A lanthanide metal organic framework material, a derived fluorescent sensing test paper, a preparation method and applications

Abstract

The present application relates to a kind of lanthanide metal organic framework material, derivative fluorescent sensing test paper, preparation method and application.Lanthanide metal ion, organic ligand and accelerator are dissolved in ultrapure water, and lanthanide metal organic framework is prepared by hydrothermal reaction, and is preserved after washing, centrifugation.Lanthanide metal organic framework is used as basic reaction unit, and the quantitative detection of the fluorescence enhancement of antibiotic ciprofloxacin is carried out in tris-HCl buffer reagent with pH=9;Lanthanide metal organic framework-ciprofloxacin is used as basic reaction unit, and the quantitative detection of the ratio fluorescence of metal aluminum ion is realized;Lanthanide metal organic framework material is loaded on filter paper, and portable fluorescent detection test paper is constructed.Compared with prior art, the preparation method of the present application is simple, low in cost, and has lower detection limit for ciprofloxacin, and has excellent selectivity.In addition, the detection test paper constructed in the present application improves the easy operability, portability and flexibility of detection.

Description

Technical Field

[0001] This invention relates to the fields of metal-organic framework material preparation and fluorescence detection technology, and in particular to a lanthanide metal-organic framework material, a derived fluorescence sensing paper, a preparation method, and applications. Background Technology

[0002] Ciprofloxacin, as the most commonly used fluoroquinolone antibiotic, plays an important role in the treatment of bacterial infections in animals and humans due to its advantages such as good antibacterial activity, low toxicity, and low cost. However, ciprofloxacin is slowly metabolized and degraded, is not easily absorbed, and large-scale discharge can lead to serious water pollution. Ciprofloxacin can be transmitted through the food chain and eventually accumulate in the human body, harming human health. Many countries and organizations have established maximum residue limits for ciprofloxacin in water and certain foods. Therefore, the detection of ciprofloxacin residues in the environment is of vital importance for environmental protection, pollution control, and the protection of human health. Aluminum (Al) is the third most abundant metal ion in the Earth's crust and has wide applications. However, Al... 3+ The toxicity of Al has also raised concerns. The World Health Organization stipulates that Al in drinking water... 3+ The maximum permissible concentration is 7.41 μM.

[0003] Ciprofloxacin and Al 3+ There are various qualitative and quantitative analysis methods for target analytes of environmental pollutants. Among them, traditional laboratory instrument methods, mainly high-performance liquid chromatography (HPLC), mass spectrometry (MS), and enzyme-linked immunosorbent assay (ELISA), are the most commonly used. However, these techniques rely on expensive instruments, complex analytical techniques and sample pretreatment, and require highly skilled operators, making them unsuitable for on-site analysis and detection. Therefore, there is an urgent need to explore a rapid, simple, and portable on-site detection method for the portable detection of environmental pollutants. Fluorescence sensing technology is a rapidly developing environmental detection technology in recent years, and its advantages such as rapid response, simplicity, portability, and adaptability have made it one of the most ideal methods.

[0004] Various fluorescent probes have been developed, including traditional fluorescent probes such as organic fluorescent dyes, semiconductor quantum dots, and metal nanoclusters, as well as some emerging fluorescent materials such as carbon quantum dots and fluorescent carbon nanotubes. However, these fluorescent probes generally suffer from various drawbacks. For example, organic fluorescent dyes have poor stability and narrow excitation spectra; semiconductor quantum dots have a certain degree of toxicity; metal nanoclusters are costly; and carbon quantum dots have complex compositions and low fluorescence yield. Furthermore, most fluorescent probes can only identify one substance at a time, making it difficult to achieve simultaneous detection of two pollutants with a single probe—a common problem with current fluorescent probes.

[0005] Therefore, it is necessary to establish a simple, accurate method for the simultaneous quantitative detection of ciprofloxacin and Al in food and the environment. 3+ This approach represents a promising research direction. Summary of the Invention

[0006] The purpose of this invention is to overcome the shortcomings of the existing technology by providing a lanthanide metal-organic framework material, a derived fluorescent sensing paper, a preparation method, and applications. This invention can detect ciprofloxacin and Al... 3+ It enables cascaded quantitative detection with excellent selectivity. Furthermore, by constructing portable fluorescent test strips, the detection method is simple, easy to scale up, and inexpensive, making it suitable for on-site testing.

[0007] The objective of this invention can be achieved through the following technical solutions:

[0008] This invention provides a method for preparing lanthanide metal-organic framework materials. The method includes: dissolving lanthanide metal ions, organic ligands and promoters in ultrapure water and sonicating them, then preparing lanthanide metal-organic framework materials through hydrothermal reaction, and then washing, centrifuging, purifying and vacuum drying the prepared lanthanide metal-organic framework materials for storage.

[0009] Furthermore, the lanthanide metal ion is europium ion, the organic ligand is isophthalic acid, and the promoter is 2-methylimidazole;

[0010] The molar ratio of europium ions, isophthalic acid, and dimethylimidazole is 1:0.5:1.

[0011] Furthermore, the ultrasonic time is 15 minutes; the washing uses anhydrous ethanol and deionized water, and the washing sequence is alternating between anhydrous ethanol and deionized water twice; the centrifugation speed is set to 8000 r / min and the centrifugation time is 10 minutes; the vacuum drying temperature is 60°C.

[0012] The present invention also provides a lanthanide metal-organic framework material, characterized in that the lanthanide metal-organic framework material has a rod-like morphology.

[0013] This invention also provides an application of lanthanide metal-organic framework materials for the detection of ciprofloxacin and Al. 3+ The detection.

[0014] Furthermore, the specific method for detecting ciprofloxacin is as follows: a lanthanide metal-organic framework material is dispersed in ultrapure water to obtain a dispersion, and Tris-HCl buffer, lanthanide metal-organic framework material dispersion, and ciprofloxacin solutions of different concentrations are added to the detection bottle, with a volume ratio of (0.4-3):(0.4-2):2.

[0015] For Al 3+ The specific detection method is as follows: Lanthanide metal-organic framework-ciprofloxacin reaction unit and different concentrations of Al are added to the detection bottle respectively. 3+ The solution has a volume ratio of 4:1.

[0016] Furthermore, the lanthanide metal-organic framework-ciprofloxacin reaction unit is a mixture of Tris-HCl buffer, lanthanide metal-organic framework dispersion and ciprofloxacin solutions of different concentrations, in a volume ratio of 1:1:2.

[0017] The present invention also provides a fluorescent sensing test strip derived from a lanthanide metal-organic framework material, the fluorescent sensing test strip comprising a filter paper and a lanthanide metal-organic framework material, wherein the lanthanide metal-organic framework material is loaded on the filter paper.

[0018] The present invention also provides a method for preparing a fluorescent sensing test paper derived from lanthanide metal-organic framework materials. The preparation method includes: ultrasonically dispersing lanthanide metal-organic framework materials in ultrapure water, immersing filter paper cut into square pieces in the ultrapure water and oscillating for 12-36 hours, and then taking it out and drying it to obtain a fluorescent sensing test paper loaded with lanthanide metal-organic framework materials.

[0019] This invention also provides an application of a fluorescent sensing strip derived from a lanthanide metal-organic framework material, wherein the fluorescent sensing strip is used for the detection of ciprofloxacin and Al 3+ Fluorescence detection;

[0020] The specific method for detecting ciprofloxacin fluorescence is as follows: immerse the fluorescent sensor paper in ciprofloxacin solutions of different concentrations, remove it after soaking for a period of time, dry it, and then perform fluorescence detection.

[0021] For Al 3+ The specific fluorescence detection method is as follows: a certain amount of Al at different concentrations is added to test strips that have been tested for the same concentration of ciprofloxacin. 3+ And perform fluorescence detection.

[0022] Compared with the prior art, the present invention has the following advantages and beneficial effects:

[0023] 1. The lanthanide metal-organic framework material prepared by this invention has a metal center that can specifically recognize ciprofloxacin with strong selectivity; the porous structure can achieve the enrichment of ciprofloxacin and reduce its detection limit; the preparation steps are simple, pollution-free, and the composition is adjustable.

[0024] 2. In this invention, lanthanide metal-organic framework materials are loaded onto filter paper to fix the powder material, thereby enhancing the recyclability of the sensing material. By combining the lanthanide metal-organic framework materials with test paper, a portable test paper is constructed, which can be used for on-site testing, thus improving its applicability.

[0025] 3. The portable fluorescent test strip prepared by this invention can sequentially detect pollutants in two environments, greatly improving detection efficiency and utilization efficiency of fluorescent materials. Attached Figure Description

[0026] Figure 1 This is a flowchart of a method for preparing a lanthanide metal-organic framework material in Example 1;

[0027] Figure 2 This is a scanning electron microscope image of a lanthanide metal-organic framework material from Example 1;

[0028] Figure 3 This is a diagram illustrating the preparation method and detection sequence of a fluorescent sensing test strip derived from a lanthanide metal-organic framework material, as shown in Example 1.

[0029] Figure 4 The fluorescent sensing strip derived from the lanthanide metal-organic framework material in Example 1 was used to detect ciprofloxacin and Al. 3+ Optical images;

[0030] Figure 5 The fluorescence detection spectrum of ciprofloxacin by the lanthanide metal-organic framework material in Example 4 is shown.

[0031] Figure 6 The lanthanide metal-organic framework material in Example 4 is used to support Al 3+ Fluorescence detection spectrum;

[0032] Figure 7 The graph shows the selective detection results of ciprofloxacin by the lanthanide metal-organic framework material in Comparative Example 1.

[0033] Figure 8 The figure shows the selective detection results of ciprofloxacin by the lanthanide metal-organic framework material in Comparative Example 2. Detailed Implementation

[0034] The following examples illustrate specific implementations of the present invention. These examples are carried out based on the solution described in the present invention, and provide detailed implementation methods and specific operating procedures. However, the scope of protection of the present invention is not limited to the following examples.

[0035] The present invention will be further described below with reference to the accompanying drawings and specific embodiments. Component models, material names, connection structures, and other features not explicitly stated in this technical solution are considered common technical features disclosed in the prior art. The materials and reagents used in the embodiments and comparative examples were all purchased commercially.

[0036] A method for preparing a lanthanide metal-organic framework material, the method specifically comprising: using lanthanide metal ions and organic ligands as the basic reaction system, adding a promoter, and dissolving them together in ultrapure water under ultrasonic conditions; transferring the mixed solution into a reactor and carrying out a hydrothermal reaction under high temperature conditions; after the reaction is completed, washing, centrifuging, purifying and storing the prepared lanthanide metal-organic framework material.

[0037] An application of a lanthanide metal-organic framework material for the quantitative detection of ciprofloxacin and al in water. 3+ The specific steps for fluorescence detection of ciprofloxacin are as follows: Prepare gradient concentrations of ciprofloxacin. A 1 mM ciprofloxacin solution is prepared as a stock solution and then serially diluted. The prepared lanthanide metal-organic framework material is dispersed in ultrapure water to obtain a lanthanide metal-organic framework dispersion. Tris-HCl buffer solution (pH=9), the lanthanide metal-organic framework dispersion, and gradient concentrations of ciprofloxacin solution are then added to the detection vial, and the fluorescence intensity is measured using a fluorescence spectrophotometer.

[0038] Fluorescence detection Al 3+ The specific steps are as follows: Prepare the fluorescent precursor probe by adding Tris-HCl buffer (pH=9), lanthanide metal-organic framework dispersion, and ciprofloxacin solutions of different concentrations to the detection vial in a volume ratio of 1:1:2. Prepare gradient concentrations of Al... 3+ A 20 mM anhydrous aluminum chloride (AlCl3) solution was prepared as a stock solution and then serially diluted. Lanthanide metal-organic framework-ciprofloxacin fluorescent precursor probe and gradient concentrations of AlCl3 were added to the detection vials, respectively. 3+ The fluorescence intensity of the solution was measured using a fluorescence spectrophotometer.

[0039] An application of a fluorescent sensing test strip derived from a lanthanide metal-organic framework material was described, enabling the construction of a portable detection device based on the test strip for the detection of ciprofloxacin and Al. 3+ Conduct portable testing.

[0040] The specific preparation method of the portable detection device is as follows: Lanthanide metal-organic framework materials are ultrasonically dispersed in ultrapure water, and filter paper cut into squares is immersed in it. After shaking and incubating for a period of time, the filter paper is taken out, dried and ready for use to obtain portable test paper, which is then used as a fluorescent sensing test paper for further detection.

[0041] The specific method for using the portable detection device is as follows: Immerse the portable fluorescent test strip in ciprofloxacin solutions of different concentrations for 3 seconds, then remove and dry it, and measure its fluorescence; add 10 μL of Al solutions of different concentrations to the test strips that have been tested for the same concentration of ciprofloxacin. 3+ And its fluorescence was measured.

[0042] Example 1

[0043] This embodiment provides a method for preparing lanthanide metal-organic framework materials, such as... Figure 1 As shown, 0.366 g of EuCl3·6H2O, 0.083 g of isophthalic acid, and 0.082 g of 2-methylimidazole were dissolved in 10 mL of ultrapure water and sonicated for 15 min to promote dissolution. The above mixed solution was transferred to a 50 mL polytetrafluoroethylene-lined reactor and reacted at 150 °C for 72 h to obtain a powder. The obtained powder was repeatedly washed with anhydrous ethanol and ultrapure water, with the washing sequence being alternating between anhydrous ethanol and deionized water twice. After centrifugation at 8000 rpm for 10 min, it was finally vacuum dried overnight at 60 °C to obtain a white powder, namely the lanthanide metal-organic framework material Eu MOFs.

[0044] This embodiment also provides a lanthanide metal-organic framework material, such as Figure 2 As shown. Figure 2 The image shows a scanning electron microscope (SEM) image of the prepared Eu MOFs. As can be seen from the image, the prepared lanthanide metal-organic framework material is rod-shaped, indicating that a lanthanide metal-organic framework material with a rod-shaped structure has been successfully prepared.

[0045] This embodiment also provides a fluorescent sensing test strip derived from a lanthanide metal-organic framework material. The fluorescent sensing test strip includes a filter paper and a lanthanide metal-organic framework material, with the lanthanide metal-organic framework material loaded on the filter paper.

[0046] This embodiment also provides a method for preparing fluorescent sensing test strips derived from lanthanide metal-organic framework materials, such as... Figure 3 As shown, 20 mg of the prepared Eu MOFs were ultrasonically dispersed in 20 mL of ultrapure water, and the rapid qualitative filter paper was cut into 1 cm pieces. 2 The paper strips were immersed in the above Eu MOFs dispersion, shaken and cultured at room temperature for 24 h, and then dried at 60 °C to obtain fluorescent sensing paper derived from lanthanide metal-organic framework materials.

[0047] This embodiment also provides an application of a fluorescent sensing test strip derived from a lanthanide metal-organic framework material, wherein the fluorescent sensing test strip is used for the detection of ciprofloxacin and Al. 3+ The fluorescence detection method is as follows:

[0048] (1) Paper-based fluorescence detection of ciprofloxacin: A 1 mM ciprofloxacin solution was prepared as a stock solution and then serially diluted to prepare ciprofloxacin solutions with concentrations of 300, 325, 350, 375, 400, 425, 450, 475, 500, and 550 μM. A fluorescent test strip loaded with Eu MOFs was picked up with tweezers and immersed in the ciprofloxacin solution for 3 seconds. It was then removed and allowed to air dry. The test strip was then placed under a 365 nm UV lamp and photographed with a smartphone; the image was easily identifiable by the human eye.

[0049] (2)Al 3+ Paper-based fluorescence detection: A batch of fluorescent test papers loaded with Eu MOFs were pre-immersed in a 500 μM ciprofloxacin solution for 3 seconds, then removed and allowed to air dry naturally, serving as the detection material for Al. 3+ Fluorescent test strip No. 2. A 20 mM anhydrous aluminum chloride (AlCl3) solution was prepared as a stock solution and then serially diluted to prepare AlCl3 solutions with concentrations of 0, 1, 3, 5, 6, 8, 10, 11, 13, 15, 16, 18, and 20 mM. 3+ Solution. Add 10 μL of Al at different concentrations to the above fluorescent test paper No. 2. 3+ Allow it to air dry naturally. Then place the test strip under a 365nm wavelength ultraviolet light and photograph it with a smartphone; the result is easily identifiable by the human eye.

[0050] Figure 4 The fluorescent sensing strip derived from the lanthanide metal-organic framework material prepared in Example 1 is used to detect ciprofloxacin and Al. 3+ Optical images of fluorescence detection show that, as the concentration of ciprofloxacin increases, the fluorescent sensing strip gradually changes from no fluorescence to a bright red fluorescence; while with increasing Al... 3+ As the concentration increases, the fluorescent sensing strip gradually changes from bright red fluorescence to bright blue fluorescence. Therefore, this invention achieves visual detection, with intuitive and visible results, and a simple and portable detection process, greatly improving the portability of the detection.

[0051] Example 2

[0052] The difference between this embodiment and Example 1 is that in the preparation method of a fluorescent sensing test paper derived from a lanthanide metal-organic framework material, the cut filter paper is immersed in Eu MOF dispersion and cultured with shaking for 12 hours. The rest of the preparation method is the same as in Example 1.

[0053] Example 3

[0054] The difference between this embodiment and Example 1 is that in the preparation method of a fluorescent sensing test paper derived from a lanthanide metal-organic framework material, the cut filter paper is immersed in Eu MOF dispersion and cultured with shaking for 36 hours. The rest of the preparation method is the same as in Example 1.

[0055] Example 4

[0056] This embodiment applies the lanthanide metal-organic framework material prepared in Example 1 to the treatment of ciprofloxacin and Al 3+ The specific steps for fluorescence detection are as follows:

[0057] (1) Fluorescence detection of ciprofloxacin: A 1 mM ciprofloxacin solution was prepared as a stock solution and then serially diluted to prepare ciprofloxacin solutions with concentrations of 0, 40, 80, 120, 140, 160, 180, 220, 240, 260, 280, 300, 320, 360, 400, 440, and 480 μM. Eu MOF dispersions were then prepared by dispersing 5 mg of Eu MOF powder in 20 mL of ultrapure water and sonicating for 1 min before use to ensure uniform dispersion. 0.5 mL of Tris-HCl buffer solution (pH = 9), 0.5 mL of Eu MOF dispersion, and 1 mL of ciprofloxacin solutions of varying concentrations were added sequentially to the detection bottle. After sufficient reaction, the reaction solution was obtained. The fluorescence spectrum of the reaction solution at an excitation wavelength of 365 nm was detected using a fluorescence spectrophotometer.

[0058] (2)Al 3+ Fluorescence detection: A 20 mM anhydrous aluminum chloride (AlCl3) solution was prepared as a stock solution and then serially diluted to prepare AlCl3 solutions with concentrations of 0, 1, 3, 5, 6, 8, 10, 11, 13, 15, 16, 18 and 20 mM. 3+ Solution. Prepare the Eu MOFs-ciprofloxacin fluorescent precursor probe by adding 5 mL of pH 9 Tris-HCl buffer, 5 mL of EuMOFs dispersion, and 10 mL of 500 μM ciprofloxacin solution to the stock vial. Then, add 0.8 mL of the Eu MOFs-ciprofloxacin fluorescent precursor probe and 0.2 mL of a gradient concentration of Al2O3 to the detection vial. 3+ The solution was allowed to react completely to obtain the reaction solution to be tested. The fluorescence spectrum of the reaction solution at an excitation wavelength of 365 nm was measured using a fluorescence spectrophotometer.

[0059] Figure 5 To obtain the fluorescence detection spectrum of ciprofloxacin using the lanthanide metal-organic framework material prepared in Example 1, by... Figure 5It is known that ciprofloxacin can significantly enhance the fluorescence intensity of Eu MOFs at the emission wavelength of 616 nm, and the fluorescence intensity also increases as the concentration of ciprofloxacin increases between 0 and 240 μM. Figure 6 To use the lanthanide metal-organic framework material prepared in Example 1 for Al 3+ The fluorescence detection spectrum, by Figure 6 It can be seen that adding Al to the Eu MOFs-ciprofloxacin system 3 + At that time, the fluorescence of the solution at the emission wavelength of 616 nm was quenched, while the fluorescence at the emission wavelength of 425 nm was enhanced.

[0060] Example 5

[0061] The difference between this embodiment and Embodiment 4 is that, in the fluorescence detection of ciprofloxacin, 0.2 mL of Tris-HCl buffer solution with pH=9 is added to the detection bottle; the rest of the contents are the same as in Embodiment 4.

[0062] Example 6

[0063] The difference between this embodiment and Embodiment 4 is that, in the fluorescence detection of ciprofloxacin, 1 mL of Tris-HCl buffer solution with pH=9 is added to the detection bottle; the rest of the contents are the same as in Embodiment 4.

[0064] Example 7

[0065] The difference between this embodiment and Embodiment 4 is that, in the fluorescence detection of ciprofloxacin, 0.2 mL of Eu MOF dispersion is added to the detection bottle; the rest of the contents are the same as in Embodiment 4.

[0066] Example 8

[0067] The difference between this embodiment and Embodiment 4 is that, in the fluorescence detection of ciprofloxacin, 0.7 mL of Eu MOF dispersion was added to the detection bottle; the rest of the contents are the same as in Embodiment 4.

[0068] Comparative Example 1

[0069] The difference between this comparative example and Example 4 is that, in the fluorescence detection of ciprofloxacin, the 1 mL gradient ciprofloxacin solution was replaced with 500 μM tetracycline solution, chloramphenicol solution, norfloxacin solution, kanamycin sulfate solution, glycine solution, glutamic acid solution, L-cysteine ​​solution, L-ascorbic acid solution, glutathione solution, ciprofloxacin solution, and blank solution. The rest of the contents are the same as in Example 4. Figure 7 This is a graph showing the selective detection results of ciprofloxacin using the lanthanide metal-organic framework material prepared in Example 1. From... Figure 7The results show that this lanthanide metal-organic framework material exhibits good selectivity for the fluorescence detection of ciprofloxacin in a variety of similar organic compounds.

[0070] Comparative Example 2

[0071] The difference between this comparative example and Example 4 is that, in the case of Al... 3+ In the fluorescence detection, 0.2 mL of Al at gradient concentrations will be added. 3+ The solution was replaced with Ni solution at a concentration of 20 mM. 2+ Solution Ba 2+ , solution Cd 2+ , solution, K + Solution, Zn 2+ Solution, Na + Solution, Ca 2+ Solution, Pb 2+ Solution, Cu 2+ Solution, Al 3+ The solution and blank solution are the same as in Example 4.

[0072] Figure 8 To use the lanthanide metal-organic framework material prepared in Example 1 for Al 3+ The selective detection results are shown in the image. Figure 8 The results show that this lanthanide metal-organic framework material exhibits good performance in the presence of Al ions among various metal ions. 3+ The fluorescence detection exhibits good selectivity.

[0073] The above description of the embodiments is provided to enable those skilled in the art to understand and use the invention. It will be apparent to those skilled in the art that various modifications can be made to these embodiments, and the general principles described herein can be applied to other embodiments without inventive effort. Therefore, the present invention is not limited to the above embodiments, and any improvements and modifications made by those skilled in the art based on the disclosure of the present invention without departing from the scope of the invention should be within the protection scope of the present invention.

Claims

1. A lanthanide metal-organic framework material for the detection of ciprofloxacin and The application of [the technology] is characterized by, The lanthanide metal-organic framework material was prepared by the following method: lanthanide metal ions, organic ligands, and a promoter were dissolved in ultrapure water and ultrasonically treated, followed by hydrothermal reaction to obtain the lanthanide metal-organic framework material. The obtained lanthanide metal-organic framework material was washed, centrifuged, purified, and vacuum dried before storage. The lanthanide metal ion was europium ion, the organic ligand was isophthalic acid, and the promoter was 2-methylimidazole, with a molar ratio of europium ion, isophthalic acid, and 2-methylimidazole of 1:0.5:

1.

2. The application according to claim 1, characterized in that, In the preparation method, the ultrasonic time is 15 min; the washing uses anhydrous ethanol and deionized water, and the washing sequence is alternating between anhydrous ethanol and deionized water twice; the centrifugation speed is set to 8000 r / min and the centrifugation time is 10 min; the vacuum drying temperature is 60 ℃.

3. The application according to claim 1 or 2, characterized in that, The specific method for detecting ciprofloxacin is as follows: Lanthanide metal-organic framework materials are dispersed in ultrapure water to obtain a dispersion. Tris-HCl buffer, the lanthanide metal-organic framework material dispersion, and ciprofloxacin solutions of different concentrations are added to the detection bottle in a volume ratio of (0.4-3):(0.4-2):

2. The specific detection method is as follows: Lanthanide metal-organic framework-ciprofloxacin reaction unit and different concentrations of [unspecified substance] are added to the detection bottle respectively. The solution has a volume ratio of 4:

1.

4. The application according to claim 3, characterized in that, The lanthanide metal-organic framework-ciprofloxacin reaction unit consists of a Tris-HCl buffer solution, a lanthanide metal-organic framework dispersion, and ciprofloxacin solutions of different concentrations, mixed in a volume ratio of 1:1:

2.

5. A fluorescent sensing test strip derived from a lanthanide metal-organic framework material, characterized in that, The fluorescent sensing strip includes a filter paper and a lanthanide metal-organic framework material loaded on the filter paper, wherein the lanthanide metal-organic framework material... Prepared according to the method described in claim 1 or 2.

6. The method for preparing the fluorescent sensing test strip according to claim 5, characterized in that, The preparation method includes: ultrasonically dispersing lanthanide metal-organic framework materials in ultrapure water, immersing square filter paper pieces in the ultrapure water and oscillating for 12-36 h, and then taking them out and drying them to obtain fluorescent sensing paper loaded with lanthanide metal-organic framework materials.

7. The application of the fluorescent sensing test strip according to claim 5, characterized in that, The fluorescent sensing strip is used for the detection of ciprofloxacin and... Fluorescence detection; the specific method for ciprofloxacin fluorescence detection is as follows: fluorescent sensor paper is immersed in ciprofloxacin solutions of different concentrations, soaked for a period of time, then removed, dried, and subjected to fluorescence detection; The specific fluorescence detection method is as follows: A certain amount of different concentrations of ciprofloxacin is added to test strips that have previously tested for the same concentration of ciprofloxacin. And perform fluorescence detection.

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