Method for detecting the contribution of soil microorganisms with nitrifying properties to the cytokinins made available to the leaves of corn by intra-radical action

By establishing mathematical models and detection methods, the contribution rate of soil microorganisms to maize leaf cytokinin was detected, solving the problem of detecting the role of soil microorganisms in the roots and improving crop nitrogen use efficiency and grain yield.

CN116779049BActive Publication Date: 2026-07-24HENAN UNIV OF SCI & TECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
HENAN UNIV OF SCI & TECH
Filing Date
2023-05-24
Publication Date
2026-07-24

AI Technical Summary

Technical Problem

Existing technologies lack methods to detect the increase in maize leaf cytokinin content caused by soil microorganisms with nitrifying properties through root action, which affects crop nitrogen use efficiency and food production.

Method used

A mathematical model was established to detect the contribution rate of soil microorganisms to maize leaf cytokinins using Equations 1 and 2, including the treatment and detection of the experimental and control groups. The CTK content in maize leaves was measured using enzyme-linked immunosorbent assay (ELISA). Combined with soil nitrification inhibitor and nitrate nitrogen treatment, EB and EN values ​​were calculated to determine the contributing pathways.

Benefits of technology

This study enabled the accurate detection of CTK content in maize leaves by soil microorganisms through root action, revealing the pathways by which they regulate crop growth and promote crop growth and increased grain yield.

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Abstract

The present application relates to a method for detecting the contribution of soil microorganisms with nitrification characteristics to the cytokinin of maize leaves through intraradical action, and belongs to the field of agricultural biology. The present application detects the cytokinin content of maize leaves under the action of excess nitrate nitrogen when nitrification is inhibited, and the CTK content of maize leaves when inoculated with soil microorganism strains with nitrification characteristics, and establishes a mathematical model, so as to realize the detection of the contribution of soil microorganisms to the cytokinin of maize leaves through intraradical and extraradical action. The present application has good application prospects in the detection of the action pathway of soil microorganisms with nitrification characteristics in regulating crop growth.
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