Antimicrobial systems containing naturally derived ingredients and compositions containing them

Abstract

Described herein are antibacterial systems having naturally derived ingredients and compositions comprising the same. The systems have a first ingredient comprising thymol and a second natural ingredient comprising p-anisic acid, aloe vera, gluconolactone, tetrahydrocurcumin, 4-hydroxyacetophenone, or mixtures thereof.

Description

Technical Field

[0001] This invention relates to antimicrobial systems comprising naturally derived ingredients. More specifically, this invention relates to superior antimicrobial systems comprising thymol, which can be included in end-use formulations including cosmetic, personal care, and detergent compositions. In addition to thymol, the antimicrobial system of the present invention also comprises a second component, which is naturally derived and includes p-anesic acid, aloe vera, gluconolactone, tetrahydrocurcumin, 4-hydroxyacetophenone, or mixtures thereof. Such systems unexpectedly deliver excellent antimicrobial benefits when added to end-use compositions. Background Technology

[0002] DMDM hydantoin, parabens, methylisothiazolinones, and methylchloroisothiazolinones are commonly used preservatives in consumer products. These preservatives have been used for many years and are known to perform well in maintaining the integrity and stability of certain end-use compositions. However, there is a need to develop preservative systems containing naturally occurring components that are suitable for good performance across the entire range of consumer products. Naturally occurring preservative systems should be effective in preserving the product, not cause skin allergies, and not adversely affect the sensory properties of the consumer product, particularly those of topically applied products.

[0003] In addition to delivering excellent antibacterial benefits, this preservative system should not be harmful to the environment and is gentle enough to be used on even the most vulnerable consumer babies.

[0004] Therefore, the present invention relates to antimicrobial systems having naturally occurring components and compositions comprising such antimicrobial systems. In addition to a second naturally occurring component, which includes p-anesic acid, aloe vera, gluconolactone, tetrahydrocurcumin, 4-hydroxyacetophenone, or mixtures thereof, the preservative system also contains thymol as a first natural component. Such systems unexpectedly deliver excellent antimicrobial benefits to the end-use compositions in which they are used.

[0005] Additional Information

[0006] Efforts to prepare preservative systems have been disclosed. A preservative system for cosmetic formulations is described in U.S. Patent Application 2012 / 0190744A1.

[0007] Other efforts to prepare preservative systems have also been disclosed. U.S. Patent No. 7,754,774B2 describes a preservative-disinfectant containing 1,2-octanediol.

[0008] Other efforts to prepare preservative systems have even been disclosed. For example, U.S. Patent No. 7,582,681B2 describes an antimicrobial active complex having 1,2-alkyldiol.

[0009] None of the above additional information describes the antimicrobial systems as described and claimed in this application or the end-use compositions having such systems. Summary of the Invention

[0010] In a first aspect, the present invention relates to an antimicrobial system comprising:

[0011] (a) A first component, the first component comprising thymol; and

[0012] (b) A second component comprising p-anesic acid, aloe vera, gluconolactone, tetrahydrocurcumin, 4-hydroxyacetophenone, or a mixture thereof.

[0013] In a second aspect, the present invention relates to an end-use composition comprising:

[0014] (a) A first component, the first component comprising thymol; and

[0015] (b) A second component comprising p-anesic acid, aloe vera, gluconolactone, tetrahydrocurcumin, 4-hydroxyacetophenone, or a mixture thereof.

[0016] In a third aspect, the present invention relates to the use or method of preserving a composition having a first component and a second component, the first component comprising thymol and the second component comprising p-anesic acid, aloe, gluconolactone, tetrahydrocurcumin, 4-hydroxyacetophenone or a mixture thereof.

[0017] All other aspects of the invention will become more apparent from the following description and examples.

[0018] As used herein, "antimicrobial system" refers to a natural system of at least two components suitable for exhibiting synergistic antimicrobial benefits, including antimicrobial benefits as observed with preservatives. The at least two-component system refers to a system comprising thymol and a second component comprising anisolic acid, aloe vera, gluconolactone, tetrahydrocurcumin, 4-hydroxyacetophenone, or mixtures thereof, wherein the at least two-component system is an additive for end-use compositions (i.e., it can be sold as a separate additive composition for addition to end-use compositions, or formulated separately with these ingredients to prepare end-use compositions). Naturally derived means not requiring synthetic manufacturing and suitable for recovery from, for example, plants or roots (at least in precursor form). As used herein, "skin" includes skin on the feet, face, neck, chest, arms (including armpits), hands, legs, buttocks, back, and scalp (including hair). The end-use compositions described herein (i.e., compositions to be used or applied) include creams, lotions, serums, gels, balms, deodorants and antiperspirants, oral care compositions, shampoos, conditioners, bars and liquid detergents, and household care compositions such as hard surface and window cleaners, toilet cleaners, and laundry detergents. In one embodiment, the end-use composition of the present invention is a cleaning composition, washing product, or leave-on product, such as a cream or lotion applied to the face, body, or hands. In another embodiment, the end-use composition is a leave-on cosmetic product suitable for reducing the appearance of wrinkles and / or moisturizing the skin. Such compositions can also be compositions that cause the skin to have an even color or tone.

[0019] Unless otherwise expressly stated, all scopes described herein are to include all scopes included herein. The term “comprising” is to encompass the terms “consistently composed of” and “composed of”. For the avoidance of doubt, a composition comprising thymol and p-anesic acid means to include compositions consisting substantially of both substances and compositions consisting of both substances. Regarding percentages used herein, unless otherwise stated, the percentage means by weight. Except as expressly stated in the Examples and Comparative Examples, or where otherwise explicitly indicated, all numerical values ​​representing amounts, ratios of materials and / or their uses as used in this specification should be understood to be modified by “about”. Detailed Implementation

[0020] Besides preferably obtaining thymol in the most sustainable manner, there are no restrictions on how the source of thymol used in this invention is sought. Typically, pressurized liquid extraction or supercritical fluid extraction of thyme can be used to extract thymol. Other techniques include recovering thymol from the plant under ambient pressure and at a temperature of 30 to 45°C when extracted with limonene and ethanol. Alternatively, thyme oil and / or thyme extract containing thymol can be added to the antibacterial compositions of this invention, provided that the oil and extract contain thymol. Thyme oil and extract are obtained from the thyme plant. These plants belong to the genus *Thymus*, and include, but are not limited to, the following species: common thyme (*Thymus vulgaris*), white thyme (*Thymus zygis*), satureoides (*Thymus satureoides*), mastic thyme (*Thymus mastichina*), broussaneti (*Thymus broussaneti*), maroccamus (*Thymus maroccamus*), pale thyme (*Thymus pallidus*), Algerian thyme (*Thymus algeriensis*), European thyme (*Thymus serpyllum*), broadleaf thyme (*Thymus pulegoide*), and lemon thyme (*Thymus citrodorus*).

[0021] Typically, an end-use composition having a first component containing thymol will have 0.001% to 6% thymol. In one embodiment of the invention, the composition will have 0.01% to 4% thymol based on the total weight of the end-use composition, and in another embodiment, the composition will have 0.1% to 3% thymol. The extent to which thymol oil and / or extract is used in the end-use composition is such that the thymol level in the end-use composition is added in an amount that ensures the thymol level is consistent with the levels described herein.

[0022] As for the second component, which contains anisolic acid, aloe vera, gluconolactone, tetrahydrocurcumin, 4-hydroxyacetophenone, or mixtures thereof, these components are typically recovered from natural sources. Anisolic acid (4-methoxybenzoic acid) is naturally found in fennel, while aloe vera is recovered from the aloe vera plant. Gluconolactone is obtained from, for example, corn. Tetrahydrocurcumin is a metabolite of curcumin (found in turmeric), while 4-hydroxyacetophenone can be found in and recovered from tomatoes, cinnamon, and cocoa.

[0023] The second component of the antimicrobial system of the present invention typically accounts for 0.001% to 6% by weight of the end-use composition. In another embodiment, the second component accounts for 0.01% to 4.0% by weight of the end-use composition based on the total weight of the end-use composition, and in yet another embodiment, the second component accounts for 0.1% to 3.0% by weight of the end-use composition.

[0024] For an antimicrobial system having a first component comprising thymol and a second component comprising anisolic acid, aloe vera, gluconolactone, tetrahydrocurcumin, 4-hydroxyacetophenone, or a mixture thereof, the weight ratio of the first component to the second component in the antimicrobial system is typically 1:6 to 6:1. In another embodiment, the weight ratio of the first component to the second component is 4:1 to 1:4; in yet another embodiment, the weight ratio of the first component to the second component is 2:1 to 1:2, including all ratios included therein. The antimicrobial system may consist of thymol and the second component; however, an antimicrobial system comprising thymol and the second component, or substantially consisting of thymol and the second component, is also within the scope of the invention. In yet another embodiment of the invention, the antimicrobial system of the invention consists substantially of, or of, thymol and anisolic acid, aloe vera, gluconolactone, tetrahydrocurcumin, 4-hydroxyacetophenone, or a mixture thereof, wherein no additional antimicrobial component is required in the antimicrobial system. In another embodiment, the antimicrobial system may further comprise fragrance oil and / or fragrance (0.1% to 6% by weight of the antimicrobial system) and / or water (1% to 25% by weight of the antimicrobial system). In such an embodiment, the fragrance and / or fragrance oil are provided to deliver a desired odor or scent to the consumer.

[0025] There are generally no limitations on the type of end-use composition that may contain the antimicrobial system of the present invention. Suitable end-use compositions for use with the antimicrobial system typically contain a cosmetically acceptable carrier component. Water is the most preferred carrier. The amount of water may be from 1% to 96% by weight, preferably from 5% to 90% by weight, most preferably from 35% to 80% by weight, and most preferably from 40% to 75% by weight, based on the total weight of the end-use composition. Typically, the end-use composition of the present invention will be an emulsion of water and oil, most preferably of the oil-in-water type. However, water-in-oil emulsions, particularly those generally classified as water-in-oil emulsions with a high internal phase, are an option. Exemplary examples of high internal phase emulsions suitable for containing the antimicrobial system of the present invention are described in commonly owned U.S. Patent Application Publication No. 2008 / 0311058 and U.S. Patent No. 8,425,882, the disclosures of which are incorporated herein by reference.

[0026] Other cosmetically acceptable carriers suitable for use in this invention may include mineral oils, silicone oils, synthetic or natural esters, and alcohols. The amount of these materials may be from 0.1% to 50% by weight, preferably from 0.1% to 30% by weight, and most preferably from 1% to 20% by weight, including all ranges contained herein, based on the weight of the end-use composition. In yet another embodiment, such carriers comprise a total of 1% to 12% by weight of the end-use composition.

[0027] Silicone oils can be classified into volatile and non-volatile types. As used herein, the term "volatile" refers to those materials that have a measurable vapor pressure at ambient temperature. Volatile silicone oils are preferably selected from cyclic or linear polydimethylsiloxanes containing 3 to 9, preferably 4 to 5, silicon atoms.

[0028] Linear volatile polysiloxane materials typically have a viscosity of less than 5 centipoise at 25°C, while cyclic materials typically have a viscosity of less than 10 centipoise (measured at 20 RPM using a Brookfield viscometer with an RV 3 rotor, normalized to mineral oil).

[0029] Non-volatile silicone oils that can be used as carrier materials include polyalkylsiloxanes, polyalkylarylsiloxanes, and polyethersiloxane copolymers. The substantially non-volatile polyalkylsiloxanes available herein include, for example, polydimethylsiloxanes (e.g., dimethicone) having a viscosity of 5 to 100,000 centipoise at 25°C.

[0030] The preferred sources of siloxanes are usually cyclopentadioxane and polydimethylsiloxane alcohol solutions.

[0031] The suitable esters are:

[0032] (1) Alkenyl or alkyl esters of fatty acids having 10 to 20 carbon atoms, such as isopropyl palmitate, isopropyl isostearate, isononyl isononanoate, oleyl myristate, isopropyl myristate, oleyl stearate and oleyl oleate.

[0033] (2) Ether esters, such as fatty acid esters of ethoxylated fatty alcohols;

[0034] (3) Polyol esters, such as ethylene glycol mono-fatty acid esters and ethylene glycol di-fatty acid esters, diethylene glycol mono-fatty acid esters and diethylene glycol di-fatty acid esters, polyethylene glycol (200-6000) mono-fatty acid esters and polyethylene glycol (200-6000) di-fatty acid esters, propylene glycol mono-fatty acid esters and propylene glycol di-fatty acid esters, polypropylene glycol 2000 monooleate, polypropylene glycol 2000 monostearate, ethoxylated propylene glycol monostearate, glycerol mono-fatty acid esters and glycerol di-fatty acid esters, polyglycerol poly-fatty acid esters, ethoxylated glycerol monostearate, 1,3-butanediol monostearate, 1,3-butanediol distearate, polyoxyethylene polyol fatty acid esters, dehydrated sorbitol fatty acid esters and polyoxyethylene dehydrated sorbitol fatty acid esters;

[0035] (4) Wax esters, such as beeswax, cetyl alcohol, myristyl myristate, stearyl stearate; and

[0036] (5) Sterol esters, among which examples are soybean sterol fatty acid esters and cholesterol fatty acid esters.

[0037] Emulsifiers (or surfactants) may be present in the end-use composition comprising the antimicrobial system of the present invention. The total concentration of the emulsifier, based on the weight of the end-use composition, may be from 0.1% to 30% by weight, preferably from 2% to 20% by weight, and most preferably from 1% to 8% by weight. The emulsifier may be selected from anionic, nonionic, cationic, and amphoteric components. Particularly preferred nonionic components are those having C2 to 100 moles of ethylene oxide or propylene oxide condensed per mole of hydrophobic compound. 10 To C 20 Fatty alcohols or acidic hydrophobic compounds; C2-C condensed with 2 to 20 moles of olefin oxides. 10 Alkylphenols; mono- and di-fatty acid esters of ethylene glycol; fatty acid monoglycerides; dehydrated sorbitol mono-C8-C 20 Fatty acid esters and dehydrated sorbitol diC8-C 20 Fatty acid esters; and polyoxyethylene sorbitol; and combinations thereof. Alkyl polyglycosides and glycosidic fatty amides (e.g., methylglucosamides) are also suitable nonionic emulsifiers.

[0038] Preferred anionic emulsifiers include alkyl ether sulfates and alkyl ether sulfonates, alkyl sulfates and alkyl sulfonates, alkylbenzene sulfonates, alkyl sulfosuccinates and dialkyl sulfosuccinates, and C8-C. 20 Acyl hydroxyethanesulfonate (isethionate), C8-C 20Alkyl ether phosphates, alkyl ether carboxylates, and combinations thereof.

[0039] Cationic emulsifiers that can be used include, for example, palmitoylaminopropyltrimethylammonium chloride, distearate dimethylammonium chloride, and mixtures thereof. Amphoteric emulsifiers that can be used include cocoylaminopropyl betaine, C... 12 -C 20 Trialkyl betaine, sodium lauroyl amphotericate, sodium lauroyl diamphoacetate, or mixtures thereof.

[0040] Other commonly preferred emulsifiers include glyceryl stearate, diol stearate, stearamide AMP, PEG-100 stearate, cetyl alcohol, and emulsifying / thickening additives such as hydroxyethyl acrylate / sodium acryloyl dimethyl taurate copolymers / squalene and mixtures thereof.

[0041] Although not essential, conventional preservatives may optionally be added to end-use compositions comprising the antimicrobial system of the present invention to help resist the growth of potentially harmful microorganisms. Suitable conventional preservatives optionally used in the compositions of the present invention include alkyl esters of p-hydroxybenzoic acid. Other preservatives include derivatives of hydantoin, propionate salts, and various quaternary ammonium compounds. Generally preferred preservatives are sodium benzoate, iodopropynyl butylcarbamate, phenoxyethanol, methylparaben, propylparaben, imidazolidinyl urea, sodium dehydroacetate, and benzyl alcohol. Particularly preferred additives suitable for use with or without conventional preservatives optionally used in the present invention are 1,2-alkyldiols (e.g., 1,2-octanediol and 1,2-hexanediol).

[0042] Conventional fragrance components may be optionally added (either alone or together with the fragrance in the end-use composition), such as eugenol, coumarin, linalyl acetate, citronellol, iris concentrate, terpineyl acetate, pinene (α and β pinene) and citronellol.

[0043] If used, conventional preservatives, hydroxyl glycols, and / or fragrance components will account for no more than 2% by weight, preferably no more than 1% by weight, and most preferably no more than 0.6% by weight of the end-use composition of the present invention. In one embodiment of the present invention, 0.0001% to 0.85% by weight of optionally present preservatives, hydroxyl glycols, and / or fragrance components are used based on the total weight of the end-use composition. In a preferred embodiment of the present invention, conventional preservatives, hydroxyl glycols, and / or fragrance components (except for substances available in the fragrances used in the end-use composition) are not used in the end-use composition because such compositions contain the antimicrobial system of the present invention.

[0044] Thickeners may optionally be included in the end-use compositions of the present invention. Polysaccharides are particularly suitable. Examples include citrus fiber, starches, natural / synthetic gums, and celluloses. Representative starches are chemically modified starches, such as sodium hydroxypropyl starch phosphate and aluminum starch octenyl succinate. Tapioca starch is generally preferred. Suitable gums include xanthan gum, sclerotium gum, pectin, gum arabic, gum arabic, agar, guar gum, carrageenan, alginate, and combinations thereof. Suitable celluloses include hydroxypropyl cellulose, hydroxypropyl methylcellulose, ethyl cellulose, and sodium carboxymethyl cellulose. Synthetic polymers are another class of effective thickeners. This category includes crosslinked polyacrylates such as carbomers, and polyacrylamides such as... 305 and taurine copolymers such as Simulgel and AVC, the copolymers are identified by INCI nomenclature as sodium acrylate / sodium acryloyldimethyl taurate and acryloyldimethyl taurate / vinylpyrrolidone copolymers, respectively. Another preferred synthetic polymer suitable for thickening is an acrylic-based polymer that is commercially available from Seppic and sold under the name Simulgel IN100.

[0045] When used, the amount of the thickener may be from 0.001% to 5% by weight, preferably from 0.1% to 2% by weight, and most preferably from 0.2% to 0.5% by weight, based on the weight of the end-use composition.

[0046] Fragrances, fixatives, and abrasives may optionally be used in end-use compositions comprising the antimicrobial system of the present invention. The amount of each of these substances may be from 0.05% to 5% by weight, preferably from 0.1% to 3% by weight.

[0047] Conventional wetting agents can be used in the compositions of the present invention. These conventional wetting agents are typically polyol-type materials. Typical polyols include glycerol (i.e., glycerine or glycerin), propylene glycol, dipropylene glycol, polypropylene glycol, polyethylene glycol, sorbitol, hydroxypropyl sorbitol, hexanediol, 1,3-butanediol, isopentyl glycol, 1,2,6-hexanetriol, ethoxylated glycerol, propoxylated glycerol, and mixtures thereof. Glycerol, propylene glycol, or mixtures thereof are most preferred. The amount of wetting agent used may be from 0.5% to 20% by weight, preferably from 1% to 15% by weight, and most preferably from 2% to 10% by weight, based on the weight of the end-use composition.

[0048] The end-use compositions of the present invention may comprise vitamins. Exemplary vitamins are vitamin A (retinol) and retinol esters such as retinyl palmitate and retinyl propionate, vitamin B2, vitamin B3 (nicotinamide), vitamin B6, vitamin C, vitamin E, folic acid, and biotin. Derivatives of vitamins may also be used. For example, derivatives of vitamin C include ascorbate tetraisopalmitate, magnesium ascorbate phosphate, and ascorbate glycoside. Derivatives of vitamin E include tocopheryl acetate, tocopheryl palmitate, and tocopheryl linoleate. DL-panthenol and its derivatives may also be used. When present, the total amount of vitamins may be from 0.001% to 10% by weight, preferably from 0.01% to 5% by weight, and most preferably from 0.1% to 2% by weight, based on the weight of the end-use composition.

[0049] Other optional additives suitable for use in this invention include: resorcinols, such as 4-ethylresorcinol, 4-hexylresorcinol, 4-phenylethylresorcinol, dimethoxytolylpropylresorcinol, 4-cyclopentylresorcinol, 4-cyclohexylresorcinol; α- and / or β-hydroxy acids; phellandrene; conjugated linoleic acid; 12-hydroxystearic acid; and mixtures thereof. Other optional additives may also be included, such as ethanol, quaternary ammonium compounds (e.g., cetrimonium chloride, benzalkonium chloride, etc.), and lecithin. When used, these additives constitute a total of 0.001% to 12% by weight, preferably 0.01% to 6% by weight, and most preferably 0.1% to 4% by weight of the end-use composition.

[0050] Anti-dandruff promoters may be present. Examples include α-hydroxycarboxylic acids and β-hydroxycarboxylic acids. The term "acid" refers not only to free acids but also to their salts and C1-C2 compounds. 30 Alkyl or aryl esters, as well as lactones produced by the removal of water to form cyclic or linear lactone structures. Representative acids are glycolic acid and its derivatives, lactic acid, and malic acid. Salicylic acid is representative of β-hydroxycarboxylic acids. When present, these materials may be present in amounts from 0.01% to 15% by weight, preferably from 0.1% to 6% by weight, based on the weight of the composition for end use.

[0051] The end-use compositions of this invention may optionally contain a variety of herbal extracts. These extracts may be either water-soluble or water-insoluble, and are carried in solvents that are respectively hydrophilic or hydrophobic. Water and ethanol are preferred extraction solvents. Exemplary extracts include those removed from green tea, yarrow, chamomile, licorice, aloe vera, grape seed, Satsuma mandarin orange, willow bark, sage, and rosemary.

[0052] Also suitable alternatives include: chelating agents (e.g., EDTA), opacifying agents (e.g., TiO2, with a particle size of 50 nm to 1200 nm, preferably 50 nm to 350 nm), and C-treated materials. 8-22 Fatty acid-substituted sugars, lipoic acid, retinoxytrimethylsilane (available from Clariant Corp. under the trademark Silcare 1M-75), dehydroepiandrosterone (DHEA), and combinations thereof. Ceramides (including ceramide 1, ceramide 3, ceramide 3B, and ceramide 6) and pseudoceramides may also be optionally included. When using these materials, the amount of these materials may be from 0.0001% to 10% by weight, preferably from 0.001% to 6% by weight, and most preferably from 0.001% to 3% by weight, based on the weight of the end-use composition.

[0053] The sunscreen active agent may optionally be included in the composition for end use of the present invention. Particularly preferred are materials such as Parsol... Ethylhexyl p-methoxycinnamate is available commercially as Parsol. Avobenzene, and benzophenone-3, also known as hydroxybenzophenone. Inorganic sunscreen active agents, such as fine titanium dioxide, zinc oxide, polyethylene, and various other polymers, can be used. When present, the amount of the sunscreen agent is typically from 0.1% to 30% by weight, preferably from 0.5% to 20% by weight, and most preferably from 0.75% to 10% by weight.

[0054] Conventional buffers / pH adjusters can be used. These conventional buffers / pH adjusters include commonly used additives such as sodium hydroxide, potassium hydroxide, hydrochloric acid, citric acid, triethanolamine, citrate / citrate buffer, or mixtures thereof. In a particularly preferred embodiment, the composition of the present invention has a pH of 4 to 8, preferably 4.25 to 7.75, and most preferably 5.6 to 7.5.

[0055] In one embodiment of the invention, when the end-use composition is a conventional cleaning or washing composition, the composition typically contains 10% to 45% by weight of C8 to C96. 20 Preferably, it is C 10 To C 20 Or C 12 To C 18The cleaning or washing composition contains fatty acids or fatty acid soaps, and less than 5% by weight of a synthetic surfactant, preferably 1% to 3% by weight. In one embodiment of the invention, the cleaning or washing composition contains 0 to 5% by weight, preferably 0.01% to 4% by weight of 12-hydroxystearic acid (12-HSA) and 0.01% to 25% by weight of glycerol. In yet another embodiment, the end-use composition of the invention contains 0 to 6% by weight of a sulfate-based surfactant (e.g., sodium lauryl sulfate), but preferably does not contain (0.0% by weight) a sulfate-based surfactant.

[0056] The viscosity of the end-use compositions of this invention is typically from 1,000 cps to 50,000 cps. Washoff compositions preferably have a viscosity of less than 9,000 cps, while no-wash compositions preferably have a viscosity of 6,000 cps to 15,000 cps. Viscosity can be measured using instruments recognized in the art, such as the Brookfield viscometer RVT model D220, using a T-bar rotor D, at 5 RPM for 60 seconds at 25°C.

[0057] The following describes exemplary end-use compositions suitable for comprising 0.001 wt% to 6 wt% of a first component and 0.001 wt% to 6 wt% of a second component.

[0058] Body lotion for showering

[0059] Element weight% water margin Hydroxypropyl starch phosphate 2.0 Cetyl phosphate 1.0 potassium hydroxide 0.5 Hydroxypropyl dihydroxyethyl dimethyl ammonium chloride 3.0 Stearoylpropyl PG-dimethylammonium chloride phosphate 2.0 Butter 5.0 Coconut oil 5.5 Cocoyl octanoate 2.0 Cetearyl alcohol 1.5 essence 0.1

[0060] Body lotion

[0061] water margin Cetyl hydroxyethyl cellulose 0.25 Dehydrated sorbitan oleate decyl glucoside crosspolymer 3.5 potassium cetyl phosphate 1.0 glycerin 2.0 Ethylhexyl palmitate 3.0 Isopropyl palmitate 6.0 Avocado oil 3.0 cetyl alcohol 4.0 Diheptyl succinate and octyl glycerol / sebacic acid copolymer 2.0 essence 0.1

[0062] Shampoo composition

[0063]

[0064] When preparing these compositions, moderate heating (50°C to 60°C) and stirring at atmospheric pressure are used until a homogeneous composition is obtained. Homogenization is preferably included.

[0065] Various packaging options are available for storing and delivering end-use compositions containing the antimicrobial system of the present invention. Packaging typically depends on the type of product and its intended use. For example, leave-on skin lotions and creams, shampoos, conditioners, and shower gels are typically packaged in plastic containers with an opening at the dispensing end, which is covered by a closure. Metal cans pressurized by propellant and equipped with nozzles can be used as packaging for mousses and other personal care products.

[0066] The following embodiments are provided to illustrate the invention. These embodiments are not intended to limit the scope of the claims.

[0067] Example

[0068] Studies similar to those described below have demonstrated the unexpected synergistic benefits of using the natural antibacterial ingredients of this invention.

[0069] As shown in Table II, naturally derived antimicrobial systems were prepared by combining thymol with one of the following: anisolic acid, aloe vera, gluconolactone, tetrahydrocurcumin, and 4-hydroxyacetophenone. Two pools were established, each containing two replications. The strains involved were: *Pseudomonas aeruginosa*, *Pseudomonas putida*, *Burkholderia cepacia*, *Enterobacter gergoviae*, and *Klebsiella pneumoniae*. For all samples, the FIC test range (%) was 2.0–0.008. A 50 / 50 mixture of the natural component antimicrobial system was prepared by adding the first and second natural components (component A and component B).

[0070] The concepts of fractional concentration (FC) and fractional inhibitory concentration (FIC) have been used to extensively explore the different behaviors of antimicrobial agents in isolates and mixtures. See, for example, JRW Lambert and R Lambert, J. Appl. Microbiol 95, 734 (2003); T. Jadavji, CG Prober and R Cheung, Antibacterial Agents and Chemotherapy 26, 91 (1984); and WO 2004 / 006876. MIC is the minimum inhibitory concentration of a component that prevents the visible growth of one or more bacteria. FC and FIC parameters can be defined as follows:

[0071] FC (component A) = Concentration of component A tested in the mixture / MIC (component A tested as a single active ingredient).

[0072] FIC(component a) = MIC(component a tested in the mixture) / MIC(component a tested as a single active ingredient).

[0073] Interactions between antimicrobial agents can be cumulative, synergistic, or potentially antagonistic, depending on whether the combined potency is equal to, greater than, or less than the potency obtained by the individual components at the same total concentration when tested separately.

[0074] These relationships can be mathematically expressed by summing the fractional MIC values ​​of all components present in the mixture to give a "fractional inhibition index":

[0075] ∑FIC=FIC (组分1) +FIC (组分2)

[0076] Make:

[0077] A ∑FIC greater than 0.5 corresponds to indifferent, cumulative, or antagonistic activity.

[0078] A ∑FIC value less than or equal to 0.5 corresponds to synergistic activity.

[0079] A comparable method is the calculation of the synergy index (SI), an industrially acceptable method described by Kull, FC, Eisman, PC, Sylwestrowicz, HD, and Mayer, RL in Applied Microbiology 9:538-541 (1961).

[0080] Test methods

[0081] Liquid broth tests (MIC and checkerboard) were performed to identify one or more minimum concentrations of preservatives, both individually and in binary combinations. The revised method of ISO 20776-1:2006 was used for screening as follows. Stock solutions of preservatives and trypsin in soy broth were inoculated with 1×10⁻⁶ [amount missing]. 6 Up to 5×10 6 One microorganism was cultured at 30°C for 24 hours, and then the OD was measured. 600 Optical density at nm. The MIC was defined as a concentration at which <25% growth was observed compared to a positive growth control without preservatives. Preservatives were screened in the concentration range of 0.008 wt% to 2 wt%.

[0082] Table I: Microbial Pool

[0083]

[0084] Table II: Synergistic Combinations

[0085]

[0086] *Provides Water & Leuconostoc / Sorbusaucuparia Fruit Ferment Filtrate

[0087] The data provided in Table II unexpectedly show that when combined with natural antimicrobial ingredients conforming to the present invention, synergistic benefits against Gram-negative non-fermenting bacteria and Gram-negative fermenting bacteria are observed.

Claims

1. An antibacterial system, which consists of the following: (a) The first component, wherein the first component is thymol; and (b) A second component, wherein the second component is selected from p-anesic acid, gluconolactone, or tetrahydrocurcumin; The weight ratio of the first component to the second component is 1:6 to 6:

1.

2. A composition comprising the antimicrobial system according to claim 1, wherein, based on the total weight of the composition, the composition comprises 40% to 75% by weight of water, and wherein, if used, based on the total weight of the composition, the composition comprises no more than 0.6% by weight of a conventional preservative, a diol, and / or a fragrance component, wherein the conventional preservative is selected from sodium benzoate, iodopropynyl butylcarbamate, phenoxyethanol, methylparaben, propylparaben, imidazolidinyl urea, sodium dehydroacetate, and benzyl alcohol; the diol is selected from 1,2-octanediol and 1,2-hexanediol; and the fragrance component is selected from eugenol, coumarin, linalyl acetate, citronellol, iris concentrate, terpineol acetate, α- and β-pinene, and citronellol.

3. The composition of claim 2, wherein the composition comprises 0.001 wt% to 6 wt% of the first component and 0.001 wt% to 6 wt% of the second component.

4. The composition according to claim 2 or 3, wherein the composition is a bath liquid, shampoo, or topical skin composition.

5. The composition according to claim 2 or 3, wherein the composition further comprises salicylic acid, nicotinamide, 12-hydroxystearic acid, retinol, resorcinol, or mixtures thereof.

6. The composition according to claim 2 or 3, wherein the composition further comprises ceramide, sunscreen agent, glycerin, or mixtures thereof.

7. Use of the antimicrobial system according to claim 1, for preserving a composition comprising 40% to 75% by weight of water based on the total weight of the composition, and, if used, not more than 0.6% by weight of a conventional preservative, a diol, and / or a fragrance component based on the total weight of the composition, wherein the conventional preservative is selected from sodium benzoate, iodopropynyl butylcarbamate, phenoxyethanol, methylparaben, propylparaben, imidazolidinyl urea, sodium dehydroacetate, and benzyl alcohol; the diol is selected from 1,2-octanediol and 1,2-hexanediol; and the fragrance component is selected from eugenol, coumarin, linalyl acetate, citronellol, iris concentrate, terpineol acetate, α- and β-pinene, and citronellol.

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