棘孢曲霉、含有该菌的菌剂及它们的应用以及转化黄芪甲苷的方法

The microbial transformation method using Aspergillus echinospora XXLS-Q1 strain solved the production problem of cycloastragaloside-6-O-β-D-glucoside, achieving efficient conversion of astragaloside A to cycloastragaloside-6-O-β-D-xyloside, simplifying the operation and reducing environmental pollution.

CN117025410BActive Publication Date: 2026-07-17NANJING NORMAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
NANJING NORMAL UNIVERSITY
Filing Date
2023-07-28
Publication Date
2026-07-17

AI Technical Summary

Technical Problem

In existing technologies, the production of cycloastragaloyl-6-O-β-D-glucoside is difficult to operate, pollutes a lot, and consumes a lot of energy. In addition, traditional hydrolysis chemical methods are costly and have low yields, making it difficult to efficiently convert astragaloyl A into cycloastragaloyl-6-O-β-D-xyloside.

Method used

Microbial transformation was carried out using Aspergillus aculeatus strain XXLS-Q1. Through fermentation culture under specific temperature and pH conditions, β-glucosidase was produced to convert astragaloside A into cycloastragalool-6-O-β-D-xyloside, avoiding the use of strong oxidants and strong acids and bases, thus reducing environmental pollution.

Benefits of technology

The efficient conversion of astragaloside A to cycloastragaloyl-6-O-β-D-xyloside was achieved. The operation is simple, the reaction conditions are mild, environmental pollution is reduced, and the conversion rate and production efficiency are improved.

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Abstract

本发明涉及微生物技术领域，公开了一种棘孢曲霉、含有该菌的菌剂及它们的应用以及转化黄芪甲苷的方法。所述棘孢曲霉的菌株名为棘孢曲霉XXLS‑Q1(Aspergillus aculeatus XXLS‑Q1)，保藏编号为CCTCC NO:M20221645。本发明提供的菌剂含有上述的棘孢曲霉。本发明还提供了上述棘孢曲霉、上述的菌株在制备β‑葡萄糖苷酶和 / 或转化黄芪甲苷中的应用。转化黄芪甲苷的方法包括以下步骤：将上述的棘孢曲霉和 / 或上述的菌剂与含黄芪甲苷的原料接触。本发明提供的棘孢曲霉可实现黄芪甲苷到环黄芪醇‑6‑O‑β‑D‑木糖苷的高效转化，且操作简单、反应条件温和，有效减少对环境的污染。
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Citation Information

Patent Citations

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