A gene encoding alkane monooxygenase in living acinetobacter, a protein encoding the gene and its application

By being separated from Live Bacterium KJ-1 and a single-oxygenase encoding gene, and realizes its efficient expression in E. coli, it solves the gap in the research gap in the repair of diesel pollution. Efficient oil degradation enzymes have important industrial application value.

CN116790526BActive Publication Date: 2025-01-28BIOTECH CENT OF SHANDONG ACAD OF SCI
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Patent Information

Application Number
CN202310407695.4
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2023-04-17
Publication Date
2025-01-28
Estimated Expiration
2043-04-17

AI Technical Summary

Technical Problem

When using active Acinetobacterium for repair of diesel-contaminated sites, the prior art lacks molecular research on its petroleum hydrocarbon degradation mechanism and related functions, which limits its effective utilization as an efficient biological resource.

Method used

By being separated from Live Bacterium KJ-1 and cloned alkane single-oxygenase encoding genes, the restructuring expression carrier PSUMO1-Alkb1_1 is constructed, and its efficient expression is achieved in E. coli, and the reorganized gene engineering bacteria are performed Essence

Benefits of technology

The in vitro expression and reorganization expression of alkaine plus oxygenase in the livelobe is realized, and it provides a kind of petroleum degradation enzyme that has high enzymes in the acidic environment and has important industrial application value.

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Abstract

The present invention relates to the fields of genetic engineering and enzyme engineering, and in particular to an alkane monooxygenase encoding gene, an encoding protein and its application in living Acinetobacter. The alkane monooxygenase encoding gene is derived from living Acinetobacter, and the deposit number is CGMCC No.20664; the nucleotide sequence of the alkane monooxygenase encoding gene is shown in SEQ ID NO.1. The present invention expresses the gene sequence of alkane-1-monooxygenase from living Acinetobacter KJ-1 in vitro for the first time to help study the function and regulatory mechanism of the protein, which is of great significance for the future study of bioremediation mechanisms of pollutant sites such as petroleum hydrocarbons; the present invention provides a petroleum hydrocarbon degradation enzyme alkane-1-monooxygenase, which has good acid resistance, a wide temperature suitable range, can be efficiently expressed in Escherichia coli, and has important industrial application value.
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Citation Information

Patent Citations

  • Acinetobacter as well as microbial inoculum and application thereof

    CN112725240A

  • Monooxygenase gene of alkane degradation bacterial and its coded protein

    CN1900284A