改进的CG碱基编辑系统
By using a combination of cytosine deaminase, nuclease-inactivated CRISPR effector protein, and uracil-DNA glycosylase in plants, along with guide RNA and peptide fusions, the C-to-G base editing system was optimized, solving the problems of low efficiency and numerous Indel mutations in existing technologies, and achieving efficient and precise C-to-G base substitution.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- SUZHOU QI BIODESIGN BIOTECHNOLOGY CO LTD
- Filing Date
- 2022-03-09
- Publication Date
- 2026-07-17
AI Technical Summary
Existing gene editing technologies struggle to achieve efficient and precise C-to-G base substitution in plants and are prone to Indel mutations, resulting in the immaturity of the development and optimization of plant CG base editing systems.
A C-to-G base editing system is employed, comprising a combination of cytosine deaminase, nuclease-inactivated CRISPR effector protein, and uracil-DNA glycosylase. This system, combined with guide RNA, targets and edits the cellular genome. The editing process is further optimized by using a fusion of proliferating cell nuclear antigen and ubiquitin protein with mutated ubiquitin binding sites, as well as peptides such as mutated AP endonuclease.
This technology enables efficient and precise C-to-G base substitution in plant genomes, reducing the generation of Indel mutations, expanding the scope of single-base editing, and improving editing efficiency and purity.
Smart Images
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