Fructose probe binding protein Tom22 and screening method and application thereof

Abstract

The application discloses a fructose probe binding protein Tom22 and a screening method and application thereof. The screening method of the fructose probe binding protein Tom22 comprises the following steps: (1) designing and synthesizing a fructose probe capable of "fishing" a fructose direct binding protein through ultraviolet crosslinking and click chemistry reaction; (2) based on ABPP technology, using the fructose probe to identify the fructose binding protein in in-vitro cultured podocytes, and covalently labeling the binding protein through ultraviolet crosslinking; (3) after the probe-labeled protein is enriched through click chemistry reaction, a non-labeled quantitative mass spectrometry technology is used to screen and identify potential fructose direct binding proteins. In the candidate fructose binding proteins, the mitochondrial TOM complex subunit Tom22 is a protein with the largest number of identified peptide segments and the highest coverage.

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