An in-vitro embryo retrieval device

Abstract

The application relates to the technical field of embryo retrieval, and discloses an embryo in-vitro retrieval device which comprises a gas film type embryo retrieval structure and a piston type gas guiding structure and is arranged in the interior of a main longitudinal reciprocating cavity, the interior of the device is provided with a main piston which can move axially along the main longitudinal reciprocating cavity and can reduce the air pressure above a sheet-shaped elastic gas film and increase the air pressure in the peripheral area of a ring-shaped elastic gas film during movement. The embryo in-vitro retrieval device can use the adsorption capacity of gas under a negative pressure state to suck the in-vitro embryo into a specific area, and can use the circulation flow capacity of gas to close the opening end of the storage embryo area while sucking the embryo, so that the dripping phenomenon of the embryo during the transfer process is prevented, and the closed area can effectively block external pollutants and reduce the infection probability during the embryo retrieval process.

Description

Technical Field

[0001] This invention relates to the field of embryo retrieval technology, specifically to an in vitro embryo retrieval device. Background Technology

[0002] Currently, in in vitro research and clinical work on mammalian embryos, staff primarily use embryo pipettes to retrieve embryos. Embryo pipettes are divided into oral pipettes and manual pipettes. Traditional manual pipettes typically utilize Pasteur pipettes with silicone tips; embryos are retrieved by pressing the silicone tip. Traditional oral pipettes consist of a soft tube and a glass needle connected to the embryo. Due to their ease of use, speed, and control, oral pipettes are widely used.

[0003] For example, Chinese patent publication number CN206843460U discloses "An in vitro embryo retrieval device," whose main structure includes a silicone suction tip, an oral suction tip, a first connecting tube, a second connecting tube, an embryo glass needle, and a T-type three-way ball valve. The T-type three-way ball valve is connected to one end of the first connecting tube, one end of the second connecting tube, and the embryo glass needle, respectively. The other end of the first connecting tube is connected to the silicone suction tip, and the other end of the second connecting tube is connected to the oral suction tip. This in vitro embryo retrieval device combines oral and manual suction. During embryo retrieval or movement, the oral suction mode is used to draw the embryo into the embryo glass needle. When the embryo is retrieved to another culture dish, the manual suction mode is switched, and the embryo is placed in the culture dish by pressing the silicone suction tip. This avoids direct contact between harmful components such as bacteria or viruses from the operator's breath or saliva and the embryo culture medium, preventing contamination of the embryo and affecting its development.

[0004] For example, Chinese patent publication number "CN217202669U" discloses "An in vitro retrieval device for equine embryos," whose main structure includes a pressure bag. The output end of the pressure bag is fixedly connected to a rectangular three-way tube. The output ends on both sides of the rectangular three-way tube are respectively fixedly connected to a right connecting tube and a left connecting tube. The bottom ends of the right connecting tube and the left connecting tube are respectively equipped with a suction head and a glass needle. A rotating shaft is fixedly fitted on the inner side of the top of the rectangular three-way tube. This in vitro retrieval device for equine embryos, through the arrangement of the components inside the rectangular three-way tube, allows the position of the two sets of rotating plates to be adjusted by rotating the handle, thereby connecting the right connecting tube or the left connecting tube to the trachea respectively. This achieves the effect of step-by-step operation of the suction head and the glass needle, thus enabling the device to operate the suction head or the glass needle simultaneously. This solves the problem of existing devices requiring constant replacement of corresponding tools, which is cumbersome and inefficient. Furthermore, eliminating the operation of replacing tools can also reduce the probability of infection during embryo retrieval.

[0005] It is evident from the above description that the aforementioned in vitro embryo retrieval device first uses an oral suction head to aspirate the embryo from the culture dish when adsorbing the embryo. After the embryo is aspirated into the glass needle, the device switches to manual suction mode, pressing the suction head to place the embryo back into the culture dish. During the actual switching process, the change in the internal position of the T-type three-way ball valve causes a certain change in the space within the glass needle, resulting in a change in the longitudinal height of the embryo within the glass needle. This poses a risk of the embryo dripping along the suction port. Furthermore, since the suction end of the glass needle is open, it undoubtedly increases the degree of contamination of the embryo during the transfer process. Summary of the Invention

[0006] To address the shortcomings of existing technologies, this invention provides an in vitro embryo retrieval device. Utilizing the adsorption capacity of gas under negative pressure, it can draw in vitro embryos into a specific area. Simultaneously, the circulating gas flow can seal the opening of the embryo storage area, preventing embryo dripping during transfer. Furthermore, the sealed area effectively blocks external contaminants, reducing the probability of infection during embryo retrieval, thus solving the aforementioned technical problems.

[0007] To achieve the above objectives, the present invention provides the following technical solution: an in vitro embryo retrieval device, comprising a cylindrical hollow shell, a hollow rod structure disposed at the top of the cylindrical hollow shell, a component embedding groove disposed at the bottom of the cylindrical hollow shell, a main funnel-shaped cavity disposed at the top of the component embedding groove, a main longitudinal reciprocating cavity disposed at the top of the main funnel-shaped cavity, a gas flow channel disposed inside the cylindrical hollow shell and connecting the top of the main longitudinal reciprocating cavity and the bottom of the cylindrical hollow shell, and a shaft perforation disposed inside the hollow rod structure, and further comprising an air-film embryo retrieval device. The structure includes a sheet-like elastic gas membrane that deforms upward under negative pressure from above and draws the embryo into the deformed depression area, and an annular elastic gas membrane that deforms towards the center under gas pressure from the periphery and blocks the central area; and a piston-type gas guiding structure placed inside the main longitudinal reciprocating cavity, which contains a main piston that can move axially along the main longitudinal reciprocating cavity. During the movement, the piston reduces the gas pressure above the sheet-like elastic gas membrane and increases the gas pressure in the outer area of ​​the annular elastic gas membrane.

[0008] Preferably, the air-film embryo retrieval structure includes an embryo aspiration needle. The top of the embryo aspiration needle is provided with a cylindrical protrusion that can be inserted into a component embedding groove. A sealing ring is provided on the circumferential side of the cylindrical protrusion. An embryo aspiration hole communicating with its bottom is provided at the center of the embryo aspiration needle. A rod insertion groove with an open top and a bottom communicating with the embryo aspiration hole is provided inside the cylindrical protrusion. An annular gas compression space is provided outside the embryo aspiration needle near the bottom opening of the embryo aspiration hole. Multiple integrated insertion rod structures that can be inserted into the bottom of the gas flow channel are provided on the upper surface of the embryo aspiration needle outside the cylindrical protrusion. A first gas flow hole communicating with the space above the insertion rod structure and the annular gas compression space is provided inside the embryo aspiration needle. An annular elastic gas membrane is embedded in the embryo aspiration needle at the annular intersection of the embryo aspiration hole and the annular gas compression space. A sheet-like elastic gas membrane is embedded in the embryo aspiration needle above the annular elastic gas membrane inside the embryo aspiration hole.

[0009] Preferably, the sealing ring can be inserted into the component embedding groove, and after insertion, the sealing ring can seal the installation gap.

[0010] Preferably, both the annular elastic gas membrane and the sheet-like elastic gas membrane are made of elastic materials that are prone to deformation.

[0011] Preferably, the piston-type gas guiding structure includes a movable shaft that is inserted into the shaft through hole and can move axially along the shaft through hole. A main piston is installed at one end of the movable shaft located inside the main longitudinal reciprocating cavity. The bottom end of the main piston is provided with a main funnel-shaped boss structure that matches the main funnel-shaped cavity. The bottom end of the main funnel-shaped boss structure is provided with an integral cylindrical valve stem that can be inserted into the rod insertion slot. An array-shaped wing-shaped mounting plate is installed at the end of the movable shaft located above the hollow rod structure. The area between every two wing-shaped structures in the array-shaped wing-shaped mounting plate forms a fan-shaped notch. A pull rod is installed at the center of the top of the array-shaped wing-shaped mounting plate, and a pull plate is installed at the top of the pull rod.

[0012] Preferably, a sealing ring is fitted on the circumferential side of the main piston, and a sealing ring is embedded in the periphery of the shaft through a hole.

[0013] Preferably, it further includes a gas-confined reset structure, the gas-confined reset structure comprising an annular hollow shell, the center of which is provided with a central sleeve hole for fixedly fitting around the hollow rod structure; an annular gas flow space is provided at the bottom region of the annular solid area of ​​the annular hollow shell; three annular array-shaped secondary longitudinal reciprocating cavities are provided in the solid region above the annular gas flow space inside the annular hollow shell; a second gas flow hole is provided inside the annular hollow shell connecting the bottom end of the secondary longitudinal reciprocating cavities and the top end of the annular gas flow space; the annular hollow shell is further provided with a second gas flow hole connecting the bottom end of the secondary longitudinal reciprocating cavities and the top end of the annular gas flow space. A component mounting hole is provided in the solid area above the annular gas flow space. The bottom end of the component mounting hole is connected to the top end of the annular gas flow space, and the top end is open. A secondary piston that can move axially along the secondary longitudinal reciprocating cavity is installed inside each secondary longitudinal reciprocating cavity. A telescopic rod that penetrates the top structure of the annular hollow shell is installed on the upper end face of each secondary piston. The top end of each telescopic rod is fixedly connected to the bottom surface of a wing structure of the array-shaped wing mounting plate through a connecting plate. A main helical spring in a compressed state is sleeved on the rod body located inside the secondary longitudinal reciprocating cavity of each telescopic rod.

[0014] Preferably, a sealing ring is fitted onto the circumferential side of the auxiliary piston.

[0015] Preferably, it further includes an elastic gas flow control structure, which includes a cylindrical hollow body that can be fixedly placed inside a component mounting hole, and the cylindrical hollow body and the component mounting hole are filled with sealant at the mounting gap. The cylindrical hollow body has a longitudinal hollow cavity inside, and a third gas flow hole is provided at the bottom end of the longitudinal hollow cavity. An inverted secondary funnel-shaped cavity is provided at the bottom end of the cylindrical hollow body at the third gas flow hole, and the bottom end of the secondary funnel-shaped cavity communicates with the top end of the annular gas flow space. The top of the cylindrical hollow body has multiple fourth gas flow holes communicating with the longitudinal hollow cavity and the space above the cylindrical hollow body. A component that can be longitudinally placed inside the secondary funnel-shaped cavity... A movable, funnel-shaped boss structure that matches the external shape of the funnel-shaped cavity structure. A longitudinal rod is mounted at the top of the funnel-shaped boss structure, located inside the third gas flow hole and the longitudinal hollow cavity. The radius of the longitudinal rod is smaller than the structural radius of the third gas flow hole. A movable plate is mounted at the top of the longitudinal rod, located within the longitudinal hollow cavity. The structural radius of the longitudinal hollow cavity is larger than the structural radius of the movable plate. A compressed secondary helical spring is fitted around the rod inside the longitudinal hollow cavity. There is a movement gap between the upper surface of the movable plate and the top of the longitudinal hollow cavity. A push rod penetrating the central structure of the top of the cylindrical hollow body is mounted on the upper surface of the movable plate. A push plate is mounted at the top of the push rod.

[0016] Preferably, in the initial state, the elastic strength of the secondary helical spring is greater than the sum of the elastic strengths of the three main helical springs.

[0017] Compared with the prior art, the present invention provides an in vitro embryo retrieval device, which has the following beneficial effects:

[0018] This in vitro embryo retrieval device utilizes the adsorption capacity of gas under negative pressure to draw embryos into a specific area. Simultaneously, the circulating gas flow seals the opening of the embryo storage area, preventing embryos from dripping during transfer. The sealed area also effectively blocks external contaminants, reducing the probability of infection during embryo retrieval. Attached Figure Description

[0019] Figure 1 This is a schematic diagram of the full cross-section structure of the present invention;

[0020] Figure 2 This is a schematic diagram of the full cross-sectional structure of the air-film embryo retrieval structure in this invention;

[0021] Figure 3 This is a perspective view of the annular elastic gas film and the sheet-like elastic gas film in this invention;

[0022] Figure 4 This is a perspective view of the piston-type gas guiding structure in this invention;

[0023] Figure 5 This is a perspective view of the gas-confined reset structure in this invention;

[0024] Figure 6 This is a three-dimensional cross-sectional view of the gas-confined reset structure in this invention;

[0025] Figure 7 This is a perspective view of the elastic gas flow control structure in this invention;

[0026] Figure 8 This is a three-dimensional cross-sectional view of the elastic gas flow control structure in this invention.

[0027] The components include: 1. cylindrical hollow shell; 2. hollow rod structure; 3. component embedding groove; 4. main funnel-shaped cavity; 5. main longitudinal reciprocating cavity; 6. gas flow channel; 7. shaft perforation; 8. gas film embryo retrieval structure; 81. embryo aspiration needle; 82. cylindrical protrusion structure; 83. sealing ring; 84. embryo aspiration hole; 85. rod insertion groove; 86. annular gas compression space; 87. insertion rod structure; 88. first gas flow hole; 89. annular elastic gas film; 810. sheet-like elastic gas film; 9. piston-type gas guiding structure; 91. moving shaft; 92. main piston; 93. array-shaped wing-shaped mounting plate; 94. main funnel-shaped boss structure; 95. cylindrical valve stem; 96. fan-shaped notch; 9 7. Pull rod; 98. Pull plate; 10. Gas-confined reset structure; 101. Annular hollow shell; 102. Central sleeve hole; 103. Annular gas flow space; 104. Second gas flow hole; 105. Secondary longitudinal reciprocating cavity; 106. Secondary piston; 107. Telescopic rod; 108. Main helical spring; 109. Component mounting hole; 11. Elastic gas flow control structure; 111. Cylindrical hollow body; 112. Longitudinal hollow cavity; 113. Third gas flow hole; 114. Secondary funnel-shaped cavity; 115. Fourth gas flow hole; 116. Movable plate; 117. Push rod; 118. Secondary helical spring; 119. Secondary funnel-shaped boss structure; 1110. Push plate; 1111. Longitudinal rod. Detailed Implementation

[0028] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0029] Please see Figure 1 An in vitro embryo detection device includes a cylindrical hollow shell 1, a hollow rod structure 2 disposed at the top of the cylindrical hollow shell 1, a component embedding groove 3 disposed at the bottom of the cylindrical hollow shell 1, a main funnel-shaped cavity 4 disposed at the top of the component embedding groove 3, a main longitudinal reciprocating cavity 5 disposed at the top of the main funnel-shaped cavity 4, a gas flow channel 6 disposed inside the cylindrical hollow shell 1 and connecting the top of the main longitudinal reciprocating cavity 5 and the bottom of the cylindrical hollow shell 1, and a shaft through hole 7 disposed inside the hollow rod structure 2. During operation, gas can flow along the main longitudinal reciprocating cavity 5 and the gas flow channel 6, thereby achieving a gas circulation effect. Of course, the space of the main longitudinal reciprocating cavity 5 needs to be large enough, otherwise the compression or adsorption of gas will not meet the predetermined requirements.

[0030] To achieve the functions of embryo absorption, blocking, and expulsion, please refer to [link / reference needed]. Figure 1 , Figure 2 and Figure 3 A membrane-type embryo retrieval structure 8 is required, which includes a sheet-like elastic membrane 810 that deforms upward under negative pressure from above, drawing the embryo into the deformed recessed area, and an annular elastic membrane 89 that deforms towards the center under pressure from the periphery, blocking and closing the central area. When the air pressure above the sheet-like elastic membrane 810 drops to a negative pressure value below atmospheric pressure, the external atmospheric pressure will cause the sheet-like elastic membrane 810 to deform upward, thereby drawing the embryo into the recessed area of ​​the sheet-like elastic membrane 810, thus achieving embryo aspiration. Simultaneously, when the annular elastic membrane 89... When the periphery of the annular elastic gas membrane 89 is affected by high-pressure gas, the annular elastic gas membrane 89 will change towards its center until the annular opening of the annular elastic gas membrane 89 closes, thus achieving the blocking effect on the embryo. When the gas affecting the negative pressure and high pressure areas is compensated, the sheet-like elastic gas membrane 810 can return to its original position according to its own elasticity, thereby allowing the embryo to be discharged and sealing the opening of the embryo storage area, thus preventing the embryo from dripping during the transfer process. At the same time, the closed area can effectively block external contaminants and reduce the probability of infection during the embryo retrieval process.

[0031] For details regarding the specific structure of the aforementioned air-film embryo retrieval structure 8, please refer to [link / reference]. Figure 2 and Figure 3The device includes an embryo aspiration needle 81. The top of the embryo aspiration needle 81 has a cylindrical protrusion 82 that can be inserted into a component embedding groove 3. A sealing ring 83 is provided on the circumferential side of the cylindrical protrusion 82. To achieve detachable assembly and facilitate component replacement, the sealing ring 83 needs to be able to be inserted into the component embedding groove 3, and after insertion, the sealing ring 83 can seal the installation gap. The center of the embryo aspiration needle 81 has an embryo aspiration hole 84 communicating with its bottom. The interior of the cylindrical protrusion 82 has a rod insertion groove 85 with an open top and a bottom end communicating with the embryo aspiration hole 84. The interior of the embryo aspiration needle 81 has an annular gas compression space 86 located around the bottom opening of the embryo aspiration hole 84. The upper surface of the embryo aspiration needle 81 has multiple integrated structures located around the cylindrical protrusion 82, which can be correspondingly inserted... An insertion rod structure 87 is inserted into the bottom of the gas flow channel 6. The insertion rod structure 87 and the embryo aspiration needle 81 are provided with a first gas flow hole 88 that connects the space above the insertion rod structure 87 and the annular gas compression space 86. An annular elastic gas membrane 89 is embedded in the embryo aspiration hole 84 and the annular gas compression space 86 at the annular intersection of the embryo aspiration hole 84 and the annular gas compression space 86. A sheet-like elastic gas membrane 810 is embedded in the embryo aspiration hole 84 above the annular elastic gas membrane 89. In order to have the ability to deform and thus play different roles, both the annular elastic gas membrane 89 and the sheet-like elastic gas membrane 810 need to be made of elastic materials that are easy to deform. During operation, the bottom opening end of the embryo aspiration hole 84 needs to be immersed into the embryo. At the same time, the immersion depth of the embryo aspiration hole 84 needs to ensure that it does not come into contact with air during the aspiration process.

[0032] To achieve a circulating driving effect for the gas inside the structure, please refer to [link / reference needed]. Figure 1 and Figure 4 A piston-type gas guiding structure 9 is required, placed inside the main longitudinal reciprocating cavity 5. Inside the main longitudinal reciprocating cavity 5, there is a main piston 92 that can move axially. During the movement, the air pressure above the sheet-like elastic gas film 810 is reduced, while the gas pressure in the outer region of the annular elastic gas film 89 is increased. When the main piston 92 moves upward inside the main longitudinal reciprocating cavity 5, the space below the main piston 92 increases, which causes the air pressure above the sheet-like elastic gas film 810 to decrease. At the same time, the area above the main piston 92 decreases, allowing air above the main piston 92 to flow into the annular gas compression space 86 through the gas flow channel 6 and the first gas flow hole 88. This, in turn, increases the gas pressure in the outer region of the annular elastic gas film 89, thereby achieving a cyclic driving effect for the gas inside the structure. It has linkage and ease of operation.

[0033] For details regarding the specific structure of the piston-type gas guiding structure 9, please refer to [link / reference needed]. Figure 4 The system includes a movable shaft 91 that is inserted into the shaft through hole 7 and can move axially along the shaft through hole 7. A main piston 92 is mounted on one end of the movable shaft 91 located inside the main longitudinal reciprocating cavity 5. To prevent gas from flowing along the gaps in the movement of the components, a sealing ring is fitted onto the circumferential side of the main piston 92, and a sealing ring is embedded around the movable shaft 91 in the shaft through hole 7. The bottom end of the main piston 92 is provided with a main funnel-shaped boss structure 94 that matches the main funnel-shaped cavity 4. The bottom of the main funnel-shaped boss structure 94... The end is provided with a cylindrical valve stem 95 with an integrated structure that can be inserted into the rod insertion slot 85. The moving shaft 91 is equipped with an array-shaped wing-shaped mounting plate 93 at the end located above the hollow rod structure 2. The area between every two wing-shaped structures in the array-shaped wing-shaped mounting plate 93 forms a fan-shaped notch 96. A pull rod 97 is installed at the center of the top of the array-shaped wing-shaped mounting plate 93. A pull plate 98 is installed at the top of the pull rod 97. The presence of the main funnel-shaped boss structure 94 and the cylindrical valve stem 95 can improve the stability of gas flow.

[0034] To achieve stable embryo release capability, please refer to [link / reference]. Figure 1 , Figure 5 and Figure 6 A gas-confined reset structure 10 needs to be set up. For details on the specific structure of the gas-confined reset structure 10, please refer to [link / reference needed]. Figure 5 and Figure 6The system includes an annular hollow shell 101. The annular hollow shell 101 has a central sleeve hole 102 at its center, which can be fixedly fitted onto the periphery of the hollow rod structure 2. An annular gas flow space 103 is provided at the bottom of the annular solid region of the annular hollow shell 101. Three annular array-shaped secondary longitudinal reciprocating cavities 105 are provided inside the annular hollow shell 101 in the solid region above the annular gas flow space 103. A second gas flow hole 104 is provided inside the annular hollow shell 101, connecting the bottom end of the secondary longitudinal reciprocating cavity 105 to the top end of the annular gas flow space 103. A component mounting hole 109 is provided inside the annular hollow shell 101 in the solid region above the annular gas flow space 103. The bottom end of the component mounting hole 109 connects to the top end of the annular gas flow space 103, and the top end is open. Each secondary longitudinal reciprocating cavity 105 houses a secondary component that can move axially along the secondary longitudinal reciprocating cavity 105. To prevent gas from flowing along the movable gap, the piston 106 requires a sealing ring to be fitted on its circumferential side. Each secondary piston 106 has a telescopic rod 107 installed on its upper end face, which penetrates the top structure of the annular hollow shell 101. The top of each telescopic rod 107 is fixedly connected to the bottom surface of a wing-shaped structure of the array-shaped wing mounting plate 93 via a connecting plate. Each telescopic rod 107 has a main helical spring 108 in a compressed state fitted on its rod body located inside the secondary longitudinal reciprocating cavity 105. When the pull plate 98 is pulled upward, it will drive the telescopic rod 107 to move upward, thereby compressing the three main helical springs 108 again. The compressed main helical springs 108 are in an energy storage state. When the embryo is discharged, the pull plate 98 is released, and the energy storage state of the main helical springs 108 is released, resulting in a reset and elongation phenomenon. Because its reset process is elastic, it can discharge the embryo with a buffering effect and has a stable discharge capacity.

[0035] To control the rate of embryo release, please refer to [link / reference]. Figure 1 , Figure 7 and Figure 8 A flexible gas flow control structure 11 needs to be set up. For details on the specific structure of the flexible gas flow control structure 11, please refer to [link / reference needed]. Figure 7 and Figure 8The system includes a cylindrical hollow body 111 that can be fixedly placed inside a component mounting hole 109. The cylindrical hollow body 111 and the component mounting hole 109 have a sealant filling the mounting gap. The cylindrical hollow body 111 has a longitudinal hollow cavity 112 inside. A third gas flow hole 113 is provided at the bottom end of the longitudinal hollow cavity 112. An inverted secondary funnel-shaped cavity 114 is provided at the bottom end of the third gas flow hole 113, and the bottom end of the secondary funnel-shaped cavity 114 communicates with the top end of the annular gas flow space 103. The top of the cylindrical hollow body 111 has multiple fourth gas flow holes 114 that communicate with the longitudinal hollow cavity 112 and the space above the cylindrical hollow body 111. 15. Inside the secondary funnel-shaped cavity 114, a longitudinally movable secondary funnel-shaped boss structure 119 matching the structural shape of the secondary funnel-shaped cavity 114 is installed. A longitudinal rod 1111 located inside the third gas flow hole 113 and the longitudinal hollow cavity 112 is mounted on the top of the secondary funnel-shaped boss structure 119. The radius of the longitudinal rod 1111 is smaller than the structural radius of the third gas flow hole 113. A movable plate 116 is installed on the top of the longitudinal rod 1111 located in the longitudinal hollow cavity 112. The structural radius of the longitudinal hollow cavity 112 is larger than the structural radius of the movable plate 116. A compressed secondary helical spring 118 is sleeved around the rod 1111 located inside the longitudinal hollow cavity 112. To ensure effective control over gas sealing, the elastic strength of the secondary helical spring 118 must be greater than the sum of the elastic strengths of the three main helical springs 108 in the initial state. A movement gap exists between the upper end face of the movable plate 116 and the top of the longitudinal hollow cavity 112. A push rod 117, penetrating the central structure of the top of the cylindrical hollow body 111, is installed on the upper end face of the movable plate 116. A push plate 1110 is installed at the top of the push rod 117. During embryo suction, downward pressure is applied to the push plate 1110, creating a gap between the secondary funnel-shaped boss structure 119 and the conical surface of the secondary funnel-shaped cavity 114. At this time, external gas can enter the annular gas flow through the fourth gas flow hole 115 and this gap. Inside the dynamic space 103, a high-pressure zone is formed. After the embryo is completely sucked in, the pressure on the push plate 1110 is released. Under the action of the secondary helical spring 118, the gap between the secondary funnel-shaped boss structure 119 and the conical surface of the secondary funnel-shaped cavity 114 is closed, thus sealing the high-pressure gas inside the annular gas flow space 103. The sealed high-pressure gas can effectively prevent the secondary piston 106 from moving downward, thereby preventing insufficient gas pressure caused by the downward movement of the main piston 92, and preventing the embryo from dripping and coming into contact with the outside. When it is necessary to discharge the embryo, similarly, downward pressure is applied to the push plate 1110, so that there is a certain gap between the secondary funnel-shaped boss structure 119 and the conical surface of the secondary funnel-shaped cavity 114. At this time,High-pressure gas located inside the annular gas flow space 103 is discharged to the outside space through this gap. The main piston 92, under the action of the main helical spring 108, can move downwards to discharge the embryo. During the discharge process, by controlling the size of the gap between the secondary funnel-shaped boss structure 119 and the conical surface of the secondary funnel-shaped cavity 114, the speed at which the high-pressure gas is discharged outwards can be controlled, thereby controlling the embryo discharge speed.

[0036] In use, the bottom opening of the embryo suction port 84 is immersed into the embryo, and the pull plate 98 is pulled upwards. This increases the space below the main piston 92, causing a decrease in air pressure above the sheet-like elastic gas membrane 810. Simultaneously, the lowering of the space above the main piston 92 allows air to flow through the gas flow channel 6 and the first gas flow port 88 into the annular gas compression space 86, thereby increasing the gas pressure in the outer region of the annular elastic gas membrane 89. During this process, when the air pressure above the sheet-like elastic gas membrane 810 drops to a negative pressure value below atmospheric pressure, the external atmospheric pressure will cause the sheet-like elastic gas membrane 810 to deform upwards, thus drawing the embryo into the concave area of ​​the sheet-like elastic gas membrane 810, achieving embryo suction. Meanwhile, when the periphery of the annular elastic gas membrane 89 is affected by high-pressure gas, the annular elastic gas membrane 89 will change towards its center until the annular hole of the annular elastic gas membrane 89 closes, thus achieving the blocking effect on the embryo. Then, holding the device, the embryo suction hole 84 is aligned with the area to be discharged, and downward pressure is applied to the push plate 1110, so that there is a certain gap between the secondary funnel-shaped protrusion structure 119 and the conical surface of the secondary funnel-shaped cavity 114. At this time, the high-pressure gas inside the annular gas flow space 103 will be discharged to the outside space along the gap, and the main piston 92 can move down under the action of the main helical spring 108. At the same time, the sheet-like elastic gas membrane 810 can reset according to its own elasticity, thereby enabling the embryo to be discharged.

[0037] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. An in vitro embryo detection device, comprising a cylindrical hollow shell (1), a hollow rod structure (2) disposed at the top of the cylindrical hollow shell (1), a component embedding groove (3) disposed at the bottom of the cylindrical hollow shell (1), a main funnel-shaped cavity (4) disposed at the top of the component embedding groove (3), a main longitudinal reciprocating cavity (5) disposed at the top of the main funnel-shaped cavity (4), a gas flow channel (6) disposed inside the cylindrical hollow shell (1) and communicating with the top of the main longitudinal reciprocating cavity (5) and the bottom of the cylindrical hollow shell (1), and a shaft through hole (7) disposed inside the hollow rod structure (2), characterized in that: Also includes The air-film embryo retrieval structure (8) has a sheet-like elastic air film (810) that deforms upward under the negative pressure of the gas above and sucks the embryo into the deformed depression area, and an annular elastic air film (89) that deforms towards the center after being subjected to gas pressure on the periphery and blocks and closes the central area. And a piston-type gas guiding structure (9) is placed inside the main longitudinal reciprocating cavity (5). Inside the cavity is a main piston (92) that can move axially along the main longitudinal reciprocating cavity (5) and, during the movement, reduces the gas pressure in the space above the sheet-like elastic gas film (810) and increases the gas pressure in the outer region of the annular elastic gas film (89). It also includes a gas-confined reset structure (10), which includes an annular hollow shell (101). The center of the annular hollow shell (101) is provided with a central sleeve hole (102) that can be fixedly fitted around the hollow rod structure (2). An annular gas flow space (103) is provided in the bottom area of ​​the annular solid region of the annular hollow shell (101). A component mounting hole (109) is provided in the solid region above the annular gas flow space (103) inside the annular hollow shell (101). The bottom end of the component mounting hole (109) is connected to the top end of the annular gas flow space (103), and the top end is open. It also includes an elastic gas flow control structure (11), which includes a cylindrical hollow body (111) that can be fixedly placed inside the component mounting hole (109). The cylindrical hollow body (111) and the component mounting hole (109) are filled with sealant at the mounting gap. The cylindrical hollow body (111) has a longitudinal hollow cavity (112) inside. The cylindrical hollow body (111) has a third gas flow hole (113) at the bottom end of the longitudinal hollow cavity (112). The cylindrical hollow body (111) has an inverted secondary funnel-shaped cavity (114) at the bottom end of the third gas flow hole (113). The bottom end of the secondary funnel-shaped cavity (114) is connected to the top end of the annular gas flow space (103).

2. The in vitro embryo retrieval device according to claim 1, characterized in that: The air-film embryo retrieval structure (8) includes an embryo aspiration needle (81). The top of the embryo aspiration needle (81) is provided with a cylindrical protrusion (82) that can be inserted into a component embedding groove (3). A sealing ring (83) is provided on the circumferential side of the cylindrical protrusion (82). An embryo aspiration hole (84) communicating with its bottom is provided at the center of the embryo aspiration needle (81). A rod insertion groove (85) with an opening at the top and communicating with the embryo aspiration hole (84) at the bottom is provided inside the cylindrical protrusion (82). An annular gas compression space (86) is provided around the bottom opening of the embryo aspiration hole (84) inside the embryo aspiration needle (81). The upper end face is provided with a plurality of integral structures (87) that can be inserted into the bottom of the gas flow channel (6) on the periphery of the cylindrical protrusion structure (82). The insertion rod structure (87) and the embryo aspiration needle (81) are provided with a first gas flow hole (88) that connects the space above the insertion rod structure (87) and the annular gas compression space (86). The embryo aspiration needle (81) is embedded with an annular elastic gas membrane (89) at the annular intersection of the embryo aspiration hole (84) and the annular gas compression space (86). The embryo aspiration needle (81) is embedded with a sheet-like elastic gas membrane (810) above the annular elastic gas membrane (89) inside the embryo aspiration hole (84).

3. The in vitro embryo retrieval device according to claim 2, characterized in that: The sealing ring (83) can be inserted into the component embedding groove (3), and after insertion, the sealing ring (83) can seal the installation gap.

4. The in vitro embryo retrieval device according to claim 2, characterized in that: Both the annular elastic air membrane (89) and the sheet-like elastic air membrane (810) are made of elastic materials that are prone to deformation.

5. The in vitro embryo retrieval device according to claim 2, characterized in that: The piston-type gas guiding structure (9) includes a movable shaft (91) that is inserted into the shaft through hole (7) and can move axially along the shaft through hole (7). A main piston (92) is installed at one end of the movable shaft (91) located inside the main longitudinal reciprocating cavity (5). A main funnel-shaped boss structure (94) matching the main funnel-shaped cavity (4) is provided at the bottom end of the main funnel-shaped boss structure (94). A columnar valve stem (95) with an integral structure that can be inserted into the rod insertion slot (85) is provided at the bottom end of the main funnel-shaped boss structure (94). An array-shaped wing-shaped mounting plate (93) is installed at the end of the movable shaft (91) located above the hollow rod structure (2). The area between every two wing-shaped structures in the array-shaped wing-shaped mounting plate (93) forms a fan-shaped notch (96). A pull rod (97) is installed at the center of the top of the array-shaped wing-shaped mounting plate (93). A pull plate (98) is installed at the top of the pull rod (97).

6. The in vitro embryo retrieval device according to claim 5, characterized in that: A sealing ring is fitted on the circumferential side of the main piston (92), and a sealing ring is embedded in the shaft through hole (7) on the periphery of the moving shaft (91).

7. The in vitro embryo retrieval device according to claim 5, characterized in that: The interior of the annular hollow shell (101) has three annular array-type secondary longitudinal reciprocating cavities (105) in a solid region above the annular gas flow space (103). The interior of the annular hollow shell (101) has a second gas flow hole (104) connecting the bottom of the secondary longitudinal reciprocating cavity (105) and the top of the annular gas flow space (103). Each secondary longitudinal reciprocating cavity (105) has a secondary piston (106) that can move axially along the secondary longitudinal reciprocating cavity (105). Each secondary piston (106) has a telescopic rod (107) that penetrates the top structure of the annular hollow shell (101) installed on its upper end face. The top of each telescopic rod (107) is fixedly connected to the bottom surface of a wing structure of the array-shaped wing mounting plate (93) through a connecting plate. Each telescopic rod (107) has a main helical spring (108) in a compressed state on the rod body located inside the secondary longitudinal reciprocating cavity (105).

8. The in vitro embryo retrieval device according to claim 7, characterized in that: A sealing ring is fitted onto the circumferential side of the auxiliary piston (106).

9. The in vitro embryo retrieval device according to claim 7, characterized in that: The top of the cylindrical hollow body (111) is provided with a plurality of fourth gas flow holes (115) connecting the longitudinal hollow cavity (112) and the space above the cylindrical hollow body (111). Inside the secondary funnel-shaped cavity (114) is a secondary funnel-shaped boss structure (119) that can move longitudinally and matches the structural shape of the secondary funnel-shaped cavity (114). At the top of the secondary funnel-shaped boss structure (119) is installed a longitudinal rod (1111) located inside the third gas flow hole (113) and the longitudinal hollow cavity (112). The radius of the longitudinal rod (1111) is smaller than the structural radius of the third gas flow hole (113). (1111) A movable plate (116) is installed at the top of the longitudinal hollow cavity (112). The structural radius of the longitudinal hollow cavity (112) is larger than the structural radius of the movable plate (116). A secondary helical spring (118) in a compressed state is placed around the rod body (1111) inside the longitudinal hollow cavity (112). There is a moving gap between the upper end face of the movable plate (116) and the top of the longitudinal hollow cavity (112). A push rod (117) penetrating the central structure of the top of the cylindrical hollow body (111) is installed on the upper end face of the movable plate (116). A push plate (1110) is installed at the top of the push rod (117).

10. The in vitro embryo retrieval device according to claim 9, characterized in that: In the initial state, the elastic strength of the secondary helical spring (118) is greater than the sum of the elastic strengths of the three main helical springs (108).

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