Microfluidic chip for heterogeneous cell culture and monitoring and method of making the same
The application of microfluidic chip technology has solved the problem of insufficient assessment of tumor heterogeneity in traditional methods, enabling high-throughput culture and dynamic monitoring of heterogeneous tumor cells, guiding personalized treatment and drug screening, and improving the accuracy and predictive ability of tumor treatment.
CN116948823BActive Publication Date: 2026-07-14FUDAN UNIVERSITY
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- FUDAN UNIVERSITY
- Filing Date
- 2023-07-27
- Publication Date
- 2026-07-14
Smart Images
Figure CN116948823B_ABST
Abstract
The present application belongs to the technical field of microfluidic chip, and particularly relates to a microfluidic chip for heterogeneous cell culture and monitoring and a preparation method thereof. The microfluidic chip is composed of a polydimethylsiloxane (PDMS) substrate and a glass sheet, and a composite structure formed by combination of multiple functional units is designed on the PDMS substrate, and each functional unit is provided with a perfusion channel, a gel channel, a volcano-type capillary passive valve and a tumor cell channel. The microfluidic chip can be used for long-term and high-vigor culture of heterogeneous tumor cells in a three-dimensional in-vitro bionic environment, real-time dynamic observation of the interaction of multiple cells, monitoring of the differences between different subtypes of tumor cells in terms of functional characteristics, drug sensitivity, development speed and metastasis potential, and providing a novel high-throughput bionic platform for personalized treatment, tumor drug resistance research, disease progression prediction and new drug development.
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