A viral detection assay device

Abstract

The application provides a virus detection and analysis device, which comprises a sample adding module and a detection module; the sample adding module is used for sucking a liquid sample in a test tube and injecting the liquid sample onto a detection card; the sample adding module comprises a sampling unit, the sampling unit comprises a sampling pump, a sampling tube, a control valve and a controller, and a first sensor and a second sensor are further fixed outside the sampling tube; the first sensor and the second sensor are respectively used for detecting the medium form in the sampling tube at the corresponding positions; the controller controls the sampling pump and the control valve to work according to the detection signals output by the first sensor and the second sensor; the detection module is used for acquiring a reaction image of the detection card, performing image processing and analysis, and outputting an analysis result. The application effectively judges the bubble section and the air section generated by sampling and performs filtering, ensures that each sampling can meet the detection requirements, and improves the detection accuracy of the virus detection and analysis instrument.

Description

Technical Field

[0001] This invention belongs to the field of medical testing device technology, specifically relating to a virus detection and analysis device. Background Technology

[0002] Virus testing and analysis has become a routine testing method in the medical testing industry. Specifically, it involves collecting tissues or bodily fluids from the human body, generating a test liquid in a test tube, and then injecting the test liquid into a test card. The test sample and the test card react differently, and the test results can determine whether the human body is infected with a virus.

[0003] In existing virus detection and analysis technologies, testing personnel typically manually inject the test liquid from a test tube into a test card. Because some viruses are infectious, such as syphilis, SARS, and Ebola, testing personnel face a risk of infection during the testing process. Therefore, existing technologies have also developed a fully automated virus detection and analysis instrument that realizes the entire process from sample identification to result acquisition, avoiding human contact during virus detection and ensuring the safety of testing personnel.

[0004] In practical use, due to the inconsistent liquid level of the liquid being tested, fully automated virus detection and analysis instruments are prone to introducing air bubbles and air gaps when sampling from the liquid. This results in the sampling volume not reaching the standard sampling volume, leading to low accuracy of the test results. Therefore, there is an urgent need for a virus detection and analysis instrument that can effectively identify and filter the air bubbles and air gaps generated during sampling, ensuring that the standard sampling volume is reached every time, and improving the detection accuracy of the virus detection and analysis instrument. Summary of the Invention

[0005] The present invention aims to solve the technical problems existing in the prior art and provide a virus detection and analysis device that can effectively identify and filter the bubble segment and air segment generated during sampling, ensuring that each sample meets the detection requirements and improving the detection accuracy of the virus detection and analysis instrument.

[0006] To achieve the above-mentioned objectives of the present invention, according to a first aspect of the present invention, a virus detection and analysis device is provided, comprising a sample application module and a detection module; the sample application module is used to draw liquid samples from a test tube and inject the liquid samples onto a detection card; the sample application module includes a sampling unit, which includes a sampling pump, a sampling tube, a control valve, and a controller, and a first sensor and a second sensor are fixed outside the sampling tube; the distance between the first sensor and the second sensor on the sampling tube is calculated based on the standard sampling volume and the diameter of the sampling tube; the first sensor and the second sensor are respectively used to detect the morphology of the medium in the sampling tube at their corresponding positions, the medium morphology including liquid, bubbles, and air; the controller controls the operation of the sampling pump and the control valve according to the detection signals output by the first sensor and the second sensor; the detection module is used to acquire the reaction image of the detection card and perform image processing analysis, and output the analysis results.

[0007] Furthermore, both the first and second sensors include photoelectric sensors, which output corresponding detection signals based on the morphology of the medium inside the sampling tube. The sampling process for the liquid sample is as follows: the controller controls the sampling pump to start sampling, and the first and second sensors respectively read the morphology of the medium at the corresponding position on the sampling tube and output detection signals; the controller reads the detection signals output by the first and second sensors in real time, obtains the morphology of the medium at the corresponding position based on the detection signals, and counts the number of changes in the detection signals of the first and second sensors in real time; when the morphology of the medium at the corresponding position of the first and second sensors is liquid, and the number of changes in the detection signals of the first and second sensors is equal, the controller controls the sampling pump to stop and closes the control valve; the process of injecting the liquid sample into the detection card is as follows: the controller opens the control valve and controls the sampling pump to start draining the liquid; when the detection signal of the first sensor changes once, the controller controls the sampling pump to stop draining the liquid and closes the control valve.

[0008] Furthermore, both the first and second sensors also include filtering circuits, which are used to filter out interference signals in the detection signal. A comparator is also connected between the first and second sensors and the controller. The comparator is used to output a digital signal based on the detection signal obtained by the photoelectric sensor. The controller combines the digital signal and the filtered detection signal to control the operation of the sampling pump and the control valve.

[0009] Furthermore, the sample addition module also includes a cleaning unit, which is used to clean the sample addition unit. The cleaning unit includes a cleaning device, one end of which has a cleaning inlet, and the other end of which is connected to a waste liquid pipeline, on which a waste discharge pump is installed. A cleaning pipeline is connected next to the cleaning inlet of the cleaning device, and the other end of the cleaning pipeline is connected to a cleaning solution storage chamber, on which a cleaning pump is also installed. A negative pressure device is also installed at the cleaning inlet of the cleaning device. The negative pressure device, the cleaning pump, and the waste discharge pump are electrically connected to the controller.

[0010] Further, the cleaning process is as follows: The sampling tube is inserted into the cleaning device through the cleaning inlet. The controller starts the cleaning pump to draw cleaning fluid from the cleaning fluid storage chamber into the cleaning device to clean the outer wall of the sampling tube. After the outer wall cleaning is completed, the waste pump is started to extract the cleaning fluid from the cleaning device. The cleaning pump is then started again to draw cleaning fluid into the cleaning device. The sampling pump is started to draw cleaning fluid from the cleaning device into the sampling tube to clean the inner wall of the sampling tube. When the absorption time of the cleaning fluid is longer than the sampling time, the controller controls the sampling pump to discharge the cleaning fluid into the cleaning device. The waste pump is started to draw the cleaning fluid from the waste liquid pipe. The negative pressure device is started to extract the residual cleaning fluid on the sampling tube.

[0011] Furthermore, the sample dispensing module also includes a shaking unit; the shaking unit is used to shake the test card containing the injected liquid sample.

[0012] Furthermore, the detection module includes a processing unit and an analysis unit; the processing unit is used to acquire the reaction image of the detection card and perform binarization processing on the reaction image, and the analysis unit obtains the detection result based on the processed reaction image.

[0013] Furthermore, it also includes a pre-processing module for pre-processing samples and fixing test tubes containing liquid samples; an identification unit for identifying whether there are test tubes in the area to be inspected; a gripping unit for gripping and rotating test tubes; and a scanning unit for scanning the identification code on the test tubes.

[0014] Furthermore, the pretreatment module also includes a cap removal unit, which is used to remove the cap from the gripped test tube and to seal the test tube after the sampling process is completed.

[0015] Furthermore, it also includes a recycling module; the recycling module is used to seal and recycle the test tubes and test cards after testing.

[0016] The technical principle and beneficial effects of this invention: In this solution, the sampling tube of the sampling module draws liquid samples from the test tube. Since different forms of media absorb light signals differently, the first sensor outside the sampling tube obtains the corresponding detection results, i.e., voltage values, according to different forms. The controller determines whether the medium in the sampling tube is liquid, air, or bubbles based on the changes and ranges of the voltage values ​​of the first and second sensors. The first sensor records the liquid, bubble, and air segments that have passed through. The second sensor filters the bubble, air, and small liquid segments until they have completely passed through the second sensor. Since the distance between the first and second sensors is calculated based on the standard sampling volume and the diameter of the sampling tube, the liquid between the first and second sensors has reached the standard sampling volume, meeting the detection requirements and improving the detection accuracy of the virus analyzer. Attached Figure Description

[0017] Figure 1 This is a schematic diagram of the structure of a virus detection and analysis device according to the present invention;

[0018] Figure 2 This is a schematic diagram of the sampling unit of the present invention;

[0019] Figure 3 This is a schematic diagram of the sampling unit and cleaning unit of the present invention;

[0020] Figure 4 This is a schematic diagram of the syphilis detection card test of the present invention;

[0021] Figure 5 This is a schematic diagram of the syphilis detection card test of the present invention;

[0022] Figure 6 This is a schematic diagram of the syphilis detection card test of the present invention.

[0023] Reference numerals in the attached drawings: 1. First sensor; 2. Second sensor; 3. Sampling tube; 4. Sampling pump; 5. Control valve; 6. First section of medium bubble section; 7. Second section of medium liquid section; 8. Negative pressure device; 9. Cleaning device; 10. Cleaning pump; 11. Cleaning liquid storage chamber; 12. Cleaning sensor; 13. Waste liquid pipeline; 14. Waste discharge pump; 15. Cleaning pipeline. Detailed Implementation

[0024] In the description of this invention, it should be understood that the terms "longitudinal", "lateral", "up", "down", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this invention.

[0025] In the description of this invention, unless otherwise specified and limited, it should be noted that the terms "installation", "connection" and "linking" should be interpreted broadly. For example, they can refer to mechanical or electrical connections, or internal connections between two components. They can be direct connections or indirect connections through an intermediate medium. Those skilled in the art can understand the specific meaning of the above terms according to the specific circumstances.

[0026] As attached Figure 1 As shown, the present invention provides a virus detection and analysis device, including a sample addition module and a detection module;

[0027] The sample dispensing module is used to draw liquid samples from a test tube and inject the liquid samples onto the test card; the sample dispensing module includes a sampling unit; as shown in the attached document. Figure 2 As shown, the sampling unit includes a sampling pump 4, a sampling tube 3, a control valve 5, and a controller. A first sensor 1 and a second sensor 2 are also fixed outside the sampling tube 3. The distance between the first sensor 1 and the second sensor 2 on the sampling tube 3 is calculated based on the standard sampling volume and the diameter of the sampling tube 3. The calculation process is as follows: Let the standard sampling volume be V, and the inner radius of the test tube be r, then the distance between the first sensor 1 and the second sensor 2...

[0028] Specifically, the control valve 5, the first sensor 1, the second sensor 2, and the sampling pump 4 are electrically connected to the controller; the first sensor 1 and the second sensor 2 are used to detect the state of the medium in the sampling tube 3 at their respective positions, and the state of the medium includes liquid, bubbles and air; the controller controls the operation of the sampling pump 4 and the control valve 5 according to the detection signals output by the first sensor 1 and the second sensor 2.

[0029] In this embodiment, both the first sensor 1 and the second sensor 2 include photoelectric sensors. The photoelectric sensors are preferably high-sensitivity reflective photoelectric sensors or direct photoelectric sensors. The reflective photoelectric sensors and their matching reflectors are axially symmetrically mounted on both sides of the test tube. The transmitter and receiver of the direct photoelectric sensors are axially symmetrically mounted on both sides of the test tube.

[0030] Specifically, because different forms of media absorb light signals differently, the absorption is minimal when the medium is air, increases when the medium is a bubble, and is most abundant (compared to air and bubbles) when the medium is liquid. A highly sensitive photoelectric sensor outputs different detection signals, i.e., different voltage values, based on the light signal absorbed by the medium. For example, two standard thresholds can be set: a first threshold greater than a second threshold. When the voltage output by the photoelectric sensor is greater than the first threshold, the medium at that location is determined to be air; when the voltage output is less than the first threshold but greater than the second threshold, the medium at that location is determined to be a bubble; and when the output voltage is less than the second threshold, the medium at that location is determined to be liquid. These two standard thresholds can be obtained through multiple experiments.

[0031] In this embodiment, when impurities or tiny bubbles are present in the liquid sample, interference signals may exist in the detection signal. In order to improve the accuracy of the signals collected by the first sensor 1 and the second sensor 2, both the first sensor 1 and the second sensor 2 also include a filtering circuit. The filtering circuit is used to filter out interference signals in the detection signal. A comparator is also connected between the first sensor 1, the second sensor 2 and the controller. The comparator is used to output a digital signal based on the detection signal obtained by the photoelectric sensor. The controller is specifically used to control the opening and closing of the sampling pump 4 and the control valve 5 by combining the digital signal and the filtered detection signal.

[0032] The liquid sample collection process is as follows: The controller starts the sampling pump 4 to begin sampling. The first sensor 1 and the second sensor 2 read the medium morphology at the corresponding position on the sampling tube 3 and output detection signals. The controller reads the detection signals output by the first sensor 1 and the second sensor 2 in real time and obtains the medium morphology at the corresponding position based on the detection signals. That is, the medium morphology in the sampling tube 3 at the corresponding position of the first sensor 1 is obtained based on the detection signal of the first sensor 1, and the medium morphology in the sampling tube 3 at the corresponding position of the second sensor 2 is obtained based on the detection signal of the second sensor 2. The controller also counts the number of changes in the detection signals of the first sensor 1 and the second sensor 2 in real time. When the medium morphology at the corresponding positions of the first sensor 1 and the second sensor 2 is both liquid, and the number of changes in the detection signals of the first sensor 1 and the second sensor 2 is equal, the controller stops the sampling pump 4 and closes the control valve 5.

[0033] The process of injecting liquid samples into the test card is as follows: The controller opens the control valve 5 and controls the sampling pump 4 to start draining the liquid. When the detection signal of the first sensor 1 changes once (that is, after the sampling is completed, the detection signal of the first sensor 1 changes once again), the controller controls the sampling pump 4 to stop draining the liquid and the control valve 5 closes.

[0034] In this embodiment, the controller is also used to record the time interval between two consecutive changes in the detection signals of the first sensor 1 or the second sensor 2, and can also calculate the length of the medium passing through the sampling tube 3 in a certain time interval based on parameters such as the flow rate and rotation speed of the sampling pump 4 and the diameter of the sampling tube 3.

[0035] In this embodiment, the sampling process is illustrated in detail with examples of three sampling scenarios:

[0036] Scenario 1: No bubbles or air are generated during sampling, and a liquid sample is obtained directly;

[0037] Scenario 2: When bubbles are generated during sampling, the sample in bubble form is obtained first, and then the sample in liquid form is obtained.

[0038] Scenario 3: During sampling, bubbles are generated and air is mixed in. First, a sample in the form of bubbles is obtained, then a small amount of liquid sample is obtained, then air is mixed in, and finally a liquid sample is obtained.

[0039] The sampling process for scenario ① is as follows:

[0040] The controller starts the sampling pump 4 to begin sampling. The first sensor 1 and the second sensor 2 acquire the state of the medium in the sampling tube 3 in real time and output detection signals. When the liquid sample is drawn into the first sensor 1, the detection signal of the first sensor 1 changes for the first time, at which point the medium state of the first sensor 1 is liquid; the controller marks the first segment of medium as liquid and starts timing.

[0041] When the first segment of medium reaches the second sensor 2, the detection signal of the second sensor 2 changes for the first time, and the detection signal of the first sensor 1 does not change again during this period. The timing stops. At this time, the medium state corresponding to the detection signals of the first sensor 1 and the second sensor 2 is liquid. The detection signals of the first sensor 1 and the second sensor 2 change once each. It can be concluded that the first segment of medium from the first sensor 1 to the second sensor 2 is all liquid and there are no bubbles or air (since the distance from the first sensor 1 to the second sensor 2 is calculated based on the standard sampling volume, the fact that the liquid between the first sensor 1 and the second sensor 2 is all liquid indicates that the liquid segment has reached the standard sampling volume). The controller controls the sampling pump 4 to stop sampling and closes the control valve 5.

[0042] The sampling process for scenario ② is as follows:

[0043] As attached Figure 2As shown, the controller controls the sampling pump 4 to start sampling. The first sensor 1 and the second sensor 2 acquire the state of the medium in the sampling tube 3 in real time and output detection signals. When the liquid sample is drawn into the first sensor 1, the detection signal of the first sensor 1 changes for the first time. At this time, the medium state of the first sensor 1 is bubble-like. The controller marks the first segment of the medium as the bubble segment and starts timing. Before the first segment of the medium reaches the second sensor 2, the detection signal of the first sensor 1 changes for the second time. After the second change, the corresponding medium state is liquid. The controller marks the second segment of the medium as the liquid segment. The controller records the interval between the first change and the second change of the first sensor 1, that is, the elapsed time of the first segment of the medium bubble segment 6 with the first sensor 1 as the reference. The controller can also calculate the elapsed length of the first segment of the medium bubble segment 6 based on the interval time.

[0044] When the first segment of medium reaches the second sensor, the detection signal of the second sensor 2 changes for the first time, and during this period, the detection signal of the first sensor 1 changes for the second time. The medium after the second change is the second segment of medium liquid segment 7. Although the medium form corresponding to the detection signal of the first sensor 1 is liquid, the medium form corresponding to the detection signal of the second sensor 2 is bubbles. Moreover, the detection signals of the first sensor 1 and the second sensor 2 change at different times. Therefore, the sampling pump 4 continues to sample.

[0045] Based on the time or length of the first medium bubble segment 6, after the first medium bubble segment 6 has completely passed the second sensor, the detection signal of the second sensor 2 changes for the second time. At this time, the medium state corresponding to the detection signals of the first sensor 1 and the second sensor 2 is liquid. During this period, the detection signal of the first sensor 1 does not change for the third time. The detection signals of the first sensor 1 and the second sensor 2 change twice. Therefore, it can be concluded that the first medium segment from the first sensor 1 to the second sensor 2 is entirely liquid, without bubbles or air. The controller controls the sampling pump 4 to stop sampling and closes the control valve 5.

[0046] The sampling process for scenario ③ is as follows:

[0047] The controller starts the sampling pump 4 to begin sampling. The first sensor 1 and the second sensor 2 acquire the state of the medium in the sampling tube 3 in real time and output detection signals. When the liquid sample is drawn into the first sensor 1, the detection signal of the first sensor 1 changes for the first time. At this time, the medium state of the first sensor 1 is bubble-like. The controller marks the first segment of the medium as the bubble segment and starts timing. Before the first segment of the medium reaches the second sensor, the detection signal of the first sensor 1 changes for the third time. After the second change, the corresponding medium state is liquid, and the controller marks the first segment of the medium as the bubble segment. After the third change, the corresponding medium state is air, and the controller marks the second segment of the medium as the liquid segment. The controller records the interval between the first and second changes of the first sensor 1, and the interval between the second and third changes, that is, the elapsed time of the first segment of the medium bubble segment and the elapsed time of the second segment of the medium liquid segment with the first sensor as the reference. The controller can also calculate the elapsed length of the first segment of the medium bubble segment and the second segment of the medium liquid segment based on the interval time.

[0048] When the first segment of medium bubbles reaches the second sensor, the detection signal of the second sensor 2 changes for the first time. The medium form corresponding to the detection signal of the first sensor 1 is air, but the medium form corresponding to the detection signal of the second sensor 2 is bubbles. Moreover, the number of changes in the detection signals of the first sensor 1 and the second sensor 2 is different. Therefore, the sampling pump 4 continues to sample.

[0049] When the second liquid medium reaches the second sensor 2, the detection signal of the second sensor 2 changes for the second time. During this period, the detection signal of the first sensor 1 has changed for the fourth time. The medium state corresponding to the detection signal of the first sensor 1 after the fourth change is liquid. At this time, the medium state corresponding to the detection signals output by the first sensor 1 and the second sensor 2 is liquid. However, the number of changes in the detection signals of the first sensor 1 and the second sensor 2 is different. Therefore, the sampling pump 4 continues to sample.

[0050] The medium changed from the third to the fourth time is the third medium air segment, and the medium after the fourth change is the fourth medium liquid segment. Until the fourth medium liquid segment reaches the second sensor 2, the detection signal of the first sensor 1 has not changed for the fifth time during this period. The medium state corresponding to the detection signals of the first sensor 1 and the second sensor 2 is liquid, and the detection signals of the first sensor 1 and the second sensor 2 have changed four times. Therefore, it can be concluded that the first medium from the first sensor 1 to the second sensor 2 is entirely liquid, without bubbles or air. The controller controls the sampling pump 4 to stop sampling and closes the control valve 5.

[0051] In this embodiment, the above-mentioned sampling unit was used to conduct a quantitative sampling experiment. The sample was 50 μL of pure water, and the experimental results are as follows:

[0052]

[0053] The experimental results above show that the maximum error in the 10 experiments was 1.1 μL, which meets the accuracy requirements of the standard sampling volume.

[0054] After the liquid sample is injected into the detection card, the detection module acquires the reaction image of the detection card, performs image processing and analysis, and outputs the analysis results. The detection module includes a processing unit and an analysis unit; the processing unit acquires the reaction image of the detection card, and the analysis unit performs binarization processing on the reaction image to obtain the detection result based on the processed reaction image.

[0055] In this embodiment, taking syphilis detection and analysis as an example, the reaction principle between the test card and the liquid sample is the TURBT detection principle; the test fluid containing syphilis reacts with the test card to obtain a colored agglomerate, as shown in the attached image. Figure 4 As shown; the processing unit detects and acquires the effective area of ​​the detection card and performs binarization processing, as shown in the attached figure. Figure 5 and attached Figure 6 As shown, the analysis unit obtains the detection results based on the diameter of the colored condensate in the processed reaction image. The detection results of the liquid samples in regions 1 to 4 are positive, while the detection results of the liquid samples in regions 5 and 6 are negative.

[0056] As attached Figure 1 As shown, in order to recover the tested liquid samples and avoid the spread of the virus, a virus detection and analysis device also includes a recovery module, which is used to seal and recover the tested test tubes and test cards after testing.

[0057] As attached Figure 1 As shown, in order to make a virus detection and analysis device more automated and improve its safety and convenience, the virus detection and analysis device also includes a pre-processing module, which is used to perform pre-sampling processing and fix the test tube containing the liquid sample.

[0058] Specifically, the preprocessing module includes an identification unit, a gripping unit, a scanning unit, and a cap removal unit;

[0059] The identification unit is used to identify whether there is a test tube in the area to be inspected;

[0060] When a test tube is detected in the area to be inspected, the gripping unit is controlled to grip the test tube.

[0061] The scanning unit is used to scan the identification code on the test tube; the test tube usually has a barcode or QR code affixed to it, which is used to record the patient information to which the test sample belongs; by obtaining the patient information through the scanning unit, it is convenient to match and statistically analyze the test results of the sample;

[0062] Since the orientation of the identification code on the test tube is not fixed, the gripping unit is also used to rotate the test tube to facilitate the scanning unit to successfully scan the identification code on the test tube.

[0063] The cap removal unit is used to remove the cap from the gripped test tube. It also re-capsulates the test tube after sampling. To prevent contamination or leakage of liquid samples before testing, test tubes are typically sealed with caps. Therefore, removing the cap after scanning the identification code prevents liquid sample leakage during gripping or rotation. After sampling, the cap removal unit also re-seals the cap back onto the test tube to prevent liquid sample leakage and contamination.

[0064] If there are multiple test tubes to be tested in the area to be inspected, the cap removal unit reseals the cap of the previous test tube onto the test tube, and the clamping unit puts the sampled test tube into the recovery module and clamps the next test tube in the area to be inspected to start the scanning and cap removal operation.

[0065] As attached Figure 3 As shown, to ensure that the test results of multiple liquid samples do not interfere with each other, the sample addition module also includes a cleaning unit, which is used to clean the sample addition unit. The cleaning unit includes a cleaning device 9, with a cleaning inlet at the upper end. The shape and size of the cleaning inlet are determined according to the sampling tube 3 to ensure that the sampling tube 3 can pass through the cleaning inlet smoothly, and the gap between the sampling tube 3 and the cleaning inlet does not exceed 1 mm. The other end of the cleaning device 9 is also connected to a waste liquid pipe 13, and a waste discharge pump 14 is installed on the waste liquid pipe 13. A cleaning pipe 15 is connected next to the cleaning inlet of the cleaning device 9, and the other end of the cleaning pipe 15 is connected to a cleaning liquid storage chamber 11. A cleaning pump 10 is also installed on the cleaning pipe 15. A negative pressure device 8 is also installed at the cleaning inlet of the cleaning device 9. The negative pressure device 8, the cleaning pump 10, and the waste discharge pump 14 are electrically connected to the controller.

[0066] As attached Figure 3 As shown, a cleaning sensor 12 electrically connected to the controller is also provided on the cleaning pipe 15. The cleaning sensor 12 is used to detect whether the cleaning pump 10 draws cleaning fluid into the cleaning device 9 to ensure that the cleaning process proceeds smoothly.

[0067] The cleaning process is as follows: Sampling tube 3 is inserted into cleaning device 9 through the cleaning inlet. The controller starts cleaning pump 10 to draw cleaning solution from cleaning solution storage chamber 11 into cleaning device 9. The cleaning solution washes the outer wall of sampling tube 3 at a relatively fast speed to clean the outer wall of sampling tube 3. After the outer wall is cleaned, waste pump 14 is started to draw out the cleaning solution in cleaning device 9. Cleaning pump 10 is started again to draw out cleaning solution into cleaning device 9. Sampling pump 4 is started and control valve 5 is opened. Sampling pump 4 draws cleaning solution from cleaning device 9 into sampling tube 3 to clean the inner wall of sampling tube 3. When the absorption time of cleaning solution is longer than the sampling time, the controller controls sampling pump 4 to discharge cleaning solution into cleaning device 9. Waste pump 14 is started to draw out cleaning solution from waste liquid pipe 13. Negative pressure device 8 is started to draw out the cleaning solution remaining on sampling tube 3.

[0068] After cleaning, the controller acquires the real-time detection signals from the first and second sensors respectively, and compares the real-time detection signals with the standard detection signals of the clean sampling tube 3 to determine whether the sampling tube 3 is clean.

[0069] As attached Figure 1 As shown, in order to ensure that the liquid sample fully contacts and reacts with the test card, the sample addition module also includes a shaking unit; the shaking unit is used to shake the test card paper into which the liquid sample is injected, and the shaking time and shaking frequency are adjusted according to the different viruses being detected; in this embodiment, taking syphilis detection and analysis as an example, the shaking frequency is 100 r / min and the shaking time is 8 min.

[0070] In the description of this specification, references to terms such as "one embodiment," "some embodiments," "example," "specific example," or "some examples," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of the invention. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples.

[0071] Although embodiments of the invention have been shown and described, those skilled in the art will understand that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the claims and their equivalents.

Claims

1. A viral detection assay device, characterized by, Includes a sample addition module and a detection module; The sample addition module is used to draw liquid samples from test tubes and inject the liquid samples onto the test card paper. The sample addition module includes a sampling unit, which includes a sampling pump (4), a sampling tube (3), a control valve (5), and a controller. A first sensor (1) and a second sensor (2) are also fixed outside the sampling tube (3). The distance between the first sensor (1) and the second sensor (2) on the sampling tube (3) is calculated based on the standard sampling volume and the diameter of the sampling tube (3). The controller is also used to record the time interval between two adjacent detection signal changes of the first sensor (1) or the second sensor (2), and can also calculate the length of the medium passing through the sampling tube (3) in a certain time interval based on the flow rate and speed parameters of the sampling pump (4) and the diameter of the sampling tube (3). The first sensor (1) and the second sensor (2) are used to detect the state of the medium in the sampling tube (3) at their respective positions. The state of the medium includes liquid, bubbles and air. The controller controls the sampling pump (4) and the control valve (5) to work according to the detection signals output by the first sensor (1) and the second sensor (2). The detection module is used to acquire reaction images of the detection paper and perform image processing and analysis, and output the analysis results.

2. The virus detection and analysis device as described in claim 1, characterized in that, Both the first sensor (1) and the second sensor (2) include photoelectric sensors, and the photoelectric sensors output corresponding detection signals according to the morphology of the medium inside the sampling tube (3); The sampling process for liquid samples is as follows: The controller controls the sampling pump (4) to start sampling. The first sensor (1) and the second sensor (2) read the medium morphology at the corresponding position on the sampling tube (3) and output detection signals respectively. The controller reads the detection signals output by the first sensor (1) and the second sensor (2) in real time, obtains the medium morphology at the corresponding position based on the detection signals, and counts the number of changes in the detection signals of the first sensor (1) and the second sensor (2) in real time. When the medium morphology at the corresponding position of the first sensor (1) and the second sensor (2) is liquid, and the number of changes in the detection signals of the first sensor (1) and the second sensor (2) is equal, the controller controls the sampling pump (4) to stop and closes the control valve (5). The process of injecting liquid samples into the detection card is as follows: the controller opens the control valve (5) and controls the sampling pump (4) to start draining the liquid. When the detection signal of the first sensor (1) changes once, the controller controls the sampling pump (4) to stop draining the liquid and the control valve (5) closes.

3. A virus detection and analysis device as described in claim 1 or 2, characterized in that, Both the first sensor (1) and the second sensor (2) also include a filter circuit. The filter circuit is used to filter out interference signals in the detection signal. A comparator is also connected between the first sensor (1), the second sensor (2) and the controller. The comparator is used to output a digital signal based on the detection signal obtained by the photoelectric sensor. The controller combines the digital signal and the filtered detection signal to control the operation of the sampling pump (4) and the control valve (5).

4. The virus detection and analysis device as described in claim 1, characterized in that, The sample dispensing module also includes a cleaning unit, which is used to clean the sample dispensing unit; The cleaning unit includes a cleaning device (9), one end of which is provided with a cleaning inlet, and the other end of which is connected to a waste liquid pipe (13). A waste discharge pump (14) is provided on the waste liquid pipe (13). A cleaning pipe (15) is connected next to the cleaning inlet of the cleaning device (9). The other end of the cleaning pipe (15) is connected to a cleaning liquid storage chamber (11). A cleaning pump (10) is also provided on the cleaning pipe (15). A negative pressure device (8) is also provided at the cleaning inlet of the cleaning device (9). The negative pressure device (8), the cleaning pump (10), and the waste discharge pump (14) are electrically connected to the controller.

5. The virus detection and analysis device as described in claim 4, characterized in that, The cleaning process is as follows: The sampling tube (3) is inserted into the cleaning device (9) through the cleaning inlet. The controller starts the cleaning pump (10) to draw the cleaning liquid from the cleaning liquid storage chamber (11) into the cleaning device (9) to clean the outer wall of the sampling tube (3). After the outer wall is cleaned, the waste pump (14) is started to draw out the cleaning liquid in the cleaning device (9). The cleaning pump (10) is started again to draw out the cleaning liquid into the cleaning device (9). The sampling pump (4) is started and draws the cleaning liquid from the cleaning device (9) into the sampling tube (3) to clean the inner wall of the sampling tube (3). When the absorption time of the cleaning liquid is greater than the sampling time, the controller controls the sampling pump (4) to discharge the cleaning liquid to the cleaning device (9). The waste pump (14) is started to draw out the cleaning liquid from the waste liquid pipe (13). The negative pressure device (8) is started to draw out the cleaning liquid remaining on the sampling tube (3).

6. A virus detection and analysis device as described in claim 1, 2, 4 or 5, characterized in that, The sample dispensing module also includes a shaking unit; the shaking unit is used to shake the test card containing the injected liquid sample.

7. A virus detection and analysis device as described in claim 1, 2, 4 or 5, characterized in that, The detection module includes a processing unit and an analysis unit; the processing unit is used to acquire the reaction image of the detection card, and the analysis unit is used to perform binarization processing on the reaction image and obtain the detection result based on the processed reaction image.

8. A virus detection and analysis device as described in claim 1, 2, 4 or 5, characterized in that, It also includes a pretreatment module, which is used to perform pretreatment of samples and fix the test tubes containing liquid samples. The preprocessing module includes a recognition unit, a gripping unit, and a scanning unit; The identification unit is used to identify whether there is a test tube in the area to be inspected; The gripping unit is used to grip and rotate the test tube; The scanning unit is used to scan the identification code on the test tube.

9. The virus detection and analysis device as described in claim 8, characterized in that, The pretreatment module also includes a cap removal unit, which is used to remove the caps from the gripped test tubes and to seal the test tubes after the sampling process is completed.

10. A virus detection and analysis device as described in claim 1, 2, 4, 5 or 9, characterized in that, It also includes a recycling module; the recycling module is used to seal and recycle the test tubes and test cards after testing.

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