一种可进行细胞分选、粒子捕获等操作的新型数字微流控芯片及其制作方法

By combining a hydrophobic layer and dielectric wetting force in the photoelectric tweezers functional area, the problem of existing digital microfluidic chips being unable to achieve high-throughput droplet manipulation and fine manipulation of tiny substances is solved, enabling efficient parallel experiments and precise control of the chip.

CN116764705BActive Publication Date: 2026-07-17BEIJING INST OF TECH

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
BEIJING INST OF TECH
Filing Date
2023-06-29
Publication Date
2026-07-17

AI Technical Summary

Technical Problem

Existing digital microfluidic chips are difficult to achieve high-throughput droplet manipulation and precise manipulation of tiny substances within microdroplets on the same chip, and photoelectric tweezers chips are easily damaged and have high operating voltage requirements.

Method used

A novel digital microfluidic chip was designed, which separates the photoelectric tweezers functional area from the digital microfluidic functional area through an isolation structure. A hydrophobic layer is set in the photoelectric tweezers functional area, and the chip is manipulated by combining dielectric wetting force and photoelectrophoresis force to avoid photoconductive layer breakdown.

Benefits of technology

This enables high-throughput parallel operation of microdroplets and fine manipulation of tiny substances within microdroplets on the same chip, improving chip durability and operational efficiency.

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Abstract

本发明公开了一种可进行细胞分选、粒子捕获等操作的新型数字微流控芯片及其制作方法。本发明提供了两种结构的数字微流控芯片:一种是采用微泵驱动的微流道连接光电镊功能区和数字微流控功能区,分别在光电镊区域的上极板与数字微流控区域的上极板打孔,利用微流道与微泵实现微液滴在两个功能区的转移;一种是将数字微流控芯片特定区域划分为光电镊功能区,在进行介电层生长时遮蔽该区域。本发明数字微流控芯片集成了光电镊技术对微小物体精准、高通量并行操控以及数字微流控技术对微液滴进行高通量并行操作的优势,解决了光电镊等微操作技术无法操控微小物体所处液体环境以及常规数字微流控技术难以实现对液滴内微小物质进行精准操控的问题。
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