模仿葡萄球菌HZ01在降解肌浆蛋白质中的应用
By using Staphylococcus aureus HZ01 as a starter culture, the disadvantage of commercial starter cultures in the face of competition from local microorganisms was solved, achieving efficient degradation of sarcoplasmic proteins and nitrates, and improving the flavor and quality of fermented meat products.
CN116875498BActive Publication Date: 2026-07-17ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
- Filing Date
- 2022-06-22
- Publication Date
- 2026-07-17
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Figure CN116875498B_ABST
Abstract
本发明涉及微生物食品发酵技术领域，具体公开了模仿葡萄球菌HZ01在降解肌浆蛋白质中的应用，包括以下步骤；提取肌浆蛋白质，并采用Lowry蛋白浓度试剂盒测定肌浆蛋白质的浓度；取肌浆蛋白质、葡萄糖和模仿葡萄球菌HZ01菌液共同孵育；待孵育完成后将菌液离心；取2×SDS上样缓冲液与离心后的上清液混合，并水浴；取10％‑12％的Bio‑Rad预制胶肌浆蛋白和标准蛋白，并在10％‑12％的Bio‑Rad预制胶肌浆蛋白和标准蛋白上分别上样品；上样后进行电泳；(S07)待电泳完成后用考马斯亮蓝R‑250进行染色，通过染色结果得出模仿葡萄球菌HZ01对肌浆蛋白质的降解结果。本发明具有加工适应性较好、蛋白酶和脂肪酶活性均较高、硝酸还原酶活性较好及发酵出的肉制品风味较佳的特点。
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