A complex bacterial community comprising halomonas sp. and use thereof

Abstract

The present application relates to the field of microbial technology, in particular to a complex microbial flora comprising halomonas, bacillus and alkalinigenes with denitrification function and its application. The halomonas in the complex microbial flora provided by the present application can improve the salt tolerance of the other two strains, and the denitrification capacity of the complex microbial flora is also significantly improved, and can be applied to various conditions simultaneously. The complex microbial flora provided by the present application can solve the problem that it is difficult to remove nitrogen in high-salinity wastewater by biological method; can be applied to denitrification of high-salinity wastewater with acid, neutral and alkaline simultaneously; can treat low-concentration ammonia-nitrogen wastewater and high-concentration ammonia-nitrogen wastewater in high-salinity wastewater; can play a denitrification role in a large temperature range in high-salinity wastewater; can realize simultaneous nitrification and denitrification when treating high-salinity nitrogen-containing wastewater, and can improve the problem of long process flow of traditional biological denitrification technology.

Description

Technical Field

[0001] This invention belongs to the field of microbial technology, and particularly relates to the application of a complex microbial community including Halomonas, specifically a method for treating high-salt nitrogen-containing wastewater with a microbial community. Background Technology

[0002] Excessive nitrogen in water bodies can lead to eutrophication and a series of problems, including drinking water supply safety, posing a serious threat to ecological balance and human health. Therefore, solving the nitrogen problem in water is urgent. Common denitrification technologies can be divided into physical, chemical, and biological methods. Biological methods are widely used due to their good denitrification effect and the absence of secondary pollution. Existing biological denitrification technologies generally involve autotrophic nitrifying bacteria removing NH4+ under aerobic conditions. + -N is converted to NO2 - -N and NO3 - Nitrification (N) is converted into gaseous nitrogen by heterotrophic denitrifying bacteria under anaerobic conditions and discharged from the system. However, since nitrification and denitrification are two independent parts in this technology, and each part has different requirements for process conditions, it leads to problems such as long process flow and high cost. In addition, most traditional biological nitrogen removal technologies are currently only suitable for the treatment of low-concentration ammonia nitrogen wastewater.

[0003] For high-salinity nitrogenous wastewater from industries such as mariculture, food processing, petroleum production, leather tanning, and pharmaceutical manufacturing, biological denitrification technology, which offers effective denitrification without causing secondary pollution, is difficult to apply. This is because excessive salinity leads to high osmotic pressure, causing plasmolysis in microorganisms and damaging bacterial cells. Furthermore, high salinity affects the activity of enzymes secreted by microorganisms, inhibiting microbial metabolism and ultimately reducing or even eliminating their denitrification capacity in high-salinity environments. In addition, most denitrifying bacteria currently available can only effectively perform denitrification in one of the acidic, neutral, or alkaline environments, exhibiting weak resistance to pH fluctuations and failing to adapt to the complex and variable physicochemical properties of wastewater.

[0004] In summary, existing biological denitrification technologies are difficult to apply to high-salinity wastewater and are also unsuitable for treating high-concentration ammonia nitrogen wastewater. Biological denitrification technologies have long process flows and high costs, and it is difficult to achieve simultaneous nitrification and denitrification when treating high-salinity nitrogen-containing wastewater. They also have weak resistance to pH shocks and cannot adapt to the complex and variable physicochemical properties of wastewater. Summary of the Invention

[0005] In view of this, the present invention provides a complex microbial community including Halomonas and its application. The complex microbial community provided by the present invention has a good treatment effect on nitrogen-containing wastewater.

[0006] This invention provides a complex microbial community including Halomonas.

[0007] Preferably, the Halomonas strain is Halomonas with the accession number CCTCC NO:M20221934.

[0008] Preferably, the complex microbial community further includes:

[0009] Bacillus and / or Alcaligenes faecalis.

[0010] Preferably, the Bacillus is deposited at the China Center for Type Culture Collection, with accession number CCTCC AB2019118.

[0011] Preferably, the alkali-producing bacteria are preserved at the China Center for Type Culture Collection, with accession number CCTCC AB2015392.

[0012] This invention provides a microbial agent comprising the complex microbial community described in the above technical solution.

[0013] This invention provides an application of the composite microbial community and / or the microbial agent described in the above-mentioned technical solutions in nitrogen removal.

[0014] Preferably, the denitrification is the removal of nitrogen from the water body; the water body is nitrogen-containing wastewater.

[0015] Preferably, the nitrogen-containing wastewater contains ammonia nitrogen, nitrate nitrogen, and / or nitrite nitrogen;

[0016] The nitrogen concentration in the nitrogen-containing wastewater is 100–1000 mg / L;

[0017] The salinity of the nitrogen-containing wastewater is 15–100 g / L;

[0018] The pH value of the nitrogen-containing wastewater is 5 to 9;

[0019] The temperature of the nitrogen-containing wastewater is 20–40°C.

[0020] Preferably, the nitrogen removal is biological nitrogen removal;

[0021] The nitrogen removal process is selected from nitrification and / or denitrification.

[0022] The nitrogen removal is selected from heterotrophic and / or aerobic conditions.

[0023] This invention provides a nitrogen removal product, the raw materials of which include the compound microbial community and / or the microbial agent described in the above technical solution.

[0024] Preferably, the complex microbial community includes:

[0025] Two of the following: Halomonas, Bacillus, and Alcaligenes faecalis;

[0026] The mass ratio of the two strains was 1:(0.5 to 1.5).

[0027] Preferably, the microbial community includes:

[0028] Halomonas, Bacillus, and Alcaligenes faecalis;

[0029] The mass ratio of the three strains was 1:(0.5–1.5):(0.5–1.5).

[0030] The composite microbial community constructed in this invention can solve the problem of nitrogen removal from high-salt wastewater using biological methods. By adding Halomonas, the salt tolerance of the other two strains can be improved, thus enhancing the overall denitrification capacity of the composite microbial community. Furthermore, the composite microbial community provided by this invention can be applied to various conditions simultaneously. It can be applied to the denitrification of acidic, neutral, and alkaline high-salt wastewater. It can treat both low-concentration and high-concentration ammonia nitrogen wastewater in high-salt wastewater. When treating high-salt nitrogen-containing wastewater, it can achieve simultaneous nitrification and denitrification, which can improve the problem of long process flow in traditional biological denitrification technology.

[0031] Biological Preservation Instructions

[0032] Halomonas venusta J1-11 was deposited on December 12, 2022, at the China Center for Type Culture Collection (CCTCC), Wuhan University, Wuhan, China, with accession number CCTCC NO: M 20221934. Attached Figure Description

[0033] Figure 1 The results of treating acidic, neutral, and alkaline high-salt wastewater for 12 hours using various strains and compounding methods in the embodiments of the present invention are shown.

[0034] Figure 2 The results of treating high-salt wastewater with low, high, and extremely high ammonia nitrogen concentrations for 12 hours are shown in the embodiments of the present invention, using various strains and compounding methods.

[0035] Figure 3 The results of treating high-salt wastewater at 20℃, 30℃, and 40℃ for 12 hours in the embodiments of the present invention are shown. Detailed Implementation

[0036] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only a part of the embodiments of the present invention, and not all of them. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0037] This invention provides a complex microbial community including Halomonas.

[0038] In this invention, the preferred Halomonas venusta is Halomonas J1-11, which has the preservation number CCTCC NO:M20221934.

[0039] In this invention, the complex microbial community preferably further includes: Bacillus and / or Alcaligenes faecalis.

[0040] In this invention, the Bacillus is preferably a strain deposited at the China Center for Type Culture Collection with accession number CCTCC AB 2019118; the Alcaligenes faecalis is preferably a strain deposited at the China Center for Type Culture Collection with accession number CCTCC AB 2015392.

[0041] In this invention, the composite microbial community preferably includes two of the following: Halomonas, Bacillus, and Alcaligenes faecalis; such as Halomonas and Bacillus; Halomonas and Alcaligenes faecalis; Bacillus and Alcaligenes faecalis; the mass ratio of the two strains is preferably 1:(0.5-1.5), more preferably 1:(0.8-1.2), and most preferably 1:1; the mass ratio of any two strains in this invention can be 1:(0.5-1.5).

[0042] In this invention, the composite microbial community preferably includes: Halomonas, Bacillus, and Alcaligenes faecalis; the mass ratio of the three strains is preferably 1:(0.5-1.5):(0.5-1.5), more preferably 1:(0.8-1.2):(0.8-1.2), and most preferably 1:1:1; the mass ratio of any three strains in this invention can be 1:(0.5-1.5):(0.5-1.5).

[0043] This invention provides a microbial agent comprising the complex microbial community described in the above-mentioned technical solution. In this invention, the dosage form of the microbial agent includes granules, liquids, and dry powders, and this invention is not limited thereto. In this invention, the microbial agent also includes the fermentation broth of *Haloxylon ammodendron*, bacterial cells, supernatant, and the active substances contained therein, and this invention is not limited thereto. In this invention, the mass content of *Haloxylon ammodendron* J1-11 in the fermentation broth is preferably 1-20%, more preferably 5-15%, and most preferably 10%.

[0044] This invention provides the application of the aforementioned complex microbial community and / or the aforementioned microbial agent in nitrogen removal.

[0045] In this invention, the nitrogen removal is preferably the removal of nitrogen from water bodies, and more preferably the removal of nitrogen from nitrogen-containing wastewater. In this invention, the nitrogen-containing wastewater preferably contains ammonia nitrogen, nitrate nitrogen, and / or nitrite nitrogen; the nitrogen concentration in the nitrogen-containing wastewater is preferably 100–1000 mg / L. The composite bacterial community provided by this invention has good treatment effects on nitrogen-containing wastewater with different nitrogen contents. In this invention, the nitrogen-containing wastewater is preferably high-salinity nitrogen-containing wastewater, and the salinity (referring to the sodium chloride content in the nitrogen-containing wastewater) is preferably 15–100 g / L. The composite bacterial community provided by this invention has good treatment effects on nitrogen-containing wastewater with different salinities. In this invention, the pH value of the nitrogen-containing wastewater is preferably 5–9. The composite bacterial community provided by this invention has good treatment effects on acidic, neutral, and alkaline nitrogen-containing wastewater. In this invention, the temperature of the nitrogen-containing wastewater is preferably 20–40°C. The composite bacterial community provided by this invention has good treatment effects on nitrogen-containing wastewater at 20–40°C.

[0046] In this invention, the nitrogen removal is preferably biological nitrogen removal; the nitrogen removal is preferably selected from nitrification and / or denitrification, more preferably from simultaneous nitrification and denitrification; the nitrogen removal is preferably selected from heterotrophic and / or aerobic conditions.

[0047] This invention provides a nitrogen removal product, the raw materials of which include the Halomonas bacteria described in this invention, and / or the complex microbial community described in this invention, and / or the microbial agent described in this invention.

[0048] The present invention also provides a nitrogen removal method, which includes using the nitrogen removal product described in the present invention. The nitrogen removal method preferably includes: culturing the strain in 20 mL of LB medium in a shaker at 30°C and 180 rpm for 24 h, then transferring it to 60 mL of LB medium at a volume ratio of 1-10% and culturing it in a shaker at 30°C and 180 rpm for 12 h, collecting the bacterial cells and centrifuging them, then washing them 2-3 times with physiological saline to prepare a cell suspension, and then adding it to wastewater at a volume ratio of 1-10%.

[0049] The composite microbial community provided by this invention can solve the problem of nitrogen removal from high-salt wastewater by biological methods; it can be applied to denitrification of acidic, neutral, and alkaline high-salt wastewater; it can treat both low-concentration and high-concentration ammonia nitrogen wastewater; it has good treatment effect on high-salt nitrogen-containing wastewater over a wide temperature range; and it can achieve simultaneous nitrification and denitrification when treating high-salt nitrogen-containing wastewater, which can improve the problem of long process flow in traditional biological denitrification technology.

[0050] The Halomonas venusta strain used in the following embodiments of the present invention is Halomonas venusta J1-11 with accession number CCTCC NO:M20221934; the Bacillus strain is with accession number CCTCC AB 2019118; and the Alcaligenes faecalis strain is with accession number CCTCC AB 2015392.

[0051] Example

[0052] Acidic, high-salinity wastewater (pH=5)

[0053] The bacterial strain was cultured in 20 mL of LB medium at 30°C and 180 rpm for 24 h. Then, it was transferred at a volume ratio of 1–10% to 60 mL of LB medium and cultured in a shaker at 30°C and 180 rpm for 12 h. The cells were collected by centrifugation and washed 2–3 times with physiological saline to prepare a cell suspension. This cell suspension was added at a volume ratio of 10% to simulated wastewater (initial nitrogen concentration 100 mg / L) and cultured at 20–40°C and 150–220 rpm for 24 h. OD was measured every 4 h. 600 Ammonia nitrogen content, nitrate nitrogen content, and nitrite nitrogen content; bacterial growth rate (OD). 600 ) represents the absorbance of the bacterial solution at a wavelength of 600 nm, NH4 + The NO3- content was determined using Nessler's reagent spectrophotometry. - The NO2 content was determined using ultraviolet spectrophotometry. - The -N content was determined by the N-(1-naphthyl)-ethylenediamine spectrophotometric method.

[0054] The simulated wastewater had a pH of 5 and a salinity of 30 g / L. Ammonium chloride was used as the sole nitrogen source, with an initial ammonia nitrogen concentration of 100 mg / L. The pH of the wastewater was adjusted using sulfuric acid, and the mixture was cultured at 30°C and 180 rpm. When preparing the bacterial strains, cell suspensions of the strains were first obtained according to the above-mentioned scheme, and then the cell suspensions were combined and added to the simulated wastewater. The samples were then tested according to the above-mentioned method.

[0055] Application of the strain in acidic, high-salt wastewater (pH=5) as follows: Figure 1As shown, strains J1-11 (Haloxymonas) and Alcaligenes faecalis (S1-23) cannot adapt to acidic, high-salinity wastewater. Therefore, these two single strains, as well as the 1:1 combination of the two strains, cannot perform denitrification in acidic, high-salinity wastewater. Bacillus (J1-17) can adapt to acidic, high-salinity wastewater, but its denitrification effect is poor, with a removal rate of only about 27%. Adding either a 1:1 combination of Haloxymonas J1-11 and Bacillus, or a combination of Haloxymonas J1-11 and Bacillus... The combination of three strains of *Haloxylon ammodendron* (BAF) in a 1:1:1 ratio significantly improves the denitrification rate and intensity in acidic wastewater compared to single strains of *Bacillus*, *Haloxylon ammodendron*, or *Alcaligenes faecalis*. This is because *Bacillus* can denitrify at pH 5, and during denitrification, the wastewater pH rises. Once the pH reaches a range suitable for the growth and denitrification of the other two strains (*Haloxylon ammodendron* and *Alcaligenes faecalis*), they begin to play their denitrification role, accelerating the denitrification process. The combined bacterial groups of *Haloxylon ammodendron* J1-11 + *Bacillus*, and *Haloxylon ammodendron* J1-11 + *Bacillus* + *Alcaligenes faecalis* can efficiently treat the denitrification problem in acidic, high-salinity wastewater, and the ammonia nitrogen removal rate after combination is significantly improved compared to single strains.

[0056] Neutral high-salinity wastewater (pH=7)

[0057] The simulated wastewater had a pH of 7 and a salinity of 30 g / L. Ammonium chloride was used as the sole nitrogen source, and the initial ammonia nitrogen concentration was 100 mg / L. The wastewater was cultured at 30°C and 180 rpm. Other detection methods and conditions were the same as those described above.

[0058] The results of the strain's application in neutral high-salt wastewater (pH=7) are as follows: Figure 1 As shown, under neutral conditions, J1-11 halometabolites can respond quickly to high-salt environments, resulting in a rapid removal rate. Any combination involving J1-11 halometabolites, such as halometabolites + Bacillus, halometabolites + Alcaligenes faecalis, or halometabolites + Bacillus + Alcaligenes faecalis, can efficiently remove nitrogen from neutral high-salt wastewater. Furthermore, after combination, the ammonia nitrogen removal rate is significantly higher than that of single bacteria due to the synergistic effect of each strain.

[0059] Alkaline, high-salinity wastewater (pH=9)

[0060] The simulated wastewater had a pH of 9 and a salinity of 30 g / L. Ammonium chloride was used as the sole nitrogen source, with an initial ammonia nitrogen concentration of 100 mg / L. Sodium hydroxide was used to adjust the pH of the wastewater. The wastewater was cultured at 30°C and 180 rpm. Other detection methods and conditions were the same as those described above.

[0061] The results of the strain's application in alkaline high-salt wastewater (pH=9) are as follows: Figure 1As shown, the results are similar to those in neutral high-salinity wastewater. *Haloxymonas* + *Bacillus*, *Haloxymonas* + *Alkali-producing Bacillus*, and *Haloxymonas* + *Bacillus* + *Alkali-producing Bacillus* can all efficiently remove nitrogen in alkaline high-salinity wastewater, and the combined effects are significantly better than those of single bacteria.

[0062] In this invention, a complex bacterial group that is highly efficient at denitrifying high-salt wastewater can be obtained by combining J1-11 halomonas bacteria with Bacillus and Alkalophobic bacteria in a 1:1 ratio, or by combining three strains in a 1:1:1 ratio. These complex bacterial groups are applicable to high-salt wastewater in acidic, neutral, and alkaline conditions. Compared with single bacteria, they greatly enhance their ability to resist pH shocks, and their denitrification performance is greatly enhanced through the synergistic effect of each strain.

[0063] The initial ammonia nitrogen concentration was 100 mg / L.

[0064] The simulated wastewater had a pH of 7 and a salinity of 30 g / L. Ammonium chloride was used as the sole nitrogen source, and the initial ammonia nitrogen concentration was 100 mg / L. The wastewater was cultured at 30°C and 180 rpm. Other detection methods and conditions were the same as those described above.

[0065] The application results of the strain in low-concentration ammonia nitrogen wastewater with an initial ammonia nitrogen concentration of 100 mg / L are as follows: Figure 2 As shown, when the initial ammonia nitrogen concentration was 100 mg / L, the removal rate of strain J1-11 was 92%, while the removal rates of Bacillus and Alcaligenes faecalis were 80% and 76%, respectively. When Halomonas J1-11 and Bacillus were mixed in a 1:1 ratio, the ammonia nitrogen removal rate was 99.76%. When Halomonas J1-11 and Alcaligenes faecalis were mixed in a 1:1 ratio, the ammonia nitrogen removal rate was 99.77%. When Halomonas J1-11 was mixed with Bacillus and Alcaligenes faecalis in a 1:1:1 ratio, the ammonia nitrogen removal rate was 99.69%. This indicates that when Halomonas J1-11 is combined with Bacillus and Alcaligenes faecalis in a 1:1 ratio or in a 1:1:1 ratio of three strains, a composite bacterial group with good denitrification effect in high-salt wastewater can be obtained, with ammonia nitrogen removal rate of over 99%. Moreover, the denitrification effect of the composite bacterial group is significantly better than that of single bacteria.

[0066] The initial ammonia nitrogen concentration was 600 mg / L.

[0067] The simulated wastewater had a pH of 7 and a salinity of 30 g / L. Ammonium chloride was used as the sole nitrogen source, with an initial ammonia nitrogen concentration of 600 mg / L. The wastewater was cultured at 30°C and 180 rpm. Other detection methods and conditions were consistent with the above-mentioned detection methods.

[0068] The application effect of the strain in high-salinity wastewater with high ammonia nitrogen concentration (initial ammonia nitrogen concentration of 600 mg / L) is as follows: Figure 2As shown, when the initial ammonia nitrogen concentration is 600 mg / L, the removal rate of halomonas J1-11 combined with two other strains (Bacillus and Alcaligenes faecalis) in a 1:1 or 1:1:1 ratio is above 92%, which is significantly improved compared to the effect of single strains. This indicates that by combining halomonas with Bacillus and Alcaligenes faecalis, a strain that can be applied to the efficient treatment of high-salt wastewater with high ammonia nitrogen concentrations can be obtained.

[0069] The initial ammonia nitrogen concentration was 1000 mg / L.

[0070] The simulated wastewater had a pH of 7 and a salinity of 30 g / L. Ammonium chloride was used as the sole nitrogen source, and the initial ammonia nitrogen concentration was 1000 mg / L. The wastewater was cultured at 30°C and 180 rpm. Other detection methods and conditions were the same as those described above.

[0071] The application effect of the strain in high-salt wastewater with high ammonia nitrogen concentration of 1000 mg / L is as follows: Figure 2 As shown, when the initial ammonia nitrogen concentration was 1000 mg / L, Bacillus had a poor denitrification effect, while Alcaligenes faecalis had a better effect. When Halomonas J1-11 and Alcaligenes faecalis were combined in a 1:1 ratio or when Halomonas J1-11 was combined with Bacillus and Alcaligenes faecalis in a 1:1:1 ratio, the ammonia nitrogen removal rate was higher than 92%, and the ammonia nitrogen removal rate was significantly improved compared with that of single bacteria.

[0072] In this invention, by combining J1-11 Halomonas, Bacillus, and Alcaligenes faecalis in different ways, a compound bacterial group with good denitrification effect can be obtained in the range of low ammonia nitrogen concentration, high ammonia nitrogen concentration, and high ammonia nitrogen concentration. That is, it can treat both low-concentration and high-concentration ammonia nitrogen wastewater in high-salt wastewater, which can greatly improve the problem that traditional biological denitrification technology can only treat low-concentration ammonia nitrogen wastewater.

[0073] The temperature is 20℃

[0074] The simulated wastewater had a pH of 7 and a salinity of 30 g / L. Ammonium chloride was used as the sole nitrogen source, and the initial ammonia nitrogen concentration was 100 mg / L. The strains were cultured at 20°C and 180 rpm. When the strains were combined, they were first activated according to the above-mentioned scheme to obtain cell suspensions. The cell suspensions were then combined and added to the simulated wastewater, and the tests were performed according to the above-mentioned method.

[0075] The strain's performance in treating high-salt, nitrogen-containing wastewater at 20°C is as follows: Figure 3As shown, the removal rate of halomonas J1-11 at 20℃ is about 92%, while Bacillus and Alcaligenes faecalis can hardly remove nitrogen at 20℃. When halomonas J1-11 is compounded with Bacillus or Alcaligenes faecalis in a 1:1 ratio or with Bacillus and Alcaligenes faecalis in a 1:1:1 ratio, the removal rate of ammonia nitrogen at 20℃ is higher than 98%. This indicates that adding halomonas J1-11 can improve the use of compound bacterial groups at lower temperatures. At the same time, when the strains are compounded, their denitrification ability at 20℃ is significantly improved compared with that of a single strain.

[0076] The temperature is 30℃

[0077] The simulated wastewater had a pH of 7 and a salinity of 30 g / L. Ammonium chloride was used as the sole nitrogen source, and the initial ammonia nitrogen concentration was 100 mg / L. The strains were cultured at 30°C and 180 rpm. When the strains were combined, they were first activated according to the above-mentioned scheme to obtain cell suspensions. The cell suspensions were then combined and added to the simulated wastewater, and the tests were performed according to the above-mentioned method.

[0078] The strain's performance in treating high-salt, nitrogen-containing wastewater at 30°C is as follows: Figure 3 As shown, the removal rates of halomonas J1-11, Bacillus, and Alcaligenes faecalis at 30℃ were approximately 92%, 80%, and 76%, respectively. When halomonas J1-11 was combined with Bacillus or Alcaligenes faecalis in a 1:1 ratio, or when halomonas J1-11 was combined with Bacillus and Alcaligenes faecalis in a 1:1:1 ratio, the removal rate of ammonia nitrogen at 30℃ was over 99%. Compared with the denitrification effect of single strains, the denitrification performance of the combined bacterial groups was significantly improved.

[0079] The temperature is 40℃

[0080] The simulated wastewater had a pH of 7 and a salinity of 30 g / L. Ammonium chloride was used as the sole nitrogen source, and the initial ammonia nitrogen concentration was 100 mg / L. The strains were cultured at 40°C and 180 rpm. When the strains were combined, they were first activated according to the above-mentioned scheme to obtain cell suspensions. The cell suspensions were then combined and added to the simulated wastewater, and the tests were performed according to the above-mentioned method.

[0081] The strain's performance in treating high-salt, nitrogen-containing wastewater at 40℃ is as follows: Figure 3As shown, the removal rate of halomonas J1-11 at 40℃ was only about 74%, while the removal rates of ammonia nitrogen by Bacillus and Alcaligenes faecalis at 40℃ were 90% and 91%, respectively. When halomonas J1-11 was mixed with Bacillus or Alcaligenes faecalis in a 1:1 ratio, or halomonas J1-11 was mixed with Bacillus and Alcaligenes faecalis in a 1:1:1 ratio, the removal rate of ammonia nitrogen at 40℃ was as high as about 98%. This indicates that the denitrification performance of halomonas J1-11 mixed with Bacillus and Alcaligenes faecalis is significantly improved compared with that of a single strain.

[0082] In this invention, a complex bacterial community with high efficiency in denitrification of high-salt wastewater can be obtained by combining J1-11 halomonas bacteria with Bacillus and Alcaligenes faecalis in a 1:1 ratio, or by combining three strains in a 1:1:1 ratio. These complex bacterial communities all showed good denitrification performance when treating high-salt nitrogen-containing wastewater at 20℃, 30℃ and 40℃. Compared with single strains, they greatly enhanced their ability to resist temperature shocks and improved their adaptability to temperature. At the same time, they can treat high-salt nitrogen-containing wastewater over a wide temperature range.

[0083] While the invention has been described and illustrated with reference to specific embodiments thereof, such description and illustration are not intended to limit the invention. It will be readily understood by those skilled in the art that various changes may be made to suit particular circumstances, materials, compositions, substances, methods, or processes to the objectives, spirit, and scope of this application without departing from the true spirit and scope of the invention as defined by the appended claims. All such modifications are intended to be within the scope of the appended claims. Although the methods disclosed herein have been described with reference to specific operations performed in a particular order, it should be understood that these operations may be combined, subdivided, or reordered to form equivalent methods without departing from the teachings of the invention. Therefore, unless specifically indicated herein, the order and grouping of operations are not a limitation of this application.

Claims

1. A complex microbial community, comprising Halomonas, Bacillus, and Alcaligenes faecalis; among which: The preservation number of the Halomonas strain is CCTCC NO:M20221934; The Bacillus species is deposited at the China Center for Type Culture Collection (CCTCC) with accession number CCTCC AB 2019118. The alkali-producing bacteria described are preserved at the China Center for Type Culture Collection, with accession number CCTCC AB 2015392.

2. A microbial agent comprising the complex microbial community described in claim 1.

3. The application of the compound microbial community of claim 1 and / or the microbial agent of claim 2 in nitrogen removal.

4. The application according to claim 3, characterized in that, The denitrification refers to the removal of nitrogen from the water body; the water body is nitrogen-containing wastewater.

5. The application according to claim 4, characterized in that, The nitrogen-containing wastewater contains ammonia nitrogen, nitrate nitrogen, and / or nitrite nitrogen; The nitrogen concentration in the nitrogen-containing wastewater is 100~1000 mg / L; The salinity of the nitrogen-containing wastewater is 15~100 g / L; The pH value of the nitrogen-containing wastewater is 5-9; The temperature of the nitrogen-containing wastewater is 20~40℃.

6. A nitrogen removal product, characterized in that, Its raw materials include the complex microbial community as described in claim 1 and / or the microbial agent as described in claim 2.

Images