An air microbe detection system

Abstract

The application discloses an air microorganism detection system, and relates to the technical field of microorganism detection, which comprises a sampling tube, a nucleic acid transfer mechanism, a chip, a driving device and a control system. A sampling cavity is arranged in the sampling tube, and magnetic beads and a sampling liquid are arranged in the sampling cavity in advance, so that the sampling tube is used for sampling microorganisms in air. An elution cavity is arranged in the nucleic acid transfer mechanism. An eluent is arranged in the elution cavity in advance. The sampling tube, the nucleic acid transfer mechanism and the chip are sequentially arranged. The control system controls the driving device to work. The driving device can input the magnetic beads, on which nucleic acids are adsorbed, into the elution cavity, drive the magnetic beads to elute nucleic acids in the eluent, and then input the eluent into the chip for detection. The air microorganism detection system can reduce the degree of manual participation, improve the degree of automation, and contribute to the realization of fully automatic detection.

Description

Technical Field

[0001] This invention relates to the field of microbial detection technology, and in particular to an airborne microbial detection system. Background Technology

[0002] Microorganisms from humans, animals, plants, and soil can be dispersed into the air through droplets or dust, resulting in a certain variety and quantity of microorganisms in the air. While pathogenic microorganisms are generally not present in the air, aerosols containing pathogenic microorganisms are often suspended in the air near hospitals, veterinary clinics, and livestock sheds. Healthy individuals or animals can become infected through inhalation. Air contaminated with pathogenic microorganisms can often become a source or medium of pollution, causing outbreaks of infectious diseases. Therefore, airborne microbial testing is of great significance for the prevention and control of infectious diseases, as well as for the hygienic monitoring and protection of the environment.

[0003] Existing methods for detecting microorganisms in the air mainly rely on manual labor. For example, the patent application with application number 202211081041.9, although it adopts an integrated design to realize multiple processes such as sampling, elution and detection, still requires manual operation by staff during use, which requires a high degree of human intervention and has a low degree of automation. Summary of the Invention

[0004] The purpose of this invention is to provide an airborne microbial detection system to solve the problems existing in the prior art, reduce the degree of human intervention, improve the degree of automation, and contribute to the realization of fully automated detection.

[0005] To achieve the above objectives, the present invention provides the following solution:

[0006] This invention provides an airborne microbial detection system, comprising a sampling tube, a nucleic acid transfer mechanism, a chip, a driving device, and a control system. The sampling tube contains a sampling chamber pre-filled with magnetic beads and a sampling solution, and the sampling tube is used to sample microorganisms in the air. The nucleic acid transfer mechanism contains an elution chamber pre-filled with an elution solution. The sampling chamber is connected to the elution chamber, and the elution chamber is connected to the detection chamber of the chip.

[0007] The control system controls the operation of the driving device, which can input magnetic beads with nucleic acid adsorbed in the sampling chamber into the elution chamber, and can also drive the magnetic beads to perform nucleic acid elution in the elution solution. After the nucleic acid elution is completed, the elution solution can also be input into the chip for detection.

[0008] Preferably, it also includes a magnetic rod, a turntable drive device, and a turntable; the sampling tube, the nucleic acid transfer mechanism, and the chip are arranged in sequence;

[0009] The sampling chamber and the elution chamber are connected by a first channel between them; a second channel is provided on the side of the elution chamber away from the first channel, and the second channel is connected to the sample inlet of the detection chamber. A first transfer valve and a second transfer valve are respectively movably arranged at the first channel and the second channel. The first transfer valve can close the first channel and rotating the first transfer valve can transfer the magnetic beads in the sampling chamber to the elution chamber. The second transfer valve can close the second channel and rotating the second transfer valve can transfer the eluent to the sample inlet.

[0010] The control system controls the driving device to drive the first transfer valve and the second transfer valve to rotate. Both the first transfer valve and the second transfer valve are provided with magnetic rod cavities. The control system can control the driving device to drive the magnetic rod to be inserted into any one of the magnetic rod cavities.

[0011] The turntable is vertically mounted on the frame and can rotate on its own. The turntable driving device can drive the turntable to rotate. The sampling tube, the chip, the nucleic acid transfer mechanism, the driving device and the magnetic rod are all located on one side of the turntable.

[0012] In the sampling state, the integrated unit consisting of the sampling tube, the nucleic acid transfer mechanism, and the chip is in a vertical position.

[0013] During the transfer and elution of magnetic beads, the turntable drives the integrated body to rotate and makes the integrated body horizontal. The driving device drives the magnetic rod to move the magnetic beads to realize the transfer and elution of magnetic beads.

[0014] Preferably, the driving device includes a first motor, a second motor, a connecting rod, and a transmission shaft. The transmission shaft is rotatably mounted on the turntable. The first motor is connected to one end of the transmission shaft. The second motor is fixed on the transmission shaft, and the motor shaft of the second motor is perpendicular to the transmission shaft. One end of the connecting rod is fixed on the motor shaft of the second motor, and the other end extends toward one side of the elution chamber and is fixed with the magnetic rod. The second motor can drive the magnetic rod to align with any one of the magnetic rod cavities, and the first motor can drive the magnetic rod to insert into the magnetic rod cavity.

[0015] Preferably, the device further includes a heating mechanism and an image acquisition mechanism. The chip is made of a transparent material. The heating mechanism includes a heating plate and a heating plate driving device. The heating plate is located at the bottom of the chip and is rotatably mounted. The heating plate driving device can drive the heating plate to rotate. The image acquisition mechanism is located at the bottom of the chip. When the heating plate rotates to be close to the chip, it can heat the chip. When the heating plate rotates away from the chip, the image acquisition mechanism can acquire images of the detection results in the chip.

[0016] Preferably, the sampling tube, the nucleic acid transfer mechanism, and the chip are all disposable products. A slot is fixedly provided on the turntable, and the sampling tube, the nucleic acid transfer mechanism, and the chip are detachably installed in the slot. The driving device includes a rotation driving device, two rotating rods, and a docking driving device. The two rotating rods correspond to the first transfer valve and the second transfer valve, respectively. The docking driving device can drive the two rotating rods to approach and move away from the first transfer valve and the second transfer valve, respectively. When the two rotating rods approach the first transfer valve and the second transfer valve, they can be engaged with one side of the first transfer valve and the second transfer valve. The rotation driving device can independently drive the two rotating rods and drive the first transfer valve and the second transfer valve to rotate.

[0017] Preferably, the docking drive device includes a main motor, a transmission system, a rotating component, a shift fork, and a pull rod. The two rotating rods are rotatable and movable back and forth, respectively passing through two support sleeves. The same end of the two rotating rods faces the first transfer valve and the second transfer valve, respectively. The other end of each of the two rotating rods corresponds to the shift fork. The end of each rotating rod corresponding to the shift fork is provided with an annular first groove. The upper part of the shift fork is engaged in the first groove. The rotating component is rotatably mounted on the turntable. The main motor drives the rotating component to rotate through the transmission system. A connecting post is eccentrically provided on the top of the rotating component. The connecting post is connected to one end of the pull rod. The other end of the pull rod passes through the lower part of the shift fork and is fixedly provided with a limiting block at the end.

[0018] When the rotating component rotates in one direction, it can push the rotating rod toward the first transfer valve and the second transfer valve through the connecting column, the pull rod, the limiting block, and the shift fork, so that the two rotating rods respectively connect to one side of the first transfer valve and the second transfer valve;

[0019] When the rotating component rotates in another direction, it can pull the rotating rod away from the first transfer valve and the second transfer valve through the connecting column, the pull rod, the limiting block, and the shift fork, so that the end of the rotating rod is away from the first transfer valve and the second transfer valve.

[0020] Preferably, the sides of the first transfer valve and the second transfer valve are provided with cross grooves, and the ends of the rotating rod facing the first transfer valve and the second transfer valve are provided with cross structures;

[0021] Both of the rotating rods are fitted with a storage spring, one end of which can abut against the shift fork and the other end can abut against the limiting surface on the rotating rod;

[0022] During docking, the fork pushes the rotating rod to dock via the storage spring and compresses the storage spring to store force;

[0023] When the cross structure at the end of the rotating rod does not achieve accurate alignment with the side surfaces of the first and second transfer valves, the rotating drive device drives the rotating rod to rotate. When the cross structure is aligned with the cross slot, the rotating rod moves the cross structure into the cross slot under the elastic force of the storage spring.

[0024] Preferably, a displacement detection device is also provided. The displacement detection device detects whether the cross structure and the cross slot are properly aligned by detecting the position of the rotating rod. It can also transmit the alignment information of the cross structure and the cross slot to the control system. When the alignment is not properly aligned, the control system controls the rotation drive device to drive the rotating rod to rotate until the cross structure is aligned with the cross slot.

[0025] Preferably, the displacement detection device includes a spring sheet, the top of which is fixedly mounted on a support structure, and the bottom of which is opposite to the end of the rotating rod;

[0026] Under normal conditions, the end of the rotating rod abuts against one side of the spring piece, causing the spring piece to deform under pressure;

[0027] As the rotating rod moves toward the first transfer valve and the second transfer valve, the spring gradually returns to its original shape. After the rotating rod and the first transfer valve and the second transfer valve are connected, the end of the rotating rod disengages from the spring and is detected by the contact sensor.

[0028] Preferably, the rotary drive device includes a worm gear and a worm. The worm gear is mounted on the rotating rod, and the worm is matched with the worm gear. The worm is driven by the main motor, and a clutch is provided between the main motor and the worm. The main motor drives the rotating rod by disengaging and engaging the clutch.

[0029] The present invention achieves the following technical effects compared to the prior art:

[0030] In the air microbial detection system provided by this invention, the magnetic bead transfer process, magnetic bead elution process, and eluent transfer process are all driven by a drive device, eliminating the need for manual operation. Therefore, the solution provided by this invention improves the degree of automation and contributes to the realization of fully automated detection. Attached Figure Description

[0031] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0032] Figure 1 A schematic diagram of the integrated structure formed by the sampling tube, nucleic acid transfer mechanism, and chip;

[0033] Figure 2 for Figure 1 A sectional view;

[0034] Figure 3 This is a schematic diagram of the drive device.

[0035] Figure 4 This is a structural diagram of the docking drive device and the rotating rod;

[0036] Figure 5 A structural schematic diagram of the docking drive device, rotating rod, and integrated body;

[0037] Figure 6 A structural diagram showing the docking drive unit, card slot, integrated unit, and drive unit;

[0038] Figure 7 This is a schematic diagram of an automatic air microbial sampling and detection device that includes an air microbial detection system.

[0039] In the diagram: 11-Sampling tube; 111-Sampling chamber; 112-Air inlet; 113-Air outlet; 12-Nucleic acid transfer mechanism; 13-Chip; 131-Detection chamber; 132-Sample dispensing port; 121-First transfer valve; 122-Second transfer valve; 123-Magnetic rod chamber; 124-Receiving tank; 125-Eluting chamber; 126-First channel; 127-Second channel; 2-Drive device; 21-First motor; 22-Second motor; 23-Drive shaft; 24-Connecting rod; 25-Magnetic rod; 3-Docking drive device; 31-Transferring component; 32-Connecting column; 33-Pull rod; 34-Shift fork; 35-Worm gear; 36-Worm wheel; 41-Rotating rod; 42-Storage spring; 411-Cross structure; 5-Displacement detection device; 51-Spring; 61-Slot; 7-Turntable; 8-Frame. Detailed Implementation

[0040] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0041] The purpose of this invention is to provide an airborne microbial detection system to solve the problems existing in the prior art, reduce the degree of human intervention, improve the degree of automation, and contribute to the realization of fully automated detection.

[0042] To make the above-mentioned objects, features and advantages of the present invention more apparent and understandable, the present invention will be further described in detail below with reference to the accompanying drawings and specific embodiments.

[0043] This invention provides an airborne microbial detection system, such as... Figures 1 to 7 As shown, the system includes a sampling tube 11, a nucleic acid transfer mechanism 12, a chip 13, a drive device 2, and a control system (not shown in the figure; the control system can be an integrated chip or a computer; when the control system is a computer, it operates via wireless signal control). The sampling tube 11 contains a sampling chamber 111, which is pre-filled with magnetic beads and sampling liquid. The sampling tube 11 is used to sample microorganisms in the air. The nucleic acid transfer mechanism 12 contains an elution chamber 125, which is pre-filled with elution liquid. The sampling chamber 111 is connected to the elution chamber 125, and the elution chamber 125 is connected to the detection chamber 131 of the chip 13.

[0044] The control system controls the operation of the drive device 2. The drive device 2 can input the magnetic beads with nucleic acid adsorbed in the sampling chamber 111 into the elution chamber 125, and can also drive the magnetic beads to perform nucleic acid elution in the elution solution. After the nucleic acid elution is completed, the elution solution can also be input into the chip 13 for detection.

[0045] In the air microbial detection system provided by this invention, the magnetic bead transfer process, the magnetic bead elution process, and the eluent transfer process are all driven by the drive device 2, eliminating the need for manual operation. Therefore, the solution provided by this invention improves the degree of automation and contributes to the realization of fully automated detection.

[0046] In some embodiments, the airborne microbial detection system provided in this embodiment also includes a magnetic rod 25, a turntable drive device and a turntable; the sampling tube 11, the nucleic acid transfer mechanism 12 and the chip 13 are arranged sequentially and fixedly connected as a whole, and the three-in-one structure is called an integrated body.

[0047] The sampling chamber 111 and the elution chamber 125 are connected by a first channel 126 between them. A second channel 127 is provided on the side of the elution chamber 125 away from the first channel 126. The second channel 127 is connected to the sample inlet 132 of the detection chamber 131. A first transfer valve 121 and a second transfer valve 122 are respectively movably provided at the first channel 126 and the second channel 127. The first transfer valve 121 can close the first channel 126 and rotating the first transfer valve 121 can transfer the magnetic beads in the sampling chamber 111 to the elution chamber 125. The second transfer valve 122 can close the second channel 127 and rotating the second transfer valve 122 can transfer the eluent to the sample inlet 132.

[0048] The control system controls the drive device 2 to drive the first transfer valve 121 and the second transfer valve 122 to rotate. Both the first transfer valve 121 and the second transfer valve 122 are provided with magnetic rod cavities 123. The control system can control the drive device 2 to drive the magnetic rod 25 to be inserted into any one of the magnetic rod cavities 123.

[0049] The turntable is vertically mounted on the frame and can rotate on its own. The turntable drive device can drive the turntable to rotate. The sampling tube 11, chip 13, nucleic acid transfer mechanism 12, drive device 2 and magnetic rod 25 are all located on one side of the turntable.

[0050] In the sampling state, the integrated unit consisting of sampling tube 11, nucleic acid transfer mechanism 12 and chip 13 is in a vertical position; the sampling liquid in the vertical sampling tube 11 gathers at the bottom under the action of gravity, so that gas can impact the liquid surface to achieve air sampling.

[0051] During magnetic bead transfer and elution, to prevent the sampling liquid from flowing into the elution chamber 125 under gravity, the control system controls the turntable drive device to drive the turntable to rotate 90 degrees. The turntable drives the integrated body to rotate and make the integrated body horizontal. At this time, when the sampling liquid gathers at the bottom, the liquid level of the sampling liquid is lower than the channel opening of the first channel 126 to prevent the sampling liquid from flowing into the first channel 126. The drive device 2 drives the magnetic rod 25 to move the magnetic beads to realize magnetic bead transfer and elution.

[0052] Specifically, the surface of the first transfer valve 121 facing the sampling chamber 111 and the elution chamber 125 is recessed inward to form a receiving groove 124. In practice, when the integrated body is in a vertical state, the driving device 2 drives the magnetic rod 25 to be inserted into the magnetic rod cavity 123 of the first transfer valve 121. Under the action of magnetic force, the magnetic rod 25 adsorbs and fixes multiple magnetic beads in the receiving groove 124. At this time, the magnetic beads in the sampling chamber 111 can be transferred to the elution chamber 125 by driving the first transfer valve 121 to rotate 180 degrees through the driving device 2. During elution, the magnetic beads can be made to sway in the elution solution by rotating the turntable, or the magnetic rod 25 can be used to drive the magnetic beads to move back and forth in the elution solution to achieve elution.

[0053] To achieve automatic movement of the magnetic rod 25, such as Figure 3 As shown, in some embodiments, the drive device 2 includes a first motor 21, a second motor 22, a connecting rod 24, and a transmission shaft 23. The transmission shaft 23 is rotatably mounted on a turntable. Specifically, a support platform can be provided on the turntable, and the drive device 2 is rotatably mounted on the support platform via bearings. The first motor 21 is connected to one end of the transmission shaft 23. The second motor 22 is fixed on the transmission shaft 23, and the motor shaft of the second motor 22 is perpendicular to the transmission shaft 23. One end of the connecting rod 24 is fixed on the motor shaft of the second motor 22, and the other end extends toward one side of the elution chamber 125 and is fixed with a magnetic rod 25. The second motor 22 can drive the magnetic rod 25 to align with any magnetic rod cavity 123, and the first motor 21 can drive the magnetic rod 25 to be inserted into the magnetic rod cavity 123.

[0054] This embodiment uses two superimposed motors to achieve a complex magnetic bead transfer and elution process using a single magnetic rod 25. Of course, in other embodiments, two linear motors can be used to drive two magnetic rods 25 to perform linear motion to achieve one-to-one insertion of the magnetic rod 25 and the magnetic rod cavity 123, but this will inevitably increase the cost of the device.

[0055] In some embodiments, to further achieve automation, such as automated heating and image acquisition, the airborne microbial detection system provided in this embodiment also includes a heating mechanism and an image acquisition mechanism. The heating mechanism and the image acquisition mechanism are set together on a turntable. The chip 13 is made of transparent material. The heating mechanism includes a heating plate and a heating plate driving device. The heating plate is located at the bottom of the chip 13 and is rotatably mounted. Specifically, one end of the heating plate is fixedly mounted on a rotating shaft, which is connected to the heating plate driving device. The heating plate driving device can drive the heating plate to rotate. The image acquisition mechanism is located at the bottom of the chip 13. When the heating plate rotates to be close to the chip 13, it can heat the chip 13. After the heating plate has been heated to the reaction time, it rotates away from the chip 13. The heating plate will not block the chip 13. At this time, the image acquisition mechanism can acquire images of the detection results in the chip 13 and transmit them to a computer wirelessly or by wire, so that staff can obtain the detection results and perform analysis.

[0056] In some embodiments, the integrated assembly formed by the sampling tube 11, the nucleic acid transfer mechanism 12, and the chip 13 is a disposable product, to be replaced after one use. Therefore, it is necessary to make the structure driving the first transfer valve 121 and the second transfer valve 122 separable from the first transfer valve 121 and the second transfer valve 122 to achieve the purpose of replacing the sampling tube 11, the nucleic acid transfer mechanism 12, and the chip 13. Therefore, this embodiment makes the following improvements to the above embodiments:

[0057] In the air microbial detection system provided in this embodiment, a slot 61 is fixedly provided on the turntable. The integrated body is detachably installed in the slot 61. The driving device 2 includes a rotary driving device, two rotating rods 41 and a docking driving device 3. The two rotating rods 41 correspond to the first transfer valve 121 and the second transfer valve 122 respectively. The docking driving device 3 can drive the two rotating rods 41 to approach and move away from the first transfer valve 121 and the second transfer valve 122 respectively. When the two rotating rods 41 approach the first transfer valve 121 and the second transfer valve 122, they can be engaged with one side of the first transfer valve 121 and the second transfer valve 122. The rotary driving device can independently drive the two rotating rods 41 and drive the first transfer valve 121 and the second transfer valve 122 to rotate.

[0058] Among them, such as Figure 4 and Figure 5As shown, the docking drive device 3 includes a main motor, a transmission system, a rotating component 31, a shift fork 34, and a pull rod 33. Two rotating rods 41 are rotatable and can move back and forth, respectively passing through two support sleeves. The same end of the two rotating rods 41 faces the first transfer valve 121 and the second transfer valve 122, respectively. The other end of the two rotating rods 41 corresponds to the shift fork 34. The end of the rotating rod 41 corresponding to the shift fork 34 is provided with an annular first slot. The upper part of the shift fork 34 is engaged in the first slot. The rotating component 31 is rotatably mounted on the turntable. The main motor drives the rotating component 31 to rotate through the transmission system. A connecting post 32 is eccentrically mounted on the top of the rotating component 31. The connecting post 32 is connected to one end of the pull rod 33. The other end of the pull rod 33 passes through the lower part of the shift fork 34 and is fixedly provided with a limiting block at the end.

[0059] When the rotating component 31 rotates in one direction, it can push the rotating rod 41 toward the first transfer valve 121 and the second transfer valve 122 through the connecting column 32, the pull rod 33, the limit block, and the shift fork 34, so that the two rotating rods 41 are respectively connected to one side of the first transfer valve 121 and the second transfer valve 122.

[0060] When the rotating component 31 rotates in another direction, it can pull the rotating rod 41 away from the first transfer valve 121 and the second transfer valve 122 through the connecting column 32, the pull rod 33, the limit block, and the shift fork 34, so that the end of the rotating rod 41 is away from the first transfer valve 121 and the second transfer valve 122.

[0061] Therefore, this embodiment realizes the forward and backward movement of the two rotating rods 41, and the mechanism that drives the rotating rods 41 to move forward and backward will not affect the rotation drive device to drive the rotating rods 41 to rotate. Therefore, the solution provided by this embodiment facilitates the replacement of disposable integrated components.

[0062] In other embodiments, to achieve the forward and backward movement of the rotating rod 41, a linear motor can be used. The free-moving end of the linear motor is set as a ring-shaped drive plate. A ring-shaped groove is provided on the outer wall of the rotating rod 41 near the end. The end of the rotating rod 41 passes through the ring-shaped drive plate and the ring-shaped drive plate is locked in the ring-shaped groove. Under this structure, whether moving forward or backward, the ring-shaped drive plate can drive the rotating rod 41 to move forward and backward without affecting the rotation of the rotating rod 41. However, the linear motor in this embodiment can only achieve linear drive. The motor cannot be used as a power source for other structures. In contrast, in the scheme of using a main motor, rotating component 31, shift fork 34 and pull rod 33, the main motor can provide driving torque for multiple components.

[0063] In some embodiments, when the integrated structure is reusable, one end of the rotating rod 41 can be directly fixedly connected to the first transfer valve 121 and the second transfer valve 122 to achieve rotational drive without separation.

[0064] In some embodiments, the sides of the first transfer valve 121 and the second transfer valve 122 are provided with cross slots, and the end of the rotating rod 41 facing the first transfer valve 121 and the second transfer valve 122 is provided with a cross structure 411; the cross structure 411 matches the structure of the cross slot, and the cross structure 411 can be inserted into the cross slot when it moves toward the cross slot.

[0065] However, the probability of the cross structure 411 on the rotating rod 41 directly aligning with and locking into the cross slot when it moves toward the cross slot is relatively small. Therefore, in order to solve this problem, a storage spring 42 is sleeved on both rotating rods 41. One end of the storage spring 42 can abut against the shift fork 34, and the other end can abut against the limiting surface on the rotating rod 41.

[0066] During docking, the shift fork 34 pushes the rotating rod 41 to dock via the storage spring 42 and compresses the storage spring 42 to achieve power storage;

[0067] When the cross structure 411 at the end of the rotating rod 41 does not accurately align with the side surfaces of the first transfer valve 121 and the second transfer valve 122, the rotating drive device drives the rotating rod 41 to rotate. When the cross structure 411 is aligned with the cross slot, the rotating rod 41 moves the cross structure 411 into the cross slot under the elastic force of the storage spring 42.

[0068] In addition, the air microbial detection system is also equipped with a displacement detection device 5. The displacement detection device 5 detects whether the cross structure 411 and the cross slot are properly aligned by detecting the position of the rotating rod 41. It can also transmit the alignment information of the cross structure 411 and the cross slot to the control system. When the alignment is not properly aligned, the control system controls the rotation drive device to drive the rotating rod 41 to rotate until the cross structure 411 is aligned with the cross slot.

[0069] The displacement detection device 5 includes a spring piece 51, the top of which is fixedly mounted on a support structure, and the bottom of which is opposite to the end of the rotating rod 41.

[0070] Under normal conditions, the end of the rotating rod 41 abuts against one side of the spring piece 51, causing the spring piece 51 to deform under pressure;

[0071] As the rotating rod 41 moves toward the first transfer valve 121 and the second transfer valve 122, the spring piece 51 gradually returns to its original state. After the rotating rod 41 and the first transfer valve 121 and the second transfer valve 122 are connected, the end of the rotating rod 41 disengages from the spring piece 51 and is detected by the contact sensor.

[0072] In some embodiments, the rotary drive device includes a worm gear 36 and a worm 35. The worm gear 36 is mounted on the rotating rod 41, and the worm 35 is matched with the worm gear 36 for transmission. The worm 35 is driven by a main motor, and a clutch is provided between the main motor and the worm 35. The main motor drives the rotating rod 41 by disengaging and engaging the clutch.

[0073] The workflow is as follows:

[0074] In the initial state, the sampling tube 11 is in a vertical position, and the control system uses the sampling tube 11 to sample.

[0075] After sampling is complete, the following procedure will begin:

[0076] Step 1: The control system controls the turntable drive device to drive the turntable to rotate 90 degrees until the integrated body is horizontal.

[0077] Step 2: The driving device drives the magnetic rod 25 to be inserted into the magnetic rod cavity 123 of the first transfer valve 121 to attract the magnetic beads;

[0078] Specific step a: The second motor 22 drives the magnetic rod 25 to align with the magnetic rod cavity 123, and the first motor 21 drives the magnetic rod 25 to insert into the magnetic rod cavity 123.

[0079] Step 3: The drive device drives the first transfer valve 121 to rotate 180 degrees to transfer the magnetic beads into the elution chamber 125.

[0080] Specific step a: The clutch between the main motor and the rotating component 31 is engaged, and the main motor drives the rotating component 31 to rotate so as to push the two rotating rods 41 to be inserted into the first transfer valve 121 and the second transfer valve 122 respectively. The rotation drive device drives the rotating rods 41 to rotate and drives the first transfer valve 121 to rotate 180 degrees.

[0081] After the main motor drives the rotating rod 41 to move, the displacement detection device 5 detects that the position of the rotating rod 41 is not the docking position. The control system then controls the rotation drive device to drive the two rotating rods 41 to rotate a certain angle to achieve docking.

[0082] Step 4: The driving device drives the magnetic rods 25 to be alternately inserted into the two magnetic rod cavities 123 so as to realize the movement of the magnetic beads in the elution cavity 125 to realize the nucleic acid elution process.

[0083] Step 5: After washing is complete, the magnetic rod 25 is inserted into the magnetic rod cavity 123 of the first transfer valve 121 to fix the magnetic bead.

[0084] Step 6: The drive device drives the turntable to rotate until the integrated body is in a vertical position.

[0085] Step 7: The drive device drives the second transfer valve 122 to rotate 180 degrees to transfer the eluent in the elution chamber 125 to the chip 13 below.

[0086] Step 8: The heating plate driving device drives the heating plate to rotate until it is in close contact with the chip 13 to heat the chip 13.

[0087] Step 9: After the reaction is complete, the heating plate driving device drives the heating plate to rotate away from the chip 13.

[0088] Step 10: The image acquisition device acquires the image after the reaction within chip 13 and transmits it to the outside world.

[0089] Specific examples have been used to illustrate the principles and implementation methods of this invention. The descriptions of the above embodiments are only for the purpose of helping to understand the method and core ideas of this invention. Furthermore, those skilled in the art will recognize that, based on the ideas of this invention, there will be changes in the specific implementation methods and application scope. Therefore, the content of this specification should not be construed as a limitation of this invention.

Claims

1. An airborne microbial detection system, characterized in that: The system includes a sampling tube, a nucleic acid transfer mechanism, a chip, a driving device, a magnetic rod, a turntable driving device, a turntable, and a control system. The sampling tube has a sampling chamber, which is pre-filled with magnetic beads and sampling liquid. The sampling tube is used to sample microorganisms in the air. The nucleic acid transfer mechanism has an elution chamber. The elution chamber is pre-filled with elution solution, the sampling chamber is connected to the elution chamber, and the elution chamber is connected to the detection chamber of the chip. The turntable is vertically mounted on the frame and can rotate on its own. The turntable driving device can drive the turntable to rotate. The sampling tube, the chip, the nucleic acid transfer mechanism, the driving device and the magnetic rod are all located on one side of the turntable. In the sampling state, the integrated unit consisting of the sampling tube, the nucleic acid transfer mechanism, and the chip is in a vertical position. During the transfer and elution of magnetic beads, the turntable drives the integrated body to rotate and makes the integrated body horizontal. The sampling tube, the nucleic acid transfer mechanism, and the chip are all disposable products. A slot is fixedly provided on the turntable, and the sampling tube, the nucleic acid transfer mechanism, and the chip can be detachably installed in the slot. The driving device includes a rotation driving device, two rotating rods, and a docking driving device. The two rotating rods correspond to the first transfer valve and the second transfer valve, respectively. The docking driving device can drive the two rotating rods to approach and move away from the first transfer valve and the second transfer valve, respectively. When the two rotating rods approach the first transfer valve and the second transfer valve, they can be engaged with one side of the first transfer valve and the second transfer valve. The rotation driving device can independently drive the two rotating rods and drive the first transfer valve and the second transfer valve to rotate. The driving device includes a first motor, a second motor, a connecting rod, and a transmission shaft. The transmission shaft is rotatably mounted on the turntable. The first motor is connected to one end of the transmission shaft. The second motor is fixed on the transmission shaft, and the motor shaft of the second motor is perpendicular to the transmission shaft. One end of the connecting rod is fixed on the motor shaft of the second motor, and the other end extends toward one side of the elution chamber and is fixed with the magnetic rod. The second motor can drive the magnetic rod to align with any one of the magnetic rod cavities, and the first motor can drive the magnetic rod to be inserted into the magnetic rod cavity. The docking drive device includes a main motor, a transmission system, a rotating component, a shift fork, and a pull rod. The two rotating rods are rotatable and movable back and forth, respectively passing through two support sleeves. The same end of the two rotating rods faces the first transfer valve and the second transfer valve, respectively. The other end of each of the two rotating rods corresponds to the shift fork. The end of each rotating rod corresponding to the shift fork is provided with an annular first groove. The upper part of the shift fork is engaged in the first groove. The rotating component is rotatably mounted on the turntable. The main motor drives the rotating component to rotate through the transmission system. A connecting post is eccentrically provided on the top of the rotating component. The connecting post is connected to one end of the pull rod. The other end of the pull rod passes through the lower part of the shift fork and is fixedly provided with a limiting block at the end. When the rotating component rotates in one direction, it can push the rotating rod toward the first transfer valve and the second transfer valve through the connecting column, the pull rod, the limiting block, and the shift fork, so that the two rotating rods respectively connect to one side of the first transfer valve and the second transfer valve; When the rotating component rotates in another direction, it can pull the rotating rod away from the first transfer valve and the second transfer valve through the connecting column, the pull rod, the limiting block, and the shift fork, so that the end of the rotating rod is away from the first transfer valve and the second transfer valve; The control system controls the operation of the driving device, which can input magnetic beads with nucleic acid adsorbed in the sampling chamber into the elution chamber, and can also drive the magnetic beads to perform nucleic acid elution in the elution solution. After the nucleic acid elution is completed, the elution solution can also be input into the chip for detection.

2. The airborne microbial detection system according to claim 1, characterized in that: The sampling tube, the nucleic acid transfer mechanism, and the chip are arranged in sequence; The sampling chamber and the elution chamber are connected by a first channel between them; a second channel is provided on the side of the elution chamber away from the first channel, and the second channel is connected to the sample inlet of the detection chamber. A first transfer valve and a second transfer valve are respectively movably arranged at the first channel and the second channel. The first transfer valve can close the first channel and rotating the first transfer valve can transfer the magnetic beads in the sampling chamber to the elution chamber. The second transfer valve can close the second channel and rotating the second transfer valve can transfer the eluent to the sample inlet. The control system controls the driving device to drive the first transfer valve and the second transfer valve to rotate. Both the first transfer valve and the second transfer valve are provided with magnetic rod cavities. The control system can control the driving device to drive the magnetic rod to be inserted into any one of the magnetic rod cavities.

3. The airborne microbial detection system according to claim 2, characterized in that: It also includes a heating mechanism and an image acquisition mechanism. The chip is made of a transparent material. The heating mechanism includes a heating plate and a heating plate driving device. The heating plate is located at the bottom of the chip and is rotatably mounted. The heating plate driving device can drive the heating plate to rotate. The image acquisition mechanism is located at the bottom of the chip. When the heating plate rotates to be close to the chip, it can heat the chip. When the heating plate rotates away from the chip, the image acquisition mechanism can acquire images of the detection results in the chip.

4. The airborne microbial detection system according to claim 1, characterized in that: The first transfer valve and the second transfer valve are provided with cross grooves on their sides, and the end of the rotating rod facing the first transfer valve and the second transfer valve is provided with a cross structure; Both of the rotating rods are fitted with a storage spring, one end of which can abut against the shift fork and the other end can abut against the limiting surface on the rotating rod; During docking, the fork pushes the rotating rod to dock via the storage spring and compresses the storage spring to store force; When the cross structure at the end of the rotating rod does not achieve accurate alignment with the side surfaces of the first and second transfer valves, the rotating drive device drives the rotating rod to rotate. When the cross structure is aligned with the cross slot, the rotating rod moves the cross structure into the cross slot under the elastic force of the storage spring.

5. The airborne microbial detection system according to claim 4, characterized in that: A displacement detection device is also provided. The displacement detection device detects whether the cross structure and the cross slot are properly aligned by detecting the position of the rotating rod. It can also transmit the alignment information of the cross structure and the cross slot to the control system. When the alignment is not properly aligned, the control system controls the rotation drive device to drive the rotating rod to rotate until the cross structure is aligned with the cross slot.

6. The airborne microbial detection system according to claim 5, characterized in that: The displacement detection device includes a spring sheet, the top of which is fixedly mounted on a support structure, and the bottom of which is opposite to the end of the rotating rod. Under normal conditions, the end of the rotating rod abuts against one side of the spring piece, causing the spring piece to deform under pressure; As the rotating rod moves toward the first transfer valve and the second transfer valve, the spring gradually returns to its original shape. After the rotating rod and the first transfer valve and the second transfer valve are connected, the end of the rotating rod disengages from the spring and is detected by the contact sensor.

7. The airborne microbial detection system according to claim 4, characterized in that: The rotary drive device includes a worm gear and a worm. The worm gear is mounted on the rotating rod, and the worm is matched with the worm gear. The worm is driven by the main motor, and a clutch is provided between the main motor and the worm. The main motor drives the rotating rod by disengaging and engaging the clutch.

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