Chemiluminescence immunoassay instrument

Abstract

The application discloses a chemiluminescence immunoassay analyzer, which comprises a dispensing unit, an incubation disc unit, a washing disc unit and a detection unit, the number of reaction vessel cups distributed on each circle of the incubation disc unit is N1, fixed transfer operation is adopted and the fixed transfer and stop time is T1, any reaction vessel on the incubation disc unit is respectively returned to the initial position through N1 times of transfer and stop; the number of reaction vessel cups distributed on each circle of the washing disc unit is N2, fixed transfer operation is adopted and the fixed transfer and stop time is T2, any reaction vessel on the washing disc unit is respectively returned to the initial position through N2 times of transfer and stop; wherein, T1*N1=T2*N2, N1 and N2 are both prime numbers, and T1*N1 is the common divisor of the incubation time of each test item. In the whole test process, the incubation disc unit and the washing disc unit respectively perform specific transfer cup number according to specific transfer and stop time, without the need of self-adaptive transfer operation, so that the control process is simple and the test flux is high.

Description

Technical Field

[0001] This invention belongs to the field of in vitro diagnostics, specifically relating to a chemiluminescence immunoassay analyzer. Background Technology

[0002] Currently, the main clinical function of fully automated chemiluminescence immunoassay analyzers is to quantitatively detect the content of various proteins, antigens, and other items in human serum, plasma, whole blood, or urine in vitro, providing accurate and reliable data for the diagnosis, prevention, treatment, and health monitoring of clinical diseases.

[0003] Chemiluminescence immunoassay analyzer is an analytical method that combines luminescence analysis and immunoassay. Currently, magnetic microparticle chemiluminescence is commonly used. This involves adding the sample to be tested, magnetic microparticles, and other reagents to a reaction vessel and incubating the reaction under certain conditions to form a reaction complex. Then, magnetic separation cleaning is used to remove the magnetic microparticles and other interfering substances from the reaction vessel. Finally, an excitation solution (acidic or alkaline) is added for direct measurement.

[0004] Depending on the different characteristics of the test projects, the following different test processes need to be supported.

[0005] (1) One-step test

[0006] like Figure 1 As shown, the one-step method refers to adding reagents only once during the testing process, which is the simplest testing mode. Reagents and samples are added to the reaction vessel to form a reaction solution and mixed thoroughly. The mixed reaction vessel is then placed under constant temperature conditions for incubation for a certain period of time, followed by cleaning and separation. Finally, excitation solution is added to the cleaned and separated reaction vessel, and photometric measurements are taken.

[0007] (2) Two-step method single-wash separation test

[0008] like Figure 2 As shown, the two-step method involves adding reagents (referred to as the first reagent, which may consist of multiple components) to the reaction vessel during the test, forming a reaction solution from the sample, and mixing it thoroughly. The mixed reaction vessel is then placed under constant temperature conditions for incubation for a certain period of time (first incubation). Then, reagents (referred to as the second reagent, which may consist of multiple components) are added to the reaction vessel again and mixed thoroughly. The mixed reaction vessel is then placed under constant temperature conditions for incubation for a certain period of time (second incubation). After incubation, cleaning and separation are performed, followed by the addition of an excitation solution to the cleaned and separated reaction vessel, and then photometric measurement.

[0009] (3) Two-step method with two cleaning and separation tests

[0010] like Figure 3As shown. The two-step method involves two washing steps: adding a portion of reagent (referred to as the first reagent, which may consist of multiple components) to the reaction vessel, forming a reaction solution, and mixing thoroughly. The mixed reaction vessel is then incubated at a constant temperature for a certain time (first incubation) before washing and separation. Then, reagent (referred to as the second reagent, which may also consist of multiple components) is added to the reaction vessel again and mixed thoroughly. The mixed reaction vessel is then incubated at a constant temperature for a certain time (second incubation). After incubation, washing and separation are performed, followed by the addition of an excitation solution to the washed and separated reaction vessel, and then photometric measurement.

[0011] The incubation tray unit and the cleaning tray unit each have two transfer modes. One is a fixed transfer operation, which involves rotating and stopping at fixed intervals (cycles). The number of cups transferred during each fixed rotation and stop time is the same (the transfer distance is fixed), thereby driving the reaction vessel to advance to each operating position in a regular manner. The other is an adaptive transfer operation, which allows the reaction vessel to be rotated to each operating position as needed.

[0012] For the incubation tray unit, the incubation time varies depending on the test item. For incubation times that are integer multiples of a single time, such as 6 min, 12 min, 18 min, 24 min, a fixed transfer operation or an adaptive transfer operation can be used.

[0013] For cleaning trays, the cleaning process is fixed for different test items, so cleaning trays generally adopt a fixed transfer operation.

[0014] However, in the testing process, after incubation, the reaction vessel needs to be cleaned and separated in the cleaning tray unit. Especially for test projects that require two-step cleaning and separation, the reaction vessel after cleaning and separation needs to be returned to the incubation tray unit for secondary incubation. Therefore, the cleaning tray unit and the incubation tray need to be controlled in coordination. In order to solve the above problems, the incubation tray unit often needs to adopt an adaptive transfer operation to solve this problem, which makes the control process of the incubation tray unit complicated. Summary of the Invention

[0015] The purpose of this invention is to provide a chemiluminescence immunoassay analyzer with a simple control process and high testing throughput.

[0016] The technical solution adopted in this invention is as follows: a chemiluminescence immunoassay analyzer, comprising:

[0017] The dispensing unit injects reagents and / or samples into the reaction vessel;

[0018] The detection unit is used to detect analytes inside the reaction vessel;

[0019] An incubation tray unit is used to incubate the liquid in the reaction vessel. The incubation tray unit includes at least one ring, and the number of reaction vessel cups distributed on each ring is N1. The incubation tray unit adopts a fixed transfer operation, and the fixed rotation and stop time of the incubation tray unit is T1. Any reaction vessel on the incubation tray unit returns to its initial position after N1 rotations and stops.

[0020] A cleaning disc unit is used to remove unbound components from the reaction system in the reaction vessel. The cleaning disc unit includes at least one ring, and the number of reaction vessel cups distributed on each ring is N2. The cleaning disc unit adopts a fixed transfer operation, and the fixed rotation and stop time of the cleaning disc unit is T2. Any reaction vessel on the cleaning disc unit returns to its initial position after N2 rotations and stops.

[0021] Where T1*N1=T2*N2, N1 and N2 are both prime numbers, and T1*N1 is the common divisor of the incubation time of each test item.

[0022] Preferably, T1 = T2, N1 = N2, and each functional station, such as the sample dispensing unit, reagent dispensing unit, and reaction vessel transfer mechanism, repeats the same logical actions in each cycle, ensuring accurate measurement and high CV.

[0023] Preferably, T1*N1 is the greatest common divisor of the incubation time for each test item, which ensures that the number of reaction vessel cups distributed on each ring of the incubation tray unit and the cleaning tray unit is large.

[0024] Preferably, the incubation tray unit includes at least two rings, and each ring of incubation trays starts to rotate and stop synchronously according to a fixed rotation and stop time T1.

[0025] Preferably, the chemiluminescence immunoassay analyzer further includes a reaction vessel supply unit for providing reaction vessels to be used.

[0026] More preferably, the chemiluminescence immunoassay analyzer further includes a reaction container transport mechanism for transporting the reaction container between the reaction container supply unit, the incubation tray unit, and the cleaning tray unit. The reaction supply unit has a reaction container supply unit gripper position, the incubation tray has an incubation tray gripper position, and the cleaning tray has a cleaning tray gripper position. The reaction container supply unit gripper position, the incubation tray gripper position, and the cleaning tray gripper position are all located on the movement trajectory of the reaction container transport mechanism.

[0027] Preferably, the cleaning tray unit is sleeved on the outside or inside of the incubation tray unit and coaxially arranged. The cleaning tray unit is provided with a detection position, and the detection unit is correspondingly arranged on the detection position for detecting the analytes in the reaction vessel at the detection position.

[0028] Preferably, the cleaning tray unit is located outside the incubation tray unit, and the detection unit is correspondingly disposed on the detection position and located outside the cleaning tray.

[0029] Preferably, the chemiluminescence immunoassay analyzer further includes a mixing unit for mixing the liquid in the reaction solution, and the incubation tray unit is provided with a mixing operation position, the mixing unit being used to mix the liquid in the reaction container at the mixing operation position.

[0030] Preferably, the reagent dispensing unit includes a sample dispensing unit for dispensing samples into the reaction vessel, and the incubation tray unit is provided with a sample dispensing operation position. The sample dispensing unit is used to dispense samples into the reaction vessel at the sample dispensing operation position.

[0031] Preferably, the reagent dispensing unit further includes a reagent dispensing unit for adding reagents into the reaction vessel, and the incubation tray unit is provided with a reagent dispensing operation position. The reagent dispensing unit is used to add reagents into the reaction vessel at the reagent dispensing operation position.

[0032] The beneficial effects of the present invention are as follows: In the chemiluminescence immunoassay analyzer of the present invention, the incubation tray unit and the cleaning tray unit can both return to their original positions after the same time (T1*N1), which ensures that the incubation tray unit and the cleaning tray unit can achieve the test process of all test items with incubation time being an integer multiple of a single time by using a fixed transfer operation. Moreover, in the entire test process, the incubation tray unit and the cleaning tray unit each transfer a specific number of cups (transfer distance) according to a specific transfer and stop time, without the need for adaptive transfer operation, the control process is simple, and the test throughput is high. Attached Figure Description

[0033] To more clearly illustrate the technical solution of the present invention, the drawings used in the embodiments will be briefly introduced below. Obviously, those skilled in the art can obtain other drawings based on these drawings without creative effort.

[0034] Figure 1 This is a schematic diagram of the one-step testing process;

[0035] Figure 2 This is a schematic diagram of a two-step single-stage cleaning and separation test process;

[0036] Figure 3 This is a schematic diagram of the two-step method for two-stage cleaning and separation testing.

[0037] Figure 4 This is a schematic diagram of the present invention. Detailed Implementation

[0038] To make the objectives, technical solutions, and advantages of this invention clearer, the technical solutions of this invention will be clearly and completely described below in conjunction with specific embodiments and corresponding drawings. Obviously, the described embodiments are only a part of the embodiments of this invention, and not all of them. Based on the embodiments of this invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this invention. The technical solutions provided by various embodiments of this invention will be described in detail below with reference to the accompanying drawings.

[0039] like Figure 4 As shown, the chemiluminescence immunoassay analyzer includes: an incubation tray unit 1, a dispensing unit, a mixing unit, a washing tray unit 4, a detection unit 5, a reaction container supply unit 6, a reaction container transport unit 7, a sample injection device 8, and a reagent tray 9.

[0040] The incubation tray unit 1 includes a disc-shaped incubation tray, which includes at least one ring. In a specific embodiment of the present invention, the incubation tray has four rings, and each ring is distributed with a certain number of reaction vessel holding cup positions. The number of reaction vessel holding cup positions distributed on each ring of the incubation tray is represented by N1, which is used to incubate the liquid in the reaction vessel. These positions can be holes or grooves, etc., for incubating or / and holding the reaction vessel and transferring the reaction vessel to a predetermined operating position so that other units can perform corresponding operations. The operating position is a specific position on the reaction tray where the reaction vessel can be transferred, such as the sample dispensing operating position 101 for adding samples, the reagent dispensing operating position 102 for adding reagents, the incubation tray cup grasping position 103 for moving the reaction cup from or out of the incubation tray, and the mixing operating position for mixing the liquid in the reaction solution (not shown in the figure). The number of operating positions can be more or less than the above-mentioned operating positions.

[0041] The incubation tray unit 1 employs a fixed transfer operation, meaning it rotates and stops at fixed intervals, transferring the same number of cups each time. The rotation and stopping time of the incubation tray unit is denoted by T1. The number of cups transferred each time can be determined based on specific circumstances, such as the positions of each operating unit. Any reaction vessel on the incubation tray unit returns to its initial position after N1 rotations and stops. For example, a reaction vessel at the initial position during testing (the cup-grabbing position on the incubation tray) returns to its initial position at the start of the test (the cup-grabbing position on the incubation tray) after N1 rotations and stops. In this invention... In a specific embodiment, the number of reaction container cups N1 distributed on each incubation tray is 41, the rotation and stopping time T1 of incubation tray unit 1 is 9s, the incubation tray rotates and stops once every 9s, and each rotation passes 16 cups. The reaction container at the initial position (cup-grabbing position of the incubation tray) at the beginning of the test returns to the initial position (cup-grabbing position of the incubation tray) at the beginning of the test after 41 rotations and stops. That is to say, the reaction container at the initial position (cup-grabbing position of the incubation tray) at the beginning of the test returns to the initial position (cup-grabbing position of the incubation tray) at the beginning of the test after 9s*41 (6min).

[0042] The dispensing unit includes a sample dispensing unit 201 and a reagent dispensing unit 202. The sample dispensing unit 201 includes a sample needle for aspirating and dispensing samples, which aspirates samples from the injection device 8 to dispense samples into the reaction vessel at the sample dispensing operation position 101; the reagent dispensing unit 202 may be a reagent needle for aspirating and dispensing reagents, which aspirates reagents from the reagent tray 9 to dispense reagents into the reaction vessel at the reagent dispensing operation position 102.

[0043] The mixing unit (not shown in the figure) is disposed at the mixing operation position and is used to mix the liquid in the reaction vessel at the mixing operation position. In this embodiment, the mixing unit can be four independent mixing mechanisms, or the same mixing mechanism can simultaneously mix the liquid in the reaction vessel at the mixing operation position in each incubation tray.

[0044] Among them, the sample dispensing unit 201, the reagent dispensing unit 202, and the mixing unit are operation units; each time the incubation tray rotates and stops, each operation unit performs the corresponding operation. That is to say, each time the incubation tray rotates and stops, the sample dispensing unit 201, the reagent dispensing unit 202, the mixing unit, and the reaction vessel transport unit 7 respectively perform sample dispensing, reagent dispensing, mixing, and cup grabbing.

[0045] The cleaning disc unit 4 includes a disc-shaped cleaning disc, which is sleeved on the outside of the incubation disc and coaxially arranged. The cleaning disc and the incubation disc operate independently, saving space and avoiding mutual interference between cleaning separation and incubation. The cleaning disc may include multiple rings, at least one ring. In a specific embodiment of the present invention, the cleaning disc is set to only one ring, with a certain number of reaction vessel cup-carrying positions distributed around the circumference. These positions may be holes or slots, etc., used to carry the reaction vessels and transfer them to predetermined operating positions. The operating positions include a cleaning disc cup-grabbing position 401 for moving the reaction cups in or out of the cleaning disc, a cleaning operation position for removing unbound components in the reaction system within the reaction vessel, an activation liquid injection operation position for injecting activation liquid into the reaction vessel, and a detection position 402 for detecting analytes within the reaction vessel. Specifically, the cleaning operation position includes multiple cleaning fluid injection points (not shown in the figure) and multiple cleaning fluid drainage points (not shown in the figure), arranged alternately. Each of the multiple cleaning fluid injection points is equipped with multiple injection needles (not shown in the figure) for injecting cleaning fluid into the reaction cup at the injection point. Each of the multiple cleaning fluid drainage points is equipped with multiple drainage needles (not shown in the figure) for draining the liquid from the reaction cup at the drainage point. Magnetic components are installed at both the cleaning fluid injection and drainage points to ensure that magnetic beads inside the reaction cup gather on one side of the magnetic component at the corresponding operation position, thereby achieving thorough dispersion and effective cleaning of the magnetic beads during reaction cup transfer. The activation solution injection operation position includes an acidic activation solution injection operation position (not shown in the figure) and an alkaline activation solution injection operation position (not shown in the figure); an acidic activation solution injection mechanism (not shown in the figure) is correspondingly provided at the acidic activation solution injection operation position for injecting acidic activation solution into the reaction cup at the acidic addition position, and an alkaline activation solution injection mechanism (not shown in the figure) is correspondingly provided at the alkaline activation solution injection operation position for injecting alkaline activation solution into the reaction cup at the alkaline addition position.

[0046] The cleaning tray unit adopts a fixed transfer operation, that is, it is rotated and stopped at fixed intervals. The same number of cups are transferred within each fixed rotation and stop time. The number of cups transferred by the cleaning tray within each fixed rotation and stop time can be the same as the number of cups transferred by the incubation tray, or it can be different, depending on the situation. The rotation and stop time of the cleaning tray unit is represented by T2. Any reaction container on the cleaning tray unit returns to its initial position after N2 rotations and stops. For example, the reaction container at the initial position during the test (the cup-grabbing position of the cleaning tray) returns to its initial position at the start of the test (the cup-grabbing position of the cleaning tray) after N2 rotations and stops. In a specific embodiment of the present invention, the number of reaction containers N2 distributed on each rotation of the cleaning tray is 41, and the rotation and stop time T2 of the cleaning tray unit is 9s. The cleaning tray rotates and stops once every 9s, and 5 cups are transferred at a time. The reaction container at the initial position during the test (the cup-grabbing position of the cleaning tray) returns to its initial position at the start of the test (the cup-grabbing position of the cleaning tray) after 41 rotations and stops. That is to say, the reaction container at the initial position during the test (the cup-grabbing position of the cleaning tray) returns to its initial position at the start of the test (the cup-grabbing position of the cleaning tray) after 9s*41 (6min). Each time the cleaning tray stops rotating, each operating unit performs a corresponding operation. These operating units include an injection needle, a drainage needle, an acidic activation solution injection mechanism, an alkaline activation solution injection mechanism, and a detection unit 5. Each time the cleaning tray stops rotating, the injection mechanism, drainage mechanism, acidic activation solution injection mechanism, alkaline activation solution injection mechanism, and detection unit 5 respectively perform cleaning solution injection, cleaning solution drainage, acidic activation solution injection, alkaline activation solution injection, and detection operations.

[0047] The detection unit 5 is correspondingly disposed on the detection position 402 and located outside the cleaning tray. It is used to detect the analyte in the reaction vessel at the detection position 402, and the concentration of the analyte is detected by detecting the light intensity.

[0048] The reaction vessel supply unit 6 is used to supply reaction vessels for use.

[0049] The reaction vessel transfer mechanism 7 transports the reaction vessel between the reaction vessel supply unit, the incubation tray unit, and the cleaning tray unit. The cup-grabbing positions 601 of the reaction vessel supply unit, 103 of the incubation tray, and 401 of the cleaning tray are all located on the movement trajectory of the reaction vessel transfer mechanism 7.

[0050] In this embodiment, the chemiluminescence immunoassay analyzer includes only one reaction container transfer mechanism 7, which simultaneously completes the transfer of the reaction container between the reaction container supply unit cup position 601 and the incubation tray cup position 103, and between the incubation tray cup position 103 and the cleaning tray cup position 401, greatly simplifying the instrument structure.

[0051] This invention arranges the incubation tray unit 1 and the cleaning tray unit 4 coaxially, with the detection position 402 located on the cleaning tray unit 4. This integrates incubation, cleaning, and measurement into a single process. After incubation and cleaning are completed, signal reagents are added directly to complete the detection, eliminating the need to add signal reagents and then transport the device to a location outside the cleaning tray unit for further testing. This design occupies less space, simplifies the structure, and saves testing time.

[0052] The chemiluminescence immunoassay analyzer in this embodiment is suitable for testing items with incubation times of 6 minutes or multiples of 6 minutes, such as 6 minutes, 12 minutes, 18 minutes, 24 minutes, etc.

[0053] For the one-step cleaning test process, the incubation tray 1 transfers an empty slot to the incubation tray cup holder 103. The reaction container is then transported to the incubation tray cup holder 103 via the reaction container transfer mechanism 7. The incubation tray rotates once every 9 seconds. During the first 3 rotation stops, sample addition, reagent addition, and mixing operations can be performed sequentially. After 41 rotation stops, or 6 minutes (depending on the test item; if the incubation time is 12 minutes, then 82 rotation stops are performed; if the incubation time is 18 minutes, then 123 rotation stops are performed; if the incubation time is 24 minutes, then 164 rotation stops are performed), the incubation tray 1 just transfers the reaction container that has finished incubation to the initial incubation tray cup holder 103. The reaction container transfer mechanism 7 transfers the incubated reaction container from the incubation tray cup holder 103 to the cleaning tray cup holder 401. The cleaning tray rotates once every 9 seconds. During each rotation stop, cleaning, addition of acidic activation solution, and addition of alkaline activation solution are performed sequentially to complete the final measurement.

[0054] For the two-step single-wash separation test procedure, incubation tray 1 transfers an empty space to incubation tray cup position 103. The reaction container is then transported to incubation tray cup position 103 via reaction container transfer mechanism 7. The incubation tray rotates once every 9 seconds. During the first three rotation stops, sample addition, first reagent addition, and mixing can be performed sequentially. After 41 rotation stops (9 seconds each, or 6 minutes) (depending on the test item; if the first incubation time is 12 minutes, then 82 rotation stops are performed; if the first incubation time is 18 minutes, then 123 rotation stops are performed; if the first incubation time is 24 minutes, then 164 rotation stops are performed), incubation tray 1 has just transferred the reaction container from the first incubation to the initial incubation tray cup position 103, marking the end of the first incubation period. The reaction vessel, driven by the incubation tray, undergoes sequential addition of the second reagent and mixing during rotation and stop. After 41 rotation and stop times of 9 seconds each (6 minutes in total, depending on the test item; if the second incubation time requires 12 minutes, then 82 rotation and stop times will result in 123 rotation and stop times; if the second incubation time requires 24 minutes, then 164 rotation and stop times will result in 164 rotation and stop times), the incubation tray 1 just transfers the reaction vessel that has completed the second incubation to the initial incubation tray cup-holding position 103. After the second incubation time ends, the reaction vessel transfer mechanism 7 transfers the incubated reaction vessel from the incubation tray cup-holding position 103 to the cleaning tray cup-holding position 401. The cleaning tray rotates and stops every 9 seconds. During each rotation and stop, cleaning, addition of acidic activation solution and alkaline activation solution are performed sequentially to complete the final measurement.

[0055] For the two-step secondary cleaning and separation test procedure, an empty space in incubation tray 1 is transferred to the incubation tray cup position. The reaction container is then transported to the incubation tray cup position 103 via the reaction container transfer mechanism 7. The incubation tray rotates once every 9 seconds. During the first 3 rotation stops, sample addition, first reagent addition, and mixing can be performed sequentially. After 41 rotation stops (6 minutes), the process is complete (depending on the test item; if the first incubation time requires 12 minutes, then after 82 rotation stops; if the first incubation time requires 18 minutes...). If the first incubation time requires 24 minutes, then after 123 cycles of switching and stopping (or 164 cycles of switching and stopping), the incubation tray 1 will have just transferred the reaction vessel that has completed the first incubation to the initial incubation tray cup-grabbing position 103. The first incubation time ends, and the reaction vessel transfer mechanism 7 transfers the incubated reaction vessel from the incubation tray cup-grabbing position 103 to the cleaning tray cup-grabbing position 401. The cleaning tray rotates once every 9 seconds, and each rotation and stop performs the corresponding cleaning operation. After 41 cycles of switching and stopping, the cleaning tray... At that time, the cleaning tray just transferred the reaction vessel that had just finished the first cleaning to the initial cleaning tray cup gripping position 401. The reaction vessel transfer mechanism 7 then transferred the cleaned reaction vessel from the cleaning tray cup gripping position 401 to the incubation tray cup gripping position 103. Driven by the incubation tray, as the incubation tray continues to rotate and stop, the second reagent is added and mixed sequentially. After another 41 rotation-stop times (9 seconds, or 6 minutes) (depending on the test item; if the second incubation time requires 12 minutes, then after 82 rotation-stop times...), the reaction vessel will have completed the process. After 123 cycles of switching and stopping, if the second incubation time requires 24 minutes, then after 164 cycles of switching and stopping, the reaction vessel that has completed the second incubation will be transferred from the incubation vessel at the initial incubation vessel gripping position 103. When the second incubation time ends, the reaction vessel transfer mechanism will transfer the incubated reaction vessel from the incubation vessel gripping position 103 to the cleaning vessel gripping position 401. The cleaning vessel rotates once every 9 seconds. Each time it rotates and stops, it will be cleaned, and acidic and alkaline activation solutions will be added to complete the final measurement.

[0056] Every 9 seconds, during the switching cycle, each functional station, such as sample dispensing unit 201, reagent dispensing unit 202, reaction vessel transfer mechanism 7, liquid injection mechanism, liquid drainage mechanism, acidic activation solution injection mechanism, alkaline activation solution injection mechanism, and detection unit 5, repeats the same logical actions, ensuring accurate measurements and high CV. Throughout the entire testing process, the incubation tray unit and cleaning tray unit each perform a specific number of transfer cups (transfer distance) according to a specific switching cycle, eliminating the need for adaptive transfer operations, simplifying the control process, and achieving high test throughput.

[0057] To ensure more accurate measurements, a completely enclosed system is formed to minimize the entry of external light into the photometric position, thus guaranteeing the accuracy of the measurement results. The cleaning disc unit 4 includes an outer shell made of opaque material, which encloses the disc-shaped cleaning disc within it. The outer shell includes a base 401 mounted on a base plate and a top cover 402 covering the base. The upper part of the base has a hollow cavity. The disc-shaped cleaning disc is disposed within the hollow cavity of the base 401 and connected to a driving device. When the driving device rotates, the base 401 remains stationary, and the motor rotates, causing the disc-shaped cleaning disc to rotate within the base, transferring the reaction cup to the corresponding operating position. A photometric through-hole is provided on the side wall of the outer shell. The detection mechanism 5 is installed at the photometric through-hole and detects the luminous intensity of the reaction cup in the detection position through the photometric through-hole.

[0058] The top cover 402 is provided with a reaction cup pick-up and drop opening at the position of the cup gripping position of the cleaning tray. A light-shielding device is provided at the reaction cup pick-up and drop opening to completely cover or open the reaction cup pick-up and drop opening. The specific structure of the light-shielding device can be referred to in patent 20202309691.4.

[0059] In another embodiment of the present invention, the cleaning tray unit is located inside the incubation tray unit, and the detection unit is correspondingly disposed on the detection position and located inside the cleaning tray.

[0060] In another embodiment of the present invention, a separate mixing unit is provided to mix the liquid in the reaction solution.

[0061] In another embodiment of the present invention, the reaction vessel transfer mechanism includes two parts: a first reaction vessel transfer mechanism and a second reaction vessel transfer mechanism. The first reaction vessel transfer mechanism is used only to transport the reaction vessel between the incubation tray unit and the cleaning tray unit. Specifically, the incubation tray cup-grabbing position and the cleaning tray cup-grabbing position are located on the movement trajectory of the first reaction vessel transfer mechanism. The first reaction vessel transfer mechanism can pick up the reaction vessel from the incubation tray cup-grabbing position and transfer it to the cleaning tray cup-grabbing position, or vice versa. The second reaction vessel transfer mechanism is used to transport the reaction vessel supply unit and the incubation tray unit. The reaction vessel supply unit cup-grabbing position and the incubation tray cup-grabbing position are located on the movement trajectory of the second reaction vessel transfer mechanism, which avoids the restriction of the reaction vessel transfer mechanism on the transfer of the incubation tray.

[0062] The above description is merely a preferred embodiment of the present invention and is not intended to limit the invention. Various modifications and variations can be made to the present invention by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the scope of protection of the present invention.

Claims

1. A chemiluminescence immunoassay analyzer, characterized in that, include: The dispensing unit injects reagents and / or samples into the reaction vessel; The detection unit is used to detect analytes inside the reaction vessel; An incubation tray unit is used to incubate the liquid in the reaction vessel. The incubation tray unit includes at least one ring, and the number of reaction vessel cups distributed on each ring is N1. The incubation tray unit adopts a fixed transfer operation, and the fixed rotation and stop time of the incubation tray unit is T1. Any reaction vessel on the incubation tray unit returns to its initial position after N1 rotations and stops. A cleaning disc unit is used to remove unbound components from the reaction system in the reaction vessel. The cleaning disc unit includes at least one ring, and the number of reaction vessel cups distributed on each ring is N2. The cleaning disc unit adopts a fixed transfer operation, and the fixed rotation and stop time of the cleaning disc unit is T2. Any reaction vessel on the cleaning disc unit returns to its initial position after N2 rotations and stops. Where T1*N1=T2*N2, N1 and N2 are both prime numbers, and T1*N1 is the common divisor of the incubation time of each test item.

2. The chemiluminescence immunoassay analyzer according to claim 1, characterized in that, T1 = T2, N1 = N2.

3. The chemiluminescence immunoassay analyzer according to claim 1 or 2, characterized in that, T1*N1 is the greatest common divisor of the incubation time for all test items.

4. The chemiluminescence immunoassay analyzer according to claim 3, characterized in that, The incubation tray unit includes at least two rings, and each ring of incubation trays starts synchronously and stops according to a fixed start-stop time T1.

5. The chemiluminescence immunoassay analyzer according to claim 1, characterized in that, It also includes a reaction vessel supply unit for supplying reaction vessels in use.

6. The chemiluminescence immunoassay analyzer according to claim 5, characterized in that, It also includes a reaction vessel transfer mechanism for transporting the reaction vessel between the reaction vessel supply unit, the incubation tray unit, and the cleaning tray unit. The reaction vessel supply unit has a reaction vessel supply unit cup gripping position, the incubation tray has an incubation tray cup gripping position, and the cleaning tray unit has a cleaning tray cup gripping position. The reaction vessel supply unit cup gripping position, the incubation tray cup gripping position, and the cleaning tray cup gripping position are all located on the movement trajectory of the reaction vessel transfer mechanism.

7. The chemiluminescence immunoassay analyzer according to claim 1, characterized in that, The cleaning tray unit is sleeved on the outside or inside of the incubation tray unit and is coaxially arranged. The cleaning tray unit is provided with a detection position, and the detection unit is correspondingly arranged on the detection position for detecting the analytes in the reaction vessel at the detection position.

8. The chemiluminescence immunoassay analyzer according to claim 7, characterized in that, The cleaning tray unit is located outside the incubation tray unit, and the detection unit is correspondingly disposed on the detection position and located outside the cleaning tray.

9. The chemiluminescence immunoassay analyzer according to claim 1, characterized in that, It also includes a mixing unit for mixing the liquid in the reaction solution. The incubation tray unit is provided with a mixing operation position, and the mixing unit is used to mix the liquid in the reaction container at the mixing operation position.

10. The chemiluminescence immunoassay analyzer according to claim 1, characterized in that, The dispensing unit includes a sample dispensing unit for dispensing samples into the reaction vessel. The incubation tray unit is provided with a sample dispensing operation position, and the sample dispensing unit is used to dispense samples into the reaction vessel at the sample dispensing operation position.

11. The chemiluminescence immunoassay analyzer according to claim 1, characterized in that, The dispensing unit also includes a reagent dispensing unit for adding reagents into the reaction vessel. The incubation tray unit is provided with a reagent dispensing operation position, and the reagent dispensing unit is used to add reagents into the reaction vessel at the reagent dispensing operation position.

Images