Magnetic sleeve, 61. Sealing groove, 62. Inner cavity, 63. Flange, 64. Boss, 65. Guide slope, 66. Connecting claw, 67. Baffle, 7. Extraction device, 8. Magnetic rod. Detailed Implementation

[0028] The specific embodiments of the present invention will be described in further detail below with reference to the accompanying drawings and examples. The following examples are for illustrative purposes only and are not intended to limit the scope of the invention.

[0029] A preferred embodiment of a nucleic acid extraction consumable of the present invention, such as... Figures 1 to 7 As shown, the nucleic acid extraction consumable includes a main body 1, an upper outer shell 2, a lower outer shell 3, a top cover sealing gasket 4, a piston assembly 5, and a magnetic sleeve 6. The main body 1 is the structural basis of the extraction consumable, and the upper outer shell 2, the lower outer shell 3, the top cover sealing gasket 4, the piston assembly 5, and the magnetic sleeve 6 are all arranged on the main body 1.

[0030] The main body 1 has several liquid chambers 11 formed within it. These chambers extend vertically along the main body 1 and are arranged side-by-side, with each chamber separated from the others. Each chamber 11 is used to pre-store different types of reagents, allowing for automated processing after the consumables obtain samples. A piston assembly 5 is used to transfer the liquid within each chamber 11. During use, the piston assembly 5 can be connected to one of the chambers 11 to transfer the liquid. The piston assembly 5 can employ an existing structure, the specific structure of which is not described in detail in this embodiment.

[0031] The upper outer shell 2 is fitted onto the upper side of the main body 1, and the lower outer shell 3 is fitted onto the lower side of the main body 1. The upper outer shell 2 covers the openings of each liquid cavity 11. A conical locking platform is provided on the side of the main body 1, and locking slots are provided on both the upper outer shell 2 and the lower outer shell 3. The upper outer shell 2 and the lower outer shell 3 are connected to the main body 1 by locking platforms and locking slots, which can reduce the problem of poor airtightness caused by errors between the main body 1 and the top cover sealing gasket 4 due to injection molding and other processes.

[0032] The top cover sealing gasket 4 is arranged between the top surface of the main body 1 and the upper outer shell 2. The top cover sealing gasket 4 seals the openings of each liquid chamber 11 and the gap between the main body 1 and the upper outer shell 2 to prevent liquid leakage and cross-contamination. The top cover sealing gasket 4 is provided with an opening to avoid the magnetic sleeve 6, which can be inserted into the liquid chamber 11 through the opening.

[0033] The multiple liquid chambers 11 include a magnetic bead chamber for magnetic bead elution. The magnetic sleeve 6 can be movably arranged within the magnetic bead chamber; that is, the liquid chamber 11 into which the magnetic sleeve 6 is placed is the magnetic bead chamber. The opening on the top cover sealing gasket 4 is located at a position corresponding to the opening of the extraction chamber, allowing the magnetic sleeve 6 to pass through. The magnetic sleeve 6 is provided with a connecting part for transmission connection with the extraction device 7. The extraction device 7 can drive the magnetic sleeve 6 to move axially through the connecting part.

[0034] The magnetic sleeve 6 has a hollow structure. Its front end is inserted into the liquid cavity 11, and its tail end is located outside the liquid cavity 11. The connecting part is located at the tail end for easy connection to the extraction device 7. The inner end of the magnetic sleeve 6 is a closed structure, and the magnetic sleeve 6 itself is hollow, forming an inner cavity 62 for the magnetic rod 8 of the extraction device 7 to insert into. The front end of the magnetic rod 8 is equipped with a magnetic component, which can extract and adsorb magnetic beads in the liquid cavity 11. The magnetic rod 8 can rotate within the magnetic sleeve 6, causing the liquid in the magnetic bead cavity to form a vortex state, thereby more effectively cleaning the magnetic beads, improving the extraction efficiency of the magnetic beads, and enhancing the mixing and magnetic separation effect. The specific structure of the magnetic rod 8 is existing technology and will not be described in detail here.

[0035] The magnetic sleeve 6 is also equipped with a sealing structure. During its axial movement within the liquid chamber 11, when the magnetic sleeve 6 is inserted into the liquid chamber 11, the sealing structure and the top cover sealing gasket 4 seal the gap between the magnetic sleeve 6 and the liquid chamber 11, forming a closed space. At this time, the magnetic sleeve 6 is in a sealed state, and the liquid chamber is closed. When eluting the magnetic beads, the magnetic sleeve 6 is pulled outward, the sealing structure separates from the top cover sealing gasket 4, and the magnetic bead cavity is connected to the external atmospheric pressure. At this point, the magnetic sleeve 6 is in a connected state.

[0036] When the external extraction device 7 extracts and washes the magnetic beads in the magnetic bead cavity, the magnetic rod 8 is inserted into the inner cavity 62 of the magnetic sleeve 6. The extraction device 7 connects with the magnetic sleeve 6 and drives the magnetic sleeve 6 to move axially in the magnetic bead cavity to a connected state. The sealing structure of the magnetic sleeve 6 separates from the top cover sealing gasket 4, and the liquid cavity 11 is connected to the external atmospheric pressure. The magnetic rod 8 of the extraction device 7 can rotate in the magnetic sleeve 6, so that the liquid in the magnetic bead cavity forms a vortex state, which can more effectively clean the magnetic beads and improve the cleaning efficiency of the magnetic beads. After cleaning, the extraction device 7 drives the magnetic sleeve 6 to move axially in the magnetic bead cavity to a sealed state. The sealing structure and the top cover sealing gasket 4 seal together, and a sealed space is formed between the sealing structure of the magnetic sleeve 6, the top cover sealing gasket 4, and the liquid cavity 11 to complete the subsequent testing work.

[0037] Preferably, the sealing structure is a sealing groove 61 arranged on the outside of the magnetic sleeve 6, and the top cover sealing gasket 4 is interference-fitted with the sealing groove 61.

[0038] The sealing groove 61 forms a sealing structure, which simplifies the molding process of the sealing structure and facilitates the molding and manufacturing of the magnetic sleeve 6. The top cover sealing gasket 4 and the sealing groove 61 are interference-fitted, which ensures that the two always maintain a seal and helps prevent waste liquid from flowing out.

[0039] Preferably, the outer side of the magnetic sleeve 6 is provided with a flange 63, which is axially opposed to the upper outer shell 2 along the direction in which the magnetic sleeve 6 is inserted into the liquid cavity 11. The distance between the flange 63 and the sealing groove 61 is equal to the distance between the upper outer shell 2 and the sealing gasket.

[0040] The flange 63 is axially stopped and assembled with the outer wall of the upper housing 2. When the external extraction device 7 drives the magnetic sleeve 6 to be inserted into the liquid chamber 11, the upper housing 2 can limit the position of the magnetic sleeve 6. Since the distance between the flange 63 and the sealing groove 61 is equal to the distance between the upper housing 2 and the sealing gasket, when the flange 63 contacts the outer wall of the upper housing 2, the sealing groove 61 on the magnetic sleeve 6 is exactly in sealing fit with the top cover sealing gasket 4 to ensure a seal.

[0041] Preferably, the magnetic sleeve 6 is further provided with a boss 64, the boss 64 and the flange 63 are arranged at intervals along the axial direction of the magnetic sleeve 6, the sealing groove 61 is arranged on the boss 64, and the upper outer shell 2 is provided with an inwardly folded flange 21, and the boss 64 and the flange 63 are both unidirectionally stopped and assembled with the flange 21.

[0042] The sealing groove 61 is arranged on the boss 64. The boss 64 can cooperate with the flange 21 to unidirectionally stop the magnetic sleeve 6. The boss 64 and the flange 63 are located on both sides of the flange 21 of the upper shell 2. The boss 64 and the flange 63 cooperate to limit the axial travel of the magnetic sleeve 6, so that the magnetic sleeve 6 can only move in the axial space between the boss 64 and the flange 63 when it moves in the axial direction. This is beneficial for the extraction device 7 to drive the magnetic sleeve 6 to move in the axial direction.

[0043] Preferably, a guide slope 65 is provided at the end of the boss 64 away from the flange 63, and the outer diameter of the guide slope 65 gradually decreases in the direction away from the boss 64.

[0044] When the magnetic sleeve 6 is assembled into the liquid cavity 11, there is resistance to the assembly of the magnetic sleeve 6 because the diameter of the outer end face of the boss 64 is larger than the diameter of the opening of the top cover sealing gasket 4. The guide slope 65 can guide the assembly of the magnetic sleeve 6, making it easier for the magnetic sleeve 6 to be assembled into the liquid cavity 11.

[0045] Preferably, there is a radial gap between the outer wall of the boss 64 and the inner wall of the upper shell 2, and the portion between the boss 64 and the flange 63 is defined as a transition section, and there is a radial gap between the inner wall of the flange 21 and the transition section.

[0046] When the magnetic sleeve 6 is in the connected state, the magnetic sleeve 6 is extracted from the liquid cavity 11 by the extraction device 7, so that the flange 21 of the upper outer shell 2 is located between the boss 64 and the flange 63. Since there is a radial gap between the outer wall of the boss 64 and the inner wall of the upper outer shell 2, and a radial gap between the inner wall of the flange 21 and the transition section, the liquid cavity 11 is connected to the outside air through the radial gap and is in an open state. The pressure of the liquid cavity 11 is the same as the outside air pressure, which facilitates liquid transfer and stirring.

[0047] Preferably, the axial distance between the boss 64 and the flange 63 is 4 mm.

[0048] The limiting stroke is matched with the existing extraction device 7 driving the magnetic sleeve 6 to move, which ensures that the extraction device 7 drives the magnetic sleeve 6 to move a sufficient distance, and also ensures that the magnetic sleeve 6 seals with the top cover gasket 4 after entering the liquid chamber 11.

[0049] Preferably, the connecting part includes connecting claws 66 disposed at the tail of the magnetic sleeve 6, each connecting claw 66 being arranged at intervals along the circumference of the magnetic sleeve 6, and an expansion groove for radial expansion and contraction is formed between two adjacent connecting claws 66.

[0050] The connecting part is formed by multiple connecting claws 66. When the extraction device 7 is mounted on or detached from the connecting claws 66, the boss 64 or flange 63 of the magnetic sleeve 6 and the flange 21 of the upper outer shell 2 are blocked. Under the action of the telescopic groove, the connecting claws 66 can be radially contracted and reduced in size, thereby facilitating the magnetic sleeve 6 to detach from or connect with the extraction device 7.

[0051] Preferably, the tail end of the connecting claw 66 is also provided with a stop 67 that is axially blocked by the extraction device 7.

[0052] The baffle 67 can axially block the extraction device 7. When the extraction device 7 drives the magnetic sleeve 6 to detach from the liquid chamber 11 through the connecting claw 66, the baffle 67 provides resistance to the extraction device 7, preventing the extraction device 7 from detaching from the connecting claw 66, and facilitating the extraction device 7 to drive the magnetic sleeve 6 to move axially.

[0053] Preferably, the piston assembly 5 includes a sleeve 51, a piston rod 52 axially movable within the sleeve 51, and a base 53 fixedly connected to the bottom end of the sleeve 51. The base 53 is rotatably mounted on the main body 1. The base 53 has a pipetting hole for communicating with one of the liquid chambers 11 during the rotation stroke and a pipetting channel connecting the sleeve 51 and the pipetting hole.

[0054] When it is necessary to transfer the liquid in one of the liquid chambers 11, rotate the base 53 to connect the pipetting orifice with the liquid chamber 11. After pulling the piston rod 52, a negative pressure is generated in the sleeve 51. Under the pressure, the liquid enters the sleeve 51 through the pipetting orifice and the pipetting channel. Then rotate the base 53 to connect the pipetting orifice with the other liquid chamber 11. Pushing the piston rod 52 will transfer the liquid into the liquid chamber 11.

[0055] The working process of this invention is as follows: In a specific embodiment, the nucleic acid extraction consumable has a total of 12 liquid chambers. The middle chamber is a pull-out liquid chamber. The sleeve 51 and piston rod 52 of the piston assembly 5 are arranged in the middle wall. The pull-out function is used when it is necessary to transfer and mix the liquid evenly. The other chambers are used to arrange the liquids required for nucleic acid extraction, including magnetic beads + proteinase K, lysis buffer, washing buffer, elution buffer, paraffin oil, waste liquid, sample, etc. These liquid chambers 11 are filled with the corresponding liquids during installation. Samples and swabs are sampled and added to the corresponding liquid chambers for later use during testing.

[0056] Both sides of the consumable are sealed with silicone. Each liquid chamber is an independent space and is initially sealed. When it is necessary to extract or transfer liquid from a certain liquid chamber, the base 53 rotates to connect with the corresponding liquid chamber. At the same time, the consumable has a pre-reserved insertion pin for the device before extraction and pushing. The pin directly pierces the silicone. After connecting to atmospheric pressure, the piston rod 52 extracts and pushes the liquid in the sleeve 51 to achieve liquid transfer (if atmospheric pressure is not connected, the liquid chamber of the consumable is in a vacuum state and cannot be extracted).

[0057] When the external extraction device 7 extracts and washes the magnetic beads in the magnetic bead cavity, the magnetic rod 8 is inserted into the inner cavity 62 of the magnetic sleeve 6. The extraction device 7 is connected to the magnetic sleeve 6 and drives the magnetic sleeve 6 to move axially in the magnetic bead cavity to a connected state. The sealing structure of the magnetic sleeve 6 is separated from the top cover sealing gasket 4, and the liquid cavity 11 is connected to the external atmospheric pressure. The magnetic rod 8 of the extraction device 7 can rotate in the magnetic sleeve 6, so that the liquid in the magnetic bead cavity forms a vortex state, thereby cleaning the magnetic beads more effectively.

[0058] After the magnetic attraction is completed, the extraction device 7 pushes the magnetic sleeve 6 into the liquid chamber 11 to a sealed state. The sealing structure and the top cover sealing gasket 4 are sealed together, and a closed space is formed between the sealing structure of the magnetic sleeve 6, the top cover sealing gasket 4 and the liquid chamber 11, completing the subsequent testing work.

[0059] In summary, this invention provides a nucleic acid extraction consumable in which a magnetic sleeve is movably arranged within the magnetic bead cavity of the main body. When an external extraction device elutes the magnetic beads in the magnetic bead cavity, a magnetic rod is inserted into the inner cavity of the magnetic sleeve. The extraction device connects with the magnetic sleeve and drives the magnetic sleeve to move axially within the magnetic bead cavity to a connected state. The sealing structure of the magnetic sleeve separates from the top cover sealing gasket, and the liquid cavity is connected to the external atmospheric pressure. The magnetic rod of the extraction device can rotate within the magnetic sleeve, causing the liquid in the magnetic bead cavity to form a vortex state, thereby more effectively cleaning the magnetic beads and improving the cleaning efficiency. After cleaning, the extraction device drives the magnetic sleeve to move axially within the magnetic bead cavity to a sealed state. The sealing structure and the top cover sealing gasket seal together, forming a sealed space between the sealing structure of the magnetic sleeve, the top cover sealing gasket, and the liquid cavity, completing the subsequent detection work.

[0060] The above description is only a preferred embodiment of the present invention. It should be noted that for those skilled in the art, several improvements and substitutions can be made without departing from the technical principles of the present invention, and these improvements and substitutions should also be considered within the scope of protection of the present invention.

Claims

1. A nucleic acid extraction consumable, characterized in that, The main body includes a plurality of liquid chambers arranged in parallel, the liquid chambers including a magnetic bead chamber for magnetic bead elution, an upper outer shell and a lower outer shell are fitted on the outside of the main body, a top cover sealing gasket is arranged between the upper outer shell and the top surface of the main body, and a piston assembly for transferring liquid in each of the liquid chambers is also arranged on the main body. It also includes a magnetic sleeve, which is movably arranged inside the magnetic bead cavity. The magnetic sleeve is provided with a connecting part for transmission connection with the extraction device to drive the magnetic sleeve to move axially. The magnetic sleeve is provided with an inner cavity for the magnetic rod of the extraction device to be inserted and rotated. The magnetic sleeve is also provided with a sealing structure. During the axial movement stroke, the magnetic sleeve has a sealed state in which the sealing structure and the top cover sealing gasket seal to close the liquid cavity, and a connected state in which the sealing structure and the top cover sealing gasket separate to allow the liquid cavity to communicate with the outside. The sealing structure is a sealing groove arranged on the outside of the magnetic sleeve. The top cover sealing gasket is interference-fitted with the sealing groove. The outer side of the magnetic sleeve is provided with a flange. The flange and the upper outer shell are axially opposed and assembled along the direction in which the magnetic sleeve is inserted into the liquid cavity. The distance between the flange and the sealing groove is equal to the distance between the upper outer shell and the top cover sealing gasket.

2. The nucleic acid extraction consumable according to claim 1, characterized in that, The magnetic sleeve is also provided with a boss, the boss and the flange are arranged at intervals along the axial direction of the magnetic sleeve, the sealing groove is arranged on the boss, and the upper outer shell is provided with an inwardly folded flange. The boss and the flange are both unidirectionally stopped and assembled with the flange.

3. The nucleic acid extraction consumable according to claim 2, characterized in that, The end of the boss away from the flange is provided with a guide slope, and the outer diameter of the guide slope gradually decreases in the direction away from the boss.

4. The nucleic acid extraction consumable according to claim 2, characterized in that, There is a radial gap between the outer wall of the boss and the inner wall of the upper shell, the portion between the boss and the flange is defined as a transition section, and there is a radial gap between the inner wall of the flange and the transition section.

5. The nucleic acid extraction consumable according to claim 2, characterized in that, The axial distance between the boss and the flange is 4 mm.

6. The nucleic acid extraction consumable according to any one of claims 1-5, characterized in that, The connecting part includes connecting claws disposed at the tail of the magnetic sleeve. Each connecting claw is arranged at intervals along the circumference of the magnetic sleeve, and a telescopic groove for radial expansion and contraction is formed between two adjacent connecting claws.

7. The nucleic acid extraction consumable according to claim 6, characterized in that, The tail end of the connecting claw is also provided with a stop that blocks the extraction device axially.

8. The nucleic acid extraction consumable according to any one of claims 1-5, characterized in that, The piston assembly includes a sleeve, a piston rod axially mounted within the sleeve, and a base fixedly connected to the bottom end of the sleeve. The base is rotatably mounted on the main body. The base has a pipetting orifice for communicating with one of the liquid chambers during the rotation stroke and a pipetting channel connecting the sleeve and the pipetting orifice.

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