An oil production microbial agent production device and method
By designing a microbial agent production device with partitioned fermentation chambers and stirring components, the problems of long fermentation time and insufficient mixing of microorganisms were solved, achieving efficient fermentation and mixing of multiple microorganisms and improving crude oil recovery.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- SHAANXI INST OF BIOLOGICAL AGRI
- Filing Date
- 2023-06-16
- Publication Date
- 2026-07-31
AI Technical Summary
Existing microbial agent production equipment suffers from problems such as long microbial fermentation time, insufficient mixing, and easy mutual killing or inhibition of different microbial species.
A production device including a fermentation mechanism, a mixing mechanism, and an encapsulation mechanism was designed. It adopts a partitioned fermentation chamber and a stirring component. The fermentation cylinder is rotated by a drive component. Combined with a moving component and a tossing component, it can realize the simultaneous fermentation and mixing of multiple microorganisms and avoid direct contact between strains.
It improves the efficiency and mixing effect of microbial fermentation, reduces the probability of mutual killing or inhibition between strains, enhances the contact effect of fermentation liquid, and improves the recovery rate of crude oil.
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Figure CN116836785B_ABST
Abstract
Description
Technical Field
[0001] This invention relates to the field of microbial agent production equipment technology, specifically to an oilfield microbial agent production equipment and method. Background Technology
[0002] Microbial enhanced oil recovery (MEOR) refers to the method of extracting residual crude oil during the oil extraction process using microorganisms and their metabolic products. Microorganisms are small in size, grow rapidly, and have the advantages of being resistant to harsh formation environments such as high temperature, high salinity, and high pressure. They can also improve crude oil recovery rates through their metabolic products, such as gases, organic acids, surfactants, and polymers.
[0003] Most microbial agent production facilities involve long fermentation times, and liquid fermentation can only achieve simple mixing, failing to differentiate between different microorganisms and potentially leading to mutual killing or inhibition. Therefore, this paper proposes a production device and method for oilfield microbial agents using mixed fermentation. Summary of the Invention
[0004] To solve the above-mentioned technical problems, the present invention provides an apparatus and method for producing oilfield microbial agents.
[0005] The technical solution of the present invention is: an oilfield microbial agent production device, comprising a fermentation mechanism, a mixing mechanism, and an encapsulation mechanism, wherein the fermentation mechanism is connected to the mixing mechanism at its lower part, and the mixing mechanism is connected to the encapsulation mechanism at its lower part;
[0006] The fermentation mechanism includes a horizontally placed fermentation cylinder, a drive assembly, and a stirring assembly. The fermentation cylinder is equipped with a baffle to divide it into five fermentation chambers. The five fermentation chambers are a first aerobic fermentation chamber, a second aerobic fermentation chamber, and a third aerobic fermentation chamber for mixed aerobic fermentation, and a second anaerobic fermentation chamber for mixed anaerobic fermentation. Each fermentation chamber is equipped with an inlet for microorganisms to enter the fermentation chamber and an outlet for microorganisms to exit.
[0007] Both sides of the fermentation cylinder are provided with brackets for supporting the fermentation cylinder. The two ends of the fermentation cylinder are rotatably connected to the support shafts provided on the two brackets. The drive assembly includes a motor and a gear ring. The motor is provided on one of the brackets, and the gear ring is provided at one end of the fermentation cylinder. The output shaft of the motor is provided with a first gear that meshes with the gear ring for transmission.
[0008] The stirring assembly includes multiple stirring shafts arranged along the length of the fermentation tank, a round rod arranged vertically, and a toggle assembly for rotating the round rod. The round rod is rotatably connected to a support plate located on the end face of the fermentation tank between two of the stirring shafts. A second gear is provided on the support shaft located directly above the motor. Two sets of moving components are provided on the round rod. The two sets of moving components are symmetrically arranged about the round rod as an axis of symmetry. The two sides of the round rod are respectively connected to the stirring shafts on both sides of the round rod through the moving components.
[0009] The moving component includes a third gear that meshes with the second gear and a first connecting rod. The third gear is slidably connected to the stirring shaft via a sleeve. One end of the first connecting rod is rotatably connected to the sleeve, and the other end of the first connecting rod is fixedly connected to a round rod.
[0010] Description: This invention, through the inclusion of a fermentation device, a mixing mechanism, and an encapsulation mechanism, enables microorganisms to undergo liquid fermentation within a fermentation tank for strain propagation. The microorganisms are then mixed in a specific ratio using the mixing mechanism, and finally, the encapsulation mechanism produces a microbial agent to maintain viable cell counts during transportation and storage, while also facilitating oil extraction operations. The inclusion of a drive assembly and fermentation tank allows for the simultaneous fermentation of multiple microorganisms. During fermentation, the drive mechanism rotates the fermentation tank, ensuring thorough contact between the microorganisms and the fermentation liquid, promoting complete mixing and fermentation. The inclusion of a stirring shaft, a cylindrical rod, and a moving assembly allows the rotating fermentation tank to drive the stirring shaft within the fermentation chamber, enhancing the mixing of microorganisms and the fermentation liquid, thus improving the effectiveness of microbial fermentation and the mixed fermentation of multiple microorganisms. The inclusion of the moving assembly and actuating assembly allows the stirring shafts in different fermentation chambers to rotate alternately, stirring different microorganisms and improving the overall mixed fermentation effect.
[0011] Furthermore, the baffle between the first aerobic fermentation chamber and the second aerobic fermentation chamber is provided with multiple round holes, the baffle between the third aerobic fermentation chamber and the second aerobic fermentation chamber is provided with multiple round holes, and the baffle between the first anaerobic fermentation chamber and the second anaerobic fermentation chamber is provided with multiple round holes. Each round hole is provided with a membrane layer for the flow of fermentation liquid and gas.
[0012] Explanation: The fermentation liquid and gas in the first, second, and third aerobic fermentation chambers can be mixed through the circular holes, while the microorganisms in the first, second, and third aerobic fermentation chambers will not mix. The mixed fermentation of this device can simultaneously produce multiple microbial agents, making full use of the fermentation liquid and time. Different agents do not come into direct contact, reducing the probability of mutual killing or inhibition.
[0013] Furthermore, there are three stirring shafts, which are respectively located in the first aerobic fermentation chamber, the second aerobic fermentation chamber, and the third aerobic fermentation chamber. One end of each stirring shaft passes through the end face of the fermentation cylinder and is rotatably and sealed to it. One end of each of the two stirring shafts located in the first aerobic fermentation chamber and the second aerobic fermentation chamber is connected to two sets of moving components.
[0014] One end of the stirring shaft located in the third aerobic fermentation chamber is connected to the stirring shaft of the second aerobic fermentation chamber via a belt pulley assembly.
[0015] Note: The first, second, and third aerobic fermentation chambers are all equipped with stirring shafts to improve the contact between microorganisms, oxygen, and fermentation broth in the three fermentation chambers. Two sets of moving components allow the stirring rods in the first and second aerobic fermentation chambers to stir alternately, while the belt pulley assembly allows the stirring rod in the third aerobic fermentation chamber to also stir, thereby improving the inoculum cultivation effect in the three fermentation chambers.
[0016] Furthermore, the pulley assembly is located between the moving component and the fermentation tank. The pulley assembly includes a first pulley, a second pulley, and a belt. The first pulley is disposed on the stirring shaft of the third aerobic fermentation chamber, and the second pulley is disposed on the stirring shaft of the second aerobic fermentation chamber. The first pulley and the second pulley are connected by the belt drive.
[0017] Explanation: Through components such as the first and second pulleys, the stirring shaft of the second aerobic fermentation chamber rotates and stirs, while simultaneously driving the stirring shaft of the third aerobic fermentation chamber to stir as well, reducing the number of motors required, saving energy, and shortening fermentation time.
[0018] Furthermore, stirring blades are provided on the three stirring shafts corresponding to the positions of the circular holes.
[0019] Explanation: While the stirring blades agitate the fermentation liquid, they also intermittently scrape the diaphragm layer at the round holes, reducing the probability of clogging and improving the effect of mixed fermentation.
[0020] Furthermore, the first aerobic fermentation chamber is used to cultivate Pseudomonas aeruginosa, the second aerobic fermentation chamber is used to cultivate Bacillus subtilis, the third aerobic fermentation chamber is used to cultivate brewer's yeast, the first anaerobic fermentation chamber is used to cultivate Clostridium butyricum, and the second anaerobic fermentation chamber is used to cultivate Methanobacterium formate.
[0021] Note: Pseudomonas aeruginosa can produce the surfactant rhamnolipid, Bacillus subtilis can produce polyglutamic acid macromolecules, Clostridium butyricum can produce butyric acid, Methanobacterium formate can produce methane, and Saccharomyces cerevisiae can degrade heavy oil.
[0022] Furthermore, the oil-producing microbial agent is a mixture of any 3 to 5 of the following in any proportion: Pseudomonas aeruginosa, Bacillus subtilis, Saccharomyces cerevisiae, Clostridium butyricum, and Methanobacterium formate.
[0023] Note: Microorganisms are small in size, grow rapidly, and have the advantages of being resistant to harsh geological environments such as high temperature, high salinity, and high pressure. They can also improve crude oil recovery through their metabolic products, such as gases, organic acids, surfactants, and polymers.
[0024] Furthermore, the actuating assembly includes a second connecting rod, a third connecting rod, and a shaft. One end of the second connecting rod is rotatably connected to the shaft. A fourth connecting rod is provided on the shaft located above the second connecting rod, with one end rotatably connected to the shaft. The other end of the fourth connecting rod is fixedly connected to the middle of the third connecting rod. The middle of the third connecting rod is connected to the middle of the second connecting rod by a spring. Two levers for actuating the two ends of the third connecting rod are provided on the fermentation cylinder located above both sides of the support plate.
[0025] The bottom of the second connecting rod, which corresponds to the edge of the shaft end face, is provided with a sliding rod. The upper end of the round rod is provided with a groove for the sliding rod to rotate the round rod. The bottom of the shaft is fixedly connected to the support member provided on the bracket.
[0026] Explanation: The moving component does not rotate with the fermentation tank. Two levers on the fermentation tank intermittently actuate the two ends of the third connecting rod. This, in conjunction with the fourth connecting rod and springs, causes the second connecting rod to intermittently swing left or right. This, in conjunction with the sliding rod and grooved parts, drives the round rod to rotate. This intermittently causes the two third gears to alternately mesh with the gear ring, thus allowing the stirring shaft to rotate while the fermentation tank rotates. This stirs the first, second, and third aerobic fermentation chambers, further improving the mixing and contact effect between the microbial strains, fermentation liquid, and oxygen.
[0027] The method for producing microbial agents using the above-mentioned production apparatus includes the following steps:
[0028] S1. Fermentation preparation:
[0029] The oxygen concentration in the first and second anaerobic fermentation chambers of the fermentation tank is reduced to an anaerobic state. Then, the required fermentation liquid is added to each fermentation chamber through the inlet. Finally, Pseudomonas aeruginosa, Bacillus subtilis, and brewer's yeast are added to the first, second, and third aerobic fermentation chambers respectively, and Clostridium butyricum and Methanobacterium formate are added to the first and second anaerobic fermentation chambers respectively.
[0030] S2, Fermentation and Expansion:
[0031] When the motor is started, the first gear on the output shaft rotates. The gear ring on the fermentation tank meshes with the first gear, causing the fermentation tank to rotate. This causes the microorganisms and fermentation liquid inside the fermentation tank to shake and come into full contact. When the toggle component is used to rotate the round rod clockwise, the round rod drives the first connecting rod on the two sets of moving components to rotate. This causes the third gear on one set of moving components to slide along the stirring shaft and mesh with the second gear. The third gear then rotates around the stirring shaft, ultimately driving the corresponding stirring shaft to rotate. The stirring shaft stirs the microorganisms and fermentation liquid in the fermentation chamber. When the toggle component is used to rotate the round rod counterclockwise, the stirring shaft connected to the other set of moving components rotates.
[0032] S3. Preparation of bacterial agent:
[0033] The fermented and expanded microorganisms are discharged through the outlet, then filtered, and then added to the mixing mechanism in a certain proportion for mixing. The mixed microorganisms are then added to the encapsulation mechanism to make a microcapsule-based oil recovery microbial agent.
[0034] The beneficial effects of this invention are:
[0035] (1) This invention uses a fermentation device, a mixing mechanism and an encapsulation mechanism to ferment and mix microorganisms to make microcapsule inoculants. By setting up a driving component and a fermentation cylinder, multiple microorganisms can be fermented simultaneously. The driving mechanism drives the fermentation cylinder to rotate, so that the microorganisms and fermentation liquid inside can come into full contact. By setting up a stirring shaft, a round rod and a moving component, the stirring shaft can be driven to stir in the fermentation chamber when the fermentation cylinder rotates, thereby improving the effect of microbial fermentation and mixed fermentation of multiple microorganisms. By setting up a moving component and a toggle component, the stirring shafts of different fermentation chambers can rotate alternately, thereby improving the mixed fermentation effect.
[0036] (2) The moving component of the present invention does not rotate together with the fermentation tank. Through the two levers on the fermentation tank, the two ends of the third link can be intermittently moved. In conjunction with the fourth link and springs, the second link can be intermittently swung to the left or right. In conjunction with the sliding rod and groove, the two third gears can be intermittently meshed with the gear ring for transmission, so that the stirring shafts of different fermentation chambers can be stirred alternately, further improving the mixing and contact of microbial strains with fermentation liquid and oxygen.
[0037] (3) The microbial agent of the present invention can improve the recovery rate of crude oil. Pseudomonas aeruginosa can produce surfactant rhamnolipin, Bacillus subtilis can produce polyglutamic acid macromolecules, Clostridium butyricum can produce butyric acid, Methanobacterium formate can produce methane, and Saccharomyces cerevisiae can degrade heavy oil. The microbial liquid fermentation method is used to expand the microbial strain, and then it is prepared according to the proportion. Then, the microbial strain is encapsulated using high-tech means to make a microcapsule oil recovery microbial agent, so as to maintain the number of live bacteria during transportation and storage, and at the same time facilitate the operation of oil recovery. Attached Figure Description
[0038] Figure 1 This is a schematic diagram of the external appearance of the production apparatus of the present invention;
[0039] Figure 2 This is a top view of the production apparatus of the present invention;
[0040] Figure 3 This is a schematic diagram of the internal structure of the fermentation tank of the present invention;
[0041] Figure 4 This is a schematic diagram of the structure of the baffle of the present invention;
[0042] Figure 5 This is a schematic diagram of the drive mechanism of the present invention;
[0043] Figure 6 This is the present invention. Figure 1 Enlarged view of point A;
[0044] Figure 7 This is the present invention. Figure 2 Enlarged view of point B;
[0045] Figure 8 This is a schematic diagram of the appearance of the production apparatus in Embodiment 2 of the present invention;
[0046] Figure 9 This is a schematic diagram showing the position of the toggle component in Embodiment 2 of the present invention;
[0047] Figure 10 This is the present invention. Figure 9 Top view;
[0048] Figure 11 This is the present invention. Figure 8 Enlarged view of point E;
[0049] Figure 12 This is a schematic diagram showing the positional relationship between the shaft and the third connecting rod of the present invention;
[0050] Figure 13 This is the present invention. Figure 10 Enlarged view of point D;
[0051] Figure 14 This is the present invention. Figure 9 Enlarged view of point C;
[0052] Figure 15 This is a schematic diagram showing the position of the card in Embodiment 3 of the present invention;
[0053] Figure 16 This is a schematic diagram showing the positional relationship between the card and the second connecting rod of the present invention;
[0054] Figure 17 This is a schematic diagram of the structure of the card component of the present invention;
[0055] Among them, 1-fermentation mechanism, 11-fermentation cylinder, 111-baffle, 112-first aerobic fermentation chamber, 113-second aerobic fermentation chamber, 114-third aerobic fermentation chamber, 115-first anaerobic fermentation chamber, 116-second anaerobic fermentation chamber, 117-support, 118-support shaft, 12-drive mechanism, 121-motor, 122-gear ring, 123-first gear, 2-clamping component, 3-stirring assembly, 31-stirring shaft, 32-round rod. 321-Support plate, 33-Actuating assembly, 331-Second link, 332-Third link, 333-Shaft, 334-Fourth link, 335-Spring, 336-Slide rod, 337-Groove, 338-Actuating lever, 34-Second gear, 35-Moving assembly, 351-Third gear, 352-First link, 353-Sleeve, 36-Pulley assembly, 361-First pulley, 362-Second pulley, 363-Belt. Detailed Implementation
[0056] The present invention will now be described in more detail with reference to specific embodiments, so as to better demonstrate the advantages of the present invention.
[0057] Example 1
[0058] like Figure 1 As shown, an oilfield microbial agent production device includes a fermentation unit 1, a mixing unit, and an encapsulation unit. The fermentation unit 1 is connected to the mixing unit at its lower part, and the mixing unit is connected to the encapsulation unit at its lower part. The mixing unit and the encapsulation unit are made of commercially available mixing tanks and encapsulation machines, or their shapes are adjusted according to commercially available mixing tanks and encapsulation machines to be adapted for installation in this device.
[0059] like Figure 1 , 3As shown, the fermentation mechanism 1 includes a fermentation cylinder 11 placed horizontally, a drive assembly 12, and a stirring assembly 3. The fermentation cylinder 11 is provided with a baffle 111 for dividing it into five fermentation chambers. The five fermentation chambers are a first aerobic fermentation chamber 112, a second aerobic fermentation chamber 113, and a third aerobic fermentation chamber 114 for aerobic mixed fermentation, and a first anaerobic fermentation chamber 115 and a second anaerobic fermentation chamber 116 for mixed anaerobic fermentation. Each fermentation chamber is provided with an inlet for microorganisms to enter the fermentation chamber and an outlet for microorganisms to exit.
[0060] like Figure 2 , 5 As shown, both sides of the fermentation cylinder 11 are provided with brackets 117 for supporting the fermentation cylinder 11. The two ends of the fermentation cylinder 11 are rotatably connected to the support shafts 118 provided on the two brackets 117. The drive assembly 12 includes a motor 121 and a gear ring 122. The motor 121 is provided on the right bracket 117, and the gear ring 122 is provided on the right end of the fermentation cylinder 11. The output shaft of the motor 121 is provided with a first gear 123 that meshes with the gear ring 122 for transmission. The motor 121 is a commercially available rotary motor or a commercially available rotary motor with an appearance adjusted to fit this device.
[0061] like Figure 6 , 7 As shown, the stirring assembly 3 includes three stirring shafts 31 arranged along the length of the fermentation tank 11, a round rod 32 arranged in the vertical direction, and a lever assembly 33 for rotating the round rod 32. The lever assembly 33 is a rotating plate. The round rod 32 is rotatably connected to a support plate 321 located on the end face of the fermentation tank 11 between the two stirring shafts 31. A second gear 34 is provided on the support shaft 118 located above the motor 121. Two sets of moving components 35 are provided on the round rod 32. The two sets of moving components 35 are symmetrically arranged about the round rod 32 as the axis of symmetry. The two sides of the round rod 32 are respectively connected to the stirring shafts 31 on both sides of the round rod 32 through the moving components 35.
[0062] like Figure 7 As shown, the moving component 35 includes a third gear 351 that meshes with the second gear 34 and a first connecting rod 352. The third gear 351 is slidably connected to a fixed rod provided at the right end of the stirring shaft 31 through a sleeve 353. One end of the first connecting rod 352 is rotatably connected to the sleeve 353, and the other end of the first connecting rod 352 is fixedly connected to the round rod 32.
[0063] like Figure 3 , 4As shown, the baffle 111 between the first aerobic fermentation chamber 112 and the second aerobic fermentation chamber 113 is provided with a plurality of round holes, the baffle 111 between the third aerobic fermentation chamber 114 and the second aerobic fermentation chamber 113 is provided with a plurality of round holes, and the baffle 111 between the first anaerobic fermentation chamber 115 and the second anaerobic fermentation chamber 116 is provided with a plurality of round holes. Each of the round holes is provided with a membrane layer for the flow of fermentation liquid and gas, and the membrane layer is a commercially available filter membrane.
[0064] There are three stirring shafts 31, which are located in the first aerobic fermentation chamber 112, the second aerobic fermentation chamber 113 and the third aerobic fermentation chamber 114 respectively; each of the three stirring shafts 31 corresponding to the position of the round hole is equipped with stirring blades.
[0065] The first aerobic fermentation chamber 112 is used to cultivate Pseudomonas aeruginosa, the second aerobic fermentation chamber 113 is used to cultivate Bacillus subtilis, the third aerobic fermentation chamber 114 is used to cultivate brewer's yeast, the first anaerobic fermentation chamber 115 is used to cultivate Clostridium butyricum, and the second anaerobic fermentation chamber 116 is used to cultivate Methanobacterium formate.
[0066] The oil recovery microbial agent is composed of Pseudomonas aeruginosa, Bacillus subtilis, Saccharomyces cerevisiae, Clostridium butyricum, and Methanobacterium formate in a weight ratio of 1:1:1:1:1;
[0067] like Figure 2 , 7 As shown, the right end of the stirring shaft 31 passes through the end face of the fermentation cylinder 11 and is rotatably and sealed to it, and the left end of the stirring shaft 31 is rotatably connected to the bottom of the fermentation cylinder 11. The right ends of the two stirring shafts 31 located in the first aerobic fermentation chamber 112 and the second aerobic fermentation chamber 113 are respectively connected to the two sets of moving components 35 by belt drive.
[0068] like Figure 7 As shown, the right end of the stirring shaft 31 located in the third aerobic fermentation chamber 114 is connected to the right end of the stirring shaft 31 in the second aerobic fermentation chamber 113 via a pulley assembly 36.
[0069] like Figure 6 , 7 As shown, the pulley assembly 36 is located between the moving component 35 and the fermentation tank 11. The pulley assembly 36 includes a first pulley 361, a second pulley 362, and a belt 363. The first pulley 361 is mounted on the stirring shaft 31 of the third aerobic fermentation chamber 114, and the second pulley 362 is mounted on the stirring shaft 31 of the second aerobic fermentation chamber 113. The first pulley 361 and the second pulley 362 are connected by the belt 363.
[0070] The method for producing microbial agents using the aforementioned oilfield microbial agent production device includes the following steps:
[0071] S1. Fermentation preparation:
[0072] The oxygen concentration in the first anaerobic fermentation chamber 115 and the second anaerobic fermentation chamber 116 in the fermentation tank 11 is reduced to an anaerobic state. Specifically, the air in the first anaerobic fermentation chamber 115 and the second anaerobic fermentation chamber 116 is extracted with a vacuum pump and high-purity nitrogen is introduced. This process is repeated three times. Then, the required fermentation liquid is added to each fermentation chamber through the inlet. Finally, Pseudomonas aeruginosa, Bacillus subtilis, and brewer's yeast are added to the first aerobic fermentation chamber 112, the second aerobic fermentation chamber 113, and the third aerobic fermentation chamber 114, respectively. Clostridium butyricum and Methanobacterium formate are added to the first anaerobic fermentation chamber 115 and the second anaerobic fermentation chamber 116, respectively.
[0073] S2, Fermentation and Expansion:
[0074] When motor 121 is started, it drives the first gear 123 on the output shaft to rotate. The gear ring 122 on the fermentation tank 11 meshes with the first gear 123, causing the fermentation tank 11 to rotate. This causes the microorganisms and fermentation liquid inside the fermentation tank 11 to agitate and come into full contact. When the actuating component 33 is used to rotate the round rod 11 clockwise, the round rod 11 drives the first connecting rod 352 on the two sets of moving components 35 to rotate around the round rod 11. This causes the third gear 351 on one set of moving components 35 to slide along the stirring shaft 31 and mesh with the second gear 34. This causes the third gear 351 to drive the stirring shaft 31 to rotate, ultimately causing the first... The stirring shaft 31 of the aerobic fermentation chamber 112 rotates, stirring the microorganisms and fermentation liquid in the first aerobic fermentation chamber 112. When the rotary rod 11 is rotated counterclockwise using the toggle assembly 33, the stirring shaft 31 of the second aerobic fermentation chamber 113 rotates. At the same time, the first pulley 361 on the stirring shaft 31 drives the second pulley 362 to rotate via the belt 363, ultimately driving the stirring shaft 31 of the third aerobic fermentation chamber 114 to rotate. During stirring, oxygen is introduced into the first aerobic fermentation chamber 112, the second aerobic fermentation chamber 113, and the third aerobic fermentation chamber 114. After stirring, the motor 121 is turned off.
[0075] S3. Preparation of bacterial agent:
[0076] The fermented and expanded microorganisms are discharged through the outlet and then filtered. Subsequently, Pseudomonas aeruginosa, Bacillus subtilis, brewer's yeast, Clostridium butyricum and Methanobacterium formate are added to the mixing mechanism in a weight ratio of 1:1:1:1:1 and mixed. The mixed microorganisms are then added to the encapsulation mechanism to produce a microcapsule-based oil recovery microbial agent.
[0077] Example 2
[0078] The difference between this embodiment and Embodiment 1 is that, Figure 10 , 12 As shown in Figure 13, the actuating assembly 33 includes a second connecting rod 331, a third connecting rod 332, and a shaft 333. The left end of the second connecting rod 331 is rotatably connected to the shaft 333. A fourth connecting rod 334 is provided on the shaft 333 above the second connecting rod 331, with its right end rotatably connected to the shaft 333. The left end of the fourth connecting rod 334 is fixedly connected to the middle part of the third connecting rod 332. The middle part of the third connecting rod 332 is connected to the middle part of the second connecting rod 331 by a spring 335.
[0079] like Figure 9 , 14 As shown, the fermentation cylinder 11 located above both sides of the support plate 321 is provided with two levers 338 for moving the two ends of the third connecting rod 332.
[0080] like Figure 8 , 11 As shown, the bottom of the second connecting rod 331, located at the position corresponding to the edge of the end face of the shaft 333 (5cm from the axis), is provided with a sliding rod 336. The upper end of the round rod 32 is provided with a groove 337 for the sliding rod 336 to rotate the round rod 32. The bottom of the shaft 333 is connected to the support member 119 (such as...) provided on the bracket 117. Figure 12 (Structure shown) Fixed connection;
[0081] The operation method of the above-mentioned device is as follows:
[0082] The position of the toggle component 33 is as follows Figure 8 , 11As shown, the third connecting rod 332 tilts to the left, and the second connecting rod 331 tilts to the left. When the fermentation cylinder 11 rotates, the lever 338 on it also rotates. When the right lever 338 contacts the right end of the third connecting rod 332 and continuously pushes the right end of the third connecting rod 332, the round rod 32 rotates to a vertical position, and the shaft 333 is located directly above the round rod 32. At the same time, the sliding rod 336 enters the groove in the groove part 337. The fermentation cylinder 11 continues to rotate, and the lever 338 pushes the third connecting rod 332 to rotate clockwise around the shaft 333. The third connecting rod 332 tilts to the right, and the spring 335 is stretched. When the lever 338... When not in contact with the right end of the third connecting rod 332, the spring 335 retracts, causing the second connecting rod 331 to rotate counterclockwise around the shaft 333. The second connecting rod 331 tilts to the right, and the slide rod 331 rotates with the second connecting rod 331, causing the grooved part 337 to rotate. The grooved part 337 drives the round rod 32 to rotate counterclockwise, causing the other set of moving components 35 to drive the second gear 34 to rotate, driving the stirring shaft 31 of the second aerobic fermentation chamber 113 to rotate. The second grooved part 337 rotates together with the fermentation cylinder 11, so that after the slide rod 331 drives the grooved part 337 to rotate, it moves out of the groove of the grooved part 337. Figure 13 , 14 As shown; after the fermentation tank 11 rotates one revolution, the left lever 338 contacts the right end of the third connecting rod 332 and continuously pushes the right end of the third connecting rod 332. The principle is the same as above, causing the stirring shaft 31 of the first aerobic fermentation chamber 112 to rotate and continuously stir intermittently.
[0083] Example 3
[0084] This embodiment is basically the same as embodiment 2, except that, as Figure 15 , 16 As shown in Figure 17, a locking member 2 for restricting the rotation of the second link 331 is provided on the support member 119 located directly below the second link 331. The locking member 2 is rotatably connected to the support member 119.
[0085] The operation method of the above-mentioned device is as follows:
[0086] like Figure 16 As shown, rotating the locking piece 2 causes it to contact the second connecting rod 331, making the second connecting rod 331 horizontal and not tilted. This makes the moving component 35 vertical, preventing the two third gears 351 from contacting the second gear 34. The three stirring shafts 31 do not rotate. Rotating the locking piece 2 does not restrict the rotation of the second connecting rod 331. Under the action of the spring 335, the second connecting rod 331 rotates around the shaft 333, causing the three stirring shafts 31 to rotate intermittently.
Claims
1. An apparatus for producing oilfield microbial inoculants, characterized in that, It includes a fermentation mechanism (1), a mixing mechanism, and an encapsulation mechanism. The fermentation mechanism (1) is connected to the mixing mechanism at its lower part, and the mixing mechanism is connected to the encapsulation mechanism at its lower part. The fermentation mechanism (1) includes a fermentation cylinder (11) placed horizontally, a drive assembly (12) and a stirring assembly (3). The fermentation cylinder (11) is provided with a baffle (111) for dividing it into five fermentation chambers. The five fermentation chambers are a first aerobic fermentation chamber (112), a second aerobic fermentation chamber (113) and a third aerobic fermentation chamber (114) for mixed aerobic fermentation, and a first anaerobic fermentation chamber (115) and a second anaerobic fermentation chamber (116) for mixed anaerobic fermentation. Each fermentation chamber is provided with an inlet for microorganisms to enter the fermentation chamber and an outlet for microorganisms to exit. The fermentation cylinder (11) is provided with brackets (117) on both sides for supporting the fermentation cylinder (11). The two ends of the fermentation cylinder (11) are rotatably connected to the support shafts (118) provided on the two brackets (117). The drive assembly (12) includes a motor (121) and a gear ring (122). The motor (121) is provided on one of the brackets (117), and the gear ring (122) is provided at one end of the fermentation cylinder (11). The output shaft of the motor (121) is provided with a first gear (123) that meshes with the gear ring (122). The stirring assembly (3) includes multiple stirring shafts (31) arranged along the length of the fermentation cylinder (11), a round rod (32) arranged in the vertical direction, and a toggle assembly (33) for rotating the round rod (32). The round rod (32) is rotatably connected to a support plate (321) provided on the end face of the fermentation cylinder (11) between the two stirring shafts (31). A second gear (34) is provided on the support shaft (118) located directly above the motor (121). Two sets of moving assemblies (35) are provided on the round rod (32). The two sets of moving assemblies (35) are symmetrically arranged with the round rod (32) as the axis of symmetry. The two sides of the round rod (32) are respectively connected to the stirring shafts (31) on both sides of the round rod (32) through the moving assemblies (35). The moving component (35) includes a third gear (351) that meshes with the second gear (34) and a first connecting rod (352). The third gear (351) is slidably connected to the stirring shaft (31) through a sleeve (353). One end of the first connecting rod (352) is rotatably connected to the sleeve (353), and the other end of the first connecting rod (352) is fixedly connected to the round rod (32). The baffle (111) between the first aerobic fermentation chamber (112) and the second aerobic fermentation chamber (113) is provided with a plurality of round holes, the baffle (111) between the third aerobic fermentation chamber (114) and the second aerobic fermentation chamber (113) is provided with a plurality of round holes, and the baffle (111) between the first anaerobic fermentation chamber (115) and the second anaerobic fermentation chamber (116) is provided with a plurality of round holes, and each of the round holes is provided with a membrane layer for the flow of fermentation liquid and gas; There are three stirring shafts (31), which are located in the first aerobic fermentation chamber (112), the second aerobic fermentation chamber (113) and the third aerobic fermentation chamber (114) respectively. One end of the stirring shaft (31) passes through the end face of the fermentation cylinder (11) and is rotatably sealed to it. One end of the two stirring shafts (31) located in the first aerobic fermentation chamber (112) and the second aerobic fermentation chamber (113) is connected to two sets of moving components (35) respectively. One end of the stirring shaft (31) located in the third aerobic fermentation chamber (114) is connected to the stirring shaft (31) of the second aerobic fermentation chamber (113) via a belt drive through a pulley assembly (36); The pulley assembly (36) is located between the moving assembly (35) and the fermentation tank (11). The pulley assembly (36) includes a first pulley (361), a second pulley (362), and a belt (363). The first pulley (361) is mounted on the stirring shaft (31) of the third aerobic fermentation chamber (114), and the second pulley (362) is mounted on the stirring shaft (31) of the second aerobic fermentation chamber (113). The first pulley (361) and the second pulley (362) are connected by belt drive through the belt (363).
2. The oilfield microbial agent production device as described in claim 1, characterized in that, Each of the three stirring shafts (31) corresponding to the position of the circular hole is provided with stirring blades.
3. The oilfield microbial agent production device as described in claim 1, characterized in that, The first aerobic fermentation chamber (112) is used to cultivate Pseudomonas aeruginosa, the second aerobic fermentation chamber (113) is used to cultivate Bacillus subtilis, the third aerobic fermentation chamber (114) is used to cultivate brewer's yeast, the first anaerobic fermentation chamber (115) is used to cultivate Clostridium butyricum, and the second anaerobic fermentation chamber (116) is used to cultivate Methanobacterium formate.
4. The oilfield microbial agent production device as described in claim 3, characterized in that, The oil recovery microbial agent is a mixture of any 3 to 5 of the following: Pseudomonas aeruginosa, Bacillus subtilis, Saccharomyces cerevisiae, Clostridium butyricum, and Methanobacterium formate, in any proportion.
5. The oilfield microbial agent production device as described in claim 1, characterized in that, The actuating assembly (33) includes a second connecting rod (331), a third connecting rod (332), and a shaft (333). One end of the second connecting rod (331) is rotatably connected to the shaft (333). A fourth connecting rod (334) is provided on the shaft (333) located above the second connecting rod (331), with one end rotatably connected to the shaft (333). The other end of the fourth connecting rod (334) is fixedly connected to the middle of the third connecting rod (332). The middle of the third connecting rod (332) is connected to the middle of the second connecting rod (331) by a spring (335). Two levers (338) for actuating the two ends of the third connecting rod (332) are provided on the fermentation cylinder (11) located above both sides of the support plate (321). The bottom of the second connecting rod (331) corresponding to the edge of the end face of the shaft (333) is provided with a sliding rod (336), and the upper end of the round rod (32) is provided with a groove (337) for the sliding rod (336) to rotate the round rod (32). The bottom of the shaft (333) is fixedly connected to the support member (119) provided on the bracket (117).
6. A method for producing microbial agents using the oilfield microbial agent production apparatus according to any one of claims 1 to 5, characterized in that, Includes the following steps: S1. Fermentation preparation: The oxygen concentration in the first anaerobic fermentation chamber (115) and the second anaerobic fermentation chamber (116) in the fermentation tank (11) is reduced to an anaerobic state. Then, the required fermentation liquid is added to each fermentation chamber through the inlet. Finally, Pseudomonas aeruginosa, Bacillus subtilis, and brewer's yeast are added to the first aerobic fermentation chamber (112), the second aerobic fermentation chamber (113), and the third aerobic fermentation chamber (114), respectively. Clostridium butyricum and Methanobacterium formate are added to the first anaerobic fermentation chamber (115) and the second anaerobic fermentation chamber (116), respectively. S2, Fermentation and Expansion: When the motor (121) is started, the first gear (123) on the output shaft is driven to rotate. The gear ring (122) on the fermentation tank (11) meshes with the first gear (123) to drive the fermentation tank (11) to rotate, causing the microorganisms and fermentation liquid in the fermentation tank (11) to shake and come into full contact. When the toggle assembly (33) is used to rotate the round rod (32) clockwise, the round rod (32) drives the first connecting rod (352) on the two sets of moving assemblies (35) to rotate, causing the third gear (351) on one set of moving assemblies (35) to slide along the stirring shaft (31) to mesh with the second gear (34), causing the third gear (351) to rotate around the stirring shaft (31), and finally drive the corresponding stirring shaft (31) to rotate. The stirring shaft (31) stirs the microorganisms and fermentation liquid in the fermentation chamber. When the toggle assembly (33) is used to rotate the round rod (32) counterclockwise, the stirring shaft (31) connected to the other set of moving assemblies (35) rotates. S3. Preparation of bacterial agent: The fermented and expanded microorganisms are discharged through the outlet, then filtered, and then added to the mixing mechanism in a certain proportion for mixing. The mixed microorganisms are then added to the encapsulation mechanism to make a microcapsule-based oil recovery microbial agent.