A pmt detection device and method based on led lamp

Abstract

The application relates to the technical field of pmt detection, and discloses a pmt detection device based on an LED lamp and a detection method, wherein the pmt detection device based on the LED lamp comprises a detection device body, a mounting cavity arranged on one side of an inner cavity of the detection device body, an operation bin door arranged on one end of one side of the detection device body, and a sample table arranged on the bottom of an inner cavity of the mounting cavity. The pmt detection device based on the LED lamp is provided with multiple groups of LED lamp tubes with different wavelengths, can switch the LED lamp tubes with different wavelengths for detection according to actual requirements, can match the excitation requirements of specific fluorescent markers by adjusting the wavelength of excitation light, can increase the intensity of fluorescent signals and the sensitivity of detection, and can study and observe the reactions and behaviors of photosensitive microorganisms under different wavelengths by adjusting the wavelength of excitation light, so as to deeply understand the biological characteristics of the photosensitive microorganisms.

Description

Technical Field

[0001] This invention relates to the field of PMT detection technology, and in particular to a PMT detection device and method based on LED lights. Background Technology

[0002] PMT detection refers to a technology that uses photomultiplier tubes to detect and enhance photoelectric signals. A PMT is a highly sensitive photodetector, typically composed of a photocathode, a series of multiplying electrodes, and a collecting electrode. When a photon shines on the photocathode, the photon is converted into an electron. The photoelectron then passes through the multiplying electrode system, where it is amplified step by step through an electron multiplication process. In a PMT, the photoelectron undergoes a cascade amplification process through the multiplying electrodes, and the original photon signal can be amplified thousands of times or even more. Due to this high-gain characteristic, a PMT can detect very weak light signals and convert them into electrical signals for output.

[0003] Therefore, PMT detection is widely used in many fields, such as physics, astronomy, biology, and medicine. In the medical field, PMT detection has multiple applications, such as biofluorescence imaging, flow cytometry, molecular diagnostics, and nuclear medicine. PMT (photomultiplier tube) detection technology can also be used for the detection of medical microbial infections. Microbial infection is a disease caused by microorganisms such as bacteria, viruses, fungi, or parasites. PMT detection technology can be used to detect and monitor biomarkers, antigens, antibodies, or other related components associated with microbial infection, thereby enabling rapid diagnosis and monitoring of infection. In the detection of microbial infection, PMT can be used to detect fluorescent markers or chemiluminescent signals produced by microorganisms. These markers or signals can be associated with the presence or activity of microorganisms through specific optical reactions, and then converted into electrical signals by the PMT detector for quantitative analysis.

[0004] Currently, most PMT detection devices use LEDs (light-emitting diodes) as the light source for detecting microbial infections. In microbial infection detection, fluorescent labels are often used to detect microorganisms or their related components. Fluorescent labels need to be excited by a suitable light source with a specific wavelength of light, which requires a suitable LED to provide the corresponding excitation light. However, most LEDs in current PMT detection devices are in a group, and their wavelengths are mostly the same. When the wavelength of the LED does not match the requirements of the label during detection, it may not be able to effectively excite fluorescence, resulting in a weak detection signal or inaccurate results. Summary of the Invention

[0005] Given that the LEDs in most existing PMT detection devices are in groups, when the wavelength of the LEDs does not match the requirements of the marker, it can easily lead to a weak detection signal or an inability to obtain accurate results. Therefore, this invention is proposed.

[0006] Therefore, the purpose of this invention is to provide a PMT detection device based on LED lights, which aims to: match LED lights of different wavelengths according to the requirements of the marker, improve adaptability, increase detection signal and improve the accuracy of detection results.

[0007] To solve the above-mentioned technical problems, the present invention provides the following technical solution: a PMT detection device based on LED lights, which includes a detection device body and an installation cavity opened on one side of the inner cavity of the detection device body, an operation door disposed at one end of one side of the detection device body, a stage disposed at the bottom of the inner cavity of the installation cavity, a photodetector disposed at one end of the inner cavity of the installation cavity, a wavelength conversion component disposed in the inner cavity of the installation cavity for adjusting the wavelength, and a heat dissipation component disposed on the wavelength conversion component for heat dissipation; The wavelength conversion component includes an annular groove disposed at the top of one end of both sides of the mounting cavity, multiple sets of sliders disposed inside the annular groove, a mounting plate disposed on one side of the sliders, five sets of LED tubes disposed on the side of the two sets of mounting plates close to each other, and a connecting pipe disposed on the side of the two sets of mounting plates close to each other.

[0008] As a preferred embodiment of the PMT detection device based on LED lights according to the present invention, wherein: both ends of the connecting tube penetrate the mounting plate, and one end of the connecting tube extends to the outside of the detection device body, and a limit component is provided at the end of the connecting tube located outside the detection device body.

[0009] As a preferred embodiment of the PMT detection device based on LED lights according to the present invention, the limiting component includes a fixed plate disposed on the surface of the connecting tube located on the outer end of the detection device body, a sliding plate disposed on the surface of the connecting tube located on the outer end of the detection device body, and a circular handle disposed on the surface of the sliding plate.

[0010] As a preferred embodiment of the PMT detection device based on LED lights according to the present invention, the limiting component further includes five sets of fixing rods disposed on the edge of the sliding disk near the detection device body, and five sets of fixing slots disposed on one side of the surface of the detection device body, wherein the fixing rods and fixing slots are mutually compatible, and the connecting tube is located between the five sets of fixing slots.

[0011] As a preferred embodiment of the PMT detection device based on LED lights described in this invention, the limiting component further includes multiple sets of telescopic rods disposed on the edge of the fixed plate near the sliding plate, with one end of the telescopic rod connected to one side of the sliding plate; a spring disposed on the surface of the telescopic rod; multiple sets of hexagonal slots disposed on the surface of the fixed plate, with the hexagonal slots penetrating the fixed plate; and multiple sets of hexagonal posts disposed on the side of the sliding plate near the fixed plate, with the hexagonal posts and hexagonal slots mutually compatible.

[0012] As a preferred embodiment of the PMT detection device based on LED lamps according to the present invention, the heat dissipation component includes a heat dissipation guide plate disposed on the surface of the LED lamp tube, multiple sets of guide plates disposed on the heat dissipation guide plate near one end of the connecting pipe, and the guide plates slide to the inner cavity of the connecting pipe, two sets of heat dissipation holes disposed on one side of the surface of the guide plates, and the heat dissipation holes penetrate the guide plates, and a heat conduction component disposed on the connecting pipe.

[0013] As a preferred embodiment of the PMT detection device based on LED lights according to the present invention, the heat conduction component includes pentagonal sleeves disposed at both ends of the surface of the connecting pipe, five sets of positioning rods disposed on the surface of the pentagonal sleeves, one end of the positioning rods being connected to the heat dissipation guide plate, and the other end of the positioning rods extending into the inner cavity of the connecting pipe, a second spring disposed at one end of the positioning rods located in the inner cavity of the connecting pipe, and a blocking block disposed on the surface of the positioning rods located in the inner cavity of the connecting pipe.

[0014] As a preferred embodiment of the PMT detection device based on LED lights according to the present invention, wherein: a fan is provided at one end of the inner cavity of the connecting pipe located inside the mounting cavity, a fan is provided at the other end of the inner cavity of the connecting pipe located outside the detection device body, and a grid is provided at the other end of the connecting pipe located outside the detection device body.

[0015] The present invention also provides a detection method for a PMT detection device based on LED lights, the purpose of which is to provide good heat dissipation management, avoid excessive heat generation of LED lights, thereby improving their efficiency and output light intensity, ensure good heat dissipation around LED lights, avoid excessive heat generation, and improve the stability of LEDs during use.

[0016] To achieve the above objectives, the present invention provides the following technical solution: a detection method for a PMT detection device based on LED lights, comprising the following steps: First, open the operating chamber door, then place the microorganisms on the stage and close the operating chamber door. Then, detect the microorganisms by using a photoelectric detector to detect the fluorescent markers or chemiluminescent signals produced by the microorganisms, and use the wavelength of the LED tube to excite the fluorescent markers of the microorganisms. When testing different microorganisms, if the wavelength of the current LED tube does not match the requirements of the marker, pulling the circular handle compresses the telescopic rod and spring, fixing the sliding plate and the fixed plate relative to each other and releasing the limit on multiple sets of LED tubes. Then, the circular handle can be rotated to rotate the five sets of LED tubes. After rotating the corresponding LED tube to the position, the circular handle can be released to fix the LED tube, and then the testing operation can continue. When using LED tubes as a light source for illumination and testing for an extended period of time, by activating fan one and fan two, fan one can blow out air while fan two draws out hot air from the connecting pipe. The heat dissipation plate and guide plate can be used to quickly expel the heat from the LED tubes from the connecting pipe into the testing device body, thereby reducing the operating temperature of the LED tubes. At the same time, by utilizing the elasticity of the blocking block, the heat dissipation guide plate on the positioning rod can be pushed to fit tightly against the surface of the LED tube, allowing the heat dissipation guide plate to more comprehensively absorb and disperse the heat generated by the LED tube. Once the test is complete, open the operating chamber door and remove the microorganisms from the stage.

[0017] The beneficial effects of this invention are: 1. By setting up multiple sets of LED tubes with different wavelengths, different wavelengths of LED tubes can be switched for detection according to actual needs. By adjusting the wavelength of the excitation light, the excitation requirements of specific fluorescent markers can be matched, thereby increasing the intensity of the fluorescence signal and the sensitivity of detection. Some photosensitive microorganisms have different reactions or physiological effects to different wavelengths of light. By adjusting the wavelength of the excitation light, the reactions and behaviors of photosensitive microorganisms at different wavelengths can be studied and observed, thereby gaining a deeper understanding of their biological characteristics.

[0018] 2. By utilizing heat dissipation plates and guide plates, heat from the LED tube is quickly dissipated from the connecting pipe into the detection device body. This effectively reduces the operating temperature of the LED tube, minimizing heat damage and degradation, thus extending its lifespan. Proper heat dissipation also keeps the LED tube temperature at a low level, reducing the impact of temperature fluctuations. Furthermore, light quality refers to the spectrum and wavelength characteristics of the light emitted by the LED tube. Appropriate heat dissipation reduces the impact of light source temperature on light quality, maintaining the stability and consistency of the LED tube's output light, thereby improving the stability of the LED tube's operation. A stable light source is crucial for detecting microbial infections, ensuring accurate and repeatable measurement results.

[0019] 3. By utilizing the elasticity of the blocking block, the heat dissipation guide plate on the positioning rod can be pushed to fit tightly against the surface of the LED tube. This allows the heat dissipation guide plate to more effectively absorb and disperse the heat from the LED tube, thereby promoting heat dissipation. Furthermore, through close contact, the heat dissipation guide plate can more comprehensively absorb and disperse the heat generated by the LED tube, avoiding problems such as excessively high local hot spots and heat accumulation, maintaining a uniform overall heat dissipation effect, and thus improving the heat dissipation efficiency of the heat dissipation guide plate. Attached Figure Description

[0020] Figure 1 This is a schematic diagram of the overall structure of the PMT detection device based on LED lights according to the present invention.

[0021] Figure 2 This is a side-view cross-sectional three-dimensional structural diagram of the PMT detection device based on LED lights according to the present invention.

[0022] Figure 3 This is a rear cross-sectional three-dimensional structural diagram of the PMT detection device based on LED lights according to the present invention.

[0023] Figure 4 For the present invention Figure 1 A magnified structural diagram at point A.

[0024] Figure 5 This is a three-dimensional structural diagram of the wavelength conversion component of the LED-based PMT detection device of the present invention.

[0025] Figure 6 This is a cross-sectional three-dimensional structural diagram of the heat dissipation component of the PMT detection device based on LED lights according to the present invention.

[0026] Figure 7 This is a first cross-sectional perspective view of the connecting tube of the PMT detection device based on LED lights according to the present invention.

[0027] Figure 8 This is a second cross-sectional perspective view of the three-dimensional structure of the connecting tube of the PMT detection device based on LED lights according to the present invention.

[0028] Explanation of reference numerals in the attached figures: 1. Detection device body; 2. Mounting cavity; 3. Photodetector; 4. Stage; 5. Operating chamber door; 6. Wavelength conversion component; 61. Annular groove; 62. Slider; 63. Mounting plate; 64. LED tube; 65. Connecting pipe; 66. Limiting component; 661. Fixed plate; 662. Sliding plate; 663. Fixed insertion rod; 664. Fixed slot; 665. Telescopic rod; 666. Spring 1; 667. Hexagonal slot; 668. Hexagonal column; 669. Circular throttle; 7. Heat dissipation component; 71. Heat dissipation guide plate; 72. Guide plate; 73. Fan 1; 74. Heat dissipation hole; 75. Fan 2; 76. Grille; 77. Heat conduction component; 771. Pentagonal sleeve; 772. Positioning rod; 773. Blocking block; 774. Spring 2. Detailed Implementation

[0029] Example 1 Reference Figure 1-4 The first embodiment of the present invention provides a PMT detection device based on an LED light. This PMT detection device based on an LED light includes a detection device body 1 and an installation cavity 2 opened on one side of the inner cavity of the detection device body 1, an operation door 5 hinged to one end of one side of the detection device body 1, a handle provided on one end of one side of the operation door 5, a platform 4 set at the bottom of the inner cavity of the installation cavity 2 for placing microorganisms, a photodetector 3 set at one end of the inner cavity of the installation cavity 2, a wavelength conversion component 6 set in the inner cavity of the installation cavity 2 for adjusting the wavelength, and a heat dissipation component 7 set on the wavelength conversion component 6 for heat dissipation. The wavelength conversion component 6 includes an annular groove 61 formed at the top of one end of both sides of the inner cavity of the mounting cavity 2, multiple sets of sliders 62 slidably connected to the inner side of the annular groove 61, a mounting plate 63 fixedly installed on one side of the slider 62, five sets of LED tubes 64 arranged on the side of the two sets of mounting plates 63 close to each other, the five sets of LED tubes 64 having different wavelengths, and a connecting pipe 65 fixedly installed on the side of the two sets of mounting plates 63 close to each other. Both ends of the connecting pipe 65 pass through the mounting plate 63, and one end of the connecting pipe 65 extends to the outside of the detection device body 1. A limit component 66 is provided at the end of the connecting pipe 65 located outside the detection device body 1.

[0030] The limiting component 66 includes a fixed plate 661 fixedly sleeved on the outer surface of the connecting tube 65 at one end of the detection device body 1, a sliding plate 662 slidably sleeved on the outer surface of the connecting tube 65 at one end of the detection device body 1, and a circular handle 669 mounted on the surface of the sliding plate 662. The limiting component 66 also includes five sets of fixed inserts 663 mounted on the edge of the sliding plate 662 near the detection device body 1, and five sets of fixed slots 664 opened on one side of the surface of the detection device body 1. The fixed inserts 663 and the fixed slots 664 are mutually compatible. The connecting tube 65 is located between the five sets of fixed slots 664. Five sets of pointers are provided on the surface of the sliding plate 662. A positioning block is provided on one side of the detection device body 1 near the connecting tube 65 to determine that the current LED tube 64 is in the detection and use state.

[0031] The limiting component 66 also includes multiple sets of telescopic rods 665 installed on the edge of the fixed plate 661 near the sliding plate 662, with one end of the telescopic rod 665 connected to one side of the sliding plate 662, a spring 666 sleeved on the surface of the telescopic rod 665, multiple sets of hexagonal slots 667 opened on the surface of the fixed plate 661, with the hexagonal slots 667 penetrating the fixed plate 661, and multiple sets of hexagonal posts 668 provided on the side of the sliding plate 662 near the fixed plate 661, with the hexagonal posts 668 and the hexagonal slots 667 mutually compatible.

[0032] During use, the operating chamber door 5 is first opened, then the microorganisms are placed on the stage 4 and the operating chamber door 5 is closed. When detecting the microorganisms, the fluorescent markers or chemiluminescent signals produced by the microorganisms can be detected by the photodetector 3, and then converted into electrical signals by the PMT detector for quantitative analysis. At the same time, a set of LED tubes 64 are turned on, and the wavelength of the LED tubes 64 is used to excite the fluorescent markers of the microorganisms. This makes the PMT detection technology a useful tool for the detection and research of microbial infections, providing high sensitivity and quantitative analysis capabilities, which helps in the early diagnosis and treatment of microbial infections. When detecting different microorganisms, if the wavelength of the current LED tube 64 does not match the requirements of the marker, the circular handle 669 can be gripped and pulled to compress the telescopic rod 665 and spring 666. Simultaneously, the hexagonal post 668 will penetrate the hexagonal slot 667, thus limiting and fixing the sliding plate 662 and the fixed plate 661. This also causes the five sets of fixing rods 663 on the sliding plate 662 to move out of the five sets of fixing slots 664 on one side of the detection device body 1, releasing the limitation on multiple sets of LED tubes 64. Rotating the circular handle 669 then rotates the LED tubes 64, which in turn rotates the two mounting plates 63, causing the slider 62 to rotate within the annular groove 61. Finally, all five sets of LED tubes 64 can be rotated, allowing the appropriate wavelength to be selected. The LED tube 64 is moved to the corresponding position on the positioning block for easy subsequent testing. Then, simply release the circular handle 669, and the spring force 666 will drive the sliding plate 662 to reset, pushing the fixing rod 663 back into the fixing slot 664, thus fixing the LED tube 64. Testing can then continue. Since different wavelengths of LED tubes 64 can be switched for testing according to actual needs, the excitation wavelength can be adjusted to match the excitation requirements of specific fluorescent markers, thereby increasing the intensity of the fluorescence signal and the sensitivity of detection. Some photosensitive microorganisms have different reactions or physiological effects to different wavelengths of light. By adjusting the excitation wavelength, the reactions and behaviors of photosensitive microorganisms at different wavelengths can be studied and observed, thereby gaining a deeper understanding of their biological characteristics.

[0033] Example 2 Reference Figure 1-7 This is the second embodiment of the present invention. This embodiment differs from the first embodiment in that: the heat dissipation component 7 includes a heat dissipation guide plate 71 disposed on the surface of the LED tube 64; multiple sets of guide plates 72 disposed on the heat dissipation guide plate 71 near one end of the connecting pipe 65, with the guide plates 72 slidingly extending into the inner cavity of the connecting pipe 65; two sets of heat dissipation holes 74 opened on one side of the surface of the guide plates 72 to increase the contact area between the guide plates 72 and the air for heat dissipation; the heat dissipation holes 74 penetrate the guide plates 72; and a heat-conducting component 77 disposed on the connecting pipe 65. A fan 73 is disposed at one end of the inner cavity of the connecting pipe 65 located within the mounting cavity 2, and a fan 75 is disposed at the other end of the inner cavity of the connecting pipe 65 located outside the detection device body 1. A grille 76 is disposed at the other end of the connecting pipe 65 located outside the detection device body 1 to prevent operators from accidentally inserting their hands into the connecting pipe 65 and being cut by the fan 75.

[0034] During use, when the LED tube 64 is continuously illuminated for testing, heat is generated on its surface. Firstly, through contact with the heat dissipation plate 71, the heat is absorbed from the LED tube 64 onto the heat dissipation plate 71. Then, the heat is transferred from the heat dissipation plate 71 to the connecting pipe 65 via the guide plate 72. At this point, by activating fans 1 73 and 2 75, fan 1 blows air while fan 2 draws hot air from the connecting pipe 65, thus quickly extracting and expelling the heat transferred from the LED tube 64 into the connecting pipe 65, effectively reducing the heat generated by the LED tube 64. The operating temperature of the LED tube 64 is controlled to reduce heat damage and degradation, thereby extending its lifespan. Proper heat dissipation keeps the LED tube 64's temperature low, minimizing the impact of temperature fluctuations. Furthermore, light quality refers to the spectrum and wavelength characteristics of the light emitted by the LED tube 64. Appropriate heat dissipation reduces the influence of light source temperature on light quality, maintaining the stability and consistency of the LED tube 64's output light, thus improving its operational stability. A stable light source is crucial for detecting microbial infections, ensuring accurate and repeatable measurement results.

[0035] The remaining structure is the same as that in Example 1.

[0036] Example 3 Reference Figure 1-8 This is the third embodiment of the present invention. The difference between this embodiment and the second embodiment is that the heat-conducting component 77 includes a pentagonal sleeve 771 fixedly sleeved on both ends of the surface of the connecting pipe 65, five sets of positioning rods 772 slidably connected to the surface of the pentagonal sleeve 771, one end of the positioning rod 772 being connected to the heat dissipation guide plate 71, and the other end of the positioning rod 772 extending into the inner cavity of the connecting pipe 65, a spring 774 sleeved on one end of the positioning rod 772 located in the inner cavity of the connecting pipe 65, and a blocking block 773 fixedly installed on the surface of the positioning rod 772 located in the inner cavity of the connecting pipe 65.

[0037] During use, by utilizing the elasticity of the blocking block 773, the heat dissipation guide plate 71 on the positioning rod 772 can be pushed to fit tightly against the surface of the LED tube 64, so that the heat dissipation guide plate 71 can more effectively absorb and disperse the heat from the LED tube 64, thereby promoting the heat dissipation effect. Moreover, through close contact, the heat dissipation guide plate 71 can more comprehensively absorb and disperse the heat generated by the LED tube 64, avoiding the problem of excessively high local hot spots and heat accumulation, maintaining a uniform overall heat dissipation effect, thereby improving the heat dissipation efficiency of the heat dissipation guide plate 71.

[0038] The remaining structure is the same as that in Example 2.

[0039] Example 4 Reference Figure 1-8 The fourth embodiment of the present invention provides a detection method for a PMT detection device based on LED lights, comprising the following steps: S1. First, open the operating chamber door 5, then place the microorganisms on the stage 4 and close the operating chamber door 5. Then, detect the microorganisms by using the photodetector 3 to detect the fluorescent markers or chemiluminescent signals produced by the microorganisms, and use the wavelength of the LED tube 64 to excite the fluorescent markers of the microorganisms. S2, When detecting different microorganisms, if the wavelength of the current LED tube 64 does not match the requirements of the marker, the telescopic rod 665 and spring 666 are compressed by pulling the circular handle 669, so that the sliding plate 662 and the fixed plate 661 are relatively limited and fixed, and the limitation on multiple sets of LED tubes 64 is canceled. Then, the circular handle 669 can be rotated to drive the five sets of LED tubes 64 to rotate. After the corresponding LED tube 64 is rotated to the appropriate position, the circular handle 669 is released to fix the LED tube 64, and then the detection operation continues. S3, when the LED tube 64 is used as a light source for illumination and detection for a long time, by starting the fan 1 73 and the fan 2 75, the fan 1 73 can blow out air, and the fan 2 75 can draw out the hot air in the connecting pipe 65. The heat dissipation plate 71 and the guide plate 72 can be used to quickly discharge the heat on the LED tube 64 from the connecting pipe 65 into the detection device body 1, thereby reducing the working temperature of the LED tube 64. S4. At the same time, by utilizing the elasticity of the blocking block 773, the heat dissipation guide plate 71 on the positioning rod 772 can be pushed to fit tightly against the surface of the LED tube 64, allowing the heat dissipation guide plate 71 to absorb and disperse the heat generated by the LED tube 64 more comprehensively. S5. After the test is completed, open the operation chamber door 5 and then the microorganisms on the stage 4 can be taken out.

Claims

1. A PMT detection device based on LED lights, comprising a detection device body (1) and a mounting cavity (2) formed on one side of the inner cavity of the detection device body (1), and an operating door (5) hinged to one end of the detection device body (1), characterized in that: A stage (4) is provided at the bottom of the inner cavity of the mounting cavity (2), a photodetector (3) is provided at one end of the top of the inner cavity of the mounting cavity (2), a wavelength conversion component (6) is provided in the inner cavity of the mounting cavity (2) for adjusting the wavelength, and a heat dissipation component (7) is provided on the wavelength conversion component (6) for heat dissipation. The wavelength conversion component (6) includes an annular groove (61) disposed at the top of one end of the inner cavity of the mounting cavity (2), multiple sets of sliders (62) disposed inside the annular groove (61), a mounting plate (63) disposed on one side of the slider (62), five sets of LED tubes (64) disposed on the side of the two sets of mounting plates (63) close to each other, and a connecting pipe (65) disposed on the side of the two sets of mounting plates (63) close to each other. Both ends of the connecting pipe (65) pass through the mounting plate (63), and one end of the connecting pipe (65) extends to the outside of the detection device body (1). A limit component (66) is provided at the end of the connecting pipe (65) located outside the detection device body (1). The limiting component (66) includes a fixed plate (661) disposed on the outer end surface of the connecting tube (65) located on the detection device body (1), a sliding plate (662) disposed on the outer end surface of the connecting tube (65) located on the detection device body (1), and a circular throttle (669) disposed on the surface of the sliding plate (662). The limiting component (66) also includes five sets of fixing rods (663) disposed on the edge of the sliding disk (662) near the detection device body (1), and five sets of fixing slots (664) disposed on one side of the surface of the detection device body (1), and the fixing rods (663) and fixing slots (664) are mutually compatible, and the connecting pipe (65) is located between the five sets of fixing slots (664); The heat dissipation component (7) includes a heat dissipation guide plate (71) disposed on the surface of the LED tube (64), multiple sets of guide plates (72) disposed on the heat dissipation guide plate (71) near the end of the connecting tube (65), and the guide plates (72) slide to the inner cavity of the connecting tube (65), two sets of heat dissipation holes (74) disposed on one side of the surface of the guide plates (72), and the heat dissipation holes (74) penetrate the guide plates (72), and a heat conduction component (77) disposed on the connecting tube (65).

2. The PMT detection device based on LED lights according to claim 1, characterized in that: The limiting component (66) further includes multiple sets of telescopic rods (665) disposed on the edge of the fixed plate (661) near the sliding plate (662), with one end of the telescopic rod (665) connected to one side of the sliding plate (662), a spring (666) disposed on the surface of the telescopic rod (665), multiple sets of hexagonal slots (667) disposed on the surface of the fixed plate (661), with the hexagonal slots (667) penetrating the fixed plate (661), and multiple sets of hexagonal posts (668) disposed on the side of the sliding plate (662) near the fixed plate (661), with the hexagonal posts (668) and the hexagonal slots (667) mutually adapted to each other.

3. The PMT detection device based on LED lights according to claim 2, characterized in that: The heat-conducting component (77) includes a pentagonal sleeve (771) disposed at both ends of the surface of the connecting pipe (65), five sets of positioning rods (772) disposed on the surface of the pentagonal sleeve (771), one end of the positioning rod (772) being connected to the heat dissipation guide plate (71), and the other end of the positioning rod (772) extending into the inner cavity of the connecting pipe (65), a spring (774) disposed at one end of the positioning rod (772) located in the inner cavity of the connecting pipe (65), and a blocking block (773) disposed on the surface of the positioning rod (772) located in the inner cavity of the connecting pipe (65).

4. The PMT detection device based on LED lights according to claim 3, characterized in that: A fan (73) is provided at one end of the inner cavity of the connecting pipe (65) located inside the mounting cavity (2), a fan (75) is provided at one end of the inner cavity of the connecting pipe (65) located outside the detection device body (1), and a grid (76) is provided at one end of the connecting pipe (65) located outside the detection device body (1).

5. A detection method for a PMT detection device based on LED lights, employing the PMT detection device based on LED lights as described in claim 4, characterized in that: Includes the following steps: First, open the operating chamber door (5), then place the microorganisms on the stage (4) and close the operating chamber door (5). Then, detect the microorganisms by using a photodetector (3) to detect the fluorescent markers or chemiluminescent signals produced by the microorganisms, and use the wavelength of the LED tube (64) to excite the fluorescent markers of the microorganisms. When different microorganisms are being tested, if the wavelength of the current LED tube (64) does not match the requirements of the marker, the telescopic rod (665) and spring 1 (666) are compressed by pulling the circular handle (669), so that the sliding plate (662) and the fixed plate (661) are relatively limited, and the limitation on multiple sets of LED tubes (64) is canceled. Then, the circular handle (669) is rotated to drive the five sets of LED tubes (64) to rotate. When the corresponding LED tube (64) is rotated to the position, the circular handle (669) is released to fix the LED tube (64), and then the testing operation continues.

6. The detection method of the PMT detection device based on LED lights according to claim 5, characterized in that: When the LED tube (64) is used as a light source for a long time for illumination and detection, by starting the first fan (73) and the second fan (75), the first fan (73) blows out the air, while the second fan (75) draws out the hot air in the connecting pipe (65). The heat dissipation plate (71) and the guide plate (72) are used to quickly discharge the heat on the LED tube (64) from the connecting pipe (65) into the detection device body (1), and then reduce the working temperature of the LED tube (64). At the same time, by utilizing the elasticity of the blocking block (773), the heat dissipation guide plate (71) on the positioning rod (772) is pushed to stick tightly to the surface of the LED tube (64), so that the heat dissipation guide plate (71) can more comprehensively absorb and disperse the heat generated by the LED tube (64); Once the test is complete, open the operating chamber door (5) and then remove the microorganisms from the stage (4).

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