A sers immunoprobe for detecting zearalenone and a preparation method and application thereof

CN116908436BActive Publication Date: 2026-08-28SUZHOU UNIV
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Patent Information

Application Number
CN202310726789.8
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2023-06-19
Publication Date
2026-08-28
Estimated Expiration
2043-06-19

AI Technical Summary

Technical Problem

[0004]表面增强拉曼光谱(SERS)是己知灵敏度最高的一种分析方法，可达单分子检测水平，但SERS特异性较差；基于胶体金的侧流免疫层析法(LFIA)是一种操作简便的快速检测方法，应用广泛，但LFIA大多只能进行定性或半定量分析

Benefits of technology

[0036](1)本发明成功制备出SERS增强效果好的银包金立方纳米颗粒作为免疫探针的基底材料，并建立测定谷物及动物饲料中ZEN含量的表面增强拉曼光谱侧流免疫层析法。

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Abstract

The present application relates to a kind of detection of zearalenone SERS immunoprobe and its preparation method and application, food safety supervision or food analysis technical field.The present application discloses a kind of surface-enhanced Raman spectroscopy lateral flow immunochromatography (SERS-LFIA) for determining the content of zearalenone in grain and animal feed, two technologies are combined together with SERS and LFIA, silver-coated gold rod cubic nanoparticles are used as the base of immunoprobe, and the toxic and harmful substances in food can be realized with ultra-sensitivity, fast and strong specificity detection.The concentration range of standard curve is 0.14 pg / mL ‑1 ~100 ng / mL ‑1 , IC 50 50 pg / mL ‑1 The cross-reactivity with four structural analogues is 15.3%~31.2%, and there is almost no cross-reaction with other three mycotoxins;It is shown that the established SERS-LFIA not only has high sensitivity but also has strong specificity.
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Citation Information

Patent Citations

  • Surface-enhanced Raman scattering immunochromatography kit as well as preparation and application thereof

    CN114965428A

  • Nanocomposites, methods of making same, and applications of same for multicolor surface enhanced raman spectroscopy (SERS) detections

    US20160153975A1