Bnac05.uk gene for regulating oil content of rape and application thereof
Through genome-wide association analysis and metabolomics analysis, the BnaC05.UK loss-of-function mutant was discovered and created using CRISPR/Cas9 technology, solving the technical challenge of regulating rapeseed oil content and achieving a significant increase in rapeseed seed oil content.
CN116855506BActive Publication Date: 2026-07-21HUAZHONG AGRI UNIV
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- HUAZHONG AGRI UNIV
- Filing Date
- 2023-06-09
- Publication Date
- 2026-07-21
Smart Images
Figure CN116855506B_ABST
Abstract
The application discloses a BnaC05.UK gene for regulating oil content of rape, wherein the nucleotide sequence is shown as SEQ ID NO:1, and further discloses a protein coded by the gene, and a CRISPR / Cas9 gene editing carrier and a recombination bacteria of the gene. The CRISPR / Cas9 gene editing carrier of the BnaC05.UK gene is transformed into the genome of the rape by using an agrobacterium-mediated genetic transformation method, a BnaC05.UK gene function loss type rape variety is obtained, and the oil content of the mutant material is determined. The result shows that the BnaC05.UK gene negatively regulates the accumulation of the oil content of the rape, and the oil content of the mutant material is significantly increased compared with the wild type. The application has very important significance in high-oil breeding of the rape.
Need to check novelty before this filing date? Find Prior Art