Thermal imaging-based online observation method and device for microbial nocturnal activity state

Abstract

The application provides a kind of microorganism night activity state online observation method and device based on thermal imager, and the observation method can greatly improve the definition of thermal image by constructing a new mask operator.The device includes a movable support base, an up-down adjustment module and a rotary adjustment module, a deblurring algorithm based on a four-leaf vector method, and an upper computer connected by a data line.The three-dimensional adjustment of the online observation device is used to obtain thermal image data at different positions, different heights and different angles, and then the thermal image data is processed based on the deblurring algorithm of the four-leaf vector method, and the computer displays the freshwater microorganism night activity state.The application can observe the freshwater microorganism night activity state online at night, monitor the activity state of photosensitive plankton, provide data support for fully revealing the lake eutrophication level and water quality change, and has simple structure and fast measurement speed.

Description

Technical Field

[0001] This invention relates to the field of observation devices, specifically to a method and device for online observation of the nighttime activity status of microorganisms based on a thermal imager. Background Technology

[0002] Phytoplankton, mainly composed of phytoplankton and zooplankton, are an important component of aquatic life in lakes and reservoirs, playing a crucial role in energy flow, material cycling, and information transmission within aquatic ecosystems. On the one hand, as primary producers in aquatic ecosystems, phytoplankton plays a vital role; on the other hand, due to their sensitive and rapid response to changes in environmental factors, phytoplankton can serve as an "indicator" of water quality changes and a basis for water quality assessment. Monitoring the community composition and changes of phytoplankton is crucial for revealing the eutrophication level and water quality changes in lakes. However, some phytoplankton are photosensitive and only active at night; therefore, observing phytoplankton activity at night is of great significance. However, due to limitations such as nighttime light, the acquired images are often of poor clarity, hindering the observation of freshwater microbial activity at night. Furthermore, existing image capturing devices are generally fixed in position, making it difficult to quickly adjust to the optimal location for capturing better thermal images. Therefore, there is an urgent need to research a new online observation device and method to obtain higher-quality thermal images for better observation of freshwater microbial activity at night. Summary of the Invention

[0003] To address the shortcomings of the existing technologies, the present invention aims to provide an online observation method and device for the nighttime activity of microorganisms based on a thermal imager. This method not only meets the requirements of complex nighttime environments but also enables online observation of the nighttime activity of microorganisms in freshwater areas at different locations and angles. Furthermore, it eliminates the blurring effect of infrared thermal images caused by plankton movement, thereby enabling the understanding of the community composition and changes of plankton and providing data support for revealing the eutrophication level and water quality changes in lakes.

[0004] Specifically, the present invention provides an online observation method for the nighttime activity status of microorganisms based on a thermal imager, which includes the following steps:

[0005] S1. Move the observation device to the location for online observation and adjust the height and angle of the thermal imager, specifically including the following sub-steps:

[0006] S11. Move the observation device to a horizontal position for online observation using the low center of gravity adjustment wheels of the movable support base;

[0007] S12. Adjust the height of the thermal imager by moving the movable guide rail connecting plate on the up-down adjustment module, and fix the height position by the self-locking device of the movable guide rail connecting plate.

[0008] S13. The guide rod rotating device of the rotating module drives the thermal imager rotating fixed base to rotate, thereby driving the thermal imager to rotate and adjusting the angle of the thermal imager;

[0009] S2. Power on the thermal imager and connect it to the host computer. The thermal imager acquires thermal images of microbial activity at night and uploads them to the host computer.

[0010] S3. During the observation process, the height of the thermal imager is changed by the up-down adjustment device and / or the angle of the thermal imager is changed by the rotation module to obtain multiple original thermal images of microbial nighttime activity.

[0011] S4. The original thermal image of nighttime microbial activity is processed using the thermal image processing unit inside the host computer to obtain a clear image, which includes the following sub-steps:

[0012] S41. Based on the original thermal images of microbial nighttime activity, a new mask operator is constructed using the four-leaf vector method. i,j This represents the thermal image data point at position (i,j); it will be compared with A i,j Eight adjacent thermal image data points are divided into four regions, and the two thermal image data points in each region are related to A. i,j They form a plane, denoted as s1, s2, s3, and s4.

[0013] S42. Calculate the normal vectors of planes s1, s2, s3, and s4 respectively. Specifically, it includes the following sub-steps:

[0014] S421. From planes s1, s2, s3, and s4, obtain two vectors for each of their respective planes:

[0015]

[0016] Where f(x,y) represents the size of the pixel in the thermal image data at position (x,y). Let be the unit vectors in the x and y directions, respectively. f(x,y) is a unit vector along the x-axis.

[0017] S422. Based on the two vectors obtained from plane s1, calculate the normal vector of plane s1.

[0018] S423. Calculate the normal vectors of planes s2, s3, and s4 respectively.

[0019]

[0020] S43. The unit vector along the x, y axis of f(x, y) vector Let the included angles be denoted as α1, α2, α3, and α4, and calculate the cosine value of each included angle:

[0021]

[0022] S44. Calculate the sum of the four cosine values ​​γ(i,j), and construct a mask operator based on γ(i,j), specifically including the following sub-steps:

[0023] S441. Calculate the sum of the four cosine values ​​γ(i,j):

[0024]

[0025] S442. Based on the fractional-order differential algorithm, construct the first column of mask coefficients of the horizontal mask operator by combining the sum of cosine values ​​γ(i,j):

[0026]

[0027] Where Γ() is the gamma function and μ is the differential order; the size of the mask operator is (n+2)×(n+2), the fractional coefficients in the mask operator are principal coefficients, and the other coefficients are partial coefficients, the proportion of principal coefficients is β, and the proportion of partial coefficients is (1-β).

[0028] S443. Construct the mask coefficients of the 2nd column, ..., (n+2) / 2th column, ..., n+2th column of the horizontal mask operator in sequence, and form the horizontal mask operator by the mask coefficients of the n+2th column;

[0029] S444. Transpose the horizontal mask operator to obtain the vertical mask operator. Combine the four-leaf vector method to construct a new mask operator based on the horizontal and vertical mask operators.

[0030] S45. The original thermal image of microbial nighttime activity is fed into the constructed mask operator to deblur the thermal image data and obtain an optimized and clear thermal image of microbial nighttime activity.

[0031] S5. The host computer sends the optimized and clear thermal images of microbial nighttime activity to the display device for display and observation.

[0032] Preferably, in step S2, a 12V DC power supply is provided to the thermal imager, and the thermal imager is connected to the host computer for communication.

[0033] On the other hand, the present invention also provides an online observation device for the nighttime activity status of microorganisms based on a thermal imager, which includes an observation device and a host computer. The observation device is equipped with a thermal imager, and the host computer is equipped with a thermal image processing unit. The thermal imager is communicatively connected to the thermal image processing unit.

[0034] The observation device includes a movable support base, a height adjustment module, and a rotation module. The movable support base includes a frame, low center of gravity adjustment wheels, a vertical pole, and an inclined support rod. The low center of gravity adjustment wheels are installed at the four corners of the frame, and the vertical pole is installed in the middle of the frame. The inclined support rod connects the vertical pole to the frame. The height adjustment module is installed on the vertical pole and includes a guide rail and a movable guide rail connecting plate. The movable connecting plate is equipped with a rotation adjustment module and a self-locking device.

[0035] The rotation adjustment module includes a crossbeam, a crossbeam diagonal support rod, a guide rod, and a thermal imager rotation mounting base mounted on a movable connecting plate. The crossbeam is fixed to the movable connecting plate, and the crossbeam diagonal support rod is fixed to both the movable connecting plate and the crossbeam. The guide rod and the thermal imager rotation mounting base are mounted on the crossbeam and connected together. The thermal imager is mounted on the thermal imager rotation mounting base. The movable connecting plate can move up and down on a guide rail and is limited by a self-locking device on the movable connecting plate. Rotating the guide rod can drive the thermal imager rotation mounting base to rotate.

[0036] Preferably, the crossbeam is composed of three crossbeam connecting pipes, which are fixed by a connecting sleeve. The crossbeam connecting pipes are fixed to the movable guide rail connecting plate by screws using corner brackets. The first end of the crossbeam inclined support rod is fixed to the movable guide rail connecting plate by a crossbeam inclined support connecting plate, and the second end of the crossbeam inclined support rod is fixed to the crossbeam by a crossbeam inclined support connecting plate.

[0037] Preferably, the movable support base is a square frame formed by four base frame connecting pipes, and the middle of the frame is a cross frame formed by one base frame connecting pipe and two base frame support connecting pipes.

[0038] Preferably, the thermal image processing unit is provided with a mask operator construction module.

[0039] Preferably, the guide rail is a linear guide rail with a built-in slide rail.

[0040] Preferably, the low center of gravity adjustment wheel includes a low center of gravity adjustment wheel swivel caster and a low center of gravity adjustment wheel support foot.

[0041] Preferably, the movable support base is provided with a counterweight module.

[0042] Preferably, the guide rod is fixed to the crossbeam by three guide rod brackets and one guide rod top bracket. A rotating hinge is installed at the first end of the guide rod, and a guide rod rotating device is installed at the second end of the guide rod. The first end of the rotating hinge is connected to the guide rod, and the second end of the rotating hinge is connected to the thermal imager rotating mounting base.

[0043] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0044] (1) This invention utilizes a thermal imager to achieve online observation of the nocturnal activity of freshwater microorganisms and can acquire high-quality images for observation. In use, the observation area can be adjusted via the movable support base, the height at which the thermal imager acquires data can be changed via the up-and-down adjustment device, and the angle at which the thermal imager acquires the freshwater area can be changed via the rotation module, thereby acquiring as much information as possible about the nocturnal activity areas of microorganisms and taking multiple images for more detailed observation, thus enabling a more detailed analysis of the nocturnal activity patterns of microorganisms.

[0045] (2) This invention utilizes a thermal imager to conduct online observation of the nighttime activity of freshwater microorganisms and transmits the data to a computer via a data cable to display real-time infrared thermal images of the nighttime activity of freshwater microorganisms. It can also process the infrared thermal images to achieve online observation of the nighttime activity trajectory of freshwater microorganisms.

[0046] (3) The observation device of the present invention has a movable support base, an up-down adjustment module and a rotation adjustment module. It can not only observe the change of position online, but also realize the three-dimensional adjustment of the height and angle of the online observation at the position, thereby acquiring more infrared thermal image information, and can adjust the position of the thermal imager in real time as needed.

[0047] (4) When using this invention, the height of the upright, the length of the crossbeam and the weight of the counterweight can be increased as needed, thereby changing the height and size of the entire device. At the same time, the crossbeam and the bracket are easy to disassemble and assemble, making it convenient for storage and transportation.

[0048] (5) The observation method of the present invention adopts a deblurring algorithm based on the four-leaf vector method for processing infrared thermal images, and constructs a new mask operator. The overall processing method can effectively reduce the blurring effect caused by the movement of plankton and improve the quality of online observation. Attached Figure Description

[0049] Figure 1 This is a three-dimensional schematic diagram of the overall assembly of the three-dimensional adjustable online observation device for the nocturnal activity of freshwater microorganisms based on a thermal imager, according to the present invention.

[0050] Figure 2This is a three-dimensional schematic diagram of the movable support base of the present invention;

[0051] Figure 3 This is a three-dimensional schematic diagram of the vertical adjustment module of the present invention;

[0052] Figure 4 This is a left view of the movable guide rail connecting plate of the present invention;

[0053] Figure 5 This is a three-dimensional schematic diagram of the rotation adjustment module of the present invention;

[0054] Figure 6 This is a three-dimensional schematic diagram of the connection between the guide rod and the rotating mounting base of the thermal imager in this invention;

[0055] Figure 7 This is a schematic block diagram illustrating the data information transmission of the observation device of the present invention;

[0056] Figure 8 This is a graph of the raw data acquired by the observation device of the present invention;

[0057] Figure 9 This is a data graph processed by the observation method of the present invention.

[0058] Some of the attached figures are labeled as follows:

[0059] Movable support base 101; height adjustment module 102; rotation adjustment module 103; counterweight module 104;

[0060] 201 Base frame connecting tube; 202 Corner seat; 203 Low center of gravity adjustment wheel; 204 Base frame support connecting tube; 205 Low center of gravity adjustment wheel swivel caster; 206 Low center of gravity adjustment wheel support foot;

[0061] Upright connecting pipe 301; Upright diagonal support connecting pipe 302; Linear guide rail 303; Movable guide rail connecting plate 304; Crossbeam fixing foot 305; Crossbeam diagonal support connecting plate 306; Linear guide rail fixing hole 307; Movable guide rail connecting plate fixing screw hole 308; Guide rod rotating device 309.

[0062] 401. Crossbeam connecting pipe; 402. Guide rod; 403. Crossbeam diagonal support rod; 404. Thermal imager rotating fixing seat; 405. Crossbeam connecting pipe connecting sleeve; 406. Guide rod bracket; 407. Guide rod top bracket; 408. Thermal imager; 409. Rotating hinge. Detailed Implementation

[0063] Hereinafter, embodiments of the present invention will be described with reference to the accompanying drawings.

[0064] It should be noted that, unless otherwise stated, the technical or scientific terms used in this application should have the ordinary meaning understood by one of ordinary skill in the art. For ease of understanding, based on... Figure 1 The orientation of the device is described.

[0065] This invention provides a device for observing the nocturnal activity of microorganisms, and also provides a method for observing the nocturnal activity of microorganisms based on the device.

[0066] Observation device

[0067] Figure 1 This is a three-dimensional adjustable online observation device for the nocturnal activity of freshwater microorganisms based on a thermal imager, as shown in the present invention. Figure 1 As shown, the observation device includes a movable support base 101, a height adjustment module 102, a rotation adjustment module 103, a counterweight module 104 for maintaining the balance of the online observation device, and a host computer connected via a data cable. Each component is described in detail below. Figure 2 As shown, the movable support base 101 includes a base frame connecting tube 201, a low center of gravity adjustment wheel 203, a corner bracket 202, and a base frame support connecting tube 204. The five base frame connecting tubes 201 of equal length are fixed together by the corner bracket 202 with screws. Among them, four base frame connecting tubes 201 are connected to each other to form an outer square frame. Two base frame support connecting tubes 204 are fixed to the remaining base frame connecting tube 201 with screws to form a cross frame, which supports the outer square frame. Each of the four corners of the base frame connecting pipe 201 is equipped with a low center of gravity adjustment wheel 203. The low center of gravity adjustment wheel 203 is fixed to the base frame connecting pipe 201 with screws. The low center of gravity adjustment wheel 203 includes a low center of gravity adjustment wheel universal caster 205 and a low center of gravity adjustment wheel support foot 206. Moving the low center of gravity adjustment wheel universal caster 205 can change the observation position of the three-dimensional adjustable online observation device for the nocturnal activity state of freshwater microorganisms. The low center of gravity adjustment wheel support foot 206 can fix the online observation position. When it is necessary to move the observation device, the low center of gravity adjustment wheel universal caster 205 can be used to move the entire observation device. After moving it to the designated position, the low center of gravity adjustment wheel support foot 206 can be used to fix the observation device.

[0068] like Figure 3 and Figure 4As shown, the vertical adjustment module 102 of the three-dimensional adjustable online observation device for the nocturnal activity of freshwater microorganisms includes a pole connecting pipe 301, an inclined support connecting pipe 302, a linear guide rail 303, and a movable guide rail connecting plate 304, all mounted on a movable support base 101. The pole connecting pipe 301 is fixed to the movable support base 101 with screws via an angle bracket 202. The inclined support connecting pipe 302 is fixed to the movable support base 101 and the pole connecting pipe 301 with screws to ensure the stability of the pole connecting pipe 301. The linear guide rail 303 is fixed to the upright connecting pipe 301 by screws through eight linear guide rail fixing holes 307. The movable guide rail connecting plate 304 is fixed to the linear guide rail 303 by screws through movable guide rail connecting plate fixing screw holes 308. The vertical position of the thermal imager can be adjusted by moving the movable guide rail connecting plate 304 up and down. The movable guide rail connecting plate 304 has a self-locking device, which can fix the vertical position of the thermal imager 408. The movable guide rail connecting plate 304 is equipped with a crossbeam fixing foot 305, a crossbeam inclined support connecting plate 306, and a guide rod rotating device 309. When it is necessary to adjust the vertical position of the thermal imager, the movable guide rail connecting plate 304 can be moved up and down. After moving to the target height position, the self-locking device of the movable guide rail connecting plate 304 is used to fix its position, so that the thermal imager is fixed in the required position.

[0069] like Figure 5 and Figure 6As shown, the rotation adjustment module 103 of the three-dimensional adjustable online observation device for the nocturnal activity of freshwater microorganisms includes three crossbeam connecting pipes 401, guide rods 402, crossbeam inclined support rods 403, and a thermal imager rotating fixing seat 404. The three crossbeam connecting pipes 401 are connected together by crossbeam connecting pipe connecting sleeves 405. The crossbeam connecting pipes 401 are fixed to the movable guide rail connecting plate 304 by screws from the corner brackets 202. One end of the crossbeam inclined support rod 403 is fixed to the movable guide rail connecting plate 304 by the crossbeam inclined support connecting plate 306, and the other end of the crossbeam inclined support rod 403 is fixed to the crossbeam connecting pipe 401 to ensure the stability of the crossbeam connecting pipe 401. The guide rods 402 are supported by three guide rod brackets 406. A guide rod top bracket 407 is fixed to the crossbeam connecting pipe 401. One end of the guide rod 402 is equipped with a rotating hinge 409, and the other end is equipped with a guide rod rotating device 309. One end of the rotating hinge 409 is connected to the guide rod 402, and the other end is connected to the thermal imager rotating mounting base 404. The thermal imager 408 is mounted on the thermal imager rotating mounting base 404. When the guide rod rotating device 309 rotates, it can drive the thermal imager rotating mounting base 404 to rotate. By rotating the guide rod rotating device 309, the angle of the thermal imager rotating mounting base 404 can be quickly changed, thereby adjusting the observation angle of the thermal imager 408. During use, the angle of the thermal imager 408 can be quickly adjusted as needed to obtain images from more different angles. In this embodiment, all the connecting pipes and the crossbeam diagonal support rods mentioned above are made of aluminum profiles.

[0070] The online observation device of the present invention can not only move the online observation position through the movable support base 101, but also realize the three-dimensional adjustment of the thermal imager 408 through the up-down adjustment module 102 and the rotation adjustment module 103. In this way, it can realize online observation of the nocturnal activity of microorganisms in freshwater areas at different locations and angles, and acquire multiple observation thermal images to understand the community composition and changes of plankton, and provide data support for revealing the eutrophication level of lakes and water quality changes.

[0071] like Figure 7 As shown, the thermal image data acquired by the thermal imager 408 is transmitted to the computer for data processing via a network cable. The thermal imager 408 requires a 12V DC power supply. The processed thermal image data can be directly displayed on the screen for easy observation.

[0072] like Figure 8 As shown, the original image is shown before data processing. The thermal image data acquired by the thermal imager 408 is blurred due to the movement of plankton.

[0073] like Figure 9As shown, the thermal image data acquired by the thermal imager 408 becomes clear after being processed by the observation method proposed in this patent, laying the foundation for improving the observation quality of the nocturnal activity of plankton.

[0074] The specific usage process of this device is as follows:

[0075] When using this device, firstly, the observation device is moved to the desired online observation position via the low center of gravity adjustment wheels 203 on the movable support base 101. After reaching the designated position, the online observation position is fixed by the low center of gravity adjustment wheel support feet 206 at the bottom. During the movement, it is important to ensure that the counterweight module 104 moves together to ensure that the online observation device does not tilt. Then, the vertical position of the thermal imager 408 is adjusted by moving the movable guide rail connecting plate 304 on the vertical adjustment module 102, and its vertical position is fixed by the self-locking device of the movable guide rail connecting plate 304 to lock the vertical coordinate. Afterward, the angle of the thermal imager is adjusted by rotating the adjustment module 103 to adjust the angle for acquiring thermal image data. Specifically, the guide rod rotation device 309 rotates the guide rod 402 to drive the thermal imager rotating fixed base 404 to rotate, thereby driving the thermal imager 408 to rotate and adjusting the angle for acquiring thermal image data. After the observation position of the thermal imager 408 is determined, a 12V DC power supply is provided to the thermal imager 408, and the thermal imager 408 is connected to the computer via a network cable. The computer processes the data and displays the nocturnal activity status of freshwater microorganisms on the screen in real time. During the observation process, the vertical position and angle of the thermal imager can be adjusted at any time, thereby acquiring images from various angles for detailed observation and a full understanding of the nocturnal activity status of microorganisms. In summary, the online observation device of this invention can achieve three-dimensional adjustment of the observation device through the movable support base 101, the vertical adjustment module 102, and the rotation adjustment module 103 to comprehensively acquire raw data on the nocturnal activity of freshwater microorganisms. After processing by the method proposed in this patent, clear thermal image data is obtained, thereby realizing online observation of the nocturnal activity of freshwater microorganisms.

[0076] Observation methods

[0077] On the other hand, the present invention provides an online observation method for the nighttime activity status of microorganisms based on a thermal imager, which includes the following steps:

[0078] S1. Move the observation device to the location for online observation and adjust the height and angle of the thermal imager, specifically including the following sub-steps:

[0079] S11. Move the observation device to a horizontal position for online observation using the low center of gravity adjustment wheels of the movable support base.

[0080] S12. Adjust the height of the thermal imager by moving the movable guide rail connecting plate on the up-down adjustment module, and fix the height position by using the self-locking device of the movable guide rail connecting plate.

[0081] S13. The guide rod rotation device of the rotating module drives the thermal imager rotating base to rotate, thereby driving the thermal imager to rotate and adjusting the angle of the thermal imager.

[0082] S2. Power on the thermal imager and connect it to the host computer. The thermal imager acquires thermal images of microbial activity at night and uploads them to the host computer.

[0083] S3. During the observation process, the height of the thermal imager is changed by adjusting the height and / or the angle of the thermal imager is changed by rotating the module to obtain multiple original thermal images of microbial nighttime activity.

[0084] S4. The original thermal image of nighttime microbial activity is processed using the thermal image processing unit inside the host computer to obtain a clear image, which includes the following sub-steps:

[0085] S41. Based on the original thermal images of microbial nighttime activity, a new mask operator is constructed using the four-leaf vector method. i,j This represents the thermal image data point at position (i,j); it will be compared with A i,j Eight adjacent thermal image data points are divided into four regions, and the two thermal image data points in each region are related to A. i,j They form a plane, namely s1, s2, s3, and s4.

[0086] S42. Calculate the normal vectors of planes s1, s2, s3, and s4 respectively. Specifically, it includes the following sub-steps:

[0087] S421. From planes s1, s2, s3, and s4, obtain two vectors for each of their respective planes:

[0088]

[0089] Where f(x,y) represents the size of the pixel in the thermal image data at position (x,y). Let be the unit vectors in the x and y directions, respectively. f(x,y) is a unit vector along the x-axis.

[0090] S422. Based on the two vectors obtained from plane s1, calculate the normal vector of plane s1.

[0091]

[0092] S423. Calculate the normal vectors of planes s2, s3, and s4 respectively.

[0093]

[0094] S43. The unit vector along the x, y axis of f(x, y) vector Let the included angles be denoted as α1, α2, α3, and α4, and calculate the cosine value of each included angle:

[0095]

[0096] S44. Calculate the sum of the four cosine values ​​γ(i,j), and construct a mask operator based on γ(i,j), specifically including the following sub-steps:

[0097] S441. Calculate the sum of the four cosine values ​​γ(i,j):

[0098]

[0099] S442. Based on the fractional-order differential algorithm, construct the first column of mask coefficients of the horizontal mask operator by combining the sum of cosine values ​​γ(i,j):

[0100]

[0101] Where Γ() is the gamma function and μ is the differential order; the size of the mask operator is (n+2)×(n+2), the fractional coefficients in the mask operator are principal coefficients, and the other coefficients are partial coefficients, the proportion of principal coefficients is β, and the proportion of partial coefficients is (1-β).

[0102] S443. Construct the mask coefficients of the 2nd column, ..., (n+2) / 2th column, ..., n+2th column of the horizontal mask operator in sequence, and the horizontal mask operator is formed by the mask coefficients of the n+2th column.

[0103] S444. Transpose the horizontal mask operator to obtain the vertical mask operator. Combine the four-leaf vector method to construct a new mask operator based on the horizontal and vertical mask operators.

[0104] S45. The original thermal image of microbial nighttime activity is fed into the constructed mask operator to deblur the thermal image data and obtain an optimized and clear thermal image of microbial nighttime activity.

[0105] S5. The host computer sends the optimized and clear thermal images of microbial nighttime activity to the display device for display and observation.

[0106] The apparatus and method of the present invention will be described in detail below with reference to specific embodiments:

[0107] In this embodiment, in order to better observe the nocturnal activities of freshwater microorganisms (plankton), the observation device of the present invention is used to acquire thermal images of microbial activity and process the thermal images to obtain clear thermal images for detailed observation.

[0108] A method for observing the nocturnal activity of freshwater microorganisms, comprising the following steps:

[0109] S1. Use the casters to move the observation device to the location for online observation and adjust the position of the thermal imager to the optimal observation position.

[0110] First, the observation device is moved to the position for online observation using the low center of gravity adjustment wheels on the movable support base.

[0111] Secondly, after the entire device is moved to the observation position, the height of the thermal imager is adjusted by moving the movable guide rail connecting plate on the up-down adjustment module, and the up-down position is fixed by the self-locking device of the movable guide rail connecting plate, thus completing the height adjustment of the thermal imager and placing the thermal imager at the closest observation height.

[0112] Finally, the guide rod of the guide rod rotation device drives the thermal imager's rotating base to rotate, thereby rotating the thermal imager and adjusting its angle to achieve the optimal observation angle.

[0113] S2. Power on the thermal imager and connect it to the host computer. Use the thermal imager to acquire thermal images of microbial activity at night and upload them to the host computer.

[0114] S3. When needed, the position of the observation device can be moved using the movable support base. The height of the thermal imager can be changed by adjusting the height and / or the angle of the thermal imager can be changed by rotating the module to obtain multiple original thermal images of microbial nighttime activity for more detailed observation and analysis of the nighttime activity patterns of microorganisms.

[0115] S4. A new mask operator, constructed using the thermal image processing unit inside the host computer, is used to process the original thermal image of nighttime microbial activity to obtain a clear image. The original image is shown below. Figure 8 As shown, the processed image is clear. Figure 9 As shown, the processed, clear images clearly show the nocturnal activity of microorganisms, and analyzing the thermal images can help to understand the community composition and changes of plankton.

[0116] The embodiments described above are merely preferred embodiments of the present invention and are not intended to limit the scope of the present invention. Various modifications and improvements made by those skilled in the art to the technical solutions of the present invention without departing from the spirit of the present invention should fall within the protection scope defined by the claims of the present invention.

Claims

1. A method for online observation of microbial nighttime activity based on thermal imaging, characterized in that: It includes the following steps: S1. Move the observation device to the location for online observation and adjust the height and angle of the thermal imager, specifically including the following sub-steps: S11. Move the observation device to a horizontal position for online observation using the low center of gravity adjustment wheels of the movable support base; S12. Adjust the height of the thermal imager by moving the movable guide rail connecting plate on the up-down adjustment module, and fix the height position by the self-locking device of the movable guide rail connecting plate. S13. The guide rod rotating device of the rotating module drives the thermal imager rotating fixed base to rotate, thereby driving the thermal imager to rotate and adjusting the angle of the thermal imager; S2. Power on the thermal imager and connect it to the host computer. The thermal imager acquires thermal images of microbial activity at night and uploads them to the host computer. S3. During the observation process, the height of the thermal imager is changed by the up-down adjustment device and / or the angle of the thermal imager is changed by the rotation module to obtain multiple original thermal images of microbial nighttime activity. S4. The original thermal image of nighttime microbial activity is processed using the thermal image processing unit inside the host computer to obtain a clear image, which includes the following sub-steps: S41. Based on the original thermal images of microbial nighttime activity, a new mask operator is constructed using the four-leaf vector method. i,j This represents the thermal image data point at position (i,j); it will be compared with A i,j Eight adjacent thermal image data points are divided into four regions, and the two thermal image data points in each region are related to A. i,j They form a plane, namely s1, s2, s3, and s4; S42. Calculate the normal vectors of planes s1, s2, s3, and s4 respectively. Specifically, it includes the following sub-steps: S421. From planes s1, s2, s3, and s4, obtain two vectors for each of their respective planes: Where f(x,y) represents the size of the pixel in the thermal image data at position (x,y). Let be the unit vectors in the x and y directions, respectively. f(x,y) is a unit vector along the x-axis. S422. Based on the two vectors obtained from plane s1, calculate the normal vector of plane s1. S423. Calculate the normal vectors of planes s2, s3, and s4 respectively. S43. The unit vector along the x, y axis of f(x, y) vector Let the included angles be denoted as α1, α2, α3, and α4, and calculate the cosine value of each included angle: S44. Calculate the sum of the four cosine values ​​γ(i,j), and construct a mask operator based on γ(i,j), specifically including the following sub-steps: S441. Calculate the sum of the four cosine values ​​γ(i,j): S442. Based on the fractional-order differential algorithm, construct the first column of mask coefficients of the horizontal mask operator by combining the sum of cosine values ​​γ(i,j): Where Γ() is the gamma function and μ is the differential order; the size of the mask operator is (n+2)×(n+2), the fractional coefficients in the mask operator are principal coefficients, and the other coefficients are partial coefficients, the proportion of principal coefficients is β, and the proportion of partial coefficients is (1-β). S443. Construct the mask coefficients of the 2nd column, ..., (n+2) / 2th column, ..., n+2th column of the horizontal mask operator in sequence, and form the horizontal mask operator by the mask coefficients of the n+2th column; S444. Transpose the horizontal mask operator to obtain the vertical mask operator. Combine the four-leaf vector method to construct a new mask operator based on the horizontal and vertical mask operators. S45. The original thermal image of microbial nighttime activity is fed into the constructed mask operator to deblur the thermal image data and obtain an optimized and clear thermal image of microbial nighttime activity. S5. The host computer sends the optimized and clear thermal images of microbial nighttime activity to the display device for display and observation.

2. The online observation method for the nighttime activity status of microorganisms based on thermal imaging as described in claim 1, characterized in that: In step S2, a 12V DC power supply is provided to the thermal imager, and the thermal imager is connected to the host computer for communication.

3. An online observation device for the online observation method of microbial nighttime activity status based on thermal imager as described in claim 1, characterized in that: It includes an observation device and a host computer. The observation device is equipped with a thermal imager, and the host computer is equipped with a thermal image processing unit. The thermal imager is communicatively connected to the thermal image processing unit. The observation device includes a movable support base, a height adjustment module, and a rotation module. The movable support base includes a frame, low center of gravity adjustment wheels, a vertical pole, and an inclined support rod. The low center of gravity adjustment wheels are installed at the four corners of the frame, and the vertical pole is installed in the middle of the frame. The inclined support rod connects the vertical pole to the frame. The height adjustment module is installed on the vertical pole and includes a guide rail and a movable guide rail connecting plate. The movable connecting plate is equipped with a rotation adjustment module and a self-locking device. The rotation adjustment module includes a crossbeam, a crossbeam diagonal support rod, a guide rod, and a thermal imager rotation mounting base mounted on a movable connecting plate. The crossbeam is fixed to the movable connecting plate, and the crossbeam diagonal support rod is fixed to both the movable connecting plate and the crossbeam. The guide rod and the thermal imager rotation mounting base are mounted on the crossbeam and connected together. The thermal imager is mounted on the thermal imager rotation mounting base. The movable connecting plate can move up and down on a guide rail and is limited by a self-locking device on the movable connecting plate. Rotating the guide rod can drive the thermal imager rotation mounting base to rotate.

4. The online observation device for nighttime microbial activity based on a thermal imager according to claim 3, characterized in that: The crossbeam consists of three crossbeam connecting pipes, which are fixed by connecting sleeves. The crossbeam connecting pipes are fixed to the movable guide rail connecting plate by screws using corner brackets. The first end of the crossbeam inclined support rod is fixed to the movable guide rail connecting plate by the crossbeam inclined support connecting plate, and the second end of the crossbeam inclined support rod is fixed to the movable guide rail connecting plate by the crossbeam inclined support connecting plate.

5. The online observation device for nighttime microbial activity based on a thermal imager according to claim 3, characterized in that: The movable support base is a square frame formed by four base frame connecting pipes, and the middle of the frame is a cross frame formed by one base frame connecting pipe and two base frame support connecting pipes.

6. The online observation device for nighttime microbial activity based on a thermal imager according to claim 3, characterized in that: The thermal image processing unit is equipped with a mask operator construction module.

7. The online observation device for nighttime microbial activity based on a thermal imager according to claim 3, characterized in that: The guide rail is a linear guide rail with built-in slide rail.

8. The online observation device for nighttime microbial activity based on a thermal imager according to claim 3, characterized in that: The low center of gravity adjustment wheel includes a low center of gravity adjustment wheel swivel caster and a low center of gravity adjustment wheel support foot.

9. The online observation device for nighttime microbial activity based on a thermal imager according to claim 3, characterized in that: The movable support base is equipped with a counterweight module.

10. The online observation device for nighttime microbial activity based on a thermal imager according to claim 3, characterized in that: The guide rod is fixed to the crossbeam by three guide rod brackets and one guide rod top bracket. A rotating hinge is installed at the first end of the guide rod, and a guide rod rotating device is installed at the second end of the guide rod. The first end of the rotating hinge is connected to the guide rod, and the second end of the rotating hinge is connected to the thermal imager rotating mounting base.

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