A detection system and method for physiological metabolite release rate in the growth process of edible fungi

Abstract

The application discloses a kind of detection system and method of physiological metabolite release rate in the growth process of edible fungi, comprising: incubator, the two sides of the incubator are respectively provided with air inlet pipe and air outlet pipe, the side of the air inlet pipe is provided with air conditioning air supply device and the side of air outlet pipe is provided with pressure regulating air outlet, data processing module is connected outside the incubator.The data obtained by various sensors are substituted into the carbon dioxide release rate model, water vapor release rate model and heat release rate model in the growth process of edible fungi, respectively to obtain the carbon dioxide release rate, water vapor release rate and heat release rate in the growth process of edible fungi.

Description

Technical Field

[0001] This invention relates to the field of detection technology for the release rate of physiological metabolites during the growth process of edible fungi, and more specifically to a detection system and method for the release rate of physiological metabolites during the growth process of edible fungi. Background Technology

[0002] Metabolism is a constant physiological activity in the growth of organisms. Catabolism breaks down large molecules to obtain energy; anabolism uses energy to synthesize various components of the cell, such as proteins and nucleic acids. Metabolic processes include material metabolism and energy conversion. Most edible fungi are saprophytic fungi, which secrete various enzymes to hydrolyze high-molecular-weight cellulose and lignin in the culture medium into soluble low-molecular-weight monosaccharides, which are then absorbed and utilized to supply the growth and development of mycelia and fruiting bodies. Carbohydrates are one of the main energy sources in the life activities of edible fungi. They release a large amount of energy through a series of degradation processes within the fungus to meet the needs of life activities. The intermediate products produced during degradation can serve as carbon skeletons for the synthesis of proteins and fats, and pentose phosphate, obtained from the degradation of glucose via the pentose phosphate pathway, can be used for nucleic acid synthesis. Respiration plays a similar role in the energy metabolism of fungi as in other eukaryotes. The central structure of respiration is the mitochondria, and respiration includes three interdependent steps: the tricarboxylic acid cycle, electron transport, and oxidative phosphorylation. The tricarboxylic acid cycle, also known as the citric acid cycle or Krebs cycle, is the pivotal link between carbohydrate, lipid, and protein metabolism, and the pathway by which nutrients are ultimately oxidized in organisms. Each cycle of the tricarboxylic acid cycle releases a significant amount of chemical energy from metabolism, typically converted into high-energy phosphate compounds (ATP). In each phosphate group of ATP, the oxygen atom carries a negative charge due to its tendency to gain electrons, while the adjacent phosphorus atom carries a positive charge. During hydrolysis, ATP loses its terminal phosphate group to become adenosine diphosphate (ADP), releasing a large amount of energy. Other high-energy compounds also gradually release their stored energy, providing power for cellular physiological activities. The overall chemical reaction for the complete oxidation of pyruvate is: 2CH3COCOH + 5O2 → 6CO2 + 4H2O + free energy; this overall reaction represents the final result of energy metabolism in eukaryotes under aerobic respiration conditions.

[0003] Of the total free energy, most is used for the growth of edible fungi, with a portion released as heat. In summary, the final result of metabolism during the growth of edible fungi is the release of carbon dioxide (CO2), water vapor (H2O), and heat. The rates of carbon dioxide release, water vapor release, and heat release are used as physiological metabolic parameters for the growth process of edible fungi.

[0004] Carbon dioxide release rate: the mass of carbon dioxide released by the organism being tested per unit time;

[0005] Water vapor release rate: the mass of water vapor released by the tested organism per unit time;

[0006] Heat release rate: This is the amount of heat released by the organism being tested per unit time.

[0007] The above three parameters can reflect the physiological and metabolic state of edible fungi during their growth process.

[0008] Currently, there are many products available for detecting carbon dioxide, water vapor, and heat. However, in the field of edible mushroom cultivation, there is still no method or system for dynamically detecting the release rates of carbon dioxide, water vapor, and heat during mushroom growth. Therefore, a new technical solution is needed to address this issue. Summary of the Invention

[0009] The purpose of this invention is to provide a detection system and method for the release rate of physiological metabolites during the growth process of edible fungi, which solves the problem that there is no dynamic detection method and system for the release rate of carbon dioxide, water vapor and heat during the growth process of edible fungi in the field of edible fungi cultivation.

[0010] To achieve the above objectives, the present invention provides the following technical solution: a detection system and method for the release rate of physiological metabolites during the growth process of edible fungi, comprising: an incubator, wherein an air inlet pipe and an air outlet pipe are respectively provided on both sides of the incubator, an air conditioning air supply device is provided on the side of the air inlet pipe and a pressure regulating air outlet is provided on the side of the air outlet pipe, a data processing module is externally connected to the incubator, and the data interface of the data processing module is provided with an inlet humidity sensor, an inlet temperature sensor, an inlet carbon dioxide sensor, an inlet air volume detection device, an inlet relative humidity sensor, an inlet temperature sensor, an inlet carbon dioxide sensor, an outlet humidity sensor, an outlet temperature sensor and an outlet carbon dioxide sensor, wherein the inlet humidity sensor, the inlet temperature sensor, the inlet carbon dioxide sensor and the inlet air volume detection device are all installed inside the air inlet pipe, the inlet relative humidity sensor, the inlet temperature sensor and the inlet carbon dioxide sensor are all installed inside the incubator, and the outlet humidity sensor, the outlet temperature sensor and the outlet carbon dioxide sensor are all installed inside the outlet pipe.

[0011] In a preferred embodiment of the present invention, the control processing module includes a power supply control unit, a touch screen input display unit, a data acquisition unit, a data storage unit, a data processing unit, and a data communication unit;

[0012] The power supply control unit is used to control the air conditioning ventilation device and adjust the climate environment inside the incubator to meet the needs of edible fungi growth.

[0013] The touch screen input and display unit is used to input and set the detection sampling interval time, the volume of the incubator, the volume of the edible fungus culture medium, the atmospheric pressure, and to display the detection values ​​of various sensors and detection devices, as well as the values ​​of carbon dioxide release rate, water vapor release rate, and heat release rate.

[0014] The data acquisition unit is used to acquire real-time detection signals from the inlet relative humidity sensor, inlet temperature sensor, inlet carbon dioxide sensor, inlet air volume detection device, box relative humidity sensor, box temperature sensor, box carbon dioxide sensor, outlet relative humidity sensor, outlet temperature sensor, and outlet carbon dioxide sensor, and convert them into corresponding parameter values.

[0015] The data storage unit is used to store parameter values ​​and setting parameter values ​​for inlet relative humidity, inlet temperature, inlet carbon dioxide concentration, inlet air volume, chamber relative humidity, chamber temperature, chamber carbon dioxide concentration, outlet relative humidity, outlet temperature, and outlet carbon dioxide concentration, as well as the detected carbon dioxide release rate, water vapor release rate, and heat release rate.

[0016] The data processing unit calculates the carbon dioxide release rate, water vapor release rate, and heat release rate based on the sequentially stored data, including inlet relative humidity, inlet temperature, inlet carbon dioxide concentration, inlet air volume, chamber relative humidity, chamber temperature, chamber carbon dioxide concentration, outlet relative humidity, outlet temperature, outlet carbon dioxide concentration, carbon dioxide release rate model, water vapor release rate model, heat release rate model, and set parameters.

[0017] The data communication unit outputs real-time detection values ​​from various sensors and detection devices, as well as values ​​such as carbon dioxide release rate, water vapor release rate, and heat release rate, to the host computer and cloud platform via wired or wireless communication.

[0018] In a preferred embodiment of the present invention, the method for detecting the release rate of physiological metabolites during the growth process of edible fungi includes the following steps:

[0019] Step 1: Power on the control processing module and connect it to the air conditioning air supply device, the inlet air relative humidity sensor, the inlet air temperature sensor, the inlet air carbon dioxide sensor and the inlet air volume detection device, the box interior relative humidity sensor, the box interior air temperature sensor, the box interior carbon dioxide sensor, the outlet air relative humidity sensor, the outlet air temperature sensor and the outlet air carbon dioxide sensor. Check and confirm that all kinds of sensors and detection devices are communicating normally.

[0020] Step 2: Start and control the air conditioning unit according to the set control parameters through the control processing module, confirm that the air conditioning unit is operating normally, and adjust the climate environment in the incubator to a climate state that is suitable for the growth of edible fungi.

[0021] Step 3: Place the edible fungi culture medium to be tested in the incubator according to the process requirements, and then keep the incubator insulated and sealed.

[0022] Step 4: Input the detection sampling interval Δ through the data input display unit of the control processing module. t Incubator internal volume V n Volume V of edible fungi culture medium j Atmospheric pressure P;

[0023] Step 5: Initial data acquisition. The control and processing module acquires the detection signals from the relative humidity sensor, air temperature sensor, and carbon dioxide sensor inside the chamber through the data acquisition unit and converts them into relative humidity data. Temperature t inside the chamber n Carbon dioxide concentration C inside the chamber vn The data is stored in the data storage unit, and the sampling interval time is calculated.

[0024] Step 6: The control processing module collects detection signals from various sensors and detection devices through the data acquisition unit according to the set sampling interval, and converts the detection signals from the intake relative humidity sensor, intake temperature sensor, intake carbon dioxide sensor, and intake air volume detection device into intake relative humidity. Inlet air temperature t j_t Intake air carbon dioxide concentration C vj_t and intake air volume V j_t The detection signals from the relative humidity sensor, the air temperature sensor, and the carbon dioxide sensor inside the chamber are converted into the relative humidity inside the chamber. Temperature t inside the chamber n_t Carbon dioxide concentration C inside the chamber vn_t The detection signals from the outlet relative humidity sensor, outlet temperature sensor, and outlet carbon dioxide sensor are converted into outlet relative humidity. outlet air temperature t c_t Carbon dioxide concentration in the exhaust air (C) vc_t And store it in the data storage unit;

[0025] Step 7: The control processing module substitutes the corresponding parameter values ​​from the data storage unit into the carbon dioxide release rate model, water vapor release rate model, and heat release rate model, respectively, to calculate the carbon dioxide release rate S during the growth process of edible fungi. CO2 Water vapor release rate S H2O and heat release rate SHeat

[0026] Step 8: The control and processing module transmits various detection parameter values, carbon dioxide release rate, water vapor release rate, and heat release rate to the data storage unit, touch screen input and display unit, and data communication unit to realize data storage, display, and communication transmission;

[0027] Step 9: If the detection process is not finished, proceed to step 10; otherwise, proceed to step 11.

[0028] Step 10: The control processing module calculates the sampling interval time. If the sampling interval time has ended, proceed to step 6; otherwise, return to step 9.

[0029] Step 11: The detection process ends, the control processing module sends a shutdown command to the air conditioning air supply device, and the system enters standby mode.

[0030] In a preferred embodiment of the present invention, the carbon dioxide release rate model in step 7 is as follows:

[0031]

[0032] Wherein: S CO2_t This represents the current carbon dioxide emission rate;

[0033] V j_t This represents the current measured intake air volumetric flow rate;

[0034] t j_t This is the current measured inlet air temperature;

[0035] This represents the current measured relative humidity of the incoming air.

[0036] C vj_t This represents the current measured volumetric concentration of carbon dioxide in the incoming air.

[0037] P represents the local atmospheric pressure.

[0038] V n The volume of the incubator;

[0039] V j This refers to the volume of the edible fungi culture medium.

[0040] t n_t This is the current measured air temperature inside the incubator;

[0041] This represents the current measured relative humidity inside the incubator.

[0042] C vn_t This represents the current measured volume concentration of carbon dioxide in the incubator.

[0043] C vn_t为1 This refers to the volumetric concentration of carbon dioxide in the incubator as measured in the previous experiment.

[0044] C vc_t This represents the current measured volume concentration of carbon dioxide in the exhaust air.

[0045] In a preferred embodiment of the present invention, the water vapor release rate model in step 7 is as follows:

[0046]

[0047] in:

[0048] S H2O_t Current carbon dioxide emission rate

[0049] V j_t The current measured inlet volumetric flow rate

[0050] t j_t The current measured inlet air temperature

[0051] The current measured relative humidity of the incoming air

[0052] P represents the local atmospheric pressure.

[0053] V n For the incubator contents

[0054] V j For the volume of culture medium and scaffold

[0055] t n_t The current measured air temperature inside the incubator

[0056] The current measured relative humidity inside the incubator

[0057] The last time the relative humidity inside the incubator was measured

[0058] t c_t The current measured outlet air temperature

[0059] This represents the current measured relative humidity of the outlet air.

[0060] In a preferred embodiment of the present invention, the heat release rate model in step 7 is as follows:

[0061]

[0062] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0063] This invention places the edible fungus culture medium to be tested in an incubator. The air intake is controlled by an air conditioning system, and the air is introduced into the incubator through an intake duct. After mixing with the existing air in the incubator, the mixture is discharged through an outlet duct and a pressure-regulating outlet. The pressure-regulating outlet maintains a positive pressure within the incubator, thereby regulating the climate environment to meet the growth requirements of the edible fungi. The inlet relative humidity, temperature, carbon dioxide concentration, and airflow are detected by sensors for inlet relative humidity, temperature, and carbon dioxide concentration. Similarly, the incubator relative humidity, temperature, and carbon dioxide concentration are detected by sensors for incubator internal relative humidity, temperature, and carbon dioxide concentration. Finally, the outlet relative humidity, temperature, and carbon dioxide concentration are detected by sensors for outlet relative humidity, temperature, and carbon dioxide concentration. The control and processing module takes the corresponding values ​​of inlet relative humidity, inlet temperature, inlet carbon dioxide concentration, and inlet air volume; inlet relative humidity, inlet temperature, and inlet carbon dioxide concentration; outlet relative humidity, outlet temperature, and outlet carbon dioxide concentration; and the corresponding values ​​of sampling interval, incubator volume, edible fungus culture medium volume, and atmospheric pressure, and substitutes them into the carbon dioxide release rate model, water vapor release rate model, and heat release rate model for detecting the growth process of edible fungi, and calculates the carbon dioxide release rate, water vapor release rate, and heat release rate of the edible fungus growth process, respectively. Attached Figure Description

[0064] Figure 1 This is a schematic diagram of the detection system structure of the present invention.

[0065] In the diagram: 1. Air conditioning supply device; 2. Air inlet duct; 3. Data processing module; 4. Edible fungus culture medium; 5. Incubator; 6. Air outlet duct; 7. Air inlet humidity sensor; 8. Air inlet temperature sensor; 9. Air inlet carbon dioxide sensor; 10. Air inlet volume detection device; 11. Relative humidity sensor inside the chamber; 12. Temperature sensor inside the chamber; 13. Carbon dioxide sensor inside the chamber; 14. Pressure regulating air outlet; 15. Air outlet humidity sensor; 16. Air outlet temperature sensor; 17. Air outlet carbon dioxide sensor. Detailed Implementation

[0066] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0067] This invention provides a technical solution: a system and method for detecting the release rate of physiological metabolites during the growth process of edible fungi, comprising: an incubator 5, wherein an air inlet pipe 2 and an air outlet pipe 6 are respectively arranged on both sides of the incubator 5; an air conditioning air supply device 1 is arranged on the side of the air inlet pipe 2 and a pressure regulating air outlet 14 is arranged on the side of the air outlet pipe 6; a data processing module 3 is externally connected to the incubator 5; and the data interface of the data processing module 3 is provided with an air inlet humidity sensor 7, an air inlet temperature sensor 8, an air inlet carbon dioxide sensor 9, an air inlet volume detection device 10, and an internal relative humidity sensor. 11. The chamber includes an internal temperature sensor 12, an internal carbon dioxide sensor 13, an outlet humidity sensor 15, an outlet temperature sensor 16, and an outlet carbon dioxide sensor 17. The inlet humidity sensor 7, inlet temperature sensor 8, inlet carbon dioxide sensor 9, and inlet air volume detection device 10 are all installed inside the inlet pipe 2. The internal relative humidity sensor 11, internal temperature sensor 12, and internal carbon dioxide sensor 13 are all installed inside the incubator 5. The outlet humidity sensor 15, outlet temperature sensor 16, and outlet carbon dioxide sensor 17 are all installed inside the outlet pipe 6.

[0068] In a further improvement, the control processing module includes a power supply control unit, a touch screen input display unit, a data acquisition unit, a data storage unit, a data processing unit, and a data communication unit;

[0069] The power supply control unit is used to control the air conditioning ventilation device 1 and adjust the climate environment inside the incubator 5 to meet the needs of edible fungi growth.

[0070] The touch screen input display unit is used to input and set the detection sampling interval time, the internal volume of the incubator 5, the volume of the edible fungus culture medium 4, the atmospheric pressure, and to display the detection values ​​of various sensors and detection devices, as well as the values ​​of carbon dioxide release rate, water vapor release rate, and heat release rate.

[0071] The data acquisition unit is used to acquire real-time detection signals from the inlet relative humidity sensor, inlet temperature sensor 8, inlet carbon dioxide sensor 9, inlet air volume detection device 10, box interior relative humidity sensor 11, box interior temperature sensor 12, box interior carbon dioxide sensor 13, outlet relative humidity sensor, outlet temperature sensor 16, and outlet carbon dioxide sensor 17 and convert them into corresponding parameter values.

[0072] The data storage unit is used to store parameter values ​​and setting parameter values ​​for inlet relative humidity, inlet temperature, inlet carbon dioxide concentration, inlet air volume, chamber relative humidity, chamber temperature, chamber carbon dioxide concentration, outlet relative humidity, outlet temperature, and outlet carbon dioxide concentration, as well as the detected carbon dioxide release rate, water vapor release rate, and heat release rate.

[0073] The data processing unit calculates the carbon dioxide release rate, water vapor release rate, and heat release rate based on the sequentially stored data, including inlet relative humidity, inlet temperature, inlet carbon dioxide concentration, inlet air volume, chamber relative humidity, chamber temperature, chamber carbon dioxide concentration, outlet relative humidity, outlet temperature, outlet carbon dioxide concentration, carbon dioxide release rate model, water vapor release rate model, heat release rate model, and set parameters.

[0074] The data communication unit outputs real-time detection values ​​from various sensors and detection devices, as well as values ​​such as carbon dioxide release rate, water vapor release rate, and heat release rate, to the host computer and cloud platform via wired or wireless communication.

[0075] A further improved method for detecting the release rate of physiological metabolites during the growth process of edible fungi includes the following steps:

[0076] Step 1: Power on the control processing module and connect it to the air conditioning air supply device 1, the inlet air relative humidity sensor, the inlet air temperature sensor 8, the inlet air carbon dioxide sensor 9, the inlet air volume detection device 10, the box interior relative humidity sensor 11, the box interior air temperature sensor, the box interior carbon dioxide sensor 13, the outlet air relative humidity sensor, the outlet air temperature sensor 16, and the outlet air carbon dioxide sensor 17. Check and confirm that the communication of all kinds of sensors and detection devices is normal.

[0077] Step 2: Start and control the air conditioning air supply device 1 to run according to the set control parameters through the control processing module, confirm that the air conditioning air supply device 1 is running normally, and adjust the climate environment in the incubator 5 to a climate state that is suitable for the growth of edible fungi.

[0078] Step 3: Place the edible fungus culture medium 4 to be tested into the incubator 5 according to the process requirements, and then keep the incubator 5 insulated and sealed.

[0079] Step 4: Input the detection sampling interval time Δt and the internal volume V of the incubator 5 through the data input and display unit of the control processing module. n Volume V of edible fungus culture medium 4 j Atmospheric pressure P;

[0080] Step 5: Initial data acquisition. The control and processing module acquires the detection signals from the relative humidity sensor 11, the air temperature sensor, and the carbon dioxide sensor 13 inside the chamber through the data acquisition unit and converts them into relative humidity inside the chamber. Temperature t inside the chamber n Carbon dioxide concentration C inside the chamber vn The data is stored in the data storage unit, and the sampling interval time is calculated.

[0081] Step 6: The control processing module collects the detection signals from various sensors and detection devices through the data acquisition unit according to the set sampling interval time, and converts the detection signals from the intake relative humidity sensor, intake temperature sensor 8, intake carbon dioxide sensor 9, and intake air volume detection device 10 into intake relative humidity. Inlet air temperature t j_t Intake air carbon dioxide concentration C vj_t and intake air volume V j_t The detection signals from the relative humidity sensor 11, the air temperature sensor, and the carbon dioxide sensor 13 inside the chamber are converted into the relative humidity inside the chamber. Temperature t inside the chamber n_t Carbon dioxide concentration C inside the chamber vn_t The detection signals from the outlet relative humidity sensor, outlet temperature sensor 16, and outlet carbon dioxide sensor 17 are converted into outlet relative humidity. outlet air temperature t c_t Carbon dioxide concentration in the exhaust air (C) vc_t And store it in the data storage unit;

[0082] Step 7: The control processing module substitutes the corresponding parameter values ​​from the data storage unit into the carbon dioxide release rate model, water vapor release rate model, and heat release rate model, respectively, to calculate the carbon dioxide release rate S during the growth process of edible fungi. CO2 Water vapor release rate S H2O and heat release rate S Heat

[0083] Step 8: The control and processing module transmits various detection parameter values, carbon dioxide release rate, water vapor release rate, and heat release rate to the data storage unit, touch screen input and display unit, and data communication unit to realize data storage, display, and communication transmission;

[0084] Step 9: If the detection process is not finished, proceed to step 10; otherwise, proceed to step 11.

[0085] Step 10: The control processing module calculates the sampling interval time. If the sampling interval time has ended, proceed to step 6; otherwise, return to step 9.

[0086] Step 11: The detection process ends, the control processing module sends a shutdown command to the air conditioning air supply device 1, and the system enters standby mode.

[0087] A further improved version of the carbon dioxide release rate model in step 7 is:

[0088]

[0089] Wherein: S CO2_tThis represents the current carbon dioxide emission rate;

[0090] V j_t This represents the current measured intake air volumetric flow rate;

[0091] t j_t This is the current measured inlet air temperature;

[0092] This represents the current measured relative humidity of the incoming air.

[0093] C vj_t This represents the current measured volumetric concentration of carbon dioxide in the incoming air.

[0094] P represents the local atmospheric pressure.

[0095] V n The volume of the incubator;

[0096] V j This refers to the volume of the edible fungi culture medium.

[0097] t n_t This is the current measured air temperature inside the incubator;

[0098] This represents the current measured relative humidity inside the incubator.

[0099] C vn_t This represents the current measured volume concentration of carbon dioxide in the incubator.

[0100] C vn_t为1 This refers to the volumetric concentration of carbon dioxide in the incubator as measured in the previous experiment.

[0101] C vc_t This represents the current measured volume concentration of carbon dioxide in the exhaust air.

[0102] A further improved version of the water vapor release rate model in step 7 is:

[0103]

[0104] in:

[0105] S H2O_t Current carbon dioxide emission rate

[0106] V j_t The current measured inlet volumetric flow rate

[0107] t j_t The current measured inlet air temperature

[0108] The current measured relative humidity of the incoming air

[0109] P represents the local atmospheric pressure.

[0110] V n For the incubator contents

[0111] V j For the volume of culture medium and scaffold

[0112] t n_t The current measured air temperature inside the incubator

[0113] The current measured relative humidity inside the incubator

[0114] The relative humidity t in the incubator was measured in the last time. c_t The current measured outlet air temperature This represents the current measured relative humidity of the outlet air.

[0115] A further improved version of the heat release rate model in step 7 is:

[0116]

[0117] Working principle: The edible fungus culture medium 4 to be tested is placed in the incubator 5. The air intake is controlled by the air conditioning system 1 and sent into the incubator 5 through the air intake pipe 2. After mixing with the existing air in the incubator 5, the mixture is discharged from the incubator 5 through the air outlet pipe 6 and the pressure regulating outlet 14. The pressure regulating outlet 14 maintains a positive pressure state inside the incubator 5, thereby regulating the climate environment inside the incubator 5 to meet the needs of edible fungus growth. The relative humidity, temperature, carbon dioxide concentration, and air volume of the incoming air are detected by the relative humidity sensor, temperature sensor 8, carbon dioxide sensor 9, and air volume detection device 10. The relative humidity, temperature, and carbon dioxide concentration inside the chamber are detected by the relative humidity sensor 11, temperature sensor, and carbon dioxide sensor 13. The relative humidity, temperature, and carbon dioxide concentration of the outgoing air are detected by the relative humidity sensor, temperature sensor 16, and carbon dioxide sensor 17. The control and processing module takes the corresponding values ​​of inlet relative humidity, inlet temperature, inlet carbon dioxide concentration, and inlet air volume; inlet relative humidity, inlet temperature, and inlet carbon dioxide concentration; outlet relative humidity, outlet temperature, and outlet carbon dioxide concentration; and the corresponding values ​​of the sampling interval, incubator volume 5, edible fungus culture medium volume 4, and atmospheric pressure, and substitutes them into the carbon dioxide release rate model, water vapor release rate model, and heat release rate model for detecting the growth process of edible fungi, and calculates the carbon dioxide release rate, water vapor release rate, and heat release rate of the edible fungus growth process, respectively.

[0118] The foregoing has shown and described the basic principles, main features, and advantages of the present invention. It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that the invention can be implemented in other specific forms without departing from its spirit or essential characteristics. Therefore, the embodiments should be considered illustrative and non-limiting in all respects, and the scope of the invention is defined by the appended claims rather than the foregoing description. Thus, all variations falling within the meaning and scope of equivalents of the claims are intended to be included within the present invention. No reference numerals in the claims should be construed as limiting the scope of the claims.

[0119] Finally, the following points should be noted: First, in the description of this application, it should be noted that, unless otherwise specified and limited, the terms "installation", "connection", and "linkage" should be interpreted broadly, and can refer to mechanical or electrical connections, or internal connections between two components, or direct connections. "Up", "down", "left", "right", etc., are only used to indicate relative positional relationships. When the absolute position of the described object changes, the relative positional relationship may change.

[0120] Finally, it should be noted that the above descriptions are merely preferred embodiments of the present invention and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A system for detecting the release rate of physiological metabolites during the growth process of edible fungi, characterized in that: include: An incubator (5) is provided with an air inlet pipe (2) and an air outlet pipe (6) on both sides. An air conditioning air supply device (1) is provided on the side of the air inlet pipe (2) and a pressure regulating air outlet (14) is provided on the side of the air outlet pipe (6). A data processing module (3) is connected to the outside of the incubator (5). An air inlet humidity sensor (7), an air inlet temperature sensor (8), an air inlet carbon dioxide sensor (9), an air inlet volume detection device (10), an incubator relative humidity sensor (11), an incubator temperature sensor (12), and an incubator carbon dioxide sensor are provided at the data interface of the data processing module (3). The incubator (5) is equipped with an air inlet (6), an air outlet humidity sensor (13), an air outlet temperature sensor (15), an air outlet carbon dioxide sensor (16), and an air outlet carbon dioxide sensor (17). The air inlet humidity sensor (7), air inlet temperature sensor (8), air inlet carbon dioxide sensor (9), and air inlet volume detection device (10) are all installed in the air inlet pipe (2). The air inlet relative humidity sensor (11), air inlet temperature sensor (12), and air inlet carbon dioxide sensor (13) are all installed in the incubator (5). The air outlet humidity sensor (15), air outlet temperature sensor (16), and air outlet carbon dioxide sensor (17) are all installed in the air outlet pipe (6). The data processing module includes a power supply control unit, a touch screen input and display unit, a data acquisition unit, a data storage unit, a data processing unit, and a data communication unit; The power supply control unit is used to control the air conditioning air supply device (1) and adjust the climate environment inside the incubator (5) to meet the needs of edible fungi growth; The touch screen input display unit is used to input and set the detection sampling interval time, the internal volume of the incubator (5), the volume of the edible fungus culture medium (4), the atmospheric pressure, and to display the detection values ​​of various sensors and detection devices, as well as the values ​​of carbon dioxide release rate, water vapor release rate, and heat release rate. The data acquisition unit is used to acquire the real-time detection signals of the inlet relative humidity sensor, inlet temperature sensor (8), inlet carbon dioxide sensor (9), inlet air volume detection device (10), box relative humidity sensor (11), box temperature sensor (12), box carbon dioxide sensor (13), outlet relative humidity sensor, outlet temperature sensor (16), outlet carbon dioxide sensor (17) and convert them into corresponding parameter values. The data storage unit is used to store parameter values ​​and setting parameter values ​​for inlet relative humidity, inlet temperature, inlet carbon dioxide concentration, inlet air volume, chamber relative humidity, chamber temperature, chamber carbon dioxide concentration, outlet relative humidity, outlet temperature, and outlet carbon dioxide concentration, as well as the detected carbon dioxide release rate, water vapor release rate, and heat release rate. The data processing unit calculates the carbon dioxide release rate, water vapor release rate, and heat release rate based on the sequentially stored data, including inlet relative humidity, inlet temperature, inlet carbon dioxide concentration, inlet air volume, chamber relative humidity, chamber temperature, chamber carbon dioxide concentration, outlet relative humidity, outlet temperature, outlet carbon dioxide concentration, carbon dioxide release rate model, water vapor release rate model, heat release rate model, and set parameters. The data communication unit outputs real-time detection values ​​from various sensors and detection devices, as well as carbon dioxide release rate, water vapor release rate, and heat release rate values, to the host computer and cloud platform via wired or wireless communication.

2. A method for detecting the release rate of physiological metabolites during the growth process of edible fungi, characterized in that: The method for detecting the release rate of physiological metabolites during the growth process of edible fungi includes the following steps: Step 1: Power on the data processing module and connect it to the air conditioning supply device, inlet relative humidity sensor, inlet temperature sensor, inlet carbon dioxide sensor and inlet air volume detection device, box relative humidity sensor, box air temperature sensor, box carbon dioxide sensor, outlet relative humidity sensor, outlet temperature sensor and outlet carbon dioxide sensor. Check and confirm that all sensors and detection devices are communicating normally. Step 2: Start and control the air conditioning unit to run according to the set control parameters through the data processing module, confirm that the air conditioning unit is running normally, and adjust the climate environment in the incubator to a climate state that is suitable for the growth of edible fungi. Step 3: Place the edible fungi culture medium to be tested in the incubator according to the process requirements, and then keep the incubator insulated and sealed. Step 4: Input the detection sampling interval ∆ through the data input display unit of the data processing module. t Incubator internal volume V n Volume V of edible fungi culture medium j Atmospheric pressure P; Step 5: Initial data acquisition. The data processing module acquires the detection signals from the relative humidity sensor, air temperature sensor, and carbon dioxide sensor inside the chamber through the data acquisition unit and converts them into the relative humidity φ inside the chamber. n Temperature inside the chamber (t) n Carbon dioxide concentration C inside the chamber vn The data is stored in the data storage unit, and the sampling interval time is calculated. Step 6: The data processing module collects detection signals from various sensors and detection devices through the data acquisition unit according to the set sampling interval, and converts the detection signals from the intake relative humidity sensor, intake temperature sensor, intake carbon dioxide sensor, and intake air volume detection device into intake relative humidity φ. j_t Inlet air temperature t j_t , intake air carbon dioxide concentration C vj_t and intake air volume V j_t The detection signals from the relative humidity sensor, the air temperature sensor, and the carbon dioxide sensor inside the chamber are converted into the relative humidity φ inside the chamber. n_t Temperature inside the chamber (t) n_t Carbon dioxide concentration C inside the chamber vn_t The detection signals from the outlet relative humidity sensor, outlet temperature sensor, and outlet carbon dioxide sensor are converted into outlet relative humidity φ. c_t Air outlet temperature t c_t Carbon dioxide concentration in the exhaust air (C) vc_t， And store it in the data storage unit; Step 7: The data processing module substitutes the corresponding parameter values ​​from the data storage unit into the carbon dioxide release rate model, water vapor release rate model, and heat release rate model, respectively, to calculate the carbon dioxide release rate S during the growth process of edible fungi. CO2 Water vapor release rate S H2O and heat release rate S Heat Step 8: The data processing module transmits various detection parameter values, carbon dioxide release rate, water vapor release rate, and heat release rate to the data storage unit, touch screen input display unit, and data communication unit to realize data storage, display, and communication transmission; Step 9: If the detection process is not finished, proceed to step 10; otherwise, proceed to step 11. Step 10: The data processing module calculates the sampling interval time. If the sampling interval time has ended, proceed to step 6; otherwise, return to step 9. Step 11: The detection process ends, the data processing module sends a shutdown command to the air conditioning unit, and the system enters standby mode.

3. The method for detecting the release rate of physiological metabolites during the growth process of edible fungi according to claim 2, characterized in that: The carbon dioxide release rate model in step 7 is as follows: ; where: S CO2_t This represents the current carbon dioxide emission rate; V j_t This represents the current measured intake air volumetric flow rate; t j_t This is the current measured inlet air temperature; φ j_t This represents the current measured relative humidity of the incoming air. C vj_t This represents the current measured volumetric concentration of carbon dioxide in the incoming air. P represents the local atmospheric pressure. V n The volume of the incubator; V j This refers to the volume of the edible fungi culture medium. t n_t This is the current measured air temperature inside the incubator; φ n_t This represents the current measured relative humidity inside the incubator. C vn_t This represents the current measured volume concentration of carbon dioxide in the incubator. C vn_t为1 This refers to the volumetric concentration of carbon dioxide in the incubator as measured in the previous experiment. C vc_t This represents the current measured volume concentration of carbon dioxide in the exhaust air.

4. The method for detecting the release rate of physiological metabolites during the growth process of edible fungi according to claim 2, characterized in that: The water vapor release rate model in step 7 is: ； in: S H2O_t Current carbon dioxide emission rate V j_t The current measured inlet volumetric flow rate t j_t The current measured inlet air temperature φ j_t The current measured relative humidity of the incoming air P is used to measure local atmospheric pressure. V n For the incubator contents V j For the volume of culture medium and scaffold t n_t The current measured air temperature inside the incubator φ n_t The current measured relative humidity inside the incubator φ n_t为1 The last time the relative humidity inside the incubator was measured t c_t The current measured outlet air temperature φ c_t This represents the current measured relative humidity of the outlet air.

5. The method for detecting the release rate of physiological metabolites during the growth process of edible fungi according to claim 2, characterized in that: The heat release rate model in step 7 is as follows: 。

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