Preparation method of fish skin collagen peptide with high ACE inhibitory activity

CN117089591BActive Publication Date: 2026-08-28JIANGNAN UNIV
View PDF 4 Cites 0 Cited by

Patent Information

Application Number
CN202311154190.8
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2023-09-07
Publication Date
2026-08-28
Estimated Expiration
2043-09-07

AI Technical Summary

Technical Problem

[0006]常规制备ACE抑制肽的方法存在的复合酶使用量大、产品分子量偏高、产品中盐和游离氨基酸残留以及功能活性和特异性肽段没有明确对应关系、ACE抑制率低的问题

Benefits of technology

[0033](1)本发明采用超声预处理可以破坏胶原蛋白的非共价键，改变其二级结构和官能团，有利于酶渗透到样品基质中，提升酶解效率；同时超声处理可以使得蛋白质构象变化，形成更多的β螺旋和β转角并且破坏二硫键，改善胶原蛋白肽的ACE抑制活性。

✦ Generated by Eureka AI based on patent content.

Smart Images

  • Figure CN117089591B_ABST
    Figure CN117089591B_ABST
Patent Text Reader

Abstract

The application discloses a preparation method of fish skin collagen peptide with high ACE inhibitory activity, and belongs to the technical field of food. The preparation method of fish skin collagen peptide with high ACE inhibitory activity comprises the following steps: (1) swelling fish skin gelatin; (2) performing ultrasonic treatment on the swollen fish skin gelatin; (3) performing enzymolysis on the ultrasonic-treated fish skin gelatin by using papain to obtain fish skin gelatin hydrolysate of first-step enzymolysis; (4) performing enzymolysis on the fish skin gelatin hydrolysate of first-step enzymolysis by using ginger protease to obtain fish skin gelatin hydrolysate of second-step enzymolysis; and (5) performing filtration, sterilization and freeze-drying on the fish skin gelatin hydrolysate of second-step enzymolysis to obtain fish skin collagen peptide with high ACE inhibitory activity. The method can effectively improve the ACE inhibitory activity of collagen peptide, and can minimize the use amount of compound enzyme and the residues of salt and free amino acid in the product.
Need to check novelty before this filing date? Find Prior Art

Citation Information

Patent Citations

  • A method for preparing fish skin collagen and ACE inhibitory peptides

    CN103923963B

  • Flatfish skin collagen-sourced ACE inhibiting peptide and preparation method thereof

    CN109232731A

  • Collagen peptide composition production method, DPP-4 inhibitor, and antihyperglycemic agent

    CN104540960A

  • Preparation method of high-purity fish skin collagen tripeptide

    CN116162677A