A device for testing the fertility of a plant population

Abstract

The present application relates to the field of hotbed, specifically relates to a kind of plant population fertility test integrated device, it includes box, incubator, industrial robot and controller, the inside of box is provided with light source and image collector;Incubator is placed in the inside of box, incubator includes culture dish and liquid storage structure, culture dish is placed in the inside of liquid storage structure, and permeation structure is formed in the bottom of culture dish, which can be passed by liquid but not by solid, the bottom of culture dish is connected with electronic tag, industrial robot includes mechanical paw and mechanical arm, and the reader for identifying electronic tag is connected on mechanical paw.Image collector is used to monitor seed, and the reader and electronic tag ensure that culture dish taken from different boxes can accurately return to the original position, in the process of taking and placing culture dish, culture solution in the inside of culture dish is backflowed to the inside of liquid storage structure through permeation structure, avoid culture solution in culture dish to be spilled or contaminated in the process of taking and placing.

Description

Technical Field

[0001] This invention relates to the field of promoting breeding beds, and more specifically to an integrated device for testing the reproductive capacity of plant populations. Background Technology

[0002] Low fertility, low seed vigor, and difficulty in germination are generally considered important reasons for obstacles in the life history of endangered species (Xie Zongqiang and Li Qingmei, 2000). Seed size and germination are two key characteristics in plant life history and important processes for plant adaptation to the environment and continuation of life (Zhang Yingjuan et al., 2011), playing a crucial role in population renewal and resistance to adverse environments (Wei Chunqiang et al., 2012). Seed size represents the investment the parent plant makes in its offspring, and together with germination, it affects seedling survival, individual fitness, and competition, thus influencing ecological processes such as population renewal (Gutterman, 2000; Donohu ​​et al., 2005; Wang Juhong et al., 2007). Seed vigor and germination rate directly affect population renewal and survival, and even determine the rarity and distribution range of some plants (Quero et al., 2008). Among them, seed germination rate represents the germination ability of seeds, and average germination time measures the speed of seed germination; both are important indicators for detecting seed quality (Zhou Anpei et al., 2012). Low seed yield, poor quality, low viability and germination rate, and a low proportion of seeds converting to seedlings are common phenomena in endangered plants (Zhang Yingjuan et al., 2008). It is unclear whether the seeds of *Pinus pentaphyllum* from the Dabie Mountains also exhibit these characteristics.

[0003] Currently, the problems that trouble people include: how do fundamental issues affecting seed germination, such as the effect of the seed coat on seed imbibition and germination? Under artificial cultivation conditions, are storage methods, storage time, and seed source related to seed vigor and germination? What kind of germination substrate is more suitable?

[0004] To obtain answers to the above questions, people need to conduct experiments measured in years and months. The more types of seeds there are, the more personnel are needed for the experiments, and the longer it takes. This poses a significant challenge to the funding and reliability of the experiments. If the number of experimental personnel can be reduced, the funding required for the experiments can be significantly reduced, and the number of human errors can also be reduced. Therefore, a device that can replace or assist humans in conducting such experiments is needed. Summary of the Invention

[0005] The purpose of this invention is to provide an integrated device for testing the reproductive capacity of plant populations, so as to solve the technical problem of the lack of devices for testing the reproductive capacity of plant populations.

[0006] To solve the above-mentioned technical problems, the present invention specifically provides the following technical solution:

[0007] An integrated device for testing the reproductive capacity of plant populations includes: a box made of heat-insulating material, with a light source and an image acquisition device installed inside the box; a culture vessel placed inside the box and located within the illumination range of the light source and the acquisition range of the image acquisition device, the culture vessel including a petri dish and a liquid storage structure, the petri dish for placing seeds, the liquid storage structure for storing liquid, the bottom of the petri dish being immersed in the liquid stored inside the liquid storage structure, the bottom of the petri dish having a permeable structure that allows liquid to pass through but not solids, and an electronic tag connected to the bottom of the petri dish; and an industrial robot including a mechanical gripper capable of grasping the petri dish and a mechanical arm for driving the mechanical gripper to move, the mechanical gripper being connected to a reader for identifying the electronic tag.

[0008] Furthermore, the mechanical claw and the mechanical arm are connected by a tension / compression sensor, which is used to measure the weight change when the mechanical claw grasps and suspends the culture dish to obtain the water absorption of the seeds.

[0009] Furthermore, the top of the culture dish is formed with a flange that extends radially outward along its edge.

[0010] Furthermore, the portion of the bottom of the culture dish where the permeable structure is not formed has an upwardly protruding protrusion.

[0011] Furthermore, the bottom of the culture dish has an insertion port, a sealing ring is fitted onto the insertion port, and the top of the liquid storage structure has a receiving port. The insertion port and the receiving port are connected and sealed by the sealing ring.

[0012] Furthermore, the liquid storage structure is a horizontally arranged tubular structure, and the socket is vertically arranged at the top of the liquid storage structure.

[0013] Furthermore, the liquid storage structure is arranged in a shape that has the maximum heating area on a horizontal plane.

[0014] Furthermore, it also includes a constant temperature water circulation machine, the liquid storage structure being connected to the constant temperature water circulation machine, the constant temperature water circulation machine being used to circulate a liquid at a constant temperature inside the liquid storage structure to provide moisture to the seeds and keep the temperature inside the box constant.

[0015] Furthermore, the housing includes a drawer slidably connected to its body, and a first drive that drives the drawer to slide. The actuator of the first drive and the incubator are both fixedly connected to the drawer, and the drawer has a vertical wall higher than the incubator.

[0016] Furthermore, the constant temperature water circulator includes a quick-connect connector connecting its working end to the liquid storage structure, and a second driver that drives the quick-connect connector to or separate from the liquid storage structure. The quick-connect connector passes through the housing and is slidably connected to the housing. The quick-connect connector is fixedly connected to the actuator of the second driver. A drain pump or drain valve is connected to the lowest point of the liquid storage structure. Before the second driver drives the quick-connect connector to separate from the housing, the constant temperature water circulator stops working, and the liquid inside the liquid storage structure is drained through the drain pump or drain valve.

[0017] Compared with the prior art, this application has the following advantages:

[0018] 1. In the germination experiment, there is no need for manual observation of whether the filter paper is moldy or whether the seed germination has ended. This task is completed by a machine vision inspection system consisting of an image acquisition unit and a controller, which saves manpower.

[0019] 2. During the process of weighing and measuring the size of seeds, an industrial robot is used to transfer the petri dishes. Readers and electronic tags ensure that the petri dishes taken out from different boxes can be accurately returned to their original positions, avoiding errors in handling.

[0020] 3. During the process of the industrial robot picking up and placing the petri dish, the culture medium inside the petri dish flows back to the storage structure through the permeation structure, which prevents the culture medium in the petri dish from being spilled or contaminated during the picking and placing process. Attached Figure Description

[0021] To more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are merely exemplary, and those skilled in the art can derive other embodiments based on the provided drawings without creative effort.

[0022] Figure 1 This is a schematic diagram of the structure of the box and incubator;

[0023] Figure 2 Top view of a petri dish being placed on an industrial robot;

[0024] Figure 3 for Figure 2 A sectional view along the AA direction and its enlarged partial view;

[0025] Figure 4 A 3D view and a magnified view of a portion of the petri dish being removed by an industrial robot;

[0026] Figure 5 A three-dimensional view of the petri dish;

[0027] Figure 6 This is a three-dimensional schematic diagram of the incubator;

[0028] Figure 7 A three-dimensional view of a system consisting of multiple boxes;

[0029] Figure 8 A front view of a system consisting of multiple enclosures;

[0030] Figure 9 for Figure 8 A cross-sectional view along the BB direction;

[0031] Figure 10 for Figure 8 A cross-sectional view along the CC direction;

[0032] Figure 11 This is a schematic diagram of the working structure of a constant temperature water circulation machine;

[0033] The labels in the diagram represent the following:

[0034] 1-Box housing; 1a-Light source; 1b-Image acquisition unit; 1c-Drawer; 1c1-Vertical wall; 1d-First drive;

[0035] 2-Culturer; 2a-Cultured dish; 2a1-Permeation structure; 2a2-Electronic tag; 2a3-Flange; 2a4-Protrusion; 2a5-Socket; 2a6-Sealing ring; 2b-Liquid storage structure; 2b1-Socket; 2b2-Steel plate;

[0036] 3-Industrial robot; 3a-Mechanical gripper; 3a1-Gripper; 3b-Reader; 3c-Tension / compression sensor;

[0037] 4-Constant temperature water circulation machine; 4a-Quick connector; 4b-Second drive. Detailed Implementation

[0038] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0039] In order to obtain plant seeds for the experiment, the following preparations need to be made:

[0040] For several consecutive years, researchers harvested cones from natural and cultivated populations. Then, they randomly selected 8-10 cones from each plant in both populations, totaling 60 cones from each population. After naturally air-drying, the seeds were separated. Using cones as units, the seeds that sank were selected by water selection, which were considered plump seeds. The plumpness rate and sterility rate of the seeds were then statistically analyzed.

[0041] The seeds from each plant were mixed, and 100 seeds with normal appearance were randomly selected. This was repeated 6 times, and the weight of 100 seeds was measured using an electronic balance accurate to 0.0001g. The length and width of the seed scales and the length, width, and thickness of the normal-looking seeds were measured using vernier calipers accurate to 0.01mm.

[0042] To assess the reproductive capacity of a plant population, it is also necessary to test seed vigor, imbibition capacity, and germination ability. The testing steps are as follows:

[0043] The same batch of seeds was air-dried naturally for 2 months, and then stored under different conditions:

[0044] Storage condition 1: Store in a nylon mesh bag at room temperature.

[0045] Storage condition 2: Store in a sealed bag at low temperature (4℃).

[0046] Storage condition 3: Store for 0, 6, 10 or 12 months.

[0047] The vigor of Dabie Mountain five-needle pine seeds was determined using the red tetrazolium (TTC) staining method, in accordance with the ISTA (International Seed Testing Association) seed testing procedures (ISTA, 1996).

[0048] Before conducting the germination experiment, the seeds were disinfected with 0.1% potassium permanganate for 30 minutes, rinsed with clean water and then rinsed with distilled water. They were then placed in a 90mm petri dish with culture medium and placed in an intelligent light incubator with a light intensity of 1000lx. Germination experiments were conducted under different imbibition and germination conditions.

[0049] Imbispiration condition 1: Cut off the seed coat at the end of the radicle (retain the inner seed coat).

[0050] Imbispiration condition 2: Retain the seed coat.

[0051] Germination condition 1: 10 / 14h light / dark ratio, 20 / 10℃ temperature ratio.

[0052] Germination condition 2: 10 / 14h light / dark ratio, 25 / 15℃ temperature ratio.

[0053] Germination condition 3: 10 / 14h light / dark ratio, 30 / 20℃ temperature ratio.

[0054] Germination condition 4: 10 / 14h light / dark ratio, 35 / 25℃ temperature ratio.

[0055] Germination condition 5: Use sterilized filter paper as the germination substrate, preferably double-layered filter paper.

[0056] Germination condition 6: Use sterilized gravel (passed through a 0.8mm sieve) as the germination substrate. The gravel should be such that the seeds are just fixed in the sand and will not move when water is added.

[0057] During the experiment, before the seeds fully absorb water, the seeds need to be taken out every 24 hours to dry the surface moisture and weighed. Water should be added or the filter paper should be replaced as needed. After the water absorption amount has not changed significantly for 3 consecutive days, the water absorption experiment is stopped and the water absorption amount is calculated. Water absorption amount = (total seed weight - seed weight before water absorption) / seed weight before water absorption (Baskinetal., 2004).

[0058] In the germination experiment, distilled water needs to be changed daily, and filter paper should be replaced if necessary to prevent mold growth. For ungerminated seeds, the seed coat is removed to check for empty seeds to determine their viability (Narbonae et al., 2013). The final germination rate is the percentage of the total number of germinated seeds out of the number of plump seeds measured. Germination is defined as the radicle breaking through the seed coat. Statistics are collected every 24 hours, and germinated seeds are removed. The germination rate and average germination time are used as indicators. At the end of germination, no change in the number of germinated seeds for 5 consecutive days is used as the marker for the end of germination.

[0059] In summary, the experimental process is complex and involves a large amount of repetitive physical labor, which significantly consumes the working time of the experimental personnel and reduces their work efficiency. To address these issues, this application provides:

[0060] like Figure 1-5 As shown, this application provides:

[0061] An integrated device for testing the reproductive capacity of plant populations includes a box 1, a culture vessel 2, an industrial robot 3, and a controller.

[0062] The enclosure 1 is made of insulation material, and the enclosure 1 can maintain a basically constant temperature by circulating hot or cold air.

[0063] The chamber 1 is equipped with a light source 1a and an image acquisition device 1b. The culture vessel 2 is placed inside the chamber 1 and is located in the illumination area of ​​the light source 1a and the acquisition area of ​​the image acquisition device 1b. The light source 1a is used to provide 14 / 10h of light to the culture vessel 2. The image acquisition device 1b is used to identify the contents of the culture vessel 2. For example, when the seeds inside the culture vessel 2 germinate or when the filter paper used as the culture medium becomes moldy, they can be identified and judged by machine vision. Even if the images acquired by the image acquisition device 1b are identified manually, the efficiency of manual identification can be greatly improved.

[0064] The culture vessel 2 includes a culture dish 2a and a liquid storage structure 2b. The culture dish 2a is placed inside the liquid storage structure 2b. The bottom of the culture dish 2a has a permeable structure 2a1 that allows liquid to pass through but not solids. The culture dish 2a and the liquid storage structure 2b are connected through the permeable structure 2a1. In this embodiment, the permeable structure 2a1 consists of several tiny pores, and it can also be a liquid-permeable material such as paper or cloth. When the liquid storage structure 2b is filled with culture medium, the culture medium enters the culture dish 2a through the permeable structure 2a1. When the culture dish 2a is separated from the liquid storage structure 2b, the culture medium inside the culture dish 2a flows back into the liquid storage structure 2b through the permeable structure 2a1.

[0065] The industrial robot 3 includes a mechanical gripper 3a capable of grasping a petri dish 2a and a robotic arm that drives the mechanical gripper 3a to move. The robotic arm is not shown in the figure. Any robotic arm with three degrees of freedom can be used, preferably with three degrees of freedom of linear movement in the XYZ directions. The robotic arm can be any model capable of grasping the petri dish 2a. To facilitate the operation of the industrial robot 3, a workbench can be placed next to the industrial robot 3. The industrial robot 3 can place the petri dish 2a on the workbench and wait for the test personnel to process it.

[0066] An electronic tag 2a2 is attached to the bottom of the petri dish 2a, and a reader 3b for identifying the electronic tag 2a2 is attached to the mechanical gripper 3a. The function of the reader 3b is to identify the electronic tag 2a2 through the principle of RFID, and then send the identification information of the petri dish 2a to the controller. This eliminates the need for manual registration, improves work efficiency, and reduces the possibility of errors in manual data entry.

[0067] Image acquisition device 1b is connected to the controller and performs machine vision inspection on petri dish 2a through the controller. Reader 3b is connected to the controller and transmits the recognition result of electronic tag 2a2 to the controller.

[0068] The controller typically includes an industrial computer and a human-machine interface terminal. The human-machine interface terminal generally includes a monitor, keyboard and mouse, and a handheld reader. The handheld reader is used for manual identification of the electronic tag 2a2 and input of relevant information.

[0069] In summary, the conveniences this device provides to test personnel include:

[0070] In the germination experiment, there is no need for manual observation of whether the filter paper is moldy or whether the seed germination has ended. This task is completed by a machine vision inspection system consisting of an image acquisition unit 1b and a controller, saving manpower. During the process of weighing and measuring the size of the seeds, the industrial robot 3 transfers the culture dish 2a. The reader 3b and the electronic tag 2a2 ensure that the culture dish 2a taken from different boxes 1 can be accurately returned to its original position, avoiding errors in picking and placing. During the process of the industrial robot 3 picking and placing the culture dish 2a, the culture solution inside the culture dish 2a flows back to the liquid storage structure 2b through the permeation structure 2a1, preventing the culture solution in the culture dish 2a from being spilled or contaminated during the picking and placing process.

[0071] Further:

[0072] The mechanical gripper 3a and the robotic arm are connected via a tension / compression sensor 3c. The tension / compression sensor 3c communicates with the controller and transmits the measurement results of the tension / compression sensor 3c to the controller.

[0073] The function of the tension and compression sensor 3c is to detect the weight of the mechanical claw 3a, and also to detect the total weight of the mechanical claw 3a and the culture dish 2a it grasps. In the imbibition test, the liquid in the liquid storage structure 2b is first removed by some method, such as pumping it away or discharging it through a valve connected to the liquid storage structure 2b. After the liquid in the culture dish 2a is allowed to drain naturally through the permeation structure 2a1, the mechanical claw 3a and the culture dish 2a are weighed as a whole. The weight difference between the two weighings is the weight of the water absorbed by the seed.

[0074] Further:

[0075] The top of the petri dish 2a has a flange 2a3 that extends radially outward along its edge.

[0076] The function of flange 2a3 is to facilitate the gripping of culture dish 2a by mechanical claw 3a. Each claw of mechanical claw 3a is connected to a lever arm with a bayonet 3a1. The lever arm is retracted under the action of the three-jaw cylinder and clamps flange 2a3 through bayonet 3a1, so that mechanical claw 3a can lift, lower and move culture dish 2a horizontally by mechanical arm.

[0077] Further:

[0078] The bottom of the petri dish 2a, where the permeable structure 2a1 has not yet formed, has an upward protrusion 2a4.

[0079] The purpose of the protrusion 2a4 is to facilitate the more complete drainage of liquid inside the petri dish 2a. The protrusion 2a4 is located in the center of the petri dish 2a, and the electronic tag 2a2 is attached to the back of the protrusion 2a4, which can prevent the reader 3b from reading the electronic tag 2a2 on the adjacent petri dish 2a.

[0080] Further:

[0081] The bottom of the petri dish 2a has a socket 2a5, and a sealing ring 2a6 is fitted onto the socket 2a5. The top of the liquid storage structure 2b has a socket 2b1, and the socket 2a5 and the socket 2b1 are connected by a socket.

[0082] The petri dish 2a and the liquid storage structure 2b are basically sealed together, which can reduce the difficulty of liquid evaporation and prevent steam from affecting the image acquisition of the image acquisition device 1b.

[0083] Further:

[0084] The liquid storage structure 2b is a horizontally arranged tubular structure, and the socket 2b1 is vertically arranged on the top of the liquid storage structure 2b.

[0085] The liquid storage structure 2b allows for rapid introduction or discharge of liquid, facilitating the replacement of distilled water inside the petri dish 2a by staff and ensuring the purity of the distilled water inside the petri dish 2a.

[0086] This also facilitates cleaning of the inside of the liquid storage structure 2b. The industrial robot 3 can pull the petri dish 2a out of the liquid storage structure 2b, insert the sealing cap (not shown in the figure) into the socket 2b1, and then circulate liquid inside the liquid storage structure 2b to clean it.

[0087] Further:

[0088] The liquid storage structure 2b is arranged in a shape that has the largest heating area on the horizontal plane.

[0089] For example, the S-shaped or vortex-shaped structures commonly used in underfloor heating are preferably made of copper. In this way, as long as the liquid circulating in the liquid storage structure 2b has a constant temperature, the inside of the box 1 will also have a constant temperature. The outside of the liquid storage structure 2b is covered with a steel plate 2b2, and the liquid storage structure 2b is sandwiched between the upper and lower parts of the steel plate 2b2 to protect it.

[0090] Further:

[0091] It also includes a constant temperature water circulation machine 4, and a liquid storage structure 2b is connected to the constant temperature water circulation machine 4.

[0092] The constant temperature water circulator 4 is used to circulate distilled water at a constant temperature inside the liquid storage structure 2b, so that the temperature inside the box 1 is constant. The constant temperature water circulator 4 includes a cooling water circulator and a hot water circulator, which are connected to both ends of the liquid storage structure 2b through a three-way valve. When the required temperature inside the box 1 is higher than the room temperature, the hot water circulator works, and when the required temperature inside the box 1 is lower than the room temperature, the cooling water circulator works.

[0093] Further:

[0094] The housing 1 includes a drawer 1c that is slidably connected to its body, and a first driver 1d that drives the drawer 1c to slide. The actuator of the first driver 1d and the incubator 2 are both fixedly connected to the drawer 1c. The drawer 1c has a vertical wall 1c1 that is higher than the incubator 2.

[0095] The first actuator 1d is a cylinder, and the drawer 1c is slidably connected to the body of the box 1 via several rollers. The first actuator 1d drives the drawer 1c to slide along the rollers, so that the culture vessel 2 is moved out of the box 1, thereby facilitating the industrial robot 3 to move the culture dish 2a into or out of the liquid storage structure 2b.

[0096] Further:

[0097] The constant temperature water circulation machine 4 includes a quick connector 4a that connects its working end to the liquid storage structure 2b, and a second driver 4b that drives the quick connector 4a to be inserted into or separated from the liquid storage structure 2b. The quick connector 4a passes through the housing 1 and is slidably connected to the housing 1. The quick connector 4a is fixedly connected to the actuator of the second driver 4b.

[0098] The second actuator 4b is a cylinder. To prevent the connection between the liquid storage structure 2b and the constant temperature water circulation machine 4 from being disconnected due to the operation of the first actuator 1d, the liquid inside the liquid storage structure 2b is emptied using a pump or valve before the first actuator 1d is operated. Then the second actuator 4b drives the quick connector 4a to separate from the liquid storage structure 2b. Then the first actuator 1d drives the drawer 1c to move, thereby pushing the incubator 2 out of the box 1.

[0099] The above embodiments are merely exemplary embodiments of this application and are not intended to limit this application. The scope of protection of this application is defined by the claims. Those skilled in the art can make various modifications or equivalent substitutions to this application within its substance and scope of protection, and such modifications or equivalent substitutions should also be considered to fall within the scope of protection of this application.

Claims

1. An integrated device for testing the reproductive capacity of plant populations, characterized in that, include, The box (1) is made of thermal insulation material, and a light source (1a) and an image acquisition device (1b) are installed inside the box (1). The culture vessel (2) is placed inside the box (1) and located in the irradiation range of the light source (1a) and the acquisition range of the image acquisition device (1b). The culture vessel (2) includes a culture dish (2a) and a liquid storage structure (2b). The culture dish (2a) is used to place seeds, and the liquid storage structure (2b) is used to store liquid. The bottom of the culture dish (2a) is immersed in the liquid stored inside the liquid storage structure (2b). The bottom of the culture dish (2a) has a permeable structure (2a1) that allows liquid to pass through but not solids to pass through. An electronic tag (2a2) is connected to the bottom of the culture dish (2a). An insertion port (2a5) is formed at the bottom of the culture dish (2a), and a receiving port (2b1) is formed at the top of the liquid storage structure (2b). The insertion port (2a5) and the receiving port (2b1) are connected by insertion and insertion. An industrial robot (3) includes a mechanical gripper (3a) capable of grasping the petri dish (2a) and a mechanical arm that drives the mechanical gripper (3a) to move. A reader (3b) for identifying the electronic tag (2a2) is connected to the mechanical gripper (3a). The liquid storage structure (2b) is a horizontally arranged tubular structure, and a drain pump or drain valve is connected to the lowest point of the liquid storage structure (2b); The mechanical claw (3a) and the mechanical arm are connected by a tension and compression sensor (3c), which is used to measure the weight change when the mechanical claw (3a) grasps and suspends the petri dish (2a) to obtain the water absorption of the seeds.

2. The integrated device for testing plant population reproductive capacity according to claim 1, characterized in that, The top of the culture dish (2a) has a flange (2a3) that extends radially outward along its edge.

3. The integrated device for testing plant population reproductive capacity according to claim 1, characterized in that, The bottom of the culture dish (2a) where the permeation structure (2a1) is not formed has an upward protrusion (2a4).

4. The integrated device for testing plant population reproductive capacity according to claim 1, characterized in that, A sealing ring (2a6) is fitted onto the socket (2a5), and the socket (2a5) and the receptacle (2b1) are sealed by the sealing ring (2a6).

5. The integrated device for testing plant population reproductive capacity according to claim 4, characterized in that, The liquid storage structure (2b) is arranged in a shape that has the largest heating area on a horizontal plane.

6. The integrated device for testing plant population reproductive capacity according to claim 5, characterized in that, It also includes a constant temperature water circulation machine (4), the liquid storage structure (2b) is connected to the constant temperature water circulation machine (4), the constant temperature water circulation machine (4) is used to circulate a liquid at a constant temperature inside the liquid storage structure (2b) to provide moisture to the seeds and keep the temperature inside the box (1) constant.

7. The integrated device for testing plant population reproductive capacity according to claim 6, characterized in that, The box (1) includes a drawer (1c) slidably connected to its body, and a first driver (1d) for driving the drawer (1c) to slide. The actuator of the first driver (1d) and the incubator (2) are both fixedly connected to the drawer (1c). The drawer (1c) has a vertical wall (1c1) higher than the incubator (2).

8. The integrated device for testing plant population reproductive capacity according to claim 7, characterized in that, The constant temperature water circulation machine (4) includes a quick connector (4a) connecting its working end to the liquid storage structure (2b), and a second driver (4b) driving the quick connector (4a) to be inserted into or separated from the liquid storage structure (2b). The quick connector (4a) passes through the housing (1) and is slidably connected to the housing (1). The quick connector (4a) is fixedly connected to the actuator of the second driver (4b). Before the second driver (4b) drives the quick connector (4a) to separate from the housing (1), the constant temperature water circulator (4) stops working, and the liquid inside the liquid storage structure (2b) is drained through the drain pump or drain valve.

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