A stem cell filtration separation device

Abstract

The application relates to the technical field of cell separation devices, and particularly discloses a stem cell filtering and separating device, which comprises a test table, a base fixed to the lower end of the test table, a closed door arranged on the front of the test table, a cell extraction cavity arranged in the test table, two centrifugal bins symmetrically arranged in the cell extraction cavity, a rotating mechanism arranged on the outer side of the centrifugal bins, a stem cell storage mechanism fixed between the centrifugal bins, a centrifugal liquid pipeline arranged between the stem cell storage mechanism and the centrifugal bins, a waste liquid bin arranged at the lower end of the centrifugal bin and arranged in the base. Through the arrangement of the centrifugal liquid pipeline, the centrifugal bins can be communicated with each other, stem cells separated out can be sucked into another centrifugal bin for secondary centrifugation after one of the centrifugal bins completes centrifugation, and the stem cell extraction efficiency is improved.

Description

Technical Field

[0001] This invention relates to the field of cell separation device technology, and in particular to a stem cell filtration and separation device. Background Technology

[0002] Cell separation techniques include centrifugation, flow cytometry, and electrophoresis. Centrifugation is a fundamental method for studying cell nuclei, mitochondria, Golgi apparatus, lysosomes, microbodies, and various macromolecules. Flow cytometry is a technique for rapid quantitative analysis and sorting of single cells. Electrophoresis refers to the migration of cells with a net positive or negative charge on their surface under the influence of an external electric field at a certain pH value. Centrifugation is the most commonly used cell separation technique. However, existing biological cell separation devices have drawbacks. During the separation process, some dust and bacteria may be present in the air inside the device. When centrifuged, these dust and bacteria adhere to the biological cells, ultimately contaminating the separated cell fluid and organelles, rendering them unusable. Furthermore, the centrifugation process requires manual judgment of the degree of centrifugation, resulting in poor centrifugation efficiency.

[0003] Chinese patent CN115722350A discloses a biological cell separation device, specifically relating to the field of cell separation. It includes a protective shell, a rotating shaft, and a sealing cover. The rotating shaft is hinged to one side of the protective shell, and the sealing cover is fixedly connected to the outer wall of the rotating shaft. A transmission column is rotatably connected inside the protective shell, and a separation filter is fixedly connected to the outer wall of the transmission column. A rotary motor is rotatably connected to the bottom of the transmission column, and a rotating plate is fixedly connected to the top of the transmission column. A movable push column is fixedly connected to the top of the rotating plate, and a movable component is slidably connected to the outer wall of the movable push column. A movable squeezing rod is fixedly connected to one side of the movable component. This invention, by incorporating the movable component and piston box, allows the transmission column to drive the movable push column to rotate via the rotating plate during cell separation, ultimately completely removing air from the separation device and solving the problem of dust and bacteria contaminating cell fluid and organelles during biological cell separation.

[0004] While the above invention has solved the problems in the background art to some extent, some problems still exist:

[0005] 1. This device separates cells through a filter, but the filtered cells become entangled with the filter, making them difficult to separate, difficult to clean, and impractical.

[0006] 2. This device can only be set to one centrifugation speed and cannot centrifuge the cell solution to be separated in stages. Each time, the cells in the filter must be removed before the next centrifugation can be performed, resulting in low separation efficiency.

[0007] To address these issues, we propose a stem cell filtration and separation device. Summary of the Invention

[0008] The purpose of this invention is to provide a stem cell filtration and separation device to solve the problems mentioned in the background art, such as cells being entangled with the filter screen, making them difficult to separate, difficult to clean, impractical, unable to be centrifuged in stages, requiring the cells in the filter screen to be removed before the next centrifugation, resulting in low separation efficiency.

[0009] To achieve the above objectives, the present invention provides the following technical solution:

[0010] A stem cell filtration and separation device includes an experimental table with a base fixed to its lower end. Support feet are installed at the four corners of the lower end of the base. A closed door is provided on the front of the experimental table. A cell extraction chamber is provided inside the experimental table. Two centrifuge chambers are symmetrically installed inside the cell extraction chamber. A rotating mechanism is installed on the outside of the centrifuge chambers and is fixed to the inner wall of the cell extraction chamber. A stem cell storage mechanism is fixed between the centrifuge chambers. A centrifugal fluid pipeline is provided between the stem cell storage mechanism and the centrifuge chambers. A waste liquid tank is provided at the lower end of the centrifuge chambers and is located inside the base.

[0011] The centrifuge chamber includes a centrifuge shell. A main inlet pipe is rotatably connected to the upper end of the centrifuge shell via a bearing. Sealing rings are installed at the upper and lower ends of the bearing. A cleaning assembly is fixed to the outside of the main inlet pipe. An electric heating ring is provided on the inner wall of the side of the centrifuge shell. A through hole is provided at the lower end of the centrifuge shell, and a bottom-blocking ring is installed at the through hole. A support connecting plate is installed at the lower end of the bottom-blocking ring. A telescopic cylinder is fixed on the support connecting plate. One end of the telescopic cylinder is fixedly connected to the support connecting plate, and the other end is fixedly connected to the lower end of the centrifuge shell.

[0012] In a further embodiment, the experimental platform is provided with several placement platforms on the front, a lighting lamp is installed on the right end of each placement platform, a material inlet is provided on the upper wall of the experimental platform, a chute is provided on the right side of the material inlet, a sealing plate is installed in the chute, the sealing plate is slidably connected to the chute, and cell fluid inlets are provided on the left and right sides of the material inlet.

[0013] In a further embodiment, the main inlet pipe includes a main pipe, which is rotatably connected to the centrifuge chamber via a bearing. An electric telescopic pipe is fixed to the lower end of the main pipe, and an end pipe is installed at the lower end of the electric telescopic pipe. A multi-directional nozzle is provided at the bottom of the end pipe.

[0014] In a further embodiment, the cleaning assembly includes a support plate fixed to the outer side of the upper end of the main pipe, a telescopic cylinder II fixed to the lower end of the support plate, a mounting plate fixed to the end of the telescopic cylinder II, a first electromagnet fixed to the mounting plate, and a cleaning plate fixed to the end of the first electromagnet via a fixing plate.

[0015] In a further embodiment, the rotating mechanism includes a fixed ring with a circular groove inside. The lower end of the fixed ring is fixed to the lower inner wall of the cell extraction chamber by a support column. A gear ring is provided inside the fixed ring and fixed to the middle of the outer side of the centrifuge chamber. Evenly distributed fixing rods are provided at the upper and lower ends of the gear ring. Ball bearings are provided on the fixing rods and are in rolling connection with the circular groove. A pinion is meshed with the gear ring, and a drive motor is installed at the upper end of the pinion. The drive motor is fixed to the inner side wall of the cell extraction chamber by a motor fixing plate.

[0016] In a further embodiment, the stem cell storage mechanism includes a circular seat fixed to the lower inner wall of the cell extraction chamber. A telescopic hydraulic cylinder is fixed to the upper end of the circular seat, and a fixing component is installed on the upper end of the telescopic hydraulic cylinder. A liquid storage tank is fixed inside the fixing component.

[0017] In a further embodiment, the fixing component includes a fixing shell, a second electromagnet fixed on the fixing shell, a movable clamp fixed on the second electromagnet, a fixing clamp provided on the side wall of the fixing shell, the fixing clamp being fixed at a symmetrical position to the movable clamp, and a washer provided on the lower inner wall of the fixing shell.

[0018] In a further embodiment, the centrifugal liquid pipeline includes a main feed pipe, which is installed at the upper end of the main inlet pipe. A feed trough is installed at the upper end of the main feed pipe. An extraction pipe is provided on the side of the main feed pipe. A first water pump is installed on the extraction pipe. A first feed pipe is provided at the rear end of the main feed pipe. A water inlet valve is provided on the first feed pipe. A water storage tank is installed at the rear end of the first feed pipe. A connecting pipe is installed on the side of the first feed pipe. A connecting pump is provided on the connecting pipe.

[0019] In a further embodiment, the bottom-blocking ring is in the shape of an inverted cone, and its surface is uniformly provided with four guide grooves.

[0020] In a further embodiment, a discharge ring is provided on the lower inner wall of the cell extraction chamber, the discharge ring is located directly below the centrifuge chamber, and a waste liquid chamber is provided at the lower end of the discharge ring.

[0021] Compared with the prior art, the beneficial effects of this invention are as follows: This stem cell filtration and separation device is equipped with two centrifuge chambers, which can be interconnected through centrifugal fluid pipelines. After centrifugation in one centrifuge chamber, the separated stem cells can be drawn into the other centrifuge chamber for secondary centrifugation, improving the stem cell extraction efficiency. Furthermore, a cleaning component is installed inside the centrifuge chamber, which automatically cleans the chamber after each centrifugation, preventing residual substances from the previous centrifugation from affecting the next operation. A rotating mechanism is also included to provide rotational power to the centrifuge chamber, making centrifugation more stable. Specifically:

[0022] 1. In this invention, two symmetrically distributed centrifuge chambers are set in the cell extraction chamber. A main inlet pipe is set in the centrifuge chamber through a bearing, which can extract the centrifuged cell fluid and inject it into the other centrifuge chamber through the main inlet pipe, connecting pipe and connecting pump for secondary centrifugation. Repeating this step can completely separate the stem cells with high concentration and good separation effect. During the separation process, the sealed centrifuge shell and the heating coil can create a temperature environment suitable for maintaining the activity of stem cells and improve the separation effect.

[0023] 2. In this invention, by setting a cleaning component inside the centrifuge chamber, the centrifuge chamber can be automatically cleaned after each centrifugation, avoiding the impact of residual substances from the previous centrifugation on the next centrifugation operation. The cleaning component includes a support plate, which is fixed to the outer side of the upper end of the main pipe. The height of the first electromagnet and the cleaning plate is controlled by the telescopic cylinder 2. During cleaning, the first electromagnet is activated, so that the cleaning plate contacts the inner wall of the centrifuge chamber. When the rotating mechanism drives the centrifuge chamber to rotate, the cleaning plate can scrape off the cell fluid residue attached to the inner wall of the centrifuge chamber. At the same time, the water inlet valve is opened to inject clean water, and the telescopic cylinder 2 is controlled to move up and down to make the cleaning more thorough.

[0024] 3. In this invention, a rotating mechanism is provided in the cell extraction chamber, which provides rotational power to the centrifuge chamber while maintaining structural stability and making centrifugation more stable. The rotating mechanism includes a fixed ring with a circular groove inside. The circular groove is connected to a ball bearing in a rolling manner, making the rotation of the centrifuge chamber more stable and smooth. When the rotating mechanism is working, it provides power through a drive motor, which drives the pinion to rotate. The pinion meshes with the gear ring, transmitting power to the gear ring, and finally driving the centrifuge chamber to rotate at high speed, resulting in good centrifugation effect. Attached Figure Description

[0025] Figure 1 This is a schematic diagram of a stem cell filtration and separation device.

[0026] Figure 2 This is a schematic diagram of the internal structure of the stem cell filtration and separation device in this invention;

[0027] Figure 3 This is a schematic diagram of the centrifuge chamber, rotating mechanism, and centrifugal liquid pipeline in this invention;

[0028] Figure 4 This is a schematic diagram of the bottom sealing ring in this invention;

[0029] Figure 5 This is a schematic diagram of the rotating mechanism in this invention;

[0030] Figure 6 In this invention Figure 5 Enlarged structural diagram at point A in the middle;

[0031] Figure 7 This is a schematic diagram of the internal structure of the centrifuge chamber in this invention;

[0032] Figure 8 This is a schematic diagram of the fixing component in this invention;

[0033] Figure 9 In this invention Figure 7 Enlarged structural diagram at point B.

[0034] In the diagram: 1. Experimental table; 11. Storage platform; 12. Lighting lamp; 13. Feed inlet; 14. Slide chute; 15. Sealing plate; 16. Cell fluid inlet; 17. Cell extraction chamber; 171. Discharge ring; 2. Base; 3. Support leg; 4. Sealing door; 5. Centrifuge chamber; 51. Centrifuge shell; 52. Main inlet pipe; 521. Main pipe; 522. Electric telescopic pipe; 523. End pipe; 524. Multi-directional nozzle; 53. Bearing; 54. Sealing ring; 55. Cleaning assembly; 551. Support plate; 552. Telescopic cylinder II; 553. Mounting plate; 554. First electromagnet; 555. Fixing plate; 556. Cleaning plate; 56. Heating coil; 57. Bottom sealing ring; 571. Feed guide chute; 58. Telescopic cylinder I 59. Support connecting plate; 6. Rotating mechanism; 61. Fixing ring; 611. Circular slide groove; 62. Support column; 63. Gear ring; 64. Fixing rod; 65. Ball bearing; 66. Pinion; 67. Drive motor; 68. Motor fixing plate; 7. Stem cell storage mechanism; 71. Circular seat; 72. Telescopic cylinder three; 73. Fixing component; 731. Fixing shell; 732. Second electromagnet; 733. Washer; 734. Moving clamp; 735. Fixing clamp; 74. Storage tank; 8. Centrifugal liquid pipeline; 81. Main feed pipe; 82. Feed trough; 83. Extraction pipe; 84. First water pump; 85. First feed pipe; 86. Water inlet valve; 87. Water storage tank; 88. Connecting pipe; 89. Connecting pump; 9. Waste liquid tank. Detailed Implementation

[0035] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0036] Please see Figure 1 and Figure 2In this invention, a stem cell filtration and separation device includes an experimental table 1, which serves as a platform for operators to place experimental equipment. A hollow base 2 is fixed to the lower end of the experimental table 1, providing connection and support. Support feet 3 are installed at the four corners of the lower end of the base 2 to prevent it from contacting the ground and facilitate cleaning. A closed door 4 is provided on the front of the experimental table 1, with transparent glass for easy observation of the interior. The closed door 4 can be opened and closed freely. When the centrifuge chamber 5 malfunctions, the closed door 4 can be opened for easy repair. Inside the experimental table 1 is a cell extraction chamber 17, with two symmetrically installed centrifuge chambers 5 for centrifuging the cells to be separated. A rotating mechanism 6 is installed on the outside of the centrifuge chamber 5. The rotating mechanism 6 is fixed on the inner wall of the cell extraction chamber 17. The rotating mechanism 6 is used to provide power to drive the centrifuge chamber 5 to rotate at high speed. A stem cell storage mechanism 7 is fixed between the centrifuge chambers 5. The stem cell storage mechanism 7 is used to store the stem cells after separation. A centrifuge fluid pipeline 8 is set between the stem cell storage mechanism 7 and the centrifuge chamber 5. The centrifuge fluid pipeline 8 is used to add cell fluid, inject water, and realize the communication between the two centrifuge chambers 5. A waste liquid tank 9 is set at the lower end of the centrifuge chamber 5. The waste liquid tank 9 is set in the base 2 and is used to store waste liquid. A discharge ring 171 is set on the inner wall of the lower end of the cell extraction chamber 17. The discharge ring 171 is set directly below the centrifuge chamber 5. The waste liquid tank 9 is set at the lower end of the discharge ring 171.

[0037] Please see Figure 1 and Figure 2 The experimental table 1 has several shelves 11 on its front side, which can store some commonly used experimental instruments. A lamp 12 is installed on the right end of the shelf 11 to provide illumination. The upper wall of the experimental table 1 has a material inlet 13. After the stem cells are separated, they are taken out through the material inlet 13. A chute 14 is provided on the right side of the material inlet 13. A sealing plate 15 is installed in the chute 14. The sealing plate 15 is slidably connected to the chute 14. When taking out the material, the sealing plate 15 is slid to the right along the chute 14 to expose the material inlet 13. After taking out the material, the sealing plate 15 is slid to the left along the chute 14 to close the material inlet 13. Cell fluid inlets 16 are provided on the left and right sides of the material inlet 13. The cell fluid inlets 16 are used to add the cell fluid to be separated to the centrifuge chamber 5.

[0038] Based on the above structural features, when this invention is in operation, the cell fluid to be separated is first injected into the centrifuge chamber 5 through the cell fluid inlet 16. The rotating mechanism 6 is started to drive the centrifuge chamber 5 to rotate at high speed to complete the initial centrifugation. After centrifugation, the initially separated cell fluid is extracted through the centrifuge fluid pipeline 8 and injected into the centrifuge chamber 5 on the other side for secondary centrifugation. The remaining waste liquid is discharged into the waste liquid chamber 9. The above steps are repeated multiple times to centrifuge the cell fluid to be separated multiple times, and finally high concentration of stem cells are separated. The stem cells are then injected into the stem cell storage mechanism 7 again through the centrifuge fluid pipeline 8. The sealing plate 15 is opened and the stem cells are taken out from the feeding port 13. After the operation is completed, the centrifuge chamber 5 can be automatically cleaned to avoid the residual substances from the previous operation from affecting the next centrifugation operation.

[0039] Please see Figure 2 , Figure 3 , Figure 4 , Figure 7 and Figure 9 The centrifuge chamber 5 includes a centrifuge shell 51, which holds the cell fluid to be centrifuged. A main inlet pipe 52 is rotatably connected to the upper end of the centrifuge shell 51 via a bearing 53. The main inlet pipe 52 is used to add or remove liquid from the centrifuge shell 51. Sealing rings 54 are installed at the upper and lower ends of the bearing 53 to prevent outside air from entering the centrifuge shell 51 and contaminating the cell fluid. A cleaning assembly 55 is fixed to the outside of the main inlet pipe 52 for cleaning the centrifuge shell 51 after centrifugation. An electric heating coil 56 is installed on the inner wall of the side of the centrifuge shell 51 to create a suitable temperature environment inside the centrifuge shell 51. In a suitable temperature environment, the lower end of the centrifuge shell 51 is provided with a through hole, and a bottom-blocking ring 57 is installed at the through hole. The bottom-blocking ring 57 is used to discharge waste liquid. A support connecting plate 59 is installed at the lower end of the bottom-blocking ring 57. The support connecting plate 59 is used for support connection. A telescopic cylinder 58 is fixed on the support connecting plate 59. One end of the telescopic cylinder 58 is fixedly connected to the support connecting plate 59, and the other end is fixedly connected to the lower end of the centrifuge shell 51. When the telescopic cylinder 58 retracts, the bottom-blocking ring 57 is in close contact with the centrifuge shell 51, sealing the centrifuge shell 51. When the telescopic cylinder 58 extends, the bottom-blocking ring 57 is disengaged from the centrifuge shell 51, and the waste liquid is discharged.

[0040] Please see Figure 7 The main inlet pipe 52 includes a main pipe 521, which provides support and guides the liquid into the centrifuge housing 51. The main pipe 521 is rotatably connected to the centrifuge chamber 5 via a bearing 53. An electric telescopic pipe 522 is fixed at the lower end of the main pipe 521. An end pipe 523 is installed at the lower end of the electric telescopic pipe 522. The electric telescopic pipe 522 can extend and retract, thereby controlling the height of the end pipe 523. A multi-directional nozzle 524 is provided at the bottom of the end pipe 523. The multi-directional nozzle 524 can spray cell fluid evenly into the centrifuge housing 51.

[0041] Please see Figure 7 and Figure 9 The cleaning assembly 55 includes a support plate 551, which is fixed to the outer side of the upper end of the main pipe 521 and serves as a fixing device. A telescopic cylinder 552 is fixed to the lower end of the support plate 551, and an installation plate 553 is fixed to the end of the telescopic cylinder 552. A first electromagnet 554 is fixed on the installation plate 553, and a cleaning plate 556 is fixed to the end of the first electromagnet 554 through the fixing plate 555. After each centrifugation, the centrifuge chamber 5 can be automatically cleaned to prevent the residue from the previous centrifugation from affecting the next centrifugation operation. During cleaning, the first electromagnet 554 is activated to make the cleaning plate 556 contact the inner wall of the centrifuge chamber 5. When the rotating mechanism 6 drives the centrifuge chamber 5 to rotate, the cleaning plate 556 can scrape off the cell fluid residue attached to the inner wall of the centrifuge chamber 5.

[0042] Please see Figure 4 The bottom sealing ring 57 is in the shape of an inverted cone, and four guide grooves 571 are evenly arranged on its surface, so that the waste liquid can flow into the waste liquid tank 9 along the guide grooves 571;

[0043] Based on the above structural features, when the present invention is in operation, it is equipped with two centrifuge chambers 5, which can be interconnected through centrifugal fluid pipelines 8. After one centrifuge chamber 5 has completed centrifugation, the separated stem cells can be drawn into the other centrifuge chamber 5 for secondary centrifugation, which improves the stem cell extraction efficiency. In addition, a cleaning component 55 is provided in the centrifuge chamber 5, which can automatically clean the centrifuge chamber 5 after each centrifugation to avoid the residual substances from the previous centrifugation affecting the next centrifugation operation.

[0044] Please see Figure 2 , Figure 5 and Figure 6 The rotating mechanism 6 includes a fixed ring 61 with a circular groove 611 inside. The lower end of the fixed ring 61 is fixed to the lower inner wall of the cell extraction chamber 17 by a support column 62, providing support and fixation. A toothed ring 63 is provided inside the fixed ring 61 and is fixed to the middle of the outer side of the centrifuge chamber 5. Evenly distributed fixing rods 64 are provided at the upper and lower ends of the toothed ring 63, providing support. Ball bearings 65 are provided on the fixing rods 64, and the ball bearings 65 are in rolling connection with the circular groove 611. The ball bearing 65 acts as a limit switch, rolling within the circular groove 611 to ensure smoother rotation of the centrifuge chamber 5. The gear ring 63 meshes with a pinion 66, and a drive motor 67 is mounted on the upper end of the pinion 66. The drive motor 67 is fixed to the inner wall of the cell extraction chamber 17 via a motor mounting plate 68. During operation, the drive motor 67 is started, driving the pinion 66 to rotate. The pinion 66 meshes with the gear ring 63, transmitting power to the gear ring 63, ultimately driving the centrifuge chamber 5 to rotate at high speed. This results in good centrifugation effect, a simple structure, and strong stability.

[0045] In accordance with the above structural features, when the present invention is in operation, a rotating mechanism 6 is provided in the cell extraction chamber 17 to provide rotational power to the centrifuge chamber 5 while maintaining structural stability and making centrifugation more stable. The rotating mechanism 6 includes a fixed ring 61, and a circular groove 611 is provided in the fixed ring 61. The circular groove 611 is connected to the ball bearing 65 in a rolling manner, making the rotation of the centrifuge chamber 5 more stable and smooth.

[0046] Please see Figure 2 , Figure 3 and Figure 8 The stem cell storage mechanism 7 includes a circular seat 71, which is fixed to the lower inner wall of the cell extraction chamber 17. The circular seat 71 provides support and fixation. A telescopic hydraulic cylinder 3 72 is fixed to the upper end of the circular seat 71. The telescopic hydraulic cylinder 3 72 can extend and retract freely, thereby adjusting the height of the fixing component 73 and the liquid storage tank 74 to facilitate the extraction of separated stem cells. The fixing component 73 is installed on the upper end of the telescopic hydraulic cylinder 3 72. The liquid storage tank 74 is fixed inside the fixing component 73. The fixing component 73 is used to fix the liquid storage tank 74, which is easy to pick up and has good flexibility. The liquid storage tank 74 stores separated stem cells.

[0047] Please see Figure 8 The fixing component 73 includes a fixing shell 731, which provides support and fixation. A second electromagnet 732 is fixed on the fixing shell 731. When the second electromagnet 732 is energized, it retracts; when it is de-energized, it extends. A movable clamp 734 is fixed on the second electromagnet 732. A fixing clamp 735 is provided on the side wall of the fixing shell 731 and is fixed symmetrically to the movable clamp 734. A washer 733 is provided on the lower inner wall of the fixing shell 731. The washer 733 provides shock absorption and is used to protect the liquid storage tank 74. When the liquid storage tank 74 is fixed, the second electromagnet 732 is energized. At this time, the movable clamp 734 retracts with the second electromagnet 732. After the liquid storage tank 74 is placed on the washer 733, the second electromagnet 732 is de-energized. At this time, the second electromagnet 732 extends, which drives the movable clamp 734 to cooperate with the fixing clamp 735 to clamp the liquid storage tank 74.

[0048] In accordance with the above structural features, when the present invention is in operation, the separated stem cell tissue fluid is sent into the storage tank 74 through the centrifugal fluid pipeline 8. When extraction is required, the sealing plate 15 is opened, the telescopic cylinder 3 72 is extended, and the storage tank 74 is sent out from the dispensing port 13 for easy retrieval.

[0049] Please see Figure 3The centrifuge fluid pipeline 8 includes a main feed pipe 81, which serves as an intermediate transition. The main feed pipe 81 is installed at the upper end of the main liquid inlet pipe 52. A feed trough 82 is installed at the upper end of the main feed pipe 81 for adding the cell fluid to be separated. An extraction pipe 83 is provided on the side of the main feed pipe 81. A first water pump 84 is installed on the extraction pipe 83. The separated stem cells can be introduced into the storage tank 74 through the extraction pipe 83 and the first water pump 84. A first feed pipe 85 is provided at the rear end of the main feed pipe 81. A water inlet valve 86 is provided on the first feed pipe 85. A water storage tank 87 is installed at the rear end of the first feed pipe 85. When it is necessary to clean the centrifuge chamber 5, the water inlet valve 86 is opened, and water is injected into the centrifuge chamber 5 through the first feed pipe 85 and the water storage tank 87. A connecting pipe 88 is installed on the side of the first feed pipe 85. A connecting pump 89 is provided on the connecting pipe 88.

[0050] Based on the above structural features, when the present invention is in operation, it uses the main feed pipe 81, connecting pipe 88 and connecting pump 89 to inject into the centrifuge chamber 5 on the other side for secondary centrifugation. By repeating this step, stem cells can be completely separated with high concentration and good separation effect.

[0051] The working principle of this invention is as follows: During operation, the cell fluid to be separated is first injected into the centrifuge chamber 5 through the cell fluid inlet 16. The rotating mechanism 6 is started to drive the centrifuge chamber 5 to rotate at high speed to complete the initial centrifugation. After centrifugation, the initially separated cell fluid is extracted through the centrifuge fluid pipeline 8 and injected into the centrifuge chamber 5 on the other side for secondary centrifugation. The remaining waste liquid is discharged into the waste liquid chamber 9. The above steps are repeated multiple times to centrifuge the cell fluid to be separated multiple times, and finally high concentration of stem cells are separated. The stem cells are then injected into the stem cell storage mechanism 7 again through the centrifuge fluid pipeline 8. The sealing plate 15 is opened and the stem cells are taken out from the feeding port 13. After the operation is completed, the centrifuge chamber 5 can be automatically cleaned to avoid the residual substances from the previous operation from affecting the next centrifugation operation.

[0052] In operation, the centrifuge chamber 5 has a centrifuge shell 51 that holds the cell solution to be centrifuged. A main inlet pipe 52 is rotatably connected to the upper end of the centrifuge shell 51 via a bearing 53. The main inlet pipe 52 is used to add or remove liquid from the centrifuge shell 51. Sealing rings 54 are installed at the upper and lower ends of the bearing 53 to prevent outside air from entering the centrifuge shell 51 and contaminating the cell solution. A cleaning assembly 55 is fixed to the outside of the main inlet pipe 52 for cleaning the centrifuge shell 51 after centrifugation. An electric heating coil 56 is installed on the inner wall of the side of the centrifuge shell 51 to create a suitable temperature inside the centrifuge shell 51. In a suitable environment, the lower end of the centrifuge shell 51 is provided with a through hole, and a bottom-blocking ring 57 is installed at the through hole. The bottom-blocking ring 57 is used to discharge waste liquid. A support connecting plate 59 is installed at the lower end of the bottom-blocking ring 57. The support connecting plate 59 is used for support connection. A telescopic cylinder 58 is fixed on the support connecting plate 59. One end of the telescopic cylinder 58 is fixedly connected to the support connecting plate 59, and the other end is fixedly connected to the lower end of the centrifuge shell 51. When the telescopic cylinder 58 retracts, the bottom-blocking ring 57 is in close contact with the centrifuge shell 51, sealing the centrifuge shell 51. When the telescopic cylinder 58 extends, the bottom-blocking ring 57 disengages from the centrifuge shell 51, and the waste liquid is discharged.

[0053] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that the invention can be implemented in other specific forms without departing from its spirit or essential characteristics. Therefore, the embodiments should be considered in all respects as exemplary and non-limiting, and the scope of the invention is defined by the appended claims rather than the foregoing description. Thus, all variations falling within the meaning and scope of equivalents of the claims are intended to be included within the present invention. No reference numerals in the claims should be construed as limiting the scope of the claims.

[0054] Furthermore, it should be understood that although this specification describes embodiments, not every embodiment contains only one independent technical solution. This narrative style is merely for clarity. Those skilled in the art should consider the specification as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.

Claims

1. A stem cell filtration and separation device, characterized in that, The experimental table (1) is fixed with a base (2) at the lower end of the experimental table (1). Support feet (3) are installed at the four corners of the lower end of the base (2). A closed door (4) is provided on the front of the experimental table (1). A cell extraction chamber (17) is provided inside the experimental table (1). Two centrifuge chambers (5) are symmetrically installed inside the cell extraction chamber (17). A rotating mechanism (6) is installed on the outside of the centrifuge chambers (5). The rotating mechanism (6) is fixed on the inner wall of the cell extraction chamber (17). A stem cell storage mechanism (7) is fixed between the centrifuge chambers (5). A centrifugal fluid pipeline (8) is provided between the stem cell storage mechanism (7) and the centrifuge chambers (5). A waste liquid tank (9) is provided at the lower end of the centrifuge chambers (5). The waste liquid tank (9) is located inside the base (2). The centrifuge chamber (5) includes a centrifuge shell (51). The upper end of the centrifuge shell (51) is rotatably connected to a main inlet pipe (52) via a bearing (53). A sealing ring (54) is installed at the upper and lower ends of the bearing (53). A cleaning component (55) is fixed on the outside of the main inlet pipe (52). An electric heating ring (56) is provided on the inner wall of the side of the centrifuge shell (51). A through hole is provided at the lower end of the centrifuge shell (51), and a bottom-blocking ring (57) is installed at the through hole. A support connecting plate (59) is installed at the lower end of the bottom-blocking ring (57). A telescopic cylinder (58) is fixed on the support connecting plate (59). One end of the telescopic cylinder (58) is fixedly connected to the support connecting plate (59), and the other end is fixedly connected to the lower end of the centrifuge shell (51). The centrifugal liquid pipeline (8) includes a main feed pipe (81), which is installed at the upper end of the main liquid inlet pipe (52). A feed trough (82) is installed at the upper end of the main feed pipe (81). An extraction pipe (83) is provided on the side of the main feed pipe (81). A first water pump (84) is installed on the extraction pipe (83). A first feed pipe (85) is provided at the rear end of the main feed pipe (81). A water inlet valve (86) is provided on the first feed pipe (85). A water storage tank (87) is installed at the rear end of the first feed pipe (85). A connecting pipe (88) is installed on the side of the first feed pipe (85). A connecting pump (89) is provided on the connecting pipe (88).

2. The stem cell filtration and separation device according to claim 1, characterized in that, The experimental table (1) has several platforms (11) on its front side. A lighting lamp (12) is installed on the right end of each platform (11). A material inlet (13) is provided on the upper wall of the experimental table (1). A chute (14) is provided on the right side of the material inlet (13). A sealing plate (15) is installed in the chute (14). The sealing plate (15) is slidably connected to the chute (14). Cell fluid inlets (16) are provided on the left and right sides of the material inlet (13).

3. The stem cell filtration and separation device according to claim 1, characterized in that, The main inlet pipe (52) includes a main pipe (521), which is rotatably connected to the centrifuge chamber (5) via a bearing (53). An electric telescopic pipe (522) is fixed at the lower end of the main pipe (521), and an end pipe (523) is installed at the lower end of the electric telescopic pipe (522). A multi-directional nozzle (524) is provided at the bottom of the end pipe (523).

4. The stem cell filtration and separation device according to claim 3, characterized in that, The cleaning assembly (55) includes a support plate (551), which is fixed to the outer side of the upper end of the main pipe (521). A telescopic cylinder (552) is fixed to the lower end of the support plate (551), and an installation plate (553) is fixed to the end of the telescopic cylinder (552). A first electromagnet (554) is fixed on the installation plate (553), and a cleaning plate (556) is fixed to the end of the first electromagnet (554) through a fixing plate (555).

5. The stem cell filtration and separation device according to claim 1, characterized in that, The rotating mechanism (6) includes a fixed ring (61), a circular groove (611) is provided in the fixed ring (61), the lower end of the fixed ring (61) is fixed to the lower inner wall of the cell extraction chamber (17) by a support column (62), a gear ring (63) is provided in the fixed ring (61), the gear ring (63) is fixed to the middle of the outer side of the centrifuge chamber (5), the upper and lower ends of the gear ring (63) are provided with evenly distributed fixing rods (64), the fixing rods (64) are provided with ball bearings (65), the ball bearings (65) are rolled in connection with the circular groove (611), the gear ring (63) is meshed with a small gear (66), the upper end of the small gear (66) is equipped with a drive motor (67), the drive motor (67) is fixed to the inner side wall of the cell extraction chamber (17) by a motor fixing plate (68).

6. The stem cell filtration and separation device according to claim 1, characterized in that, The stem cell storage mechanism (7) includes a circular seat (71), which is fixed on the lower inner wall of the cell extraction chamber (17). A telescopic cylinder three (72) is fixed at the upper end of the circular seat (71), and a fixing component (73) is installed at the upper end of the telescopic cylinder three (72). A liquid storage tank (74) is fixed inside the fixing component (73).

7. The stem cell filtration and separation device according to claim 6, characterized in that, The fixing component (73) includes a fixing shell (731), a second electromagnet (732) is fixed on the fixing shell (731), a movable clamp (734) is fixed on the second electromagnet (732), a fixing clamp (735) is provided on the side wall of the fixing shell (731), the fixing clamp (735) is fixed at a symmetrical position to the movable clamp (734), and a washer (733) is provided on the lower inner wall of the fixing shell (731).

8. The stem cell filtration and separation device according to claim 1, characterized in that, The bottom-blocking ring (57) is in the shape of an inverted cone, and four guide grooves (571) are evenly arranged on its surface.

9. The stem cell filtration and separation device according to claim 1, characterized in that, The cell extraction chamber (17) has a discharge ring (171) on the inner wall at the lower end. The discharge ring (171) is located directly below the centrifuge chamber (5). The discharge ring (171) has a waste liquid chamber (9) at its lower end.

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