Personal care composition

Abstract

Personal care compositions comprising moringa seed oil and moringa extract are disclosed, wherein the amount of moringa seed oil is less than 0.8% by weight of the composition, the amount of moringa extract is less than 0.3% by weight of the composition, the weight ratio of moringa seed oil to moringa extract is in the range of 9:1 to 55:1, and the moringa seed oil is different from the moringa extract.

Description

[0001] Invention Field This invention relates to a personal care composition comprising Moringa seed oil and Moringa extract, wherein the amount of Moringa seed oil is less than 0.8% by weight of the composition, the amount of Moringa extract is less than 0.3% by weight of the composition, and the weight ratio of Moringa seed oil to Moringa extract is in the range of 9:1 to 55:1. In particular, this composition is capable of significantly inhibiting reactive oxygen species. Background of the Invention Human skin provides physicochemical protection against environmental aggressors through its barrier function, mechanical strength, and water impermeability. It is constantly exposed to harmful environments and extreme conditions such as ultraviolet radiation, blue light, and pollution. These harmful environmental exertions can lead to the excessive production of reactive oxygen species (ROS), resulting in oxidative stress. Oxidative stress can affect our skin in many ways, including signs of premature aging such as wrinkles, fine lines, and uneven skin tone.

[0003] Therefore, much effort has been made to develop personal care compositions to prevent the excessive production of reactive oxygen species, but improvements are still needed.

[0004] The inventors have recognized the need for further development of personal care compositions capable of effectively inhibiting the production of reactive oxygen species (ROS). Surprisingly, a combination of small amounts of Moringa extract and Moringa seed oil in specific ratios has been found to exhibit significantly better effects in inhibiting ROS. Invention Overview In a first aspect, the present invention relates to a personal care composition comprising Moringa seed oil and Moringa extract, wherein the amount of Moringa seed oil is less than 0.8% by weight of the composition, the amount of Moringa extract is less than 0.3% by weight of the composition, the weight ratio of Moringa seed oil to Moringa extract is in the range of 9:1 to 55:1, and the Moringa seed oil is different from the Moringa extract.

[0006] In a second aspect, the present invention relates to a method for providing at least one benefit selected from anti-aging, anti-oxidation, or reduction of oxidative stress, comprising the step of applying the personal care composition of the present invention.

[0007] In a third aspect, the present invention relates to the use of the personal care composition of the present invention for at least one benefit selected from anti-aging, anti-oxidation or reduction of oxidative stress.

[0008] All other aspects of the invention will become more apparent from the following detailed description and embodiments. Invention Details Except as expressly stated in the embodiments or elsewhere, all figures in this specification indicating the amount of material or reaction conditions, the physical properties of the material and / or its use may optionally be understood to be modified by the word “about”.

[0010] Unless otherwise stated, all quantities are by weight of the composition.

[0011] It should be noted that when specifying values ​​within any range, any particular upper limit value can be associated with any particular lower limit value.

[0012] To avoid ambiguity, the word "contains" means "includes," but does not necessarily mean "composes of" or "consisting of." In other words, the listed steps or options do not need to be exhaustive.

[0013] The disclosure of the present invention provided herein is intended to cover all embodiments present in claims that are multi-dependent, without regard to the fact that the claims may not be multi-dependent or redundant.

[0014] Where a feature is disclosed with respect to a particular aspect of the invention (e.g., the composition of the invention), such disclosure should also be considered applicable to any other aspect of the invention (e.g., the method of the invention) with the necessary modifications.

[0015] Preferably, the seed oil of the Moringa genus is extracted from Moringa ( Moringa oleifera Moringa oleifera (), Moringa pterygosperma ), Moringa arabic Moringa peregrina ), Pakistan Moringa ( Moringa concanensis ) or elephant leg tree ( Moringa drouhardii Seeds of at least one of the following genus *Moringa*. Seeds of the *Moringa* genus are characterized by the presence of an oil, the content of which can vary from 20% to 80% depending on the species and seed maturity. For *Moringa* (… Moringa oleifera The oil content in Moringa seeds ranges from 21% to 34%. For clarity, Moringa seed oil is typically liquid at 25°C and atmospheric pressure.

[0016] Preferably, Moringa seed oil is derived from Moringa ( Moringa oleifera The oil is extracted from the seeds of Moringa spp. Preferably, the Moringa seed oil is extracted by cold pressing. Preferably, the Moringa seed oil contains C... 20 Fatty acids, preferably behenic acid.

[0017] Preferably, the Moringa seed oil is present in an amount of 0.00001 to 1.5% by weight of the composition, more preferably 0.0001 to 0.5%, even more preferably 0.004 to 0.3%, and even more preferably 0.01 to 0.1%.

[0018] The Moringa extract is an extract selected from at least one of Moringa, Moringa wingedis, Moringa arabic, Moringa santalinus, or Moringa serrata. Preferably, the Moringa extract contains protein. More preferably, the Moringa extract contains 30 to 100%, even more preferably 70 to 100%, of protein by weight of the Moringa extract, and most preferably 100% of protein by weight of the Moringa extract. Preferably, the protein has a molecular weight of 2,000 to 50,000, preferably 5,000 to 20,000, as measured by sodium lauryl sulfate (SDS)-polyacrylamide gel electrophoresis. Preferably, the protein is soluble in water but insoluble in oils and fats.

[0019] Preferably, the Moringa extract is an aqueous extract of the Moringa genus. An aqueous extract is an extract obtained by using water or water mixed with other solvents. The other solvents may be selected from ethanol, acetone, ethyl acetate, glycerol, butylene glycol, or mixtures thereof. Preferably, the aqueous extract is an extract obtained by using water and / or glycerol. Preferably, the Moringa extract is a solid at 25°C and atmospheric pressure.

[0020] Preferably, the Moringa extract is present in an amount of 0.000001 to 0.2% by weight of the composition, more preferably 0.00001 to 0.05%, even more preferably 0.0002 to 0.01%, even more preferably 0.0005 to 0.006%.

[0021] Preferably, the Moringa extract has the INCI name *Moringa oleifera* seed extract. Preferably, the Moringa extract contains PURISOFT. ® (From Laboratories Serobiologues / BASF), its INCI name is glycerol (and) Moringa oleifera seed extract.

[0022] Particularly preferably, the Moringa seed oil and Moringa extract are extracted from the same species. In this case, the protein and oil are preferably extracted from Moringa or Moringa oleifera. For clarity, Moringa seed oil is different from Moringa extract.

[0023] Preferably, the weight ratio of Moringa seed oil to Moringa extract is in the range of 18:1 to 55:1, more preferably in the range of 24:1 to 45:1.

[0024] For clarity, the weight of Moringa seed oil or Moringa extract in this invention generally refers to the weight of all components extracted from Moringa, excluding the weight of the extraction solvent.

[0025] The composition preferably comprises a cleaning surfactant. The composition may contain more than one cleaning surfactant. The cleaning surfactant may be selected from soaps, non-soap anionic, cationic, nonionic, amphoteric surfactants, and mixtures thereof. Many suitable surfactant compounds are available and well described in the literature, for example, in Schwartz, Perry, and Berch's "Surface-Active Agents and Detergents," Volumes I and II. Preferred surfactant compounds that can be used are soaps, non-soap anionic, nonionic surfactants, amphoteric surfactants, or mixtures thereof.

[0026] Suitable non-soap anionic surfactants include linear alkylbenzene sulfonates, primary and secondary alkyl sulfates, especially C8 to C96 sulfates. 15 Primary alkyl sulfates; alkyl ether sulfates; olefin sulfonates; alkyl xylene sulfonates; dialkyl sulfosuccinates; fatty acid ester sulfonates; or mixtures thereof. Sodium salts are generally preferred.

[0027] The most preferred non-soap anionic surfactants are straight-chain alkylbenzene sulfonates, especially those with alkyl chain lengths of C8 to C99. 15 The linear alkylbenzene sulfonate. Preferably, the content of the linear alkylbenzene sulfonate is from 0% to 30% by weight of the total composition, more preferably from 1% to 25% by weight, and most preferably from 2% to 15% by weight.

[0028] The nonionic surfactants that can be used include primary and secondary alcohol ethoxylates, especially C8 to C9 alcohols ethoxylated with an average of 1 to 20 moles of ethylene oxide per mole of alcohol. 20 Fatty alcohols, especially those ethoxylated with an average of 1 to 10 moles of ethylene oxide per mole of alcohol, are particularly prone to oxidation. 10 To C 15 Primary and secondary fatty alcohols. Non-ethoxylated nonionic surfactants include alkyl polysaccharides, glycerol monoethers, and polyhydroxyamides (glucosamides). Preferably, the content of the nonionic surfactant is 0% to 30% by weight of the complete formulation containing the microcapsules of the present invention, more preferably 1% to 25% by weight, and most preferably 2% to 15% by weight.

[0029] Suitable amphoteric surfactants are preferably betaine surfactants. Examples of suitable amphoteric surfactants include, but are not limited to, alkyl betaine, alkylamido betaine, alkyl sulfono betaine, alkyl sulfono betaine, and alkylamido sulfono betaine; preferably, those having 8 to about 18 carbons in the alkyl and acyl groups. Preferably, the amount of the amphoteric surfactant is 0 to 20% by weight of the composition, more preferably 1 to 10% by weight.

[0030] It may also include certain monoalkyl cationic surfactants. Cationic surfactants that can be used include those with the general formula R. 1 R 2 R 3 R 4 N + X - Quaternary ammonium salts, wherein R group is a long or short hydrocarbon chain, typically alkyl, hydroxyalkyl, or ethoxylated alkyl, and X is a counterion (e.g., where R... 1 It is C8 to C 22 Alkyl groups, preferably C8 to C9 10 Or C 12 -C 14 Alkyl, R 2 It is methyl, R 3 and R 4 (These may be the same or different) compounds that are methyl or hydroxyethyl groups); and cationic esters (e.g., choline esters).

[0031] Water-insoluble skin-beneficial agents can also be formulated into compositions as conditioning and moisturizing agents. Examples include silicone oils; hydrocarbons such as liquid paraffin, petrolatum, microcrystalline wax, and mineral oils; and vegetable triglycerides such as sunflower seed oil and cottonseed oil.

[0032] Some compositions may include thickeners. These may be selected from, but are not limited to, cellulose-based, natural gums and acrylic polymers. Cellulose-based thickeners include sodium carboxymethyl cellulose, hydroxyethyl cellulose, hydroxypropyl methyl cellulose, and combinations thereof. Suitable gums include xanthan gum, pectin, ebony gum, agar, alginate, and combinations thereof. Acrylic thickeners include homopolymers and copolymers of acrylic acid and methacrylic acid, including carbomers such as Carbopol 1382, Carbopol 982, Ultrez, Aqua SF-1, and Aqua SF-2, available from Lubrizol Corporation. The amount of thickener may range from 0.01% to 3% by weight of the active polymer in the composition (excluding solvent or water).

[0033] Preservatives can be ideally incorporated into the compositions of the present invention to prevent the growth of potentially harmful microorganisms. Conventional preservatives suitable for the compositions of the present invention are alkyl esters of hydrobenzoic acid. Other preservatives recently introduced include hydantoin derivatives, propionates, and various quaternary ammonium compounds. Particularly preferred preservatives are phenoxyethanol, methylparaben, propylparaben, imidazolidinyl urea, sodium dehydroacetate, and benzyl alcohol. The selection of preservatives should take into account the intended use of the composition and possible incompatibilities between the preservative and other components. Preservatives are preferably used in amounts from 0.01% to 2% by weight of the composition.

[0034] A variety of other optional materials can be formulated into the composition. These may include: antimicrobial agents such as 2-hydroxy-4,2',4'-trichlorodiphenyl ether (triclosan), 2,6-dimethyl-4-hydroxychlorobenzene, and 3,4,4'-trichlorocarbonylaniline; exfoliating and dermal exfoliating particles such as polyethylene and silica or alumina; cooling agents such as menthol; skin soothing agents such as aloe vera; and colorants.

[0035] In addition, the compositions of the present invention may also contain 0.5 to 10% by weight of a chelating agent, such as tetrasodium ethylenediaminetetraacetic acid (EDTA), EHDP or a mixture thereof; an opacifier and a pearlescent agent, such as ethylene glycol distearate, titanium dioxide or Lytron 621 (styrene / acrylate copolymer); all of which can be used to improve the appearance or performance of the product.

[0036] The composition may contain 10 to 90% water by weight of the composition, more preferably 20 to 85% by weight of the composition, even more preferably 25 to 78% by weight of the composition, and most preferably 32 to 65% by weight of the composition.

[0037] Preferably, when in about 20 s -1 When measured at a relatively high shear rate at 20°C, the composition has a viscosity of at least 10 mPa·s, more preferably in the range of 30 to 10000 mPa·s, even more preferably 50 to 5000 mPa·s, and most preferably in the range of 100 to 2000 mPa·s. Preferably, the composition is in fluid form.

[0038] Preferably, the personal care composition is a skin care composition. As used herein, the term "skin" includes the skin of the face, neck, chest, abdomen, back, arms, armpits, hands, and legs. Preferably, "skin" refers to the skin including the face and armpits; more preferably, skin refers to the facial skin excluding the lips and eyelids.

[0039] Preferably, the method is non-therapeutic. Preferably, the use is non-therapeutic. The term "non-therapeutic" means used for cosmetic purposes, rather than for curative or therapeutic purposes.

[0040] The present invention also provides a personal care composition comprising Moringa seed oil and Moringa extract, wherein the amount of Moringa seed oil is less than 0.8% by weight of the composition, the amount of Moringa extract is less than 0.3% by weight of the composition, and the weight ratio of Moringa seed oil to Moringa extract is in the range of 9 to 55, which is intended to provide at least one benefit selected from anti-aging, antioxidant, or reduction of oxidative stress (particularly due to light exposure).

[0041] The present invention also provides the use of a combination of less than 0.8% by weight of Moringa seed oil and less than 0.3% by weight of Moringa extract in the preparation of a medicament for providing at least one benefit selected from anti-aging, antioxidant or oxidative stress reduction, wherein the weight ratio of Moringa seed oil to Moringa extract is in the range of 9 to 55.

[0042] The following embodiments are provided to facilitate understanding of the invention. These embodiments are not intended to limit the scope of the claims.

[0043] Example Material

[0044] Purisoft ® LS9726 was purchased from BASF Beauty Solutions France SAS.

[0045] Moringa oil (LA030075-refined) was purchased from Naturex SA in France.

[0046] Example 1 Normal human epidermal keratinocytes (NHEKs) (Biocell, Xi'an, China) were cultured in keratinocyte culture medium and seeded in 6-well plates. On the second day, the culture medium was replaced with medium containing the test samples.

[0047] Moringa oil was dissolved in DMSO (dimethyl sulfoxide) at a concentration of 100 mM to form a stock solution. Samples were prepared accordingly in Table 1 by diluting the materials (excluding moringa oil) and / or the stock solution with PBS (phosphate-buffered saline) to the desired levels shown in Table 1 prior to use in further experiments.

[0048] After incubation for another 24 hours in each sample, the culture medium was replaced with PBS. The cells were then irradiated with blue light using a blue LED (PL-L 18 W / 52 / 4P, Philips, Poland), which emitted light almost entirely within a 400–500 nm bandwidth, with a peak at 450 nm. Irradiation was measured using a blue light safety detector (PMA2121) (SolarLight, PA, USA), with a total energy of 10 J / cm². 2Thirty minutes after blue light irradiation, ROS (reactive oxygen species) production was assessed using DCFH-DA (2',7'-dichlorodihydrofluorescein diacetate) dye and detected by flow cytometry (CytoFLEX, Beckman Coulter, USA). Data were acquired and analyzed using the CytExpert program (Beckman Coulter, USA).

[0049] The results are summarized in Table 1.

[0050] Table 1

[0051] Content refers to the content of raw materials, expressed as a percentage by weight.

[0052] The ratio refers to the ratio of active substances.

[0053] a: No significant improvement over at least one sample containing a single active substance (p<0.05, 3 vs 1 or 2; 9 vs 1 or 8).

[0054] b: Significantly better than the two samples containing a single active substance (p<0.05, 5 vs. 1 or 4; 7 vs. 1 or 6).

[0055] The data in Table 1 clearly show that when a small amount of Moringa seed oil and Moringa extract are combined in the specific proportions of this invention, the inhibition of reactive oxygen species is significantly better.

Claims

1. A personal care composition comprising Moringa seed oil and Moringa extract, wherein: (a) The amount of Moringa seed oil is 0.0001 to 0.5% of the weight of the composition; (b) The amount of the Moringa extract is 0.00001 to 0.05% of the weight of the composition; (c) The weight ratio of the Moringa seed oil to the Moringa extract is in the range of 9:1 to 45:1, wherein the weight of the Moringa seed oil or Moringa extract refers to the weight of all components extracted from Moringa, excluding the weight of the extraction solvent. (d) The Moringa seed oil is liquid at 25°C and atmospheric pressure, and the Moringa extract is solid at 25°C and atmospheric pressure; (e) The Moringa extract is an aqueous extract of the Moringa genus; and (f) The Moringa seed oil is different from the Moringa extract.

2. The composition according to claim 1, wherein the Moringa seed oil is extracted from the seeds of at least one of Moringa, Moringa wingedis, Moringa arabic, Moringa santalinus, and Moringa serrata.

3. The composition according to claim 1 or 2, wherein the Moringa seed oil is present in an amount of 0.004 to 0.3% by weight of the composition.

4. The composition according to claim 1 or 2, wherein the Moringa extract comprises a protein.

5. The composition according to claim 1 or 2, wherein the Moringa extract comprises 30% to 100% protein by weight of the Moringa extract.

6. The composition according to claim 1 or 2, wherein the Moringa extract has the INCI name *Moringa oleifera* seed extract.

7. The composition according to claim 1 or 2, wherein the Moringa extract is present in an amount of 0.0002 to 0.01% by weight of the composition.

8. The composition according to claim 1 or 2, wherein the weight ratio of the Moringa seed oil to the Moringa extract is in the range of 18:1 to 45:

1.

9. The composition according to claim 1 or 2, wherein the weight ratio of the Moringa seed oil to the Moringa extract is in the range of 24:1 to 45:

1.

10. The composition according to claim 1 or 2, wherein the composition comprises 25% to 78% by weight of water.

11. A non-therapeutic method for providing at least one benefit selected from anti-aging, antioxidant and oxidative stress reduction, comprising the step of applying a personal care composition according to any one of claims 1 to 10.

12. The personal care composition according to any one of claims 1 to 10 for non-therapeutic use of at least one of the benefits selected from anti-aging, antioxidant and oxidative stress reduction.

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