Medical clinical microorganism PCR detection device

Abstract

The application discloses a medical clinical microorganism PCR detection device and relates to the technical field of microorganim PCR detection.The medical clinical microorganism PCR detection device comprises a constant-temperature culture shaking table, the constant-temperature culture shaking table comprises a constant-temperature culture shaking table device body and a control system, the constant-temperature culture shaking table device body comprises a first box body, an orbit assembly is arranged in the first box body, a driving assembly is arranged on the orbit assembly, a second box body is arranged on the driving assembly, a heating assembly is arranged outside the second box body, and a fixing assembly is arranged in the second box body; the heating assembly comprises a heating cavity which is arranged outside the second box body, the heating cavity is connected with a first hose and a second hose in a penetrating mode, an S-shaped bend pipe and an infrared heating plate are connected between the first hose and the second hose; and the fixing assembly comprises a fixing ring, transverse springs and longitudinal springs are arranged on the inner wall of the fixing ring. The medical clinical microorganism PCR detection device can avoid blowing bacteria during the heating process, improve the culture effect, and improve the precision of PCR detection results.

Description

Technical Field

[0001] This invention relates to the field of microbial PCR detection technology, and in particular to a medical clinical microbial PCR detection device. Background Technology

[0002] Tuberculosis (TB) is a chronic infectious disease caused by Mycobacterium tuberculosis. In the diagnosis of TB, PCR (polymerase chain reaction) is commonly used to detect Mycobacterium tuberculosis. PCR is a molecular biology technique used to amplify specific DNA fragments. It can be viewed as a special DNA replication process outside the organism. By amplifying the DNA of Mycobacterium tuberculosis, it facilitates the detection of the bacterium. The PCR process requires equipment such as a biochemical incubator, an electric drying oven, an electric constant temperature water bath, an autoclave, an ultra-low temperature freezer, and a centrifuge. And equipment such as constant temperature culture shakers, the patent number CN115478015A discloses a constant temperature shaker for biological cell culture and its usage method; the present invention includes a constant temperature shaker body, the ventilation ports at both ends of the constant temperature shaker body are provided with filters, the lower end face of the inner cavity wall of the constant temperature shaker body is slidably connected to the left and right sides and the front and rear ends of the inner cavity wall through sliding grooves with buffer plates, the lower end face of the lifting rod is fixedly connected with an anti-slip plate, the left and right ends of the constant temperature shaker body are rotatably connected to a turntable through a first rotating shaft, the upper end of the turntable is rotatably connected to a reciprocating rod through a pin shaft, the front end face of the reciprocating rod is fixedly connected with a fixing plate, and the close-to-each end faces of the fixing plates are provided with brushes.

[0003] However, existing microbial PCR detection devices using constant temperature incubators have some drawbacks, such as:

[0004] Existing microbial PCR detection devices, such as those mentioned above, use constant temperature incubators with heating rods directly placed inside the incubator body. Air flows directly into the incubator body, and heat is conducted through the air to raise the internal temperature of the incubator and achieve constant temperature. However, this can easily introduce bacteria from outside the environment into the incubator, affecting the incubator's ability to culture microbial PCR detection sample solutions. Therefore, we propose a medical clinical microbial PCR detection device. Summary of the Invention

[0005] In view of this, the purpose of this invention is to provide a medical clinical microbial PCR detection device to solve the problem that existing microbial PCR detection devices use constant temperature incubators, which can easily introduce bacteria from outside the environment into the incubator, affecting the incubator's ability to culture microbial PCR detection sample solutions.

[0006] Based on the above objectives, the present invention provides a medical clinical microbial PCR detection device, including a constant temperature culture shaker. The constant temperature culture shaker includes a device body and a control system. The device body includes a first housing. A track assembly is provided inside the first housing. A drive assembly is provided on the track assembly. A second housing is provided on the drive assembly. A heating assembly is provided outside the second housing. Three sets of fixing components are provided inside the second housing.

[0007] The heating assembly includes a heating cavity fitted outside the second housing. A first flexible tube and a second flexible tube are respectively connected through the lower parts of both sides of the heating cavity. An S-shaped bend is connected between the first flexible tube and the second flexible tube. An infrared heating plate is fitted outside the S-shaped bend. The infrared heating plate is installed inside the first housing. The S-shaped bend, the first flexible tube, the second flexible tube and the interior of the heating cavity are sealed and filled with liquid water.

[0008] The upper end of the second box is connected to the second box cover by a hinge. The three sets of fixing components are arranged in an array at equal intervals in the vertical direction. Each set of fixing components includes a fixing ring fixedly installed on the inner wall of the second box. The inner wall of the fixing ring is provided with eleven horizontal springs and eleven vertical springs. The eleven horizontal springs are located above the eleven vertical springs, and the eleven horizontal springs and the eleven vertical springs are perpendicular to each other. The distance between two adjacent horizontal springs increases by two centimeters in turn, and the distance between two adjacent vertical springs increases by two centimeters in turn.

[0009] Furthermore, a first lid is connected to the first box body via a hinge. An installation groove is provided on the upper part of the first box body near the hinge, and a push switch is installed in the installation groove. A protrusion is provided on the lower part of the first lid near the hinge, and the protrusion is compatible with the push switch.

[0010] Furthermore, both the first and second lids are made of transparent material, and both the first and second lids are equipped with handles. The bottom of the first box is equipped with support legs.

[0011] Furthermore, a temperature sensor is provided inside the second housing, and a circulation pump is provided at one end of the S-shaped bend, with the circulation pump located on one side of the infrared heating plate.

[0012] Furthermore, the track assembly includes a bottom rail disposed at the bottom of the first housing and four connecting strips disposed on the inner side wall of the first housing. The ends of the connecting strips are provided with connecting blocks, and two second tracks are disposed on the four connecting blocks. The bottom rail is located directly below the two second tracks. Track grooves are provided on the bottom rail. The two second tracks are arranged side by side in the vertical direction, with a distance of one centimeter between the two second tracks. The inner walls of the two second tracks are provided with teeth.

[0013] Furthermore, the driving assembly includes a first roller movably disposed inside the track groove. The first roller is horizontally positioned, and a support rod is movably disposed on the upper end of the first roller. A drive motor is disposed on the support rod, and a connecting shaft is disposed on the upper output end of the drive motor. Two gears are disposed on the connecting shaft, and the two gears mesh with the teeth of the inner walls of the two second tracks respectively. A rotating ring is sleeved on the connecting shaft, and the rotating ring is located between the two gears. A horizontal shaft is disposed on the side wall of the rotating ring, and the horizontal shaft is located between the two second tracks. A vertical shaft is disposed on the side of the horizontal shaft, and second rollers are connected to both ends of the vertical shaft through bearings. The two second rollers are respectively in contact with the outer walls of the two second tracks.

[0014] Furthermore, the horizontal axis is perpendicular to the connecting axis, the vertical axis is parallel to the connecting axis, and a third roller is connected to the middle of the horizontal axis via a bearing. The third roller is in contact with the opposing surfaces of the two second tracks.

[0015] Furthermore, the bottom of the second housing is connected to the upper end of the connecting shaft via a bearing, the inner wall of the first housing is provided with a horizontal plate, and the second housing is located above the horizontal plate.

[0016] Furthermore, a control module is provided inside the first housing, and a touch screen is provided outside the first housing. The control module is electrically connected to the push switch, the temperature sensor, the infrared heating plate, the drive motor, and the touch screen.

[0017] Furthermore, the control system includes a temperature control unit, a swing control unit, and a timing unit. The timing unit performs timing operations through a clock chip inside the control module. The temperature control unit first inputs the temperature range via the touchscreen, then the infrared heating plate starts working to heat the inside of the second chamber. The temperature sensor is used to obtain the internal temperature of the second chamber. If the temperature is greater than the maximum temperature value of the temperature range, heating stops; if it is less than the minimum temperature value of the temperature range, heating continues. The swing control unit first obtains the swing frequency n input via the touchscreen. The swing frequency n is the number of times the gear rotates around the inner wall of the second track in one minute. The swing control unit controls the speed of the drive motor through the swing frequency n. After the device is turned on, the first chamber cover is closed. The protrusion presses the push switch. When the first chamber cover is opened, the protrusion no longer presses the push switch. The push switch transmits a signal to the control module. After obtaining the signal, the swing control unit controls the drive motor to stop rotating, and the second chamber stops swinging.

[0018] Compared with the prior art, the present invention has the following beneficial effects:

[0019] In this invention, an infrared heating plate heats the liquid water inside the S-shaped bend. The liquid water, as a medium, is transmitted to the heating chamber through the first hose and then heats the second chamber. The medium water then returns to the S-shaped bend through the second hose for circulation, thereby continuously heating the second chamber, improving the heating effect, and preventing hot air from being blown into the second chamber, thus avoiding the introduction of bacteria.

[0020] In this invention, by setting up a track assembly and a drive assembly, the first roller is mainly used to move along a specific trajectory through a support rod and a drive motor. The drive motor is a servo motor. After the output end of the drive motor is working, it drives the connecting shaft to rotate. The connecting shaft drives two gears to rotate. The two gears mesh with the teeth on the inner walls of the two second tracks, so that the drive motor and the connecting shaft move along the inner walls of the two second tracks. Since the top of the connecting shaft and the bottom of the second housing are connected to the bottom of the second housing through a bearing, the second housing only moves and does not rotate during the rotation of the connecting shaft. During the movement of the connecting shaft along the inner walls of the second tracks, the horizontal axis moves between the two second tracks. Since a rotating ring is sleeved on the connecting shaft, the connecting shaft only drives the horizontal axis to move and does not drive the horizontal axis to rotate. The horizontal axis drives the vertical axis to move, so that the second roller moves around the outer walls of the two second tracks, so that the two gears are always meshed with the teeth on the inner walls of the two second tracks, ensuring that the swing of the second housing is more stable and improving the use effect of the constant temperature incubator shaker.

[0021] In this invention, by setting up a control system, the internal temperature range of the second chamber 4 can be controlled and a constant temperature can be maintained. At the same time, the swing frequency of the second chamber is precisely controllable, and the second chamber can stop swinging the moment the first chamber lid is opened, thereby improving the performance of the equipment. Attached Figure Description

[0022] Figure 1 This is a schematic diagram of the overall structure of a medical clinical microbial PCR detection device according to the present invention;

[0023] Figure 2 This is a schematic diagram of the front section structure of a medical clinical microbial PCR detection device according to the present invention;

[0024] Figure 3 For the present invention Figure 2 A magnified schematic diagram of the local structure at point A;

[0025] Figure 4 This is a schematic diagram of the overall structure of the track component in a medical clinical microbial PCR detection device of the present invention;

[0026] Figure 5 This is a schematic diagram of the overall structure connecting the track component and the drive component in a medical clinical microbial PCR detection device of the present invention;

[0027] Figure 6 For the present invention Figure 5 A magnified schematic diagram of the local structure at point B;

[0028] Figure 7 This is a top view schematic diagram of the connection between the track component and the drive component in a medical clinical microbial PCR detection device of the present invention;

[0029] Figure 8 For the present invention Figure 7 A schematic diagram of the cross-sectional structure at the CC section;

[0030] Figure 9 This is a schematic diagram of the overall structure of the driving component in a medical clinical microbial PCR detection device of the present invention;

[0031] Figure 10 This is a schematic diagram showing the connection between the second chamber, heating component, and fixing component in a medical clinical microbial PCR detection device of the present invention.

[0032] Figure 11 For the present invention Figure 10 Schematic diagram of the mid-section structure;

[0033] Figure 12 This is a schematic diagram of the connection structure of the fixation component in a medical clinical microbial PCR detection device of the present invention;

[0034] Figure 13 This is a schematic diagram of the overall structure of the spring in a medical clinical microbial PCR detection device of the present invention.

[0035] In the diagram: 1. First housing; 2. Track assembly; 3. Drive assembly; 4. Second housing; 5. Heating assembly; 6. Fixing assembly; 7. First cover; 8. Second cover; 9. Push switch; 10. Protrusion; 11. Temperature sensor; 12. Horizontal plate; 13. Control module; 14. Touch screen; 201. Bottom rail; 202. Connecting strip; 203. Connecting block; 204. Second rail; 205. Tooth; 301. First roller 302, Support rod; 303, Drive motor; 304, Connecting shaft; 305, Gear; 306, Rotating ring; 307, Horizontal shaft; 308, Vertical shaft; 309, Second roller; 310, Third roller; 501, Heating chamber; 502, First flexible hose; 503, Second flexible hose; 504, S-shaped bend; 505, Infrared heating plate; 506, Circulating pump; 601, Fixing ring; 602, Horizontal spring; 603, Vertical spring. Detailed Implementation

[0036] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail below with reference to specific embodiments.

[0037] It should be noted that, unless otherwise defined, the technical or scientific terms used in this invention should have the ordinary meaning understood by one of ordinary skill in the art to which this invention pertains. The terms "first," "second," and similar terms used in this invention do not indicate any order, quantity, or importance, but are merely used to distinguish different components. Terms such as "comprising" or "including" mean that the element or object preceding the word encompasses the elements or objects listed following the word and their equivalents, without excluding other elements or objects. Terms such as "connected" or "linked" are not limited to physical or mechanical connections, but can include electrical connections, whether direct or indirect. Terms such as "upper," "lower," "left," and "right" are used only to indicate relative positional relationships; when the absolute position of the described object changes, the relative positional relationship may also change accordingly.

[0038] Please refer to the following: Figures 1-13 ,in, Figure 1 This is a schematic diagram of the overall structure of a medical clinical microbial PCR detection device according to the present invention; Figure 2 This is a schematic diagram of the front section structure of a medical clinical microbial PCR detection device according to the present invention; Figure 3 For the present invention Figure 2 A magnified schematic diagram of the local structure at point A; Figure 4This is a schematic diagram of the overall structure of the track component in a medical clinical microbial PCR detection device of the present invention;

[0039] Figure 5 This is a schematic diagram of the overall structure connecting the track component and the drive component in a medical clinical microbial PCR detection device of the present invention; Figure 6 For the present invention Figure 5 A magnified schematic diagram of the local structure at point B; Figure 7 This is a top view schematic diagram of the connection between the track component and the drive component in a medical clinical microbial PCR detection device of the present invention; Figure 8 For the present invention Figure 7 A schematic diagram of the cross-sectional structure at the CC section; Figure 9 This is a schematic diagram of the overall structure of the driving component in a medical clinical microbial PCR detection device of the present invention; Figure 10 This is a schematic diagram showing the connection between the second chamber, heating component, and fixing component in a medical clinical microbial PCR detection device of the present invention. Figure 11 For the present invention Figure 10 Schematic diagram of the mid-section structure; Figure 12 This is a schematic diagram of the connection structure of the fixation component in a medical clinical microbial PCR detection device of the present invention; Figure 13 This is a schematic diagram of the overall structure of the spring in a medical clinical microbial PCR detection device of the present invention.

[0040] A medical clinical microbial PCR detection device includes a constant temperature culture shaker, which includes a constant temperature culture shaker device body and a control system. The constant temperature culture shaker device body includes a first box 1, a track assembly 2 inside the first box 1, a drive assembly 3 on the track assembly 2, a second box 4 on the drive assembly 3, a heating assembly 5 outside the second box 4, and three sets of fixing assemblies 6 inside the second box 4.

[0041] Among them, the driving component 3 moves around the track component 2 to drive the second box 4 to swing, the infrared heating component 5 is mainly used to provide a constant temperature for the second box 4, and the fixing component 6 is used to fix test tubes or petri dishes of different sizes.

[0042] The heating assembly 5 includes a heating cavity 501 sleeved outside the second housing 4. A first hose 502 and a second hose 503 are respectively connected to the lower parts of both sides of the heating cavity 501. An S-shaped bend 504 is connected between the first hose 502 and the second hose 503. An infrared heating plate 505 is sleeved outside the S-shaped bend 504. The infrared heating plate 505 is installed inside the first housing 1. The S-shaped bend 504, the first hose 502, the second hose 503 and the interior of the heating cavity 501 are sealed and filled with liquid water.

[0043] The infrared heating plate 505 works by utilizing the absorption of light by an object. Infrared heat transfer is radiative heat transfer, with energy transferred by electromagnetic waves. When far-infrared rays irradiate the object being heated, some rays are reflected back, while others penetrate. When the wavelength of the emitted far-infrared rays matches the absorption wavelength of the object being heated, the object absorbs the far-infrared rays. At this time, the molecules and atoms inside the object resonate, generating strong vibrations and rotations. These vibrations and rotations raise the object's temperature, achieving the heating purpose. Ultimately, this heats the liquid water inside the S-shaped bend 504. The liquid water, as a medium, is transferred through the first hose 502 to the heating chamber 501, and then heats the second chamber 4. The medium water then returns to the S-shaped bend 504 through the second hose 503 for circulation, continuously heating the second chamber 4 and improving the heating effect.

[0044] The upper end of the second box 4 is connected to the second box cover 8 by a hinge. Three sets of fixing components 6 are arranged in a vertical array at equal intervals. Each set of fixing components 6 includes a fixing ring 601 fixedly installed on the inner wall of the second box 4. The inner wall of the fixing ring 601 is provided with eleven horizontal springs 602 and eleven vertical springs 603. The eleven horizontal springs 602 are located above the eleven vertical springs 603, and the eleven horizontal springs 602 and the eleven vertical springs 603 are perpendicular to each other. The distance between two adjacent horizontal springs 602 increases by two centimeters in succession, and the distance between two adjacent vertical springs 603 increases by two centimeters in succession.

[0045] In practical use, because the distance between two adjacent horizontal springs 602 increases by two centimeters each time, and the distance between two adjacent vertical springs 603 increases by two centimeters each time, the vertical springs 603 and the horizontal springs 602 are staggered to form squares of different sizes. The horizontal springs 602 and vertical springs 603 used in this invention are both composed of lightweight springs, such as... Figure 13 As shown, it has a certain deformation capability, and the elastic force between the springs can fix test tubes and petri dishes of different sizes.

[0046] Furthermore, a first box cover 7 is connected to the first box body 1 by a hinge. The upper part of the first box body 1 near the hinge has an installation groove, in which a push switch 9 is installed. The lower part of the first box cover 7 near the hinge has a protrusion 10, which is compatible with the push switch 9. Both the first box cover 7 and the second box cover 8 are made of transparent material, and both the first box cover 7 and the second box cover 8 have handles. The bottom of the first box body 1 has support legs.

[0047] The support legs are mainly used to support the equipment. The handles make it easy to open the first cover 7 and the second cover 8. The first box 1 and the second box 4 are designed as a two-layer structure to prevent dust and impurities from entering the second box 4 and affecting the solution samples required for PCR detection. The first cover 7 and the second cover 8 are made of transparent material, which makes it easy to directly observe the condition of the test tubes or petri dishes in the second box 4 for constant temperature incubation of the sample solution. In actual use, when the first cover 7 is opened, the protrusion 10 no longer presses the button switch 9. Since the button switch 9 is located near the hinge, when the first cover 7 is lifted, the protrusion 10 can rise quickly, and the button switch 9 can quickly generate a signal.

[0048] Furthermore, a temperature sensor 11 is provided inside the second housing 4, and a circulation pump 506 is provided at one end of the S-shaped bend 504. The circulation pump 506 is located on one side of the infrared heating plate 505.

[0049] Temperature sensor 11 is mainly used to measure the temperature inside the second chamber 4. Infrared heating plate 505 wraps around S-shaped bend 504 from two directions to improve the heating effect of liquid water inside S-shaped bend 504. By setting circulation pump 506, liquid water is always circulated inside S-shaped bend 504, first hose 502, second hose 503 and heating chamber 501, which can improve the heating effect and ultimately improve the constant temperature heating effect of the second chamber 4.

[0050] Furthermore, the track assembly 2 includes a bottom rail 201 disposed at the bottom of the first housing 1 and four connecting strips 202 disposed on the inner side wall of the first housing 1. The ends of the connecting strips 202 are provided with connecting blocks 203. Two second rails 204 are provided on the four connecting blocks 203. The bottom rail 201 is located directly below the two second rails 204. The bottom rail 201 is provided with a track groove. The two second rails 204 are arranged side by side in the vertical direction. The distance between the two second rails 204 is one centimeter. The inner wall of the two second rails 204 is provided with teeth 205.

[0051] Among them, such as Figure 4 As shown, the bottom rail 201 and the two second rails 204 are in the shape of a cross and a plum blossom. Under the drive of the drive component 3, the second box 4 slides quickly along the trajectory of the two second rails 204 to achieve the purpose of swinging. The connecting strip 202 and the connecting block 203 are mainly used to fix the second rails 204.

[0052] Furthermore, the drive assembly 3 includes a first roller 301 movably disposed inside the track groove. The first roller 301 is horizontally disposed. A support rod 302 is movably disposed on the upper end of the first roller 301. A drive motor 303 is disposed on the support rod 302. A connecting shaft 304 is disposed on the upper output end of the drive motor 303. Two gears 305 are disposed on the connecting shaft 304. The two gears 305 mesh with the teeth 205 on the inner wall of the two second tracks 204 respectively. A rotating ring 306 is sleeved on the connecting shaft 304. The rotating ring 306 is located between the two gears 305. A horizontal shaft 307 is disposed on the side wall of the rotating ring 306. The horizontal shaft 307 is located between the two second tracks 204. A vertical shaft 308 is disposed on the side of the horizontal shaft 307. The two ends of the vertical shaft 308 are connected to second rollers 309 through bearings. The two second rollers 309 are respectively in contact with the outer wall of the two second tracks 204.

[0053] The first roller 301 is mainly used to move along a specific trajectory via the support rod 302 and the drive motor 303. The drive motor 303 is a servo motor. After the output end of the drive motor 303 is working, it drives the connecting shaft 304 to rotate. The connecting shaft 304 drives the two gears 305 to rotate. The two gears 305 mesh with the teeth 205 on the inner wall of the two second tracks 204, so that the drive motor 303 and the connecting shaft 304 move along the inner wall of the two second tracks 204. Since the top of the connecting shaft 304 is connected to the bottom of the second housing 4 through a bearing, the connecting shaft 304 rotates. During the movement, the second housing 4 moves but does not rotate. As the connecting shaft 304 moves along the inner wall of the second track 204, the horizontal shaft 307 moves between the two second tracks 204. Since the connecting shaft 304 is fitted with a rotating ring 306, the connecting shaft 304 only drives the horizontal shaft 307 to move, without rotating it. The horizontal shaft 307 drives the vertical shaft 308 to move, causing the second roller 309 to move around the outer wall of the two second tracks 204. This ensures that the two gears 305 are always meshed with the teeth 205 on the inner wall of the two second tracks 204, guaranteeing the swing of the second housing 4.

[0054] Furthermore, the horizontal axis 307 is perpendicular to the connecting axis 304, and the vertical axis 308 is parallel to the connecting axis 304. A third roller 310 is connected to the middle of the horizontal axis 307 via a bearing, and the third roller 310 is in contact with the opposing surfaces of the two second tracks 204.

[0055] During the movement of the horizontal axis 307, the third roller 310 rolls on the opposite surfaces of the two second tracks 204 to assist the movement of the horizontal axis 307 and improve the stability of the swing of the second box 4.

[0056] Furthermore, the bottom of the second housing 4 is connected to the upper end of the connecting shaft 304 via a bearing, and the inner wall of the first housing 1 is provided with a horizontal plate 12, with the second housing 4 located above the horizontal plate 12.

[0057] The horizontal plate 12 is mainly used to prevent impurities or dust from entering the track assembly 2 and the drive assembly 3, so as to ensure the precision of the operation of the track assembly 2 and the drive assembly 3.

[0058] Furthermore, the first housing 1 is equipped with a control module 13 inside and a touch screen 14 outside the first housing 1. The control module 13 is electrically connected to the push switch 9, the temperature sensor 11, the infrared heating plate 505, the drive motor 303 and the touch screen 14.

[0059] The control module 13 contains a control chip and a microcontroller. The connection of the control circuit is common knowledge and will not be described in detail here. Since the drive motor 303 is a servo motor, when the output of the drive motor 303 stops rotating, the gear 305 stops rotating. Under the meshing of the teeth 205, the gear 305 will immediately stop rotating, so that the second box 4 stops rotating. Through the meshing of the gear 305 and the teeth 205, braking is achieved, so that the second box 4 stops swinging the moment the first box cover 7 is opened, thus improving the use effect of the equipment.

[0060] Furthermore, the control system includes a temperature control unit, a swing control unit, and a timing unit. The timing unit performs timing operations through the clock chip inside the control module 13. The temperature control unit first inputs the temperature range through the touch screen 14, and then the infrared heating plate 505 starts working to heat the inside of the second chamber 4. The temperature sensor 11 is used to obtain the internal temperature of the second chamber 4. If the temperature is greater than the maximum temperature value of the temperature range, heating stops; if the temperature is less than the minimum temperature value of the temperature range, heating continues. The swing control unit first obtains the swing frequency n input by the touch screen 14. The swing frequency n is the number of times the gear 305 moves around the inner wall of the second track 204 once per minute. The swing control unit controls the speed of the drive motor 303 through the swing frequency n. After the equipment is opened, the first chamber cover 7 is closed, and the protrusion 10 presses the push switch 9. When the first chamber cover 7 is opened, the protrusion 10 no longer presses the push switch 9. The push switch 9 transmits a signal to the control module 13. After the swing control unit obtains the signal, it controls the drive motor 303 to stop rotating, and the second chamber 4 stops swinging.

[0061] By setting up a control system, the internal temperature range of the second chamber 4 can be controlled and kept constant. At the same time, the swing frequency of the second chamber 4 can be precisely controlled, and the second chamber 4 can stop swinging the moment the first chamber cover 7 is opened, thus improving the performance of the equipment.

[0062] In summary, in actual use, first open the first box cover 7 and the second box cover 8, and place the test tubes or petri dishes containing the sample reagents required for medical clinical microbial PCR detection into the grid formed by the alternating vertical springs 603 and horizontal springs 602. Simultaneously, select a grid of appropriate size according to the size of the test tubes or petri dishes. The elasticity between the springs can fix test tubes and petri dishes of different sizes. Close the first box cover 7 and the second box cover 8, and input the temperature range and the swing frequency n of the second box 4 via the touch screen 14 to start the device. The heating operation begins first, with the infrared heating plate 505 heating the liquid water inside the S-shaped bend 504. This liquid water serves as the medium flowing through the first flexible tube. 502 is transmitted to the heating chamber 501, and then heats the second chamber 4. The medium water returns to the S-shaped bend 504 through the second hose 503 for circulation, continuously heating the second chamber 4. The temperature sensor 11 acquires the internal temperature of the second chamber 4. If it is higher than the maximum temperature value of the temperature range, heating stops; if it is lower than the minimum temperature value of the temperature range, heating continues to maintain a constant temperature inside the second chamber 4. After the output end of the drive motor 303 is working, it drives the connecting shaft 304 to rotate. The connecting shaft 304 drives the two gears 305 to rotate. The two gears 305 mesh with the teeth 205 on the inner wall of the two second tracks 204, so that the drive motor 303 and the connecting shaft 304... Moving along the inner walls of the two second tracks 204, the top of the connecting shaft 304 is connected to the bottom of the second housing 4 via a bearing, so that during the rotation of the connecting shaft 304, the second housing 4 only moves and does not rotate. As the connecting shaft 304 moves along the inner walls of the second tracks 204, the horizontal shaft 307 moves between the two second tracks 204. Because a rotating ring 306 is fitted on the connecting shaft 304, the connecting shaft 304 only drives the horizontal shaft 307 to move, not to rotate it. The horizontal shaft 307 drives the vertical shaft 308 to move, causing the second roller 309 to move around the outer walls of the two second tracks 204, so that the two gears 305 respectively engage with the teeth 2 on the inner walls of the two second tracks 204. 05 is always engaged to ensure the swing of the second housing 4. During the rotation and movement of the connecting shaft 304, the first roller 301 moves along the corresponding trajectory through the support rod 302 and the drive motor 303. When the set time is reached, the output end of the drive motor 303 stops rotating and the second housing 4 stops swinging. Alternatively, when the first housing cover 7 is opened, the protrusion 10 stops pressing the button switch 9. The button switch 9 transmits a signal to the control module 13. After the swing control unit receives the signal, it controls the drive motor 303 to stop rotating and the second housing 4 stops swinging. The test tube or culture dish containing the sample reagents required for medical clinical microbial PCR detection is taken out to carry out the next step of PCR detection and complete the diagnosis of tuberculosis.

[0063] Those skilled in the art should understand that the discussion of any of the above embodiments is merely exemplary and is not intended to imply that the scope of the invention (including the claims) is limited to these examples; within the framework of the invention, the technical features of the above embodiments or different embodiments can also be combined, the steps can be implemented in any order, and there are many other variations of the different aspects of the invention as described above, which are not provided in the details for the sake of brevity.

[0064] This invention is intended to cover all such substitutions, modifications, and variations that fall within the broad scope of the appended claims. Therefore, any omissions, modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this invention should be included within the scope of protection of this invention.

Claims

1. A medical clinical microbial PCR detection device, comprising a constant temperature incubator shaker, characterized in that: The constant temperature culture shaker includes a constant temperature culture shaker device body and a control system. The constant temperature culture shaker device body includes a first box (1), a track assembly (2) is provided inside the first box (1), a drive assembly (3) is provided on the track assembly (2), a second box (4) is provided on the drive assembly (3), a heating assembly (5) is provided outside the second box (4), and three sets of fixing assemblies (6) are provided inside the second box (4). The track assembly (2) includes a bottom rail (201) disposed at the bottom of the first housing (1) and four connecting strips (202) disposed on the inner side wall of the first housing (1). The ends of the connecting strips (202) are provided with connecting blocks (203). Two second tracks (204) are provided on the four connecting blocks (203). The bottom rail (201) is located directly below the two second tracks (204). The bottom rail (201) is provided with track grooves. The two second tracks (204) are arranged side by side in the vertical direction. The distance between the two second tracks (204) is one centimeter. The inner walls of the two second tracks (204) are provided with teeth (205). The bottom rail (201) and the two second tracks (204) are arranged in a cross-shaped plum blossom pattern. The heating assembly (5) includes a heating chamber (501) sleeved outside the second housing (4). The lower sides of the heating chamber (501) are respectively connected to a first hose (502) and a second hose (503). An S-shaped bend (504) is connected between the first hose (502) and the second hose (503). An infrared heating plate (505) is sleeved outside the S-shaped bend (504). The infrared heating plate (505) is installed inside the first housing (1). The S-shaped bend (504), the first hose (502), the second hose (503) and the heating chamber (501) are sealed and filled with liquid water. The upper end of the second box (4) is connected to the second box cover (8) by a hinge. The three sets of fixing components (6) are arranged in an array at equal intervals in the vertical direction. Each set of fixing components (6) includes a fixing ring (601) fixedly installed on the inner wall of the second box (4). The inner wall of the fixing ring (601) is provided with eleven horizontal springs (602) and eleven vertical springs (603). The eleven horizontal springs (602) are located on the upper end of the eleven vertical springs (603), and the eleven horizontal springs (602) and the eleven vertical springs (603) are perpendicular to each other. The distance between two adjacent horizontal springs (602) increases by two centimeters in sequence, and the distance between two adjacent vertical springs (603) increases by two centimeters in sequence.

2. The medical clinical microbial PCR detection device according to claim 1, characterized in that: The first box body (1) is connected to the first box cover (7) by a hinge. The upper part of the first box body (1) near the hinge is provided with an installation groove, and a push switch (9) is installed in the installation groove. The lower part of the first box cover (7) near the hinge is provided with a protrusion (10), and the protrusion (10) is compatible with the push switch (9).

3. The medical clinical microbial PCR detection device according to claim 2, characterized in that: Both the first box cover (7) and the second box cover (8) are made of transparent material, and both the first box cover (7) and the second box cover (8) are provided with handles. The bottom of the first box body (1) is provided with support legs.

4. The medical clinical microbial PCR detection device according to claim 3, characterized in that: The second housing (4) is equipped with a temperature sensor (11), and a circulation pump (506) is provided on one end of the S-shaped bend (504). The circulation pump (506) is located on one side of the infrared heating plate (505).

5. The medical clinical microbial PCR detection device according to claim 4, characterized in that: The drive assembly (3) includes a first roller (301) movably disposed inside the track groove. The first roller (301) is horizontally disposed, and a support rod (302) is movably disposed on the upper end of the first roller (301). A drive motor (303) is disposed on the support rod (302), and a connecting shaft (304) is disposed on the upper output end of the drive motor (303). Two gears (305) are disposed on the connecting shaft (304), and the two gears (305) respectively engage with the teeth (205) on the inner wall of the two second tracks (204). The gears mesh with each other. A rotating ring (306) is fitted on the connecting shaft (304). The rotating ring (306) is located between the two gears (305). A horizontal shaft (307) is provided on the side wall of the rotating ring (306). The horizontal shaft (307) is located between the two second tracks (204). A vertical shaft (308) is provided on the side of the horizontal shaft (307). The two ends of the vertical shaft (308) are connected to second rollers (309) through bearings. The two second rollers (309) are respectively in contact with the outer walls of the two second tracks (204).

6. The medical clinical microbial PCR detection device according to claim 5, characterized in that: The horizontal axis (307) is perpendicular to the connecting axis (304), the vertical axis (308) is parallel to the connecting axis (304), and a third roller (310) is connected to the middle of the horizontal axis (307) through a bearing. The third roller (310) is in contact with the opposite surfaces of the two second tracks (204).

7. The medical clinical microbial PCR detection device according to claim 5, characterized in that: The bottom of the second box (4) is connected to the upper end of the connecting shaft (304) via a bearing. The inner wall of the first box (1) is provided with a horizontal plate (12), and the second box (4) is located above the horizontal plate (12).

8. The medical clinical microbial PCR detection device according to claim 5, characterized in that: The first housing (1) is equipped with a control module (13) inside and a touch screen (14) is equipped on the outside of the first housing (1). The control module (13) is electrically connected to the push switch (9), the temperature sensor (11), the infrared heating plate (505), the drive motor (303) and the touch screen (14).

9. A medical clinical microbial PCR detection device according to claim 5, characterized in that: The control system includes a temperature control unit, a swing control unit, and a timing unit. The timing unit performs timing operations through a clock chip inside the control module (13). The temperature control unit first inputs the temperature range through the touch screen (14), and then the infrared heating plate (505) starts working to heat the inside of the second box (4). The temperature sensor (11) is used to obtain the temperature inside the second box (4). If the temperature is greater than the maximum temperature value of the temperature range, heating stops; if the temperature is less than the minimum temperature value of the temperature range, heating continues. The swing control unit first obtains the temperature range input through the touch screen (14). The swing frequency n is the number of times the gear (305) moves around the inner wall of the second track (204) once per minute. The swing control unit controls the speed of the drive motor (303) through the swing frequency n. After the device is opened, the first box cover (7) is closed. The protrusion (10) presses the push switch (9). When the first box cover (7) is opened, the protrusion (10) no longer presses the push switch (9). The push switch (9) transmits the signal to the control module (13). After the swing control unit obtains the signal, it controls the drive motor (303) to stop rotating, and the second box (4) no longer swings.

Images