Optical system
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- GEOPTICS SEQUENCING EQUIP CO LTD
- Filing Date
- 2021-02-05
- Publication Date
- 2026-07-24
AI Technical Summary
Existing technologies make it difficult to achieve high numerical aperture, large field of view and full field of view diffraction limit microscope objectives in high-throughput gene sequencers, and existing designs are complex and difficult to mass-produce.
An optical system consisting of multiple spherical lenses, including lenses one through thirteen, is used to provide field curvature and chromatic aberration correction through the combination of cemented lens groups. This achieves a flat field of view with diffraction limit, a numerical aperture of 0.8, and a field diameter of 1.52 mm.
It achieves high information capacity in a small volume, simplifies the processing and assembly process, supports mass production, and is suitable for high-throughput gene sequencing of various fluorescent materials.
Smart Images
Figure CN116710829B_ABST
Abstract
Description
Technical Field
[0001] This application relates to the field of optical imaging technology, and in particular to an optical system. Background Technology
[0002] One of the core components of high-throughput gene sequencers is fluorescence microscopy. This involves exciting gene samples with illumination to produce fluorescence, then separating the relatively weak fluorescence signals for imaging. This transforms invisible microscopic information into visual images and data, enabling the identification of gene bases. The core component of fluorescence microscopy is the fluorescence microscope objective. The most crucial factor determining the throughput of a gene sequencer is the number of gene samples that the fluorescence microscope objective can resolve in a single image. This can be measured by the information capacity of the fluorescence microscope objective, which can be expressed using Lagrange invariants, i.e., the product of the numerical aperture and the field of view diameter. Therefore, high numerical aperture and a large field of view have always been the goals pursued in the design of microscope objectives.
[0003] Because fluorescence is affected by optical aberrations caused by the design and manufacturing of microscope objectives, the central field of view of fluorescence microscope objectives typically exhibits very small aberrations, achieving an information capacity that is Lach's invariant. However, the peripheral field of view often suffers from reduced resolution due to aberrations such as greater field curvature. In other words, the peripheral field of view does not utilize the upper limit of its numerical aperture resolution, and its information capacity is less than that of the Lach's invariant. Since gene sequencing imaging requires fluorescence microscope objectives to provide uniform imaging across the entire field of view, meaning that the resolution of the peripheral field of view must be the same as that of the central field of view, and both must achieve the Lach's invariant information capacity to maximize the utilization of numerical aperture and improve sequencing throughput, high-throughput sequencing necessitates the development of high-information-capacity microscope objectives—that is, microscope objectives with high numerical aperture, large field of view, and flat field of view that achieve diffraction-limited performance across the entire field of view.
[0004] In the process of developing this application, the inventors discovered the following problems in the prior art: High-information-capacity microscope objectives require simultaneous achievement of high numerical aperture, large field of view, and full-field diffraction limit. However, these three aspects are contradictory in design. Low-magnification microscope objectives have a large field of view but a small numerical aperture, while high-magnification microscope objectives have a large numerical aperture but a small field of view. High numerical aperture and large field of view will lead to significant edge field-of-view aberrations. Existing microscope objectives that can simultaneously achieve high numerical aperture, large field of view, and full-field diffraction limit employ catadioptric optical structures, resulting in large size and complex manufacturing and assembly processes, making mass production difficult. Summary of the Invention
[0005] In view of the above, it is necessary to propose an optical system to solve the above problems.
[0006] Embodiments of this application propose an optical system comprising a first lens, a second lens, a third lens, a fourth lens, a fifth lens, a sixth lens, a seventh lens, an eighth lens, a ninth lens, a tenth lens, an eleventh lens, a twelfth lens, and a thirteenth lens arranged sequentially along the optical axis from the image side to the object side. The first lens and the second lens are cemented together to form a first cemented lens group with positive optical power. The third lens, the fourth lens, and the fifth lens are cemented together to form a second cemented lens group with negative optical power. The sixth lens and the seventh lens are cemented together to form a third cemented lens group with positive optical power. The eighth lens and the ninth lens are cemented together to form a fourth cemented lens group with positive optical power. The tenth lens and the eleventh lens are cemented together to form a fifth cemented lens group with positive optical power.
[0007] In some embodiments, the first lens to the thirteenth lens are all spherical lenses.
[0008] In some embodiments, the first lens, the fourth lens, the sixth lens, the eighth lens, the tenth lens, the twelfth lens, and the thirteenth lens all have positive optical power.
[0009] In some embodiments, the image-side surface and object-side surface of the first lens, the eighth lens, and the tenth lens are both convex surfaces; the image-side surface of the fourth lens and the sixth lens is either convex or flat, and the object-side surface is convex; the image-side surface of the twelfth lens is convex, and the object-side surface is either convex or flat.
[0010] In some embodiments, the image-side surface of the thirteenth lens is convex, and the object-side surface of the thirteenth lens is concave near the optical axis.
[0011] In some embodiments, the second lens, the third lens, the fifth lens, the seventh lens, the ninth lens, and the eleventh lens all have negative optical power.
[0012] In some embodiments, the object-side surface and the image-side surface of the second lens and the eleventh lens are both concave; the image-side surface of the third lens is concave, and the object-side surface is either flat or concave; the image-side surface of the fifth lens, the seventh lens, and the ninth lens are all concave, and the object-side surface is always convex.
[0013] In some embodiments, the Abbe number of the fourth lens, the sixth lens, the eighth lens, and the tenth lens is greater than 80.
[0014] In some embodiments, the Abbe number of the twelfth lens is greater than 70; the refractive index of the thirteenth lens is greater than 1.75.
[0015] In some embodiments, an aperture stop is also included, which is disposed between the ninth lens and the tenth lens to limit the amount of light transmitted.
[0016] The optical structure composed of the first and second cemented lens groups in the aforementioned optical system provides strong field curvature correction and transverse chromatic aberration correction, ensuring that the diffraction limit is reached across the entire flat field of view. The optical structure composed of the third, fourth, and fifth cemented lens groups provides strong axial chromatic aberration and second-order spectral correction, ensuring sufficient imaging spectral width. The numerical aperture of the optical system of this application can reach 0.8, the field diameter can reach 1.52 mm, and it can achieve the diffraction limit across the entire flat field of view. It achieves high information capacity in a small volume, has a simple processing and assembly process, and can be mass-produced. Attached Figure Description
[0017] Figure 1 This is a schematic diagram of the structure and optical path of the optical system according to an embodiment of this application.
[0018] Figure 2 This is a schematic diagram of the wave aberration and field of view of spectral band 1 of the optical system according to an embodiment of this application.
[0019] Figure 3 This is a schematic diagram of the wave aberration and field of view of the optical system in spectral band 2 of this application embodiment.
[0020] Figure 4 This is a schematic diagram of the wave aberration and field of view of the optical system in spectral band 3 according to an embodiment of this application.
[0021] Figure 5 This is a schematic diagram of the wave aberration and field of view of spectral band 4 of the optical system according to an embodiment of this application.
[0022] Explanation of main component symbols
[0023] Optical System 10
[0024] First lens L1
[0025] Second lens L2
[0026] Third lens L3
[0027] Fourth lens L4
[0028] Fifth lens L5
[0029] Sixth lens L6
[0030] Seventh lens L7
[0031] Eighth lens L8
[0032] Ninth Lens L9
[0033] Tenth lens L10
[0034] Eleventh Lens L11
[0035] Twelfth Lens L12
[0036] The thirteenth lens L13
[0037] First cemented lens group G1
[0038] Second cemented lens group G2
[0039] Third cemented lens group G3
[0040] Fourth cemented lens group G4
[0041] Fifth cemented lens group G5
[0042] STO aperture
[0043] Like the side views S1, S2, S4, S5, S6, S8, S9, S11,
[0044] S12, S15, S16, S18, S20
[0045] Side surfaces S3, S6, S7, S10, S13, S17, S19, S21 Detailed Implementation
[0046] To make the objectives, technical solutions, and advantages of this application clearer, the following detailed description is provided in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative and not intended to limit the scope of this application. All other embodiments obtained by those skilled in the art based on the embodiments in this application without inventive effort are within the scope of protection of this application.
[0047] In the description of this application, it should be understood that the terms "center," "longitudinal," "lateral," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," and "counterclockwise," etc., indicating orientation or positional relationships based on the orientation or positional relationships shown in the accompanying drawings, are only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this application. Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Thus, features defined with "first" and "second" may explicitly or implicitly include one or more features. In the description of this application, "multiple" means two or more, unless otherwise explicitly specified.
[0048] In the description of this application, it should be noted that, unless otherwise expressly specified and limited, the terms "installation," "connection," and "linking" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection, an electrical connection, or a connection that allows communication between them; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication between two components or the interaction between two components. Those skilled in the art can understand the specific meaning of the above terms in this application according to the specific circumstances.
[0049] In this application, unless otherwise expressly specified and limited, "above" or "below" the second feature can include direct contact between the first and second features, or contact between the first and second features through another feature between them. Furthermore, "above," "over," and "on top" of the second feature includes the first feature being directly above or diagonally above the second feature, or simply indicates that the first feature is at a higher horizontal level than the second feature. "Below," "below," and "under" the second feature includes the first feature being directly above or diagonally above the second feature, or simply indicates that the first feature is at a lower horizontal level than the second feature.
[0050] The following disclosure provides many different embodiments or examples for implementing different structures of this application. To simplify the disclosure, specific examples of components and arrangements are described below. Of course, these are merely examples and are not intended to limit the scope of this application. Furthermore, reference numerals and / or letters may be repeated in different examples; such repetition is for simplification and clarity and does not in itself indicate a relationship between the various embodiments and / or arrangements discussed. In addition, various specific examples of processes and materials are provided in this application, but those skilled in the art will recognize the application of other processes and / or the use of other materials.
[0051] Please see Figure 1 This application provides an optical system 10 for use in a microscope objective. From the image side to the object side along the optical axis, it includes, in sequence, a first lens L1, a second lens L2, a third lens L3, a fourth lens L4, a fifth lens L5, a sixth lens L6, a seventh lens L7, an eighth lens L8, a ninth lens L9, an aperture stop STO, a tenth lens L10, an eleventh lens L11, a twelfth lens L12, and a thirteenth lens L13. The first lens L1 to the thirteenth lens L13 are all spherical lenses and together constitute a microscope objective optical system 10 with high information capacity.
[0052] Specifically, the first lens L1 has positive optical power, and both the image-side surface S1 and the object-side surface of the first lens L1 are convex.
[0053] Understandably, in other embodiments, the image-side surface S1 of the first lens L1 is planar, while the object-side surface is convex.
[0054] The second lens L2 has negative optical power, and both its image-side surface S2 and object-side surface S3 are concave. The first lens L1 and the second lens L2 are cemented together to form a first cemented lens group G1 with positive optical power.
[0055] It should be noted that the object-side surface of the first lens L1 and the image-side surface S2 of the second lens L2 share a common surface. In this embodiment, only one of the surfaces is labeled, namely the image-side surface S2 of the second lens L2. This situation will not be repeated in the following text.
[0056] The third lens L3 has negative optical power, and both the image-side surface S4 and the object-side surface of the third lens L3 are concave.
[0057] Understandably, in other embodiments, the image-side surface S4 of the third lens L3 is concave, and the object-side surface is planar.
[0058] The fourth lens L4 has positive optical power, and both its image-side surface S5 and object-side surface are convex. In this embodiment, the fourth lens L4 is made of an ultra-low dispersion material, meaning its Abbe number is greater than 80, which is beneficial for chromatic aberration correction.
[0059] Understandably, in other embodiments, the image-side surface S5 of the fourth lens L4 is planar, and the object-side surface is convex.
[0060] The fifth lens L5 is a meniscus lens with negative optical power. The image-side surface S6 of the fifth lens L5 is concave, and the object-side surface S7 of the fifth lens L5 is convex. The third lens L3, the fourth lens L4, and the fifth lens L5 are cemented together to form a second cemented lens group G2 with negative optical power.
[0061] In this embodiment, the optical structure composed of the first cemented lens group G1 and the second cemented lens group G2 can provide strong field curvature correction and transverse chromatic aberration correction, ensuring that the flat field of view reaches the diffraction limit.
[0062] The sixth lens L6 has positive optical power. The image side S8 of the sixth lens L6 is convex, and the object side of the sixth lens L6 is also convex. This is beneficial for the correction of chromatic aberration.
[0063] Understandably, in other embodiments, the image-side surface S8 of the sixth lens L6 is planar, and the object-side surface is convex.
[0064] The seventh lens L7 is a meniscus lens with negative optical power. The image-side surface S9 of the seventh lens L7 is concave, and the object-side surface S10 of the seventh lens L7 is convex. The sixth lens L6 and the seventh lens L7 are cemented together to form the third cemented lens group G3 with positive optical power.
[0065] The eighth lens L8 has positive optical power, and both the image-side surface S11 and the object-side surface of the eighth lens L8 are convex.
[0066] The ninth lens L9 is a meniscus lens with negative optical power. The image-side surface S12 of the ninth lens L9 is concave, and the object-side surface S13 of the ninth lens L9 is convex. The eighth lens L8 and the ninth lens L9 are cemented together to form the fourth cemented lens group G4 with positive optical power.
[0067] The tenth lens L10 has positive optical power, and both the image-side surface S15 and the object-side surface of the tenth lens L10 are convex.
[0068] The eleventh lens L11 has negative optical power, and both its image-side surface S16 and object-side surface S17 are concave. The tenth lens L10 and the eleventh lens L11 are cemented together to form the fifth cemented lens group G5, which has positive optical power.
[0069] In this embodiment, the sixth lens L6, the eighth lens L8, and the tenth lens L10 all use ultra-low dispersion materials, meaning that the Abbe numbers of the sixth lens L6, the eighth lens L8, and the tenth lens L10 are all greater than 80. This is beneficial for chromatic aberration correction. Thus, the optical structure composed of the third cemented lens group G3, the fourth cemented lens group G4, and the fifth cemented lens group G5 provides strong axial chromatic aberration and second-order spectral correction, ensuring sufficient imaging spectral width.
[0070] The twelfth lens L12 has positive optical power, and both the object side S19 and the image side S18 of the twelfth lens L12 are convex surfaces.
[0071] Understandably, in other embodiments, the image-side surface S18 of the twelfth lens L12 is planar, and the object-side surface S19 is convex.
[0072] The thirteenth lens L13 is a thick meniscus lens with positive optical power. The image-side surface S20 of the thirteenth lens L13 is convex, and the object-side surface S21 of the thirteenth lens L13 is concave. The thick meniscus lens is characterized by a certain distance between the image-side surface S20 and the object-side surface S21 of the thirteenth lens L13.
[0073] In this embodiment, the twelfth lens L12 is made of a low-dispersion material, meaning its Abbe number is greater than 70, which is beneficial for chromatic aberration correction. The thirteenth lens L13 is made of a high-refractive-index material, meaning its refractive index is greater than 1.75. This is beneficial for spherical aberration correction. This optical structure, consisting of the low-dispersion twelfth lens L12 and the high-refractive-index thirteenth lens L13, provides a large numerical aperture and low chromatic aberration.
[0074] The aperture stop STO can be positioned anywhere between the sixth lens L6 and the eleventh lens L11. In this embodiment, the aperture stop STO is positioned between the ninth lens L9 and the tenth lens L10. Thus, an optical system 10 with a smaller numerical aperture can be achieved by reducing the aperture stop.
[0075] In this embodiment, as Figure 1 As shown, the object space medium of the optical system 10 is air, and the optical working distance is the distance from the right vertex of the thirteenth lens L13 to the object surface or the cover glass. The optical working distance can be selectively designed to be 1 mm to 2 mm. The object space of the optical system 10 can be selectively fitted with a cover glass and a liquid layer. The cover glass can be a flat plate of any material that transmits visible light, and its thickness can be selectively designed to be 0.1 mm to 0.3 mm. The liquid layer can be any liquid that transmits visible light, and its thickness can be selectively designed to be 0.01 mm to 0.2 mm.
[0076] This embodiment can be applied to gene sequencing. The four fluorescence bands are 550nm~580nm, 605nm~635nm, 660nm~690nm and 715nm~745nm, respectively. The four fluorescence bands are parfocal and super-achromatic.
[0077] As shown in Table 1, the numerical aperture of the microscope objective optical system in this embodiment is 0.8, the field of view diameter is 1.52 mm, the focal length is 10 mm, and the optical working distance is 1.24 mm.
[0078] Table 1
[0079] Operating band 550nm~580nm, 605nm~635nm, 660nm~690nm and 715nm~745nm Numerical aperture 0.8 Object field of view Field of view diameter 1.52mm focal length 10mm working distance 1.24mm
[0080] Please refer to Table 2. The specific parameters of the optical system 10 in this embodiment are as follows. The units for radius, spacing, thickness and half-aperture are all millimeters (mm).
[0081] Table 2
[0082]
[0083] Figure 2 This is a schematic diagram of wave aberration and field of view for spectral band 1. The working wavelength range of spectral band 1 is 550nm to 580nm. From the diagram, it can be seen that the wave aberration reaches the diffraction limit across the entire field of view.
[0084] Figure 3 This is a schematic diagram of wavefront aberration and field of view for spectral band 2. The operating wavelength range for spectral band 2 is 605 nm to 635 nm. The diagram shows that the wavefront aberration reaches the diffraction limit across the entire field of view.
[0085] Figure 4 This is a schematic diagram of wavefront aberration and field of view for spectral band 3. The operating wavelength range for spectral band 3 is 660 nm to 690 nm. The diagram shows that wavefront aberration reaches the diffraction limit across the entire field of view.
[0086] Figure 5 This is a schematic diagram of wave aberration and field of view for spectral band 4. The operating wavelength of spectral band 4 is 715nm to 745nm. From the diagram, it can be seen that the wave aberration reaches the diffraction limit across the entire field of view.
[0087] The optical structure composed of the first cemented lens group G1 and the second cemented lens group G2 in the optical system 10 described above provides strong field curvature correction and transverse chromatic aberration correction, ensuring that the diffraction limit is reached in the flat field of view. The optical structure composed of the third cemented lens group G3, the fourth cemented lens group G4 and the fifth cemented lens group G5 provides strong axial chromatic aberration and second-order spectral correction, ensuring sufficient imaging spectral width. The optical system 10 of this embodiment has a wide operating band in the visible band, is suitable for a variety of fluorescent materials, and has a numerical aperture of 0.8, a field diameter of 1.52 mm, and an object-side field diameter of 1.52 mm. It can also achieve the diffraction limit in the flat field of view, achieving high information capacity in a small volume. The first lens L1 to the thirteenth lens L13 are all spherical lenses, which are easy to process and have simple assembly processes, and can be mass-produced.
[0088] It will be apparent to those skilled in the art that this application is not limited to the details of the exemplary embodiments described above, and that this application can be implemented in other specific forms without departing from the spirit or essential characteristics of this application. Therefore, the embodiments should be regarded as exemplary and non-limiting in all respects, and the scope of this application is defined by the appended claims rather than the foregoing description. Thus, all variations falling within the meaning and scope of equivalents of the claims are intended to be embraced within this application.
[0089] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of this application and are not intended to limit it. Although this application has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of this application without departing from the spirit and scope of the technical solutions of this application.
Claims
1. An optical system comprising a first lens, a second lens, a third lens, a fourth lens, a fifth lens, a sixth lens, a seventh lens, an eighth lens, a ninth lens, a tenth lens, an eleventh lens, a twelfth lens, and a thirteenth lens arranged sequentially along the optical axis from the image side to the object side, characterized in that, The optical system has a total of thirteen lenses; The first lens and the second lens are cemented together to form a first cemented lens group with positive optical power. The third lens, the fourth lens, and the fifth lens are cemented together to form a second cemented lens group with negative optical power. The sixth lens and the seventh lens are cemented together to form a third cemented lens group with positive optical power. The eighth lens and the ninth lens are cemented together to form a fourth cemented lens group with positive optical power. The tenth lens and the eleventh lens are cemented together to form a fifth cemented lens group with positive optical power; There is an air gap between the twelfth lens, the fifth cemented lens group, and the thirteenth lens. The first lens, the fourth lens, the sixth lens, the eighth lens, the tenth lens, the twelfth lens, and the thirteenth lens all have positive optical power, while the second lens, the third lens, the fifth lens, the seventh lens, the ninth lens, and the eleventh lens all have negative optical power.
2. The optical system as claimed in claim 1, characterized in that, The first lens through the thirteenth lens are all spherical lenses.
3. The optical system as described in claim 1, characterized in that, The image-side and object-side surfaces of the first lens, the eighth lens, and the tenth lens are all convex; the image-side surface of the fourth lens and the sixth lens is convex or flat, and the object-side surface is convex; the image-side surface of the twelfth lens is convex, and the object-side surface is convex or flat.
4. The optical system as claimed in claim 1, characterized in that, The image-side surface of the thirteenth lens is convex, and the object-side surface of the thirteenth lens is concave near the optical axis.
5. The optical system as claimed in claim 1, characterized in that, The object-side and image-side surfaces of the second lens and the eleventh lens are both concave; the image-side surface of the third lens is concave, and the object-side surface is either flat or concave; the image-side surfaces of the fifth lens, the seventh lens, and the ninth lens are all concave, and the object-side surfaces are all convex.
6. The optical system as claimed in claim 1, characterized in that, The Abbe numbers of the fourth lens, the sixth lens, the eighth lens, and the tenth lens are all greater than 80.
7. The optical system as claimed in claim 1, characterized in that, The twelfth lens has an Abbe number greater than 70; the thirteenth lens has a refractive index greater than 1.
75.
8. The optical system as claimed in claim 1, characterized in that, It also includes an aperture stop, which is located between the ninth lens and the tenth lens to limit the amount of light passing through.