A pearl polypeptide extract, polypeptide and application thereof in preparing tyrosinase inhibitor

Abstract

The application discloses a pearl polypeptide extract, polypeptides and application of the polypeptides in preparation of tyrosinase inhibitors, the extract is obtained by ultrasonic extraction of nano pearl powder with ultrapure water, dialysis, drying of the cut-off liquid, obtaining a crude product and loading SP-Sephadex G-50 filler column, combining the protein content greater than 0.05 mg / mL and the colored effluent, drying, obtaining the pearl polypeptide extract. The polypeptides are extracted from the nano pearl powder by the ultrapure water-ultrasonic method, the original structure of the polypeptides is better reserved, the use of acid volatile reagents is reduced, the equipment demand is low, the cycle is short, there is no pollution, and the process is simple. The small molecule polypeptides extracted by the application are more beneficial to skin absorption, the proportion of Mw less than 5000 Da is 93.28-96.45%, and the polypeptides have the effect of inhibiting tyrosinase activity, wherein the No. 95 polypeptide has significant tyrosinase inhibitory activity and small natural product side effects, and is suitable for subsequent pilot and industrial production.