用于单管闭管检测一组肠道微生物的2D-PCR方法
The 2D-PCR method enables the simultaneous detection of multiple gut microbiota in a single tube, solving the problems of low detection efficiency and high cost in existing technologies. It achieves high-throughput, low-cost gut microbiota detection and has high scientific application value.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- LONGHUA HOSPITAL SHANGHAI UNIV OF TRADITIONAL CHINESE MEDICINE
- Filing Date
- 2022-03-25
- Publication Date
- 2026-07-17
AI Technical Summary
Existing technologies are difficult to use for efficient detection of multiple gut microbiota in a single tube, and they also suffer from cross-reactivity and high detection costs.
A two-dimensional PCR (2D-PCR) method based on base quenching probe technology was adopted. By adding different tag sequences to the 5' end of the microorganism-specific upstream primer and combining the dissolution temperature difference of the fluorescent probe, the simultaneous detection of multiple microorganisms in a single tube system was achieved.
It enables high-throughput, low-cost detection of a variety of gut microbiota, avoids cross-reactions, simplifies operation procedures, reduces the risk of laboratory contamination, and improves the accuracy and efficiency of detection.
Smart Images
Figure CN116837118B_ABST