A long-acting mosquito repellent emulsion containing soapberry nanocomposites and a preparation method thereof

Abstract

The application discloses a long-acting mosquito repellent emulsion containing sapindus saponin nano-complex and a preparation method thereof, and belongs to the mosquito repellent field. The application firstly combines and compounding three plant essential oils, i.e. citronella oil, peppermint oil and leaf oil of wormwood, to obtain mosquito repellent essential oil with better mosquito repellent effect, then prepares sapindus saponin nano-complex by using a reverse solvent precipitation method, and finally places the nano-complex in the mosquito repellent essential oil / water medium and prepares a pure natural long-acting mosquito repellent emulsion by high-speed homogenization emulsification. The sapindus saponin nano-complex is innovatively used to emulsify and embed the mosquito repellent essential oil, so that the mosquito repellent effect and effective protection time on mosquitoes are further improved, the prepared mosquito repellent emulsion does not contain chemical components such as diethyltoluamide and mosquito repellent ester, the mosquito repellent emulsion can safely and mildly repel mosquitoes, and the mosquito repellent emulsion will not cause resistance of mosquitoes.

Description

Technical Field

[0001] This invention belongs to the field of mosquito repellents, specifically relating to a long-lasting mosquito repellent emulsion containing Sapindus mukorossi nanocomposite and its preparation method. Background Technology

[0002] Mosquitoes belong to the family Culicidae in the order Diptera. They are diverse in species and reproduce rapidly, with mosquito populations increasing dramatically every summer, causing significant disruption to human life. Mosquito bites not only cause allergies and itching but can also transmit various serious diseases, such as malaria, dengue fever, Japanese encephalitis, lymphatic filariasis, yellow fever, and equine encephalitis, seriously threatening human health. Mosquito repellent products are essential for summer use. Currently, commonly used mosquito repellents mainly contain DEET and picaridin; however, these products have certain toxic side effects on humans, and prolonged use can lead to mosquito resistance, reducing their effectiveness and causing environmental pollution.

[0003] Many plants in nature possess mosquito-repellent properties, such as mosquito-repellent grass, lavender, eucalyptus leaves, lemongrass, and mugwort. The essential oils they contain have high mosquito-repellent activity and are safe and non-toxic to humans, unlikely to induce resistance in mosquitoes, and easily degraded. Currently, various essential oils have been extracted from these mosquito-repellent plants for use, but their direct application often suffers from low repellency and short duration of protection. Based on this, researchers have combined and blended multiple essential oils to create various blended essential oils with better mosquito-repellent effects, improving their repellency and effective protection time to some extent. However, the effective protection time of these blended essential oils obtained using this method is generally less than 6 hours, still insufficient to provide long-term effective mosquito repellency. Therefore, it is still necessary to develop effective technologies to improve the mosquito-repellent effect and protection time of plant essential oils to meet human mosquito control needs.

[0004] Oil-in-water nanoemulsions possess high stability, strong physicochemical properties, good controlled-release properties, and excellent bioactivity. They can be used for the encapsulation and sustained release of active ingredients such as plant essential oils. In particular, oil-in-water nanoemulsions stabilized with solid particles exhibit even better stability, superior encapsulation efficiency, and lower electrolysis rates, thus providing better protection for active small molecules to a certain extent. The solid particles used to stabilize oil-in-water nanoemulsions are generally divided into two categories: inorganic particles and organic particles. Inorganic particles mainly include modified silica, titanium dioxide, and clay particles, while organic particles mainly include proteins, polysaccharides, nanocellulose, and bioactive small molecules such as flavonoids and phytosterols. Compared to inorganic particles, organic particles are biodegradable, biocompatible, sustainable, and non-toxic, making them more suitable for preparing green mosquito repellent products. Summary of the Invention

[0005] The purpose of this invention is to provide a long-lasting mosquito repellent emulsion containing Sapindus mukorossi nanocomplex and its preparation method. By emulsifying and encapsulating mosquito repellent essential oil using a Sapindus mukorossi seed kernel prolysin / Sapindus mukorossi polysaccharide nanocomplex, the resulting mosquito repellent emulsion does not produce toxic side effects on the human body, has a significant and long-lasting mosquito repellent effect, and does not induce mosquito resistance, thus meeting people's needs for mosquito repellent in summer.

[0006] The objective of this invention is achieved through the following technical solutions.

[0007] First, a mosquito-repellent essential oil with good mosquito-repellent effect is obtained by blending three pure natural plant essential oils: citronella oil, peppermint oil, and mugwort oil. Then, a soapberry seed kernel alcohol-soluble protein / soapberry polysaccharide nanocomposite is prepared by antisolvent precipitation method. Finally, the nanocomposite is dispersed in the mosquito-repellent essential oil / water medium and emulsified by high-speed homogenization to obtain a pure natural long-lasting mosquito-repellent emulsion.

[0008] Using the above technical solution, three pure natural plant essential oils—citronella oil, peppermint oil, and mugwort oil—are first combined and compounded. Relying on the synergistic effect between different plant essential oils, the mosquito repellent efficiency and effective protection time of the mosquito repellent essential oil can be effectively improved. Then, the mosquito repellent essential oil is used as the core material and emulsified and encapsulated in a mosquito repellent essential oil / water medium by a Sapindus mukorossi seed kernel alcohol-soluble protein / Sapindus mukorossi polysaccharide nanocomposite. This not only slows down the evaporation rate of the mosquito repellent essential oil, but also the components contained in the Sapindus mukorossi nanocomposite help to improve its repellency effect on mosquitoes, thus obtaining a long-lasting mosquito repellent emulsion containing Sapindus mukorossi nanocomposite.

[0009] Specifically, a method for preparing a long-lasting mosquito repellent emulsion containing Sapindus mukorossi nanocomplex includes the following steps:

[0010] S1. Plant essential oil extraction: Lemongrass, peppermint leaves, and artemisia leaves were freeze-dried and pulverized, passed through a 30-mesh sieve, and 800g of each plant powder was weighed into a 5L extraction vessel and loaded into a supercritical CO2 extraction device. Extraction was carried out under the following conditions: temperature, pressure, time, and CO2 flow rate of 38-42℃, 24-26Mpa, 80-100min, and 23-27L / h, respectively. The obtained products were refrigerated at 4℃ for 12-15h, filtered to remove impurities, and lemongrass oil, peppermint oil, and artemisia oil were obtained, respectively.

[0011] S2. Mosquito repellent essential oil blend: Combine citronella oil, peppermint oil and mugwort oil in a volume ratio of 1-3:1-3:1, stir at 110-130 r / min for 2-2.5 h to obtain a mosquito repellent essential oil with better effect.

[0012] S3. Extraction of Sapindus mukorossi seed kernel alcohol-soluble protein: Dry and pulverize Sapindus mukorossi seeds, pass through a 30-mesh sieve, disperse in 70-90% ethanol at a mass content of 10%, stir at 110-130 r / min for 2-4 h, centrifuge at 8000-8500 r / min for 20-25 min to obtain the supernatant, concentrate by rotary evaporation at 38-42℃ and 35-40 r / min for 10-15 min, and freeze-dry the obtained product to obtain Sapindus mukorossi seed kernel alcohol-soluble protein;

[0013] S4. Extraction of Sapindus mukorossi polysaccharides: Dry and pulverize the Sapindus mukorossi pericarp, pass it through a 30-mesh sieve, add deionized water at a material-to-liquid ratio of 1:10-30 g / mL, and place it in a 400-450W ultrasonic reactor.

[0014] Extract at 60–80℃ for 20–60 min, centrifuge at 4000–4500 r / min for 10–15 min, and collect the supernatant.

[0015] After being concentrated by rotary evaporation at 38–42℃ and 35–40 r / min for 10–15 min, the product was precipitated with anhydrous ethanol, allowed to stand at 4℃ for 10–12 h, centrifuged, washed with anhydrous ethanol, acetone, and anhydrous ether, and then freeze-dried to obtain Sapindus mukorossi polysaccharide.

[0016] S5. Preparation of nanocomposite: Sapindus mukorossi seed kernel alcohol-soluble protein was dissolved in 70-90% ethanol to obtain Sapindus mukorossi seed kernel alcohol-soluble protein solution. Sapindus mukorossi polysaccharide was dissolved in deionized water to obtain Sapindus mukorossi polysaccharide solution. The Sapindus mukorossi seed kernel alcohol-soluble protein solution was added dropwise to the Sapindus mukorossi polysaccharide solution under continuous stirring. The mass ratio of Sapindus mukorossi seed kernel alcohol-soluble protein to Sapindus mukorossi polysaccharide in the resulting mixed solution was 3-5:1, and the total mass of Sapindus mukorossi seed kernel alcohol-soluble protein and Sapindus mukorossi polysaccharide was 1-3% of the mass of the mixed solution. The mixture was stirred for 30-35 min. The mixed solution was concentrated by rotary evaporation at 38-42℃ and 35-40 r / min for 10-15 min and then freeze-dried to obtain Sapindus mukorossi seed kernel alcohol-soluble protein / Sapindus mukorossi polysaccharide nanocomposite.

[0017] S6. Preparation of mosquito repellent emulsion: Mix mosquito repellent essential oil and water in equal amounts to obtain an oil-water mixture. Add the above nanocomposite to the oil-water mixture at a mass content of 5-15%. Stir magnetically for 5-10 minutes. Then, use a high-speed homogenizer to shear at a speed of 10000-14000 r / min for 1-4 minutes to obtain a long-lasting mosquito repellent emulsion.

[0018] This invention employs the above-mentioned technical solution, combining pure natural plant essential oils and then emulsifying and encapsulating them with a Sapindus mukorossi seed kernel prolysin / Sapindus mukorossi polysaccharide nanocomposite to obtain a mosquito repellent emulsion. Compared with traditional mosquito repellent products, this invention has the following beneficial effects:

[0019] (1) This invention combines three kinds of plant essential oils with good mosquito repellent effect, which further improves the mosquito repellent efficiency and effective protection time of the essential oils, and the aroma of the essential oils is effectively improved.

[0020] (2) In this invention, the mosquito repellent essential oil is emulsified and encapsulated using a soapberry kernel alcohol-soluble protein / soapberry polysaccharide nanocomposite to control the evaporation rate of the essential oil. At the same time, soapberry polysaccharide and soapberry kernel protein also have a certain promoting effect on mosquito repellency.

[0021] (3) In the prior art, mosquito repellent products are usually prepared using chemical repellents such as DEET and IR3535 as core ingredients. These repellents have certain toxic effects on the human body and easily lead to resistance in mosquitoes. Mosquito repellents prepared using pure natural plant essential oils are popular because they are safe, non-toxic, do not easily develop resistance, and are easy to degrade. However, their mosquito repellent effect and effective protection time are difficult to guarantee. Compared with traditional mosquito repellent products, the mosquito repellent lotion prepared in this invention has significantly improved mosquito repellent effect and effective protection time, and has no toxic side effects on the human body, which can meet people's daily mosquito repellent needs. Detailed Implementation

[0022] The present invention will be further described in detail below with reference to the embodiments.

[0023] The endpoints and any values ​​of the ranges disclosed herein are not limited to the precise ranges or values, and these ranges or values ​​should be understood to include values ​​close to these ranges. For numerical ranges, the endpoint values ​​of the various ranges, the endpoint values ​​of the various ranges and individual point values, and individual point values ​​can be combined with each other to obtain one or more new numerical ranges, which should be considered as specifically disclosed in this invention.

[0024] Example 1

[0025] A long-lasting mosquito repellent emulsion containing Sapindus mukorossi nanocomposite is prepared according to the following steps:

[0026] S1. Plant essential oil extraction: Lemongrass, peppermint leaves, and mugwort leaves were freeze-dried, pulverized, and passed through a 30-mesh sieve. 800g of each plant powder was weighed into a 5L extraction vessel and loaded into a supercritical CO2 extraction device. Extraction was carried out under the following conditions: temperature, pressure, time, and CO2 flow rate of 40℃, 25MPa, 90min, and 25L / h, respectively. The resulting products were refrigerated at 4℃ for 12h, filtered to remove impurities, and lemongrass oil, peppermint oil, and mugwort oil were obtained, respectively.

[0027] S2. Mosquito repellent essential oil blend: Combine citronella oil, peppermint oil and mugwort oil in a volume ratio of 2:2:1 and stir at 120r / min for 2 hours to obtain a mosquito repellent essential oil with better effect.

[0028] S3. Extraction of Sapindus mukorossi kernel alcohol-soluble protein: Dry and pulverize Sapindus mukorossi kernels, pass them through a 30-mesh sieve, disperse them in 80% ethanol at a mass content of 10%, stir at 120 r / min for 3 h, centrifuge at 8000 r / min for 20 min to obtain the supernatant, and rotary evaporate at 40℃ and 35 r / min for 10 min. Freeze-dry the obtained product to obtain Sapindus mukorossi kernel alcohol-soluble protein.

[0029] S4. Extraction of Sapindus mukorossi polysaccharides: Dry and pulverize the pericarp of Sapindus mukorossi, pass it through a 30-mesh sieve, add deionized water at a material-to-liquid ratio of 1:20 g / mL, place it in a 400W ultrasonic reactor, extract at 70℃ for 40 min, centrifuge at 4000 r / min for 15 min, concentrate the supernatant by rotary evaporation, precipitate it with anhydrous ethanol, let it stand at 4℃ for 12 h, centrifuge it, wash the precipitate with anhydrous ethanol, acetone and anhydrous ether, and freeze-dry it to obtain Sapindus mukorossi polysaccharides.

[0030] S5. Preparation of the nanocomposite: Sapindus mukorossi seed kernel alcohol-soluble protein was dissolved in 80% ethanol to obtain a Sapindus mukorossi seed kernel alcohol-soluble protein solution. Sapindus mukorossi polysaccharide was dissolved in deionized water to obtain a Sapindus mukorossi polysaccharide solution. Under continuous stirring, the Sapindus mukorossi seed kernel alcohol-soluble protein solution was added dropwise to the Sapindus mukorossi polysaccharide solution, controlling the mass ratio of Sapindus mukorossi seed kernel alcohol-soluble protein to Sapindus mukorossi polysaccharide in the resulting mixed solution to be 4:1. The total mass of Sapindus mukorossi seed kernel alcohol-soluble protein and Sapindus mukorossi polysaccharide was 2% of the mass of the mixed solution. Stirring was continued for 30 min. The mixed solution was then rotary evaporated at 40℃ and 35 r / min for 10 min, followed by freeze-drying to obtain the Sapindus mukorossi seed kernel alcohol-soluble protein / Sapindus mukorossi polysaccharide nanocomposite.

[0031] S6. Preparation of mosquito repellent lotion: Mix mosquito repellent essential oil and water in equal amounts to obtain an oil-water mixture solution. Add the above nanocomposite to the oil-water mixture solution at a mass content of 10%. Stir magnetically for 5 minutes. Shear at 12000 r / min for 2 minutes using a high-speed homogenizer to obtain a pure natural long-lasting mosquito repellent lotion.

[0032] Example 2

[0033] A long-lasting mosquito repellent emulsion containing Sapindus mukorossi nanocomposite is prepared according to the following steps:

[0034] S1. Plant essential oil extraction: Lemongrass, peppermint leaves, and mugwort leaves were freeze-dried, pulverized, and passed through a 30-mesh sieve. 800g of each plant powder was weighed into a 5L extraction vessel and loaded into a supercritical CO2 extraction device. Extraction was carried out under the following conditions: temperature, pressure, time, and CO2 flow rate of 40℃, 25MPa, 90min, and 25L / h, respectively. The resulting products were refrigerated at 4℃ for 12h, filtered to remove impurities, and lemongrass oil, peppermint oil, and mugwort oil were obtained, respectively.

[0035] S2. Mosquito repellent essential oil blend: Combine citronella oil, peppermint oil and mugwort oil in a volume ratio of 1:1:1 and stir at 120r / min for 2 hours to obtain a mosquito repellent essential oil with better effect.

[0036] S3. Extraction of Sapindus mukorossi kernel alcohol-soluble protein: Dry and pulverize Sapindus mukorossi kernels, pass them through a 30-mesh sieve, disperse them in 70% ethanol at a mass content of 10%, stir at 120 r / min for 2 h, centrifuge at 8000 r / min for 20 min to obtain the supernatant, and rotary evaporate at 40℃ and 35 r / min for 10 min. Freeze-dry the obtained product to obtain Sapindus mukorossi kernel alcohol-soluble protein.

[0037] S4. Extraction of Sapindus mukorossi polysaccharides: Dry and pulverize the pericarp of Sapindus mukorossi, pass it through a 30-mesh sieve, add deionized water at a material-to-liquid ratio of 1:10 g / mL, place it in a 400W ultrasonic reactor, extract at 60℃ for 20 min, centrifuge at 4000 r / min for 15 min, concentrate the supernatant by rotary evaporation, precipitate with anhydrous ethanol, let stand overnight at 4℃, centrifuge, wash the precipitate with anhydrous ethanol, acetone, and anhydrous ether, and freeze-dry to obtain Sapindus mukorossi polysaccharides.

[0038] S5. Preparation of the nanocomposite: Sapindus mukorossi seed kernel alcohol-soluble protein was dissolved in 70% ethanol to obtain a Sapindus mukorossi seed kernel alcohol-soluble protein solution. Sapindus mukorossi polysaccharide was dissolved in deionized water to obtain a Sapindus mukorossi polysaccharide solution. Under continuous stirring, the Sapindus mukorossi seed kernel alcohol-soluble protein solution was added dropwise to the Sapindus mukorossi polysaccharide solution, controlling the mass product ratio of Sapindus mukorossi seed kernel alcohol-soluble protein to Sapindus mukorossi polysaccharide in the resulting mixed solution to be 3:1, and the total mass of Sapindus mukorossi seed kernel alcohol-soluble protein and Sapindus mukorossi polysaccharide to be 1% of the mass of the mixed solution. Stirring was continued for 30 min. The mixed solution was then rotary evaporated at 40℃ and 35 r / min for 10 min, followed by freeze-drying to obtain the Sapindus mukorossi seed kernel alcohol-soluble protein / Sapindus mukorossi polysaccharide nanocomposite.

[0039] S6. Preparation of mosquito repellent lotion: Mix mosquito repellent essential oil and water in equal amounts to obtain an oil-water mixture solution. Add the above nanocomposite to the oil-water mixture solution at a mass content of 5%. Stir magnetically for 5 minutes. Shear at 10000 r / min for 1 minute using a high-speed homogenizer to obtain a pure natural long-lasting mosquito repellent lotion.

[0040] Example 3

[0041] A long-lasting mosquito repellent emulsion containing Sapindus mukorossi nanocomposite is prepared according to the following steps:

[0042] S1. Plant essential oil extraction: Lemongrass, peppermint leaves, and mugwort leaves were freeze-dried, pulverized, and passed through a 30-mesh sieve. 800g of each plant powder was weighed into a 5L extraction vessel and loaded into a supercritical CO2 extraction device. Extraction was carried out under the following conditions: temperature, pressure, time, and CO2 flow rate of 40℃, 25MPa, 90min, and 25L / h, respectively. The resulting products were refrigerated at 4℃ for 12h, filtered to remove impurities, and lemongrass oil, peppermint oil, and mugwort oil were obtained, respectively.

[0043] S2. Mosquito repellent essential oil blend: Combine citronella oil, peppermint oil and mugwort oil in a volume ratio of 3:3:1, stir at 120r / min for 2 hours to obtain a mosquito repellent essential oil with better effect.

[0044] S3. Extraction of Sapindus mukorossi kernel alcohol-soluble protein: Dry and pulverize Sapindus mukorossi kernels, pass them through a 30-mesh sieve, disperse them in 90% ethanol at a mass content of 10%, stir at 120 r / min for 4 h, centrifuge at 8000 r / min for 20 min to obtain the supernatant, and rotary evaporate at 40℃ and 35 r / min for 10 min. Freeze-dry the obtained product to obtain Sapindus mukorossi kernel alcohol-soluble protein.

[0045] S4. Extraction of Sapindus mukorossi polysaccharides: Dry and pulverize the pericarp of Sapindus mukorossi, pass it through a 30-mesh sieve, add deionized water at a material-to-liquid ratio of 1:30 g / mL, place it in a 400W ultrasonic reactor and extract at 80℃ for 60 min, centrifuge at 4000 r / min for 15 min, concentrate the supernatant by rotary evaporation and precipitate it with anhydrous ethanol, let it stand overnight at 4℃, centrifuge it and wash the precipitate with anhydrous ethanol, acetone and anhydrous ether, and freeze-dry it to obtain Sapindus mukorossi polysaccharides.

[0046] S5. Preparation of the nanocomposite: Sapindus mukorossi seed kernel alcohol-soluble protein was dissolved in 90% ethanol to obtain a Sapindus mukorossi seed kernel alcohol-soluble protein solution. Sapindus mukorossi polysaccharide was dissolved in deionized water to obtain a Sapindus mukorossi polysaccharide solution. Under continuous stirring, the Sapindus mukorossi seed kernel alcohol-soluble protein solution was added dropwise to the Sapindus mukorossi polysaccharide solution, controlling the mass-to-volume ratio of Sapindus mukorossi seed kernel alcohol-soluble protein to Sapindus mukorossi polysaccharide in the resulting mixed solution to be 5:1, and the total mass of Sapindus mukorossi seed kernel alcohol-soluble protein and Sapindus mukorossi polysaccharide to be 3% of the total mass of the mixed solution. Stirring was continued for 30 min. The mixed solution was then rotary evaporated at 40℃ and 35 r / min for 10 min, followed by freeze-drying to obtain the Sapindus mukorossi seed kernel alcohol-soluble protein / Sapindus mukorossi polysaccharide nanocomposite.

[0047] S6. Preparation of mosquito repellent lotion: Mix mosquito repellent essential oil and water in equal amounts to obtain an oil-water mixture solution. Add the above nanocomposite to the oil-water mixture solution at a mass content of 15%. Stir magnetically for 5 minutes. Shear at 14000 r / min for 4 minutes using a high-speed homogenizer to obtain a pure natural long-lasting mosquito repellent lotion.

[0048] Comparative Example 1

[0049] A mosquito repellent lotion containing a Sapindus mukorossi nanocomposite differs from Example 1 in that it does not contain peppermint oil or Artemisia argyi oil.

[0050] Comparative Example 2

[0051] A mosquito repellent lotion containing a Sapindus mukorossi nanocomposite differs from Example 1 in that it does not contain citronella oil or Artemisia argyi oil.

[0052] Comparative Example 3

[0053] A mosquito repellent lotion containing a Sapindus mukorossi nanocomposite differs from Example 1 in that it does not contain citronella oil or peppermint oil.

[0054] Comparative Example 4

[0055] A pure natural mosquito repellent essential oil, which differs from Example 1 in that it does not contain Sapindus mukorossi seed kernel alcohol-soluble protein, Sapindus mukorossi polysaccharide and water.

[0056] Comparative Example 5

[0057] A mosquito repellent lotion, which differs from Example 1 in that the Sapindus mukorossi seed kernel prolysin and Sapindus mukorossi polysaccharide in the mosquito repellent lotion are replaced with wheat prolysin and pectin.

[0058] The mosquito repellent emulsions prepared in Examples 1 to 3 and Comparative Examples 1 to 5 were used to test mosquito repellency efficiency and effective protection time. The mosquito repellency efficiency test procedure was as follows: Two mosquito cages were connected by an plexiglass tube, with a movable baffle in the center of the passage. One hundred female Culex pipiens pallens were blown in from the left side, and an appropriate amount of the test sample was placed on the left side. After 60 minutes, the passage was cut off, and the number of mosquitoes and the number knocked down in both chambers were observed to calculate the mosquito repellency efficiency. The effective protection time test procedure followed GB / T 139179-2009 "Indoor Efficacy Tests and Evaluation of Pesticide Registration Sanitary Insecticides Part 9: Repellents". The test results are shown in Table 1.

[0059] Table 1. Test results of mosquito repellency efficiency and effective protection time.

[0060]

[0061]

[0062] As shown in Table 1, the mosquito repellent emulsions prepared in Examples 1 to 3 all achieved a 100% repellency rate against mosquitoes, with effective protection times of 16 hours, 14.5 hours, and 15.5 hours, respectively, which are higher than those prepared in Comparative Examples 1 to 3. This indicates that the combination and compounding of plant essential oils helps to improve their mosquito repellency duration. However, the mosquito repellent essential oil prepared in Comparative Example 4 showed significantly lower mosquito repellency and effective protection time compared to the mosquito repellent emulsions prepared in Examples 1 to 3. This indicates that the emulsification and encapsulation of the Sapindus mukorossi seed kernel prolysin / Sapindus mukorossi polysaccharide nanocomposite significantly improved the mosquito repellency and effective protection duration of the essential oil. Comparative Example 5 used a wheat prolysin / pectin nanocomposite emulsification and encapsulation of the mosquito repellent essential oil, and its mosquito repellency and effective protection duration were lower than those prepared in Examples 1 to 3. This indicates that Sapindus mukorossi seed kernel prolysin and Sapindus mukorossi polysaccharide play a certain role in improving mosquito repellency.

[0063] The preferred embodiments of the present invention have been described in detail above; however, the present invention is not limited thereto. Within the scope of the inventive concept, various simple modifications can be made to the technical solutions of the present invention, including combinations of various technical features in any other suitable manner. These simple modifications and combinations should also be considered as the content disclosed in the present invention and are all within the protection scope of the present invention.

Claims

1. A long-lasting mosquito repellent lotion containing a Sapindus mukorossi nanocomposite, characterized in that, The mosquito repellent lotion is prepared from Sapindus mukorossi seed kernel alcohol-soluble protein / Sapindus mukorossi polysaccharide nanocomposite, mosquito repellent essential oil and water. The mass ratio of the mosquito repellent essential oil to water is 1:1, and the mass of the Sapindus mukorossi seed kernel alcohol-soluble protein / Sapindus mukorossi polysaccharide nanocomposite is 5-15% of the total mass of the mosquito repellent essential oil and water. The mosquito repellent essential oil is a compound of lemongrass oil, peppermint oil and mugwort oil in a volume ratio of 1~3:1~3:

1. The preparation method of lemongrass oil, peppermint oil and mugwort oil is as follows: lemongrass, peppermint leaves and mugwort leaves are freeze-dried and pulverized. Each plant powder is weighed and placed in an extraction vessel, loaded into a supercritical CO2 extraction device for extraction. The obtained products are refrigerated and filtered to remove impurities, and lemongrass oil, peppermint oil and mugwort oil are obtained respectively. The Sapindus mukorossi kernel alcohol-soluble protein / Sapindus mukorossi polysaccharide nanocomposite is prepared by the following method: 1) Sapindus mukorossi kernels are dried and pulverized, and the supernatant is obtained by ethanol extraction. The supernatant is then concentrated by rotary evaporation and freeze-dried to obtain Sapindus mukorossi kernel alcohol-soluble protein; 2) Sapindus mukorossi pericarp is dried and pulverized, and the supernatant is obtained by water extraction. The supernatant is then concentrated by rotary evaporation and precipitated with anhydrous ethanol. The precipitate is then washed and freeze-dried to obtain Sapindus mukorossi polysaccharide; 3) The ethanol solution of Sapindus mukorossi kernel alcohol-soluble protein is added to the aqueous solution of Sapindus mukorossi polysaccharide to obtain a mixed solution. The mass ratio of Sapindus mukorossi kernel alcohol-soluble protein to Sapindus mukorossi polysaccharide in the mixed solution is 3~5:1, and the total mass of Sapindus mukorossi kernel alcohol-soluble protein and Sapindus mukorossi polysaccharide is 1~3% of the mass of the mixed solution. After stirring evenly, the solution is concentrated by rotary evaporation and freeze-dried to obtain the Sapindus mukorossi kernel alcohol-soluble protein / Sapindus mukorossi polysaccharide nanocomposite.

2. The preparation method of a long-lasting mosquito repellent emulsion containing Sapindus mukorossi nanocomposite as described in claim 1, characterized in that, Includes the following steps: S1. Plant essential oil extraction: Lemongrass, peppermint leaves and mugwort leaves were freeze-dried and pulverized. Each plant powder was weighed and placed in an extraction vessel, loaded into a supercritical CO2 extraction device for extraction. The obtained products were refrigerated and filtered to remove impurities, and lemongrass oil, peppermint oil and mugwort oil were obtained respectively. S2. Mosquito repellent essential oil blend: Mix citronella oil, peppermint oil and artemisia oil and stir well to obtain mosquito repellent essential oil; S3. Extraction of Sapindus mukorossi seed kernel alcohol-soluble protein: Dry and pulverize Sapindus mukorossi seeds, disperse them in 70-90% ethanol at 10% by mass, stir at 110-130 r / min for 2-4 h, centrifuge at 8000-8500 r / min for 20-25 min to obtain the supernatant, concentrate by rotary evaporation and freeze dry to obtain Sapindus mukorossi seed kernel alcohol-soluble protein; S4. Extraction of Sapindus mukorossi polysaccharides: Dry and crush the pericarp of Sapindus mukorossi, add water at a material-to-liquid ratio of 1:10~30 g / mL, place in a 400~450 W ultrasonic reactor, extract at 60~80℃ for 20~60 min, centrifuge at 4000~4500 r / min for 10~15 min, concentrate the supernatant by rotary evaporation, precipitate with anhydrous ethanol, let stand at 4℃ for 10~12 h, centrifuge, wash the precipitate, and freeze-dry to obtain Sapindus mukorossi polysaccharides; S5. Preparation of Sapindus mukorossi seed kernel alcohol-soluble protein / Sapindus mukorossi polysaccharide nanocomposite: Sapindus mukorossi seed kernel alcohol-soluble protein was dissolved in 70-90% ethanol to obtain Sapindus mukorossi seed kernel alcohol-soluble protein solution, and Sapindus mukorossi polysaccharide was dissolved in deionized water to obtain Sapindus mukorossi polysaccharide solution. Under continuous stirring, the Sapindus mukorossi seed kernel alcohol-soluble protein solution was added dropwise to the Sapindus mukorossi polysaccharide solution to obtain a mixed solution. The mixture was stirred for 30-35 min, concentrated by rotary evaporation, and freeze-dried to obtain the Sapindus mukorossi seed kernel alcohol-soluble protein / Sapindus mukorossi polysaccharide nanocomposite. S6. Preparation of mosquito repellent emulsion: Mix equal amounts of mosquito repellent essential oil and water to obtain an oil-water mixture. Add the soapberry seed kernel alcohol-soluble protein / soapberry polysaccharide nanocomposite to the oil-water mixture, stir magnetically until uniform, and then homogenize and emulsify to obtain a long-lasting mosquito repellent emulsion.

3. The method for preparing a long-lasting mosquito repellent emulsion containing Sapindus mukorossi nanocomposite according to claim 2, characterized in that, In step S1, the temperature, pressure, time, and CO2 flow rate of the extraction process are 38~42℃, 24~26 MPa, 80~100 min, and 23~27 L / h, respectively.

4. In the preparation method of a long-lasting mosquito repellent emulsion containing Sapindus mukorossi nanocomposite according to claim 2, in step S2, the stirring is carried out at a speed of 110~130 r / min for 2~2.5 h.

5. In the preparation method of a long-lasting mosquito repellent emulsion containing Sapindus mukorossi nanocomposite according to claim 2, in step S4, the precipitate is washed with anhydrous ethanol, acetone, and anhydrous diethyl ether.

6. In the preparation method of a long-lasting mosquito repellent emulsion containing Sapindus mukorossi nanocomposite according to claim 2, in step S3, S4 or S5, the temperature, rotation speed and time of the rotary evaporation concentration are: 38~42℃, 35~40 r / min and 10~15min.

7. The method for preparing a long-lasting mosquito repellent emulsion containing Sapindus mukorossi nanocomposite according to claim 2, wherein in step S6, the magnetic stirring time is 5-10 min, and the homogenization emulsification is performed by using a high-speed homogenizer at a speed of 10000-14000 r / min for 1-4 min.

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