Optical signal detection device, gel unit, and method for manufacturing gel unit

Abstract

The present application provides a light signal detection device, a gel unit, and a manufacturing method of the gel unit, which make the processing of the gel easy. The gel unit is used for mounting on an objective lens provided in the light signal detection device, and the gel unit is characterized by comprising a gel and an auxiliary member, the auxiliary member comprising a first surface and an opening, the diameter of the opening being smaller than the outer diameter of the gel, the first surface facing the gel, a part of the first surface being fixed relative to the bottom surface of the gel by adhesion, and the center of the bottom surface not being in contact with the auxiliary member.

Description

Technical Field

[0001] This invention relates to an optical signal detection device, a gel unit, and a method for manufacturing the gel unit. Background Technology

[0002] In recent years, research using cell aggregates such as spheroids and organoids, obtained by collecting large numbers of cells and culturing them in 3D, has attracted attention. Such samples are, for example, 100 μm to 500 μm in size.

[0003] In deep cellular observation of such samples, liquid immersion objectives are generally used. In liquid immersion objectives, a higher numerical aperture than that of dry objectives can be achieved by filling the space between the objective and the sample (more precisely, the holding component that holds the sample) with immersion liquid.

[0004] By using an immersion liquid with a refractive index close to that of the sample, spherical aberration caused by refractive index mismatch at the interface between the sample and the immersion liquid (air in the case of a dry objective lens) can also be suppressed. As described in Patent Document 1, the deeper the observation position, the more significant the effect of spherical aberration caused by refractive index mismatch becomes. Therefore, by suppressing spherical aberration, deeper positions can be observed.

[0005] Existing technical documents

[0006] Patent documents

[0007] Patent Document 1: Japanese Patent Application Publication No. 2017-026666 Summary of the Invention

[0008] The problem that the invention aims to solve

[0009] When using liquid immersion, maintenance and operation can sometimes impose a burden on users. Therefore, the use of easily handled gel immersion is considered. Furthermore, for example, to mount a gel onto an objective lens, a method of directly contacting the gel with tweezers or similar tools is considered. However, sometimes the gel is difficult to remove from the tweezers, and the gel can be damaged during handling. Therefore, a technique is desired that can easily mount the gel onto the objective lens while minimizing gel damage is required.

[0010] In one aspect, the object of the present invention is to make the processing of gels easier.

[0011] Methods for solving problems

[0012] One aspect of the present invention provides a gel unit for mounting on an objective lens of an optical signal detection device. The gel unit is characterized by having a gel and an auxiliary component. The auxiliary component has a first surface and an opening. The diameter of the opening is smaller than the outer diameter of the gel. The first surface faces the gel. A portion of the first surface is fixed relative to the bottom surface of the gel by an adhesive force. The center of the bottom surface does not contact the auxiliary component.

[0013] Invention Effects

[0014] It makes handling gels easier. Attached Figure Description

[0015] Figure 1 This is a diagram of an exemplary gel representing an implementation method.

[0016] Figure 2 This is a diagram illustrating an auxiliary component in an embodiment of a covered objective lens method.

[0017] Figure 3 This is a diagram illustrating the installation of the gel onto the auxiliary component in an exemplary embodiment.

[0018] Figure 4 This diagram illustrates the installation of a gel with a plastic cap onto an auxiliary component.

[0019] Figure 5 This is an example of the installation of a gel-to-auxiliary component using a mold with a fitting part.

[0020] Figure 6 This is a diagram illustrating the mounting of an auxiliary component to the front lens frame of the objective lens in an exemplary embodiment.

[0021] Figure 7 This is a diagram illustrating another auxiliary component of the implementation method.

[0022] Figure 8 This is a diagram illustrating the mounting of an auxiliary component to the front lens frame of the objective lens in an exemplary embodiment.

[0023] Figure 9 This is a diagram illustrating a sheet-like auxiliary component in an embodiment.

[0024] Figure 10 This is a diagram illustrating the installation of auxiliary components onto the objective lens in an illustrative embodiment.

[0025] Figure 11 This diagram illustrates an example of an auxiliary component using an elastomer in an implementation method.

[0026] Figure 12 This is a diagram illustrating the installation of auxiliary components onto the objective lens in an illustrative embodiment.

[0027] Figure 13This is a diagram illustrating an example of a method for attaching gel to an auxiliary component according to an embodiment.

[0028] Figure 14 This is a diagram illustrating another example of a method for installing gel onto an auxiliary component according to an embodiment.

[0029] Figure 15 This is a diagram illustrating a sheet-like auxiliary component in an embodiment.

[0030] Figure 16 This is a diagram illustrating the installation of auxiliary components onto the objective lens in an illustrative embodiment.

[0031] Figure 17 This is a diagram illustrating an embodiment of a method for attaching gel to an auxiliary component.

[0032] Figure 18 This is a diagram illustrating another example of a method for installing gel onto an auxiliary component according to an embodiment.

[0033] Figure 19 This is a diagram illustrating an auxiliary component consisting of multiple parts in an embodiment.

[0034] Figure 20 This is a diagram illustrating the installation of auxiliary components onto the objective lens in an illustrative embodiment.

[0035] Figure 21 This is a diagram illustrating the configuration of the gel towards the auxiliary component.

[0036] Figure 22 This is a diagram illustrating an installation system for attaching an execution aid component to a gel, as shown in an exemplary embodiment.

[0037] Figure 23 This is a diagram illustrating the hardware structure of a computer in an illustrative implementation.

[0038] Figure 24 This is a diagram illustrating the structure of a microscope apparatus according to one embodiment.

[0039] Figure 25 This is another diagram illustrating one embodiment of the gel.

[0040] Figure 26 This is another diagram illustrating one embodiment of the gel.

[0041] Figure 27 This is another diagram illustrating one embodiment of the gel.

[0042] Label Explanation

[0043] 1: Microscope setup

[0044] 2: Light source

[0045] 3: Objective lens

[0046] 4: Converter

[0047] 5: Camera

[0048] 11: Auxiliary components

[0049] 101: Gel

[0050] 200: Auxiliary components

[0051] 201: First Page

[0052] 202: Second Page

[0053] 203: Opening

[0054] 210: Axis

[0055] 2200: Install System

[0056] 2201: Computer

[0057] 2202: Platform Controller

[0058] 2203: Support Controller

[0059] 2204: Filming Device

[0060] 2205: Installation device

[0061] 2210: Configuration Components

[0062] 2211: Platform

[0063] 2212: Electric support column

[0064] 2213: Cage

[0065] 2230: Auxiliary components

[0066] 2250: Mold

[0067] 2301: Processor

[0068] 2302: Memory

[0069] 2303: Storage device

[0070] 2304: Reading device

[0071] 2305: Removable storage media

[0072] 2306: Communication Interface

[0073] 2307: Input / Output Interface

[0074] 2308: Bus

[0075] C: Container

[0076] S: Sample Detailed Implementation

[0077] Hereinafter, several embodiments of the present invention will be described in detail with reference to the accompanying drawings. Furthermore, in the various drawings, corresponding elements are labeled with the same reference numerals.

[0078] As mentioned above, the use of immersion liquid can sometimes impose additional burdens on users in terms of maintenance and operation.

[0079] For example, in actual observation, dry objectives and immersion objectives are sometimes switched during the observation process. A typical example is the use of a dry objective with lower magnification and a wider field of view to identify the area of ​​interest, followed by the use of a higher-resolution immersion objective to observe the area of ​​interest in detail.

[0080] However, when switching from a dry objective to an immersion objective, a fresh supply of immersion liquid is required to fill the space between the objective and the sample. Furthermore, when switching from an immersion objective to a dry objective, the immersion liquid must be reliably wiped off the sample surface without leaving any residue. Thus, in addition to the objective switching itself, various additional operations are needed during the switching between dry and immersion objectives. Consequently, observation is temporarily interrupted, making smooth observation difficult.

[0081] Furthermore, when observing samples using only immersion objectives, various issues arise compared to using dry objectives. For example, when using water as the immersion solution, it evaporates during prolonged observation. Therefore, it is necessary to replenish the immersion solution appropriately during observation. Additionally, using oil as the immersion solution presents challenges such as difficult cleaning and the tendency to generate bubbles due to its high viscosity. Moreover, when using objectives with long working distances in an inverted microscope, it is difficult to maintain the immersion solution between the objective and the sample using surface tension. Maintaining the immersion solution through surface tension is also difficult when observing the sample from an inclined or lateral direction, presenting the same challenges as when using objectives with long working distances. Therefore, in these cases, a large-scale device is needed to maintain the immersion solution between the objective and the sample. Furthermore, when observing over a wide range in the depth direction, the distance between the objective and the sample changes significantly during observation, leading to the problem of immersion solution easily overflowing between the objective and the sample.

[0082] Thus, the current situation is that using liquid immersion objectives places a greater burden on users compared to using dry objectives.

[0083] Therefore, the use of easily handled gel impregnation is considered. Furthermore, for example, to mount the gel onto the objective lens, a method of directly contacting the gel with tweezers or the like is considered. However, the gel is difficult to detach from the tweezers, and sometimes it is damaged during handling. Therefore, a technique is desired that can easily mount the gel onto the objective lens while suppressing damage to the gel.

[0084] Therefore, in the embodiments described below, an auxiliary component that adheres closely to the gel is used to mount the gel onto the objective lens. Since the auxiliary component is used to handle the gel, the operation becomes easier, and damage to the gel is also prevented. The embodiments are described in more detail below.

[0085] Figure 1 This is a diagram illustrating an exemplary gel of an implementation method. In Figure 1 , exemplified Figure 1 (a) and Figure 1 (b) consists of two gels.

[0086] Figure 1 The gel 101 in (a) is, for example, made of silicone with a consistency of 69. Furthermore, the gel's holding member side (specimen side) is spherical with a radius of curvature of 2 mm. The holding member is configured, for example, to hold the sample between the objective lens and the sample when the gel is placed in a microscope. On the other hand, the gel's auxiliary member side (objective lens side) is conical with a circular base and an outer diameter of Φ10 mm (base angle 42°).

[0087] Figure 1 (b) The gel 101 is, for example, made of silicone with a consistency of 69. Furthermore, the gel's holding component side (specimen side) is spherical with a radius of curvature of 1.69 mm. On the other hand, the gel's auxiliary component side (objective side) is conical with a circular base and an outer diameter of Φ9 mm (base angle 45°).

[0088] In one embodiment, the center of the bottom surface of gel 101 can be shaped along the shape of the front end face of the objective lens. For example, if the front end face of the objective lens is planar, the center of the bottom surface of gel 101 can be planar; if the front end face of the objective lens is spherical, the center of the bottom surface of gel 101 can be spherical. Furthermore, the shape of the outer edge region (the region near the outer edge) extending from the center of the bottom surface of gel 101 towards the outer edge can be shaped along the shape of the front end face of the objective lens. When there is continuity between the center of the bottom surface of gel 101 and the boundary of the outer edge region of gel 101, the outer edge region of gel 101 is the same as the center of gel 101 and can be considered the bottom surface of gel 101. For example, even if there are slight steps between the center of the bottom surface of gel 101 and the boundary of the outer edge region of gel 101, as long as they are considered to be the same surface, the outer edge region of gel 101 is the same as the center of gel 101 and can be considered the bottom surface of gel 101. However, if the position of the outer edge region of gel 101 differs significantly from the center position of the bottom surface of gel 101, making them not essentially the same, then the outer edge region of gel 101 cannot be considered the bottom surface of gel 101. For example, it is also possible to design the outer edge region of gel 101 as extending along the side of the objective lens. In this case, the outer edge region of gel 101 cannot be considered the bottom surface of gel 101. If gel 101 with such an outer edge region is in direct contact with the side of the objective lens (in a state where the auxiliary component is not between gel 101 and the objective lens), gel 101 is difficult to detach from the objective lens, and gel 101 may remain in close contact with the objective lens frame, etc. As an example, gel 101 is shaped to cover the frame component of the objective lens from the front end of the objective lens. In this case, the contact area between gel 101 and the objective lens increases. Moreover, if the contact area increases, the corresponding adhesion force between gel 101 and the objective lens increases relative to the adhesion force between gel 101 and the auxiliary component. As a result, it is sometimes difficult to remove gel 101 from the objective lens.

[0089] Furthermore, for example, the outer edge region of the bottom surface of gel 101 and the auxiliary component are fixed. In one example, the auxiliary component may be fixed relative to gel 101 along the bottom surface of gel 101. Additionally, the bottom surface of gel 101 may be, for example, a surface extending from the center of the bottom surface of the gel. Furthermore, the bottom surface of gel 101 may be a surface of gel 101 opposite to the opening of the auxiliary component, or may include a surface opposite to the opening of the auxiliary component. Additionally, the bottom surface of gel 101 may be a surface in contact with the front end surface (lens surface) of the objective lens.

[0090] Furthermore, the shape of gel 101 is not limited to the shapes described above; it can also be other shapes. Other examples of gel 101 include, for instance... Figures 25 to 27 This will be discussed later.

[0091] in addition, Figure 1 Gel 101 is used, for example, in observations using lenses with an objective lens of 40X, NA 0.8, WD 3.18 mm, an effective diameter at the lens tip of Φ 5 mm, and a lens diameter of Φ 6.8 mm.

[0092] Next, examples of auxiliary components used for placing the gel on the objective lens will be described. Furthermore, the gel used below will be... Figure 1 The mountain-shaped gel 101 shown is used to illustrate an example of an auxiliary component. However, the implementation is not limited to this, and the gel may also be in other shapes.

[0093] Figure 2 This is a diagram illustrating an auxiliary component 200 covering the objective lens in an exemplary embodiment. Figure 2 A cross-section of the auxiliary component 200 is shown in (a). Additionally, in Figure 2 Figure (b) shows a diagram representing the upper surface of the auxiliary component 200. Figure 2 In (a), axis 210 indicates the position of the optical axis when the auxiliary component 200 is mounted on the objective lens. The auxiliary component 200 is made of a material such as a metal like aluminum or brass, a rigid body like resin or ceramic. The auxiliary component 200 has a first surface 201 on which the gel is disposed, a second surface 202 facing the frame component of the objective lens, and an opening 203.

[0094] In addition, Figure 2 The illustrated implementation example demonstrates the use of Figure 1 In the case of gel 101 with shape (b), the auxiliary component 200 has an opening diameter 203 smaller than the outer diameter of the gel (e.g., the diameter of the bottom surface is 9 mm), for example, 7 mm. Additionally, the opening diameter 203 of the auxiliary component 200 is larger than the effective diameter of the objective lens. A first surface 201 of the auxiliary component 200 faces the gel 101, and a portion of the first surface 201 is adhesive, fixed relative to the gel 101 by an adhesive force. The first surface 201 includes the opening 203. Furthermore, the outer diameter of the first surface 201 is larger than the outer diameter of the gel (e.g., the diameter of the bottom surface is 9 mm), for example, 12 mm.

[0095] Figure 3 This is a diagram illustrating the installation of gel 101 onto auxiliary component 200 according to an exemplary embodiment. Figure 3 (a) shows a mold 301 for gel 101. Components of the mold 301 for gel 101 can be made of materials with good release properties, such as ABS (acrylonitrile, butadiene, styrene) resin, polyethylene, polypropylene, or fluoropolymer. In another example, a silicone or fluorinated release agent may be applied to the surface of the mold components for gel 101.

[0096] Additionally, gel 101 is installed on auxiliary component 200, for example, in the following order.

[0097] Step 1: Inject the pre-gelling liquid into the gel mold.

[0098] Process 2: such as Figure 3 The auxiliary component 200 is placed as shown in (a).

[0099] Step 3: Allow it to gel and solidify under gel-curing conditions.

[0100] Step 4: While maintaining close contact with auxiliary component 200, pull gel 101 out of mold 301. Figure 3 (b)

[0101] Furthermore, the mold 301 of gel 101 may include, for example, markings indicating the positions for placing the auxiliary component 200. Thus, in step 2, for example, by placing the auxiliary component 200 on the mold 301 of gel 101 in accordance with the markings, alignment of gel 101 and auxiliary component 200 can be achieved.

[0102] Furthermore, in step 4, for example, by configuring the auxiliary component 200 to have a higher adhesion force with the gel 101 than the adhesion force between the mold 301 and the gel 101, the gel 101 can be attached to the auxiliary component 200 and pulled out from the mold 301. Figure 3 In (b), a gel unit 300 is shown obtained by mounting gel 101 on auxiliary component 200.

[0103] Alternatively, a plastic cap (CAP) can be used to install the gel 101 onto the auxiliary component 200. Figure 4 This diagram illustrates the installation of gel 101 with a plastic cap onto auxiliary component 200. Figure 4 In this example, gel 101 is installed on auxiliary component 200 according to the following steps. Furthermore, plastic cap 400 is manufactured to match the shape of auxiliary component 200. And, the portion of plastic cap 400 containing the front end 401 of gel 101 forms the shape of a mold for gel 101.

[0104] Step 1: The pre-gelling liquid is cured at the front end 401 of the plastic cap 400. Figure 4 (a)

[0105] Step 2: After gelation, cover the auxiliary component 200 with the gel-coated plastic cap 400, and install the gel 101 onto the auxiliary component 200. Figure 4 (b)

[0106] Step 3: Remove the plastic cap 400.

[0107] Compared to the front end 401 of the mold that forms the gel 101 in the plastic cap 400, the auxiliary component 200 has a stronger adhesion to the gel 101, thus allowing the gel 101 to be installed on the auxiliary component 200 and removed from the plastic cap 400. The installation of the gel 101 from the plastic cap to the auxiliary component 200 can also be performed with the auxiliary component 200 installed in the objective lens.

[0108] Alternatively, the plastic cap can be made from materials with good release properties, such as polyethylene, polypropylene, or fluoropolymers. Or, a silicone or fluoropolymer release agent can be applied to the surface of the mold for the plastic cap.

[0109] Alternatively, in the installation of gel 101 onto auxiliary component 200, a mold 500 with fitting portion 501 can be used, which is shaped to fit the shape of auxiliary component 200. Figure 5 This diagram illustrates the mounting of gel 101 onto auxiliary component 200 using a mold 500 with fitting portion 501. Furthermore, the mold 500 is manufactured to match the shape of the auxiliary component 200.

[0110] exist Figure 5 In the example, gel 101 is installed on auxiliary component 200, for example, according to the following steps.

[0111] Step 1: Use a mold 500 to solidify the pre-gelled liquid.

[0112] Step 2: After gelation, the mold with gel is placed over the auxiliary component 200 to install the gel onto the auxiliary component.

[0113] Step 3: Remove the mold 500.

[0114] Furthermore, the installation of gel 101 from the mold to the auxiliary component 200 can also be performed with the auxiliary component 200 mounted on the objective lens. Since the auxiliary component 200 has a stronger adhesion to the gel than the mold 500, the gel can be attached to the auxiliary component 200 and then pulled out of the mold 500.

[0115] Next, the mounting of the gel 101 to the objective lens using the auxiliary component 200 is illustrated. Figure 6 This is a diagram illustrating the mounting of the auxiliary component 200 to the front lens frame of the objective lens in an exemplary embodiment. Figure 6 Image (a) shows the front end portion 600 of the objective lens. The front end portion 600 includes, for example, a front lens 601 and a lens support frame 602.

[0116] Moreover, in Figure 6In (b), an auxiliary component 200 is provided in the front end portion 600. For example, the shape of the auxiliary component 200 can be made to match the shape of the objective lens to cover the objective lens as the mounting destination.

[0117] Additionally, at least one of the auxiliary component 200 and the objective lens may include a fixing portion for securing the auxiliary component 200 to the objective lens. The fixing portion may, for example, be a shape or mechanism for securing the auxiliary component 200 to the objective lens.

[0118] For example, in Figure 6 In region 610 of (b), the auxiliary component 200 and the lens support frame 602 can be formed as a fixing part with threaded teeth and threaded grooves. The fixing part may have, for example, threaded teeth and threaded grooves with a diameter of 14.4 mm and a pitch of 0.5 mm. The auxiliary component 200 is configured such that, when screwed into the objective lens and stopped, the gel 101 is in close contact with the front end face of the objective lens. In this case, if the thread is rotated in the opposite direction, the auxiliary component 200 and the gel 101 can be separated from the objective lens.

[0119] Furthermore, the fixing part is not limited to a screw-in threaded connection; other fixing methods are also possible. For example, in another embodiment, a hook can be used to fix the auxiliary component 200 to the front end portion 600 of the objective lens, such as the front lens 601 or the lens support frame 602. If a surface engagement method is also used in the hook method, an elastic body, even if it is not a rigid body, can be installed as an auxiliary component.

[0120] Furthermore, in one embodiment, the gel 101 can be positioned on the auxiliary component 200 such that the center of the opening 203 of the auxiliary component 200 is aligned with the central axis of the gel 101 disposed on the auxiliary component 200 within a predetermined error range. Additionally, when mounting the gel 101 onto the objective lens using the auxiliary component 200, the auxiliary component 200, the objective lens, and the gel 101 can be positioned such that the optical axis of the objective lens is aligned with the central axis of the gel 101 within a predetermined error range. Figure 6 (b) shows the optical axis 620 of the objective lens.

[0121] In addition, the mounting position of the auxiliary component 200 to the objective lens can be various, and the shape of the auxiliary component 200 can be made to match the shape of the objective lens at the mounting position.

[0122] Figure 7 This is a diagram illustrating another auxiliary component 700 in an illustrative embodiment. Figure 7 In (a), for example, a cross-section of the auxiliary component 700 is shown. Additionally, in Figure 7Figure (b) shows the upper surface of the auxiliary component 700. The auxiliary component 700 is made of a rigid material such as metal, resin, or ceramic. The auxiliary component 700 has a first surface 701 on which the gel 101 is disposed, a second surface 702 facing the frame component of the objective lens, and an opening 703.

[0123] In addition, Figure 7 The illustrated implementation example demonstrates the use of Figure 1 In the case of gel 101 with shape (b), the auxiliary component 700 has an opening diameter 703 smaller than the outer diameter of the gel (e.g., the diameter of the bottom surface is 9 mm), for example, 7 mm. Additionally, the opening diameter 703 of the auxiliary component 700 is larger than the effective diameter of the objective lens. A first surface 701 of the auxiliary component 700 faces the gel 101, and a portion of the first surface 701 is fixed relative to the gel 101 by an adhesive force. The first surface 701 includes the opening 703. Furthermore, the outer diameter of the first surface 701 is larger than the outer diameter of the gel (e.g., the diameter of the bottom surface is 9 mm), for example, 14 mm.

[0124] Next, the mounting of the gel 101 to the objective lens using the auxiliary component 700 is illustrated. Figure 8 This is a diagram illustrating the mounting of the auxiliary component 700 to the front lens frame of the objective lens in an exemplary embodiment. Figure 8 (a) shows the front end portion 800 of the objective lens. The front end portion 800 includes a front lens 801, a lens support frame 802, a main body 803 for housing the lens support frame 802, and a lens 804.

[0125] Furthermore, in Figure 8 In (b), an auxiliary component 700 is provided in the front end portion 800. For example, the shape of the auxiliary component 700 can be made to match the shape of the objective lens to cover the objective lens as the mounting destination.

[0126] Additionally, at least one of the auxiliary component 700 and the objective lens may include a fixing portion for securing the auxiliary component 700 to the objective lens. The fixing portion may, for example, be a shape or mechanism for securing the auxiliary component 700 to the objective lens.

[0127] For example, in Figure 8 In region 810 of (b), a thread can be formed on one of the auxiliary component 700 and the front end portion 800, and a threaded groove can be formed on the other as a fixing part. The fixing part may have a diameter of 19 mm and a pitch of 0.5 mm, for example. The auxiliary component 700 is configured such that, in the stopped position when screwed into the objective lens, the gel 101 is in close contact with the front end face of the objective lens. In this case, if the thread is rotated in the opposite direction, the auxiliary component 700 and the gel 101 can be separated from the objective lens.

[0128] Furthermore, the fixing part is not limited to a screw-in threaded connection; other fixing methods are also possible. For example, in another embodiment, a hook can be used to fix the auxiliary part 700 to the front end portion 800. If a face-fitting member is also used in the hook method, an elastic body, even if it is not a rigid body, can be installed as an auxiliary part.

[0129] Furthermore, the inner diameter of the fixing portion 810 of the auxiliary component 700 used for mounting to the objective lens body 803 is, for example, 19 mm, and the pitch is 0.5 mm. The outer diameter of the objective lens body 803 is... Figure 6 The lens support frame 602 of the illustrated objective lens front end portion 600 has a larger outer diameter; therefore, the inner diameter of the region 810 of the fixing portion of the auxiliary component 700 is larger than that of the lens support frame 602. Figure 6 The inner diameter of the fixing part of the auxiliary component 200 in region 610 is large.

[0130] In another embodiment, the gel 101 can be disposed on the auxiliary component 700 such that the center of the opening 703 of the auxiliary component 700 is aligned with the central axis of the gel 101 disposed on the auxiliary component 700 within a predetermined error range. Furthermore, when mounting the gel 101 onto the objective lens using the auxiliary component 700, the auxiliary component 700, the objective lens, and the gel 101 can be positioned such that the optical axis of the objective lens is aligned with the central axis of the gel 101 within a predetermined error range. Figure 8 (b) shows the optical axis 820 of the objective lens.

[0131] Next, a sheet-like auxiliary component 900 is shown as an example. Figure 9 This is a diagram illustrating a sheet-like auxiliary component 900 in an exemplary embodiment. Figure 9 (a) shows a cross-section of the auxiliary component 900. Additionally, in Figure 9 Figure (b) shows the upper surface of the auxiliary component 900. The auxiliary component 900 is an annular sheet component comprising an annular sheet. The auxiliary component 900 is made of a rigid material such as metal, resin, or ceramic. The auxiliary component 900 has a first surface 901 on which the gel 101 is disposed, a second surface 902 facing the frame component of the objective lens, and an opening 903.

[0132] In addition, Figure 9 The illustrated implementation example demonstrates the use of Figure 1In the case of gel 101 in shape (b), the auxiliary component 900 has an opening diameter 903 smaller than the outer diameter of the gel (e.g., the diameter of the bottom surface is 9 mm), for example, 7 mm. Additionally, the opening diameter 903 of the auxiliary component 900 is larger than the effective diameter of the objective lens. A first surface 901 of the auxiliary component 900 faces the gel 101, and a portion of the first surface 901 is fixed relative to the gel 101 by an adhesive force. The first surface 901 includes the opening 903. Furthermore, the outer diameter of the first surface 901 is larger than the outer diameter of the gel (e.g., the diameter of the bottom surface is 9 mm), for example, 14 mm. A second surface 902 of the auxiliary component 900, disposed on the objective lens side, has an adhesive force that adheres tightly to the front end side of the objective lens. This adhesive force allows the auxiliary component 900 to be mounted on and removed from the objective lens.

[0133] In addition, Figure 9 In this example, the auxiliary component 900 has a protruding portion 910. The shape of the inner side of the protrusion 910 can be formed to match the shape of the front end of the objective lens. For example, in Figure 9 In (a), as described later. Figure 10 As shown, the protrusion 910 is formed at a 60-degree angle to match the shape of the front end of the objective lens. In addition, the outer diameter of part A of the auxiliary component 900 is made to match the diameter of the front end of the objective lens, for example, it is formed to be 12.1 mm.

[0134] Figure 10 This diagram illustrates the installation of the auxiliary component 900 towards the objective lens. Figure 10 (a) shows the front end portion 1000 of the objective lens. The front end portion 1000 includes a front lens 1001, a lens support frame 1002, and a front outer frame 1003. The outer diameter of the upper surface of the front outer frame 1003 is 12 mm. In addition, the tilt angle of the outer surface of the front outer frame 1003 is 60 degrees.

[0135] Figure 10 (b) is a diagram illustrating the mounting of the auxiliary component 900 to the front end portion 1000 of the objective lens. The protrusion 910 of the auxiliary component 900 is configured to fit into the front end frame 1003 of the objective lens, thereby enabling positioning with the gel 101 in close contact with the front end lens 1001 of the objective lens.

[0136] In another embodiment, the gel 101 can be disposed on the auxiliary member 900 such that the center of the opening 903 of the auxiliary member 900 is aligned with the central axis of the gel 101 disposed on the auxiliary member 900 within a predetermined error range. Furthermore, when mounting the gel 101 onto the objective lens using the auxiliary member 900, the protrusion 910 of the auxiliary member 900, the objective lens, and the gel 101 can be positioned such that the optical axis of the objective lens is aligned with the central axis of the gel 101 within a predetermined error range. Figure 10 (b) shows the optical axis 1020 of the objective lens.

[0137] Next, an example of using an elastomer in an auxiliary component will be explained. Figure 11 This is a diagram showing an example of an auxiliary component 1100 using an elastomer. Figure 11 (a) shows a cross-section of the auxiliary component 1100. Additionally, in Figure 11 Figure (b) shows the upper surface of the auxiliary component 1100. The auxiliary component 1100 is made of an elastomer material, such as polyurethane rubber. The auxiliary component 1100 includes a first surface 1101 on which the gel 101 is disposed, a second surface 1102 facing the frame component of the objective lens, and an opening 1103. Figure 11 In one example, the auxiliary component 1100 has a cover structure having an opening 1103 formed of a thin rubber.

[0138] In addition, Figure 11 The illustrated implementation example demonstrates the use of Figure 1 In the case of gel 101 of shape (a), the auxiliary component 1100 has an opening diameter 1103 smaller than the outer diameter of the gel (e.g., the diameter of the bottom surface 10 mm), for example, 7 mm. Furthermore, the opening diameter 1103 of the auxiliary component 1100 is larger than the effective diameter of the objective lens. The first surface 1101 includes the opening 1103. Additionally, the outer diameter of the first surface 1101 is larger than the outer diameter of the gel (e.g., the diameter of the bottom surface 10 mm), for example, 16 mm.

[0139] Additionally, the auxiliary component 1100 includes, for example, a fixing part ( Figure 11 (Part B) The fixing part has a structure that applies a spring force to the objective lens for fixing the auxiliary component 1100. The inner diameter A of the fixing part can be smaller than the outer diameter of the side surface of the objective lens, for example, in order to apply a spring force for fixing the auxiliary component 1100. For example, if the outer diameter of the side surface of the objective lens is 16 mm, the inner diameter A of the fixing part can be 15.8 mm.

[0140] Furthermore, the auxiliary component 1100 includes a flange 1111 for easy clamping. Therefore, by stretching a portion of the flange 1111, the user can utilize the elasticity of the rubber to expand the inner diameter of the auxiliary component 1100, temporarily enlarging the fixing portion having an inner diameter smaller than the outer diameter of the objective lens's side surface and introducing it to the objective lens's side surface. And, when the flange 1111 is released, the auxiliary component 1100 can be mounted and fixed to the objective lens using the elastic force recovering from elastic deformation.

[0141] Figure 12 This is a diagram illustrating the mounting of the auxiliary component 1100 to the objective lens in an exemplary embodiment. Figure 12(a) shows the front end portion 1200 of the objective lens. The front end portion 1200 includes a front lens 1201, a lens support frame 1202, and a front outer frame 1203. The outer diameter of the front outer frame 1203 is, for example, 16 mm.

[0142] Figure 12 (b) is a diagram illustrating the mounting of the auxiliary component 1100 to the front end portion 1200 of the objective lens. The fixing portion of the auxiliary component 1100 ( Figure 12 The portion B is expanded by stretching flange 1111, thereby being pulled in such a way that the front end frame 1203 of the objective lens is inserted into it. Then, by releasing it, the auxiliary component 1100 is spring-loaded and fixed to the front end portion 1200 of the objective lens. For example, the fixing part ( Figure 12 The inner diameter of the region of part B is smaller than the outer diameter of the front outer frame 1203, so the auxiliary component 1100 is fixed to the front part 1200 of the objective lens by elasticity.

[0143] In addition, such as Figure 12 As shown, when the front end frame 1203 is pulled in such a way that it is inserted into the auxiliary component 1100, the front end lens 1201 is positioned in close contact with the gel 101. In addition, since the inner diameter of the fixing part is slightly smaller than the outer diameter of the front end frame 1203, the auxiliary component 1100 can be fixedly mounted to the front end portion 1200 by elasticity.

[0144] Additionally, the length of the fixed part to which the elastic force is applied ( Figure 12 The length of part B along the side of the objective lens is preferably 5 mm or more. For example, if it is less than 5 mm, it is sometimes difficult to apply elastic force, and the auxiliary part 1100 is prone to displacement. Additionally, for example, by extending the auxiliary part along the side of the objective lens... Figure 12 The protrusion 1211 shown in (c) (e.g., a 0.2 mm protrusion) is provided as a fixing part at a position closer to the gel side than part B, so that even if the length of part B is less than 5 mm, the auxiliary part 1100 is difficult to shift.

[0145] In another embodiment, the gel 101 can be positioned on the auxiliary component 1100 such that the center of the opening 1103 of the auxiliary component 1100 is aligned with the central axis of the gel 101 disposed on the auxiliary component 1100 within a specified error range. Furthermore, when mounting the gel 101 onto the objective lens using the auxiliary component 1100, the auxiliary component 1100, the objective lens, and the gel 101 can be positioned such that the optical axis of the objective lens is aligned with the central axis of the gel 101 within a specified error range. Figure 12 (b) shows the optical axis 1220 of the objective lens.

[0146] Next, the installation method of installing gel 101 on auxiliary component 1100 will be described. Figure 13 This diagram illustrates an example of a method for mounting gel 101 onto auxiliary component 1100. The mounting of gel 101 onto auxiliary component 1100 is performed, for example, using a mold 1301 for gel 101. The mold 1301 for gel 101 can be made of a material with good release properties, such as ABS (acrylonitrile, butadiene, styrene) resin, polyethylene, polypropylene, or fluoropolymer. In another example, a silicone or fluorinated release agent may be applied to the surface of the mold 1301 for example.

[0147] exist Figure 13 In the example, gel 101 is installed on auxiliary component 1100, for example, according to the following steps.

[0148] Step 1: Insert tool 1302 into auxiliary component 1100. Additionally, tool 1302 may have the same outer diameter as the front end portion 1200 of the objective lens.

[0149] Step 2: Inject the pre-gelling liquid into the mold 1301 of gel 101.

[0150] Step 3: Place the auxiliary component 1100 and the tool 1302 into the mold 1301 of the gel 101. The position of the auxiliary component 1100 can be marked on the mold 1301 of the gel 101, for example. Then, the liquid before gelation is cured under gel curing conditions to form the gel 101.

[0151] Step 4: Take and lift the auxiliary component 1100 and tool 1302 to remove the gel 101 from the mold 1301.

[0152] Step 5: Stretch the flange 1111 of the auxiliary component 1100 to enlarge it, and remove the auxiliary component 1100 and gel 101 from the tool 1302.

[0153] Furthermore, in step 2, for example, by placing the auxiliary component 1100 on the mold 1301 of the gel 101 in a matching manner with a mark on the mold 1301 of the gel 101 indicating the position of the auxiliary component 1100, the gel 101 and the auxiliary component 1100 can be aligned.

[0154] Furthermore, in step 4, for example, the auxiliary component 1100 is configured such that the adhesion force between it and the gel 101 is higher than the adhesion force between the mold 1301 and the gel 101, thus enabling the gel 101 to be tightly adhered to the auxiliary component 1100 and pulled out of the mold 1301. Figure 13 In step 5, gel 101 is shown being mounted on gel unit 1300 of auxiliary component 1100.

[0155] Figure 14 This diagram illustrates another example of a mounting method for attaching gel 101 to auxiliary component 1100. Figure 14 The text shows the relationship with... Figure 13 The corresponding process. However, in Figure 14 In the example, the mold 1401 of gel 101 has a protrusion 1411 formed to match the shape of auxiliary component 1100 and tool 1302. By fitting auxiliary component 1100 and tool 1302 into the protrusion 1411 of mold 1401, the position of auxiliary component 1100 can be installed in accordance with gel 101.

[0156] Next, a sheet-like auxiliary component 1500 is shown. Figure 15 This is a diagram illustrating a sheet-like auxiliary component 1500 in an exemplary embodiment. Figure 15 A cross-section of the auxiliary component 1500 is shown in (a). Additionally, in Figure 15 Figure (b) shows the upper surface of the auxiliary component 1500.

[0157] The sheet-like auxiliary component 1500 includes, for example, a ring-shaped and sheet-like annular sheet component. The auxiliary component 1500 is made of materials such as metal, resin, rubber, or adhesive sheet. The auxiliary component 1500 includes a first surface 1501 on which gel is disposed, a second surface 1502 facing the frame component of the objective lens, and an opening 1503. Furthermore, the second surface 1502 has a sealing force, and a protective component 1510 (e.g., a covering) is attached to cover the second surface 1502 to protect it.

[0158] In addition, Figure 15 The illustrated implementation example demonstrates the use of Figure 1 In the case of gel 101 of shape (a), the auxiliary component 1500 has an opening diameter 1503 smaller than the outer diameter of the gel (e.g., the diameter of the bottom surface 10 mm), for example, 7 mm. The opening diameter 1503 of the auxiliary component 1500 is larger than the effective diameter of the objective lens. The first surface 1501 of the auxiliary component 1500 faces the gel 101, and a portion of the first surface 1501 is fixed relative to the gel 101 by an adhesive force. The first surface 1501 includes the opening 1503. In addition, the outer diameter of the first surface 1501 is larger than the outer diameter of the gel (e.g., the diameter of the bottom surface 10 mm), for example, 12 mm. The auxiliary component 1500 has, for example, a structure that fits tightly against the front end portion of the objective lens, allowing it to be mounted on the front end portion of the objective lens and also to be removed.

[0159] Figure 16 This is a diagram illustrating the installation of the auxiliary component 1500 toward the objective lens in an exemplary embodiment. Figure 16(a) indicates the shape of the front end portion 1600 of the objective lens. The front end portion 1600 includes a front lens 1601, a lens support frame 1602, and a front outer frame 1603. The outer diameter of the surface 1605 of the front frame of the front outer frame 1603 is, for example, 12 mm.

[0160] Figure 16 (b) is a diagram illustrating the installation of the auxiliary component 1500 onto the front end portion 1600 of the objective lens. For example, the second surface 1502 of the auxiliary component 1500, disposed on the objective lens side, has a tight contact with the surface 1605 of the objective lens front end frame. Furthermore, the auxiliary component 1500 can be installed onto the objective lens by the tight contact and can be removed. Additionally, the opening diameter of the opening 1503 of the auxiliary component 1500 is formed, for example, the same as the diameter of the front end surface of the objective lens, thereby allowing the auxiliary component 1500 to be easily installed at a position relatively defined relative to the optical axis of the objective lens. As a result, when the gel 101 is installed onto the objective lens using the auxiliary component 1500, the auxiliary component 1500, the objective lens, and the gel 101 are positioned such that the optical axis of the objective lens and the central axis of the gel 101 are aligned within a specified error range. Figure 16 (b) shows the optical axis 1620 of the objective lens.

[0161] Figure 17 This diagram illustrates an installation method for attaching gel 101 to auxiliary component 1500. The components of the mold 1700 for gel 101 can be made of materials with good release properties, such as ABS (acrylonitrile, butadiene, styrene) resin, polyethylene, polypropylene, or fluoropolymer. In another example, a silicone or fluorinated release agent may be applied to the surface of the mold component of the mold 1700 for gel 101.

[0162] exist Figure 17 In the example, gel 101 is installed on auxiliary component 1500, for example, according to the following steps.

[0163] Step 1: Inject the pre-gelling liquid into the gel mold 1700, place the auxiliary component 1500 in the gel mold 1700, and solidify the pre-gelling liquid under gel solidification conditions to form gel 101. The position of the auxiliary component 1500 can be, for example, marked on the mold 1700 of gel 101.

[0164] Step 2: Holding the auxiliary component 1500, lift the gel 101 and remove it from the gel mold 1700. Lay the protective component 1510 on the adhesive surface of the auxiliary component 1500 and store it. By laying the protective component 1510 on the auxiliary component 1500, the adhesive force of the auxiliary component 1500 can be protected.

[0165] In addition, in order to apply a tight gripping force to the auxiliary component 1500, double-sided tape or the like can be pasted on the second side 1502 of the auxiliary component 1500, or the second side 1502 can also be formed of adhesive rubber.

[0166] Figure 18 This diagram illustrates another example of a method for mounting gel 101 onto auxiliary component 1500. Figure 18 In the example, gel 101 is installed on auxiliary component 1500, for example, according to the following steps.

[0167] Step 1: Inject the pre-gelling liquid into the mold 1800 of gel 101, and solidify the pre-gelling liquid under gel solidification conditions to form gel 101.

[0168] Step 2: Place the auxiliary component 1500 on the tool 1801.

[0169] Step 3: After the gel has solidified, the gel mold 1800 is reversed. Additionally, the tool 1801 may have a protrusion 1802 with the same outer diameter as the front end portion 1600 of the objective lens. Thus, by engaging the opening 1503 of the auxiliary component 1500 with the protrusion 1802, the position of the auxiliary component 1500 can be matched with the outer diameter of the tool 1801.

[0170] Step 4: Place the auxiliary component 1500 and the tool 1801 into the mold 1800 of the gel 101. The mold 1800 may have a protrusion 1803. Thus, by fitting the tool 1801 into the protrusion 1803 of the gel mold 1800, the position of the auxiliary component 1500 can be aligned with the gel 101, and the gel 101 can be mounted onto the auxiliary component 1500.

[0171] Step 5: Remove tool 1801 from mold 1800 of gel 101.

[0172] Step 6: Remove mold 1800. Install protective component 1510 on auxiliary component 1500. By installing protective component 1510 on auxiliary component 1500, the clamping force of auxiliary component 1500 can be protected.

[0173] If the adhesion of the second surface 1502 of the auxiliary component 1500 is too strong, it may be impossible to remove the tool 1801 from the mold 1800 of the gel 101 in step 5. Therefore, increasing the adhesion, for example, when applying double-sided tape, can be done after removing the mold 1800 in step 6. Alternatively, the protective component 1510 of the auxiliary component 1500 can be made annular, with a shape approximately the same as the second surface of the auxiliary component 1500. The protective component 1510 can be installed on the auxiliary component 1500 before the auxiliary component 1500 is placed on the tool 1801 in step 2 to advance the process. In this case, the application of the protective component 1510 in step 6 is eliminated.

[0174] In addition, if the adhesion of the second side 1502 of the auxiliary component 1500 is weak, the auxiliary component 1500 may easily fall off the tool 1801, making the operation difficult. Therefore, in order to facilitate the adhesion and removal operation, in one example, an electrically peelable adhesive tape can be used.

[0175] The electrically peelable adhesive tape is, for example, a tape with adhesive strength, and also has the property that the adhesive strength can be reduced by applying voltage, thereby making peeling easier. In one example, an electrically peelable adhesive tape manufactured by VIGteQnos can be used. For example, in step 2, the electrically peelable adhesive tape is disposed between the tool 1801 and the auxiliary component 1500, with the auxiliary component 1500 disposed on the tool 1801. Thus, the auxiliary component 1500 can be adhered to the tool 1801 using the electrically peelable adhesive tape, and the auxiliary component 1500 can be stably held on the tool 1801.

[0176] In addition, when removing tool 1801 from mold 1800 of gel 101 in step 5, the adhesive force is reduced by applying voltage to the electro-peelable adhesive tape, thereby making it easy to peel auxiliary component 1500 from the electro-peelable adhesive tape and making it easy to remove tool 1801.

[0177] Next, an auxiliary component 1900 consisting of multiple parts is shown. Figure 19 This is a diagram illustrating an auxiliary component 1900 composed of multiple components according to an embodiment. Figure 19 In the example, the auxiliary component 1900 includes a first component 1901 and a second component 1902.

[0178] The first component 1901 is formed of materials such as metal, resin, rubber, or adhesive sheet. The first component 1901 may be a ring-shaped sheet having an opening 1903 with a thickness of 0.2 mm, an outer diameter of Φ12 mm, and an inner diameter of Φ7 mm. In one example, the first component 1901 may have features similar to those of the auxiliary component 1500. The first component 1901 has a first surface for close contact with the gel 101.

[0179] The second component 1902 is formed, for example, of an elastomer such as polyurethane rubber. The second component 1902 is a surface (e.g., the back side) facing away from the first surface (opposite direction), and has a second surface disposed on the objective lens side. Furthermore, a portion of the second component extends along the optical axis of the objective lens, for example, as a frame component covering the objective lens. The second component 1902 has, for example, an opening 1903 with an inner diameter of Φ7mm. In one example, the second component 1902 may have features similar to those of the auxiliary component 1100.

[0180] At least one of the third surface of the first component 1901 (the surface facing (opposite direction) to the first surface (e.g., the back side)) and the fourth surface of the second component 1902 (the surface facing (opposite direction) to the second surface (e.g., the back side)) may be tightly fitted together, with the first component 1901 and the second component 1902 fitting together using the third and fourth surfaces. The first component 1901 is, for example, a component that is harder than the second component 1902.

[0181] Additionally, the second component 1902 includes a fixing part (part B in the figure) which has a structure for applying an elastic force to fix it to the objective lens. The inner diameter A of the fixing part can, for example, be smaller than the outer diameter of the side surface of the objective lens. For example, if the outer diameter of the side surface of the objective lens is 16 mm, the inner diameter A of the fixing part can be 15.8 mm.

[0182] Furthermore, the second component 1902 includes a flange 1911 for easy clamping. Therefore, by stretching the flange 1911, the user can utilize the elasticity of the rubber to enlarge the inner diameter of the second component 1902, temporarily expanding the fixing portion, which has an inner diameter smaller than the outer diameter of the objective lens's side surface, and introducing it to the side surface of the objective lens. Afterwards, by releasing the flange, the second component 1902 can be mounted and secured to the objective lens using elasticity.

[0183] Figure 20 This is a diagram illustrating the mounting of the auxiliary component 1900 toward the objective lens in an exemplary embodiment. Figure 20 (a) shows the front end portion 2000 of the objective lens. The front end portion 2000 includes a front lens 2001, a lens support frame 2002, and a front outer frame 2003. The outer diameter of the surface 2005 of the front frame of the front outer frame 2003 is, for example, 12 mm.

[0184] Figure 20 (b) illustrates the mounting of the auxiliary component 1900 to the front end portion 2000 of the objective lens. Figure 20 As shown, by bonding the first component 1901 to the second component 1902, the gel 101 can be disposed on the objective lens when the second component 1902 is mounted on the objective lens.

[0185] Additionally, the installation of the gel 101 onto the auxiliary component 1900 in the embodiment is, for example, in... Figure 13 or Figure 14 The steps described can be performed similarly by using auxiliary component 1900 instead of auxiliary component 1100. Alternatively, in another embodiment, in Figure 17 In the steps described, gel 101 is attached to first component 1901 instead of auxiliary component 1500. Subsequently, auxiliary component 1900 can also be fabricated by tightly attaching first component 1901 to second component 1902.

[0186] Next, the mounting position of the auxiliary component and the gel 101 will be explained. Regarding the mounting position of the gel 101 on the auxiliary component, it is preferable, for example, that the outer periphery of the surface of the gel 101 that is in close contact with the auxiliary component is positioned outside the opening of the auxiliary component. If the outer periphery of the gel 101 is at the opening, the adhesion of that portion is weaker, and the gel 101 can easily detach from the auxiliary component. For example, when the objective lens moves due to objective lens switching or changes in the observation position, if a portion of the outer periphery of the bottom surface of the gel 101 is not in close contact with the auxiliary component, it can sometimes easily peel off. Furthermore, in this case, it can sometimes easily peel off, especially if the side opposite to the direction of movement is not in close contact. Therefore, in one embodiment, the gel 101 is positioned on the auxiliary component such that the outer periphery of the surface of the gel 101 that is in close contact with the auxiliary component is positioned outside the opening of the auxiliary component.

[0187] Figure 21 This is a diagram illustrating the configuration of gel 101 towards the auxiliary component. Figure 21 In the diagram, shaded area 2100 represents the area where gel 101 and auxiliary components are in close contact with each other. For example, in... Figure 21 In (a), a portion of the outer periphery of the bottom surface of gel 101 enters the inner side of the opening of the auxiliary component and is not in close contact with the auxiliary component. In this case, for example, due to objective lens movement, the resistance to applied forces weakens, and gel 101 may sometimes peel off from the auxiliary component. On the other hand, in Figure 21 In (b), the outer periphery of the surface of gel 101 that is in close contact with the auxiliary component is disposed outside the opening of the auxiliary component. Therefore, it has high stability against forces applied due to objective lens movement, etc. In addition, the offset between the center of the surface of gel 101 that is in close contact with the auxiliary component and the center of the auxiliary component is preferably within a specified allowable range (e.g., within 1 mm).

[0188] Furthermore, the shape of gel 101 can be varied. For example, such as Figure 21 (c) and Figure 21As illustrated in (d), the bottom surface of gel 101 can also be polygonal. In such cases, it is also preferable to mount gel 101 to the auxiliary component with the bottom surface of gel 101 positioned outside the opening of the auxiliary component. For example, in Figure 21 In (c), a portion of the outer periphery of the bottom surface of gel 101 enters the inner side of the opening, resulting in a weaker adhesion. On the other hand, in Figure 21 In (d), for example, the outer periphery of the surface of the gel 101 that is in close contact with the auxiliary component is disposed outside the opening of the auxiliary component. Therefore, it has high stability against forces applied due to objective lens movement, etc. Furthermore, the same applies when the shape of the gel 101 or the shape of the opening of the auxiliary component becomes complex or different.

[0189] In addition, in the above embodiments, the installation of the auxiliary components onto the gel 101 can also be controlled by a computer, for example.

[0190] Figure 22 This is a diagram of an installation system 2200 for installing an execution auxiliary component onto a gel 101, illustrating an embodiment. Figure 22 The mounting system 2200 of (a) includes, for example, a computer 2201, a stage controller 2202, a support controller 2203, a camera 2204, and a mounting device 2205. The mounting device 2205 is, for example, an actuator.

[0191] Computer 2201 is, for example, an information processing device with computing capabilities, such as a server computer, a personal computer (PC), a mobile PC, or a tablet terminal.

[0192] The mounting device 2205 includes, for example, a stage 2211 for placing and moving a mounting component 2210, on which gel 101 is disposed, in the X and Y directions, and an electric support 2212 for moving an auxiliary component 2230 in the Z direction. The electric support 2212 includes a holder 2213 for holding the auxiliary component 2230. In one example, the mounting component 2210 may be a mold for making gel 101.

[0193] Regarding the platform 2211, for example, it is moved in the X direction under the control of the platform controller 2202. Additionally, regarding the platform 2211, for example, it is moved in the Y direction under the control of the platform controller 2202. The electric support 2212 moves the cage 2213 in the Z direction, for example, under the control of the support controller 2203.

[0194] Computer 2201, for example, moves the mounting component 2210, which is equipped with gel 101 and is supported on mounting stage 2211, in the X and Y directions by sending control signals to stage controller 2202. Additionally, computer 2201 moves the retainer 2213 in the Z direction by sending control signals to support controller 2203.

[0195] For example, computer 2201 performs the following control: determines the moving position of mounting device 2205 such that the center of the opening of auxiliary component 2230 comes to the central axis of gel 101 disposed on mounting component 2210, and causes mounting device 2205 to press auxiliary component 2230 onto gel 101 at the determined position.

[0196] Therefore, the gel 101 can be mounted on the auxiliary component 2230 using the mounting system 2200. First, for example, the mounting component 2210 with the gel 101 is fixed on the stage 2211. Furthermore, a predetermined peripheral position from the edge of the mounting component 2210 with the gel 101 to the gel 101 is measured in advance. Additionally, the auxiliary component 2230 is mounted on the holder 2213 of the electric support 2212.

[0197] Computer 2201 sends control signals, for example, to stage controller 2202 and support controller 2203, using stage 2211 to move the configuration component 2210, on which gel 101 is disposed, to a predetermined position for docking with auxiliary component 2230. However, some positional offsets may occur when the configuration component 2210 is placed on stage 2211 and when the auxiliary component 2230 is mounted on the retainer 2213 of the electric support 2212. As a result, the configuration may sometimes result in insufficient positional accuracy for docking gel 101 with auxiliary component 2230.

[0198] Therefore, the computer 2201 sends a control signal to the support controller 2203 to lower the position of the auxiliary component 2230 in the Z direction to the limit position that does not contact the configuration component 2210.

[0199] Computer 2201 confirms, for example, the X-axis and Y-axis positions (X1, Y1) of the designated edge of the configuration component 2210 of gel 101, and the X-axis and Y-axis positions (X2, Y2) of the designated portion of the outer side of auxiliary component 2230, by means of images captured by imaging device 2204.

[0200] Then, computer 2201 calculates X = X1 ~ X2, Y = Y1 ~ Y2, and uses stage 2211 to move the configuration component 2210 to a position where the difference (X0, Y0) between the predetermined edge position of the configuration component 2210 and the outer position of the auxiliary component 2230 does not match: X0 ~ X, Y0 ~ Y. Finally, computer 2201 sends a control signal to motorized support 2212 to lower the position of auxiliary component 2230 in the Z direction until it contacts gel 101. Afterward, computer 2201 sends a control signal to motorized support 2212 to raise the position of auxiliary component 2230 in the Z direction. Thus, gel 101 can be placed in close contact with auxiliary component 2230 at the appropriate position and removed from configuration component 2210.

[0201] Alternatively, when using the installation system 2200 to install the gel 101 onto the auxiliary component 2230, a mold 2250 having multiple gels 101 formed can also be used. Figure 22 (b) is an illustration of a mold 2250 having multiple gels 101 formed. Figure 22 In (b), a plurality of gels 101 are formed at equal intervals on the mold 2250. In this case, the positions of the predetermined edges of the mold 2250 and the peripheral positions of the individual gels 101 contained in the mold 2250 are also measured and determined in advance. As a result, the mounting system 2200 can be used to make the gels 101 fit tightly against the auxiliary component 2230 in the appropriate position.

[0202] In addition, in the above embodiments, for example, the lens support frame 602, the main body 803, the front outer frame 1003, the front outer frame 1203, the front outer frame 1603, and the front outer frame 2003 are examples of frame components of the objective lens.

[0203] Furthermore, in the above-described embodiment, the auxiliary component on which the gel 101 is mounted may be referred to as a gel unit, for example. The gel unit is used, for example, to mount the gel 101 on the objective lens of a light signal detection device. The gel unit is configured, for example, to fill the space between the objective lens and the holding member. For example, with the gel unit mounted on the frame member of the objective lens, the second surface of the auxiliary component faces the frame member of the objective lens, and a portion of the first surface of the auxiliary component is fixed relative to the gel 101 at a position outside the effective diameter of the objective lens, with the gel 101 in close contact with the objective lens.

[0204] Furthermore, as illustrated in the embodiments described above, for example, when the gel unit is mounted on the frame member, a portion of the auxiliary member extends along the optical axis of the objective lens in a manner that covers the frame member of the objective lens. For example, a portion of the auxiliary member extends in a circular shape in a direction opposite to the gel 101.

[0205] Additionally, as illustrated in the embodiments described above, the auxiliary component may be shaped to have the same shape as at least a portion of the frame component (e.g., its outer periphery). Furthermore, the auxiliary component, for example, if it is an elastomer, may also have a shape slightly smaller than the outer periphery of the frame component.

[0206] Furthermore, as illustrated in the embodiments described above, in one example, the auxiliary component may have a structure that mechanically mounts it relative to the frame component, thereby fixing the auxiliary component to the frame component. Examples of such structures include threaded grooves, hooks, and face-fitting members. For instance, the annularly extending portion of the auxiliary component may have a mechanically mounted structure such as threaded grooves, hooks, and face-fitting members.

[0207] Furthermore, as illustrated in the embodiments described above, in one example, the portion of the second surface facing the first surface may have a clamping force. This portion of the second surface facing the first surface is, for example, a portion of the surface facing away from the first surface, such as the back side of the first surface. For example, the auxiliary member is fixed to the frame member by the clamping force of this portion. Additionally, the annularly extending portion of the auxiliary member may, for example, have elasticity.

[0208] The above embodiments are illustrative, but the embodiments are not limited thereto. For example, the steps described above are illustrative, and the embodiments are not limited thereto. Where possible, the order of the steps can be changed, additional steps can be included, or some steps can be omitted. For example, in the above-described installation of gel 101 onto the auxiliary component, gel 101 can be cured before the auxiliary component comes into contact with gel 101, or gel 101 can be cured after the auxiliary component comes into contact with gel 101.

[0209] Figure 23 This is a diagram illustrating the hardware structure of computer 2201 in an exemplary implementation. It is used for implementing... Figure 23 The hardware structure of the computer 2201 includes, for example, a processor 2301, a memory 2302, a storage device 2303, a reading device 2304, a communication interface 2306, and an input / output interface 2307. Furthermore, the processor 2301, memory 2302, storage device 2303, reading device 2304, communication interface 2306, and input / output interface 2307 are interconnected, for example, via a bus 2308.

[0210] Processor 2301 may be a single processor, a multi-processor, or a multi-core processor. Processor 2301 provides some or all of the functions of the computer 2201 by executing a program described with the steps described above using memory 2302. For example, processor 2301 controls stage controller 2202 and support controller 2203 by reading and executing a program stored in storage device 2303.

[0211] The memory 2302 is, for example, a semiconductor memory, which may include RAM and ROM regions. The storage device 2303 is, for example, a hard disk, flash memory, or other semiconductor memory or external storage device. Note that RAM is an abbreviation for Random Access Memory. ROM is an abbreviation for Read Only Memory.

[0212] The reading device 2304 accesses the removable storage medium 2305 according to the instructions of the processor 2301. The removable storage medium 2305 may be implemented as, for example, a semiconductor device, a medium for inputting and outputting information via magnetic action, or a medium for inputting and outputting information via optical action. Furthermore, the semiconductor device may be, for example, a USB (Universal Serial Bus) memory. Additionally, the medium for inputting and outputting information via magnetic action may be, for example, a magnetic disk. The medium for inputting and outputting information via optical action may be, for example, a CD-ROM, DVD, Blu-ray Disc, etc. (Blu-ray is a registered trademark). CD is short for CompactDisc. DVD is short for Digital Versatile Disk.

[0213] Communication interface 2306 communicates with another device according to instructions from processor 2301. For example, communication interface 2306 sends control signals to stage controller 2202 and support controller 2203 as instructed by processor 2301.

[0214] The input / output interface 2307 can be, for example, an interface between an input device and an output device. Input devices include, for example, devices that receive instructions from a user, such as a keyboard, mouse, or touch panel. Output devices include, for example, display devices such as a monitor, and sound devices such as a speaker.

[0215] Figure 24 This is a diagram illustrating the structure of a microscope apparatus 1 according to one embodiment. Figure 24 The microscope apparatus 1 shown is an inverted microscope used for observing the sample S from below, and is an example of a light signal detection device for detecting light signals. Figure 24As shown, the microscope apparatus 1 includes a light source 2, an objective lens 3 mounted on a converter 4, and a camera 5. The objective lens 3 is a so-called immersion objective lens used with the space between the objective lens 3 and the container filled with immersion liquid. Alternatively, the objective lens 3 may be an objective lens specially designed for attaching gels. The container C for holding the sample S is, for example, a glass base bag, and is an example of a holding component for holding the sample S. The sample S is not particularly limited and may be, for example, a biological sample such as cells. The sample S may also be a three-dimensional cultured spheroid, organoid, or other sample with a thickness of several hundred μm.

[0216] In addition, Figure 24 The diagram shows an example of microscope apparatus 1 as an inverted microscope, but microscope apparatus 1 can also be an upright microscope. In this case, gel 101 can also fill the space between the glass cover and the objective lens 3, which is another example of a sample holding component.

[0217] The microscope apparatus 1 illuminates the sample S with light from the light source 2, and the camera 5 detects the light from the sample S, thereby obtaining an image of the sample S. The observation method of the microscope apparatus 1 is not particularly limited. For example, an image of the sample S can be obtained through bright-field observation or fluorescence observation. Other observation methods are also possible, such as phase difference observation or differential interference observation.

[0218] The microscope apparatus 1 includes a gel 101 with a higher refractive index than air and an auxiliary component 11 for preparing the gel 101 between the liquid-immersed objective lens 3 and the container C, in place of the immersion liquid.

[0219] Unlike immersion liquid, gel 101 is non-flowing. Therefore, compared to immersion liquid, which is held between objective lens 3 and container C by surface tension, gel 101 can be easily positioned in the observation optical path between objective lens 3 and container C using auxiliary component 11, and removal from the observation optical path is equally easy. Therefore, even when switching between immersion and dry objectives, the switching operation can be performed quickly. Furthermore, the non-flowing gel 101 does not flow down from objective lens 3 during observation like immersion liquid. Therefore, it does not contaminate objective lens 3 and its surroundings, making cleaning after use easy. Additionally, since there is no extreme volume reduction due to evaporation, it does not require additional supply during observation like immersion liquid, making it easy to handle long-term observations. Moreover, gel 101 has relatively high adhesion, so it can remain stably between objective lens 3 and container C regardless of whether the observation is oblique or lateral. Thus, gel 101 is much easier to handle than immersion liquid. In addition, by using the auxiliary component 11 to process the gel 101 into a gel unit, the burden on the user associated with the use of the liquid immersion objective lens can be greatly reduced.

[0220] Furthermore, gel 101, like the immersion liquid, has a higher refractive index than air. Therefore, the performance of the liquid immersion objective 3 can be utilized to achieve a high numerical aperture, enabling the acquisition of bright images at high resolution. Additionally, compared to the case where air is present, the refractive index difference between the objective lens and the container C and the sample S is also smaller. Therefore, similar to the case using the immersion liquid, spherical aberrations caused by the refractive index difference between the objective lens and the container C and the sample S can be suppressed, and the sample S can be observed at greater depths. Therefore, by using gel 101, the three-dimensional structure of the sample can be observed well.

[0221] As described above, the microscope apparatus 1, which uses gel 101 and auxiliary components 11 for observation, does not impose an excessive burden on the user even when using the liquid immersion objective lens 3, and allows for good observation of the sample S. Furthermore, it allows for smooth switching between dry and liquid immersion objectives, thus minimizing observation interruptions associated with objective lens switching and enabling efficient observation in a short period.

[0222] Furthermore, by using the auxiliary component 11 to process the gel 101 and placing the gel 101 on the microscope apparatus 1, the operation becomes easier, and the gel 101 can be easily mounted onto the objective lens. As a result, for example, compared to methods that involve direct contact with the gel using tweezers or the like, gel damage during processing can be suppressed.

[0223] So far, the bottom surface of Gel 101 has been taken as a flat surface, but various advantages can be achieved by designing the shape of the bottom surface.

[0224] The following examples illustrate other shapes of gel 101. Figure 25 An example of an embodiment is a gel 2501 with a convex surface 2510 (the center of the bottom surface of the gel 101) having a bottom surface 2520 (the outer edge region of the gel 101). Figure 25 In gel 2501 of (a), with Figure 1 Compared to gel 101 in (a), a convex surface 2510 with a radius of curvature of 6 mm is added to the bottom surface 2520. Additionally, in Figure 25 In (b), an auxiliary component 2502, on which gel 2501 is mounted, is installed on the front end portion of the objective lens. Furthermore, the front end portion of the objective lens includes a front lens 2511 and a lens support frame 2512.

[0225] When an objective lens has a concave surface at a low light level, a concave surface with a small radius of curvature can be configured without reducing the overall positive optical power of the lens. This allows for correction of image plane curvature by adjusting the petzmann curve from near zero. The front end of the objective lens has a lower light level compared to other parts of the objective lens, making it easier to configure a concave surface with a small radius of curvature. However, conventional liquid immersion objectives suffer from problems such as liquid entering the concave surface and being unable to be cleaned, making it impossible to configure a concave surface at the front end. But if gel 101 is shaped like gel 2501, the front end of the objective lens can be configured as a concave shape.

[0226] The bottom surface 2520 of gel 2501 is in close contact with the auxiliary component 2502. Because the radius of curvature of the convex surface 2510 of the bottom surface 2520 of gel 2501 is small, the part of the bottom surface 2520 of gel 2501 that is in close contact with the auxiliary component 2502 is deformed to match the shape of the auxiliary component 2502.

[0227] Figure 26 An example of an embodiment is a gel 2601 with a concave surface 2630 (the center of the bottom surface of gel 101) having a bottom surface 2620 (the outer edge region of gel 101). Figure 26 In gel 2601 of (a), with Figure 1 Compared to gel 101 in (a), a stepped concave surface 2630 is added to the bottom surface 2620 of gel 2601, and the bottom surface 2620 of gel 2601 is shaped as a ring-shaped column with an outer diameter of Φ10mm, a width of 1.5mm, and a height of 0.2mm. Furthermore, in Figure 26 In (b), an auxiliary component 2602, on which gel 2601 is mounted, is installed on the front end portion of the objective lens. Furthermore, the front end portion of the objective lens includes a front lens 2611 and a lens support frame 2612.

[0228] According to gel 2601, such as Figure 26 As shown in (b), the stepped concave surface 2630 of the gel 2601 can be embedded into the convex shape of the front lens 2611 of the objective lens, making it difficult for the gel 2601 to shift when the holding component (sample) moves laterally (e.g., in the X and Y directions). However, for example, if the height of the step is too high, it becomes difficult to install and remove the gel 2601. Therefore, for example, it is preferable that the height of the step is within 1 mm and the bottom surface of the gel 2601 is approximately flat.

[0229] Figure 27 An example of an embodiment is a gel 2701 with a spherical concave surface, the bottom surface 2720 (corresponding to the center and outer edge regions of the bottom surface of gel 101). Figure 27 (a) of gel 2701 and Figure 1 Compared to gel 101 in (a), the bottom surface 2720 is spherical (radius of curvature 70 mm). Additionally, in Figure 27In (b), an auxiliary component 2702, on which gel 2701 is mounted, is installed on the front end portion of the objective lens. Furthermore, the front end portion of the objective lens includes a front lens 2711 and a lens support frame 2712.

[0230] The convex surface of the objective lens tip has a large radius of curvature. If the radius of curvature becomes smaller, it becomes difficult to correct the bending of the objective lens surface. Due to the large radius of curvature, at the point where the auxiliary component 2702 and the gel 2701 are in contact, the gel 2701 deforms on the plane and adheres tightly to the auxiliary component 2702. Therefore, the adhesion force between the gel 2701 and the auxiliary component 2702 becomes high, making it difficult for the gel 2701 to separate from the holding component when the objective lens is changed or when the holding component (sample) moves laterally (e.g., in the X and Y directions).

[0231] As above Figures 25 to 27 As illustrated, the gel can be of various shapes. For example, the bottom surface of the gel can be flat, concave, convex, spherical, stepped, etc. In addition, when the bottom surface of the gel includes some concave shapes, it is preferably generally flat (the height difference is within 1 mm).

[0232] The above describes several implementation methods. However, these implementation methods are not limited to those described above and should be understood to include various modifications and alternatives to the above implementation methods. For example, it should be understood that various implementation methods can be embodied by modifying the constituent elements without departing from their spirit and scope. Furthermore, it should be understood that various implementation methods can be implemented by appropriately combining the multiple constituent elements disclosed in the above implementation methods. Moreover, those skilled in the art should understand that various implementation methods can be implemented by deleting several constituent elements from all the constituent elements shown in the implementation methods, or by adding several constituent elements to the constituent elements shown in the implementation methods.

Claims

1. An optical signal detection device, characterized in that, have: Objective lens; as well as The gel unit comprises a gel and auxiliary components. The auxiliary component has a first surface, a second surface, and an opening. The diameter of the opening is smaller than the outer diameter of the gel, and larger than the effective diameter of the objective lens. The first surface faces the gel, and a portion of the first surface is fixed relative to the bottom surface of the gel by an adhesive force. The center of the bottom surface does not contact the auxiliary component. The auxiliary component can be attached to and detached relative to the objective lens while the bottom surface of the gel is fixed to a portion of the first surface of the auxiliary component by a tight adhesion force. With the gel unit mounted on the frame of the objective lens, the second surface faces the frame of the objective lens, and a portion of the first surface is fixed relative to the gel at a position outside the effective diameter of the objective lens, with the gel in close contact with the objective lens. The gel is formed by solidifying the pre-gelling liquid in a state where the auxiliary component is in contact with the pre-gelling liquid of the gel.

2. The optical signal detection device according to claim 1, characterized in that, With the gel unit mounted on the frame component, a portion of the auxiliary component extends along the optical axis of the objective lens in such a way that it covers the frame component of the objective lens.

3. The optical signal detection device according to claim 2, characterized in that, The auxiliary component has elasticity. The auxiliary component is fixed to the frame component by the force of elastic recovery from elastic deformation.

4. The optical signal detection device according to claim 1, characterized in that, The second surface of the auxiliary component is shaped to have the same shape as the outer periphery of the frame component.

5. The optical signal detection device according to claim 2, characterized in that, The auxiliary component has a structure that allows it to be mechanically mounted to the frame component. The auxiliary component is fixed to the frame component by the aforementioned structure.

6. The optical signal detection device according to claim 2, characterized in that, The portion of the second surface that faces the first surface has a contact force. The auxiliary component is fixed to the frame component by the clamping force.

7. The optical signal detection device according to claim 1, characterized in that, The auxiliary component is sheet-shaped. The second surface has a surface facing opposite to the first surface. The second surface is fixed relative to the frame component by a pressing force.

8. The optical signal detection device according to claim 1, characterized in that, The outer periphery of the surface of the gel that is in close contact with the auxiliary component is positioned further outward than the opening.

9. The optical signal detection device according to claim 1, characterized in that, The auxiliary components include a first component and a second component. The first component has the first surface. The second component has the second surface. The first component is sheet-like. A portion of the second component extends along the optical axis of the objective lens in a manner that covers the frame component of the objective lens. The first component is harder than the second component.

10. The optical signal detection device according to claim 1, characterized in that, The offset between the center of the surface of the gel that is in close contact with the auxiliary component and the center of the auxiliary component is within 1 mm.

11. The optical signal detection device according to any one of claims 1 to 10, characterized in that, The optical signal detection device also includes a holding component that holds the sample between the objective lens and the sample. The gel unit fills the space between the objective lens and the holding component.

12. The optical signal detection device according to claim 1, characterized in that, A portion of the auxiliary component extends in a ring shape in the direction opposite to the gel.

13. The optical signal detection device according to claim 12, characterized in that, The annular extension of the auxiliary component is elastic.

14. The optical signal detection device according to claim 12, characterized in that, The annular extension of the auxiliary component has a mechanically designed mounting structure.

15. The optical signal detection device according to claim 12, characterized in that, The second surface, which faces the first surface, has a pressing force.

16. The optical signal detection device according to claim 1, characterized in that, The auxiliary components include a first component and a second component. The first component has the first surface. The first component is sheet-like. A portion of the second component extends in a ring shape in a direction opposite to the gel. The first component is harder than the second component.

17. A method for manufacturing a gel unit, wherein, The gel unit is used to mount on the objective lens of the optical signal detection device. The gel unit comprises a gel and auxiliary components. The auxiliary component has a first surface and an opening. The diameter of the opening is smaller than the outer diameter of the gel. The first surface faces the gel. A portion of the first surface is fixed relative to the bottom surface of the gel by an adhesive force. The center of the bottom surface does not contact the auxiliary component. The manufacturing method is characterized by including the following steps: The first step involves injecting a pre-gelling liquid into a gel mold used to solidify the gel. The second step is to place the auxiliary component into the gel mold; The third step involves solidifying the pre-gelling liquid to form a gel. as well as The fourth step involves removing the gel and the auxiliary components from the gel mold. The process is performed in the order of the first process, the second process, the third process, and the fourth process.

18. A method for manufacturing a gel unit, wherein, The gel unit is used to mount on the objective lens of the optical signal detection device. The gel unit comprises a gel and auxiliary components. The auxiliary component has a first surface and an opening. The diameter of the opening is smaller than the outer diameter of the gel. The first surface faces the gel. A portion of the first surface is fixed relative to the bottom surface of the gel by an adhesive force. The center of the bottom surface does not contact the auxiliary component. The manufacturing method is characterized by including the following steps: The first step is to solidify the pre-gelling liquid, which has been injected into a gel mold containing the auxiliary components, to form a gel. as well as The second step involves removing the gel and the auxiliary components from the gel mold. The process is performed in the order of the first process and the second process.

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