An automatic cleaning and temperature-adjusting plant tissue culture device and control method

Abstract

The application discloses an automatic cleaning and temperature adjusting plant tissue culture device and a control method. The device comprises a culture tank, a temperature adjusting system and a control system. The culture tank comprises a jacket, an air inlet device and a shearing device. The temperature adjusting system comprises a supply unit, a conveying unit and a temperature adjusting water tank in communication with the supply unit. The supply unit carries multiple temperature adjusting media with different temperatures to be introduced into and discharged from the jacket and / or the temperature adjusting water tank through the conveying unit. The control system controls the temperature adjusting system to select the temperature adjusting medium to be introduced into the jacket to adjust the temperature of the culture device, controls the cleaning system to introduce water into the culture tank through a water inlet of the culture tank to perform water cleaning, and introduces steam into the culture tank through the air inlet device to clean and steam sterilize the culture tank. The automatic cleaning and temperature adjusting plant tissue culture device can clean and steam sterilize the culture tank without dead angle before culture, and can also adjust the culture temperature of the tissue culture device.

Description

Technical Field

[0001] This invention belongs to the field of tissue and cell culture devices, and more specifically, relates to an automatic cleaning and temperature-regulating plant tissue culture device and control method. Background Technology

[0002] A fermenter is an industrial device used for microbial fermentation. In its design and manufacturing, attention should be paid to a tight and reasonable structure that can withstand steam sterilization, has a certain degree of operational flexibility, minimizes internal accessories (avoiding dead corners), has strong material and energy transfer performance, and can be adjusted to facilitate cleaning, reduce contamination, and is suitable for the production of various products while reducing energy consumption.

[0003] Maintaining a suitable temperature during fermentation culture of biological cells is the most crucial step in cell culture, as the effectiveness of temperature control directly impacts cell culture and growth within the fermenter. Most existing bioreactors are equipped with cooling systems, which primarily involve connecting industrial chilled water to the fermenter's cooling pipe inlet. The water flows into the cooling coils within the fermenter's interior, circulating and cooling the cell solution, thus removing heat and regulating the cell solution temperature to the required process culture temperature.

[0004] In existing plant tissue culture processes, the culture vessel must be kept clean and sterile before adding the culture medium and plant tissue seeds. Therefore, the culture vessel must be cleaned and disinfected. However, due to the viscosity of the culture medium, some areas may not be completely cleaned during the cleaning process, which could contaminate the newly added culture medium and affect the plant tissue culture.

[0005] In view of this, the present invention is proposed. Summary of the Invention

[0006] The technical problem to be solved by the present invention is to overcome the shortcomings of the prior art and provide an automatic cleaning and temperature-regulating plant tissue culture device and control method. It can clean the culture tank thoroughly before culture and regulate the temperature of the plant tissue culture process by introducing media of different temperatures to ensure that the temperature in the plant tissue culture tank is suitable for the growth of plant tissue.

[0007] To solve the above-mentioned technical problems, the basic concept of the technical solution adopted by the present invention is as follows:

[0008] A first aspect of the present invention provides an automatic cleaning and temperature-regulating plant tissue culture apparatus, comprising:

[0009] A culture tank, the culture tank including a jacket fitted outside the tank body, and an air inlet device and a shearing device located at the bottom of the tank body;

[0010] A temperature adjustment system, comprising a supply unit, a conveying unit, and a temperature-regulating water tank connected to the supply unit, wherein the supply unit carries multiple temperature-regulating media of different temperatures and introduces them into and out of the jacket and / or temperature-regulating water tank via the conveying unit, thereby adjusting the culture temperature of the plant tissue culture device.

[0011] The cleaning system includes a water inlet on the culture tank and an air inlet branch pipe for introducing steam into the air inlet device;

[0012] The control system is communicatively connected to the culture tank, temperature adjustment system, and cleaning system.

[0013] The control system controls the temperature adjustment system to select the temperature-regulating medium entering the jacket to adjust the temperature of the culture device, controls the cleaning system to introduce water into the culture tank through the water inlet for water cleaning, and controls the steam to be introduced into the culture tank through the air inlet for cleaning and steam sterilization.

[0014] Furthermore, the temperature-regulating water tank is provided with a water-containing cavity, and the water-containing cavity and the jacket are connected to the supply unit through a conveying unit to form a circulation loop; the conveying unit includes an inlet pipe and an outlet pipe that connect the supply unit and the water-containing cavity; a water pump is connected in series in the inlet pipe to convey the water in the water-containing cavity to the jacket.

[0015] Furthermore, the temperature adjustment system also includes: a heat exchange structure, and a central control unit electrically connected to the pneumatic valves connected in series in each branch of the liquid inlet pipe. The central control unit is used to control the ratio of chilled water and high-temperature steam delivered from the liquid inlet pipe to the heat exchange structure of the temperature-regulating water tank; the heat exchange structure is arranged close to the water-containing cavity.

[0016] Furthermore, the supply unit includes multiple media supply pipelines, which are arranged in parallel.

[0017] Hot water supply main pipe, used to supply the medium at the first temperature; high-temperature steam supply main pipe, used to supply the medium at the second temperature; chilled water supply main pipe, used to supply the medium at the third temperature; cooling water supply pipe, used to supply the medium at the fourth temperature; third temperature < fourth temperature < first temperature < second temperature;

[0018] The delivery unit includes multiple parallel medium input branch pipelines and a first main pipeline, with one of the medium delivery branch pipelines connected to the first main pipeline.

[0019] Multiple media input branch lines correspond one-to-one with multiple media supply line lines, allowing the media at a preset temperature to be input into the jacket at one of them.

[0020] Advantageously, the medium supply pipeline can provide fluid media at different temperatures, which are then fed into the jacket through corresponding medium input branch pipelines, thus serving both a temperature regulation function and increasing the selection of media.

[0021] Furthermore, the high-temperature steam supply pipeline has multiple branches, one of which is connected to the air inlet branch pipe, which is connected to the air inlet device. Steam is introduced into the culture tank through the air inlet device to clean and sterilize the culture device.

[0022] Furthermore, the supply unit also includes: multiple media recovery pipelines; the conveying unit also includes: a second main pipe and multiple media output branch pipes, with each media output branch pipe corresponding to a media recovery pipeline, and the media in the jacket is introduced into the media recovery pipeline through the media output branch pipes.

[0023] Multiple output branch lines are selectively connected to the second main line to export the medium inside the jacket; each output branch line is controllably connected to the second main line.

[0024] Advantageously, multiple output branch lines are connected to the jacket through a single main line, reducing the number of outlets on the jacket while ensuring that different media enter the corresponding media recovery line through different branch lines.

[0025] Furthermore, both the input branch line and the output branch line are equipped with on / off valves, which control the on / off state of the input branch line connected to the first main line and / or the output branch line connected to the second main line.

[0026] Advantageously, the on / off valve allows for the selection of which input and output branch lines to be connected, ensuring that the selected input and output branch lines correspond.

[0027] A second aspect of the present invention provides a control method for an automatic cleaning and temperature-regulating plant tissue culture device, applied to the aforementioned automatic cleaning and temperature-regulating plant tissue culture device, the method comprising:

[0028] S100. Before adding culture medium to the culture tank, control the cleaning system to introduce water into the culture tank through the water inlet on the culture tank to clean the culture device.

[0029] S200. After cleaning, drain the used water.

[0030] S300. Repeat steps S100 to S200 at least three times to add culture medium and plant tissue seeds into the culture tank for culture.

[0031] Optionally, step S100 includes at least: the control system controls the air intake device at the bottom of the culture tank to allow air to enter, and simultaneously controls the shearing device to operate, so as to complete the simultaneous cleaning of the shearing device.

[0032] Optionally, in step S300, during the final cleaning, while controlling the water inlet on the culture tank to allow water to enter the culture tank, the air inlet branch valve is opened to allow steam to be introduced into the culture tank through the air inlet device for cleaning and steam sterilization. At the same time, the shearing device is controlled to work to complete the cleaning of the shearing device simultaneously.

[0033] Optionally, in step S300, after the final cleaning is completed, the following steps are included:

[0034] S301. Add culture medium to the culture tank;

[0035] S302. Steam is introduced into the culture tank through the air inlet device for steam sterilization;

[0036] S303, The temperature control and adjustment system regulates the temperature of the culture device to a suitable culture temperature;

[0037] S304. Plant tissue seeds are added for culture, and a temperature control system is used to regulate the temperature during the culture process.

[0038] By adopting the above technical solution, the present invention has the following beneficial effects compared with the prior art.

[0039] 1. In this invention, media of different temperatures are introduced into a jacket outside the culture tank, and there is a spacer between the jacket and the tank body. This can prevent the media from contaminating the culture solution inside the tank, and the introduction of media of different temperatures can maintain the temperature of the plant tissue culture tank suitable for the growth of plant tissues.

[0040] 2. A temperature-regulating water tank is added to the temperature regulation system, and both the temperature-regulating water tank and the jacket are connected to the supply unit. This allows the temperature-regulating water tank and the jacket to form a parallel loop with the supply unit. The temperature-regulating water tank and the jacket can simultaneously circulate and exchange heat medium with the supply unit. Furthermore, the temperature-regulating water tank and the jacket can be connected in series with the supply unit to form a temperature regulation loop, allowing direct circulation between the temperature-regulating water tank and the jacket. This ensures that the temperature regulation loop can independently regulate the temperature of the plant tissue culture tank when the supply source of the temperature regulation system is damaged or under maintenance, thus improving the fault tolerance of the temperature regulation system.

[0041] 3. The present invention provides a steam inlet branch pipe on the jacket, which allows hot steam to be introduced into the jacket of the tank before cultivation, so that the tank can achieve rapid heating and reduce the preheating time of the tank.

[0042] 4. This invention allows water to enter the culture tank through the inlet and steam to be introduced through the air intake device, which can automatically clean and disinfect the culture tank before cultivation, ensuring the smooth progress of subsequent cultivation.

[0043] The specific embodiments of the present invention will now be described in further detail with reference to the accompanying drawings. Attached Figure Description

[0044] The accompanying drawings, as part of this invention, are used to provide a further understanding of the invention. The illustrative embodiments and descriptions of the invention are used to explain the invention, but do not constitute an undue limitation of the invention. Obviously, the drawings described below are merely some embodiments, and those skilled in the art can obtain other drawings based on these drawings without creative effort. In the drawings:

[0045] Figure 1 This is a schematic diagram of a temperature control system for a plant tissue culture vessel in this invention;

[0046] Figure 2 This is a schematic diagram of the pipeline structure of a cascade culture device in this invention;

[0047] Figure 3 This is a schematic diagram of the piping structure of another cascade culture device in this invention;

[0048] Figure 4 This is a schematic diagram of an air intake device connected to a plant tissue culture tank in this invention;

[0049] Figure 5 This is a schematic diagram of an air intake device according to the present invention;

[0050] Figure 6 This is a cross-sectional schematic diagram of an air intake device according to the present invention;

[0051] Figure 7 This is a schematic diagram of the structure in this invention where the shearing device is installed on the bottom wall of the culture tank;

[0052] Figure 8 This is a schematic diagram of the shearing device in this invention;

[0053] Figure 9 This is a schematic diagram of the shearing section of the shearing device in this invention;

[0054] Figure 10 A top view of the distribution structure of the air intake device and the shearing device on the bottom wall of the seed tank in this invention;

[0055] Figure 11 This is a top view of the distribution structure of the air intake device and the shearing device on the bottom wall of the culture tank in this invention.

[0056] In the diagram: 1. Tank body; 11. Bottom wall; 2. Jacket; 21. First main pipeline; 22. Second main pipeline; 5. On / off valve; 70. Air intake device; 71. Air intake section; 72. Aeration section; 73. Conduit; 731. Air intake section; 732. Air outlet section; 733. Transition section; 74. Sealing plate; 75. Quick-release connector; 451. Sealing groove; 51. Interface; 52. Stacked component; 521. Through hole; 53. Wheel hub assembly; 531. Support ring; 532, Groove; 533, Connecting hole; 54, Sealing ring; 8, Air inlet branch pipe; 9, Water inlet; E200, Hot water supply main pipe; E100, Hot water recovery main pipe; B100, Steam supply main pipe; C200, Chilled water supply main pipe; C100, Chilled water recovery main pipe; D200, Cooling water supply main pipe; D100, Cooling water recovery main pipe; C20, Chilled water supply branch pipe; D20, Cooling water supply branch pipe Pipes; E20, Hot water supply branch pipe; C10, Chilled water recovery branch pipe; D10, Cooling water recovery branch pipe; E10, Hot water recovery branch pipe; A1, Supply unit; A2, Conveying unit; A21, Inlet pipe; A22, Outlet pipe; A24, Liquid inlet pipe; A25, Liquid outlet pipe; A3, Temperature regulating water tank; A32, Heat exchange structure; A301, Water holding chamber; 3, Shearing device; 90, Tank cavity; 31, Spacer; 311, Protruding column 312. Flange edge; 32. Shearing section; 321. Pin shaft; 322. Blade assembly; 323. First blade; 324. Second blade; 325. Rotating end; 326. Blade end; 327. Closed side; 328. Unfolding side; 33. Transmission section; 331. Discharge section; 332. Drive section; 333. Discharge port; 34. Power unit; 35. Flow guide; 351. Inlet; 352. Mounting port; 353. Outlet.

[0057] It should be noted that these accompanying drawings and textual descriptions are not intended to limit the scope of the invention in any way, but rather to illustrate the concept of the invention to those skilled in the art by referring to specific embodiments. Detailed Implementation

[0058] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments will be clearly and completely described below with reference to the accompanying drawings. The following embodiments are used to illustrate the present invention, but are not intended to limit the scope of the present invention.

[0059] In the description of this invention, it should be noted that the terms "upper", "lower", "front", "rear", "left", "right", "vertical", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limiting this invention.

[0060] In the description of this invention, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "linking" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium. Those skilled in the art can understand the specific meaning of the above terms in this invention based on the specific circumstances.

[0061] This invention provides an automatic cleaning and temperature-regulating plant cultivation device, such as... Figure 1 , Figure 2 and Figure 3 As shown, this automatic cleaning and temperature-regulating plant culture device is equipped with a piping structure. The plant tissue culture device includes at least two tanks 1 for culturing isolated plant tissues or cells. Specifically, the tanks 1 are connected in parallel, and each tank 1 is individually supplied with air, nutrients, heat, cleaning, and disinfection supplies via piping from a supply source.

[0062] Specifically, the piping structure includes a main pipeline and branch pipelines. The main pipeline is separated from the cascade culture unit.

[0063] The main pipeline connects multiple devices in parallel, and several main pipelines extend in a straight line and are parallel to each other. Each tank 1 is equipped with a branch pipeline for exchanging and circulating substances with the main pipeline. The branch pipeline connects the main pipeline to the cascade culture device and links the main pipeline to the cascade culture device.

[0064] In this embodiment, the pipeline structure connected to the culture device is optimized and arranged according to the interception size. The main pipeline has a large interception size and is used for centralized supply of materials, while the branch pipelines have small interception sizes and are used to introduce materials from the main pipeline into the culture device. The different main pipelines are arranged in parallel to each other, which not only allows multiple branch pipelines to be connected in the extension direction of each main pipeline, but also facilitates quick identification of pipelines during maintenance, thereby improving the regularity of the pipeline structure and reducing the ineffective space occupation of pipelines.

[0065] Specifically, the pipeline structure is equipped with main pipelines for supplying and recovering fluid media at different temperatures, and these main pipelines are connected to form a complete circulation loop. This allows the pipeline structure to exchange heat with the cultivation device, thus providing stable growth conditions for the proliferation of adventitious roots and other detached plant tissues. The parallel arrangement of the different main pipelines makes the pipeline structure neat and easy to maintain.

[0066] In this embodiment of the invention, the automatically cleaning and temperature-regulating plant tissue culture device includes:

[0067] The culture tank includes a jacket 2 fitted outside the tank body 1, an air inlet device 70 and a shearing device 3 located at the bottom of the tank body 1.

[0068] A temperature adjustment system, comprising a supply unit A1, a conveying unit A2, and a temperature-regulating water tank A3 connected to the supply unit A1, wherein the supply unit A1 carries multiple temperature-regulating media of different temperatures, which are introduced into and out of the jacket 2 and / or the temperature-regulating water tank A3 via the conveying unit A2, thereby achieving the adjustment of the culture temperature of the plant tissue culture device.

[0069] The cleaning system includes a water inlet 9 on the culture tank and an air inlet branch pipe 8 that introduces steam into the air inlet device 70;

[0070] The control system is communicatively connected to the culture tank, temperature adjustment system, and cleaning system.

[0071] The control system controls the temperature adjustment system to select the temperature regulating medium introduced into the jacket 2 to adjust the temperature of the culture device, controls the cleaning system to introduce water into the culture tank through the water inlet 9 for water cleaning, and controls the steam to be introduced into the culture tank through the air inlet 70 for cleaning and steam sterilization.

[0072] It should be noted that steam is introduced into the culture tank through the air inlet device 70. This can be done either before the culture medium is added to clean and sterilize the culture tank, or after the culture medium is added to the culture tank, steam is introduced into the culture medium through the air inlet device 70 to sterilize the culture medium.

[0073] In this embodiment, a temperature-regulating water tank A3 is added to the temperature-regulating system, and both the temperature-regulating water tank A3 and the jacket 2 are connected to the supply unit A1. This allows the temperature-regulating water tank A3 and the jacket 2 to form a circuit in parallel with the supply unit A1. The temperature-regulating water tank A3 and the jacket 2 can simultaneously exchange heat media with the supply unit A1. Furthermore, the temperature-regulating water tank A3 and the jacket 2 can be connected in series by the supply unit A1 to form a temperature-regulating circuit, allowing direct circulation between the temperature-regulating water tank A3 and the jacket 2. This ensures that the temperature-regulating circuit can independently regulate the temperature of the plant tissue culture tank when the supply source of the temperature-regulating system is damaged or under maintenance, thus improving the fault tolerance of the temperature-regulating system.

[0074] Furthermore, the temperature-regulating water tank A3 is provided with a water-containing cavity A301. The water-containing cavity A301 and the jacket 2 are connected to the supply unit A1 through the conveying unit A2 to form a circulation loop. The conveying unit A2 includes an inlet pipe A21 and an outlet pipe A22 that connect the supply unit A1 and the water-containing cavity A301. A water pump is connected in series in the inlet pipe A21 to convey the water in the water-containing cavity A301 to the jacket 2.

[0075] Furthermore, the temperature adjustment system also includes: a heat exchange structure A32, and a central control unit electrically connected to the pneumatic valves connected in series in each branch of the liquid inlet pipe A24. The central control unit is used to control the ratio of chilled water and high-temperature steam delivered from the liquid inlet pipe A24 to the heat exchange structure A32 of the temperature-regulating water tank A3; the heat exchange structure A32 is disposed against the water-containing cavity A301.

[0076] The temperature control system is equipped with a temperature-controlled water tank A3 and a heat exchange structure A32.

[0077] Specifically, the temperature-regulating water tank A3 is provided with a water-containing cavity A301 for holding and heating the liquid that can be input into the jacket 2. In addition, the heat exchange structure A32 is disposed against the water-containing cavity A301 and can exchange heat with the liquid in the water-containing cavity A301, thereby forming a circulation loop between the temperature-regulating water tank A3 and the jacket 2 that can supply heat to the jacket 2.

[0078] The water-containing chamber A301 and the jacket 2 are connected to the supply unit A1 via the conveying unit A2 to form a circulation loop. The conveying unit A2 includes an inlet pipe A21 and an outlet pipe A22 that connect the supply unit A1 and the water-containing chamber A301. The temperature of the plant tissue culture tank is maintained by the temperature-regulating water tank A3.

[0079] Furthermore, the supply unit A1 includes multiple media supply pipelines, including a hot water supply main pipe E200 connected in parallel for supplying media at a first temperature; a high-temperature steam supply main pipe B100 for supplying media at a second temperature; a chilled water supply main pipe C100 for supplying media at a third temperature; and a cooling water supply pipe D100 for supplying media at a fourth temperature.

[0080] Third temperature < Fourth temperature < First temperature < Second temperature;

[0081] The conveying unit A2 includes multiple parallel medium input branch pipelines and a first main pipeline 21, with one of the medium input branch pipelines being connected to the first main pipeline 21.

[0082] Multiple media input branch lines correspond one-to-one with multiple media supply line lines, and media at a preset temperature are input into jacket 2 at one of them.

[0083] Specifically, the media supply pipeline includes several parallel main pipelines, and each main pipeline is used to introduce different substances into the plant tissue culture tank or jacket 2.

[0084] Specifically, the medium input branch line is connected to the medium supply line. The medium supply line carries fluid media at different temperatures and is equipped with multiple main supply lines corresponding to different temperature media. When it is necessary to introduce media into the jacket 2, the media is discharged from the corresponding main supply line in the medium supply line and enters the medium input branch line. Then, the medium input branch line introduces the media into the jacket 2.

[0085] Furthermore, the high-temperature steam supply pipeline B100 of the medium supply pipeline is provided with multiple branches, one of which is connected to the air inlet branch pipe 8. The air inlet branch pipe 8 is connected to the air inlet device 70. Steam is introduced into the culture device through the air inlet device 70 to clean and sterilize the culture device.

[0086] like Figure 1 , Figure 2 and Figure 3 As shown, in this embodiment, the multiple media supply pipelines include: a hot water supply main pipe E200 for supplying hot water; a high-temperature steam supply main pipe B100 for supplying high-temperature steam; a chilled water supply main pipe C100 for supplying chilled water; and a cooling water supply pipe D100 for supplying cooling water.

[0087] In this embodiment, the input branch pipeline includes: a hot water supply branch pipeline E20, which is connected to the hot water supply main pipeline E200, and a high-temperature steam supply branch pipeline, which is connected to the high-temperature steam supply pipeline B100, for introducing hot water / high-temperature steam into the first main pipeline 21 and then into the jacket 2 to adjust the culture temperature of the culture device.

[0088] In this embodiment, the input branch pipeline includes: a chilled water supply branch pipeline C20, which is connected to the chilled water supply main pipeline C100, and a cooling water supply branch pipeline D20, which is connected to the cooling water supply main pipeline D200. The chilled water / cooling water is introduced into the first main pipeline 21 and then into the jacket 2 to cool and regulate the culture temperature of the culture device.

[0089] The control system controls the temperature adjustment system, regulating the flow rate ratio of chilled water and high-temperature steam supplied to the temperature-regulating water tank A3 and the heat exchange structure A32. The chilled water and high-temperature steam entering the temperature-regulating water tank A3 or the heat exchange structure A32 can exchange heat with the hot water in the water-containing chamber A301, thus ensuring that the hot water supplied into the jacket 2 meets the requirements of the plant tissue culture tank. Furthermore, even when the supply unit A1 cannot directly supply hot water to the jacket 2, the temperature-regulating water tank A3 can be used to supply hot water to the jacket 2 to maintain the operation of the plant tissue culture tank.

[0090] In another embodiment, the temperature-regulating water tank A3 can be equipped with an electric heating structure to directly heat the liquid in the water-containing chamber A301. Therefore, when hot water cannot be directly supplied to the jacket 2 from the hot water supply main pipe E200, the hot water supply main pipe E200 and the hot water recovery main pipe E100 can be directly connected to the jacket 2 and the temperature-regulating water tank A3. In this way, a separate circulation loop is formed between the temperature-regulating water tank A3 and the jacket 2, enabling the direct supply of hot water from the temperature-regulating water tank A3 to the jacket 2, ensuring the culture tank meets its hot water temperature requirements.

[0091] Specifically, during normal operation of the supply unit A1, the jacket 2 can be supplied with hot water directly from the hot water supply main pipe E200, or simultaneously with hot water from the temperature-regulating water tank A3. In this case, the temperature of the hot water in the water-containing chamber A301 can be further increased before being supplied to the hot water supply main pipe E200, thus providing temperature compensation for the hot water in the main pipe E200 and allowing for more precise adjustment and control of the culture tank's temperature.

[0092] In this embodiment, the temperature-regulating water tank A3 and the plant tissue culture tank are connected in parallel to the supply unit A1. This allows the supply unit A1 to simultaneously supply hot water to both the temperature-regulating water tank A3 and the jacket 2, and also allows the temperature-regulating water tank A3 to supply hot water to the jacket 2. This adds a backup heat source to the temperature regulation system, ensuring that the plant tissue culture tank can maintain and regulate the temperature of the nutrient solution inside the plant tissue culture tank normally when the supply unit A1 is damaged or under maintenance.

[0093] Specifically, the temperature-regulating water tank A3 has a heat exchange structure A32 disposed against the water-containing cavity A301. The conveying unit A2 includes an inlet pipe A24 and an outlet pipe A25 connecting the supply unit A1 and the heat exchange structure A32, for introducing and discharging liquids of different temperatures into and out of the heat exchange structure A32 respectively.

[0094] In this embodiment, by setting a heat exchange structure A32 in the temperature-controlled water tank A3, the hot water in the temperature-controlled water tank A3 is kept at the set temperature during the operation of the supply unit A1. This ensures that the temperature-controlled water tank A3 can immediately supply hot water to the jacket 2 when the supply unit A1 stops supplying or is under maintenance, without waiting for the water in the temperature-controlled water tank A3 to be heated. This ensures that the backup heat exchange circuit of the temperature control system can quickly take over from the main heat exchange circuit to maintain the normal temperature of the plant tissue culture tank.

[0095] An electric heating structure can be installed in the temperature-regulating water tank A3 to directly heat the liquid in the water-containing chamber A301. Therefore, when hot water cannot be directly supplied to the jacket 2 from the hot water supply main pipe E200, the hot water supply main pipe E200 and the hot water recovery main pipe E100 can be directly connected to the jacket 2 and the temperature-regulating water tank A3. This forms a separate circulation loop between the temperature-regulating water tank A3 and the jacket 2, allowing hot water to be directly supplied to the jacket 2 from the temperature-regulating water tank A3, ensuring the culture tank meets its hot water temperature requirements. Specifically, when the supply unit A1 is operating normally, the jacket 2 can be supplied with hot water directly from the hot water supply main pipe E200, or simultaneously with hot water from the temperature-regulating water tank A3. In this case, the temperature of the hot water in the water-containing chamber A301 can be further increased before being supplied to the hot water supply main pipe E200, providing temperature compensation for the hot water in the hot water supply main pipe E200, thereby allowing for more precise adjustment and control of the culture tank temperature.

[0096] In this embodiment of the invention, an air inlet device 70 and a shearing device 3 are provided at the bottom of the tank 1. High-temperature steam can be introduced into the culture device through the air inlet device 70 for cleaning and steam sterilization. Multiple branch pipes are provided on the main steam supply pipe B100, which are respectively connected to the steam supply branch pipe, the air inlet device 70, and the sterilization medium inlet at the bottom of the on / off valve 5 for high-temperature sterilization. While cleaning and steam sterilizing the culture tank, the shearing device 3 is simultaneously operated to clean it, ensuring that the shearing device 3 is cleaned thoroughly without any blind spots.

[0097] like Figure 10 and Figure 11 As shown, specifically, the plant tissue culture apparatus includes a tank 1, an air inlet device 70 disposed at the bottom of the tank 1, and a shearing device 3 disposed above the air inlet device 70. The tank 1 is used to cultivate isolated plant tissues or cells. The air inlet device 70 is used to introduce air / high-temperature steam into the tank 1.

[0098] like Figure 4 , Figure 5 and Figure 6 As shown, the air intake device 70 includes an air intake section 71, an aeration section 72, and a conduit 73 connecting the air intake section 71 and the aeration section 72. The conduit 73 is a straight rod, and the air intake section 71 and the aeration section 72 are respectively connected to the two ends of the conduit 73.

[0099] Specifically, the aeration section 72 has a central cavity. The cavity is connected to the conduit 73, and the cavity wall has micropores that connect from the outside of the aeration section 72 to the inside of the cavity. A large number of micropores are densely and evenly distributed throughout the cavity wall.

[0100] Preferably, the aeration section 72 is made of titanium alloy material, so that adventitious roots cannot adhere to the outer surface of the aeration section 72, thereby avoiding the accumulation and decay of adventitious roots.

[0101] In this embodiment, by densely opening a large number of micropores on the sidewall of the aeration section 72 and connecting all the micropores to the cavity in the center of the aeration section 72, the gas entering the cavity of the aeration section 72 through the air guide pipe can be sprayed into the solution from all the micropores at the same time, thereby forming a large number of small bubbles in the solution. The small bubbles move outward from the outer surface of the aeration section 72 and float upward, avoiding the aggregation of small bubbles into large bubbles, which improves the contact and dissolution rate between the gas and the solution. Furthermore, the dispersed floating movement of a large number of small bubbles in the solution promotes the circulation of the solution and further improves the oxygenation effect.

[0102] Specifically, the aeration section 72, which is equipped with the air intake mechanism, is elongated and cylindrical, with one end of the aeration section 72 connected to the conduit 73. The central axis of the aeration section 72 coincides with the central axis of the end of the conduit 73 and extends in a straight line away from the conduit 73. At the same time, the conduit 73 is long and straight, so that the central axes of the aeration section 72 and the conduit 73 are aligned.

[0103] The seat plate 74 provided on the air intake device 70 is located between the air intake section 71 and the aeration section 72, separating the air intake section 71 and the aeration section 72.

[0104] Preferably, the seat plate 74 is configured as a circular flat plate, so that the air intake device 70 can rotate around the duct 73 during installation.

[0105] In this embodiment, a seat plate 74 is provided on the air intake device 70, which makes it easier to install and connect the air intake device 70 to the plant tissue culture tank, and also allows the air intake device 70 to rotate around the duct 73, reducing the difficulty of assembly work.

[0106] More specifically, the duct 73 includes a long, straight air inlet section 731. The air inlet section 731 extends at least a certain length to both sides of the seat plate 74, thereby facilitating better connection and fixation of the aeration section 72 and the air inlet section 71 to the duct 73.

[0107] The aeration section 72 and the air intake section 71 are respectively connected to the ends of the air intake section 731 that extend out of the side of the seat plate 74, thereby separating the aeration section 72 and the air intake section 71 on both sides of the seat plate 74.

[0108] In another embodiment of the present invention, in order to increase the position of the aeration section 72 in the plant tissue culture tank after it is installed, the conduit 73 is further provided with an air outlet section 732 that is set at an angle to the air inlet section 731.

[0109] One end of the air outlet section 732 is connected to the air inlet section 731, and the other end is connected to the aeration section 72. In particular, the included angle between the air outlet section 732 and the air inlet section 731 is greater than 90°.

[0110] In another embodiment, in order to further expand the position range of the aeration section 72 within the plant tissue culture tank in conjunction with the seat plate 74, the conduit 73 is also provided with a transition section 733 connecting the air inlet section 731 and the air outlet section 732.

[0111] Specifically, a transition section 733 is provided parallel to the seat plate 74. One end of the transition section 733 is connected to the air intake section 731, and the other end is connected to the air outlet section 732. The air outlet section 732 is inclined to the transition section 733 and extends from the transition section 733 in a direction away from the seat plate 74.

[0112] In this embodiment, a quick-release connector 75 is located at the end of the conduit 73 in the air intake section 71. The quick-release connector 75 is conical in shape. Furthermore, the conical bottom surface of the quick-release connector 75 coincides with the air inlet end face of the conduit 73. The air inlet of the conduit 73 is located at the center of the bottom surface. The conical surface of the quick-release connector 75 faces the seat plate 74 and gradually tapers towards the outer circumference of the conduit 73.

[0113] like Figures 4 to 6 As shown, in another embodiment of the present invention, a quick-release connector 75 having a sealed air intake device 70 is described.

[0114] The quick-release connector 75 has a sealing groove 451 on its conical bottom surface. The sealing groove 451 is recessed from the bottom surface into the quick-release connector 75 and forms a notch on the bottom surface. In particular, the sealing groove 451 surrounds the outer periphery of the air inlet of the duct 73.

[0115] The present invention also provides a plant tissue culture vessel having any of the above-described air intake devices 70. The bottom of the plant tissue culture vessel is configured as an inverted cone. An interface 51 for connecting the air intake device 70 is provided on the side wall of the cone. The air intake device 70 is connected to the interface 51, and its aeration section 72 is located inside the cavity of the plant tissue culture vessel, while the air intake section 71 extends out of the outside of the plant tissue culture vessel.

[0116] In this embodiment, by setting the air intake device 70, the plant tissue culture tank can form a large number of small bubbles in the solution when cultivating adventitious roots. The small bubbles move outward and upward from the aeration section 72, promoting the circulation of the nutrient solution. Furthermore, the large number of small bubbles dispersed in the solution further improves the contact and solubility of the gas and the nutrient solution, providing stable conditions for the proliferation of adventitious roots and helping to improve the cultivation efficiency and quality of adventitious roots.

[0117] like Figure 4As shown, in another embodiment of the present invention, an air intake device 70 that can rotate at any angle around its own central axis when connected to a culture tank is described. The mounting assembly includes an annular stacked member 52.

[0118] When the air intake device 70 is fixedly installed, the stacked component 52 and the interface 51 on the culture tank are connected at their end faces and the seat plate 74 is sandwiched between them. Thus, when the stacked component 52 and the interface 51 are not tightly attached, the seat plate 74 can be rotated to adjust the position and orientation of the air intake device 70 relative to the culture tank.

[0119] Specifically, the stacking member 52 is configured as an annular structure with a central annular hole. The stacking member 52 is sleeved with the base plate 74 and its annular hole edge abuts against the outer surface of the base plate 74 facing the plant tissue culture vessel, for clamping the air intake device 70 onto the plant tissue culture vessel.

[0120] In another embodiment, the mounting assembly further includes a hub assembly 53 with a cylindrical outer peripheral surface. The hub assembly 53 is nested in the interface 51 and connected to the interface 51 with its outer peripheral surface. Specifically, the axis of the hub assembly 53 is perpendicular to the surface of the bottom wall 11 of the culture tank and protrudes axially inward and outward from the bottom wall 11.

[0121] Meanwhile, the inner circumferential surface of the wheel hub assembly 53, which forms a central hole, is connected to the seat plate 74.

[0122] Furthermore, a support ring 531 is provided at one end of the hub assembly 53 facing the inner side of the bottom wall 11. The support ring 531 extends radially from the inner circumference of the hub assembly 53 toward the central axis of the hub assembly 53 and forms a through hole 521 through which the aeration section 72 can pass.

[0123] The seat plate 74 is fitted onto the inner circumferential surface of the hub assembly 53 from the outside of the bottom wall 11, and the seat plate 74 abuts against the support ring 531.

[0124] In another embodiment, a mounting assembly with improved sealing is described. This mounting assembly includes a sealing ring 54 for filling gaps between contact surfaces.

[0125] Specifically, the sealing ring 54 is made of rubber, which is elastic and has good extensibility. Furthermore, the sealing ring 54 is disposed between the seat plate 74 and the support ring 531, and is clamped between the end face of the seat plate 74 facing inwards and the end face of the support ring 531.

[0126] Specifically, when the seat plate 74 and the support ring 531 clamp the sealing ring 54, the thickness of the sealing ring 54 is reduced, so that the protrusions and depressions on the end faces of the seat plate 74 and the support ring 531 that are close to each other are filled by the sealing ring 54, thereby improving the sealing performance of the air intake device 70 mounting assembly.

[0127] Furthermore, a support ring 531 is disposed at the end of the wheel hub assembly 53 located within the bottom wall 11. The through hole 521 on the support ring 531 is aligned with the central axis of the central hole of the wheel hub assembly 53. Additionally, one end face of the support ring 531 is flush with the end face of the wheel hub assembly 53, and the other end face is located within the central hole of the wheel hub assembly 53.

[0128] Specifically, a groove 532 is formed on the end face of the support ring 531 located within the central hole of the hub assembly 53. The opening of the groove 532 faces the outer side of the bottom wall 11. The sealing ring 54 is embedded in the groove 532 and protrudes from the end face of the support ring 531. When the intake device 70 is installed and fixed, the seat plate 74 compresses the sealing ring 54, causing the sealing ring 54 to deform and fill the groove 532.

[0129] In this embodiment, the mounting plate 74 of the mounting component is fitted with the wheel hub assembly 53 with a gap. The mounting plate 74 can be flexibly rotated into the center hole of the wheel hub assembly 53. The mounting plate 74 is clamped and fixed in the wheel hub assembly 53 by the pressing member 52 from the other side.

[0130] Specifically, the hub assembly 53 has a connecting hole 533 on its end face protruding from the outer side of the bottom wall 11 of the culture tank. The connecting hole 533 extends along the axial direction of the hub assembly 53 and is used to fix the stacked member 52.

[0131] The stacked component 52 is connected to the hub assembly 53 at its end face, clamping the seat plate 74 in the middle. The stacked component 52 also has a through hole 521 corresponding to the connecting hole 533 for inserting fixing screws.

[0132] In another embodiment of the invention, such as Figures 7 to 9 As shown, the axial direction of the shearing device 3 forms an angle with the bottom wall 11 of the culture tank, preferably 90°.

[0133] The shearing device 3 includes a spacer 31, a shearing part 32, a transmission part 33, a power part 34, and a flow guide 35.

[0134] The bottom wall 11 of the culture tank has a circular mounting hole, and the outer circumferential surface of the spacer 31 is cylindrical and has the same diameter as the mounting hole. The spacer 31 is detachably fitted into the mounting hole on the bottom wall 11.

[0135] One side of the spacer 31 is connected to the shearing part 32, and the other side is connected to the power part 34, which separates the shearing part 32 and the power part 34 inside and outside the culture tank. By making the cross-section of the spacer 31 larger than the cross-section of the shearing part 32, the shearing part 32 can be directly inserted through the mounting hole, thereby making it easy and quick to install the shearing device 3 on the culture tank.

[0136] In this embodiment, a shearing device 3 capable of cutting plant tissue is provided in the culture tank. At the same time, a spacer 31 is provided between the shearing part 32 and the power part 34, so that the shearing device 3 is distributedly installed inside and outside the bottom wall 11 of the culture tank. This allows the length of the plant tissue to be controlled by the shearing device 3 to maintain and promote the cultivation efficiency of the plant tissue, and also prevents pathogens or impurities from entering the culture tank through the shearing device 3, thereby improving the sealing of the culture tank.

[0137] The spacer 31 protrudes from the surface of the bottom wall 11 into the cavity 90 of the culture tank along the vertical direction of the bottom wall 11. Viewed from the outside of the bottom wall 11 of the culture tank, corresponding to the position where the spacer 31 protrudes into the cavity 90, the spacer 31 forms a concave groove on the other side.

[0138] The shearing part 32 is connected to the outer convex surface of the spacer 31, and the power unit 34 is installed in the concave groove. Preferably, the shearing part 32 is provided with a component that is sleeved on the outer periphery of the spacer 31 and can rotate relative to the spacer 31. The power unit 34 attracts and drives the component from the other side of the spacer 31 through the spacer 31, thereby driving the shearing part 32 to shear the plant tissue.

[0139] In this embodiment, by optimizing the shape of the spacer 31 so that it protrudes into the cavity 90 of the tank, the attraction and driving effect of the power unit 34 on the shearing unit 32 can be enhanced, ensuring that the shearing device 3 can better shear the plant tissue, avoid the plant tissue from tangling and clumping, and thus promote the faster growth of the plant tissue.

[0140] The spacer 31 may also have an annular groove recessed along its central axis on its outer circumference, and the middle part of the spacer 31 is located between the bottom and top of the groove along the central axis of the annular groove.

[0141] Preferably, the middle part of the spacer 31 is flush with the top of the tank, that is, the middle part of the spacer 31 is flush with the surface of the bottom wall 11 of the culture tank.

[0142] Specifically, when the spacer 31 is installed on the culture tank, the annular groove of the spacer 31 extends from the surface of the bottom wall 11 to the cavity 90 of the tank along the vertical direction of the bottom wall 11 of the culture tank, and the groove opening of the annular groove is located on the surface of the bottom wall 11 of the culture tank, so that the spacer 31 forms an annular structure with an annular groove.

[0143] Preferably, the spacer 31 protrudes into the cavity 90 of the tank at its center, forming a cylindrical structure with a cylindrical groove. In this embodiment, by optimizing the shape of the spacer 31 so that it protrudes into the cavity 90 of the tank, the attraction and driving effect of the power unit 34 on the shearing unit 32 can be enhanced, ensuring that the shearing device 3 can better shear the plant tissue, avoid the plant tissue from tangling and clumping, and thus promote faster growth of the plant tissue.

[0144] The shearing section 32 includes a first blade 323 and a second blade 324 that are respectively connected to the power section 34. The first blade 323 and the second blade 324 are disposed close to each other and can be driven by the power section 34 to rotate in opposite directions to open and close, for cutting plant tissue.

[0145] The shearing section 32 includes a pin 321 that connects the first blade 323 and the second blade 324. The second blade 324 rotates relative to the first blade 323 about the pin 321 as the central axis, and a cut is formed by the sides of the first blade 323 and the second blade 324.

[0146] The first blade 323 and the second blade 324 are elongated, and the pin 321 is vertically inserted through the center of the first blade 323 and the second blade 324. The second blade 324 rotates relative to the first blade 323 to form two symmetrical cuts relative to the pin 321.

[0147] In another connection method, the shearing part 32 includes multiple blade groups 322 connected in series on the pin 321. Each blade group 322 is formed by two first blades 323 and second blades 324 mating together and distributed at equal intervals along the axial direction of the pin 321, forming a multi-layer structure. The interval between adjacent blade groups 322 is between 1 cm and 2 cm, preferably between 1 cm and 1.5 cm.

[0148] Specifically, in the blade assembly 322, all the first blades 323 are parallel to each other and fixed to the pin 321. All the second blades 324 are parallel to each other and connected at their ends along their own length to form a frame covering the outside of the first blades 323. The second blades 324 rotate relative to the first blades 323 to form continuously opening and closing cuts.

[0149] In this way, all the second blades 324 in the blade group 322 are connected together by the frame. When the second blade 324 is driven to rotate relative to the first blade 323, the second blades 324 in the adjacent blade groups 322 rotate together relative to their respective first blades 323, forming adjacent continuously opening and closing cuts. At the same time, the plant tissue entering the cutting section 32 is cut, so that the length of the cut plant tissue is consistent with the interval between the adjacent blade groups 322.

[0150] In this embodiment, the shearing part 32 is provided with multiple sets of blades, and the blade sets 322 are arranged at fixed intervals, so that the plant tissue can be cut to obtain plant tissue of uniform length, so that the plant tissue in the culture tank has the same growth state, which facilitates the periodic adjustment of the nutrient content of the nutrient solution and the pruning of plant tissue, promotes the circulation of nutrient solution in the culture tank, maintains stable growth conditions, and improves the efficiency of cultivation and the quality of plant tissue.

[0151] The second blade 324 includes a rotating end 325 and a blade end 326. One end of the blade end 326 is connected to the rotating end 325, and the other end extends along the diameter of the rotating end 325 and is suspended on the outer circumference to form a cantilever. The rotating end 325 is sleeved on the pin 321. When the second blade 324 rotates relative to the first blade 323, the blade end 326 can rotate around the pin 321 to form a continuously opening and closing cut.

[0152] The first blade 323 has the same structure and shape as the second blade 324.

[0153] The blade end 326 includes a closed side 327 for cutting and pressing and an extended side 328 opposite to the closed side 327. The closed side 327 and the extended side 328 extend radially from the rotating end 325 to the other end and gradually approach each other.

[0154] The two ends of the closed side 327 are located on the same diameter line, and the closed side 327 extends along an arc from the rotating end 325 to the other end. Finally, an inwardly recessed notch is formed on the closed side 327 of the blade end 326.

[0155] When the second blade 324 rotates relative to the first blade 323, the cut gradually closes from both ends of the closed side 327 toward the notch.

[0156] Furthermore, the second blade 324 includes a plurality of blade ends 326 evenly distributed along the outer periphery of the rotating end 325. For example, three blade ends 326 are arranged at 120° intervals on the outer periphery of the rotating end 325; four blade ends 326 are arranged at 90° intervals on the outer periphery of the rotating end 325.

[0157] Preferably, the second blade 324 has two blade ends 326 symmetrically distributed about the rotating end 325.

[0158] Specifically, the unfolded side 328 extends outward along an arc from the rotating end 325, and the closed side 327 also extends outward along an arc from the rotating end 325. Furthermore, the unfolded side 328 and the closed side 327 gradually converge at a point.

[0159] The second blade 324 is S-shaped and consists of two blade ends 326 connected to the rotating end 325 and arranged at 180° intervals.

[0160] The blade tip 326 gradually curves upward along the circumferential direction from the closed side 327 to the unfolded side 328 to form a curved surface.

[0161] Preferably, the blade end 326 is configured as a helical surface along the diameter direction of the rotating end 325.

[0162] Preferably, the first blade 323 is elongated, and the first blade 323 has sharp corners at both ends that are inclined to its diameter, with one side of the sharp corner opposite to the closed side 327 recessed inward toward the first blade 323.

[0163] The central axis of the power unit 34 coincides with the central axis of the shearing unit 32, driving the shearing unit 32 to shear the plant tissue.

[0164] The power unit 34 is equipped with a rotor that can magnetically drive the shearing part 32 to rotate through the bottom wall 11 to open and close the cut. Specifically, a magnetic attractor made of magnetic material is fixedly installed on the rotor. The magnetic attractor can attract the shearing part 32 through the bottom wall 11 and rotate with the rotor around the central axis of the power unit 34.

[0165] In this embodiment, the power unit 34 is set to drive the shearing unit 32 with magnetic force, eliminating the traditional shaft connection structure. This eliminates the need for a sealing structure between the culture tank and the power unit 34, which not only improves the sealing performance of the bottom wall 11, but also completely prevents oil or bacteria from the power unit 34 from entering the culture tank. This ensures that the nutrient solution in the culture tank can maintain a sterile environment for a long time, providing a stable and reliable environment for the growth of plant tissues. Consequently, it greatly improves the yield and quality of plant tissues within the same culture cycle.

[0166] The power unit 34 is equipped with a magnetic attractor that can rotate around its central axis. The central axis of the power unit 34 is aligned with the central axis of the second blade 324. The magnetic attractor uses a magnetic field to hold the second blade 324 in place across the bottom wall 11, causing it to rotate together.

[0167] Alternatively, the power unit 34 includes a stator and a rotor that can be driven by the stator. The stator is fixed to the outer surface of the bottom wall 11. The rotor and stator are fitted together, with a gap between them. Introducing energy into the rotor can generate a force exerted by the stator on the rotor. This force produces a torque effect relative to the central axis, thereby driving the rotor to rotate indefinitely around the stator.

[0168] Understandably, energy sources include power sources, high-pressure gases or high-pressure liquids, and so on.

[0169] In another configuration, the rotor of the power unit 34 is annular in shape. The stator is cylindrical, and its diameter is smaller than the diameter of the annular hole in the rotor. The rotor is fitted around the outer periphery of the stator.

[0170] The magnetic attractor is fixed in the rotor on the end face facing the bottom wall 11. An easily attracted iron block is provided on the outer periphery of the second blade 324. The magnetic attractor rotates along the normal of the second blade 324 against the bottom wall 11 and attracts the second blade 324 to rotate together.

[0171] Alternatively, the power unit 34 also includes a stator and a rotor. The stator is ring-shaped and made of a strongly magnetic material. The stator has mounting holes in the middle that extend through both ends along its central axis.

[0172] The rotor is configured as a long rod. The rotor is fitted into the mounting hole of the stator and can rotate freely within the mounting hole. The magnetic attractor is fixed in the rotor at one end facing the bottom wall 11.

[0173] Preferably, a coil is wound around one end of the rotor, and the other end is connected to the magnetic attractor. The magnetic attractor is cylindrical. The second blade 324 is also provided with a cylindrical transmission part 33, one end of which is connected to the second blade 324, and the other end is sleeved on the outer periphery of the magnetic attractor.

[0174] Alternatively, the power unit 34 includes a stator and a rotor. Multiple coils are wound on the rotor. Applying electricity to the coils generates magnetic poles on the rotor that rotate about its central axis.

[0175] Specifically, multiple coils are evenly distributed around the rotor, and only one coil is energized. Then, by switching the energization between the coils in a clockwise or counterclockwise sequence, magnetic poles that continuously change direction around the rotor's central axis are generated.

[0176] The stator is made of magnetic material and generates a stable magnetic field. By energizing the rotor, rotating magnetic poles are generated, which in turn drive the rotor to rotate relative to the stator through the attraction and repulsion between the magnetic poles.

[0177] In this embodiment, coils are provided on the rotor, and magnetic poles are generated by energizing the rotor. Then, by utilizing the property that like poles repel and unlike poles attract, the energized coils are switched synchronously with the rotation of the rotor, so that the magnetic poles on the rotor are always inconsistent with the rotor's magnetic poles, thereby continuously receiving the torque applied to the rotor by the stator, which greatly improves the functional conversion efficiency of the power unit 34.

[0178] Alternatively, the power unit 34 includes a stator and a rotor, with a high-pressure medium flowing between the stator and the rotor and doing work on the rotor, causing the rotor to rotate relative to the stator.

[0179] Specifically, the stator is provided with passages for conveying high-pressure media and several chambers. The passages connect all the chambers and allow the high-pressure media to be conveyed between the chambers. At least a portion of the rotor is located between the chambers and blocks the passages. The high-pressure media flows from the passages to adjacent chambers, exerting a force on the rotor. The rotor, pushed by the high-pressure media, rotates relative to the stator.

[0180] The high-pressure medium pushes the stator from one chamber to another, losing some of its pressure. This pressure is then converted into the rotor's kinetic energy.

[0181] Preferably, the high-pressure medium includes high-pressure gas or high-pressure liquid.

[0182] The transmission unit 33 is disposed between the shearing unit 32 and the power unit 34. One end of the transmission unit 33 is connected to the shearing unit 32, and the other end is disposed close to the power unit 34. The power unit 34 transmits torque to the transmission unit 33 through magnetic force and can drive the transmission unit 33 to rotate around the pin 321.

[0183] The end of the second blade 324 in the longitudinal direction is connected to the inner circumferential surface of the transmission part 33.

[0184] Specifically, the transmission part 33 is cylindrical, with holes extending through both ends along its central axis. The second blade 324 is fitted into the holes of the transmission part 33. The two ends of the second blade 324 are connected to the inner surface of the holes, and the length direction of the second blade 324 is parallel to the diameter of the holes.

[0185] In this embodiment, the cutting part 32 is embedded in the hole of the transmission part 33. The transmission part 33 is connected to the second blade 324 but isolated from the first blade 323. This allows the second blade 324 to rotate relative to the first blade 323 and pushes the plant tissue to circulate along the axis of the transmission part 33. This avoids repeated cutting of the plant tissue, helps to ensure the length of the plant tissue, and improves the quality of the plant tissue.

[0186] The shearing section 32 and the spacer 31 are spaced apart along the axial direction of the pin 321. The end face of the shearing section 32 and the end face of the spacer 31 together with the inner circumferential surface of the transmission section 33 form a cavity for accommodating materials.

[0187] The transmission part 33 is also provided with a discharge port 333, which is formed on the side wall of the transmission part 33 and extends radially through the transmission part 33. Multiple discharge ports 333 can be formed on the transmission part 33. The discharge ports 333 are evenly distributed on the side wall of the transmission part 33 in a circumferential direction.

[0188] Specifically, in the axial direction of the transmission part 33, the discharge port 333 is located between the shearing part 32 and the spacer 31. That is, the length of the discharge port 333 along the axial direction of the transmission part 33 is less than the interval length between the shearing part 32 and the spacer 31.

[0189] The transmission section 33 is configured to consist of a discharge section 331 and a drive section 332 connected together.

[0190] Specifically, the discharge section 331 is sleeved on the pin 321. The discharge section 331 has a discharge port 333 on its side wall. The drive section 332 is sleeved on the outside of the spacer 31, and one end of the drive section 332 is flush with the top surface of the spacer 31.

[0191] Furthermore, to prevent the plant tissue from being obstructed by the sidewall of the transmission section 33 and to accelerate the flow of the plant tissue from the discharge port 333 after being cut by the shearing section 32, several discharge ports 333 are evenly distributed along the circumferential direction on the sidewall of the discharge section 331. In particular, the end of the second blade 324 is connected to the sidewall between adjacent discharge ports 333, thereby aligning the cut on the shearing section 32 with the discharge port 333.

[0192] The flow guide 35 is made of thin-walled material and is fitted around the outer periphery of the shear section 32.

[0193] The flow guide shroud 35 is also provided with parallel inlet 351 and mounting port 352. Furthermore, inlet 351 and mounting port 352 are parallel to the circular surface formed when the blade rotates.

[0194] In this embodiment, by providing a flow guide 35 around the outer periphery of the shearing section 32, the plant tissue can be promoted to flow from one side of the shearing section 32 to the other side, increasing the circulation flow speed of the plant tissue. At the same time, it can also increase and promote the circulation flow area of ​​the nutrient solution in the culture tank, ensuring that the cut plant tissue flows quickly from the shearing section 32 to other areas, avoiding the plant tissue from circulating in a small area near the shearing section 32, thereby reducing the rate of repeated cutting of plant tissue, preventing the problem of uneven plant tissue length, and effectively improving the quality of plant tissue in the culture tank.

[0195] The spacer 31 passes through the mounting port 352 of the flow guide 35 and connects to the shearing part 32. Accordingly, the inlet 351 of the flow guide 35 is located on the side of the shearing part 32 away from the spacer 31 in the axial direction of the shearing part 32.

[0196] In this embodiment, the flow guide 35 is sleeved on the outside of the spacer 31. By disassembling and assembling the spacer 31, the flow guide 35 can be easily and quickly installed on the culture tank, making the flow guide 35 easy to maintain and replace.

[0197] Additionally, the spacer 31 includes a cylindrical protrusion 311. The fairing 35 has a cylindrical hollow cover and fits over the outer circumferential surface of the protrusion 311. There is a gap between the cover surface of the fairing 35 and the outer surface of the protrusion 311. This gap forms an annular channel.

[0198] The spacer 31 is also provided with a flange edge 312 extending outward from the end face edge of the protrusion 311. The flange edge 312 is annular. The flow guide 35 is connected to the flange edge 312.

[0199] Preferably, the flow guide 35 is connected to the outer ring edge of the flange 312.

[0200] The flow guide shroud 35 is configured as a cylindrical thin-walled tube. The flow guide shroud 35 also has cavities extending through both ends along its central axis. The inlet 351 and the mounting port 352 communicate with the cavities and are located at both ends of the thin-walled tube, respectively.

[0201] Furthermore, an outlet 353 is provided on the side wall of the flow guide 35. When the flow guide 35 is fitted onto the shearing part 32, one end of the outlet 353 is aligned with the shearing part 32 along the axial direction, and the other end is close to the mounting port 352.

[0202] The inlet 351 guides the plant tissue into the incision of the shearing device 3, and the outlet 353 is used to discharge the sheared plant tissue into the culture tank.

[0203] In this embodiment, by setting a flow guide 35 around the outer periphery of the shearing part 32, the circulation flow area of ​​the nutrient solution in the culture tank is increased, which promotes the large-scale circulation of plant tissue in the cavity 90 of the tank. This avoids the plant tissue in the vicinity of the shearing part 32 from circulating only within a small range due to the influence of the shearing part 32, ensuring that the culture tank is in a good circulation state, thereby ensuring that all plant tissue can be pruned.

[0204] It should be noted that when the plant tissue enters the shearing device 3 under the action of airflow, the blades inside the shearing device 3 rotate to generate vortex suction, which draws the plant tissue into the cutting end of the shearing device 3 from the inlet 351 of the guide hood 35. The blades then cut the regularly arranged plant tissue into small segments that meet the requirements, and then discharge it from the outlet 353 of the guide hood 35. This prevents the plant tissue from clumping together and affecting the shearing effect, and ensures smooth discharge.

[0205] In embodiments of the present invention, such as Figures 1 to 3 As shown, the tank 1 includes an air inlet branch pipe 8. High-temperature steam can be introduced into the air inlet device 70 through the air inlet branch pipe 8, thereby inputting high-temperature steam into the tank 1 for cleaning and steam sterilization.

[0206] When cleaning and steam sterilizing the culture tank, water is introduced into the culture tank through the water inlet 9 at the top of the culture tank, and then steam is introduced through the air inlet device 70. The high-temperature steam disperses in the nutrient solution through the air inlet device 70 to form a large number of small bubbles, which roll in the water, greatly increasing the contact area between the high-temperature steam and the water in the culture tank, thus achieving better cleaning and steam sterilization of the culture tank.

[0207] After the culture medium is added to the culture tank, high-temperature steam is introduced into the culture tube through the air inlet device 70. Due to the function of the air inlet device 70, a large number of small bubbles are dispersed in the culture medium, which greatly increases the contact area between the high-temperature steam and the culture medium, thereby enabling the temperature of the culture medium to rise more quickly and improving the sterilization efficiency and effect of the culture device.

[0208] Furthermore, the supply unit A1 also includes: multiple media recovery pipelines; the conveying unit A2 also includes: a second main pipe and multiple media output branch pipes, with the media recovery pipelines corresponding one-to-one with the media output branch pipes, and the media in the jacket 2 is introduced into the media recovery pipelines through the media output pipelines;

[0209] Multiple output branch lines are selected to connect to the second main line 22 to export the medium in the jacket 2. Each output branch line is controllably connected to the second main line 22.

[0210] In this embodiment of the invention, the medium recovery pipeline includes a hot water recovery main pipe E100, a chilled water recovery main pipe C100, and a cooling water recovery main pipe D100 arranged in parallel, and a steam recovery main pipe, used to recover the medium output from the jacket 2 through the output pipeline;

[0211] Multiple output branch lines include: chilled water recovery branch line C10; cooling water recovery branch line D10; hot water recovery branch line E10; and steam recovery branch line.

[0212] Specifically, the hot water recovery branch pipe E10 connects to the hot water recovery main pipe E100, and the hot water introduced from the jacket 2 to the second main pipe 22 is introduced into the hot water recovery main pipe E100. The hot water supply main pipe E200 outputs hot water, which enters the first main pipe 21 through the hot water supply branch pipe E20, then enters the jacket 2 to participate in the temperature regulation work, and enters the hot water recovery branch pipe E10 through the second main pipe 22, and then returns to the hot water recovery main pipe E100, forming a temperature regulation loop.

[0213] Specifically, the steam recovery branch pipe is connected to the steam recovery main pipe, and the high-temperature steam introduced from the jacket 2 to the second main pipe 22 is introduced into the steam recovery main pipe. High-temperature steam is output from the steam supply main pipe B100, enters the first main pipe 21 through the steam supply branch pipe, then enters the jacket 2 to participate in the temperature regulation work, and enters the steam recovery branch pipe through the second main pipe 22, and then returns to the steam recovery main pipe, forming a temperature regulation loop.

[0214] Specifically, the chilled water recovery branch pipe C10 is connected to the chilled water recovery main pipe C100, and chilled water introduced from the jacket 2 into the second main pipe 22 is introduced into the chilled water recovery main pipe C100. Chilled water is output from the chilled water supply main pipe C20, enters the first main pipe 21 through the chilled water supply branch pipe C20, then enters the jacket 2 to participate in temperature regulation, and enters the chilled water recovery branch pipe C10 through the second main pipe 22, and then returns to the chilled water recovery main pipe C100, forming a cooling regulation loop.

[0215] Specifically, the cooling water recovery branch pipe D10 is connected to the cooling water recovery main pipe D100, and the cooling water introduced from the jacket 2 to the second main pipe 22 is introduced into the cooling water recovery main pipe D100. Cooling water is output from the cooling water supply main pipe D20, enters the first main pipe 21 through the cooling water supply branch pipe D20, then enters the jacket 2 to participate in the temperature regulation work, and enters the cooling water recovery branch pipe D10 through the second main pipe 22, and then returns to the cooling water recovery main pipe D100, forming a cooling regulation loop.

[0216] In this embodiment, multiple input branch lines introduce different media into the jacket 2 via a single main line. This achieves both the introduction of different media into the jacket 2 and reduces the number of inlets on the jacket 2. Different media in the jacket 2 are discharged through the same main line, and then, based on the specific media, the corresponding output branch line is selected to guide them into the corresponding recovery main line. This achieves both the differentiated recovery of different media and reduces the number of media outlets on the jacket 2.

[0217] In another embodiment, the automatically cleaning and temperature-regulating plant tissue culture device further includes a waste liquid pipeline. The output branch pipeline also includes a waste liquid output branch pipeline, which is connected to the waste liquid pipeline. This branch pipeline is used to discharge waste liquid generated within the jacket 2 or non-recyclable temperature-regulating medium into the waste liquid pipeline for waste liquid treatment. Specifically, when it is necessary to export waste liquid from the jacket 2 or when the medium exported from the jacket 2 cannot be recycled, the waste liquid output branch pipeline is opened, allowing the waste liquid to be introduced into the waste liquid pipeline and discharged through the waste liquid pipeline to the waste liquid treatment unit for waste liquid treatment.

[0218] Furthermore, both the input branch pipeline and the output branch pipeline are equipped with on / off valves 5, which control the on / off state of the input branch pipeline connected to the first main pipeline 21 and / or the output branch pipeline connected to the second main pipeline 22.

[0219] In this embodiment, the bottom of the on / off valve 5 is provided with a disinfection medium inlet for introducing high-temperature steam into the valve body to achieve disinfection and sterilization of the valve body and the medium in the pipeline.

[0220] Specifically, on / off valves 5 are installed on all input and output branch lines. The on / off valves 5 include manual valves and / or...

[0221] Alternatively, a pneumatic valve may be used. If both manual and pneumatic valves are included, they are connected in series. The appropriate valve body can be opened as needed, while valve bodies on branches that are not in operation remain closed. Simultaneously with opening the valve body, the high-temperature steam pipeline on the valve body is opened, and high-temperature steam is introduced into the opened valve body to ensure disinfection and sterilization of the valve body and branch pipelines, guaranteeing that the medium input into jacket 2 is sterile.

[0222] In an embodiment of the present invention, a temperature monitoring device is provided on the tank 1 to monitor the culture temperature inside the tank 1, and to select different media inputs by monitoring the temperature to adjust the culture temperature inside the tank 1.

[0223] Specifically, a temperature monitoring device is installed at the lower part of the tank 1 where the jacket 2 is not installed, to monitor the culture temperature inside the tank 1. By monitoring the culture temperature inside the tank 1 in real time, it can be determined what kind of medium needs to be input to ensure the stability of the culture temperature inside the tank 1 and promote the rapid growth of plant tissues.

[0224] In this embodiment of the invention, the lower edge of the jacket 2 is provided with a medium inlet, which is connected to the medium input pipeline and is used to introduce the temperature-regulating medium in the medium input pipeline into the jacket 2. The upper edge of the jacket 2 on the side opposite to the medium inlet is provided with a medium outlet, which is connected to the medium output pipeline and is used to introduce the medium in the jacket 2 into the medium output pipeline.

[0225] Specifically, a medium inlet is provided at the lower edge of the jacket 2 to cooperate with the first main pipeline 21, and a medium outlet is provided at the upper edge on the opposite side of the medium inlet to cooperate with the second main pipeline 22, thereby forming a medium circuit, reducing the number of openings on the jacket 2 while ensuring the input of suitable medium.

[0226] Specifically, the temperature inside the plant tissue culture tank 1 is detected by a temperature monitoring device. Then, a suitable heat transfer medium is selected and input into the jacket 2 based on the monitored temperature. The on / off valves 5 on the corresponding input branch pipe and the on / off valves 5 on the output branch pipe are opened according to the selected medium. The selected medium is input from the input branch pipe, and the medium circulates in the jacket 2 and is discharged from the corresponding output branch pipe, thereby realizing the temperature regulation of the plant tissue culture tank and the circulation of the temperature regulating medium.

[0227] In another embodiment, when the medium entering the jacket 2 is not recycled upon discharge, the waste liquid output branch pipe is opened. The waste liquid output branch pipe is connected to the waste liquid pipeline. At this time, the medium used in the jacket 2 will not enter the medium recovery main pipe, but will be introduced into the waste liquid pipeline through the waste liquid output branch pipe. Then, after being cooled and depressurized in the waste liquid treatment section, waste liquid treatment is carried out.

[0228] like Figures 1 to 3 In another embodiment of the present invention, the plant cultivation device includes a seed tank and a fermentation tank. The seed tank and fermentation tank of the plant cultivation device can be made of any material suitable for preparing fermentation tanks and can be used for high-temperature sterilization, such as glass, stainless steel, high-temperature resistant plastics, etc.; stainless steel is preferred because it is durable and has a long service life.

[0229] For ease of distinction, smaller plant tissue culture tanks are called seed tanks, while larger plant tissue culture tanks are called fermentation tanks.

[0230] In order to reduce the number of times the cultivated adventitious roots and buds are inoculated into the culture device and to achieve the purpose of multiple cycles of proliferation of adventitious roots and buds, the plant tissue culture tanks used to cultivate plant tissues or cells are divided into seed tanks and fermentation tanks.

[0231] The seed tank is used to cultivate adventitious roots and buds, which serve as seeds for the rapid cultivation and proliferation of adventitious roots and buds in the next fermentation cycle.

[0232] In particular, the seed tank is smaller than the fermentation tank, being 1 / 3 to 1 / 5 the volume of the fermentation tank.

[0233] Specifically, a transfer pipeline and a return pipeline are installed between the seed tank and the fermentation tank. The transfer pipeline introduces the culture medium from the seed tank into the fermentation tank through a connection port on the fermentation tank for continued cultivation. The return pipeline introduces the culture medium transferred from the fermentation tank back into the seed tank through a connection port on the seed tank.

[0234] Specifically, a jacket 2 is provided on the outer side of the peripheral wall of the tank 1. The upper part of the jacket 2 is located below the connection port, and the lower part of the jacket 2 extends to the inclined bottom wall 11, located above the air inlet device 70 and the shearing device 3. A temperature regulating medium can be introduced into the jacket 2 to adjust the culture temperature in the tank 1 to a suitable range of culture temperature.

[0235] like Figure 10 As shown, at least two air intake devices 70 and two shearing devices 3 are provided on the bottom wall 11 of the seed tank. The shearing devices 3 and the air intake devices 70 are alternately distributed on the bottom wall 11, and the installation position of the shearing devices 3 is higher than that of the air intake devices 70 in the vertical direction.

[0236] Preferably, the line connecting the installation positions of the two shearing devices 3 and the line connecting the installation positions of the two air intake devices 70 pass through the center of the seed tank.

[0237] The aeration sections 72 of the two air intake devices 70 of the present invention are preferably arranged on the same plane during installation and their extension directions are approximately parallel in projection within the seed tank, and are arranged symmetrically with respect to the center of the bioreactor.

[0238] like Figure 10 As shown, the aeration section 72 has an extended length. One end, connected to the conduit 73, is spaced from the bottom wall 11, while the other end extends towards the center of the bioreactor, tangent to or intersecting the centerline of the bioreactor. The distance between the end of the aeration section 72 connected to the conduit 73 and the bottom wall 11 is smaller than the distance between the other end of the aeration section 72 and the bottom wall 11. That is, the length of the conduit 73 extending into the culture tank is relatively short, just enough to allow the aeration section 72 to be installed on the bottom wall 11 and its installation angle to be adjusted.

[0239] The gas supplied from the aeration section 72 has a certain pressure, which causes the culture medium and plant tissue in the bioreactor to rise along the area near the central axis, then diffuse to the periphery, and then circulate along the outer parabolic trajectory near the inner wall of the bioreactor. During the process of rising and falling along the near parabolic trajectory, the culture medium applies a certain force to the plant tissue, causing it to be arranged and flow in a basically parabolic trajectory in the length direction.

[0240] The shearing device 3 has gaps between its ends extending toward the centerline of the bioreactor, allowing the culture medium and plant tissue rising near the central axis to pass through.

[0241] The plant tissues arranged in the same direction flow downwards into the incision of the shearing device 3 under the action of parabolic airflow inside the bioreactor.

[0242] The shearing device 3 and the air inlet device 70, arranged in this way, can provide uniformly distributed gas into the bioreactor. Because the aeration section 72 has a higher distribution ratio at the center line of the bioreactor than at the bottom wall 11, the kinetic energy provided by the gas in the center line region is greater than the potential energy of the culture medium and the pressure above it. The culture medium can move upwards under the influence of the airflow. When it reaches a certain height, it is farther from the aeration section 72, and the kinetic energy generated by the gas on the culture medium weakens, becoming less than the potential energy of the culture medium and the pressure above it. Simultaneously, the potential energy of the culture medium and the pressure above it at the inner wall are greater than the kinetic energy provided by the gas. This allows the culture medium to circulate along a parabolic trajectory, rising near the central axis region, then diffusing outwards and descending near the inner wall of the bioreactor. Due to the gravity of the culture medium, the lower... The near-vertical descent trajectory increases the contact area between the gas, culture medium, and plant tissue, improving gas dissolution and preventing plant tissue decay without requiring excessive stirring. Furthermore, the culture medium's near-parabolic trajectory provides a driving force to the plant tissue, causing it to align regularly in the same direction, preventing tangling and hindering growth efficiency. The moving culture medium guides the plant tissue into a regular arrangement and into the incision of the shearing device 3. Simultaneously, the rotating blade in the shearing device 3 generates a vortex suction force, further drawing the regularly arranged plant tissue into the incision, ensuring uniform shearing and preventing uneven shearing of tangled plant tissue or repeated shearing.

[0243] Furthermore, the angle β between the shearing device 3 and the bottom wall 11 is adjustable. The aeration section 72 and the shearing device 3 extend along their respective length directions and intersect at a point to generate an angle β1. The angle between the bottom wall 11 and the horizontal plane is β2, and β1+β2=β is satisfied. Preferably, when the angle β is 90 degrees, the air intake effect of the aeration section 72 is optimal.

[0244] Similarly, such as Figure 11 As shown, the bottom wall 11 of the fermenter is provided with at least four air inlet devices 70 and two shearing devices 3, which are arranged in a similar manner to the seed tank.

[0245] Four air intake devices 70 are evenly arranged around the bottom wall 11, with equal spacing. The aeration section 72 of the air intake device 70 is horizontal and has a certain length. One end of any two opposing sets of aeration sections 72 connected to the conduit 73 is spaced from the bottom wall 11, while the other end is tangent to or intersects the centerline of the bioreactor. Their projections within the fermenter are approximately parallel and symmetrically arranged with respect to the center of the bioreactor; the projections of two adjacent sets of aeration sections 72 within the fermenter are approximately perpendicular.

[0246] A shearing device 3 is provided at intervals between every two air intake devices 70. The installation position of one shearing device 3 on the same side of the bottom wall 11 and the line connecting the installation positions of the two adjacent air intake devices 70 in the fermenter approximately form an isosceles triangle. The installation position of the shearing device 3 is higher than the air intake device 70 in the vertical direction.

[0247] By rationally setting the relative positions of the shearing device 3 and the air intake device 70, the gas provided by the aeration section 72 of the air intake device 70 causes the culture medium and plant tissue in the bioreactor to rise along the area near the central axis, then diffuse to the periphery, and circulate along the trajectory of an outer parabolic surface near the inner wall of the bioreactor. During the process of rising and falling along the trajectory of the near parabolic surface, the culture medium applies a certain force to the plant tissue, causing it to be arranged and flow in a basically parabolic trajectory in the length direction.

[0248] Alternatively, the aeration section 72 of the air intake device 70 is disposed close to the bottom wall 11, and the gas provided from the aeration section 72 causes the culture medium and plant tissue in the bioreactor to circulate along the trajectory of an inner parabolic surface that rises near the inner wall of the bioreactor and falls near the central axis region.

[0249] As the culture medium rises and falls along a near-parabolic trajectory, it applies a certain force to the plant tissue, causing it to arrange and flow in a basically parabolic trajectory along its length. This facilitates cutting the tissue into smaller segments using the shearing device 3, preventing it from becoming tangled and repeatedly cut.

[0250] The shearing device 3 includes a rotatable cutter, into which the culture medium guides regularly arranged plant tissues as it descends along a near-parabolic trajectory.

[0251] The cutting blade comprises two blades distributed at the top and bottom, which cut most of the plant tissue into pieces the size of the distance between the two blades.

[0252] The shearing device 3 has an angle with the bottom wall 11, and the shearing plane where the double blades are located has an angle with the trajectory of the plant tissue descending along a parabolic shape.

[0253] In this embodiment, the shearing device 3 and the bottom wall 11 have an angle, preferably 90°, that is, the shearing device 3 is vertically set on the bottom wall 11. At this time, the shearing plane where the double blades are located forms an acute angle with the trajectory of the parabolic descent. As the culture medium descends along the trajectory close to the parabolic shape, it guides the regularly arranged plant tissues into the cutter. Thus, when the shearing device 3 uses the cutter to cut the plant tissues into small segments, the shearing surface is an inclined plane. The increased area of ​​the shearing surface can cultivate more plant tissue seeds, such as ginseng adventitious roots, improving production efficiency. Moreover, the shearing uniformity is high, preventing uneven shearing of entangled plant tissues or repeated shearing of plant tissues. Cutting into uniform small segments makes it easier to uniformly control the degree of production and also facilitates material discharge, preventing entangled plant tissues from clogging the pipes.

[0254] Alternatively, the installation angle of the shearing device 3 can be adjusted so that the shearing plane of the double blades is approximately perpendicular to the trajectory of the parabolic descent. In this way, the plant tissue can be directly aligned with the cut of the shearing device 3, increasing the number of plant tissues entering the shearing device 3 each time, improving shearing efficiency, and ensuring that the plant tissue is cut into small segments of approximately the same length and uniformity.

[0255] Furthermore, the temperature-regulating medium output from the medium supply main pipe is divided into two branch pipes after passing through a main pipe, which are respectively connected to the input main pipes set on the seed tank and the fermentation tank, namely the first main pipe 21 mentioned in the above embodiment.

[0256] Specifically, different temperature-regulating media are output from the corresponding media supply main pipe through the corresponding main pipe and then enter the corresponding temperature-regulating media branch pipes. After passing through the branch pipes, they converge to the input main pipe connecting the seed tank and the fermentation tube, and are input into the corresponding jacket 2 through their respective input main pipes, thereby regulating the temperature of the seed tank and the fermentation tank.

[0257] More specifically, different media are output from the main supply line and then enter the main input lines of the seed tank and fermentation tank through two branch lines respectively. They then enter the jacket 2 through the main input lines to regulate the temperature of the plant tissue culture tank. After flowing in the jacket 2, the temperature regulating media are output through the main output line of the jacket 2 and enter the corresponding media recovery main line through the corresponding output branch line to recover the media, or enter the waste liquid pipe to treat the media as waste liquid.

[0258] Furthermore, when the plant tissue culture device starts culturing, the on / off valve 5 on the steam branch line is opened to introduce steam into the jacket 2 of the tank 1; during the culturing process, the on / off valve 5 on the branch line of the corresponding temperature-regulating medium is opened as needed to introduce the corresponding medium and adjust the culturing temperature in the tank 1.

[0259] Specifically, before adding seeds to the culture medium in the culture device, steam is first introduced into the jacket 2 on tank 1 and into tank 1. The on / off valve 5 on the steam input branch pipe is opened to introduce high-temperature steam into the jacket 2, causing tank 1 to heat up rapidly to a temperature suitable for plant tissue culture. The on / off valve 5 on the air inlet branch pipe 8 is then opened to introduce high-temperature steam into tank 1 to sterilize the culture medium in tank 1. The high-temperature steam introduced into the jacket 2 not only heats tank 1 but also assists in sterilizing the culture medium in tank 1. Then, the on / off valve 5 on the high-temperature steam branch pipe is closed, and seeds are placed into tank 1 of the seed tank for cultivation.

[0260] During the cultivation process, the temperature inside tank 1 is monitored by a temperature monitoring device. Based on temperature changes, the on / off valve 5 on the corresponding temperature-regulating medium branch pipe is opened as needed to adjust the temperature of tank 1 and ensure a suitable growth temperature for adventitious roots. However, when the cultivation tank is needed, steam is first introduced into the jacket 2 of the cultivation tank to rapidly raise its temperature to the seed cultivation temperature. Then, seeds are transferred from the seed tank to the cultivation tube. During the cultivation process, the temperature of tank 1 is monitored by the temperature monitoring device, and based on temperature changes, the on / off valve 5 on the corresponding heat transfer medium branch pipe is opened as needed to adjust the temperature of tank 1 and ensure a suitable growth temperature for the plant tissues.

[0261] While the on / off valve 5 of the aforementioned input or output branch line is opened, a disinfection medium is introduced into the on / off valve 5 to disinfect and sterilize the on / off valve 5 and the introduced medium on the branch line.

[0262] In another embodiment of the present invention, a temperature regulation method for a temperature regulation system is provided, including steps S10 to S30.

[0263] In step S10, during the cultivation process, the temperature monitoring device monitors the temperature T inside the culture tank;

[0264] In step S20, the monitored temperature T is compared with a preset threshold T0;

[0265] In step S30, if the monitored temperature T exceeds the preset threshold T0, the on / off valve on the corresponding branch pipe is opened to introduce the temperature regulating medium.

[0266] The preset threshold T0 is a range value, and values ​​exceeding the preset threshold are considered to be outside this range.

[0267] Optionally, in step S30, if the temperature T is higher than the preset threshold T0, the on / off valve 5 on the chilled water supply branch C20 and chilled water recovery branch C10 / cooling water supply branch D20 and cooling water recovery branch D10 is opened to allow chilled water / cooling water to flow in for cooling regulation.

[0268] Optionally, in step S30, if the temperature T is lower than the preset threshold T0, the on / off valve 5 on the hot water supply branch pipe E20 and the hot water recovery branch pipe E10 / steam supply branch pipe and the steam recovery branch pipe is opened to introduce hot water / high-temperature steam for temperature adjustment.

[0269] Specifically, during the plant cultivation process, a temperature monitoring device installed on the cultivation tank monitors the temperature inside the tank 1 and compares the monitored temperature with a preset threshold. The comparison result is then used to adjust the temperature by inputting different media.

[0270] It should be noted that, in this embodiment, the preset threshold is a suitable temperature range for plant cultivation.

[0271] Optionally, when the monitored temperature value is higher than the highest value of the preset threshold range, cooling water or chilled water is input to lower the temperature; when the monitored temperature is lower than the lowest value of the preset threshold, hot water or high-temperature steam is input to raise the temperature.

[0272] Optionally, when temperature regulation is performed, different media are introduced into the jacket 2. After the media completes temperature regulation, it does not return to the main media recovery pipe. Then, the on / off valve 5 on the waste liquid output branch pipe is opened. The temperature-regulating medium is introduced into the jacket 2 from the corresponding input branch pipe. After flowing and temperature regulation, it is discharged through the waste liquid output branch pipe and enters the waste liquid treatment unit for waste liquid treatment.

[0273] In a second aspect, the present invention provides a control method for an automatic cleaning and temperature-regulating plant tissue culture device, applied to the aforementioned automatic cleaning and temperature-regulating plant tissue culture device, the method comprising: steps S100 to S300.

[0274] In step S100, before adding culture medium to the culture tank, the cleaning system is controlled to introduce water into the culture tank through the water inlet on the culture tank to clean the culture tank.

[0275] In step S200, after the cleaning is completed, the used water is drained.

[0276] In step S300, steps S100 to S200 are repeated at least three times to add culture medium and plant tissue seeds to the culture tank for culture.

[0277] Optionally, step S100 includes at least: the control system controls the air intake device at the bottom of the culture tank to allow air to enter, and simultaneously controls the shearing device to operate, thereby completing the simultaneous cleaning of the shearing device.

[0278] Optionally, in step S300, during the final cleaning, while controlling the water inlet on the culture tank to allow water to enter the culture tank, the air inlet branch valve is opened to allow steam to be introduced into the culture tank through the air inlet device for cleaning and steam sterilization.

[0279] Optionally, in step S300, after the final cleaning is completed, the following steps are included:

[0280] S301. Add culture medium to the culture tank;

[0281] S302. Steam is introduced into the culture tank through the air inlet device for steam sterilization;

[0282] S303, The temperature control and adjustment system regulates the temperature of the culture device to a suitable culture temperature;

[0283] S304. Plant tissue seeds are added for culture, and a temperature control system is used to regulate the temperature during the culture process.

[0284] In this embodiment of the invention, the culture tank needs to be cleaned before plant tissue culture. The control system controls the cleaning system to introduce water into the culture tank through the water inlet 9, which is set in the culture tank, to clean the culture tank. After cleaning, the water is drained, completing the first cleaning of the culture tank. To ensure that there are no residues in the culture tank, the culture tank is cleaned at least three times.

[0285] It should be noted that cleaning the culture tank before plant tissue culture includes cleaning the culture tank when using it for the first time, as well as cleaning the culture tank when changing the culture medium after one culture is completed.

[0286] During the cleaning process, the control system controls the air intake device 70 and the shearing device 3 to work. Gas is introduced through the air intake device 70, and the gas forms tiny bubbles as it passes through the air intake system, which can better clean the culture tank. At the same time, the control system controls the shearing device 3 to work, which can clean the shearing device 3 at the same time, thus achieving a comprehensive cleaning of the culture tank.

[0287] In this embodiment of the invention, during the final cleaning, the control system controls the cleaning system to take in water from the inlet 9 of the culture tank, and at the same time controls the high-temperature steam supply pipeline B100 to provide high-temperature steam. The high-temperature steam is input into the air intake device 70 through the air intake branch pipe 8, and then enters the culture tank through the air intake device 70 to clean and steam sterilize the culture tank.

[0288] After cleaning and steam sterilization of the culture tank, culture medium is added, and steam is introduced into the tank through the air inlet device 70 to sterilize the culture medium and ensure a sterile culture state. The control system controls the temperature adjustment system to regulate the temperature by inputting the corresponding temperature-regulating medium, lowering the temperature of the culture tank to the suitable temperature for plant tissue culture.

[0289] After the culture medium is steam sterilized, plant tissue seeds are added to the culture tank for cultivation. During the cultivation process, the temperature monitoring device installed on the culture tank detects the temperature inside the culture tank. Based on the temperature monitoring results, the control system controls the temperature adjustment system to introduce the required medium into the jacket 2 to regulate the temperature of the culture tank and ensure that the temperature during the plant cultivation process is suitable for the growth of plant tissue seeds.

[0290] The above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention in any way. Although the present invention has been disclosed above with reference to preferred embodiments, it is not intended to limit the present invention. Any person skilled in the art can make some modifications or alterations to the above-described technical content to create equivalent embodiments without departing from the scope of the present invention. The implementation schemes in the above embodiments can also be further combined or replaced. Any simple modifications, equivalent changes and alterations made to the above embodiments based on the technical essence of the present invention without departing from the scope of the present invention shall still fall within the scope of the present invention.

Claims

1. An automatic cleaning and temperature-regulating plant tissue culture device, characterized in that, include: The culture tank includes a jacket fitted over the outside of the tank body, an air inlet device located at the bottom of the tank body, and a shearing device located above the air inlet device. The air intake device includes an air intake section, a conduit, and an aeration section. The aeration section has a central cavity that communicates with the conduit, and the cavity wall has micropores that connect from the outside of the aeration section to the inside of the cavity. A seat plate is positioned between the air intake section and the aeration section, separating them. The seat plate is a circular flat plate. The conduit is a straight rod, including a long, straight air intake section that extends at least a certain length to both sides of the seat plate. The air intake device includes a mounting assembly that can rotate at any angle around its central axis when connected to the culture tank. This mounting assembly includes an annular stacked component, with the stacked component and the interface on the culture tank connected at their end faces, and the seat plate is clamped between them to securely mount the air intake device. The shearing device includes a shearing section, which includes a first blade and a second blade respectively connected to a power unit. The first blade and the second blade are disposed close together and are driven by the power unit to rotate in opposite directions to open and close, for cutting plant tissue. The shearing device includes a spacer, a shearing section, a transmission section, a power unit, and a flow guide. The spacer is detachably fitted into a mounting hole on the bottom wall of the culture tank. One side of the spacer is connected to the shearing section, and the other side is connected to the power unit, for separating the shearing section and the power unit inside and outside the culture tank. The transmission section is located between the shearing section and the power unit, with one end connected to the shearing section and the other end located close to the power unit. A flow guide is provided on the outer periphery of the shearing section. A temperature adjustment system, comprising a supply unit, a conveying unit, and a temperature-regulating water tank connected to the supply unit, wherein the supply unit carries multiple temperature-regulating media of different temperatures and introduces them into and out of the jacket and / or temperature-regulating water tank via the conveying unit, thereby adjusting the culture temperature of the plant tissue culture device. The cleaning system includes a water inlet on the culture tank and an air inlet branch pipe for introducing steam into the air inlet device; The control system is communicatively connected to the culture tank, temperature control system, and cleaning system. The control system controls the temperature adjustment system to select the temperature-regulating medium entering the jacket to adjust the temperature of the culture device; controls the cleaning system to introduce water into the culture tank through the water inlet for water cleaning; controls the air intake device at the bottom of the culture tank to introduce air; and simultaneously controls the shearing device to work to clean the shearing device; and / or introduces steam into the culture tank through the air intake device to disperse and form small bubbles for cleaning and steam sterilization of the culture tank.

2. The automatic cleaning and temperature-regulating plant tissue culture device according to claim 1, characterized in that, The temperature-regulating water tank is provided with a water-containing cavity. The water-containing cavity and the jacket are connected to the supply unit through a conveying unit to form a circulation loop. The conveying unit includes an inlet pipe and an outlet pipe that connect the supply unit and the water-containing cavity. A water pump is connected in series in the inlet pipe to convey the water in the water-containing cavity to the jacket. The aeration section of the air intake device is located inside the cavity of the culture tank, and the air intake section extends out of the outside of the culture tank; micropores are evenly distributed throughout the cavity wall. The shearing section includes a pin that connects the first blade and the second blade in series. The second blade rotates relative to the first blade about the pin as a central axis, and a cut is formed by the sides of the first blade and the second blade. Alternatively, the shearing section may include multiple blade sets connected in series on the pin shaft. Each blade set consists of two pairs of the first blade and the second blade, which are distributed at equal intervals along the axial direction of the pin shaft to form a multi-layer structure.

3. The automatic cleaning and temperature-regulating plant tissue culture device according to claim 2, characterized in that, The temperature adjustment system further includes: a heat exchange structure, and a central control unit electrically connected to pneumatic valves connected in series in each branch of the liquid inlet pipe. The central control unit is used to control the ratio of chilled water and high-temperature steam delivered from the liquid inlet pipe to the heat exchange structure of the temperature-regulating water tank. The heat exchange structure is arranged close to the water-containing cavity.

4. The automatic cleaning and temperature-regulating plant tissue culture device according to claim 3, characterized in that, The supply unit includes multiple media supply pipelines, including a hot water supply main pipe connected in parallel for supplying media at a first temperature; a high-temperature steam supply main pipe for supplying media at a second temperature; a chilled water supply main pipe for supplying media at a third temperature; and a cooling water supply pipe for supplying media at a fourth temperature. Third temperature < Fourth temperature < First temperature < Second temperature; The delivery unit includes multiple parallel medium input branch pipelines and a first main pipeline, with one of the medium input branch pipelines being connected to the first main pipeline. Multiple media input branch lines correspond one-to-one with multiple media supply lines, allowing the media at a preset temperature to be input into the jacket at one of them; The duct is also provided with an air outlet section at an angle to the air inlet section. One end of the air outlet section is connected to the air inlet section and the other end is connected to the aeration section. The angle between the air outlet section and the air inlet section is greater than 90°. One side of the spacer protrudes into the tank; the shearing part is connected to the end of the spacer that protrudes into the tank; The septum protrudes from the surface of the bottom wall into the cavity of the culture vessel along the vertical direction of the bottom wall of the culture vessel; The shearing part is connected to the outer convex surface of the spacer, and the power part is installed in the concave groove.

5. The automatic cleaning and temperature-regulating plant tissue culture device according to claim 4, characterized in that, The high-temperature steam supply pipeline has multiple branches, one of which is connected to the air inlet branch pipe, which is connected to the air inlet device. Steam is introduced into the culture device through the air inlet device to clean and sterilize the culture device. The transmission section is cylindrical and consists of a discharge section and a drive section connected together. The discharge section is sleeved on the pin, and the drive section is sleeved on the outside of the spacer. The discharge section has a discharge port on its side wall. The shearing device and the air intake device are alternately distributed on the bottom wall of the culture tank, and the shearing device is installed at a position higher than the air intake device in the vertical direction.

6. The automatic cleaning and temperature-regulating plant tissue culture device according to claim 4, characterized in that, The supply unit also includes: multiple media recovery pipelines; The delivery unit also includes: a second main pipe and multiple media output branch pipes, with a media recovery pipe corresponding to each of the media output branch pipes. The media in the jacket is introduced into the media recovery pipe through the media output branch pipes. Multiple media output branch lines are selectively connected to the second main line to export the media from the jacket; each output branch line is controllably connected to the second main line. The plant tissues are adventitious roots and adventitious buds.

7. A control method for an automatic cleaning and temperature-regulating plant tissue culture device, characterized in that, The method, applied to an automated cleaning and temperature-regulating plant tissue culture apparatus as described in any one of claims 1-6, comprises: S100. Before adding culture medium to the culture tank, control the cleaning system to introduce water into the culture tank through the water inlet on the culture tank to clean the culture tank. S200. After cleaning, drain the used water. S300, Repeat steps S100 to S200 at least three times. In step S100, the control system controls the air intake device at the bottom of the culture tank to allow air to enter, and at the same time controls the shearing device to work, so as to clean the shearing device simultaneously. Culture medium and plant tissue seeds are added to the culture tank for cultivation.

8. The control method according to claim 7, characterized in that, In step S300, during the final cleaning, while controlling the water inlet on the culture tank to allow water to enter the culture tank, the air inlet branch valve is opened to allow steam to be introduced into the culture tank through the air inlet device. The high-temperature steam is dispersed through the air inlet device to form small bubbles, which tumble in the water to perform cleaning and steam sterilization.

9. The control method according to claim 7, characterized in that, High-temperature steam disperses into the nutrient solution through the air intake device, forming small bubbles, while simultaneously controlling the operation of the shearing device to clean it.

10. The control method according to claim 7 is characterized in that, In step S300, after the final cleaning is completed, the following steps are included: S301. Add culture medium to the culture tank; S302. Steam is introduced into the culture tank through the air inlet device for steam sterilization; S303, The temperature control and adjustment system adjusts the temperature of the culture device to a suitable culture temperature; S304. Plant tissue seeds are added for culture, and a temperature control system is used to regulate the temperature during the culture process.

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