A method of measuring the degree of curl of a pepper fruit

Abstract

The application discloses a method for measuring the spiral degree of pepper fruits, comprising the following steps: S1, cutting a middle fruit section with uniform spiral from the pepper fruits to be measured; S2, inserting a needle vertically to the surface of the fruits on the spiral lines of the fruit section to be measured every 1 cm in the direction perpendicular to the cross section of the fruits, and totally inserting n needles; S3, vertically fixing the fruit section to be measured on a detection table with a black background, meanwhile, placing a reference object, and using a camera with a lens perpendicular to the detection table to take a picture to obtain an original image; S4, correcting the lens distortion to obtain a corrected image; and S5, measuring the included angle phi between the first needle and the n-th needle in the corrected image to obtain the spiral angle RDM of the fruit section to be measured. The application combines the computer-aided measurement of the fruit shape, has the advantages of low cost and high precision, reduces the error of the spiral degree, and improves the calculation accuracy of the spiral angle of the pepper fruits.

Description

Technical Field

[0001] This invention relates to a method for measuring the spiral degree of chili pepper fruits. Background Technology

[0002] Chili peppers are an important global vegetable, and fruit shape is one of the key indicators of their appearance quality. Quantifying fruit shape is a prerequisite for conducting genetic analysis of fruit shape. Accurate and convenient measurement of fruit phenotypic traits is crucial in studies such as chili pepper germplasm resource classification, QTL mapping of fruit shape, cloning of fruit shape genes, and the mechanism of fruit shape formation. The screw pepper, named for its screw-like shape, has long, horn-shaped fruit with wrinkles at the base. It has a stronger spicy aroma, thinner skin, and crisper flesh, and grows in a right-handed spiral. In recent years, it has become increasingly popular with consumers.

[0003] Currently, research on the spiral shape of chili peppers is progressing slowly, largely because existing indicators describing fruit shape cannot fully cover chili pepper populations with unique fruit shape traits, making quantitative evaluation of these traits difficult. Therefore, developing a method to improve the accuracy of measuring the spiral shape of chili pepper fruits has become an urgent problem to be solved. Summary of the Invention

[0004] To address the current difficulties in quantitatively evaluating the degree of spirality in fruit measurement, this invention provides a method and system for measuring the spiral phenotype of chili pepper fruits, ensuring the objectivity and accuracy of fruit spiral phenotype data.

[0005] To solve the above-mentioned technical problems, the present invention provides a method for measuring the spiral degree of chili pepper fruits, comprising the following steps:

[0006] S1. Cut a uniform spiral middle segment from the pepper fruit to be tested to obtain the fruit segment to be tested;

[0007] S2. Insert a needle-like object perpendicular to the surface of the fruit on the spiral pattern of the fruit segment to be tested every 1 cm along the direction perpendicular to the cross-section of the fruit. Insert a total of n needles, where n is a natural number greater than or equal to 2.

[0008] S3. Fix the fruit segment to be tested vertically on the testing platform with a black background, and place a reference object at the same time. Use a camera with the lens perpendicular to the testing platform to take a picture to obtain the original image.

[0009] S4. Using computer image processing software, the lens distortion in the original image is corrected based on the reference object to obtain the corrected image;

[0010] S5. Using computer image processing software, measure the angle φ between the 1st and nth needle-like objects in the corrected image, and calculate the helix angle RDM of the fruit segment to be tested:

[0011] RDM = φ / (n-1)

[0012] The unit of RDM is °cm -1 .

[0013] In the method of this invention, lens distortion is corrected using Photoshop software.

[0014] In the method of this invention, the included angle φ is measured using ImageJ software.

[0015] In the method of the present invention, the needle-like object can be selected from pins, sewing needles, toothpicks, etc.

[0016] In the method of the present invention, the length of the intermediate fruit segment can be 3-6cm, preferably 4-5cm.

[0017] This invention discloses a method for measuring the spiral degree of chili pepper fruits, which, combined with a computer-aided fruit shape determination method, has the advantages of low cost and high precision, reduces the error of spiral degree, and improves the accuracy of calculating the spiral angle of chili pepper fruits. Attached Figure Description

[0018] Figure 1 This is a schematic diagram of the helix angle measurement in Embodiment 1 of the present invention. Detailed Implementation

[0019] The present invention will now be described in further detail with reference to specific embodiments. The given embodiments are merely illustrative of the invention and not intended to limit its scope. The embodiments provided below can serve as a guide for further improvements by those skilled in the art and do not constitute a limitation on the invention in any way.

[0020] Unless otherwise specified, the experimental methods used in the following examples are conventional methods, performed according to the techniques or conditions described in the literature in this field or according to the product instructions. Unless otherwise specified, the materials and reagents used in the following examples are commercially available.

[0021] Example 1

[0022] Measuring the spiral angle of a chili pepper fruit includes the following steps:

[0023] S1. Cut a uniform spiral-shaped middle segment from the pepper fruit to be tested, as follows:

[0024] Cut off the base and tail of the chili pepper fruit, leaving only the middle section of the fruit, which is about 4-5 cm long and spirally arranged in the middle, as the fruit section to be tested.

[0025] S2. Insert a pin perpendicular to the fruit surface along the spiral pattern of the fruit segment to be tested, every 1 cm along the direction perpendicular to the fruit's cross-section, as follows:

[0026] Mark the spiral pattern of the fruit segment to be tested with a marker every 1cm perpendicular to the cross-section of the fruit. Insert a pin perpendicular to the fruit surface at the marked position. Use 3-4 pins for each fruit segment.

[0027] S3. Fix the fruit segment to be tested vertically on the testing platform with a black background, and place a reference object at the same time. Use a camera with its lens perpendicular to the testing platform to take a picture to obtain the original image, as follows:

[0028] A bamboo skewer is vertically fixed on a black testing table, and the camera lens is perpendicular to the bamboo skewer and fixed in place; the fruit segment to be tested is placed vertically on the bamboo skewer, and a ruler and a disc are also placed on the black testing table as references and photographed to obtain the original image.

[0029] S4. Using computer image processing software, the lens distortion in the original image is corrected based on the reference object to obtain the corrected image:

[0030] Open the original image in Photoshop, select the layer with the mouse, select Filter, select Lens Correction, click the Remove Distortion button in the pop-up dialog box, drag it outward, and finally click OK;

[0031] S5. Use computer image processing software to measure the angle φ between the 1st and nth needles in the corrected image, and calculate the helix angle RDM of the fruit segment to be tested. The specific steps are as follows:

[0032] Open the corrected image using ImageJ software. Select the angle icon and measure 180° on the disk. Select Analyze, then set scale. Enter "180" for Known distance and "°" for Unit of length as a reference. In this example, four pins are used. Then measure the angle φ between the first and fourth pins on the fruit to be tested. Figure 1 (bottom right);

[0033] Use Excel to calculate the rotation degree of the middle section of the fruit:

[0034] RDM = φ / 3, unit is °cm -1 .

[0035] The present invention has been described in detail above. For those skilled in the art, the invention can be practiced in a wide range of ways with equivalent parameters, contents, and conditions without departing from its spirit and scope, and without requiring unnecessary experiments. Although specific embodiments have been given, it should be understood that further modifications can be made to the invention. In summary, according to the principles of the invention, this application is intended to include any changes, uses, or improvements to the invention, including changes made using conventional techniques known in the art that depart from the scope disclosed herein. Some basic features can be applied within the scope of the following appended claims.

Claims

1. A method for measuring the spiral degree of chili pepper fruits, characterized in that, Includes the following steps: S1. Cut a uniform spiral middle segment from the pepper fruit to be tested to obtain the fruit segment to be tested; S2. Insert a needle-like object perpendicular to the surface of the fruit on the spiral pattern of the fruit segment to be tested every 1 cm along the direction perpendicular to the cross-section of the fruit. Insert a total of n needles, where n is a natural number greater than or equal to 2. S3. Fix the fruit segment to be tested vertically on the testing platform with a black background, and place a reference object at the same time. Use a camera with the lens perpendicular to the testing platform to take a picture to obtain the original image. S4. Using computer image processing software, the lens distortion in the original image is corrected based on the reference object to obtain the corrected image; S5. Using computer image processing software, measure the angle φ between the 1st and nth needle-like objects in the corrected image, and calculate the helix angle RDM of the fruit segment to be tested: RDM = φ / (n-1), unit is °cm- 1 .

2. The method according to claim 1, characterized in that, Lens distortion is corrected using Photoshop software.

3. The method according to claim 1 or 2, characterized in that, The included angle φ was measured using ImageJ software.

4. The method according to any one of claims 1-3, characterized in that, The needle-like object is selected from pins, sewing needles, and toothpicks.

5. The method according to any one of claims 1-4, characterized in that, The length of the intermediate fruit segment is 3-6cm.

6. The method according to claim 5, characterized in that, The length of the middle fruit segment is 4-5cm.

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