一种冬荪ISSR分子标记引物组、反应体系及其在种群遗传多样性中的应用
By using ISSR molecular marker primer sets and reaction systems for *Fructus medica*, the technical gap in the analysis of genetic diversity of *Fructus medica* populations has been filled, enabling rapid and stable identification of population genetic diversity and analysis of kinship, thus filling the gaps in existing technologies.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- GUIZHOU CROP VARIETIES RESOURCE INST
- Filing Date
- 2022-06-09
- Publication Date
- 2026-07-17
AI Technical Summary
Currently, no methods for using ISSR molecular marker technology for germplasm resource identification and genetic diversity analysis in winter fungus have been reported, and existing technologies are difficult to effectively apply to the population genetic diversity analysis of winter fungus.
We provide ISSR molecular marker primer sets and reaction systems for *Dictyophora indica*, including primer sets with specific nucleotide sequences and optimized PCR reaction conditions. Through ISSR-PCR amplification, electrophoretic separation, and cluster analysis, we construct molecular phylogenetic trees and calculate genetic diversity parameters.
It enables rapid and stable identification of the genetic diversity of *Fructus Epimedii* populations, reveals the kinship among germplasm resources, improves identification efficiency and reliability, and is low in cost and simple to operate.
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Figure CN117247936B_ABST