Chitosanase mutant with altered degree of polymerization of product and use thereof

By performing site-directed mutagenesis on chitosanase BsCsn46A, chitosan oligosaccharide COS4 was generated, which solved the problem of insufficient antibacterial properties of chitosan oligosaccharide in the existing technology and realized the efficient preparation of chitosan oligosaccharide with high antibacterial activity.

CN116656654BActive Publication Date: 2026-07-21CHANGZHOU UNIV
View PDF 0 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
CHANGZHOU UNIV
Filing Date
2023-06-16
Publication Date
2026-07-21

AI Technical Summary

Technical Problem

There is limited research on existing chitosanases for preparing chitosan oligosaccharides with a specified degree of polymerization, making it difficult to generate chitosan oligosaccharide COS4 with high antibacterial activity.

Method used

By performing a site-directed mutation at position 50 of the amino acid sequence of chitosanase BsCsn46A, threonine was mutated to alanine (T50A) or tryptophan (T50W), respectively, to prepare chitosanase mutants COS2 and COS3, which were then used to generate COS4 during the catalytic hydrolysis of chitosan.

Benefits of technology

A large amount of chitosan oligosaccharide COS4 with high antibacterial function was generated, which significantly improved the antibacterial properties of chitosan oligosaccharide.

✦ Generated by Eureka AI based on patent content.

Smart Images

  • Figure FT_1
    Figure FT_1
  • Figure FT_2
    Figure FT_2
Patent Text Reader

Abstract

The application belongs to the technical field of enzyme engineering, and particularly relates to a chitosanase mutant for changing the polymerization degree of a product and application thereof. A 46 family glycoside hydrolase chitosanase (BsCsn46A) derived from Bacillus subtilis has an amino acid sequence as shown in SEQ ID NO:1, and threonine at the 50th position is mutated into a bulky hydrophobic side chain amino acid W or a small volume side chain amino acid A; the mutants are T50A and T50W. Compared with the original enzyme, the chitosanase mutant enzyme hydrolyzes chitosan to generate COS2 and COS3, and also generates chitotetraose (COS4).
Need to check novelty before this filing date? Find Prior Art