通过可逆反应定量活细胞内谷胱甘肽的核磁共振方法
By utilizing the reversible reaction between a fluorine probe and GSH, atomic-resolution quantitative detection and dynamic monitoring of GSH in living cells were achieved using 19F-NMR, solving the detection challenges in existing technologies and providing a rapid and accurate detection method.
CN116818822BActive Publication Date: 2026-07-17NANKAI UNIV
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- NANKAI UNIV
- Filing Date
- 2023-05-25
- Publication Date
- 2026-07-17
AI Technical Summary
Technical Problem
Existing irreversible probe methods are difficult to achieve real-time, atomic-resolution detection of glutathione (GSH) levels in living cells, and fluorescence methods are difficult to avoid GSH consumption and background signal interference.
Method used
Fluorine probes are used to generate adducts with GSH through a reversible reaction. Intracellular GSH content is detected by 19F-NMR. Fluorine probes have selectivity and specificity, enabling quantitative detection and dynamic monitoring at the atomic resolution level.
Benefits of technology
This method enables rapid and accurate quantitative detection and dynamic monitoring of intracellular GSH. The probe exhibits low toxicity to cells, good stability and reproducibility, and avoids background signal interference.
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Abstract
本发明公开一种通过可逆反应定量活细胞内谷胱甘肽的核磁共振方法，具体公开一种氟探针，氟探针以吡啶环作为母环，迈克尔受体作为反应官能团并将F或CF3引入反应官能团邻位进行19F‑NMR采集；氟探针能够与谷胱甘肽(GSH)发生快速可逆反应并生成共价连接产物，并在19F‑NMR谱图中明显观测到自由标签与生成产物的信号峰，基于这一性质，可利用该氟探针从原子分辨水平进行细胞内GSH的定量检测并进行GSH变化的动态监测；氟探针具有较好的稳定性，很好的细胞膜穿透性以及较高的巯基选择性，并对细胞无毒。
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