An automated dispensing apparatus and a pull plating method using the same

Abstract

The application discloses an automatic liquid distribution device, which comprises a base, a controller, an X-axis mechanical arm and a Y-axis mechanical arm. The vertical part of the base is provided with an X-axis sliding rail in the transverse direction. The Y-axis mechanical arm is in the shape of a shell with an open lower end. The Y-axis mechanical arm is provided with a Z-axis sliding rail and a multi-channel automatic liquid moving head. The multi-channel automatic liquid moving head is slidably arranged on the Z-axis sliding rail and is driven by a Z-axis lifting cylinder. The controller is installed in the vertical part of the base. The controller is connected with a touch screen. The automatic liquid distribution device can automatically distribute the outer membrane solution and effectively reduce the residual amount of the outer membrane solution in the liquid suction head. The application also discloses a pulling and plating method. The pulling and plating method uses a pulling and plating machine and the automatic liquid distribution device. The pulling and plating method can keep the consistency of the height of the outer membrane after pulling and dipping, make the surface of the outer membrane free of bubbles, form good compactness, reduce the use amount of the outer membrane solution, reduce the cost and improve the efficiency of the pulling and plating.

Description

Technical Field

[0001] This invention relates to an automated liquid separation device and a lifting coating method using the device. Background Technology

[0002] Diabetes is a common chronic disease. Its danger lies not in diabetes itself, but in the complications arising from abnormal blood sugar levels. Studies have shown that monitoring and controlling blood sugar can effectively reduce the risk of these complications. In continuous glucose monitoring systems, the glucose sensor is crucial, and its outer membrane determines the overall performance and detection results.

[0003] The outer membrane of the sensor is prepared using the dip-coating method, which significantly improves production efficiency. However, the outer membrane solution evaporates continuously with increasing dip-coating time, and uneven liquid separation heights can occur due to human error, such as uneven liquid aspiration by the experimenter. Furthermore, the high concentration of the outer membrane solution can lead to excessive waste due to excessive residue in the pipette tip. Additionally, manual liquid separation can result in air pockets in the sample well, causing pores on the membrane surface formed by dip-coating and resulting in poor membrane density. Summary of the Invention

[0004] The purpose of this invention is to overcome the shortcomings of the prior art and provide an automated dispensing device that can automatically dispense outer membrane solutions and effectively reduce the residual amount of inner and outer membrane solutions on pipette tips.

[0005] Another objective of this invention is to provide a dip coating method that uses a dip coating machine and the aforementioned automated liquid separation device to perform dip coating, thereby maintaining the consistency of the outer film height after dip immersion, ensuring that the outer film surface is free of bubbles, forming good density, reducing the amount of outer film solution used, reducing costs, and improving the efficiency of dip coating.

[0006] One technical solution to achieve the above objective is: an automated liquid dispensing device, comprising a base, a controller, an X-axis robotic arm, and a Y-axis robotic arm, wherein:

[0007] The base is L-shaped, consisting of a horizontal section and a vertical section. The horizontal section of the base is provided with a pipette tip placement area, a sample area, a liquid storage area, a spare area, and a waste pipette tip storage area. The pipette tip placement area is provided with a pipette tip placement slot, the sample area is provided with a sample slot, the liquid storage area is provided with a liquid storage slot, the waste pipette tip storage area is provided with a waste pipette tip storage slot, and the spare area is used to temporarily store the pipette tip placement slot, the sample slot, or the liquid storage slot.

[0008] An X-axis slide rail is horizontally arranged on the vertical part of the base, and the rear end of the X-axis robotic arm is slidably mounted on the X-axis slide rail and driven by the X-axis drive mechanism.

[0009] A Y-axis slide rail is longitudinally arranged on one side of the X-axis robotic arm;

[0010] The Y-axis robotic arm can be slidably mounted on the Y-axis slide rail and driven by the Y-axis drive mechanism;

[0011] The Y-axis robotic arm has a shell-like structure with an open bottom. Inside the Y-axis robotic arm are a Z-axis slide rail and a multi-channel automatic pipetting head. The multi-channel automatic pipetting head can be slidably mounted on the Z-axis slide rail and driven by a Z-axis lifting cylinder.

[0012] The controller is installed in the vertical part of the base, and the X-axis drive mechanism, Y-axis drive mechanism, Z-axis lifting cylinder and multi-channel automatic pipette are respectively connected to the controller. The controller is connected to a touch screen.

[0013] In the aforementioned automated liquid dispensing device, the sample area and the pipette tip placement area are distributed one in front of the other on the left side of the horizontal portion of the base, the spare area and the liquid storage area are distributed one in front of the other in the middle of the horizontal portion of the base, and the waste pipette tip storage area is distributed on the right side of the horizontal portion of the base.

[0014] In the aforementioned automated liquid dispensing device, the pipette tip placement slot and the sample slot each have multiple small compartments, and the liquid storage tank is provided with multiple elongated compartments.

[0015] In the aforementioned automated liquid separation device, the sample area is connected to the immersion and lifting station of the lifting coating machine via a conveyor belt, and the conveyor belt is used for the sample tank to travel back and forth between the sample area and the immersion and lifting station.

[0016] In the aforementioned automated liquid dispensing device, the X-axis drive mechanism and the Y-axis drive mechanism are respectively adopted as stepper motors or cylinders.

[0017] In the aforementioned automated liquid dispensing device, the multi-channel automatic pipette employs a one-suction-multiple-spray method for liquid aspiration and dispensing.

[0018] In the aforementioned automated liquid dispensing device, the multi-channel automatic pipette is detachably mounted on the Z-axis slide rail via a connector.

[0019] This invention also provides a dip coating method, which uses a dip coating machine and the aforementioned automated liquid separation device to perform dip coating, specifically including the following steps:

[0020] S1. Prepare multiple pipette tips and place them into the pipette tip storage slots respectively;

[0021] S2, Prepare the outer membrane solution for lifting and pour it into the storage tank;

[0022] S3, set the dispensing parameters on the touch screen. The dispensing parameters include the amount of liquid aspirated by the multi-channel automatic pipette and the number of times the liquid is dispensed. Then start the automatic dispensing device.

[0023] S4, the controller controls the X-axis drive mechanism and the Y-axis drive mechanism to move the X-axis robotic arm and the Y-axis robotic arm in a one-to-one correspondence, so that the Y-axis robotic arm is exactly above the pipette tip placement slot. Then the controller controls the Z-axis lifting cylinder to drive the multi-channel automatic pipette to lower, so that each channel of the multi-channel automatic pipette is connected to a pipette tip. Then the controller controls the Z-axis lifting cylinder to drive the multi-channel automatic pipette to raise.

[0024] S5, the controller controls the X-axis drive mechanism and the Y-axis drive mechanism to move the X-axis robotic arm and the Y-axis robotic arm in a one-to-one correspondence, so that the Y-axis robotic arm moves to the top of the liquid storage tank. Then the controller controls the Z-axis lifting cylinder to drive the multi-channel automatic pipette to lower. The pipette tip is inserted into the outer membrane solution. The controller controls the multi-channel automatic pipette to aspirate liquid according to the preset dispensing parameters. Each pipette tip aspirates the outer membrane solution at the same time. Then the controller controls the Z-axis lifting cylinder to drive the multi-channel automatic pipette to rise.

[0025] S6, the controller controls the X-axis drive mechanism and the Y-axis drive mechanism to move the X-axis robotic arm and the Y-axis robotic arm in a one-to-one correspondence, so that the Y-axis robotic arm moves to the sample cell. Then, the controller controls the Z-axis lifting cylinder to drive the multi-channel automatic pipette to lower, so that the bottom of each pipette tip is located in the small compartment of the corresponding sample cell. The controller controls the multi-channel automatic pipette to discharge liquid through multiple sprays according to the preset dispensing parameters, so that the outer membrane solution in the pipette tip is fully and completely injected into the small compartment of the corresponding sample cell.

[0026] S7. After the drainage action is completed, the controller controls the Z-axis lifting cylinder to lift the multi-channel automatic pipette and controls the X-axis drive mechanism and Y-axis drive mechanism to move the X-axis robotic arm and Y-axis robotic arm in a corresponding manner, so that the Y-axis robotic arm moves above the waste pipette tip storage tank. The controller then controls the multi-channel automatic pipette to remove the used pipette tips.

[0027] S8, the sample tank after separation is moved by conveyor belt to the immersion and lifting station of the lifting coating machine to start the lifting coating.

[0028] In the above-mentioned lifting coating method, when a single lifting coating machine is used for multiple immersion lifting coatings, during the interval between two immersion lifting waiting times, the sample is returned to the sample area of ​​the automated liquid separation device via a conveyor belt to repeat steps S1-S8.

[0029] When multiple lifting coating machines are operated simultaneously, the immersion lifting station of each lifting coating machine and the sample area of ​​the automated liquid separation device are connected by a ring conveyor belt. The sample tank is then transported to the designated immersion lifting station for lifting coating. During the interval between two immersion lifting waits, the sample tank is transported to the sample area of ​​the automated liquid separation device using the ring conveyor belt, and steps S1-S8 are repeated.

[0030] In the above-mentioned lifting coating method, in step S6, after the first liquid spraying is completed, each subsequent liquid spraying is performed by the controller first lifting the multi-channel automatic pipette via the Z-axis lifting cylinder, raising the position of the pipette tip above the sample tank, so that the air in the pipette tip is discharged outwards first. Then, the controller lowers the multi-channel automatic pipette via the Z-axis lifting cylinder, so that the outer film solution in the pipette tip flows into the sample tank.

[0031] The automated dispensing device of this invention can automatically dispense the outer membrane solution, effectively reducing the residual amount of the inner and outer membrane solution in the pipette tip. The lifting coating method of this invention can maintain the consistency of the outer membrane height after lifting and immersion, making the outer membrane surface free of bubbles, forming good density, reducing the amount of outer membrane solution used, reducing costs, and improving the efficiency of lifting coating. Attached Figure Description

[0032] Figure 1 This is a structural diagram of an automated liquid dispensing device according to the present invention;

[0033] Figure 2 A schematic diagram of the horizontal sections of the base of the automated liquid dispensing device;

[0034] Figure 3 This is the electrical schematic diagram of an automated liquid separation device;

[0035] Figure 4 This is a schematic diagram showing the connection between a single lift coating machine and an automated liquid separation device;

[0036] Figure 5 This is a schematic diagram showing the connection between multiple lift coating machines and an automated liquid separation device. Detailed Implementation

[0037] To enable those skilled in the art to better understand the technical solution of the present invention, its specific embodiments are described in detail below with reference to the accompanying drawings:

[0038] Please see Figure 1 , Figure 2 and Figure 3 According to an embodiment of the present invention, an automated liquid dispensing device includes a base 1, a controller 8, an X-axis robotic arm 2, and a Y-axis robotic arm 3.

[0039] The base 1 is L-shaped, consisting of a horizontal section and a vertical section. The horizontal section of the base 1 includes a pipette tip placement area 11, a sample area 12, a liquid storage area 13, a spare area 14, and a waste pipette tip storage area 15. The sample area 12 and the pipette tip placement area 11 are located one after the other on the left side of the horizontal section of the base 1. The spare area 14 and the liquid storage area 13 are located one after the other in the middle of the horizontal section of the base 1. The waste pipette tip storage area 15 is located on the right side of the horizontal section of the base 1. The pipette tip placement area 11 has a pipette tip placement slot 4, the sample area 12 has a sample slot 5, the liquid storage area 13 has a liquid storage tank 6, and the waste pipette tip storage area 15 has a waste pipette tip storage slot 7. The spare area 14 is used to temporarily store the pipette tip placement slot, sample slot, or liquid storage tank. The pipette tip placement slot 4 and the sample slot 5 each have multiple small compartments, and the liquid storage tank 6 has multiple elongated compartments.

[0040] An X-axis slide rail 21 is horizontally arranged on the vertical part of the base 1. The rear end of the X-axis robotic arm 2 is slidably mounted on the X-axis slide rail 21 and driven by the X-axis drive mechanism 22. The X-axis drive mechanism 22 can be a stepper motor or a cylinder and is located inside the vertical part of the base.

[0041] The X-axis robotic arm 2 has a Y-axis slide rail 31 longitudinally arranged on one side; the Y-axis robotic arm 3 can be slidably arranged on the Y-axis slide rail 31 and driven by the Y-axis drive mechanism 32. The Y-axis drive mechanism 32 can be a stepper motor or a cylinder, and the X-axis drive mechanism 32 can be arranged inside the X-axis robotic arm 2.

[0042] The Y-axis robotic arm 3 has a shell-like structure with an open bottom. Inside the Y-axis robotic arm 3 is a Z-axis slide rail 34 and a multi-channel automatic pipetting head 9. The multi-channel automatic pipetting head 9 can be slidably mounted on the Z-axis slide rail 34 and driven by the Z-axis lifting cylinder 33.

[0043] The controller 8 is installed in the vertical part of the base 1, and the X-axis drive mechanism 22, Y-axis drive mechanism 32, Z-axis lifting cylinder 33 and multi-channel automatic pipette 9 are respectively connected to the controller 8. The controller 8 is connected to a touch screen 10.

[0044] The multi-channel automatic pipette 9 is detachably mounted on the Z-axis slide rail 34 via a connector. The multi-channel automatic pipette 9 is a commercially available product, such as an 8-channel or 96-channel automatic pipette, and is replaceable. The multi-channel automatic pipette 9 has multiple channels arranged in a matrix for connecting pipette tips. The working principle of the multi-channel automatic pipette is as follows: the multi-channel automatic pipette has a suction state and a discharge state. When the multi-channel automatic pipette is in the suction state, the pipette tip is above the reservoir 6. The position sensor on the multi-channel automatic pipette 9 sends a first detection signal to the controller. The controller generates a first operating control signal based on the received first detection signal and sends it to the drive component of the multi-channel automatic pipette 9. The drive component responds to the first operating control signal in a positive direction. When the multichannel automatic pipette 9 is in operation, the piston inside moves upward, drawing the reagent to be transferred into the pipette tip. When the multichannel automatic pipette 9 is in the dispensing state, the pipette tip is above the sample slot 5. The position sensor on the multichannel automatic pipette 9 sends a second detection signal to the controller. The controller generates a second operating control signal based on the received second detection signal and sends it to the drive assembly of the multichannel automatic pipette 9. The drive assembly runs in reverse in response to the second operating control signal, causing the piston inside the multichannel automatic pipette 9 to move downward and dispensing the reagent from the pipette tip.

[0045] Because the outer membrane solution has a high concentration and is quite viscous, the multi-channel automatic pipette 9 uses a one-suction-multiple-spray method for liquid aspiration and dispensing.

[0046] In the automated liquid dispensing device of the present invention, during use, the controller 8 drives the X-axis robotic arm 2 to slide left and right along the X-axis slide rail 21 via the X-axis drive mechanism 22, thereby realizing the left and right movement of the Y-axis robotic arm 3 and the multi-channel automatic pipette 9 within it; the controller 8 drives the Y-axis robotic arm 3 to move back and forth along the Y-axis slide rail 31 via the Y-axis drive mechanism 32, thereby realizing the back and forth movement of the multi-channel automatic pipette 9, and the multi-channel automatic pipette 9 is driven to rise and fall along the Z-axis slide rail 34 via the Z-axis lifting cylinder 33. Thus, the XYZ three-axis movement of the multi-channel automatic pipette 9 is realized. In actual operation, the specific position of the multi-channel automatic pipette 9 is monitored by a photoelectric sensor.

[0047] Please see again Figure 4 In the automated liquid separation device of the present invention, the sample area 12 is connected to the immersion lifting station 201 of the lifting coating machine 200 via a conveyor belt 100. The conveyor belt 100 is used for the sample tank 5 to travel back and forth between the sample area 12 and the immersion lifting station 201.

[0048] A dip coating method, employing a dip coating machine and the aforementioned automated liquid separation device, specifically includes the following steps:

[0049] S1. Prepare multiple pipette tips and place them into the pipette tip placement slot 4 respectively;

[0050] S2, prepare the outer membrane solution for lifting and pour it into the storage tank 6;

[0051] S3. Set the dispensing parameters on the touch screen 10. The dispensing parameters include the aspiration volume of the multi-channel automatic pipette and the number of times the liquid is dispensed. Then start the automatic dispensing device. Alternatively, the automatic dispensing device can be connected to a PC and the dispensing parameters can be set through the PC.

[0052] S4, the controller 8 controls the X-axis drive mechanism 22 and the Y-axis drive mechanism 32 to move the X-axis robotic arm 2 and the Y-axis robotic arm 3 in a one-to-one correspondence, so that the Y-axis robotic arm 3 is exactly above the pipette tip placement slot 4. Then the controller 8 controls the Z-axis lifting cylinder 33 to drive the multi-channel automatic pipette 9 to lower, so that each channel of the multi-channel automatic pipette 9 is connected to a pipette tip. Then the controller 8 controls the Z-axis lifting cylinder 33 to drive the multi-channel automatic pipette 9 to rise.

[0053] S5, the controller 8 controls the X-axis drive mechanism 22 and the Y-axis drive mechanism 32 to move the X-axis robotic arm 2 and the Y-axis robotic arm 3 in a one-to-one correspondence, so that the Y-axis robotic arm 3 moves above the liquid storage tank 6. Then the controller 9 controls the Z-axis lifting cylinder 33 to drive the multi-channel automatic pipette 9 to lower, and the pipette tip is inserted into the outer membrane solution. The controller 8 controls the multi-channel automatic pipette 9 to aspirate liquid according to the preset dispensing parameters. Each pipette tip aspirates the outer membrane solution at the same time. Then the controller 8 controls the Z-axis lifting cylinder 33 to drive the multi-channel automatic pipette 9 to rise.

[0054] S6, the controller 9 controls the X-axis drive mechanism 22 and the Y-axis drive mechanism 32 to move the X-axis robotic arm 2 and the Y-axis robotic arm 3 in a one-to-one correspondence, so that the Y-axis robotic arm 3 moves to the sample cell 5. Then, the controller 9 controls the Z-axis lifting cylinder 33 to drive the multi-channel automatic pipette 9 to lower, so that the bottom of each pipette tip is located in the small compartment 5 of the corresponding sample cell. The controller 8 controls the multi-channel automatic pipette 9 to discharge liquid through multiple sprays according to the preset dispensing parameters, so that the outer membrane solution in the pipette tip is fully and completely injected into the small compartment 5 of the corresponding sample cell. Inside; after the first spray, each subsequent spray is performed by the controller 9 first raising the multi-channel automatic pipette 9 via the Z-axis lifting cylinder 33, raising the pipette tip position above the sample trough 5, allowing the air inside the pipette tip to be expelled outwards. Then, the controller 9 lowers the multi-channel automatic pipette 9 via the Z-axis lifting cylinder 33, allowing the outer membrane solution inside the pipette tip to flow into the sample trough 5. This prevents excess air from impacting the existing outer membrane solution in the sample trough 5, thus minimizing the amount of gas that may be mixed into the outer membrane solution while draining as much of the outer membrane solution from the pipette tip as possible.

[0055] S7. After the drainage action is completed, the controller 9 controls the Z-axis lifting cylinder 33 to lift the multi-channel automatic pipette 9, and controls the X-axis drive mechanism 22 and the Y-axis drive mechanism 32 to move the X-axis robotic arm 2 and the Y-axis robotic arm 3 in a one-to-one correspondence, so that the Y-axis robotic arm 3 moves above the waste pipette tip storage tank 7, and the controller 9 controls the multi-channel automatic pipette 9 to remove the used pipette tips;

[0056] S8, the sample tank 5 after separation is moved by conveyor belt 100 to the immersion and lifting station 201 of the lifting coating machine 200 to start lifting coating.

[0057] Please see Figure 4 When a single lifting coating machine is used for multiple immersion and lifting operations, during the interval between two immersion and lifting operations, the sample tank 5 is transferred back to the sample area 12 of the automated liquid separation device via the conveyor belt 100, and steps S1-S8 are repeated to achieve continuous liquid separation and coating.

[0058] Please see Figure 5 When multiple lifting coating machines are operated simultaneously, the immersion lifting station 201 of each lifting coating machine and the sample area 12 of the automated liquid separation device are connected by the annular conveyor belt 100'. The sample tank 5 is transported to the designated immersion lifting station for lifting coating via the annular conveyor belt. During the interval between two immersion lifting waits, the sample tank 5 is transported to the sample area 12 of the automated liquid separation device using the annular conveyor belt 100'. Steps S1-S8 are repeated to achieve continuous liquid separation and coating.

[0059] The dip coating method of the present invention uses an automatic liquid separation device for liquid separation, so that the height of the inner and outer film solutions in each small compartment of the sample tank 5 is the same. This can maintain the consistency of the outer film height of the sensor after dip coating. Moreover, during the liquid drainage process, the mixing of air is minimized, so that there are no air bubbles on the surface of the outer film after film formation, resulting in good density. In addition, it can reduce the amount of outer film solution used, reduce costs, and improve the efficiency of dip coating.

[0060] In summary, the automated dispensing device and the lifting coating method using the present invention can automatically dispense the outer film solution, effectively reducing the residual amount of the inner and outer film solution on the pipette tip; it can maintain the consistency of the outer film height after lifting and immersion, so that the outer film surface is free of bubbles, forming good density, reducing the amount of outer film solution used, reducing costs, and improving the efficiency of lifting coating.

[0061] Those skilled in the art should recognize that the above embodiments are merely illustrative of the present invention and are not intended to limit the present invention. Any variations or modifications to the above embodiments that are within the spirit and essence of the present invention will fall within the scope of the claims of the present invention.

Claims

1. An automated dispensing device for automatically dispensing outer membrane solutions, characterized in that, It includes a base, a controller, an X-axis robotic arm, and a Y-axis robotic arm, among which: The base is L-shaped, consisting of a horizontal section and a vertical section. The horizontal section of the base is provided with a pipette tip placement area, a sample area, a liquid storage area, a spare area, and a waste pipette tip storage area. The pipette tip placement area is provided with a pipette tip placement slot, the sample area is provided with a sample slot, the liquid storage area is provided with a liquid storage slot, the waste pipette tip storage area is provided with a waste pipette tip storage slot, and the spare area is used to temporarily store the pipette tip placement slot, the sample slot, or the liquid storage slot. An X-axis slide rail is horizontally arranged on the vertical part of the base, and the rear end of the X-axis robotic arm is slidably mounted on the X-axis slide rail and driven by the X-axis drive mechanism. A Y-axis slide rail is longitudinally arranged on one side of the X-axis robotic arm; The Y-axis robotic arm can be slidably mounted on the Y-axis slide rail and driven by the Y-axis drive mechanism; The Y-axis robotic arm has a shell-like structure with an open bottom. Inside the Y-axis robotic arm are a Z-axis slide rail and a multi-channel automatic pipette. The multi-channel automatic pipette can be slidably mounted on the Z-axis slide rail and is driven by a Z-axis lifting cylinder. The controller is installed in the vertical part of the base, and the X-axis drive mechanism, Y-axis drive mechanism, Z-axis lifting cylinder and multi-channel automatic pipette are respectively connected to the controller. The controller is connected to a touch screen. The sample area is connected to the immersion and lifting station of the lifting coating machine by a conveyor belt, which is used for the sample tank to travel back and forth between the sample area and the immersion and lifting station. The multi-channel automatic pipette uses a one-suction-multiple-dispense method for liquid aspiration and dispensing. The multi-channel automatic pipette is detachably mounted on the Z-axis slide rail via a connector.

2. The automated dispensing device for automatically dispensing outer membrane solutions as described in claim 1, characterized in that, The sample area and the pipette tip placement area are located one in front of the other on the left side of the horizontal part of the base, the spare area and the liquid storage area are located one in front of the other in the middle of the horizontal part of the base, and the waste pipette tip storage area is located on the right side of the horizontal part of the base.

3. An automated dispensing device for automatically dispensing outer membrane solutions as described in claim 1, characterized in that, The pipette tip placement slot and the sample slot each have multiple small compartments, and the liquid storage tank has multiple elongated compartments.

4. An automated dispensing device for automatically dispensing outer membrane solutions as described in claim 1, characterized in that, The X-axis drive mechanism and the Y-axis drive mechanism are respectively adopted as stepper motors or cylinders.

5. A method for dip coating, characterized in that, The process of performing a lift coating using a lift coating machine and the automated liquid separation device as described in claim 1 specifically includes the following steps: S1. Prepare multiple pipette tips and place them into the pipette tip storage slots respectively; S2, Prepare the outer membrane solution for lifting and pour it into the storage tank; S3, set the dispensing parameters on the touch screen. The dispensing parameters include the amount of liquid aspirated by the multi-channel automatic pipette and the number of times the liquid is dispensed. Then start the automatic dispensing device. S4, the controller controls the X-axis drive mechanism and the Y-axis drive mechanism to move the X-axis robotic arm and the Y-axis robotic arm in a one-to-one correspondence, so that the Y-axis robotic arm is exactly above the pipette tip placement slot. Then the controller controls the Z-axis lifting cylinder to drive the multi-channel automatic pipette to lower, so that each channel of the multi-channel automatic pipette is connected to a pipette tip. Then the controller controls the Z-axis lifting cylinder to drive the multi-channel automatic pipette to raise. S5, the controller controls the X-axis drive mechanism and the Y-axis drive mechanism to move the X-axis robotic arm and the Y-axis robotic arm in a one-to-one correspondence, so that the Y-axis robotic arm moves to the top of the liquid storage tank. Then the controller controls the Z-axis lifting cylinder to drive the multi-channel automatic pipette to lower. The pipette tip is inserted into the outer membrane solution. The controller controls the multi-channel automatic pipette to aspirate liquid according to the preset dispensing parameters. Each pipette tip aspirates the outer membrane solution at the same time. Then the controller controls the Z-axis lifting cylinder to drive the multi-channel automatic pipette to rise. S6, the controller controls the X-axis drive mechanism and the Y-axis drive mechanism to move the X-axis robotic arm and the Y-axis robotic arm in a one-to-one correspondence, so that the Y-axis robotic arm moves to the sample cell. Then, the controller controls the Z-axis lifting cylinder to drive the multi-channel automatic pipette to lower, so that the bottom of each pipette tip is located in the small compartment of the corresponding sample cell. The controller controls the multi-channel automatic pipette to discharge liquid through multiple sprays according to the preset dispensing parameters, so that the outer membrane solution in the pipette tip is fully and completely injected into the small compartment of the corresponding sample cell. S7. After the drainage action is completed, the controller controls the Z-axis lifting cylinder to lift the multi-channel automatic pipette and controls the X-axis drive mechanism and Y-axis drive mechanism to move the X-axis robotic arm and Y-axis robotic arm in a corresponding manner, so that the Y-axis robotic arm moves above the waste pipette tip storage tank. The controller then controls the multi-channel automatic pipette to remove the used pipette tips. S8, the sample tank after separation is moved by conveyor belt to the immersion and lifting station of the lifting coating machine to start the lifting coating.

6. The dip coating method as described in claim 5, characterized in that, When a single lifting coating machine is used for multiple dip-lift coatings, during the interval between two dip-lift waiting periods, the sample tank is returned to the sample area of ​​the automated liquid separation device via a conveyor belt, and steps S1-S8 are repeated. When multiple lifting coating machines are operated simultaneously, the immersion lifting station of each lifting coating machine and the sample area of ​​the automated liquid separation device are connected by a ring conveyor belt. The sample tank is then transported to the designated immersion lifting station for lifting coating. During the interval between two immersion lifting waits, the sample tank is transported to the sample area of ​​the automated liquid separation device using the ring conveyor belt, and steps S1-S8 are repeated.

7. The dip coating method as described in claim 6, characterized in that, In step S6, after the first spraying is completed, each subsequent spraying is performed by the controller first raising the multi-channel automatic pipette via the Z-axis lifting cylinder, raising the position of the pipette tip above the sample cell, so that the air inside the pipette tip is expelled outwards. Then the controller lowers the multi-channel automatic pipette via the Z-axis lifting cylinder, allowing the outer membrane solution inside the pipette tip to flow into the sample cell.

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