检测脱碱基核酸内切酶1的复合物荧光探针及其制备和应用
By designing a multifunctional protein-DNA complex fluorescent probe, an ACP complex is formed using a DNA probe modified with avidin and biotin-labeled AP site 3' double mismatch. This complex binds to phenylboronic acid-modified AVD and enters the cell nucleus, solving the sensitivity and specificity problems of APE1 detection and imaging in existing technologies. This enables efficient and accurate detection and imaging of APE1 in the nucleus of living cells.
CN116990266BActive Publication Date: 2026-07-17PEKING UNIV
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- PEKING UNIV
- Filing Date
- 2022-04-25
- Publication Date
- 2026-07-17
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Figure CN116990266B_ABST
Abstract
本发明公开了一种检测脱碱基核酸内切酶1的复合物荧光探针及其制备和应用。所述复合物荧光探针是亲和素与生物素标记的含错配修饰的DNA双链荧光探针形成的复合物，所述DNA双链荧光探针的探针链含缺碱基位点，其3’末端标记生物素，5’末端标记荧光基团，在缺碱基位点的3’方向第6个碱基处标记猝灭基团。该复合物荧光探针对碱基核酸内切酶1(APE1)有高灵敏度和特异性，可用于定量测定蛋白提取物中APE1的含量；进一步用苯硼酸修饰亲和素，所形成的复合物荧光探针通过脂质体转染的方法可进入活细胞的细胞细胞核，从而实现对细胞核内APE1的原位荧光成像。
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