An apparatus and method for evaluating anaerobic ammonia oxidation bacteria

Abstract

The application provides an anaerobic ammonia oxidation bacteria evaluation device and an evaluation method. The evaluation device is simple in structure, and can be used for real-time monitoring of related parameters of an anaerobic reaction and gas production phenomena based on the characteristics of an anaerobic ammonia oxidation reaction. The evaluation method for evaluating a sample to be evaluated by using the evaluation device can be used for evaluating whether the sample to be evaluated contains anaerobic ammonia oxidation bacteria and / or evaluating the activity of the bacteria in the sample containing anaerobic ammonia oxidation bacteria. The evaluation method is simple in operation and accurate in result. The anaerobic ammonia oxidation bacteria evaluation device and the evaluation method are applied to the evaluation of anaerobic ammonia oxidation sludge, and whether the anaerobic ammonia oxidation sludge can be used for an anaerobic ammonia oxidation reaction or the activity of anaerobic ammonia oxidation bacteria in the sludge can be effectively evaluated, so that the anaerobic ammonia oxidation sludge can be quickly and accurately determined, and the application prospect of the anaerobic ammonia oxidation sludge in the field of wastewater anaerobic ammonia oxidation denitrification is very good.

Description

Technical Field

[0001] This invention belongs to the field of wastewater treatment and bacterial evaluation technology, specifically an anaerobic ammonia-oxidizing bacteria evaluation device and evaluation method. Background Technology

[0002] There are physical, chemical, and biological methods for removing nitrogen pollutants from water. Compared with physical and chemical methods, biological methods are less expensive, easier to operate, and do not produce secondary pollution. Existing biological denitrification processes mainly include nitrification-denitrification (SND), short-cut nitrification (SHARON), anammox, or nitrite-based nitrification-anammox (SHARON-ANAMMOX) technology.

[0003] The principle of the SND process is that, under aerobic conditions, nitrifying bacteria convert NH4+ into nitrogen dioxide. + -N is first converted to NO2 - -N is then converted to NO3 - -N, and then under anaerobic conditions, denitrifying bacteria will produce NO3. - -N is first converted to NO2 - -N is then converted to N2. The principle of the SHARON process is that, under aerobic conditions, autotrophic nitrite-oxidizing bacteria convert NH4+ into N2. + -N is converted to NO2 - -N, and then under anaerobic conditions, heterotrophic denitrifying bacteria use organic matter as an electron donor and NO2 as the base. - As an electron acceptor, NO2 - -N is converted to N2. Both the SND and SHARON processes require oxygen and organic carbon sources for nitrogen removal. The ANAMMOX process operates on the principle that under anaerobic conditions, microorganisms use NH4+ to... + NO2 acts as an electron donor. - As an electron acceptor, NH4 + -N, NO2 - -N is converted to N2. The ANAMMOX process has the advantages of high efficiency, low operating cost and low sludge production when used to treat wastewater containing high ammonia nitrogen.

[0004] With in-depth research by domestic and international scientists on the ANAMMOX process, relevant literature reports on its mechanisms and processes have been published, and it has been gradually applied to wastewater treatment, achieving good economic and ecological benefits. Anaerobic ammonia oxidation sludge is crucial for anaerobic ammonia oxidation (ANAO) denitrification. If the wrong sludge is used in the initial startup phase, or if the bacterial activity of the ANAO sludge is low, the ANAO denitrification process will be severely affected. Currently, there are few reports on evaluation systems or methods for ANAO sludge. Summary of the Invention

[0005] To address the shortcomings of existing technologies, the present invention aims to provide an evaluation device and method for anaerobic ammonia-oxidizing bacteria. The evaluation device of the present invention has a simple structure, the evaluation method is easy to operate, and the results are accurate. Applying the evaluation device and method of the present invention to the evaluation of anaerobic ammonia-oxidizing sludge can quickly and accurately determine whether the anaerobic ammonia-oxidizing sludge can be used for anaerobic ammonia oxidation reactions, or effectively assess the activity of anaerobic ammonia-oxidizing bacteria in the sludge, showing excellent application prospects in the field of anaerobic ammonia oxidation denitrification of wastewater.

[0006] The objective of this invention is achieved through the following technical solution:

[0007] In a first aspect, the present invention provides an anaerobic ammonia-oxidizing bacteria evaluation device, the evaluation device comprising an anaerobic reactor, a gas-liquid separator, a CO2 generator, and a gas bag.

[0008] According to the anaerobic ammonia-oxidizing bacteria evaluation device provided by the present invention, the top of the gas-liquid separator is provided with an outlet and an inlet, the outlet being connected to a gas bag and the inlet being connected to a CO2 generator. Preferably, the inlet and outlet of the gas-liquid separator are the same opening.

[0009] According to the anaerobic ammonia-oxidizing bacteria evaluation device provided by the present invention, the anaerobic reactor is provided with a circulating water outlet at the top and a circulating water inlet at the bottom; both the circulating water outlet and the circulating water inlet are connected to a gas-liquid separator to realize water circulation between the anaerobic reactor and the gas-liquid separator. Preferably, the gas-liquid separator is connected to the circulating water inlet of the anaerobic reactor through its outlet and to the circulating water outlet of the anaerobic reactor through its inlet, and the outlet of the gas-liquid separator is lower than the inlet.

[0010] In some embodiments of the present invention, a circulating pump is further provided between the gas-liquid separator and the circulating water inlet. The inlet of the circulating pump is connected to the outlet of the gas-liquid separator, and the outlet of the circulating pump is connected to the circulating water inlet.

[0011] Preferably, the circulating pump is a booster pump, so as to pressurize the water coming out of the gas-liquid separator and then send it into the anaerobic reactor through the circulating water inlet.

[0012] In some embodiments of the present invention, a pH meter is also provided between the circulating water inlet and the gas-liquid separator to monitor the pH value of the water; the gas outlet of the gas-liquid separator is also connected to a water seal bottle.

[0013] Preferably, the gas outlet pipe on the gas-liquid separator is a three-way pipe, with one end connected to the gas outlet of the gas-liquid separator, one end connected to the gas bag, and the other end connected to the water seal bottle.

[0014] In some embodiments of the present invention, a sampling port is also provided on the anaerobic reactor for sampling and analyzing water samples inside the anaerobic reactor. Preferably, the sampling port is located at the lower part of one side wall of the anaerobic reactor and can also serve as a drain outlet for emptying the liquid inside the anaerobic reactor.

[0015] In some embodiments of the present invention, the device further includes a nitrogen source, and a nitrogen inlet connected to the nitrogen source is provided at the bottom of the anaerobic reactor; preferably, the air inlet of the gas-liquid separator is also connected to the nitrogen source. More preferably, the air inlet pipe on the gas-liquid separator is a three-way pipe, one end of which is connected to the air inlet of the gas-liquid separator, one end of which is connected to the CO2 generator, and the other end of which is connected to the nitrogen source.

[0016] In some embodiments of the present invention, a dissolved oxygen meter is further installed between the circulating water inlet and the gas-liquid separator to monitor the dissolved oxygen content of the water. Preferably, the dissolved oxygen meter can monitor in real time online.

[0017] More preferably, the circulating pump is located near the circulating water inlet, and the dissolved oxygen meter and pH meter are located near the outlet of the gas-liquid separator.

[0018] According to the anaerobic ammonia-oxidizing bacteria evaluation device provided by the present invention, the evaluation device further includes a feeding tank, and an inlet connected to the feeding tank is provided on the anaerobic reactor. Preferably, the inlet is provided on the side wall of the anaerobic reactor. More preferably, a feeding pump is provided at the outlet of the feeding tank. The inlet of the feeding pump is connected to the outlet of the feeding tank, and the outlet of the feeding pump is connected to the inlet, so as to facilitate the feeding of nitrogen-containing wastewater from the inlet of the anaerobic reactor into the anaerobic reactor for denitrification treatment.

[0019] According to the anaerobic ammonia-oxidizing bacteria evaluation device provided by the present invention, the device further includes a temperature control system, which comprises a jacket disposed outside the anaerobic reactor and a heating tank connected thereto. The temperature control system controls the water temperature inside the anaerobic reactor so that the denitrification reaction occurring inside the anaerobic reactor can proceed within a set temperature range, ensuring the smooth progress of the denitrification reaction while reducing the influence of ambient temperature on the evaluation results, thereby improving the accuracy of the evaluation.

[0020] Preferably, the jacket is installed in the straight section of the anaerobic reactor, and the jacket is circulatedly connected to the heating tank. Specifically, the liquid outlet of the jacket is connected to the liquid inlet of the heating tank, and the liquid inlet of the jacket is connected to the liquid outlet of the heating tank. A heating pump is also installed between the liquid inlet of the jacket and the liquid outlet of the heating tank. The liquid heated by the heating tank is pumped into the jacket by the heating pump. After heat exchange with the anaerobic reactor within the jacket, the liquid returns to the heating tank from the liquid outlet of the jacket. The flow direction of the liquid within the jacket can be the same as or opposite to the flow direction of the wastewater within the anaerobic reactor.

[0021] In some embodiments of the present invention, the anaerobic reactor is an upflow reactor with a height-to-diameter ratio of 7 to 20. Preferably, the anaerobic reactor may be made of glass, plexiglass, or other materials. More preferably, the volume of the anaerobic reactor is 500 to 1000 mL.

[0022] In some embodiments of the present invention, the CO2 generator may be an apparatus that generates CO2 by neutralization reaction of NaHCO3 and HCl.

[0023] Secondly, the present invention provides a method for evaluating anaerobic ammonia-oxidizing bacteria, which uses the anaerobic ammonia-oxidizing bacteria evaluation device described in the first aspect to evaluate the sample to be evaluated.

[0024] According to the anaerobic ammonia-oxidizing bacteria evaluation method provided by the present invention, the method includes evaluating whether the sample to be evaluated contains anaerobic ammonia-oxidizing bacteria and / or evaluating the activity of bacteria in the sample containing anaerobic ammonia-oxidizing bacteria.

[0025] In some embodiments of the present invention, the method for evaluating whether a sample to be evaluated contains anaerobic ammonia-oxidizing bacteria includes placing the sample to be evaluated in an anaerobic reactor, introducing wastewater containing nitrogen pollutants, circulating the wastewater between the anaerobic reactor and a gas-liquid separator for a period of time, and determining whether the sample to be evaluated contains anaerobic ammonia-oxidizing bacteria by observing the pH value changes monitored by a pH meter and the venting situation in the water seal bottle; if gas is continuously generated in the water seal bottle and the pH value of the water body is increased as monitored by the pH meter, it indicates that the sample to be evaluated contains anaerobic ammonia-oxidizing bacteria.

[0026] Preferably, when the wastewater circulates between the anaerobic reactor and the gas-liquid separator, nitrogen gas is intermittently introduced into the anaerobic reactor and / or the gas-liquid separator to remove dissolved oxygen from the water. The aeration time and aeration rate can be adjusted by using a dissolved oxygen meter to monitor the dissolved oxygen concentration in the water in real time.

[0027] Preferably, when the wastewater circulates between the anaerobic reactor and the gas-liquid separator, CO2 is generated intermittently using a CO2 generator and introduced into the gas-liquid separator as needed. This provides a carbon source for the bacteria while adjusting the pH value of the water and controlling the pH value within a suitable range.

[0028] Preferably, when the wastewater circulates between the anaerobic reactor and the gas-liquid separator, the water temperature inside the anaerobic reactor is controlled at 28–35°C using a temperature control system.

[0029] Preferably, in some embodiments of the present invention, the method for evaluating whether a sample to be evaluated contains anaerobic ammonia-oxidizing bacteria specifically includes the following steps:

[0030] (1) Pass clean water into the anaerobic reactor to conduct a trial run, circulating between the anaerobic reactor and the gas-liquid separator, and check for water leakage in the device; at the same time, pass nitrogen into the anaerobic reactor to check the airtightness of the device.

[0031] (2) After confirming that there is no water or air leakage in the device, pull the exhaust pipe that was inserted into the water surface of the water seal bottle out of the water surface.

[0032] (3) Use a feed pump to feed a certain amount of nitrogen-containing wastewater into the anaerobic reactor from the feed tank, and then add the sample to be evaluated into the anaerobic reactor through the feed inlet. Then continue to feed nitrogen-containing wastewater. When the liquid level in the gas-liquid separator is slightly lower than the inlet of the gas-liquid separator, close the feed inlet and insert the exhaust pipe at the water seal bottle into the water in the water seal bottle.

[0033] (4) Start the circulation pump to circulate the wastewater between the anaerobic reactor and the gas-liquid separator.

[0034] (5) Use a temperature control system to control the water temperature in the anaerobic reactor at 28-35℃.

[0035] (6) Nitrogen gas is introduced through the nitrogen inlet to remove dissolved oxygen from the wastewater. When the dissolved oxygen concentration measured by the dissolved oxygen meter no longer decreases, the nitrogen gas introduction is stopped. During the operation of the device, the dissolved oxygen concentration in the water body is monitored in real time using the dissolved oxygen meter 12; nitrogen gas is introduced intermittently to control the dissolved oxygen concentration to be less than 2 μmol / L.

[0036] (7) Use a CO2 generator to introduce CO2 into the gas-liquid separator to provide carbon source for bacteria, and monitor the pH value changes of the pH meter and the gas production in the water seal bottle in real time.

[0037] In some embodiments of the present invention, evaluating the bacterial activity in a sample containing anaerobic ammonia-oxidizing bacteria includes placing the sample containing anaerobic ammonia-oxidizing bacteria in an anaerobic reactor, introducing wastewater containing a known concentration of nitrogen pollutants, circulating the wastewater between the anaerobic reactor and a gas-liquid separator for a period of time, taking a water sample from the anaerobic reactor for analysis, and comparing the analysis results with the concentration of nitrogen pollutants in the initial wastewater to obtain the bacterial activity in the sample to be evaluated.

[0038] Preferably, when the wastewater circulates between the anaerobic reactor and the gas-liquid separator, nitrogen gas is intermittently introduced into the anaerobic reactor and / or the gas-liquid separator to remove dissolved oxygen from the water. The aeration time and aeration rate can be adjusted by using a dissolved oxygen meter to monitor the dissolved oxygen concentration in the water in real time.

[0039] Preferably, when the wastewater circulates between the anaerobic reactor and the gas-liquid separator, CO2 is generated intermittently using a CO2 generator and introduced into the gas-liquid separator as needed. This provides a carbon source for the bacteria while adjusting the pH value of the water and controlling the pH value within a suitable range.

[0040] Preferably, when the wastewater circulates between the anaerobic reactor and the gas-liquid separator, the water temperature inside the anaerobic reactor is controlled at 28–35°C using a temperature control system.

[0041] Preferably, in some embodiments of the present invention, the method for evaluating the activity of bacteria in a sample containing anaerobic ammonia-oxidizing bacteria specifically includes the following steps:

[0042] (1) Pass clean water into the anaerobic reactor to conduct a trial run, circulating between the anaerobic reactor and the gas-liquid separator, and check for water leakage in the device; at the same time, pass nitrogen into the anaerobic reactor to check the airtightness of the device.

[0043] (2) After confirming that there is no water or air leakage in the device, pull the exhaust pipe that was inserted into the water surface of the water seal bottle out of the water surface.

[0044] (3) Prepare wastewater containing a known concentration of nitrogen pollutant and place it in a feed tank. Preferably, the nitrogen pollutant in the wastewater is NH4. + -N and NO2 - -N,NH4 + -N and NO2 - The concentration of -N is known; more preferably, the NH4+ + -N and / or NO2 - The concentration of -N is 30–80 mg / L. More preferably, the NH4+ in the wastewater... + -N and NO2 - The concentration ratio of -N is 1:1 to 1:1.3.

[0045] (4) Use a feed pump to feed a certain amount of wastewater containing a known concentration of nitrogen pollutants into the anaerobic reactor from the feed tank, and then add the sample to be evaluated into the anaerobic reactor through the feed inlet. Preferably, the sample to be evaluated can be dissolved in the wastewater in the feed tank and then sent into the anaerobic reactor. Then continue to feed wastewater containing a known concentration of nitrogen pollutants. When the liquid level in the gas-liquid separator is slightly lower than the inlet of the gas-liquid separator, close the feed inlet and insert the exhaust pipe at the water seal bottle into the water in the water seal bottle.

[0046] (5) Start the circulation pump to circulate the wastewater between the anaerobic reactor and the gas-liquid separator.

[0047] (6) Use a temperature control system to control the water temperature in the anaerobic reactor at 28-35℃.

[0048] (7) Nitrogen gas is introduced through the nitrogen inlet to remove dissolved oxygen from the water. The nitrogen supply is stopped when the dissolved oxygen concentration measured by the dissolved oxygen meter no longer decreases. During device operation, the dissolved oxygen concentration in the water is monitored in real time using a dissolved oxygen meter; nitrogen gas is introduced intermittently to control the dissolved oxygen concentration to be less than 2 μmol / L. Preferably, to quickly remove dissolved oxygen from the water, nitrogen gas can be simultaneously introduced through the air inlet of the gas-liquid separator.

[0049] (8) CO2 is introduced into the gas-liquid separator using a CO2 generator to provide a carbon source for the bacteria, and the pH value of the water in the anaerobic reactor is controlled between 7.4 and 8.0. Preferably, nitrogen is intermittently introduced from the air inlet of the gas-liquid separator to adjust the ratio of CO2 to N2 in the gas-liquid separator.

[0050] (9) After the device has been running for a certain period of time, preferably 2 to 8 hours, turn off the circulation pump and let it stand for a period of time, preferably 2 to 5 hours. Take a sample from the sampling port of the anaerobic reactor for analysis. Compare the results obtained with the concentration of nitrogen pollutants in the initial wastewater to obtain the activity of bacteria in the sample to be evaluated.

[0051] In some embodiments of the present invention, the method for evaluating anaerobic ammonia-oxidizing bacteria includes placing the sample to be evaluated in an anaerobic reactor, introducing wastewater containing a known concentration of nitrogen pollutants, and circulating the wastewater between the anaerobic reactor and a gas-liquid separator for a period of time. The presence of anaerobic ammonia-oxidizing bacteria in the sample is determined by observing pH changes monitored by a pH meter and the venting from the water-sealed bottle. If gas is continuously generated in the water-sealed bottle and the pH meter detects an increase in the water's pH value, the sample to be evaluated is considered to contain anaerobic ammonia-oxidizing bacteria. If the sample contains anaerobic ammonia-oxidizing bacteria, a sample is taken from the bottom sampling port of the anaerobic reactor for analysis. The analysis results are compared with the concentration of nitrogen pollutants in the initial wastewater to determine the bacterial activity in the sample.

[0052] Preferably, when the wastewater circulates between the anaerobic reactor and the gas-liquid separator, nitrogen gas is intermittently introduced into the anaerobic reactor and / or the gas-liquid separator to remove dissolved oxygen from the water. The aeration time and aeration rate can be adjusted by using a dissolved oxygen meter to monitor the dissolved oxygen concentration in the water in real time.

[0053] Preferably, when the wastewater circulates between the anaerobic reactor and the gas-liquid separator, CO2 is generated intermittently using a CO2 generator and introduced into the gas-liquid separator as needed, providing a carbon source for bacteria while adjusting the pH of the water.

[0054] Preferably, in some embodiments of the present invention, the method for evaluating anaerobic ammonia-oxidizing bacteria includes the following steps:

[0055] (1) Pass clean water into the anaerobic reactor to conduct a trial run, circulating between the anaerobic reactor and the gas-liquid separator, and check for water leakage in the device; at the same time, pass nitrogen into the anaerobic reactor to check the airtightness of the device.

[0056] (2) After confirming that there is no water or air leakage in the device, pull the exhaust pipe that was inserted into the water surface of the water seal bottle out of the water surface.

[0057] (3) Prepare wastewater containing a known concentration of nitrogen pollutants and place it in a feed tank. Preferably, the nitrogen pollutant in the wastewater is NH4. + -N and NO2 - -N,NH4 + -N and NO2 - The concentration of -N is known; more preferably, the NH4+ + -N and / or NO2 - The concentration of -N is 30–80 mg / L. More preferably, the NH4+ in the wastewater... + -N and NO2 - The concentration ratio of -N is 1:1 to 1:1.3.

[0058] (4) Use the feed pump to pass a certain amount of wastewater from step (3) into the anaerobic reactor from the feed tank, and then add the sample to be evaluated into the anaerobic reactor through the feed inlet. Then continue to pass the wastewater from step (3). When the liquid level in the gas-liquid separator is slightly lower than the water inlet of the gas-liquid separator, close the feed inlet and insert the exhaust pipe at the water seal bottle into the water in the water seal bottle.

[0059] (5) Start the circulation pump to circulate the wastewater between the anaerobic reactor and the gas-liquid separator.

[0060] (6) Use a temperature control system to control the water temperature in the anaerobic reactor at 28-35℃.

[0061] (7) Nitrogen gas is introduced through the nitrogen inlet to remove dissolved oxygen from the wastewater. The nitrogen supply is stopped when the dissolved oxygen concentration measured by the dissolved oxygen meter 12 no longer decreases. During device operation, the dissolved oxygen concentration in the water body is monitored in real time using the dissolved oxygen meter 12; nitrogen gas is intermittently introduced to control the dissolved oxygen concentration to be less than 2 μmol / L. Preferably, to quickly remove dissolved oxygen from the water body, nitrogen gas can be simultaneously introduced through the air inlet of the gas-liquid separator.

[0062] (8) CO2 is introduced into the gas-liquid separator using a CO2 generator to provide a carbon source for the bacteria, and the pH value of the water in the reactor is controlled between 7.4 and 8.0. Preferably, nitrogen is intermittently introduced from the air inlet of the gas-liquid separator to adjust the ratio of CO2 and N2 in the gas-liquid separator.

[0063] (9) Monitor the pH value changes of the pH meter and the gas production in the water seal bottle in real time. If gas is continuously produced in the water seal bottle during the operation of the device and the pH value of the water body is increased by the pH meter, it indicates that the sample to be evaluated contains anaerobic ammonia-oxidizing bacteria.

[0064] (10) After the device has been running for a certain period of time, preferably 2 to 8 hours, turn off the circulation pump and let it stand for a period of time, preferably 2 to 5 hours. Take a sample from the sampling port of the anaerobic reactor for analysis. Compare the results obtained with the concentration of nitrogen pollutants in the initial wastewater to obtain the activity of anaerobic ammonia oxidizing bacteria in the sample to be evaluated.

[0065] Thirdly, the present invention provides the application of the anaerobic ammonia oxidizing bacteria evaluation device of the first aspect and / or the anaerobic ammonia oxidizing bacteria evaluation method of the second aspect in evaluating anaerobic ammonia oxidizing sludge.

[0066] The beneficial effects of this invention are as follows:

[0067] The anaerobic ammonia oxidation bacteria evaluation device of the present invention is actually a small-scale anaerobic reaction device. A pH meter is installed in the wastewater circulation system consisting of an anaerobic reactor and a gas-liquid separator, and a water seal bottle is connected to the gas outlet of the wastewater circulation system. By monitoring the pH value changes of the water in the wastewater circulation system and the gas production of the anaerobic reaction in real time, it is possible to quickly and easily evaluate whether the reaction occurring in the anaerobic reactor is an anaerobic ammonia oxidation reaction, thereby determining whether the sample to be evaluated contains anaerobic ammonia oxidation bacteria. The operation is simple and the results are accurate.

[0068] The anaerobic ammonia oxidation bacteria evaluation device of the present invention connects the gas outlet of the wastewater circulation system to a water-sealed bottle and also to a gas bag. The gas bag has deformable characteristics, which can reduce the airtightness requirements of the device. In addition, the pressure inside the device can be controlled by controlling the depth of the exhaust pipe inserted into the water surface of the water-sealed bottle, thereby reducing pressure fluctuations within the device. Furthermore, the gas bag can collect and temporarily store N2 separated from the gas-liquid separator (which may be N2 produced by the anaerobic ammonia oxidation reaction or excess N2 introduced into the device from the nitrogen source) and excess undissolved CO2 introduced into the gas-liquid separator from the CO2 generator. This improves the utilization rate of N2 and CO2 and also facilitates the adjustment of the N2 and CO2 ratio within the device.

[0069] The anaerobic ammonia-oxidizing bacteria evaluation device of the present invention reduces the dissolved oxygen content in the water by introducing nitrogen gas into the wastewater circulation system, maintaining an anaerobic or low-oxygen state within the device. This reduces the impact of dissolved oxygen on the anaerobic reaction and improves the accuracy of the evaluation. Furthermore, the device of the present invention also incorporates a temperature control system to regulate the water temperature within the anaerobic reactor, thereby reducing the influence of temperature on the anaerobic reaction and improving the accuracy of the evaluation.

[0070] The anaerobic ammonia-oxidizing bacteria evaluation method of this invention utilizes the anaerobic ammonia-oxidizing bacteria evaluation device of this invention to evaluate the sample to be evaluated. It can be used to quickly evaluate whether the sample contains anaerobic ammonia-oxidizing bacteria, and also to evaluate the activity of bacteria in samples containing anaerobic ammonia-oxidizing bacteria. Furthermore, it is simple to operate and provides accurate results. Applying the anaerobic ammonia-oxidizing bacteria evaluation device and method of this invention to the evaluation of anaerobic ammonia-oxidizing sludge can quickly and accurately determine whether the anaerobic ammonia-oxidizing sludge can be used for anaerobic ammonia oxidation reactions, or effectively assess the activity of anaerobic ammonia-oxidizing bacteria in the sludge. It has excellent application prospects in the field of anaerobic ammonia oxidation denitrification of wastewater. Attached Figure Description

[0071] Figure 1 This is a structural diagram of the anaerobic ammonia-oxidizing bacteria evaluation device of the present invention;

[0072] Figure 2 yes Figure 1 A magnified view of section I in the middle.

[0073] Explanation of reference numerals in the attached diagram:

[0074] 1. Anaerobic reactor; 2. Jacket; 3. Heating tank; 4. Heating pump; 5. Nitrogen inlet; 6. Circulating water inlet; 7. Feed tank; 8. Feed pump; 9. Feed inlet; 10. Circulating water outlet; 11. Gas-liquid separator; 12. Dissolved oxygen meter; 13. CO2 generator; 14. Circulating pump; 15. Water seal bottle; 16. Nitrogen source; 17. Sampling port; 18. Gas bag; 19. pH meter. Detailed Implementation

[0075] The following examples further illustrate the technology of the present invention. These examples are illustrative and exemplary of the present invention and do not limit the scope of the invention in any way.

[0076] Example 1

[0077] An anaerobic ammonia-oxidizing bacteria evaluation device, the evaluation device comprising an anaerobic reactor 1, a jacket 2, a heating tank 3, a heating pump 4, a nitrogen inlet 5, a circulating water inlet 6, a feed tank 7, a feed pump 8, a feed port 9, a circulating water outlet 10, a gas-liquid separator 11, a dissolved oxygen meter 12, a CO2 generator 13, a circulating pump 14, a water seal bottle 15, a nitrogen source 16, a gas bag 18, and a pH meter 19.

[0078] An inlet 9 is provided above one side wall of the anaerobic reactor 1. The inlet 9 is connected to the outlet of the feed tank 7. A feed pump 8 is provided at the outlet of the feed tank 7. The feed pump 8 sends the wastewater containing nitrogen pollutants from the inlet 9 of the anaerobic reactor 1 into the anaerobic reactor 1 for anaerobic denitrification treatment.

[0079] A nitrogen inlet 5 is provided at the bottom of the anaerobic reactor 1. The nitrogen inlet 5 is connected to the outlet of the nitrogen source 16. The nitrogen source 16 is used to provide nitrogen to remove dissolved oxygen in the anaerobic reactor 1.

[0080] A circulating water outlet 10 is provided at the top of the anaerobic reactor 1, and the circulating water outlet 10 is connected to the water inlet provided on the side wall of the gas-liquid separator 11.

[0081] A circulating water inlet 6 is also provided at the bottom of the anaerobic reactor 1, which is connected to the outlet at the bottom of the gas-liquid separator 11. A circulating pump 14 is also provided between the outlet of the gas-liquid separator 11 and the circulating water inlet 6. A dissolved oxygen meter 12 and a pH meter 19 are also provided between the outlet of the gas-liquid separator 11 and the inlet of the circulating pump 14. The dissolved oxygen meter 12 and the pH meter 19 can be used for online monitoring to monitor the dissolved oxygen content and pH value of the circulating water entering the anaerobic reactor 1.

[0082] The circulating pump 14 is a booster pump, which pressurizes the water coming out of the gas-liquid separator 11 and then sends it into the anaerobic reactor 1 through the circulating water inlet 6.

[0083] An air inlet is also provided at the top of the gas-liquid separator 11. The air inlet pipe is a three-way pipe, with one end connected to the air inlet at the top of the gas-liquid separator 11, one end connected to the air outlet of the CO2 generator 13, and the other end connected to the air outlet of the nitrogen source 16, so as to introduce CO2 and N2 into the gas-liquid separator 11.

[0084] An air outlet is provided at the top of the gas-liquid separator 11. The air outlet pipe is a three-way pipe, with one end connected to the air outlet at the top of the gas-liquid separator 11, one end connected to the air bag 18, and the other end connected to the water seal bottle 15.

[0085] The anaerobic ammonia-oxidizing bacteria evaluation device also includes a temperature control system, which comprises a jacket 2, a heating tank 3, and a heating pump 4. The jacket 2 is located on a straight pipe section outside the anaerobic reactor 1. The liquid outlet of the jacket 2 is connected to the liquid inlet of the heating tank 3. The liquid inlet of the jacket 2 is connected to the liquid outlet of the heating tank 3 via the heating pump 4. The liquid heated by the heating tank 3 is pumped from its liquid outlet into the jacket 2 via the heating pump 4. After exchanging heat with the anaerobic reactor 1 inside the jacket 2, the liquid returns to the heating tank 3 from the liquid outlet of the jacket 2.

[0086] Example 2

[0087] An anaerobic ammonia-oxidizing bacteria evaluation device includes an anaerobic reactor 1, a jacket 2, a heating tank 3, a heating pump 4, a nitrogen inlet 5, a circulating water inlet 6, a feed tank 7, a feed pump 8, a feed port 9, a circulating water outlet 10, a gas-liquid separator 11, a dissolved oxygen meter 12, a CO2 generator 13, a circulating pump 14, a water seal bottle 15, a nitrogen source 16, a sampling port 17, a gas bag 18, and a pH meter 19. The structural diagram of this anaerobic ammonia-oxidizing bacteria evaluation device is shown below. Figure 1 As shown.

[0088] An inlet 9 is provided above one side wall of the anaerobic reactor 1. The inlet 9 is connected to the outlet of the feed tank 7. A feed pump 8 is provided at the outlet of the feed tank 7. The feed pump 8 sends the wastewater containing nitrogen pollutants from the inlet 9 of the anaerobic reactor 1 into the anaerobic reactor 1 for anaerobic denitrification treatment.

[0089] A nitrogen inlet 5 is provided at the bottom of the anaerobic reactor 1. The nitrogen inlet 5 is connected to the outlet of the nitrogen source 16. The nitrogen source 16 is used to provide nitrogen to remove dissolved oxygen in the anaerobic reactor 1.

[0090] A circulating water outlet 10 is provided at the top of the anaerobic reactor 1, and the circulating water outlet 10 is connected to the water inlet provided on the side wall of the gas-liquid separator 11.

[0091] A circulating water inlet 6 is also provided at the bottom of the anaerobic reactor 1, which is connected to the outlet at the bottom of the gas-liquid separator 11. A circulating pump 14 is also provided between the outlet of the gas-liquid separator 11 and the circulating water inlet 6. A dissolved oxygen meter 12 and a pH meter 19 are also provided between the outlet of the gas-liquid separator 11 and the inlet of the circulating pump 14. The dissolved oxygen meter 12 and the pH meter 19 can be used for online monitoring to monitor the dissolved oxygen content and pH value of the circulating water entering the anaerobic reactor 1.

[0092] The circulating pump 14 is a booster pump, which pressurizes the water coming out of the gas-liquid separator 11 and then sends it into the anaerobic reactor 1 through the circulating water inlet 6.

[0093] An air inlet is also provided at the top of the gas-liquid separator 11. The air inlet pipe is a three-way pipe, with one end connected to the air inlet at the top of the gas-liquid separator 11, one end connected to the air outlet of the CO2 generator 13, and the other end connected to the air outlet of the nitrogen source 16, so as to introduce CO2 and N2 into the gas-liquid separator 11.

[0094] An air outlet is provided at the top of the gas-liquid separator 11. The air outlet pipe is a three-way pipe, with one end connected to the air outlet at the top of the gas-liquid separator 11, one end connected to the air bag 18, and the other end connected to the water seal bottle 15.

[0095] The anaerobic ammonia-oxidizing bacteria evaluation device also includes a temperature control system, which comprises a jacket 2, a heating tank 3, and a heating pump 4. The jacket 2 is located on a straight pipe section outside the anaerobic reactor 1. The liquid outlet of the jacket 2 is connected to the liquid inlet of the heating tank 3. The liquid inlet of the jacket 2 is connected to the liquid outlet of the heating tank 3 via the heating pump 4. The liquid heated by the heating tank 3 is pumped from its liquid outlet into the jacket 2 via the heating pump 4. After exchanging heat with the anaerobic reactor 1 inside the jacket 2, the liquid returns to the heating tank 3 from the liquid outlet of the jacket 2.

[0096] A sampling port 17 is provided at the lower part of one side wall of the anaerobic reactor 1 to facilitate sampling and analysis.

[0097] Example 3

[0098] A method for evaluating anaerobic ammonia oxidation sludge using the anaerobic ammonia oxidation bacteria evaluation device of Example 1, wherein the method determines whether the sludge to be evaluated is anaerobic ammonia oxidation sludge by observing the pH value change monitored by pH meter 19 and the venting in water seal bottle 15. The method includes the following steps:

[0099] (1) Introduce clean water into the anaerobic reactor 1 to conduct a trial run, and circulate between the anaerobic reactor 1 and the gas-liquid separator 11 to check for water leakage; at the same time, introduce nitrogen into the anaerobic reactor 1 to check the airtightness of the device.

[0100] (2) After confirming that there is no water or air leakage in the device, pull the exhaust pipe inserted into the water seal bottle 15 out of the water.

[0101] (3) Use the feed pump 8 to feed a certain amount of nitrogen-containing wastewater from the feed tank 7 into the anaerobic reactor 1, and then add the sludge to be evaluated into the anaerobic reactor 1 through the feed inlet 9. Then continue to feed wastewater. When the liquid level in the gas-liquid separator 11 is slightly lower than the inlet of the gas-liquid separator 11, close the feed inlet 9 and insert the exhaust pipe at the water seal bottle 15 into the water of the water seal bottle 15.

[0102] (4) Start the circulation pump 14 to circulate the wastewater between the anaerobic reactor 1 and the gas-liquid separator 11.

[0103] (5) Use a temperature control system to control the water temperature in anaerobic reactor 1 to be between 28 and 35°C.

[0104] (6) Nitrogen gas is simultaneously introduced from nitrogen inlet 5 and the air inlet at the top of gas-liquid separator 11 to remove dissolved oxygen from the wastewater. When the dissolved oxygen concentration measured by dissolved oxygen meter 12 no longer decreases, the nitrogen gas is introduced. During the operation of the device, the dissolved oxygen concentration in the water is monitored in real time using dissolved oxygen meter 12; nitrogen gas is introduced intermittently to control the dissolved oxygen concentration to be less than 2 μmol / L.

[0105] (7) CO2 is introduced into the gas-liquid separator 11 using the CO2 generator 13 to provide a carbon source for the bacteria, and the pH value changes of the pH meter 19 and the gas production in the water seal bottle 15 are monitored in real time. Nitrogen is intermittently introduced into the gas-liquid separator 11 through the air inlet to adjust the ratio of CO2 and N2 in the gas-liquid separator.

[0106] If gas is continuously generated in the water seal bottle 15 during the operation of the device, and the pH meter 19 detects an increase in the pH value of the water, it indicates that the sludge to be evaluated is anaerobic ammonia oxidation sludge.

[0107] Example 4

[0108] The method for evaluating anammox sludge using the anammox bacteria evaluation device of Example 2 involves determining whether the sludge to be evaluated is anammox sludge by observing pH changes monitored by pH meter 19 and the venting in water seal bottle 15. When the sludge to be evaluated is determined to be anammox sludge, samples are taken for analysis to evaluate the sludge's denitrification effect. The method includes the following steps:

[0109] (1) Introduce clean water into the anaerobic reactor 1 to conduct a trial run, and circulate between the anaerobic reactor 1 and the gas-liquid separator 11 to check for water leakage; at the same time, introduce nitrogen into the anaerobic reactor 1 to check the airtightness of the device.

[0110] (2) After confirming that there is no water or air leakage in the device, pull the exhaust pipe inserted into the water seal bottle 15 out of the water.

[0111] (3) Prepare a mixture containing NH4 + -N and NO2 - Wastewater containing 80 mg / L of each of -N was placed in feed tank 7.

[0112] (4) Use the feed pump 8 to feed a certain amount of wastewater from step (3) into the anaerobic reactor 1 from the feed tank 7, and then add the sludge to be evaluated into the anaerobic reactor 1 through the feed inlet 9. Then continue to feed the wastewater from step (3). When the liquid level in the gas-liquid separator 11 is slightly lower than the inlet of the gas-liquid separator, close the feed inlet 9 and insert the exhaust pipe at the water seal bottle 15 into the water in the water seal bottle 15.

[0113] (5) Start the circulation pump 14 to circulate the wastewater between the anaerobic reactor 1 and the gas-liquid separator 11.

[0114] (6) Use a temperature control system to control the water temperature in anaerobic reactor 1 to be between 28 and 35°C.

[0115] (7) Nitrogen gas is simultaneously introduced from nitrogen inlet 5 and the air inlet at the top of gas-liquid separator 11 to remove dissolved oxygen from the wastewater. When the dissolved oxygen concentration measured by dissolved oxygen meter 12 no longer decreases, the nitrogen gas is introduced. During the operation of the device, the dissolved oxygen concentration in the water body is monitored in real time using dissolved oxygen meter 12; nitrogen gas is introduced intermittently to control the dissolved oxygen concentration to be less than 2 μmol / L.

[0116] (8) CO2 is introduced into the gas-liquid separator 11 using the CO2 generator 13 to provide a carbon source for the bacteria, and the pH value changes of the pH meter 19 and the gas production in the water seal bottle 15 are monitored in real time. If gas is continuously produced in the water seal bottle 15 during the operation of the device, and the pH value of the water body is increased as monitored by the pH meter 19, it indicates that the sludge to be evaluated is anaerobic ammonia oxidation sludge.

[0117] (9) After the device has been running for 4 hours, the circulation pump 14 is turned off and then left to stand for 3 hours. Samples are taken from the bottom sampling port 17 of the anaerobic reactor 1 for analysis. The results of the analysis are compared with the concentration of nitrogen pollutants in the initial wastewater to obtain the activity of bacteria in the sludge to be evaluated.

[0118] The applicant used the method in Example 4 to evaluate its own sludge containing anaerobic ammonia-oxidizing bacteria. After starting the circulation pump, the device was run continuously for 4 hours. A slight increase in pH value, as monitored by pH meter 19, was observed during operation. Furthermore, gas was continuously emitted from water seal bottle 15 during the operation. These observations indicate that the sludge contains anaerobic ammonia-oxidizing bacteria, which is consistent with the observed results. After a 4-hour small-scale experiment, changes in pH value and gas production are sufficient to determine whether the sludge to be evaluated contains anaerobic ammonia-oxidizing bacteria. This demonstrates that the device is feasible and efficient for identifying sludge or packing materials containing anaerobic ammonia-oxidizing bacteria. After shutting off the circulation pump 14 and allowing it to stand for 3 hours, samples were taken from sampling port 17 of the anaerobic reactor 1 for analysis. The results showed that the sampled wastewater contained NH4+. + -N and NO2 -The concentrations of nitrogen (N) were 50 mg / L, meaning that the evaluated sludge could achieve a nitrogen removal rate of 37.5% after 4 hours of anaerobic ammonia oxidation treatment, indicating that it has good nitrogen removal activity.

[0119] It should be noted that the embodiments described above are only for explaining the present invention and do not constitute any limitation on the present invention. The embodiments describe the present invention, and it should be understood that the terms used therein are descriptive and explanatory terms, not limiting terms. Modifications can be made to the present invention within the scope of the claims, and revisions can be made to the present invention without departing from the scope and spirit of the present invention.

Claims

1. An evaluation device for anaerobic ammonia-oxidizing bacteria, characterized in that, The evaluation device includes an anaerobic reactor (1), a gas-liquid separator (11), a CO2 generator (13), and a gas bag (18). The top of the gas-liquid separator (11) is provided with an outlet and an inlet. The outlet is connected to the gas bag (18), and the inlet is connected to the CO2 generator (13). The anaerobic reactor (1) is provided with a circulating water outlet (10) at the top and a circulating water inlet (6) at the bottom; the circulating water outlet (10) and the circulating water inlet (6) are respectively connected to the inlet and outlet of the gas-liquid separator (11) to realize water circulation between the anaerobic reactor (1) and the gas-liquid separator (11); A pH meter (19) is also installed between the circulating water inlet (6) and the gas-liquid separator (11), and a water seal bottle (15) is also connected to the gas outlet of the gas-liquid separator (11); a sampling port (17) is also installed on the anaerobic reactor (1). The device also includes a nitrogen source (16), and a nitrogen inlet (5) connected to the nitrogen source (16) is provided at the bottom of the anaerobic reactor (1). A dissolved oxygen meter (12) is also provided between the circulating water inlet (6) and the gas-liquid separator (11). The gas inlet of the gas-liquid separator (11) is also connected to the nitrogen source (16).

2. The anaerobic ammonia-oxidizing bacteria evaluation device according to claim 1, characterized in that, The inlet and outlet of the gas-liquid separator (11) are the same; and / or the sampling port (17) is located at the lower part of one side wall of the anaerobic reactor (1).

3. The anaerobic ammonia-oxidizing bacteria evaluation device according to claim 1, characterized in that, The outlet of the gas-liquid separator (11) is lower than the inlet; and / or, a circulation pump (14) is also provided between the gas-liquid separator (11) and the circulating water inlet (6).

4. The anaerobic ammonia-oxidizing bacteria evaluation device according to any one of claims 1-3, characterized in that, The evaluation device also includes a feed tank (7), and an inlet (9) connected to the feed tank (7) is provided on the anaerobic reactor (1).

5. The anaerobic ammonia-oxidizing bacteria evaluation device according to claim 4, characterized in that, The feed tank (7) is also equipped with a feed pump (8) at the outlet. The inlet of the feed pump (8) is connected to the outlet of the feed tank (7), and the outlet of the feed pump (8) is connected to the feed inlet (9).

6. The anaerobic ammonia-oxidizing bacteria evaluation device according to any one of claims 1-3, characterized in that, The device also includes a temperature control system, which includes a jacket (2) disposed outside the anaerobic reactor (1) and a heating tank (3) connected thereto.

7. A method for evaluating anaerobic ammonia-oxidizing bacteria, characterized in that, The method uses the anaerobic ammonia-oxidizing bacteria evaluation device according to any one of claims 1-6 to evaluate whether the sample to be evaluated contains anaerobic ammonia-oxidizing bacteria and to evaluate the activity of bacteria in the sample containing anaerobic ammonia-oxidizing bacteria. The method for evaluating whether the sample to be evaluated contains anaerobic ammonia oxidizing bacteria includes placing the sample to be evaluated in an anaerobic reactor (1), introducing wastewater containing nitrogen pollutants, and circulating the wastewater between the anaerobic reactor (1) and the gas-liquid separator (11) for a period of time. The method for evaluating whether the sample to be evaluated contains anaerobic ammonia oxidizing bacteria is determined by observing the pH value changes monitored by the pH meter (19) and the exhaust situation in the water seal bottle (15). If gas is continuously generated in the water seal bottle (15) and the pH value of the water body is increased by the pH meter (19), it indicates that the sample to be evaluated contains anaerobic ammonia oxidizing bacteria. The evaluation of the bacterial activity in the sample containing anaerobic ammonia oxidizing bacteria includes placing the sample containing anaerobic ammonia oxidizing bacteria in an anaerobic reactor (1), introducing wastewater containing a known concentration of nitrogen pollutants, circulating the wastewater between the anaerobic reactor (1) and the gas-liquid separator (11) for a period of time, taking water samples from the anaerobic reactor (1) for analysis, comparing the results obtained with the concentration of nitrogen pollutants in the initial wastewater, and obtaining the bacterial activity in the sample to be evaluated. When the wastewater circulates between the anaerobic reactor (1) and the gas-liquid separator (11), nitrogen is intermittently introduced into the anaerobic reactor (1) and / or the gas-liquid separator (11) to remove dissolved oxygen from the water. When the wastewater circulates between the anaerobic reactor (1) and the gas-liquid separator (11), CO2 is generated intermittently by the CO2 generator and introduced into the gas-liquid separator (11) as needed, providing a carbon source for the bacteria while adjusting the pH of the water.

8. The method for evaluating anaerobic ammonia-oxidizing bacteria according to claim 7, characterized in that, When nitrogen is introduced, the dissolved oxygen concentration in the water body is monitored in real time using a dissolved oxygen meter (12) to adjust the aeration time and aeration rate. And / or, when the wastewater circulates between the anaerobic reactor (1) and the gas-liquid separator (11), the water temperature in the anaerobic reactor (1) is controlled between 28 and 35°C using a temperature control system.

9. The application of the anaerobic ammonia-oxidizing bacteria evaluation device according to any one of claims 1-6 and / or the anaerobic ammonia-oxidizing bacteria evaluation method according to claim 7 or 8 in the evaluation of anaerobic ammonia-oxidizing sludge.

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