Orchid fermentation liquor, preparation method and application thereof

Abstract

This invention provides an orchid fermentation broth, its preparation method, and its application, relating to the field of bio-fermentation technology. The preparation method of the orchid fermentation broth includes the following steps: Step 1, drying and pulverizing orchids to obtain orchid powder, and mixing the orchid powder with a culture medium to obtain a fermentation substrate; Step 2, inoculating the fermentation substrate with Bifidobacterium seed culture and carrying out anaerobic fermentation to obtain the orchid fermentation broth. This invention is the first to use Bifidobacterium to ferment Phalaenopsis orchids, obtaining a Phalaenopsis orchid fermentation broth. This fermentation broth is rich in various antioxidant active substances, total phenolic content, and β-glucan, exhibiting excellent antioxidant and anti-aging properties. This invention also provides a composition containing the above-mentioned Phalaenopsis orchid fermentation broth. This composition system is stable, does not easily change color when exposed to light, can be stored for a long time, and is safe and non-irritating.

Description

Technical Field

[0001] This application relates to the field of bio-fermentation technology, and in particular to an orchid fermentation broth, its preparation method, and its application. Background Technology

[0002] In recent years, orchid extracts prepared from orchid plants have been widely used in the cosmetics industry, including various orchid varieties such as Phalaenopsis, Oncidium, and Cymbidium. Studies have shown that the main skin-care and beauty active ingredients of orchid plants include polysaccharides, phenols, alkaloids, organosulfur compounds, and terpenoid volatile aromatic components, which have excellent skin-care and beauty effects such as moisturizing, anti-oxidation, anti-inflammation, whitening, anti-aging, antibacterial, and fragrance enhancement.

[0003] Phalaenopsis aphrodite Rchb.F., belonging to the genus Phalaenopsis in the family Orchidaceae, is native to subtropical rainforests and is an epiphytic orchid. Its large, white aerial roots protrude around the leaves, serving not only to absorb nutrients from the air but also for growth and photosynthesis. During the Lunar New Year, the Phalaenopsis plant sends forth long flower stalks from the leaf axils, producing flowers that resemble butterflies in flight, making it a favorite among flower enthusiasts and earning it the title of "Queen of Orchids." Currently, methods for preparing orchid extracts from Phalaenopsis are mostly based on alcohol extraction or ultrasonic extraction. These methods are relatively simple, but the concentration of active substances in the extracts still needs improvement. Furthermore, existing orchid extracts often exhibit problems such as discoloration upon exposure to light.

[0004] Bifida ferment lysate is a metabolite, cytoplasmic fragment, cell wall component, and polysaccharide complex obtained through the cultivation, inactivation, and decomposition of Bifidobacterium. It is rich in amino acids, vitamins, polypeptides, and minerals, and contains a variety of active ingredients. It has biological activities such as scavenging free radicals and inhibiting lipid peroxidation. It has a good effect on improving skin microecology and skin function, and has been widely used as a cosmetic raw material in the development of related products. Summary of the Invention

[0005] The purpose of this invention is to provide an orchid fermentation liquid, its preparation method, and its application. For the first time, Bifidobacterium is used to ferment Phalaenopsis orchids, resulting in a cosmetic raw material with both safety and antioxidant properties. This invention also provides a composition containing the orchid fermentation liquid. The composition system has good stability, is not easily decomposed by light, and can be stored for a long time.

[0006] On the one hand, this application provides a method for preparing orchid fermentation broth, the method comprising the following steps:

[0007] Step 1: Dry the orchids, pulverize them to obtain orchid powder, and mix the orchid powder with a culture medium to obtain a fermentation substrate;

[0008] Step 2: Inoculate the Bifidobacterium seed solution into the fermentation substrate and carry out anaerobic fermentation to obtain orchid fermentation broth; the Bifidobacterium is *Bifidobacterium longum* subsp. *longum* CICC24935; the OD of the Bifidobacterium seed solution... 600 The value is 5-6, and the inoculation amount of the Bifidobacterium seed solution is 1%-5%.

[0009] The Bifidobacterium mentioned was Bifidobacterium longum subsp. longum CICC24935, purchased from the China Industrial Microbial Culture Collection Center.

[0010] Preferably, the OD of the Bifidobacterium seed solution is... 600 The value was 5.5, which was verified to be the OD value of *Bifidobacterium longum* subsp. *longum* CICC24935. 600 When the value is 5.5, it reaches the logarithmic growth phase and the strain has high activity.

[0011] OD 600 This refers to the absorbance of the seed liquid at a wavelength of 600nm.

[0012] Preferably, the inoculation amount of the Bifidobacterium seed solution is 4%.

[0013] The method for culturing the Bifidobacterium seed culture is as follows: Bifidobacterium longum subsp. CICC24935 is inoculated into MRS solid medium at 37°C, 200 rpm, and pH 6.8, and cultured until OD... 600 The value is 5.5, and the culture time is approximately 24 hours.

[0014] Preferably, since the antioxidant active substances in orchid materials are mostly biological enzymes, and most biological enzymes are easily deactivated under high temperature conditions, the drying process is carried out at a low temperature of 20℃-50℃ in order to preserve the antioxidant components in orchid materials to the greatest extent.

[0015] Furthermore, the anaerobic fermentation conditions include: a temperature of 36.5℃-38.5℃, a rotation speed of 100-200 rpm, a pH value of 5-6, and a tank pressure of 3×10⁻⁶. 5 -4×10 5 Pa, time is 36-48h.

[0016] Preferably, the anaerobic fermentation conditions include: a temperature of 37.5°C, a rotation speed of 150 rpm, a pH value of 5, and a tank pressure of 3.530 × 10⁻⁶. 5 Pa, time is 36h.

[0017] In a preferred embodiment, the anaerobic fermentation process maintains an anaerobic environment by passing nitrogen gas at a 5% inoculum.

[0018] Furthermore, the orchid is a Phalaenopsis orchid; preferably, the orchid is a petal of a Phalaenopsis orchid in full bloom.

[0019] Furthermore, in step one, the orchid powder has a mesh size of 80-100; and / or,

[0020] The culture medium is MRS liquid culture medium; preferably, the mass ratio of orchid powder to culture medium is 1:(5-10).

[0021] Preferably, in step one, the orchid powder has a mesh size of 90; and / or,

[0022] The mass ratio of orchid powder to MRS liquid culture medium is 1:6.

[0023] In a preferred embodiment, the ratio of orchid powder to MRS liquid culture medium is 1g:6mL.

[0024] Furthermore, the preparation method further includes subjecting the orchid fermentation broth to high-pressure homogenization and crushing at 1200-1300 MPa, followed by filtration after crushing; preferably, the crushing temperature is not higher than 25°C; more preferably, the crushing temperature is 4°C-25°C.

[0025] Preferably, the pressure of the high-pressure homogenizer is 1300 MPa.

[0026] More preferably, the crushing process is performed in 6 cycles.

[0027] More preferably, the filtration is performed using a 50-220nm membrane to remove large molecular proteins and microorganisms, resulting in a sterile filtrate.

[0028] To preserve the antioxidant components in the orchid fermentation liquid to the greatest extent possible from being destroyed by high temperatures, the crushing process is carried out at a temperature of 4℃-25℃.

[0029] In a preferred embodiment, the method for preparing orchid fermentation broth includes the following steps:

[0030] Step 1: After cleaning the petals of the Phalaenopsis orchid during its blooming period, dry them at a low temperature of 20℃-50℃ and pulverize them to 80-100 mesh to obtain Phalaenopsis orchid powder. Mix the Phalaenopsis orchid powder and MRS liquid culture medium evenly at a ratio of 1g:5-10mL to obtain the fermentation substrate.

[0031] Step 2: Prepare Bifidobacterium seed culture (OD) 600The pH value is approximately 5-6. Inoculate 1%-5% of the substrate into a fermenter, maintaining a temperature of 36.5℃-38.5℃, a fermenter rotation speed of 100-200 rpm, and a pH of 5-6. During fermentation, maintain an anaerobic environment by circulating 4%-6% nitrogen gas through the inoculated solution. The fermenter pressure is 3 × 10⁻⁶. 5 -4×10 5 Pa, anaerobic fermentation time is 36-48h;

[0032] Step 3: After fermentation, the fermentation broth obtained is crushed using a high-pressure homogenizer. The homogenization pressure is 1200-1300 MPa, and the crushing process is repeated 3-6 times. During the crushing process, the temperature is controlled not to exceed 25℃. The homogenized broth after high-pressure homogenization is filtered through a 50-220 nm membrane to remove macromolecular proteins and microorganisms, resulting in a sterile filtrate. This filtrate is then stored in a low-temperature storage tank and used as the Phalaenopsis orchid fermentation broth.

[0033] On the other hand, this application provides an orchid fermentation broth prepared by the method described above.

[0034] On the other hand, this application provides a composition comprising the orchid fermentation broth as described above; preferably, the composition further comprises one or more of ectoine, hyaluronic acid, and tripeptide-1; more preferably, the molecular weight of the hyaluronic acid is 5000-10000 Da; even more preferably, by mass percentage, the composition comprises: 10%-20% orchid fermentation broth, 5%-10% ectoine, 5%-10% hyaluronic acid, 5%-10% tripeptide-1, with the balance being water.

[0035] More preferably, by weight percentage, the composition comprises: 15% Phalaenopsis orchid fermentation broth, 10% ectoine, 10% hyaluronic acid with a molecular weight of 5000-10000 Da, 5% tripeptide-1, and the balance being water.

[0036] On the other hand, this application also provides a cosmetic comprising the orchid fermentation liquid as described above or the composition as described above, wherein the dosage form of the cosmetic is selected from one of emulsion, aqueous solution, oil, and gel.

[0037] On the other hand, this application also provides the use of the orchid fermentation liquid as described above, or the composition as described above, or the cosmetic as described above in anti-oxidation, anti-aging and / or whitening.

[0038] The present invention has the following beneficial effects:

[0039] 1. This application is the first to use Bifidobacterium (Bifidobacterium longum subsp. CICC24935) to ferment Phalaenopsis orchids, and obtain Phalaenopsis orchid fermentation broth. The fermentation broth is rich in a variety of antioxidant active substances, and also contains Bifida Ferment Lysate (Bifidobacterium) lysate released after Bifidobacterium is broken up, as well as a variety of active substances produced by fermentation. It has high total phenol content and β-glucan content, and has excellent antioxidant properties.

[0040] 2. This application also provides a composition containing the above-mentioned Phalaenopsis orchid fermentation broth. The composition system is stable, does not easily change color when exposed to light, can be stored for a long time, and has excellent antioxidant and anti-aging properties. It is also safe and non-irritating, and can be widely used in the preparation and production of cosmetic products. Detailed Implementation

[0041] To more clearly illustrate the overall concept of this application, a detailed description is provided below by way of embodiments. Numerous specific details are set forth in the following description to provide a more thorough understanding of the invention. However, it will be apparent to those skilled in the art that the invention can be practiced without one or more of these details. In other instances, certain technical features well-known in the art have not been described to avoid confusion with the invention.

[0042] Unless otherwise specified in the examples, the conditions shall be performed in accordance with the standard conditions or the conditions recommended by the manufacturer.

[0043] MRS culture medium was purchased from Beijing Solarbio Science & Technology Co., Ltd.; Bifidobacterium longum subsp. longum CICC24935 and Bifidobacterium longum CICC6208 were purchased from China Industrial Microbial Culture Collection Center; Ectoin, hyaluronic acid, and tripeptide-1 were purchased from Wuhan Dopler Biopharmaceutical Co., Ltd.; Chamomile extract was purchased from Shanghai Zhuoding Biotechnology Co., Ltd.

[0044] Unless otherwise specified, in the following embodiments, reagents or instruments whose manufacturers are not indicated are all conventional products that can be purchased commercially.

[0045] MRS solid medium: 10.0g casein peptone, 10.0g beef extract, 5.0g yeast extract, 5.0g glucose, 5.0g sodium acetate, 2.0g diammonium citrate, 1.0g Tween 80, 2.0g K2HPO4, 0.2g MgSO4·7H2O, 0.05g MnSO4·H2O, 20.0g CaCO3, 15.0g agar, 1.0L distilled water, pH 6.8.

[0046] MRS liquid culture medium: 10.0g casein peptone, 10.0g beef extract, 5.0g yeast extract, 5.0g glucose, 5.0g sodium acetate, 2.0g diammonium citrate, 1.0g Tween 80, 2.0g K2HPO4, 0.2g MgSO4·7H2O, 0.05g MnSO4·H2O, 20.0g CaCO3, 1.0L distilled water, pH 6.8.

[0047] The Phalaenopsis orchids described below are specifically variegated Phalaenopsis orchids, purchased from the Institute of Leisure Agriculture, Shandong Academy of Agricultural Sciences.

[0048] Example 1: Relationship between Phalaenopsis orchid growth cycle and antioxidant capacity

[0049] Orchid extracts are widely used in the cosmetics industry, and various orchids have been proven to possess antioxidant capabilities. This application focuses on Phalaenopsis orchids to explore their specific antioxidant effects.

[0050] The specific experimental procedure included: cleaning Phalaenopsis orchid materials at different growth stages, weighing 0.1g of each material, adding 5mL of 20mmol / L KH2PO4, grinding into a homogenate in a mortar, centrifuging at 10000r / min for 10min, and collecting the supernatant. Superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) were used as representative indicators of antioxidant performance to test the supernatant. SOD activity was determined using the nitroblue tetrazolium photochemical reduction method, POD activity using the guaiacol colorimetric method, and CAT activity using the ultraviolet absorption method. The test results are shown in Table 1.

[0051] Table 1

[0052]

[0053] As shown in Table 1, the petals of Phalaenopsis orchids in their blooming and senescent stages contain higher levels of SOD, POD, and CAT, indicating that they have good antioxidant capacity.

[0054] Example 2: Phalaenopsis orchid fermentation experiment

[0055] Under the preferred conditions of Example 1, this application conducts fermentation experiments on Phalaenopsis orchids with different strains in order to obtain cosmetic materials with excellent properties.

[0056] The specific experimental procedure included: cleaning the Phalaenopsis orchid material, drying it at a low temperature of 20℃-50℃, pulverizing it to 80-100 mesh to obtain Phalaenopsis orchid powder, and mixing the Phalaenopsis orchid powder with MRS liquid culture medium at a material-to-liquid ratio of 1g:5-10mL to obtain the fermentation substrate. Different strains were used for anaerobic fermentation under different conditions. During fermentation, an anaerobic environment was maintained by passing nitrogen gas at an inoculum volume of 4%-6%, and the tank pressure was 3×10⁻⁶. 5 -4×10 5 After fermentation was completed, the SOD content in the fermentation broth was measured. The SOD activity was measured using the same method as in Example 1. The specific fermentation conditions and results are shown in Table 2. The total phenol content was determined using an industry-standard characteristic colorimetric method, and the β-glucan content was determined using the aniline blue colorimetric method.

[0057] Table 2

[0058]

[0059] As shown in Table 2, the petals of Phalaenopsis orchids in full bloom can produce more effective substances after fermentation with Bifidobacterium.

[0060] Fermentation of Phalaenopsis orchid petals during their blooming period using Bifidobacterium, especially Bifidobacterium longum subsp. CICC24935, helps to release superoxide dismutase (SOD) from the plant material, thereby increasing the SOD content in the fermentation broth. Furthermore, the fermentation process also produces more phenolic substances and polysaccharides (such as β-glucan). Previous studies have shown that phenolic substances in orchids are also related to antioxidant activity, while plant polysaccharides in orchids possess rich biological activities, giving them functions such as moisturizing, protecting against UV damage, treating acne, delaying aging, and whitening.

[0061] Example 3

[0062] Step 1: After cleaning the petals of the Phalaenopsis orchid during its blooming period, dry them at a low temperature of 20℃-50℃ and grind them to 90 mesh to obtain Phalaenopsis orchid powder. Mix the Phalaenopsis orchid powder and MRS liquid culture medium at a ratio of 1g:6mL to obtain the fermentation substrate.

[0063] Step 2: Prepare Bifidobacterium seed culture (OD) 600 The inoculum (approximately 5.5%) was inoculated into a fermenter containing the fermentation substrate at an inoculum size of 4%. The temperature was controlled at 37.5℃, the fermenter rotation speed at 150 rpm, and the pH was controlled at 5. During fermentation, an anaerobic environment was maintained by purging 5% nitrogen from the inoculum. The fermenter pressure was 3.530 × 10⁻⁶. 5 Pa, anaerobic fermentation time is 36h;

[0064] Step 3: The fermentation broth obtained after fermentation is completed is crushed using a high-pressure homogenizer. The homogenization pressure is 1300MPa, and the crushing process is repeated 6 times. During the crushing process, the temperature is controlled not to exceed 25℃. The homogenized broth after high-pressure homogenization is filtered through a 50-220nm membrane to remove macromolecular proteins and microorganisms, resulting in a sterile filtrate. This filtrate is then stored in a low-temperature storage tank and used as the Phalaenopsis orchid fermentation broth.

[0065] The Bifidobacterium mentioned was Bifidobacterium longum subsp. longum CICC24935, purchased from the China Industrial Microbial Culture Collection Center.

[0066] Cultivation method of Bifidobacterium seed culture: Inoculate Bifidobacterium longum subsp. CICC24935 into MRS solid medium at 37℃, 200 rpm, and pH 6.8 for approximately 24 hours, until OD reaches the target value. 600 The value is 5.5.

[0067] The Phalaenopsis orchid fermentation broth obtained in this embodiment is rich in antioxidant active substances, and also contains Bifida ferment lysate (Bifidobacterium) released after Bifidobacterium was broken up, as well as a variety of active substances produced by fermentation.

[0068] Example 4: Stability and Efficacy Tests

[0069] In this embodiment, the Phalaenopsis orchid fermentation broth obtained by the method in Example 3 was used as the sample for the experiment. During the experiment, it was found that although the Phalaenopsis orchid fermentation broth has excellent antioxidant activity, its stability is poor and it exhibits high sensitization during the test.

[0070] To improve the above problems, the Phalaenopsis orchid fermentation broth was compounded in this embodiment to obtain the following composition.

[0071] Composition 1: 15% Phalaenopsis orchid fermentation broth, 10% ectoine, 10% hyaluronic acid (5000-10000 da), 5% tripeptide-1, with the balance being water.

[0072] Composition 2: 15% Phalaenopsis orchid fermentation broth, 10% ectoine, 10% hyaluronic acid (10000-15000 Da), 5% tripeptide-1, with the balance being water.

[0073] Composition 3: 15% Phalaenopsis orchid fermentation broth, 10% ectoine, 5% tripeptide-1, with the balance being water.

[0074] Composition 4: 15% Phalaenopsis orchid fermentation broth, 10% ectoine, 10% hyaluronic acid (5000-10000 Da), with the balance being water.

[0075] Composition 5: Composition 1: 15% Phalaenopsis orchid fermentation broth, 10% hyaluronic acid (5000-10000 da), 5% tripeptide-1, with the balance being water.

[0076] Composition 6: Composition 1: 20% Phalaenopsis orchid fermentation broth, 10% Ectoin, 10% Hyaluronic acid (5000-10000Da), 5% Tripeptide-1, with the balance being water.

[0077] Composition 7: 15% Phalaenopsis orchid fermentation broth, 5% ectoine, 10% hyaluronic acid (5000-10000 Da), 5% tripeptide-1, with the balance being water.

[0078] Composition 8: 15% Phalaenopsis orchid fermentation broth, 10% ectoine, 10% hyaluronic acid (5000-10000 Da), 5% chamomile extract, and the balance being water.

[0079] Comparative Example: 15% orchid extract, 5% bifida ferment lysate, 10% ectoine, 10% hyaluronic acid (5000-10000 Da), 5% tripeptide-1, balance water.

[0080] Blank control group: 100% Phalaenopsis orchid fermentation broth.

[0081] Stability test

[0082] In this embodiment, it was found that the Phalaenopsis orchid fermentation broth had poor stability, specifically manifested in the easy discoloration and precipitation of the fermentation broth after exposure to light. Therefore, in this embodiment, the above-mentioned composition was used to conduct a stability experiment. Each composition was subjected to 8 hours of light treatment per day for one week. After one week, the stability of each composition was observed, and the specific observation results are shown in Table 3.

[0083] Table 3

[0084]

[0085] As shown in Table 3, hyaluronic acid with a specific molecular weight helps increase the stability of the composition system and makes it less prone to discoloration upon exposure to light. Composition 1 in this example exhibited good system stability, did not easily discolor upon exposure to light, and did not show any layering. It can be stored in sunlight for a long time, which is beneficial for product manufacturing.

[0086] Efficacy test

[0087] In this embodiment, the above-mentioned composition was used to conduct efficacy and performance experiments, specifically including:

[0088] In this embodiment, the above composition was used as the test substance for patch testing. Thirty subjects who met the subject voluntary inclusion criteria were selected as subjects, including 15 males and 15 females, aged 20 to 59 years, with an average age of 45.32 ± 1.03 years.

[0089] The specific testing method involves using qualified patch testing equipment and employing a closed patch test method. Approximately 0.020g-0.025g of the test substance is placed inside the patch testing device, and a hypoallergenic adhesive tape is applied to the inner forearm of the subject. The test substance is removed after 24 hours, and the skin reaction is observed 0.5 hours after removal. The results are recorded according to the skin reaction grading standards in the currently effective technical specifications. The specific standards are shown in Table 4, and the results are shown in Table 5.

[0090] The current effective technical specifications for skin reaction grading standards are: Chapter 7, Section 2 of the "Cosmetic Safety Technical Specifications" (2015 Edition) on human patch testing (China Food and Drug Administration).

[0091] Table 4. Grading Criteria for Skin Reactions in Closed Patch Tests

[0092]

[0093] Table 5 Summary of Cosmetic Skin Patch Test Results

[0094]

[0095] As shown in Table 5, when Composition 1 was used as the test substance, none of the 30 subjects had any adverse reactions, proving that Composition 1 provided in this application is safe and non-irritating.

[0096] In this embodiment, the content of SOD, POD and CAT in the above composition was determined by the method in Example 1. Each group was measured three times, and the average value of the measurement results was taken. The measurement results are shown in Table 5.

[0097] Table 5

[0098]

[0099] As can be seen from the results in Table 5, the composition 1 of this embodiment contains more antioxidant components and has superior antioxidant properties compared to existing products.

[0100] In this embodiment, an in vitro premature aging model of human skin fibroblasts induced by ultraviolet light was also used, and the content of matrix metalloproteinase-1 (MMP-1) in the extracellular matrix was detected to evaluate the anti-aging effect of composition 1. The results are shown in Table 6.

[0101] Table 6

[0102]

[0103] As shown in Table 6, this embodiment demonstrates that composition 1 has excellent anti-aging effects.

[0104] The above description is merely an embodiment of this application and is not intended to limit the scope of this application. Various modifications and variations can be made to this application by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this application should be included within the scope of the claims of this application.

Claims

1. An antioxidant, anti-aging and / or whitening composition characterized in that, The composition, by weight percentage, comprises: 15% orchid fermentation broth, 10% ectoine, 10% hyaluronic acid with a molecular weight of 5000-10000 Da, 5% tripeptide-1, and the balance being water. The method for preparing the orchid fermentation broth includes the following steps: Step 1: Dry and pulverize the orchid to obtain orchid powder. Mix the orchid powder with a culture medium to obtain a fermentation substrate. The orchid is a petal of a fully blooming Phalaenopsis orchid. The drying process is carried out at a low temperature of 20℃-50℃. The orchid powder has a mesh size of 80-100 mesh. The culture medium is MRS liquid medium. The mass ratio of the orchid powder to the MRS liquid medium is 1:(5-10). Step 2: Inoculate the Bifidobacterium seed culture into the fermentation substrate and carry out anaerobic fermentation to obtain orchid fermentation broth; the Bifidobacterium is *Bifidobacterium longum* subsp. *longum* (… Bifidobacterium longum subsp. longum CICC24935; the OD of the Bifidobacterium seed solution 600 The pH value is 5-6, and the inoculum size of the Bifidobacterium seed solution is 1%-5%; the anaerobic fermentation conditions are: temperature 36.5℃-38.5℃, rotation speed 100-200 rpm, pH value 5-6, and tank pressure 3×10⁻⁶. 5 -4×10 5 Pa, time is 36-48 h; The preparation method further includes subjecting the orchid fermentation broth to high-pressure homogenization and crushing at 1200-1300 MPa, followed by filtration after crushing. The crushing temperature is 4℃-25℃. The crushing process is repeated 6 times. The filtration is performed using a 50-220 nm membrane.

2. A cosmetic comprising the composition as described in claim 1, characterized in that, The dosage form of the cosmetic is selected from one of the following: emulsion, aqueous solution, oil, and gel.

3. The use of the composition of claim 1 or the cosmetic of claim 2 in the preparation of antioxidant, anti-aging and / or whitening products.

Images