Monoclonal antibody of chicken parvovirus and application thereof

By preparing monoclonal antibodies 1B12 and 2B2 against the NS1 protein of chicken parvovirus, a double-antibody sandwich ELISA detection platform was established, which solved the problem of the lack of specific detection tools for ChPV virus in the existing technology, and realized antigen detection with high sensitivity and specificity, supporting rapid detection and prevention in the poultry industry.

CN117285619BActive Publication Date: 2025-11-25GUANGXI VETERINARY RES INST
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Patent Information

Application Number
CN202311271713.7
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2023-09-28
Publication Date
2025-11-25
Estimated Expiration
2043-09-28

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Abstract

The application provides a chicken parvovirus monoclonal antibody and application thereof, relates to the field of biotechnology and virology, and 2 chicken parvovirus NS1 protein monoclonal antibodies screened by the application can specifically combine with chicken parvovirus and in-vitro expressed NS1 protein, and the light chains of the 2 monoclonal antibodies are kappa chains, and the heavy chains are IgG1. A double antibody sandwich ELISA detection platform of ChPV NS1 protein is established by taking monoclonal antibody 1B12 as a coating antibody, the minimum detection line of the chicken parvovirus reaches 31.2 ng / mL, the specificity is strong, the sensitivity is high, the stability is good, and parallel detection of 192 clinical samples by the double antibody sandwich ELISA detection platform and nested PCR shows that the total coincidence rate reaches 89.1%. The chicken parvovirus monoclonal antibody provided by the application can be used for preparing chicken parvovirus related detection reagents, and also provides raw materials for basic researches on functions of the chicken parvovirus NS1 protein, is helpful for prevention and control of the chicken parvovirus in the poultry industry, and is also helpful for basic researches on the chicken parvovirus related proteins.
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